阅读说明：本技术 一种复方砂生槐消毒剂及其制备方法 (Compound sophora moorcroftianain disinfectant and preparation method thereof ) 是由 袁瑞瑛 卓玛东智 郭桅 于 2019-10-24 设计创作，主要内容包括：本发明公开了一种复方砂生槐消毒剂及其制备方法,该消毒剂包括以下按重量份计的原料：砂生槐地上部分10-70份、拉萨小檗10-50份和结血蒿10-50份。制备方法为将砂生槐地上部分、拉萨小檗和结血蒿药材处理后,加乙醇回流提取,提取液合并,减压回收乙醇,得浓缩液,浓缩液水沉过滤后,滤液减压浓缩,得提取物；提取物加入异丙醇,混合均匀,加入蒸馏水,混合均匀,即得复方砂生槐消毒剂。本发明所提供的消毒剂将砂生槐地上部分、拉萨小檗和结血蒿配合使用,杀菌消毒作用明显,且原料均来源于天然,安全性高。(The invention discloses a compound sophora moorcroftianain disinfectant and a preparation method thereof, wherein the disinfectant comprises the following raw materials in parts by weight: 10-70 parts of overground parts of sophora moorcroftiana, 10-50 parts of rassa barberry and 10-50 parts of artemisia integrifolia. The preparation method comprises processing aerial parts of Sophora Moorcroftiana, Berberis Lasa and herba Artemisiae Annuae, extracting with ethanol under reflux, mixing extractive solutions, recovering ethanol under reduced pressure to obtain concentrated solution, precipitating with water, filtering, and concentrating the filtrate under reduced pressure to obtain extract; adding isopropanol into the extract, mixing, adding distilled water, and mixing to obtain compound Sophora Moorcroftiana disinfectant. The disinfectant provided by the invention is prepared by matching overground parts of sophora moorcroftiana, the Berberis rassa and the artemisia scoparia, has obvious sterilization and disinfection effects, is prepared from natural raw materials and has high safety.)

1. the compound sophora moorcroftianain disinfectant is characterized by comprising the following raw materials in parts by weight:

10-70 parts of overground parts of sophora moorcroftiana, 10-50 parts of rassa barberry and 10-50 parts of artemisia integrifolia; wherein aerial parts of Sophora Moorcroftiana contain no fruit.

2. The compound sophora moorcroftianain disinfectant as claimed in claim 1, comprising the following raw materials by weight:

20-60 parts of overground parts of sophora moorcroftiana, 20-40 parts of rassa barberry and 20-40 parts of artemisia integrifolia; wherein aerial parts of Sophora Moorcroftiana contain no fruit.

3. The compound sophora moorcroftianain disinfectant as claimed in claim 1, comprising the following raw materials by weight:

60 parts of overground parts of sophora moorcroftiana, 20 parts of rassa barberry and 20 parts of artemisia capillaris; wherein aerial parts of Sophora Moorcroftiana contain no fruit.

4. The method for preparing the compound sophora moorcroftianain disinfectant as claimed in any one of claims 1 to 3, characterized by comprising the following steps:

S1, taking dried overground parts of sophora moorcroftiana, the rassa barberry and the artemisia integrifolia medicinal materials, crushing and sieving to obtain processed medicinal materials;

S2, adding 30-80 vt% ethanol into the processed medicinal materials obtained in the step S1, performing reflux extraction for 1-3 times, extracting for 1-3 hours each time, combining filtrates, and concentrating under reduced pressure to obtain a concentrated solution;

S3, adding water into the concentrated solution obtained in the step S2, uniformly stirring, standing for 12-24h at 0-5 ℃, filtering, and concentrating under reduced pressure to obtain a compound sophora moorcroftianain disinfectant extract;

S4, adding isopropanol into the compound sophora moorcroftianain disinfectant extract obtained in the step S3, and uniformly mixing to obtain a mixture;

And S5, adding water into the mixture obtained in the step S4, and uniformly mixing to obtain the catalyst.

5. The method for preparing the compound sophora moorcroftianain disinfectant as claimed in claim 4, wherein: the volume-mass ratio of the ethanol in the step S2 to the processed medicinal material obtained in the step S1 is 5-8mL:1 g.

6. the method for preparing the compound sophora moorcroftianain disinfectant as claimed in claim 4, wherein: and concentrating the solution in the step S2 under reduced pressure at 60-80 ℃ to obtain a concentrated solution.

7. The method for preparing the compound sophora moorcroftianain disinfectant as claimed in claim 4, wherein: the volume-mass ratio of the water in the step S3 to the concentrated solution obtained in the step S2 is 3-6mL:1 g.

8. The method for preparing the compound sophora moorcroftianain disinfectant as claimed in claim 4, wherein: and in the step S3, concentrating under reduced pressure at 55-75 ℃ to obtain a concentrated solution.

9. the method for preparing the compound sophora moorcroftianain disinfectant as claimed in claim 4, wherein: the isopropanol content of the mixture in the step S4 is 1-5 wt%.

10. The method for preparing the compound sophora moorcroftianain disinfectant as claimed in claim 4, wherein: the volume-mass ratio of the water in the S5 step to the mixture obtained in the S4 step is 2-5mL:1 g.

Technical Field

the invention belongs to the technical field of disinfectants, and particularly relates to a compound sophora moorcroftianain disinfectant and a preparation method thereof.

Background

The disinfectant is mainly used for killing pathogenic microorganisms on a transmission medium so as to meet the requirement of harmlessness. Currently, the use of disinfectant in daily life is becoming more and more widespread, among which the most used are chemical disinfectants, such as: the disinfectant is an oxidation disinfectant, an aldehyde disinfectant, a phenol disinfectant and the like, and although the disinfectant has an obvious disinfection effect, the disinfectant has certain toxic and side effects on a human body and also has certain pollution on the environment. Traditional medicines such as traditional Chinese medicines and Tibetan medicines have the advantages of good sterilization and disinfection effects and no toxic or side effect. The traditional medicines are matched according to the corresponding compatibility principle, the sterilization and disinfection effects of the traditional medicines are often unexpected, but how to find the proper compatible medicines and determine the optimal medicine proportion is one of the hot spots and difficulties in the research of the traditional medicine disinfectants.

disclosure of Invention

The invention aims to: aiming at the problems in the prior art, the compound sophora moorcroftianain disinfectant and the preparation method thereof are provided, the compound sophora moorcroftianain disinfectant is prepared by the overground part of sophora moorcroftianain, the berberis rassa and the artemisia capillaris, the three medicines are extracted, refined and combined into a whole, and the external disinfectant with low toxicity, safety, effectiveness and small side effect is combined.

The technical scheme adopted by the invention is as follows:

A compound sophora moorcroftianain disinfectant comprises the following raw materials in parts by weight:

10-70 parts of overground parts of sophora moorcroftiana, 10-50 parts of rassa barberry and 10-50 parts of artemisia integrifolia; wherein aerial parts of Sophora Moorcroftiana contain no fruit.

Further, the feed comprises the following raw materials in parts by weight:

20-60 parts of overground parts of sophora moorcroftiana, 20-40 parts of rassa barberry and 20-40 parts of artemisia integrifolia; wherein aerial parts of Sophora Moorcroftiana contain no fruit.

Further, the feed comprises the following raw materials in parts by weight:

60 parts of overground parts of sophora moorcroftiana, 20 parts of rassa barberry and 20 parts of artemisia capillaris; wherein aerial parts of Sophora Moorcroftiana contain no fruit.

The preparation method of the compound sophora moorcroftianain disinfectant comprises the following steps:

s1, taking dried overground parts of sophora moorcroftiana, the rassa barberry and the artemisia integrifolia medicinal materials, crushing and sieving to obtain processed medicinal materials;

S2, adding 30-80 vt% ethanol into the processed medicinal materials obtained in the step S1, performing reflux extraction for 1-3 times, extracting for 1-3 hours each time, combining filtrates, and concentrating under reduced pressure to obtain a concentrated solution;

S3, adding water into the concentrated solution obtained in the step S2, uniformly stirring, standing for 12-24h at 0-5 ℃, filtering, and concentrating under reduced pressure to obtain a compound sophora moorcroftianain disinfectant extract;

S4, adding isopropanol into the compound sophora moorcroftianain disinfectant extract obtained in the step S3, and uniformly mixing to obtain a mixture;

and S5, adding water into the mixture obtained in the step S4, and uniformly mixing to obtain the catalyst.

sophora moozeroftiana (Sophora moozeroftiana) is a plant of Sophora genus of Leguminosae, the Tibetan medicine is called thrips, is a characteristic plant of Tibet, is mainly distributed in Lasa, south of the mountain and the Japanese Kashi regions of Tibet along 2800-4400m mountain slope irrigation clusters of Yaluzang Bujiang, Lasa river, Nichu river and the like, and is often formed into large communities in the Yaluzang Bujiang river valley. The Sophora Moorcroftiana seed is a common medicine for Tibetan, has effects of clearing heat, promoting diuresis, dispelling pathogenic wind and killing parasites, and can be used for treating enteritis, dysentery, damp-heat jaundice, pain swelling sore toxin, skin scabies and tinea, etc. Although the yield of the overground parts such as the leaves and the stems of the sophora moorcroftianain is high and the collection is convenient, the chemical components, the drug effect and the like of the overground parts have no research data. According to the invention, researches show that the overground part of the sophora moorcroftiana mainly contains alkaloid components such as matrine, oxymatrine, sophocarpine and the like, has a strong disinfection effect, and can be applied to the fields of medicines, biological pesticides and the like. If the overground part of the sophora moorcroftianain is further developed, the application range of the sophora moorcroftianain can be effectively enlarged, and the problems of waste of the overground part and the like are reduced.

the Berberis Lassa (Berberis hemsleyana Ahrendt) is a Berberis plant in the berberidaceae family, is mainly distributed in a hillside bush of 4400 meters above sea level 3660-.

artemisia Selengensis (Artemisia vestita Wall) is derived from Artemisia capillaris of Artemisia of Compositae, and aerial part of the Artemisia Selengensis is one of common Tibetan medicines in Tibet region. The medicine is pungent, bitter and cold in nature, and has the effects of clearing away heat and toxic materials, killing parasites and promoting diuresis. The blue colored glaze records that the medicine has the efficacies of resisting bacteria and removing toxicity and treating lumps and sores and ulcers. The Chinese patent of Jingzhu materia medica records that the medicine has the functions of diminishing inflammation and treating insect diseases.

According to the Tibetan medicine formula idea and the modern research result of medicines, the overground part of the sophora moorcroftiana is taken as a monarch medicine, the rassa barberry and the artemisia desmodium are taken as ministerial medicines, and the three medicines are used in a matched manner, so that a clear synergistic and enhanced effect can be achieved, and the sterilization and disinfection effects can be exerted together. Experimental results show that the compound disinfectant prepared by combining the overground part of sophora moorcroftiana, the Berberis rassa and the Artemisia integrifolia has good bactericidal effect and has killing effects on Candida albicans, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Different from the traditional Tibetan medicine using method, the invention develops a new way on the basis of the original efficacy, develops new medicines, extracts and refines the three medicines into a whole, and combines the three medicines into a preparation of the external disinfectant with low toxicity, safety, effectiveness and small side effect.

Further, the volume-to-mass ratio of the ethanol in the step S2 to the processed medicinal material obtained in the step S1 is 5-8mL:1 g.

Further, in the step S2, the concentrated solution is obtained by vacuum concentration at 60-80 ℃.

Furthermore, the volume-to-mass ratio of the water in the step S3 to the concentrated solution obtained in the step S2 is 3-6mL:1 g.

Further, in the step S3, the concentrated solution is obtained by concentrating under reduced pressure at 55-75 ℃.

Further, the isopropanol content of the mixture in the step of S4 is 1-5 wt%.

Further, the volume-to-mass ratio of the water in the step S5 to the mixture obtained in the step S4 is 2-5mL:1 g.

in summary, due to the adoption of the technical scheme, the invention has the beneficial effects that:

1. According to the traditional Tibetan medicine theory, the modern scientific and technical method and means are combined, the overground part of the sophora moorcroftiana, the berberis rassa and the artemisia rupestris are determined to be compatible for use by looking up literature data and carrying out a large number of experimental optimizations, and the sophora moorcroftiana has remarkable disinfection and bacteriostasis effects;

2. The invention combines the overground part of the sophora moorcroftianain with the rassa berberis and the artemisia capillaris, and the three are used in a matching way, thereby playing a definite synergistic enhancement role and playing a role in sterilization and disinfection together;

3. the disinfectant disclosed by the invention has a killing effect on candida albicans, staphylococcus aureus, escherichia coli and pseudomonas aeruginosa, and the sterilization rate is up to more than 97%;

4. the invention has scientific formula, clear effect, natural raw materials, safety superior to that of the general chemical disinfection products sold on the market, and outstanding advantages of low toxicity, safety, effectiveness and small side effect.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the detailed description and specific examples, while indicating the preferred embodiment of the invention, are intended for purposes of illustration only and are not intended to limit the scope of the invention.

thus, the following detailed description of the embodiments of the present invention is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without making any creative effort, shall fall within the protection scope of the present invention.

It is noted that relational terms such as "first" and "second," and the like, may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.

The features and properties of the present invention are described in further detail below with reference to examples.