阅读说明：本技术 一种含有枯草芽孢杆菌粉的药物组合物及其应用 (pharmaceutical composition containing bacillus subtilis powder and application thereof ) 是由 王业富 胡坤坤 范宇鹏 李靖 胡定邦 于 2019-07-04 设计创作，主要内容包括：本发明提供了一种含有枯草芽孢杆菌粉的药物组合物及其应用,所述药物组合物按质量百分比包括如下组份：枯草芽孢杆菌粉70-80％、黄连粉1-5％、大枣粉10-20％和土党参粉8％-20％；本发明通过将益生菌与中药相组合,以筛选得到的枯草芽孢杆菌为主要成分,精选中药组分,优化组分配比,各组分协同增效,标本兼治,共同恢复肠道菌群平衡,快速有效止泻；本发明配方简洁高效,止泻作用显著,无毒副作用,并能够增强免疫力,具备广阔的前景。(The invention provides a pharmaceutical composition containing bacillus subtilis powder and application thereof, wherein the pharmaceutical composition comprises the following components in percentage by mass: 70-80% of bacillus subtilis powder, 1-5% of coptis root powder, 10-20% of Chinese date powder and 8-20% of campanumoea root powder; according to the invention, probiotics and traditional Chinese medicines are combined, screened bacillus subtilis is taken as a main component, traditional Chinese medicine components are selected, the component proportion is optimized, the components synergia, both symptoms and root causes are treated, the balance of intestinal flora is recovered, and the diarrhea is quickly and effectively checked; the Chinese medicinal composition has the advantages of simple and efficient formula, remarkable antidiarrheal effect, no toxic or side effect, capability of enhancing immunity and wide prospect.)

1. The pharmaceutical composition containing the bacillus subtilis powder is characterized by comprising the following components in percentage by mass:

70-80% of bacillus subtilis powder

1 to 5 percent of coptis root powder

10 to 20 percent of Chinese date powder

8-20% of campanumoea root powder;

The sum of the percentages of the pharmaceutical composition does not exceed 100%.

2. The pharmaceutical composition according to claim 1, wherein the pharmaceutical composition comprises the following components in percentage by mass:

70 to 75 percent of bacillus subtilis powder

1 to 3 percent of coptis root powder

10 to 15 percent of Chinese date powder

8-15% of campanumoea root powder;

The sum of the percentages of the pharmaceutical composition does not exceed 100%.

3. The pharmaceutical composition according to claim 1 or 2, wherein the bacillus subtilis powder is named as Subtilisin-QK deposited at the national culture collection with the collection number of CCTCCNO: m203078, the preservation date is 7/8/2003.

4. The pharmaceutical composition according to any one of claims 1 to 3, wherein the total number of colonies of Bacillus subtilis is not less than 2 x 1011 cfu/g.

5. a method for preparing the pharmaceutical composition as claimed in any one of claims 1 to 4, wherein the pharmaceutical composition is obtained by removing the core of Zizyphi fructus, pulverizing, sieving with Coptidis rhizoma powder, radix Campanumoeae powder and Bacillus subtilis powder, respectively, and mixing.

6. the method of claim 5, wherein the screened mesh size is 80-100 mesh.

7. The method as claimed in claim 5 or 6, wherein the medicinal composition is obtained by removing the core of the Chinese date, pulverizing, sieving with 80-100 mesh sieve together with Coptidis rhizoma powder, radix Campanumoeae powder and Bacillus subtilis powder, respectively, and mixing.

8. Use of a pharmaceutical composition according to any one of claims 1-4 for the treatment of diarrhea.

9. The use of claim 8, wherein the diarrhea comprises diarrhea caused by an imbalance in intestinal flora.

10. The use according to claim 9, wherein the cause of the imbalance in gut flora comprises antibiotic abuse and/or pathogen infection.

Technical Field

The invention relates to the field of biology, and mainly relates to a pharmaceutical composition containing bacillus subtilis powder and application thereof.

Background

Clinically, diarrhea is generally classified into infectious diarrhea and non-infectious diarrhea, wherein infectious diarrhea accounts for about 80%, and in infectious diarrhea, people are used to treatment with antibiotics. However, due to the massive blind use of antibiotics, beneficial bacteria in the intestinal tract are inhibited, and the microecological balance of the intestinal tract is broken, so that the flora is disordered. The gastrointestinal tract of a healthy person is populated by a wide variety of microorganisms, which are referred to as intestinal flora. The intestinal flora is combined according to a certain proportion, the bacteria are restricted and dependent with each other, an ecological balance is formed on the quality and quantity, once the internal and external environments of the organism change, especially when the broad-spectrum antibiotics are applied for a long time, the sensitive intestinal flora is inhibited, and the bacteria which are not inhibited multiply by the organism, so that the flora imbalance is caused, the normal physiological combination of the sensitive intestinal flora is destroyed, the pathological combination is generated, and the clinical symptoms are called as the intestinal flora imbalance.

The bacillus subtilis is gram-positive aerobic bacteria of the bacillus subtilis, quickly consumes free oxygen in the environment in the growth process, causes hypoxia in intestinal tracts, promotes the growth of beneficial anaerobic bacteria, generates organic acids such as lactic acid and the like, reduces the pH value of the intestinal tracts, and indirectly inhibits the growth of other pathogenic bacteria; in addition, Bacillus subtilis cells synthesize enzymes such as α -amylase, protease, lipase, and cellulase, and act together with digestive enzymes in the body in the digestive tract. The bacillus subtilis spores can be reactivated in intestinal tracts, oxygen in the surrounding environment is consumed in the growth process, the growth of probiotics (lactic acid bacteria, bifidobacteria, clostridium butyricum and the like) in the intestinal tracts is promoted, and meanwhile, conditional pathogenic bacteria such as escherichia coli, salmonella and the like are inhibited. Therefore, the bacillus subtilis probiotics can be used for treating diarrhea caused by dysbacteriosis.

Disclosure of Invention

Aiming at the defects of the prior art and the market demand, the invention provides a pharmaceutical composition containing bacillus subtilis powder and application thereof.

In order to achieve the purpose, the invention adopts the following technical means:

In a first aspect, the invention provides a pharmaceutical composition containing bacillus subtilis powder, which comprises the following components in percentage by mass:

70-80% of bacillus subtilis powder

1 to 5 percent of coptis root powder

10 to 20 percent of Chinese date powder

8-20% of campanumoea root powder;

The sum of the percentages of the pharmaceutical composition does not exceed 100%.

The bacillus subtilis is gram-positive aerobic bacteria of the bacillus subtilis, quickly consumes free oxygen in the environment in the growth process, causes hypoxia in intestinal tracts, promotes the growth of beneficial anaerobic bacteria, generates organic acids such as lactic acid and the like, reduces the pH value of the intestinal tracts, and indirectly inhibits the growth of other pathogenic bacteria. Therefore, the bacillus subtilis probiotics can be used for treating diarrhea caused by dysbacteriosis.

preferably, the pharmaceutical composition comprises the following components in percentage by mass:

70 to 75 percent of bacillus subtilis powder

1 to 3 percent of coptis root powder

10 to 15 percent of Chinese date powder

8-15% of campanumoea root powder;

The sum of the percentages of the pharmaceutical composition does not exceed 100%.

Preferably, the bacillus subtilis powder is named as Subtilisin-QK and is preserved in the national type culture Collection with the preservation number of CCTCCNO: m203078, the preservation date is 11/17/2003.

Preferably, the total number of colonies of Bacillus subtilis is more than or equal to 2 × 1011cfu/g, for example, 2 × 1011cfu/g, 2.5 × 1011cfu/g, 3 × 1011cfu/g, 3.5 × 1011cfu/g or 4 × 1011cfu/g, and when the total number of colonies of Bacillus subtilis is more than the value, the effect in intestinal tract can be fully exerted.

In the invention, an inventor screens a novel bacillus subtilis strain Subtilisin-QK in the long-term scientific research practice process, and preserves the bacillus subtilis strain Subtilisin-QK, combines probiotics and traditional Chinese medicines, takes the screened bacillus subtilis as a main component, carefully selects traditional Chinese medicine components, optimizes the component proportion, realizes synergistic interaction of the components, treats both symptoms and root causes, jointly restores the balance of intestinal flora, and quickly and effectively stops diarrhea; compared with other bacillus subtilis, the strain is found to have better effect on improving diarrhea caused by intestinal dysbacteriosis; the Chinese medicinal composition has the advantages of simple and efficient formula, remarkable antidiarrheal effect, no toxic or side effect, capability of enhancing immunity and wide prospect.

Wherein the mass percentage of the bacillus subtilis is 70-80%, for example, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79% or 80%.

Wherein, the mass percentage of the coptis powder is 1-5%, for example, 1%, 2%, 3%, 4% or 5%.

the coptis powder is a medicine, and is mainly prepared from coptis which is divided into three dry roots and stems of goldthread, coptis deltoidea and coptis yunnanensis of Ranunculaceae, which are respectively called as 'Weilian', 'Yalian' and 'Yunlian', and is mainly produced in Sichuan, Yunnan, Hunan, Hubei and other places, harvested in autumn, removed fibrous root and sediment, dried, has the effects of clearing heat, drying dampness, purging pathogenic fire and removing toxicity, and can resist bacteria, inflammation, ulcer, diarrhea and the like.

The mass percentage of the jujube powder is 10-20%, for example, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19% or 20%.

The Chinese date, also called red date, is rich in protein, fat, sugar, carotene, B vitamins, vitamin C, vitamin P, calcium, phosphorus, iron, cyclic adenosine monophosphate and other nutritional ingredients, has sweet taste and warm nature, and is nontoxic; has the effects of tonifying deficiency and qi, nourishing blood and tranquilization, strengthening spleen and stomach and the like, and has better curative effects on diseases such as chronic hepatitis, liver cirrhosis, anemia, allergic purpura and the like; the Chinese date can moisten heart and lung, relieve cough, nourish five internal organs, treat deficiency, eliminate qi weakness of intestines and stomach, calm middle energizer, nourish spleen, calm stomach qi, dredge nine orifices, assist twelve meridians and the like.

Wherein, the campanumoea powder accounts for 8-20% by mass, for example, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19% or 20%.

Radix Campanumoeae is root of Leopard parva of Campanulaceae, and enters spleen and lung meridians; has the functions of invigorating spleen, benefiting vital energy, invigorating lung and relieving cough, and is mainly used for treating consumptive disease, internal injury, deficiency of vital energy, hypodynamia, palpitation, hyperhidrosis, spleen deficiency, diarrhea and the like.

The invention takes the screened bacillus subtilis strain as a main component, matches the Chinese date powder, the coptis powder and the campanumoea root powder, activates intestinal flora by the bacillus subtilis, inhibits the growth of germs, regulates the spleen and stomach of the intestinal tract by carefully selecting traditional Chinese medicine components, enhances the activity of cells, supplements and promotes each other, and exerts and enhances the antidiarrheal effect together.

The bacillus subtilis Subtilisin-QK can secrete vitamin B2, B6 and nicotinic acid, and according to the immune view, the B-group microorganisms can promote the activities of human macrophages and interferon, activate T, B lymphocytes and enhance the effects of cellular immunity and humoral immunity, and the bacillus subtilis can secrete enzymes such as protease, alpha-amylase and the like and has the combined action with human digestive enzymes in the digestive tract; meanwhile, enzymes generated by bacillus subtilis can further decompose compounds in the Chinese date powder, the coptis powder and the campanumoea powder into small molecular compounds to promote absorption of a human body; further research shows that the growth of the bacillus subtilis can be promoted by adding part of the Chinese date powder, the coptis powder and the campanumoea root powder in the bacillus subtilis culture medium; therefore, the bacillus subtilis, the Chinese date powder, the coptis powder and the campanumoea root powder can play a mutual promotion effect.

In a second aspect, the present invention provides a method for preparing the pharmaceutical composition according to the first aspect, the method comprises removing cores from fructus Jujubae, pulverizing, sieving with Coptidis rhizoma powder, radix Campanumoeae powder and Bacillus subtilis powder, respectively, and mixing to obtain the pharmaceutical composition.

preferably, the mesh size of the screen is 80-100 mesh, for example, 80 mesh, 85 mesh, 90 mesh, 95 mesh or 100 mesh.

Preferably, the machine for mixing is a three-dimensional mixer.

As a preferred technical solution, a method for preparing the pharmaceutical composition according to the first aspect specifically comprises: removing cores of fructus Jujubae, pulverizing, sieving with 80-100 mesh sieve, respectively, and mixing with three-dimensional mixer to obtain the pharmaceutical composition.

In a third aspect, the present invention provides the use of a pharmaceutical composition according to the first aspect for the treatment of diarrhea.

Preferably, the diarrhea comprises diarrhea caused by an imbalance in the intestinal flora.

The gastrointestinal tract of a healthy person is populated by a wide variety of microorganisms, which are referred to as intestinal flora. The intestinal flora is combined according to a certain proportion, the bacteria are restricted and dependent with each other, an ecological balance is formed on the quality and quantity, once the internal and external environments of the organism change, especially when the broad-spectrum antibiotics are applied for a long time, the sensitive intestinal flora is inhibited, and the bacteria which are not inhibited multiply by the organism, so that the flora imbalance is caused, the normal physiological combination of the sensitive intestinal flora is destroyed, the pathological combination is generated, and the clinical symptoms are called as the intestinal flora imbalance.

Preferably, the cause of the imbalance in the intestinal flora comprises antibiotic abuse and/or a pathogenic infection.

In a fourth aspect, the present invention provides a medicament comprising a pharmaceutical composition according to the first aspect.

Preferably, the medicament further comprises any one or a combination of at least two of a pharmaceutically acceptable carrier, excipient or diluent.

Compared with the prior art, the invention has the following advantages:

(1) The specific bacillus subtilis obtained by screening is selected, the traditional Chinese medicine components are selected, the component formula is optimized, the formula is simple and efficient, the components are synergistic, the intestinal flora balance is recovered together, both the symptoms and root causes are treated, the diarrhea is quickly and effectively stopped, the dampness and the spleen are eliminated, the diarrhea is stopped, the qi is benefited, the intestinal injury is small, the taking is convenient, the diarrhea stopping effect is obvious, no toxic or side effect is caused, the safety and the reliability are realized, the immunity can be enhanced, and the wide prospect is realized;

(2) The method provided by the invention is simple and easy to learn, can be obtained by crushing, sieving and mixing the formula components, is simple and convenient to operate, and is easy to popularize and operate.

drawings

FIG. 1 is a BLAST result chart of the strain of the present invention.

Detailed Description

To further illustrate the technical means and effects of the present invention, the following preferred embodiments of the present invention are further described, but the present invention is not limited to the scope of the embodiments.