阅读说明：本技术 一种桔梗提取物的制备方法 (Preparation method of platycodon grandiflorum extract ) 是由 杨成东 于 2018-05-30 设计创作，主要内容包括：本发明涉及一种桔梗提取物的制备方法,具体涉及一种采用超声-高压破壁法提取桔梗提取物的方法。其处理步骤包括：把桔梗根清洗,在带排风电烘箱干燥处理后,粉碎,加入原料2~3倍的5~10%的乙醇混合成悬浊液,将物料置于超声波装置中进行超声预处理5~15min,将经预处理的溶液置于超高压容器内,施加压力400Pa,在此下保持一定时间,然后泄除压力。从高压容器内取出处理过的溶液,过滤,得浓缩液,干燥,获得桔梗提取物。本发明具有快速、溶剂使用量少、提取完全等优点。(the invention relates to a preparation method of a platycodon extract, in particular to a method for extracting the platycodon extract by adopting an ultrasonic-high pressure wall breaking method.)

1. A preparation method of a platycodon extract is characterized by comprising the following steps:

a. Cleaning radix Platycodi root, drying in electric oven with air exhaust, pulverizing, sieving with 200 mesh sieve, and precisely weighing 30 mg;

b. Putting the materials in an ultrasonic device, adding 5 ~ 10% ethanol solution 2 ~ 3 times of the raw materials, and performing ultrasonic extraction for 5 ~ 15min to obtain a pretreatment solution;

c. The pretreated solution was placed in an ultra-high pressure vessel, pressure 400Pa was applied, the pressure was maintained for a certain time, and then the pressure was released. Concentrating, and drying to obtain radix Platycodi extract.

2. The method for preparing platycodon grandiflorum extract according to claim 1, wherein the power of the air-exhausting electric oven in the step a is 500W.

3. The method for preparing Platycodon grandiflorum extract according to claim 1, wherein the ultrasonic extraction in step b is performed at a frequency of 50KHz and an ultrasonic temperature of 40 ~ 60 ℃.

4. The method of claim 1, wherein the power of the ultra-high pressure vessel in step c is 3000W.

Technical Field

the invention relates to a preparation method of a platycodon extract, belonging to the field of extraction of effective components of traditional Chinese medicines.

Background

Platycodon grandiflorum, namely burley flowers, small bell flowers and monkshood flowers, is a perennial herbaceous plant, has a stem height of 20-120 cm, is generally hairless, is occasionally densely covered with short hairs, is not branched, and has few upper branches. The leaves are all rotated to be completely and alternately generated, no stem or extremely short stem exists, the leaves are oval, oval-shaped to be needle-shaped, the leaves are oval or oval-shaped to be needle-shaped, and the flowers are dark blue or dark purple white and can be used as ornamental flowers; the root of the Chinese medicinal herb can be used as a medicine, has the effects of relieving cough, eliminating phlegm, dispersing lung qi, expelling pus and the like, and is a common Chinese medicine. It is usually salted in northeast China and used for making pickles in the fresh peninsula, and the local ballad balloon flower ballad is described in.

the platycodon root extract is mainly used for ventilating the lung, relieving sore throat, eliminating phlegm and expelling pus. Can be used for treating cough with excessive phlegm, chest distress, pharyngalgia, hoarseness, lung carbuncle, suppuration, and pyocutaneous disease with pus formation.

At present, the main extraction methods of the platycodon extract include a solvent extraction method and an ultrasonic extraction method. The solvent extraction method has the highest efficiency by water extraction, but has the defects of more extraction impurities, difficult operation of filtering and concentrating the extracting solution, time consumption and the like; the ultrasonic extraction method is energy-saving, greatly shortens the time, and has low stability.

The high pressure extraction is to use ultra-high pressure to make radix Platycodi extract bear static pressure, i.e. bear equal pressure in normal direction. Thus, the cell wall of the Platycodon grandiflorum extract is compressed and deformed. The cell wall is broken when the pressure is higher than a certain pressure. The solvent is contacted with the contents of the platycodon extract. To extract the effective components in the tissue. The effective components of the plants are extracted by a high pressure method, the materials can be directly placed in a high pressure container, and the material and an extraction solvent are in an ultrahigh pressure device, the cell walls of the materials are broken under the action of pressure, so that the effective components are dissolved out, and the aims of quickly and completely extracting the effective components of the plants are fulfilled.

And the cavitation effect and the mechanical effect of the ultrasonic wave are combined, so that the effective components in the cells are released to be directly contacted with the solvent and dissolved in the solvent, and the extraction rate of the effective components is improved.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and aims to provide a method for preparing the platycodon grandiflorum extract, which has high efficiency and selectivity, is simple and convenient to operate, has high yield and few byproducts and can be industrially realized.

The technical scheme of the invention is carried out according to the following steps:

a. Cleaning radix Platycodi root, drying in electric oven with air exhaust, pulverizing, sieving with 200 mesh sieve, and precisely weighing 30 mg;

b. putting the materials in an ultrasonic device, adding 5 ~ 10% ethanol solution 2 ~ 3 times of the raw materials, and performing ultrasonic extraction for 5 ~ 15min to obtain a pretreated solution;

c. the pretreated solution was placed in an ultra-high pressure vessel, pressure 400Pa was applied, the pressure was maintained for a certain time, and then the pressure was released. Concentrating, and drying to obtain radix Platycodi extract.

The power of the air exhaust electric oven in the step a is 500W.

The ultrasonic extraction conditions in the step b are that the frequency is 50KHz, and the ultrasonic temperature is 40 ~ 60 ℃.

The pressure of the ultrahigh pressure vessel in the step c is 100-1000 Pa.

The invention has the advantages that:

1. The invention adopts the ultrasonic-ultrahigh pressure treatment of the raw materials, realizes the acceleration of cell disruption, has high extraction rate and short extraction time, and can effectively avoid the damage of the components of the platycodon grandiflorum extract.

2. The invention adopts an ultrasonic-high pressure wall breaking method to extract the platycodon extract, and has the advantages of good taste, low pesticide residue, low heavy metal and 100 percent cold dissolution of water solubility.

The present invention will be further described with reference to specific embodiments, but the scope of the invention as claimed is not limited to the following embodiments.

The specific implementation mode is as follows: