阅读说明：本技术 一种适于甘薯种质资源盆栽的土壤基质及其筛选实验方法 (Soil matrix suitable for sweet potato germplasm resource pot culture and screening experiment method thereof ) 是由 杨义伶 姚肖健 张雄坚 姚祝芳 黄立飞 房伯平 于 2021-08-09 设计创作，主要内容包括：本发明提供了一种适于甘薯种质资源盆栽的土壤基质及其筛选实验方法,属于农业种植技术领域。测定不同土壤基质下各种植株的各项生长指标,并利用隶属函数法、变异系数及主成分分析法对不同品种不同土壤基质处理条件下的生长指标进行综合评价,确定适宜的土壤基质配比,最适宜的土壤基质按百分比由如下几个成分组成：50％的壤土、20％的沙和30％的营养土。本发明对甘薯资源盆栽保存土壤基质进行了不同配比的科学筛选试验,通过对其反应生长情况的生长指标进行测定及分析,为甘薯资源盆栽保存提供了一个优化的基质配比和条件标准,该基质配比能更大程度上保证甘薯资源品种的活力,体现资源品种的种性差异特点。(The invention provides a soil matrix suitable for sweet potato germplasm resource pot culture and a screening experiment method thereof, belonging to the technical field of agricultural planting. Measuring various growth indexes of various plants under different soil matrixes, comprehensively evaluating the growth indexes under different soil matrix treatment conditions of different varieties by using a membership function method, a variation coefficient and a principal component analysis method, and determining a proper soil matrix ratio, wherein the most suitable soil matrix consists of the following components in percentage: 50% loam, 20% sand and 30% nutrient soil. The invention carries out scientific screening tests of different proportions on the sweet potato resource potted plant storage soil matrix, provides an optimized matrix proportion and condition standard for sweet potato resource potted plant storage by measuring and analyzing the growth indexes of the reaction growth conditions of the sweet potato resource potted plant storage soil matrix, and can ensure the vitality of sweet potato resource varieties to a greater extent by the matrix proportion and embody the characteristic of the property difference of the resource varieties.)

1. The soil matrix suitable for sweet potato germplasm resource pot culture is characterized by comprising the following components in percentage: 50% loam, 20% sand and 30% nutrient soil.

2. The soil matrix suitable for sweet potato germplasm resource pot culture as claimed in claim 1, wherein the components of the nutrient soil comprise moss peat, vermiculite, perlite, wetting agent and nutrient promoter.

3. The soil substrate suitable for sweet potato germplasm resource pot culture as claimed in claim 2, wherein the pH value of the nutrient soil medium is 5.8 ± 0.3.

4. A method for screening assays for soil substrates as defined in claim 1, comprising the steps of:

firstly, selecting seven soil matrixes with different proportions, namely

Substrate 1: 100% loam

Matrix 2: 75% loam + 25% sand

Matrix 3: 50% loam + 50% sand

Matrix 4: 50% of loam, 30% of sand and 20% of nutrient soil

Matrix 5: 50% of loam, 20% of sand and 30% of nutrient soil

Matrix 6: 50% of loam, 10% of sand and 40% of nutrient soil

Matrix 7: 100% of nutrient soil;

secondly, selecting six sweet potato resource varieties with different growth vigors as experimental objects;

thirdly, planting 3 plants in each pot of sweet potatoes of each resource variety in each soil matrix;

fourthly, measuring various growth indexes of various plants under different soil matrixes after the plants grow for 5 months, and comprehensively evaluating the growth indexes of different varieties under different soil matrix treatment conditions by utilizing a membership function method, a variation coefficient and a principal component analysis method to determine a proper soil matrix ratio.

5. The method of claim 4, wherein the growth indicators are petiole length, petiole diameter, stem diameter, internode length, diameter of stem base, number of branch bases, main vine length, photosynthetic rate, potato tuber yield, wet weight of ground parts, dry weight of ground parts.

6. The screening experiment method of soil matrix according to claim 4, wherein the number of the six sweet potato resource varieties is 1070, 130, A237, 1445, 1454 and A476.

Technical Field

The invention belongs to the technical field of agricultural planting, and particularly relates to a soil matrix suitable for sweet potato germplasm resource pot culture and an experimental method thereof.

Background

The germplasm resources are the core of crop breeding, and the collection and the preservation of the resources have very important significance for the crop breeding. The sweet potato germplasm resources are mainly stored in a national germplasm Guangzhou sweet potato nursery and a Xuzhou test tube nursery, the pot culture storage is an important mode for overwintering storage of the sweet potato resources in the national germplasm Guangzhou sweet potato nursery, and the selection of the pot culture soil has an important effect on the storage of the sweet potato germplasm resources.

The high-quality soil matrix proportion can keep the vitality of sweet potato resource varieties to the maximum extent and simultaneously reduce the investment of rich water and manpower. At present, soil for pot culture mainly adopts field culture soil or nutrient soil suitable for sweet potato culture, the scientific proportion of a system is lacked, the field soil is mainly loam or sandy loam, the phenomenon of hardening or weak fertility is often caused, the water loss rate of the nutrient soil is high, the soil is easy to cause drought, and the cost is relatively high. In order to optimize the substrate condition for sweet potato resource pot culture preservation, a soil substrate proportioning test suitable for sweet potato germplasm resource pot culture preservation is developed, and a condition standard is provided for sweet potato resource preservation.

Disclosure of Invention

Technical problem to be solved

The invention provides high-quality soil matrix proportion for sweet potato resource potting preservation mainly by screening the potting soil matrix, is convenient for sweet potato resource preservation, ensures the sexual activity of resource varieties and standardizes the condition foundation of potting preservation.

(II) technical scheme

Aiming at the defects in the prior art and achieving the purpose, the invention provides the following technical scheme: provides a soil matrix suitable for sweet potato germplasm resource pot culture, which comprises the following components in percentage: 50% loam, 20% sand and 30% nutrient soil.

Preferably, the nutrient soil comprises the following components: pure moss peat, vermiculite, perlite, a wetting agent and a nutrition promoter.

Preferably, the pH value of the nutrient soil medium is 5.8 +/-0.3.

The invention also provides a screening experiment method of the soil matrix, which comprises the following steps:

firstly, selecting seven soil matrixes with different proportions, namely

Substrate 1: 100% loam

Matrix 2: 75% loam + 25% sand

Matrix 3: 50% loam + 50% sand

Matrix 4: 50% of loam, 30% of sand and 20% of nutrient soil

Matrix 5: 50% of loam, 20% of sand and 30% of nutrient soil

Matrix 6: 50% of loam, 10% of sand and 40% of nutrient soil

Matrix 7: 100% of nutrient soil;

secondly, selecting six sweet potato resource varieties with different growth vigors as experimental objects, wherein the numbers of the six sweet potato resource varieties are 1070, 130, A237, 1445, 1454 and A476 respectively;

thirdly, planting 3 plants in each pot of sweet potatoes of each resource variety in each soil matrix;

fourthly, measuring various growth indexes of various plants under different soil matrixes after the plants grow for 5 months, and comprehensively evaluating the growth indexes of different varieties under different soil matrix treatment conditions by utilizing a membership function method, a variation coefficient and a principal component analysis method to determine a proper soil matrix ratio.

Preferably, the growth indexes are leaf stalk length, leaf stalk diameter, stem diameter, internode length, stem base diameter, base branch number, main vine length, photosynthetic rate, potato tuber yield, overground part wet weight and overground part dry weight.

The sweet potato resource number is the sweet potato resource number in the Guangzhou sweet potato garden of China germplasm.

(III) advantageous effects

Compared with the prior art, the invention has the following beneficial effects:

the invention carries out scientific screening tests of different proportions on the sweet potato resource potted plant storage soil matrix, provides an optimized matrix proportion and condition standard for sweet potato resource potted plant storage by measuring and analyzing the growth indexes of the reaction growth conditions of the sweet potato resource potted plant storage soil matrix, and can ensure the vitality of sweet potato resource varieties to a greater extent by the matrix proportion and embody the characteristic of the property difference of the resource varieties. The mixture ratio of the substrate is 50% loam, 20% sand and 30% nutrient soil, the material is conveniently obtained, and the cost is lower compared with that of pure nutrient soil.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The invention provides a soil matrix suitable for sweet potato germplasm resource potting, which comprises the following components in percentage: 50% loam, 20% sand and 30% nutrient soil.

The nutrient soil comprises the following components: pure moss peat, vermiculite, perlite, a wetting agent and a nutrition promoter.

The pH value of the nutrient soil medium is 5.8 +/-0.3.

Example 2, the invention provides a screening experiment method of soil matrix suitable for sweet potato germplasm resource pot culture,

1. the mixture ratio of the matrix is as follows:

according to the appropriate soil conditions for sweet potato field cultivation, the soil nutrition of the nutrient soil is combined, and the soil, sand and the nutrient soil for field cultivation are selected to be matched according to 7 different proportions:

the proportioning scheme is as follows:

substrate 1: 100% loam

Matrix 2: 75% loam + 25% sand

Matrix 3: 50% loam + 50% sand

Matrix 4: 50% of loam, 30% of sand and 20% of nutrient soil

Matrix 5: 50% of loam, 20% of sand and 30% of nutrient soil

Matrix 6: 50% of loam, 10% of sand and 40% of nutrient soil

Matrix 7: 100% nutrient soil

The nutrient profile of each soil matrix is shown in table 1:

TABLE 1 soil matrix nutrition situation table

2. Selecting resource varieties:

selecting 6 sweet potato variety resources with different growth vigors, respectively planting the healthy seedlings in different soil matrixes in 10-29 th of 2020, wherein each resource variety has 3 pots in each matrix, and 3 plants are planted in each pot.

3. Growth index investigation and analysis:

after the plants grow for 5 months, measuring growth indexes which can reflect the growth conditions of the sweet potato plants, such as the length of the leaf stalk, the diameter of the stem, the length of internodes, the diameter of the stem base, the number of branches at the base, the length of a main vine, the photosynthetic rate, the yield of the potato block, the wet weight of the overground part, the dry weight of the overground part and the like of each variety of plants under different substrate treatment conditions.

And comprehensively evaluating growth indexes of different varieties under different substrate treatment conditions by using a membership function method, a variation coefficient and a principal component analysis method, and determining the proportion of the soil substrate suitable for sweet potato germplasm resource pot preservation.

4. And (3) comprehensive evaluation results of growth indexes:

the growth indexes of different varieties under different substrate treatment conditions are comprehensively analyzed by a membership function method (table 2), and the sweet potato resource varieties 1070 and A237 are the most suitable soil substrates with the highest comprehensive evaluation value in the substrate 7, the sweet potato resource variety 130 is the most suitable soil substrates with the highest comprehensive evaluation value in the substrate 6, and the sweet potato resource varieties 1445, 1454 and A476 are the most suitable soil substrates with the highest comprehensive evaluation value in the substrate 5.

TABLE 2 comprehensive evaluation values of growth index membership functions of different resource varieties under different substrate conditions

The variation coefficient is adopted to evaluate the variation difference of the growth indexes of the same variety in different matrixes (table 3) and different varieties in the same matrix (table 4), and the membership function method is used to comprehensively evaluate the variation difference. The results show that the comprehensive membership function values of the index variation coefficients of the sweet potato resource varieties 1445, 1454 and A476 in matrix treatment are all higher than 1070, 130 and A237, which indicates that the growth of 1445, 1454 and A476 is influenced by the soil matrix more than 1070, 130 and A237, and the requirement on the soil matrix is higher (Table 3). The analysis of the variation coefficient of each index among varieties under different substrate treatment conditions shows (table 4), the substrate 5 has the highest comprehensive membership function value, which shows that the species difference among the varieties can be displayed better under the substrate 5 condition, the growth difference among the varieties is the largest, and the substrate 5 is the most suitable growth soil substrate of 1070, 130 and A237, so that the substrate 5 can be used for better ensuring the preservation of the species characteristics of each resource variety, and is suitable for the pot culture preservation of the sweet potato germplasm resources.

TABLE 3 evaluation of index variation coefficient and comprehensive membership function between substrate treatments under different sweet potato resource varieties

TABLE 4 evaluation of the coefficient of variation and the comprehensive membership function of each index between varieties under different substrate treatment conditions

Different growth indexes of sweet potato plants have certain correlation and do not exist independently, so that certain information overlapping exists, and in order to carry out more reasonable analysis on the growth indexes of different resource varieties under different substrate conditions, a principal component analysis method is adopted to carry out further analysis on the growth indexes. To eliminate the differences between the varieties, we converted each growth index into a growth index coefficient for analysis, the growth index coefficient being the index value of different substrate treatments/substrate 1 index value, the individual growth index coefficient being the average of the individual growth index coefficients of different varieties (table 5).

TABLE 5 average individual index coefficient of each variety under different substrate treatment conditions

The principal component analysis result shows that certain correlation exists between every two indexes in the correlation analysis (table 6), which indicates that information is overlapped between the indexes, the principal component extraction is carried out on 11 growth indexes (table 7), the 11 growth indexes are converted into 3 independent principal components, the cumulative contribution rate of the 3 principal components reaches 84.012%, the vast majority of information of the 11 growth indexes can be reflected, the principal component comprehensive evaluation is carried out on the factor values of the three principal components (table 8), and the result indicates that the substrate 5 has the highest comprehensive evaluation value and is most suitable for pot culture storage of sweet potato resources.

TABLE 6 correlation analysis of growth indicators

TABLE 7 principal Components extraction analysis of growth indicators

TABLE 8 comprehensive evaluation of principal Components under different substrate conditions

In conclusion, screening of 7 kinds of potted matrixes by different sweet potato resource varieties shows that the matrix 5 is most suitable for potted preservation of sweet potato germplasm resources, and can ensure the vitality of the resource varieties to a greater extent and reflect the sexual characteristics of the different resource varieties under the condition of the matrix 5.