阅读说明：本技术 一种光聚可颜色自反馈硬度分布的凝胶及制备方法、应用 (Photopolymerisable gel capable of realizing color self-feedback hardness distribution, preparation method and application ) 是由 顾忠泽 李森 杜鑫 于 2019-09-25 设计创作，主要内容包括：本发明公开了一种光聚可颜色自反馈硬度分布的凝胶及其制备方法,所述凝胶用于细胞支架,凝胶的制备步骤如下：配制预聚液；将预聚液灌入两块平行放置的玻璃片中,用365 nm紫外光照射使之聚合；过程中可用254 nm和365 nm紫外光对凝胶的力学性质进行调控,获得力学性质区域化分布的细胞支架。改性后的细胞支架的硬度及其分布会以颜色的方式显现出来,通过拍摄照片原位地读取支架上的硬度分布。该方法应用于多种细胞培养系统,实时的控制微环境的性质,原位引导其上细胞的行为。不打开芯片且不外加检测设备的情况下,通过颜色分析直接获取调控后的细胞支架的力学参数,及细胞行为和支架性质间的关系,具有很大的应用潜力。(the invention discloses a light polymerization gel with color self-feedback hardness distribution and a preparation method thereof, wherein the gel is used for a cell scaffold, and the preparation steps of the gel are as follows: preparing a pre-polymerization solution; pouring the prepolymerization solution into two glass sheets which are arranged in parallel, and irradiating by using 365nm ultraviolet light to polymerize the solution; in the process, 254nm and 365nm ultraviolet light can be used for regulating and controlling the mechanical property of the gel to obtain the cell scaffold with regionalized distribution of the mechanical property. The hardness and distribution of the modified cell scaffold will be visualized in color, and the hardness distribution on the scaffold is read in situ by taking a photograph. The method is applied to various cell culture systems, controls the properties of the microenvironment in real time, and guides the behavior of cells on the microenvironment in situ. Under the conditions of not opening the chip and not adding detection equipment, the mechanical parameters of the regulated cell scaffold and the relation between the cell behavior and the scaffold property are directly obtained through color analysis, and the method has great application potential.)

1. A photopolymerisable, colorable, self-feedbackable hardness-distributing gel characterized by: the gel visually obtains the hardness distribution through color change.

2. use of a photopolymerisable, colour-self-feedback hardness-distributing gel, characterized in that: the gel with the photopolymerisable color self-feedback hardness distribution is used for a cell culture scaffold.

3. A preparation method of gel with photopolymerisable color and self-feedback hardness distribution is characterized by comprising the following steps: the method comprises the following steps:

step 1) preparing gel pre-polymerization liquid; the specific method comprises the following steps: uniformly mixing 8-15 wt% of acrylamide, 0.08-0.45 wt% of methylene bisacrylamide/polyethylene glycol diacrylate, 0.08-0.45 wt% of photoinitiator and 26-34 vol% of silicon dioxide particles as raw materials, and water as a solvent to prepare a pre-polymerization solution;

Step 2) pouring the pre-polymerization solution into a slide clamp on the basis of the pre-polymerization solution prepared in the step 1), wherein the thickness of a gasket is 10-125 mu m; polymerizing to form a film under the condition of ultraviolet illumination to prepare gel films with different colors and hardness;

and 3) on the basis of the gel film prepared in the step 2), placing a gray mask on the surface of the film, and obtaining the gel with regionalized distribution characteristics of hardness and color under 365nm ultraviolet illumination.

4. the method of claim 3, wherein: subjecting the prepolymerization solution obtained in the step 1) to ultrasonic treatment for 5 minutes.

5. the method of claim 3, wherein: the ultraviolet illumination condition in the step 2) refers to that the wavelength of the ultraviolet light is 365nm, and the illumination time is increased in a gradient manner from 10s to 30 min.

6. The method of claim 3, wherein: the ultraviolet illumination condition in the step 2) refers to ultraviolet light with the wavelength of 254nm, the illumination intensity is 0.1-10mW/cm & lt-2 & gt, and the illumination time is 10s-30 min.

7. A preparation method of gel with adjustable light polymerization and color self-feedback hardness distribution is characterized in that: the method comprises the following steps:

Step 1) preparing gel pre-polymerization liquid; the specific method comprises the following steps: uniformly mixing 8-15 wt% of acrylamide, 0.08-0.45 wt% of methylene bisacrylamide/polyethylene glycol diacrylate, 0.08-0.45 wt% of photoinitiator, 26-34 vol% of silicon dioxide particles and 7-acryloyloxy amino-4-methylcoumarin (2-4 wt%) as raw materials, and DMSO as a solvent to prepare a pre-polymerization solution;

Step 2) on the basis of the pre-polymerization solution prepared in the step 1), pouring the pre-polymerization solution into a slide clamp, wherein the thickness of a gasket is 10-125 mu m, and irradiating by using ultraviolet light to prepare a light-adjustable intelligent gel film;

And 3) irradiating and partially depolymerizing the part by 254nm ultraviolet light through a gray mask or different illumination time on the basis of the intelligent gel film prepared in the step 2) to obtain the anisotropic intelligent gel films with different hardness.

8. The method of claim 7, wherein: subjecting the prepolymerization solution obtained in the step 1) to ultrasonic treatment for 5 minutes.

Technical Field

The invention relates to an intelligent cell scaffold for microenvironment in-situ regulation and sensing in an organ chip and a preparation method thereof, belongs to the field of biological materials, and particularly relates to a gel with photopolymerisable color and self-feedback hardness distribution, a preparation method and application thereof.

Background

an organ chip is a new technology for realizing the function of simulating human organs by three-dimensional culture of cells in an in-vitro chip. The organ chip has wide application prospect in the fields of new drug research and development, disease models, personalized medicine, aerospace medicine and the like. In 2016, organ chips were listed as "ten new technologies" by the Darwos economic forum. One of the most challenging links in drug development is how to test the effectiveness and safety of drugs. Cell and animal experiments are two experiment platforms widely used in the current drug research and development and evaluation processes, the former is difficult to simulate the human body microenvironment, and the latter is complex, expensive, time-consuming and has ethical debate. Therefore, economical, efficient animal or clinical replacement experiments are necessary. Organ chips are a new experimental method for drug evaluation which has been proposed in recent years.

The organ chip is a three-dimensional cell culture system based on a microfluid chip and mainly comprises a cover plate, a base plate, a flow channel and a cell culture area. The cell culture zone consists of cells, cell microenvironment, detection system and other parts. Wherein the microenvironment is a very important component thereof, and the microenvironment is mainly composed of the cell scaffold material and the culture medium. The cell scaffold can simulate extracellular matrix (ECM), can control cell behaviors by regulating the function of the scaffold, and becomes a key element in the fields of tissue engineering, regenerative medicine, drug screening, organ chips and the like. Among them, hydrogels having superior characteristics of easy manufacturing, high water content and variable physicochemical properties are used to mimic the properties of ECM, which has attracted much attention.

The most important challenge in the aspects of regulation and detection of the current organ chip is that the organ chip is an enclosed system after being assembled, and the microenvironment inside the organ chip cannot be regulated or detected through an enclosed cover plate or a substrate by a conventional detection means. In order to realize detection, the interface of the detection system needs to be designed on the chip in advance, which increases the complexity of the chip on one hand, and on the other hand, the spatial distribution of each parameter in the microenvironment is still difficult to detect in this way.

Disclosure of Invention

The technical problem is as follows: aiming at the defects of the prior art, the invention provides an intelligent cell scaffold with in-situ regulation and sensing functions and a preparation method thereof, wherein the intelligent cell scaffold can be used in an organ chip. The intelligent cell scaffold prepared by the method can adjust the mechanical property of the intelligent cell scaffold in a closed chip through light control, so that a cell culture area in the chip can generate an anisotropic microenvironment in real time, and different cell forms can be obtained. In addition, the mechanical property and the cell growth state of the bracket can be directly obtained through the color of the bracket under the condition of not opening the chip and not adding detection equipment, so that the effect of the cells and the bracket is easy to observe and study.

The technical scheme is as follows: in order to realize the purpose, the invention provides a gel with photopolymerisable color self-feedback hardness distribution, a preparation method and application thereof.

The invention discloses a gel with photopolymerisable color and self-feedback hardness distribution; the gel visually obtains the hardness distribution through color change. The gel can visually obtain the hardness distribution through color change, and has wide application range and simple operation. The gel can directly obtain the hardness distribution of each position of the gel through color change without additional detection equipment.

The invention discloses application of a gel with photopolymerisable color and self-feedback hardness distribution, and the gel is used for a cell scaffold. The support can realize in-situ regulation and sensing of cell growth environment.

the invention discloses a preparation method of a gel with photopolymerisable color and self-feedback hardness distribution, which comprises the following steps:

1) Preparing gel pre-polymerization liquid; the specific method comprises the following steps: taking acrylamide (8-15 wt%), methylene bisacrylamide (Bis)/polyethylene glycol diacrylate (PEGDA700) (0.08-0.45 wt%), photoinitiator (2959) (0.08-0.45 wt%), monodisperse silicon dioxide particles (26-34 vol%) and the like as raw materials, taking water as a solvent, and ultrasonically mixing uniformly to prepare a pre-polymerization solution;

2) On the basis of the pre-polymerization solution prepared in the step 1), pouring the pre-polymerization solution into a slide clamp (with the thickness of a gasket being 10-125 mu m), polymerizing to form a film under the condition of 365nm ultraviolet illumination, and gradually increasing the illumination time from 10s to 30min to prepare gel films with different colors and hardness;

3) On the basis of the gel film prepared in the step 2), a gray mask is placed on the surface of the film, and the gel with regionalized distribution characteristics of hardness and color is obtained under 365nm ultraviolet illumination.

The other technical scheme of the preparation method of the invention is as follows: step 1) preparing gel pre-polymerization liquid; the specific method comprises the following steps: acrylamide (8-15 wt%), Bis/PEGDA700(0.08-0.45 wt%), photoinitiator (2959) (0.08-0.45 wt%), silicon dioxide particles (26-34 vol%), 7-acryloyloxy amino-4-methylcoumarin (2-4 wt%) and the like are used as raw materials, DMSO is used as a solvent, and the raw materials are uniformly mixed to prepare a pre-polymerization solution;

step 2) on the basis of the pre-polymerization solution prepared in the step 1), filling the pre-polymerization solution into a slide clamp (with the thickness of a gasket being 25-125 mu m), and irradiating by using 365nm ultraviolet light to prepare a light-adjustable intelligent gel film;

And 3) on the basis of the intelligent gel film prepared in the step 2), placing the film in a pre-designed organ chip, and irradiating and partially depolymerizing the film by 254nm ultraviolet light through a gray mask or different illumination time when the mechanical property of the film needs to be changed to obtain the anisotropic intelligent gel films with different hardness. The dicoumarin is depolymerized by 254nm ultraviolet light, the hardness of the gel is regulated, and the intelligent gel film with regionally distributed hardness can be obtained by carrying out patterned depolymerization on the gel by using a gray mask. For the depolymerized film, ultraviolet irradiation at 365nm can be used for recombining coumarin, so that the hardness of the gel is improved, and the hardness change is also shown in a color change mode.

On the basis of the steps 3) and 6), when the regulated and controlled mechanical property distribution of the gel needs to be obtained, a camera is used for imaging the gel, and the hardness distribution of the gel can be obtained by analyzing the hues of different areas on the photo.

The gel can directly obtain the hardness distribution of each position of the gel through color change without additional detection equipment. The intelligent gel capable of optically controlling in-situ regulation and sensing is used as a cell culture support, the hardness of the support can be regulated and controlled in situ through photochemical reaction, so that the attachment and growth forms of cells on the surface of the support are different, and the distribution information of the hardness of the support can be directly obtained without an external instrument. The application of the intelligent bionic cell scaffold capable of optically controlling in-situ regulation and sensing in an organ chip can regulate and control the internal cell scaffold in situ through ultraviolet irradiation under the condition of not opening the chip, so that the mechanical property of the internal cell scaffold generates in-situ and area-controllable changes, the cell behavior in the chip is influenced, and the cell morphology can be directly observed in the chip and color data can be read to obtain the information of the relationship between the cell growth state and the substrate property.

Wherein:

The further technical scheme of the invention is as follows: subjecting the prepolymerization solution obtained in the step 1) to ultrasonic treatment for 5 minutes.

The further technical scheme of the invention is as follows: the composition of the prepolymerization solution in the step 1) comprises but is not limited to substances such as acrylamide, Bis/PEGDA700, a photoinitiator (2959), silica particles, coumarin acrylate, water and DMSO (dimethyl sulfoxide).

The further technical scheme of the invention is as follows: the ultraviolet illumination condition in the step 2) refers to that the wavelength of the ultraviolet light is 365nm, and the illumination time is increased in a gradient manner from 10s to 30 min.

The further technical scheme of the invention is as follows: the ultraviolet illumination condition in the step 2) refers to ultraviolet light with the wavelength of 254nm, the illumination intensity is 0.1-10mW/cm & lt-2 & gt, and the illumination time is 10s-30 min.

Has the advantages that: compared with the prior art, the invention has the following advantages:

1. the invention provides an intelligent cell scaffold for microenvironment in-situ regulation and sensing in an organ chip and a preparation method thereof. The method can be applied to cell culture scaffolds in various organ chips.

2. According to the intelligent cell scaffold for in-situ microenvironment regulation and sensing in the organ chip, disclosed by the invention, the mechanical property of the scaffold can be regulated in a closed chip through light control, so that a cell culture area in the chip can generate an anisotropic microenvironment in real time, and different cell forms can be obtained.

3. According to the intelligent cell scaffold for in-situ microenvironment regulation and sensing in the organ chip, provided by the invention, the mechanical property and the cell growth state of the scaffold can be directly obtained through colors under the conditions that the chip is not opened and detection equipment is not additionally arranged.

4. Firstly, preparing a pre-polymerization solution according to a pre-calculated formula; then, the prepolymer solution was poured into two glass plates placed in parallel and polymerized by irradiation with 365nm ultraviolet light. In the using process, the mechanical property of the gel can be regulated and controlled by 254nm and 365nm ultraviolet light, and the cytoskeleton with regionalized distribution of the mechanical property can be obtained by masking or controlling the local illumination time. Meanwhile, the hardness and the distribution of the modified cell scaffold can be displayed in a color mode, and the hardness distribution on the scaffold can be read in situ by taking a picture. The method can be applied to various cell culture systems, such as cell culture plates, microarrays and microfluidic organ chips, and can regulate the mechanical property of the bracket by light control in a closed environment, control the property of a microenvironment in real time and guide the behavior of cells on the bracket in situ. In addition, the mechanical parameters of the regulated cell scaffold and the relation between the cell behavior and the scaffold property can be directly obtained through color analysis under the conditions that the chip is not opened and detection equipment is not additionally arranged, and the method has great application potential.

drawings

FIG. 1 is a diagram of color change and mechanical property change of an intelligent support provided by the invention under the regulation and control of ultraviolet light;

FIG. 2 is a diagram showing the use of the cell scaffold of the present invention in an organ chip;

FIG. 3 is a photograph of the cultured cells on the scaffold of the chip of FIG. 2 observed under a microscope;

FIG. 4 is a graph comparing the calculated elastic modulus fit value from the hue distribution of the gel with the elastic modulus distribution obtained by an indenter.

Detailed Description

the present invention will be further illustrated with reference to the accompanying drawings and specific examples, which are carried out on the premise of the technical solution of the present invention, and it should be understood that these examples are only for illustrating the present invention and are not intended to limit the scope of the present invention.