阅读说明：本技术 舌岩白菜提取物的应用 (Application of bergenia mellifera extract ) 是由 姚期凤 姜丹 吴越 王越 蒋耀权 章漳 于 2018-05-29 设计创作，主要内容包括：本发明公开了一种舌岩白菜提取物的应用,具体为在制备抗皮肤衰老基因的表达上调剂中的应用。所述的抗皮肤衰老基因可为COL3A1基因、FBN1基因、SIRT1基因和NID1基因中的一种或多种。所述的抗皮肤衰老基因的表达上调剂可以增强和支持皮肤组织结构的稳固性,延缓皮肤衰老,可用于化妆品或者护肤品中。(the invention discloses an application of a bergenia purpurascens extract, in particular to an application in preparing an expression up-regulator of an anti-skin aging gene. The skin aging resistant gene can be one or more of COL3A1 gene, FBN1 gene, SIRT1 gene and NID1 gene. The expression up-regulator of the anti-skin aging gene can enhance and support the stability of the skin tissue structure and delay skin aging, and can be used in cosmetics or skin care products.)

1. application of Bergenia pacifica (Bergenia pacificus) extract in preparing an expression up-regulator of anti-skin aging genes is provided.

2. The use of claim 1, wherein: the anti-skin aging gene is one or more of COL3A1 gene, FBN1 gene, SIRT1 gene and NID1 gene.

3. The use of claim 1, wherein: the bergenia tenacissima extract is a bergenia tenacissima alcohol extract, can also be a bergenia tenacissima C1-C5 alcohol extract, and can also be a bergenia tenacissima alcohol extract.

4. the use of claim 1, wherein: the bergenia tenacissima extract is the root extract of bergenia tenacissima.

5. the use of claim 1, wherein: the preparation method of the bergenia purpurascens extract comprises the following steps:

(1) Extracting powder of bergenia purpurascens with ethanol, and filtering to obtain filtrate;

(2) Drying the filtrate.

6. The use of claim 5, wherein: the powder of bergenia purpurascens is powder of bergenia purpurascens root;

And/or the mesh number of the bergenia purpurascens powder is 20-60 meshes;

And/or the ethanol is 70-95% ethanol;

And/or the addition amount of the ethanol is 8-12 times of the volume of the bergenia schreberi powder;

And/or, the temperature of the extraction is 20-30 ℃;

And/or, the extraction is ultrasonic extraction;

And/or, the extraction times are more than 1;

and/or the filtration method is centrifugation.

7. The use of claim 1, wherein: the preparation method of the bergenia purpurascens extract comprises the following steps:

(1) Adding 70% ethanol 10 times the volume of the powder of root of bergenia purpurascens into the powder of root of bergenia purpurascens, performing 500W ultrasonic extraction at 20-30 deg.C for 2 times, each for 30min, centrifuging, filtering, and mixing filtrates; the speed of the centrifugation is 8000rpm, and the time of the centrifugation is 10 minutes;

(2) And evaporating the filtrate to dryness.

8. the use of claim 7, wherein: the concentration of the bergenia purpurascens extract in the expression up-regulator of the skin aging resistant gene is 8-120 mug/mL, and can also be 10-100 mug/mL.

9. The use of claim 8, wherein: the concentration of the bergenia purpurascens extract in the expression up-regulator aiming at the FBN1 gene and/or NID1 gene is 8-120 mug/mL, and can also be 10-100 mug/mL;

And/or the concentration of the bergenia purpurascens extract in the expression up-regulator aiming at the COL3A1 gene and/or the SIRT1 gene is 80-120 mu g/mL, and can also be 100 mu g/mL.

10. Use according to any one of claims 1 to 9, wherein: the expression up-regulator of the anti-skin aging gene is used in cosmetics or skin care products.

Technical Field

the invention relates to the field of plant extracts and daily chemicals, in particular to application of a bergenia extract.

Background

The skin covers the surface of a human body and is in direct contact with the external environment, so that the human body can be protected from being damaged by various external factors, and the human body can be used as a mirror to reflect the health state of the human body. The skin is composed of epidermis, dermis, and subcutaneous tissue, and contains skin appendages, abundant nerves, blood vessels, lymphatic vessels, and muscles. The connective extracellular matrix region of the dermis and epidermis, also known as the Basement Membrane (BM), not only supports the epidermis, but also serves multiple functions, such as the junctions of the dermis and epidermis acting as a selective cellular barrier that generally prevents connective tissue cells from contacting the epidermal cells of the Basement membrane, but allows passage of lymphocytes, macrophages, and neuronal synapses; in addition, many vital phenomena such as cell morphology, cell polarity, cell metabolism, protein distribution in plasma membranes, cell survival, cell proliferation and differentiation, and cell migration are very closely related to the basement membrane. Nestin is an important component of basement membrane and is essential for maintaining the structure and function of basement membrane, which is essential for maintaining normal renewal of skin. The dermis is composed of cells and extracellular matrix, and fibroblasts are main cells of the dermis, and the normal proliferation and differentiation of the fibroblasts maintain the structure of the dermis of the skin and form normal structure and physiological functions; extracellular matrix is a kind of macromolecular substance synthesized and secreted by cells and interacting around the cells, and many life phenomena such as proliferation and differentiation of cells, cell migration and the like are closely related to the extracellular matrix. The extracellular matrix is composed of four major classes of molecules: collagen, elastin, glycoprotein and proteoglycan. The extracellular matrix is finely and skillfully entangled and interwoven through different molecules to form various exquisite three-dimensional structures, so that the skin is endowed with elasticity and tensile strength, and looks plump and full of vitality.

NID1(Nidogen) gene encodes protein nestin 1, which is important protein of basement membrane and plays a role in connecting type iv collagen and laminin 5, and is essential for the structure and function of basement membrane. Whether the structure and function of the basement membrane are normal or not determines whether the skin can be normally renewed and differentiated, the nestin expression is reduced, the basement membrane structure is damaged, the proliferation and differentiation of epidermal cells are influenced, and the skin is aged.

Fibrillin 1 encoded by FBN1(Fibrillin 1) gene is secreted by fibroblasts of dermis into extracellular matrix, and can be used as structural component of calcium-binding microfibril to provide stress structure for elastic and inelastic connective tissues of whole body and mediate a series of cell and matrix interactions. Is an important component of elastic fiber in connective tissue, in the process of forming the elastic tissue, the fibrillin 1 provides a skeleton for storing the elastin, the periphery of the mature elastic fiber grows, the reduction of the skin elasticity is an important characteristic of skin aging, the expression level of the fibrillin 1 is closely related to the skin elasticity, and the reduction of the expression level of the fibrillin 1 is also a key factor causing the skin aging.

the COL3a1(collagen type III alpha 1chain) gene encodes type III collagen, which encodes the pre- α 1chain of type III collagen, a fibrillar collagen, which is commonly associated with type I collagen, and can be found in the connective tissues of the extensible body such as skin, lung, uterus, intestine and vascular system, and the production of type III collagen helps to strengthen and support the firmness of the skin tissue structure, delaying skin aging.

SIRT1(sirtuin 1): one of the famous longevity genes. SIRT1 is a highly conserved protein deacetylase, which participates in regulation of longevity caused by energy restriction and is called a longevity gene; the SIRT1 has complex physiological action, participates in regulation of glycolipid metabolism, inflammation, oxidative stress, cell aging, apoptosis and the like, and is closely related to skin aging.

Therefore, the research on how to improve the expression of genes related to key parts and functions of the skin has important significance for improving the skin repair capacity, maintaining the structural stability and delaying skin aging.

Bergenia pacifica, (Bergenia pacifica) yanqi (yunnan lincang) plate 9: 1-4. Is grown in the shady and humid place in the miscellaneous trees with elevation of 2700 and 4800m, or on the grass slope with rocks or in the stone cracks, and is distributed in the places of Sichuan, Yunnan, Tibet and the like. Saxifragaceae, perennial herbs, about 17 cm high. The rootstock is thick and strong, and is squashed and leaves are remained. The leaves are all grown; the leaf leather is round, wide oval to wide inverted oval, the length is 7-15 cm, the width is 6.6-13.2 cm, the tip is blunt, the whole edge or the edge is near to the tip is provided with unobvious round teeth, the base is heart-shaped, and both sides have no hair; the leaf stalk is 3-10 cm long and above the leaf supporting sheath, and the length of the leaf stalk can exceed 1 cm; the edge of the leaf supporting sheath is provided with hard eyelashes. Scape has no hair. The polypodium is conical and is about 7.5 cm long; the pedicel is about 5 mm long, and is separated from inflorescence branches to generate glandular hair with nearly no stalk; the lower part of the outer surface of the supporting cup is used for dredging gland hair which is close to stem-free; the sepals are developed in the flowering period, are leathery, wide in egg shape and 3-3.3 mm long, and the tip of the edge sometimes has sparse teeth, the whole edge below the sparse teeth is hairless and has multiple veins; the petals are white or pink, are approximately round, have the length of about 8.5 mm, the width of about 6 mm, have blunt and round tips, and have narrowed bases and claws with the length of about 2 mm, and have multiple veins; stamen are about 5.5 mm long; ovaries are oval, about 4 mm long, style 2, about 4 mm long, stigmas shield-shaped. Chromosome 2n is 34. The flowering period is 6-8 months.

In the Qing Dynasty, the famous plant book of excess syndrome mentioned that bergenia purpurea can cause hematemesis, and in the Qing Dynasty, the Classification of herb properties: resolve phlegm and stop cough. It is indicated for all internal injuries with hematemesis, asthma and stranguria. Modern medical research technology finds that bergenia purpurascens contains bergenin and Arbutin, so that the bergenia purpurascens has antibacterial and anti-inflammatory effects.

Disclosure of Invention

the invention aims to solve the technical problem of providing an application of a bergenia purpurascens extract, in particular to an application in preparing an expression up-regulator of an anti-skin aging gene. The expression up-regulator of the anti-skin aging gene can enhance and support the stability of the skin tissue structure and delay skin aging, and can be used in cosmetics or skin care products.

The invention provides an application of a bergenia purpurascens extract in preparing an expression up-regulator of a skin aging resistance gene.

The skin aging resistant gene can be one or more of COL3A1 gene, FBN1 gene, SIRT1 gene and NID1 gene.

the bergenia tenacissima extract can be a bergenia tenacissima extract which is conventional in the field, can also be a bergenia tenacissima alcohol extract, can also be a bergenia tenacissima C1-C5 alcohol extract, and can also be a bergenia tenacissima alcohol extract; the ethanol can be 70-95% ethanol.

The bergenia mellifera extract can be extract of bergenia mellifera root.

The preparation method of the bergenia purpurascens extract can be a conventional method in the field, and can also comprise the following steps:

(1) Extracting powder of bergenia purpurascens with ethanol, and filtering to obtain filtrate;

(2) Drying the filtrate.

In the step (1), the powder of bergenia purpurascens may be powder of bergenia purpurascens root.

in the step (1), the mesh number of the bergenia purpurascens powder can be 20-60 meshes, and can also be 20 meshes.

In the step (1), the ethanol can be 70-95% ethanol.

In the step (1), the addition amount of the ethanol can be 8-12 times of the volume of the bergenia purpurascens powder, and can also be 10 times.

In the step (1), the extraction temperature can be room temperature, and the room temperature is 20-30 ℃.

In the step (1), the extraction may be ultrasonic extraction. The frequency of the ultrasonic wave can be 400-550W, and can also be 500W. The ultrasonic treatment time can be 25-40min, and can also be 30 min.

In the step (1), the number of times of extraction may be 1 or more, or may be 2.

In step (1), the filtration method may be centrifugation. The speed of centrifugation can be 6000-10000rpm, and can also be 8000 rpm. The centrifugation time can be 8-12 minutes, and can also be 10 minutes.

The preparation of the bergenia purpurascens extract can further comprise the following steps:

(1) Adding 70% ethanol 10 times the volume of the powder of root of bergenia purpurascens into the powder of root of bergenia purpurascens, performing 500W ultrasonic extraction at 20-30 deg.C for 2 times, each for 30min, centrifuging, filtering, and mixing filtrates; the speed of the centrifugation is 8000rpm, and the time of the centrifugation is 10 minutes;

(2) And evaporating the filtrate to dryness.

The concentration of the bergenia purpurascens extract in the expression up-regulator of the skin aging resistant gene can be 8-120 mug/mL, and can also be 10-100 mug/mL.

The concentration of the bergenia purpurascens extract in the expression up-regulator aiming at the FBN1 gene and/or NID1 gene can be 8-120 mug/mL, and can also be 10-100 mug/mL.

The concentration of the bergenia purpurascens extract in the expression up-regulator for COL3A1 and/or SIRT1 genes can be 80-120 mu g/mL, and can also be 100 mu g/mL.

the expression up-regulator of the anti-skin aging gene can be used in cosmetics or skin care products.

The bergenia purpurascens extract can be used in cosmetics or skin care products.

On the basis of the common knowledge in the field, the above preferred conditions can be combined randomly to obtain the preferred embodiments of the invention.

The reagents and starting materials used in the present invention are commercially available.

In the present invention, room temperature means 20 to 30 ℃.

The positive progress effects of the invention are as follows: the application of the bergenia purpurascens extract is particularly to the application of the bergenia purpurascens extract in preparing an expression up-regulator of a skin aging resistant gene, the expression up-regulator of the skin aging resistant gene can enhance and support the stability of a skin tissue structure, delay skin aging, and can be used in cosmetics or skin care products.

drawings

FIG. 1 is a schematic diagram showing the results of promoting COL3A1 gene expression by using 100. mu.g/mL bergenia purpurascens extract.

FIG. 2 is a schematic diagram showing the result of promoting FBN1 gene expression by using a bergenia purpurascens extract of 10. mu.g/mL.

FIG. 3 is a schematic diagram showing the result of promoting FBN1 gene expression by 100. mu.g/mL bergenia purpurascens extract.

FIG. 4 is a schematic diagram of the result of the bergenia purpurascens extract promoting SIRT1 gene expression at 100. mu.g/mL.

FIG. 5 is a graph showing the results of using a bergenia crassifolia extract of 10. mu.g/mL to promote NID1 gene expression.

FIG. 6 is a schematic diagram of NID1 gene expression promotion results of 100 μ g/mL bergenia purpurascens extract.

Detailed Description

The invention is further illustrated by the following examples, which are not intended to limit the scope of the invention. The experimental methods without specifying specific conditions in the following examples were selected according to the conventional methods and conditions, or according to the commercial instructions.