Plant bionic skin lipid film and preparation method thereof
阅读说明：本技术 一种植物仿生皮脂膜及其制备方法 (Plant bionic skin lipid film and preparation method thereof ) 是由 章万领 于 2021-09-23 设计创作，主要内容包括：本发明提供一种植物仿生皮脂膜及其制备方法,涉及护肤品技术领域。该植物仿生皮脂膜包括：神经酰胺,胆固醇,棕榈酸,硬脂酸。本发明中植物仿生皮脂膜将天然脂质成分经过黄金配比,模仿人体皮脂结构,形成植物仿生皮脂膜,从而减少肌肤水分流失,缓解干燥和炎症,构建并强化肌肤锁水、防护屏障。本发明的制备方法可以将各组分充分混合,让得到的皮脂膜均一性好,使得皮脂膜对肌肤的防护效果更好。(The invention provides a plant bionic skin fat membrane and a preparation method thereof, and relates to the technical field of skin care products. The plant bionic skin lipid membrane comprises: ceramide, cholesterol, palmitic acid, stearic acid. According to the plant bionic sebum membrane, natural lipid components are matched with gold to simulate the sebum structure of a human body, so that the plant bionic sebum membrane is formed, the moisture loss of the skin is reduced, dryness and inflammation are relieved, and the skin water locking and protection barriers are constructed and strengthened. The preparation method can fully mix the components, so that the obtained sebum membrane has good uniformity, and the sebum membrane has better protective effect on skin.)
1. A plant bionic skin lipid membrane is characterized by comprising the following components in parts by weight: 35-45 parts of ceramide, 20-30 parts of cholesterol, 10-18 parts of palmitic acid and 5-12 parts of stearic acid.
2. The plant bionic skin lipid film as claimed in claim 1, characterized by further comprising 1-10 parts by weight of water-supplementing and moisture-preserving components, wherein the water-supplementing and moisture-preserving components comprise tea extract and camellia seed oil.
3. The plant bionic skin lipid membrane as claimed in claim 1, which is characterized by further comprising 0.5-3 parts of sodium polyacrylate grafted starch, 0.1-2 parts of methacrylate cross-linked polymer, 3-8 parts of cetyl ethyl hexanoate, 1-10 parts of cholesterol sulfate, 0.5-3.5 parts of maltodextrin and 2-7 parts of ethylhexyl glycerol by weight.
4. The plant biomimetic sebum membrane according to claim 3, wherein the cholesterol sulfate is an oxidized cholesterol sulfate.
5. A method for preparing a plant biomimetic sebum membrane according to any one of claims 1-4, comprising the steps of:
mixing palmitic acid and stearic acid, heating, and keeping the temperature at 80-85 ℃ for 5-10min to obtain a first mixture; mixing ceramide and cholesterol, adding deionized water, stirring at 80-90 deg.C for 30-40min, maintaining at 85-90 deg.C for 10-15min to obtain a second mixture, mixing the two mixtures to obtain a mixed solution, cooling to 45-55 deg.C, and cooling.
6. The method according to claim 5, wherein the heating is carried out at a heating rate of 1 to 4 ℃/min.
7. The method of claim 5, wherein the relative density of the second mixture is 1.13 to 1.35.
8. The preparation method of claim 5, further comprising stirring the mixed solution at 30-50r/min for 10-18min before cooling.
9. The method of claim 8, further comprising homogenizing under high pressure at 70-80MPa for 5-15min after cooling.
10. The method of claim 9, further comprising sterilizing for 5-12min after homogenizing.
Technical Field
The invention relates to the technical field of skin care products, in particular to a plant bionic skin lipid membrane and a preparation method thereof.
Background
The sebum membrane is the outermost layer of the skin and serves as a natural moisturizing barrier, and can lock moisture in the skin, introduce moisturizing ingredients into the skin to be absorbed, and prevent external substances from invading. The sebum membrane can keep the water content of skin in a normal state, prevent excessive evaporation of skin moisture, namely lock water, and enable the skin to be soft, smooth and glossy. However, in a severe environment or in improper care, the sebum membrane is easily broken, and the skin protected by the sebum membrane is lost, so that the skin becomes rough, dry and sensitive. The existing sebum membrane has poor bionic effect and poor protective effect on skin.
Disclosure of Invention
The invention aims to provide a plant bionic sebum membrane, which is formed by matching natural lipid components with gold and simulating a human body sebum structure, so that the moisture loss of skin is reduced, dryness and inflammation are relieved, and a water-locking and protective barrier of the skin is constructed and strengthened.
The invention also aims to provide a preparation method of the plant bionic sebum membrane, which can fully mix all the components, so that the obtained sebum membrane has good uniformity and better skin protection effect.
The technical problem to be solved by the invention is realized by adopting the following technical scheme.
The invention provides a plant bionic skin lipid membrane which comprises the following components in parts by weight: 35-45 parts of ceramide, 20-30 parts of cholesterol, 10-18 parts of palmitic acid and 5-12 parts of stearic acid.
The invention provides a preparation method of a plant bionic skin lipid membrane, which comprises the following steps:
mixing palmitic acid and stearic acid, heating to 70-80 deg.C, and keeping the temperature at 80-85 deg.C for 5-10min to obtain a first mixture; mixing ceramide and cholesterol, adding deionized water, stirring at 80-90 deg.C for 30-40min, maintaining at 85-90 deg.C for 10-15min to obtain a second mixture, mixing the two mixtures to obtain a mixed solution, cooling to 45-55 deg.C, and cooling.
The plant bionic skin lipid membrane and the preparation method thereof provided by the embodiment of the invention have the following beneficial effects:
according to the invention, the skin-like structure is reproduced by ceramide, cholesterol and fatty acid components, and the skin lipid layer is enhanced, so that the moisture evaporation is inhibited, the inflammatory reaction is relieved, and the skin barrier is enhanced and toughened for sensitive and tender skin. And through reasonably proportioning the components, the synergistic promotion effect of the components is better, the components are reasonably matched, and the water replenishing and locking effect is obviously enhanced. According to the preparation method disclosed by the invention, all the components are fully mixed, so that the obtained sebum membrane has good uniformity, and the skin protection effect of the sebum membrane is better.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present invention will be described in detail below with reference to specific examples.
A plant bionic skin lipid membrane comprises the following components in parts by weight: 35-45 parts of ceramide, 20-30 parts of cholesterol, 10-18 parts of palmitic acid and 5-12 parts of stearic acid.
Ceramide is a kind of phospholipid with ceramide as a skeleton, mainly comprising ceramide phosphorylcholine and ceramide phosphorylethanolamine, is a main component of cell membranes, 40% -50% of sebum in the horny layer is composed of ceramide, and the ceramide is a main part of intercellular matrix and plays an important role in keeping the moisture balance of the horny layer. Ceramides have a strong ability to associate with water molecules, and they maintain skin moisture by forming a network in the stratum corneum. Therefore, the ceramide has the effects of keeping skin moisture, obviously enhancing the adhesive force between keratinocytes, improving the dryness of the skin, reducing skin desquamation, increasing the thickness of epidermal horny layer, improving the water holding capacity of the skin, reducing wrinkles, enhancing the elasticity of the skin, delaying skin aging, moisturizing and repairing, and strengthening the muscle bottom.
The cholesterol is a derivative of cyclopentane multi-hydrogen phenanthrene, and has a chemical formula of C27H46OWhite or yellowish crystals, which are the main steroids in mammals, play an important role in basic cell life activities. Cholesterol is an important constituent component forming cell membranes, the cell membranes surround each cell of a human body, the cholesterol is a basic constituent component of the cholesterol, accounts for more than 20% of plasma membrane lipid, mainly acts as an emulsifier, a emollient and viscosity control, has a risk coefficient of 1, is safe, can be used at ease, generally has no influence on pregnant women, and has no pox-causing property of cholesterol. The human skin secretion contains a certain amount of cholesterol and its derivatives, and has effects of smoothing and keeping moisture. Cholesterol has no irritation to skin and no photosensitization, and the skin-moistening type cosmetic contains 1.4% of cholesterol, has the functions of enhancing other active agents, and also has the effects of smoothing, moisturizing and stabilizing skin.
Palmitic acid, also known as palmitic acid, has the chemical formula C16H32O2The yellow pearlescent flake is a saturated higher fatty acid, is white and pearlescent, is insoluble in water, slightly soluble in petroleum ether, soluble in ethanol and soluble in ether, chloroform and acetic acid, is widely present in nature, and almost all grease contains palmitic acid components in different quantities. Stearic acid of formula C18H36O2Molecular weight 284.48, is a compound, octadecanoic acid, used in cosmetics for emulsification. The palmitic acid and the stearic acid are used in a matching way, so that the lipid structure can be consolidated, and the skin barrier can be strengthened.
In this example, ceramide, cholesterol, and fatty acid are all components of the stratum corneum, and ceramide constitutes the stratum corneum cutin of the stratum corneum, 40% of intercellular lipid, and is an essential component for the formation and function of the stratum corneum structure. Cholesterol is an ingredient that constitutes about 30% of the normal skin barrier and has a synergistic effect in enhancing moisture and promoting product absorption. Fatty acids constitute about 25% of the skin lipids and help to increase product absorption and retention of moisture. A human body lipid-like structure system is reproduced through natural lipid components, a similar skin structure is reproduced by applying ceramide, cholesterol and fatty acid components, and the skin lipid layer is enhanced, the water evaporation is inhibited, the inflammatory reaction is relieved, and the skin barrier is enhanced and toughened for sensitive and tender skin. The fatty acid can be stearic acid and palmitic acid, the stearic acid and the palmitic acid are not only components of natural oil, the stearic acid and the palmitic acid can be used for consolidating lipid structures, strengthening skin barriers and further enhancing the protection of a skin lipid membrane on skin, and in addition, the stearic acid also has an emulsifying effect, so that the emulsifying effect of the product can be improved, the product can be absorbed by the skin more easily, and the skin water shortage condition is obviously improved. In addition, the components are reasonably proportioned, so that the synergistic promotion effect of the components is better, the components are reasonably matched, and the sebum structure of a human body is better reproduced, so that the moisture loss of the skin is reduced, the dryness and the inflammation are relieved, and the skin water locking and the protective barrier are constructed and strengthened.
In the embodiment, the tea-leaf moisturizing and skin-protecting tea further comprises 1-10 parts of a moisturizing component by weight, wherein the moisturizing component comprises a tea extract and camellia seed oil.
Tea extract is an active component extracted from green tea leaves, and mainly comprises tea polyphenol (catechin), caffeine, aromatic oil, water, minerals, pigments, carbohydrates, proteins, amino acids, vitamins, etc. The tea polyphenols have effects of resisting oxidation, scavenging free radicals, reducing serum total cholesterol, triglyceride and low density lipoprotein cholesterol of hyperlipidemia, and recovering and protecting vascular endothelial function. Tea polyphenols can block lipid peroxidation process and improve activity of enzyme in human body. Tea polyphenols can kill botulinum and spores, inhibit the activity of bacterial exotoxin, and have antibacterial effect on various pathogenic bacteria causing diarrhea, respiratory tract and skin infection. Tea polyphenols stimulate the change of antibody activity by increasing and maintaining the total amount of human immunoglobulin at a high level, thereby improving the overall immunity of human and promoting the self-conditioning function of human body.
The camellia seed oil has the effects of sterilizing and diminishing inflammation, astringing pores, treating common cold, cough, rhinitis and asthma, improving dysmenorrheal, irregular menstruation, genital infection and the like, can make skin fresh and smooth, has smoother skin, has the antibacterial and anti-inflammatory effects of the tea tree essential oil, and can quickly permeate into hair follicles for conditioning. The tea tree essential oil is suitable for oily and acne skin, and can be used for treating suppurative wound, burn, sunburn, tinea pedis and dandruff, refreshing brain, recovering activity, and resisting depression.
Tea polyphenol in the tea extract has the effects of resisting oxidation and removing free radicals, can block the lipid peroxidation process, improves the activity of enzymes in a human body, has an obvious antibacterial effect, and has an obvious relieving effect on dermatitis. The camellia seed oil has the effects of sterilizing and diminishing inflammation, astringing pores, preserving moisture and lubricating, and particularly can condition skin hair follicles. After the two are matched for use, the skin surface layer and the skin lining are improved, so that the protective effect of the sebum membrane on the skin is further improved, the skin is more conveniently replenished with water after the hair follicle is cleared, and the moisturizing effect of the sebum membrane is obviously enhanced.
In the embodiment, the coating further comprises 0.5-3 parts of sodium polyacrylate grafted starch, 0.1-2 parts of methacrylate cross-linked polymer, 3-8 parts of cetyl ethyl hexanoate, 1-10 parts of cholesterol sulfate, 0.5-3.5 parts of maltodextrin and 2-7 parts of ethylhexyl glycerol by weight.
The graft copolymer of sodium polyacrylate grafted starch and starch is a kind of new-type high-molecular material, and is characterized by that it uses hydrophilic and semi-rigid starch macromolecule as skeleton, utilizes physicochemical method to initiate production of free radical, and makes it produce copolymerization reaction with vinyl monomer, and utilizes the introduction of different functional groups and regulation of proportion of hydrophilic and oleophilic chain segment structures to make it possess polysaccharide compound, intermolecular action force and reactivity, and possess the stability of mechanical and biological action for synthesizing high molecule and development capacity of linear method structure. The addition of the graft copolymer to the incompatible low molecular weight solvent mixture enables the two phases to be emulsified to form a stable emulsion, and the incompatible high molecular weight and low molecular weight liquid phases can also be mixed by virtue of the emulsification of the graft copolymer. The graft modified starch has certain emulsification function due to the existence of the starch main chain and the polymer branch chain, can improve the miscibility of slurry, is mainly used as a binding agent, a film forming agent and a thickening agent, and has no pox-causing property.
The methacrylate cross-linked polymer mainly functions as a film forming agent and a thickening agent and has no pox-causing property. Cetyl ethylhexanoate is a cheap substitute product of natural squalane, is an emollient with particularly excellent waterproof performance, and has the advantages of good air permeability and spreadability, no stickiness, thickening effect and capability of moistening and softening skin. The cholesterol sulfate, in particular to the oxidized cholesterol sulfate, has good anti-inflammatory effect and good relieving effect on allergic dermatitis.
Maltodextrin, wherein the higher the hydrolysis degree of maltodextrin, the larger the DE value is, and the larger the oxygen solubility, sweetness, permeability, fermentability, browning reaction and freezing point depression of the product are; the poorer the structure, viscosity, pigment stability and crystallization resistance, the lower the sweetness, no peculiar smell, easy digestion, low heat, good solubility, small fermentability, good filling effect, difficult moisture absorption, strong thickening property, good carrier property, good stability and difficult deterioration. Maltose may also be a humectant and skin conditioner.
Ethylhexyl glycerin is an important representative variety of 'no-additive' preservatives, is a widely used preservative synergist, has a moisturizing effect, and can impart a pleasant skin feel to a formula. It can greatly improve the broad spectrum of a plurality of traditional preservatives (such as phenoxyethanol), and the ethylhexyl glycerin can reduce the activity of bacteria by reducing the surface tension of the cell walls of the microorganisms, so that the preservative system is more effective and faster.
When the sodium polyacrylate grafted starch, the methacrylate cross-linked polymer, the cetyl ethyl hexanoate and the maltodextrin are selected to be matched for use, the thickening effect is better, the film forming of the sebum film is facilitated, the sebum film can also have good waterproof performance, and the water locking performance of the sebum film is better. In addition, the cetyl ethyl hexanoate has good air permeability and spreadability, and when the sebum membrane is used, the comfort level of the use can be improved, so that the used skin does not have sticky feeling and suffocating feeling. And secondly, the preservative function of the leather fat film can be increased by matching with the ethylhexyl glycerin, and the preservative film also has a moisturizing effect, so that the pleasure feeling and the comfort degree of the leather fat film are further improved.
A preparation method of a plant bionic skin lipid membrane comprises the following steps:
mixing palmitic acid and stearic acid, heating at a heating rate of 1-4 ℃/min, and keeping the temperature at 80-85 ℃ for 5-10min to obtain a first mixture; mixing ceramide, cholesterol and cholesterol sulfate, adding 500mL of 200-mL deionized water, stirring at 80-90 ℃ for 30-40min, preserving heat at 85-90 ℃ for 10-15min to obtain a second mixture, wherein the relative density of the second mixture is 1.13-1.35, combining the two mixtures to obtain a mixed solution, stirring at 30-50r/min for 10-18min, then cooling to 45-55 ℃, homogenizing under 70-80MPa for 5-15min, then sterilizing for 5-12min, and cooling.
In the embodiment, the palmitic acid and the stearic acid are heated and melted, so that the uniformity of the sebum membrane can be improved when the palmitic acid and the stearic acid are subsequently melted with other components, and the effect is better after the palmitic acid and the stearic acid are used. The heating rate is controlled to be 1-4 ℃/min, so that the phenomenon that the melting speed is too slow and the melting speed is changed due to overheating can be avoided, and meanwhile, the uniform heating is beneficial to the good melting degree and the good melting effect of the two. Heating, and keeping at 80-85 deg.C for 5-10min to further assist in mixing the two components and improve mixing effect. Adding ceramide, cholesterol and cholesterol into deionized water for heating, so that the dissolving speed of the ceramide, the cholesterol and the cholesterol can be accelerated, selecting the deionized water to avoid the cholesterol sulfate from reacting with ions to generate salt compounds, and influencing the using effect of a sebum membrane, and selecting stirring at 80-90 ℃ for 30-40min for better dissolving effect of the ceramide, the cholesterol and the cholesterol, so that the ceramide, the cholesterol and the cholesterol can be completely dissolved, and the mixing effect is best. The mixture can be kept at 85-90 ℃ for 10-15min to ensure that the mixture is stable and the solidification after cooling is avoided. The relative density of the second mixture is set, so that the phenomenon that the density of the obtained product is too thin or too thick due to too much or too little deionized water can be avoided, and the using effect is poor. Mixing the two mixtures, stirring at 30-50r/min for 10-18min to obtain a better mixing effect, and cooling and homogenizing at 70-80MPa for 5-15min to improve the uniformity of the product and the mixing effect of the components, thereby improving the use effect of the product. The sterilization can improve the cleanliness of the product, thereby improving the safety in use.
In the embodiment, partial components are separately treated, so that the mutual synergistic promotion effect among the components is stronger, the water replenishing and locking effect of the sebum membrane is further obviously improved, and the skin barrier is enhanced.
The features and properties of the present invention are described in further detail below with reference to examples.
Example 1
A plant bionic skin lipid membrane comprises the following components:
35g of ceramide, 20g of cholesterol, 10g of palmitic acid and 5g of stearic acid.
A preparation method of a plant bionic skin lipid membrane comprises the following steps:
mixing palmitic acid and stearic acid, heating at a heating rate of 1 ℃/min, and keeping the temperature at 80 ℃ for 5min to obtain a first mixture; mixing ceramide and cholesterol, adding 200mL of deionized water, stirring at 80 ℃ for 30min, keeping the temperature at 85 ℃ for 10min to obtain a second mixture, wherein the relative density of the second mixture is 1.13, combining the two mixtures to obtain a mixed solution, stirring at 30r/min for 10min, then cooling to 45 ℃, homogenizing under 70MPa for 5min, then sterilizing for 5min, and cooling.
Example 2
A plant bionic skin lipid membrane comprises the following components:
45g of ceramide, 30g of cholesterol, 18g of palmitic acid and 12g of stearic acid.
A preparation method of a plant bionic skin lipid membrane comprises the following steps:
mixing palmitic acid and stearic acid, heating at a heating rate of 4 ℃/min, and keeping the temperature at 85 ℃ for 10min to obtain a first mixture; mixing ceramide and cholesterol, adding 500mL of deionized water, stirring at 90 ℃ for 40min, keeping the temperature at 90 ℃ for 15min to obtain a second mixture, wherein the relative density of the second mixture is 1.35, mixing the two mixtures to obtain a mixed solution, stirring at 50r/min for 18min, then cooling to 55 ℃, homogenizing under 80MPa for 15min, then sterilizing for 12min, and cooling.
Example 3
A plant bionic skin lipid membrane comprises the following components:
40g of ceramide, 25g of cholesterol, 15g of palmitic acid and 5g of stearic acid.
A preparation method of a plant bionic skin lipid membrane comprises the following steps:
mixing palmitic acid and stearic acid, heating at a heating rate of 2 ℃/min, and keeping the temperature at 82 ℃ for 8min to obtain a first mixture; mixing ceramide and cholesterol, adding 300mL of deionized water, stirring at 85 ℃ for 35min, keeping the temperature at 88 ℃ for 12min to obtain a second mixture, wherein the relative density of the second mixture is 1.23, combining the two mixtures to obtain a mixed solution, stirring at 40r/min for 15min, then cooling to 50 ℃, homogenizing at 75MPa for 10min, then sterilizing for 8min, and cooling.
Example 4
A plant bionic skin lipid membrane comprises the following components:
38g of ceramide, 22g of cholesterol, 24g of palmitic acid, 10g of stearic acid and 5g of water-replenishing and moisture-preserving component, wherein the water-replenishing and moisture-preserving component comprises tea extract and camellia seed oil.
A preparation method of a plant bionic skin lipid membrane comprises the following steps:
mixing palmitic acid and stearic acid, heating at a heating rate of 2.5 ℃/min, and keeping the temperature at 81 ℃ for 7min to obtain a first mixture; mixing ceramide and cholesterol, adding 280mL of deionized water, stirring at 86 ℃ for 33min, keeping the temperature at 87 ℃ for 12min to obtain a second mixture, wherein the relative density of the second mixture is 1.18, combining the two mixtures to obtain a mixed solution, adding a water supplementing and moisturizing component, stirring at 36r/min for 16min, then cooling to 52 ℃, homogenizing at 78MPa for 9min, then sterilizing for 11min, and cooling.
Example 5
A plant bionic skin lipid membrane comprises the following components:
42g of ceramide, 28g of cholesterol, 13g of palmitic acid, 7g of stearic acid, 8g of cholesterol sulfate, 1g of sodium polyacrylate grafted starch, 1.5g of methacrylate cross-linked polymer, 4g of cetyl ethylhexanoate, 2g of maltodextrin and 4g of ethylhexyl glycerol.
A preparation method of a plant bionic skin lipid membrane comprises the following steps:
mixing palmitic acid and stearic acid, heating at a heating rate of 1.5 ℃/min, and keeping the temperature at 82 ℃ for 6min to obtain a first mixture; mixing ceramide, cholesterol and cholesterol sulfate, adding 400mL deionized water, stirring at 87 ℃ for 32min, keeping the temperature at 86 ℃ for 11min to obtain a second mixture, wherein the relative density of the second mixture is 1.32, combining the two mixtures to obtain a mixed solution, stirring at 48r/min for 14min, then cooling to 48 ℃, adding sodium polyacrylate grafted starch, methacrylate cross-linked polymer, cetyl ethyl hexanoate, maltodextrin and ethylhexyl glycerol, homogenizing at 74MPa for 8.5min, then sterilizing for 6.5min, and cooling.
Example 6
A plant bionic skin lipid membrane comprises the following components:
38g of ceramide, 26g of cholesterol, 14g of palmitic acid, 8g of stearic acid, 2g of deoxycholesterol sulfate, 3g of a moisturizing component, wherein the moisturizing component comprises a tea extract and camellia oil, 1.5g of sodium polyacrylate grafted starch, 0.8g of methacrylate cross-linked polymer, 3.5g of cetyl ethylhexanoate, 2.5g of maltodextrin and 3g of ethylhexyl glycerol.
A preparation method of a plant bionic skin lipid membrane comprises the following steps:
mixing palmitic acid and stearic acid, heating at a heating rate of 1 ℃/min, and keeping the temperature at 82 ℃ for 7min to obtain a first mixture; mixing ceramide, cholesterol and deoxycholesterol sulfate, adding 380mL deionized water, stirring at 83 ℃ for 32min, keeping the temperature at 88 ℃ for 12min to obtain a second mixture, wherein the relative density of the second mixture is 1.28, combining the two mixtures to obtain a mixed solution, adding water supplementing and moisturizing components, stirring at 33r/min for 12min, then cooling to 47 ℃, adding sodium polyacrylate grafted starch, methacrylate cross-linked polymer, cetyl ethyl hexanoate, maltodextrin and ethylhexylglycerin, homogenizing at 72MPa for 8min, then sterilizing for 7.5min, and cooling.
Example 7
A plant bionic skin lipid membrane comprises the following components:
41g of ceramide, 21g of cholesterol, 11g of palmitic acid and 6g of stearic acid.
A preparation method of a plant bionic skin lipid membrane comprises the following steps:
mixing palmitic acid and stearic acid, heating, and keeping the temperature at 83 ℃ for 8min to obtain a first mixture; mixing ceramide and cholesterol, adding deionized water, stirring at 88 deg.C for 36min, maintaining at 88 deg.C for 12min to obtain a second mixture, mixing the two mixtures to obtain a mixed solution, cooling to 48 deg.C, and cooling.
1. Safety test
The skin lipid film prepared in the embodiment of the invention is subjected to a human body safety test, the test method refers to a skin repeatability open smearing test in 2015 cosmetic safety technical Specification, and the test method comprises the following steps: 70 persons aged 18-60 years are selected and randomly divided into 7 groups, and the 7 groups correspond to the sebum membrane obtained by the embodiments 1-7 of the invention, and the specific use method is as follows: selecting forearm skin area of 40mm21g of the skin reaction liquid is applied once, 2 times of daily application and 7 days of continuous application, the skin reaction condition of a subject is recorded, and the skin reaction result of the subject is judged according to the highest reaction course of all observations, and the result is as follows:
TABLE 1 Scoring standards
TABLE 2 human body safety test results
Examples/days
The next day
The fourth day
Day six
Total result
1
0
0
0
Are all negative
2
0
0
0
Are all negative
3
0
0
0
Are all negative
4
0
0
0
Are all negative
5
0
0
0
Are all negative
6
0
0
0
Are all negative
7
0
0
0
Are all negative
As can be seen from Table 2, the sebum films obtained in the examples of the present invention passed the safety test on human skin.
2. Test of moisturizing Effect
This test tests the sebum membranes of examples 1-7 of the invention.
The test subjects selected were 70 women over 30-40 years old or women who required moisturizing, and were randomly divided into 7 groups of 10 groups, each group had no significant difference in age and skin properties, and were comparable, and the sebum films of examples 1-7 were used for 7 groups. The using method and the testing method comprise the following steps: the sebum membrane is evenly smeared on the arm cleaned by clear water of a subject, the skin moisture content before smearing, after smearing for 2h, 4h and 8h is respectively tested by a skin moisture tester, each group of results is an average value, and the test results are as follows:
table 3 moisturizing effect test results
As can be seen from table 3, the moisturizing effect of example 6 is better than that of other examples, and the moisturizing and long-acting water-locking effects are very good, and in comparison, the long-acting water-locking function of example 7 is worse, which indicates that the synergistic promotion effect of the components of the skin lipid film composition is better in the adding step of the auxiliary materials, and the components are reasonably matched with each other, so that the significant moisturizing and water-locking effects are obtained.
In summary, according to the plant bionic sebum membrane and the preparation method of the plant bionic sebum membrane of the embodiment of the invention, the natural lipid components are proportioned by gold to simulate the sebum structure of a human body, the similar skin structure is reproduced through the ceramide, the cholesterol and the fatty acid components to form the plant bionic sebum membrane, the skin lipid layer is enhanced, the water evaporation is inhibited, the inflammatory reaction is relieved, and the skin barrier is enhanced and toughened for sensitive and tender skin, so that the water loss of the skin is reduced, the dryness and the inflammation are relieved, and the water-locking and protection barriers of the skin are constructed and enhanced. The preparation method can fully mix the components, so that the obtained sebum membrane has good uniformity, and the sebum membrane has better protective effect on skin.
The embodiments described above are some, but not all embodiments of the invention. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.