阅读说明：本技术 一种具有抑菌活性的中药组合物及应用 (Traditional Chinese medicine composition with antibacterial activity and application ) 是由 刘东玲 刘小凤 侯雯倩 宁艳梅 海洋 郭清毅 苏敬 董劲曲 徐兰 于 2021-09-10 设计创作，主要内容包括：本发明属于中药领域,具体涉及一种具有抑菌活性的中药组合物及应用。所述具有抑菌活性的中药组合物包括黄芩、金银花、板蓝根、连翘、山楂、薄荷、五倍子；所述中药组合物对金黄色葡萄球菌、大肠杆菌、白色念珠菌均有显著的抑菌效果,且其抑菌效果优于单味中药；在降低药物使用剂量的同时,降低毒性和细菌耐药性；所述中药组合物稳定性高,同时操作简单、生产成本低、提取率高、生产应用方便；提供的中药组合物可用于制备消杀产品和健康用品,包括具有抑菌功能的洗手液、喷雾剂、抑菌香皂等,不但拓展了中药开发应用的范围与深度,同时将中药抑菌成分应用于产品中,该技术的市场应用前景广阔必会受到广大消费者青睐。(The invention belongs to the field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition with antibacterial activity and application thereof. The Chinese medicinal composition with antibacterial activity comprises Scutellariae radix, flos Lonicerae, radix Isatidis, fructus forsythiae, fructus crataegi, herba Menthae, and Galla chinensis; the traditional Chinese medicine composition has obvious bacteriostatic effect on staphylococcus aureus, escherichia coli and candida albicans, and the bacteriostatic effect is superior to that of a single traditional Chinese medicine; the toxicity and the bacterial drug resistance are reduced while the dosage of the drug is reduced; the traditional Chinese medicine composition has high stability, and is simple to operate, low in production cost, high in extraction rate and convenient to produce and apply; the provided traditional Chinese medicine composition can be used for preparing disinfection products and health products, including hand sanitizer, spray, bacteriostatic soap and the like with bacteriostatic functions, so that the range and the depth of development and application of traditional Chinese medicines are expanded, meanwhile, bacteriostatic components of the traditional Chinese medicines are applied to the products, and the technology has wide market application prospect and is certainly favored by consumers.)

1. The traditional Chinese medicine composition with bacteriostatic activity is characterized by comprising the following components in parts by mass: 1-2 parts of scutellaria baicalensis, 1-2 parts of honeysuckle, 1-2 parts of isatis root, 1-2 parts of fructus forsythiae, 1-2 parts of hawthorn, 1-2 parts of mint and 1-2 parts of gallnut.

2. The traditional Chinese medicine composition of claim 1, wherein the traditional Chinese medicine composition comprises the following components in parts by mass: 2 parts of scutellaria baicalensis, 1 part of honeysuckle, 2 parts of isatis root, 2 parts of fructus forsythiae, 1 part of hawthorn, 1 part of mint and 2 parts of gallnut.

3. The traditional Chinese medicine composition of claim 1, further comprising the following components in parts by mass: 1-2 parts of schisandra chinensis, 1-2 parts of cinnamon and 1-2 parts of white paeony root.

4. The traditional Chinese medicine composition of claim 3, which comprises the following components in parts by mass: : 2 parts of scutellaria baicalensis, 1 part of honeysuckle, 2 parts of isatis root, 2 parts of fructus forsythiae, 1 part of hawthorn, 1 part of mint, 2 parts of gallnut, 1 part of schisandra chinensis, 1 part of cinnamon and 1 part of radix paeoniae alba.

5. The method for preparing a Chinese medicinal composition according to any one of claims 1 to 4, wherein the method comprises the following steps: boiling the Chinese medicinal composition in water; filtering; heating and concentrating the filtrate to obtain the product.

6. The method of claim 5, comprising the steps of:

(1) taking the Chinese medicinal composition, adding ten times of distilled water, heating with strong fire to boil, and heating with slow fire for 1 hr;

(2) filtering, taking the dregs of a decoction, adding ten times of distilled water, and repeating the step (1);

(3) filtering, mixing filtrates, heating and concentrating to 1g/mL, standing at room temperature for 24h, centrifuging to obtain supernatant, and autoclaving.

7. The use of a Chinese medicinal composition as claimed in any one of claims 1 to 4 in the preparation of an antibacterial medicament.

8. The Chinese medicinal composition of any one of claims 1-4, wherein the Chinese medicinal composition is added with a pharmaceutically acceptable carrier to prepare any one pharmaceutically acceptable dosage form.

9. The Chinese medicinal composition of claim 8, wherein the dosage form comprises tablets, injections, oral liquids, and sprays.

10. The use of the Chinese medicinal composition of any one of claims 1-4 in the preparation of hand sanitizer and bacteriostatic soap.

Technical Field

The invention belongs to the field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition with antibacterial activity and application thereof.

Background

The traditional Chinese medicine bacteriostatic agent can prevent and treat diseases, disinfect and clean air, has few adverse reactions and does not need to add any chemical reagent. So far, a plurality of scholars show that a plurality of traditional Chinese herbal medicines have obvious antibacterial and antiviral effects through modern pharmacological research, the traditional Chinese medicine bacteriostatic agent makes new progress in the field of disinfection, and the application of the traditional Chinese medicine in the bacteriostatic agent not only opens up the traditional Chinese medicine source, but also expands the application range of the traditional Chinese medicine. The traditional Chinese medicine bacteriostatic agent is natural in material, safe, nontoxic and aromatic in smell. Some traditional Chinese medicine components have multiple target points and obvious in-vitro antiviral and antibacterial effects, can effectively inhibit the replication of the disease source and block the spread of the disease source, have wide application value, and are not easy to generate drug resistance due to the synergistic effect of multiple components in the formula. The traditional Chinese medicine disinfectant has the characteristics of low toxicity, mild bacteriostasis and wide bacteriostasis spectrum, and can be well popularized.

Pathogenic microorganism refers to microorganism which can invade human body and cause infection and even infectious disease, or pathogen, and belongs to the pathogenic factors of insect pathogen in exogenous pathogenic factors in traditional Chinese medicine. The parasites have the property of fleeing away and are related to wind pathogen, and ancient people mostly think that wind, damp-heat, dirty and other environments are easy to generate parasites, which are the important causes of the parasites, as in Yi Zong jin Jian, Xuan: "this syndrome always affects the skin by wind-heat and dampness, and is transformed into worms due to long-term wind, also known as Keqin: the Chinese medicine uses pungent and pungent substances for dispelling wind, bitter and dry for eliminating dampness, heat-clearing and detoxicating, sour and astringent substances for removing pathogenic microorganisms.

The invention provides a traditional Chinese medicine composition with a remarkable antibacterial effect, which comprises scutellaria baicalensis, honeysuckle, isatis root, fructus forsythiae, hawthorn, mint and gallnut; the traditional Chinese medicine composition has better antibacterial effect than a single traditional Chinese medicine; is helpful for reducing the dosage of the medicine, toxicity and bacterial drug resistance.

Disclosure of Invention

Aiming at the technical problems, the invention provides a traditional Chinese medicine composition with bacteriostatic activity, which specifically comprises the following contents:

in a first aspect, the invention provides a traditional Chinese medicine composition with bacteriostatic activity, which comprises the following components in parts by mass: 1-2 parts of scutellaria baicalensis, 1-2 parts of honeysuckle, 1-2 parts of isatis root, 1-2 parts of fructus forsythiae, 1-2 parts of hawthorn, 1-2 parts of mint and 1-2 parts of gallnut.

Preferably, the traditional Chinese medicine composition comprises the following components in parts by mass: 2 parts of scutellaria baicalensis, 1 part of honeysuckle, 2 parts of isatis root, 2 parts of fructus forsythiae, 1 part of hawthorn, 1 part of mint and 2 parts of gallnut.

Preferably, the traditional Chinese medicine composition also comprises the following components in parts by mass: 1-2 parts of schisandra chinensis, 1-2 parts of cinnamon and 1-2 parts of white paeony root.

Preferably, the composition comprises the following components in parts by mass: 2 parts of scutellaria baicalensis, 1 part of honeysuckle, 2 parts of isatis root, 2 parts of fructus forsythiae, 1 part of hawthorn, 1 part of mint, 2 parts of gallnut, 1 part of schisandra chinensis, 1 part of cinnamon and 1 part of radix paeoniae alba.

In a second aspect, the present invention provides a method for preparing the traditional Chinese medicine composition of the first aspect, wherein the method comprises: boiling the Chinese medicinal composition in water; filtering; heating and concentrating the filtrate to obtain the product.

Preferably, the method comprises the steps of:

(1) taking the Chinese medicinal composition, adding ten times of distilled water, heating with strong fire to boil, and heating with slow fire for 1 hr;

(2) filtering, taking the dregs of a decoction, adding ten times of distilled water, and flushing back to the step (1);

(3) filtering, mixing filtrates, heating and concentrating to 1g/mL, standing at room temperature for 24h, centrifuging to obtain supernatant, and autoclaving.

In a third aspect, the invention provides an application of the traditional Chinese medicine composition of the first aspect in preparing antibacterial drugs.

In a fourth aspect, the present invention provides a pharmaceutical acceptable carrier added to the traditional Chinese medicine composition of the first aspect to prepare any pharmaceutically acceptable dosage form.

Preferably, the dosage form comprises tablets, injections, oral liquids and sprays.

In a fifth aspect, the invention provides an application of the traditional Chinese medicine composition in the first aspect in preparing a hand sanitizer, bacteriostatic soap, cosmetics, health food and health products.

The invention has the beneficial effects that: the invention provides a traditional Chinese medicine composition with antibacterial activity, which comprises scutellaria baicalensis, honeysuckle, isatis root, fructus forsythiae, hawthorn, mint and gallnut; secondly, the traditional Chinese medicine composition has obvious bacteriostatic effect on staphylococcus aureus, escherichia coli and candida albicans, and the bacteriostatic effect is better than that of a single traditional Chinese medicine; the dosage of the medicine is reduced, and the toxicity and the bacterial drug resistance are reduced; the traditional Chinese medicine composition has good bacteriostatic effect, high stability, simple operation, low production cost, high extraction rate and convenient production and application; the traditional Chinese medicine composition can be used for preparing disinfection and killing products, including hand sanitizer, spray, bacteriostatic soap and the like with bacteriostatic functions, so that the range and the depth of development and application of traditional Chinese medicines are expanded, meanwhile, bacteriostatic components of the traditional Chinese medicines are applied to the products, and the technology has wide market application prospect and is certainly favored by consumers.

Drawings

The spray prepared in figure 1.

Detailed Description

The following detailed description of the present invention is provided in connection with specific examples, but it should be noted that the present invention is not limited to the following examples. All technical solutions which can be derived from a technical solution by a person skilled in the art through logical analysis, inference and experiment according to the technical solutions of the present invention are within the scope of the claimed invention.

Example 1 detection of bacteriostatic effects of Single Chinese medicine

1. Preparing a single traditional Chinese medicine extract:

weighing 50g of radix Scutellariae, flos Lonicerae, radix Isatidis, fructus forsythiae, fructus crataegi, herba Menthae, Galla chinensis, fructus Schisandrae chinensis, cortex Cinnamomi, and radix Paeoniae alba, respectively, adding into beaker, adding ten times of distilled water, heating with strong fire to boil, heating with slow fire, and stirring for 1 hr; filtering with 8 layers of gauze; taking the medicine residues, and pouring ten times of distilled water, wherein the steps are the same as the above steps; filtering, mixing filtrates, heating and concentrating to 1g/mL, standing at room temperature for 24h, centrifuging at 1000r/min for 5min, collecting supernatant, autoclaving, and storing at 4 deg.C.

2. Zone of inhibition detection

Adopting agar plate punching method, uniformly coating 100 μ L of test bacteria on nutrient agar, standing for 15min, uniformly punching on nutrient agar with a puncher with diameter of 6mm, removing agar block, adding Chinese medicinal or compound extractive solution containing crude drug 1g/mL into holes with liquid medicine overflow principle, 5 holes per plate, hole spacing >20mm, setting distilled water as negative control at center, setting positive control, culturing at 37 deg.C for 24 hr, determining diameter of antibacterial ring, observing result, and repeating the test for 3 times. The bacteriostatic diameter is more than or equal to 20mm, the bacteriostatic diameter is extremely sensitive, the bacteriostatic diameter is 15-19 mm, the bacteriostatic diameter is highly sensitive, the bacteriostatic diameter is 10-14 mm, the bacteriostatic diameter is moderate sensitive, and the bacteriostatic diameter is less than 10mm, the bacteriostatic diameter is low sensitive.

The results are shown in table 1, in the single traditional Chinese medicine, only gallnut, scutellaria baicalensis, honeysuckle and hawthorn have certain bacteriostatic activity on escherichia coli, staphylococcus aureus and candida albicans, and the isatis root, the forsythia suspensa and the mint are not sensitive to the bacteriostatic activity of the escherichia coli, the staphylococcus aureus and the candida albicans.

TABLE 1 diameter of inhibition zone (mm) of different Chinese medicinal aqueous extracts to detection bacteria

MIC and MBC determination

Using a puncher to perform high-pressure sterilization on a circular filter paper sheet of filter paper for later use; preparing a drug stock solution by a test tube double dilution method according to different concentrations of drugs in the circular filter paper sheets, wherein the stock solution concentration is as follows: 500. 250, 125, 62.5, 31.25, 15.62, 7.81 and 3.91mg/mL, and soaking the circular filter paper sheet with the medicine liquid with different concentrations; and (3) taking 100 mu L of test bacterium liquid to a formed blank agar plate, uniformly coating the test bacterium liquid by using a bacterium coating rod, and flatly and sequentially pasting filter paper sheets soaked with different liquid medicine concentrations to the agar plate after the bacterium suspension on the plate is completely absorbed. And culturing the mixture in an incubator which is internally provided with filter paper sheets and is subjected to ultraviolet sterilization at 37 ℃ for 18-24 h within 15min, and reading and measuring the result. Taking the lowest concentration of a drug stock solution which does not generate an inhibition zone around a filter paper sheet (namely, inhibits the growth of a tested bacterium) as the lowest inhibition concentration (MIC) of the drug solution, inoculating the concentration of a culture solution which is generated by the inhibition zone on all K-B paper sheet methods on an agar plate, culturing for 24h at 37 ℃, and taking the lowest drug mass concentration of which the colony count is not more than 5 as the minimum bactericidal mass concentration (MBC) of the drug (namely, the general name of the lowest concentration of the drug for killing a certain strain).

The results are shown in table 2, although the gallnut, the scutellaria baicalensis, the honeysuckle and the hawthorn in the single traditional Chinese medicine have bacteriostatic activity on escherichia coli, staphylococcus aureus and candida albicans below 500mg/mL, the bacteriostatic effect of the other single traditional Chinese medicine is not obvious; but except gallnut, the MBC of other single traditional Chinese medicines to escherichia coli, staphylococcus aureus and candida albicans is higher than 500mg/mL, and the sterilization effect is not obvious.

TABLE 2 MIC and MBC determination results (mg/mL) of single Chinese medicine for different bacteria

Example 2 Chinese medicine formulation screening

1. Preparation of Chinese medicinal composition with different proportions

Chinese medicinal compositions with different proportions are prepared according to the mass ratio shown in the following table 3.

TABLE 3 Mass ratio of the Chinese medicinal materials

	Forsythia fruit	Haw	Radix Isatidis	Galla chinensis	Honeysuckle	Radix Scutellariae	Mint
								Chinese medicinal composition 1	1	1	1	1	1	1	1
Chinese medicinal composition 2	1	1	1	2	2	2	2
								Chinese medicinal composition 3	1	2	2	1	1	2	2
Chinese medicinal composition 4	1	2	2	2	2	1	1
								Chinese medicinal composition 5	2	1	2	1	2	1	2
Chinese medicinal composition 6	2	1	2	2	1	2	1
								Chinese medicinal composition 7	2	2	1	1	2	2	1
Chinese medicinal composition 8	2	2	1	2	1	1	2

2. Results of orthogonal experiments

The results of orthogonal experiments on the antibacterial activity of escherichia coli are shown in table 4, and the antibacterial zone of a traditional Chinese medicine composition (crude drug dose is 1g/mL) prepared by combining scutellaria baicalensis, honeysuckle, isatis root, fructus forsythiae, hawthorn, mint and gallnut according to different proportions is obviously higher than that of a single traditional Chinese medicine (crude drug dose is 1g/mL) of scutellaria baicalensis, honeysuckle, isatis root, fructus forsythiae, hawthorn, mint and gallnut, so that the antibacterial activity of escherichia coli can be obviously inhibited, and the antibacterial activity is obvious.

TABLE 4L 7(28) orthogonal test Table and inhibitory Effect on E.coli

Orthogonal experiment results of the antibacterial activity of the staphylococcus aureus are shown in table 5, the antibacterial zone of a traditional Chinese medicine composition (crude drug dose is 1g/mL) prepared by combining scutellaria baicalensis, honeysuckle, isatis root, fructus forsythiae, hawthorn, mint and gallnut according to different proportions to the staphylococcus aureus is obviously higher than that of a single traditional Chinese medicine (crude drug dose is 1g/mL) prepared from scutellaria baicalensis, honeysuckle, isatis root, fructus forsythiae, hawthorn and mint, the staphylococcus aureus can be obviously inhibited, and the antibacterial activity of the traditional Chinese medicine composition is obvious.

TABLE 5L₇(2⁸) Orthogonal test table and inhibitory effect on staphylococcus aureus

As can be seen from tables 4 and 5, the relationship that each factor affects the antibacterial effect of escherichia coli is a > D > F > G > C > B ═ E; B. e has a weak antibacterial effect on escherichia coli, and A, D has a strong antibacterial effect on escherichia coli, and the antibacterial effect of staphylococcus aureus is influenced by the factors of D > C > F > A > B and E-G. Therefore, B, E has weak bacteriostatic effect on escherichia coli and staphylococcus aureus, and A, D has certain inhibitory effect on escherichia coli and staphylococcus aureus. Through screening, the optimal mixture ratio obtained is A2B1C2D2E1F2G1, namely the mass ratio of fructus forsythiae, hawthorn, radix isatidis, gallnut, honeysuckle, scutellaria baicalensis and mint is 2: 1: 2: 2: 1: 2: 1, namely the traditional Chinese medicine composition 6, has obvious bacteriostatic effect on both escherichia coli and staphylococcus aureus.

MIC and MBC assays

Using a puncher to perform high-pressure sterilization on a circular filter paper sheet of filter paper for later use; preparing a drug stock solution by a test tube double dilution method according to different concentrations of drugs in the circular filter paper sheets, wherein the stock solution concentration is as follows: 500. 250, 125, 62.5, 31.25, 15.62, 7.81 and 3.91mg/mL, and soaking the circular filter paper sheet with the medicine liquid with different concentrations; and (3) taking 100 mu L of test bacterium liquid to a formed blank agar plate, uniformly coating the test bacterium liquid by using a bacterium coating rod, and flatly and sequentially pasting filter paper sheets soaked with different liquid medicine concentrations to the agar plate after the bacterium suspension on the plate is completely absorbed. And culturing the mixture in an incubator which is internally provided with filter paper sheets and is subjected to ultraviolet sterilization at 37 ℃ for 18-24 h within 15min, and reading and measuring the result. Taking the lowest concentration of a drug stock solution which does not generate an inhibition zone around a filter paper sheet (namely, inhibits the growth of a tested bacterium) as the lowest inhibition concentration (MIC) of the drug solution, inoculating the concentration of a culture solution which is generated by the inhibition zone on all K-B paper sheet methods on an agar plate, culturing for 24h at 37 ℃, and taking the lowest drug mass concentration of which the colony count is not more than 5 as the minimum bactericidal mass concentration (MBC) of the drug (namely, the general name of the lowest concentration of the drug for killing a certain strain).

The results are shown in table 6, wherein the mass ratio of fructus forsythiae, hawthorn, radix isatidis, gallnut, honeysuckle, scutellaria baicalensis and mint is 2: 1: 2: 2: 1: 2: 1 (10 mg of radix scutellariae, 5mg of honeysuckle, 10mg of radix isatidis, 10mg of fructus forsythiae, 5mg of hawthorn, 5mg of mint and 10mg of gallnut) has MIC (minimum inhibitory concentration) of 1.95mg/mL and 3.9mg/mL for escherichia coli and staphylococcus aureus; the MBC is 7.81mg/mL and 31.25mg/mL, and the MIC and the MBC are obviously reduced while the antibacterial effect is obvious.

TABLE 6 MIC and MBC determination (mg/mL) of Chinese medicinal composition 6 for different bacteria

Example 3 bacteriostatic activity of the Chinese medicinal composition with addition of fructus Schisandrae chinensis, cortex Cinnamomi, and radix Paeoniae alba

1. Preparation of Chinese medicinal composition with different proportions

The traditional Chinese medicine composition 9: the formula is as follows: 10mg of scutellaria baicalensis, 5mg of honeysuckle, 10mg of isatis root, 10mg of forsythia, 5mg of hawthorn, 5mg of mint, 10mg of gallnut, 10mg of schisandra chinensis, 5mg of cinnamon and 5mg of white paeony root;

10 of the traditional Chinese medicine composition: the formula is as follows: 10mg of scutellaria baicalensis, 5mg of honeysuckle, 10mg of isatis root, 10mg of forsythia, 5mg of hawthorn, 5mg of mint, 10mg of gallnut, 5mg of schisandra chinensis, 5mg of cinnamon and 5mg of white paeony root.

2. Zone of inhibition detection

Adopting agar plate punching method, uniformly coating 100 μ L of test bacteria on nutrient agar, standing for 15min, uniformly punching on nutrient agar with a puncher with diameter of 6mm, removing agar block, adding Chinese medicinal compound extractive solution containing crude drug 1g/mL into holes with liquid medicine overflow principle, 5 holes per plate, hole spacing >20mm, setting distilled water as negative control in the center, setting positive control, culturing at 37 deg.C for 24 hr, determining diameter of antibacterial ring, observing result, and repeating the test for 3 times. The bacteriostatic diameter is more than or equal to 20mm, the bacteriostatic diameter is extremely sensitive, the bacteriostatic diameter is 15-19 mm, the bacteriostatic diameter is highly sensitive, the bacteriostatic diameter is 10-14 mm, the bacteriostatic diameter is moderate sensitive, and the bacteriostatic diameter is less than 10mm, the bacteriostatic diameter is low sensitive.

The results of the zone of inhibition test are shown in table 7, and the traditional Chinese medicine composition formed by adding the schisandra chinensis, the cinnamon and the white paeony root on the basis of the scutellaria baicalensis, the honeysuckle, the isatis root, the forsythia suspensa, the hawthorn, the mint and the gallnut also has a certain inhibition effect, and the effect is equivalent to or better than that of the traditional Chinese medicine composition formed by the scutellaria baicalensis, the honeysuckle, the isatis root, the forsythia suspensa, the hawthorn, the mint and the gallnut.

TABLE 7 zone of inhibition (mm) of Chinese medicinal composition 11, 12 to different bacteria

MIC and MBC determination

Using a puncher to perform high-pressure sterilization on a circular filter paper sheet of filter paper for later use; preparing a drug stock solution by a test tube double dilution method according to different concentrations of drugs in the circular filter paper sheets, wherein the stock solution concentration is as follows: 500. 250, 125, 62.5, 31.25, 15.62, 7.81 and 3.91mg/mL, and soaking the circular filter paper sheet with the medicine liquid with different concentrations; and (3) taking 100 mu L of test bacterium liquid to a formed blank agar plate, uniformly coating the test bacterium liquid by using a bacterium coating rod, and flatly and sequentially pasting filter paper sheets soaked with different liquid medicine concentrations to the agar plate after the bacterium suspension on the plate is completely absorbed. And culturing the mixture in an incubator which is internally provided with filter paper sheets and is subjected to ultraviolet sterilization at 37 ℃ for 18-24 h within 15min, and reading and measuring the result. Taking the lowest concentration of a drug stock solution which does not generate an inhibition zone around a filter paper sheet (namely, inhibits the growth of a tested bacterium) as the lowest inhibition concentration (MIC) of the drug solution, inoculating the concentration of a culture solution which is generated by the inhibition zone on all K-B paper sheet methods on an agar plate, culturing for 24h at 37 ℃, and taking the lowest drug mass concentration of which the colony count is not more than 5 as the minimum bactericidal mass concentration (MBC) of the drug (namely, the general name of the lowest concentration of the drug for killing a certain strain).

The results are shown in table 8, and the traditional Chinese medicine composition formed by adding the schisandra chinensis, the cinnamon and the white paeony root on the basis of the scutellaria baicalensis, the honeysuckle, the isatis root, the fructus forsythiae, the hawthorn, the mint and the gallnut also has a certain antibacterial effect, obviously reduces the MIC and the MBC of a single traditional Chinese medicine while having an obvious antibacterial effect, and has an effect equivalent to or better than that of the traditional Chinese medicine composition formed by the scutellaria baicalensis, the honeysuckle, the isatis root, the fructus forsythiae, the hawthorn, the mint and the gallnut.

TABLE 8 MIC and MBC (mg/mL) of Chinese medicinal compositions 11 and 12 for different bacteria

Comparative example

1. Preparation of Chinese medicinal composition

Comparative composition 1: 10mg of scutellaria baicalensis, 5mg of honeysuckle, 10mg of isatis root and 10mg of gallnut;

comparative composition 2: 10mg of radix Scutellariae, 5mg of flos Lonicerae, and 10mg of Galla chinensis

Comparative composition 3: 10mg of radix scutellariae and 10mg of gallnut;

comparative composition 4: 10mg of scutellaria baicalensis, 5mg of honeysuckle and 10mg of forsythia;

2. zone of inhibition detection

Adopting agar plate punching method, uniformly coating 100 μ L of test bacteria on nutrient agar, standing for 15min, uniformly punching on nutrient agar with a puncher with diameter of 6mm, removing agar block, adding Chinese medicinal compound extractive solution containing crude drug 1g/mL into holes with liquid medicine overflow principle, 5 holes per plate, hole spacing >20mm, setting distilled water as negative control in the center, setting positive control, culturing at 37 deg.C for 24 hr, determining diameter of antibacterial ring, observing result, and repeating the test for 3 times. The bacteriostatic diameter is more than or equal to 20mm, the bacteriostatic diameter is extremely sensitive, the bacteriostatic diameter is 15-19 mm, the bacteriostatic diameter is highly sensitive, the bacteriostatic diameter is 10-14 mm, the bacteriostatic diameter is moderate sensitive, and the bacteriostatic diameter is less than 10mm, the bacteriostatic diameter is low sensitive.

The results of the detection of the inhibition zones are shown in table 9, and although the comparative composition has a certain inhibition effect on escherichia coli and staphylococcus aureus, the inhibition effect is significantly lower than that of the traditional Chinese medicine composition.

TABLE 9 zone of inhibition assay (mm) for comparative compositions

MIC and MBC determination

Using a puncher to perform high-pressure sterilization on a circular filter paper sheet of filter paper for later use; preparing a drug stock solution by a test tube double dilution method according to different concentrations of drugs in the circular filter paper sheets, wherein the stock solution concentration is as follows: 500. 250, 125, 62.5, 31.25, 15.62, 7.81 and 3.91mg/mL, and soaking the circular filter paper sheet with the medicine liquid with different concentrations; and (3) taking 100 mu L of test bacterium liquid to a formed blank agar plate, uniformly coating the test bacterium liquid by using a bacterium coating rod, and flatly and sequentially pasting filter paper sheets soaked with different liquid medicine concentrations to the agar plate after the bacterium suspension on the plate is completely absorbed. And culturing the mixture in an incubator which is internally provided with filter paper sheets and is subjected to ultraviolet sterilization at 37 ℃ for 18-24 h within 15min, and reading and measuring the result. Taking the lowest concentration of a drug stock solution which does not generate an inhibition zone around a filter paper sheet (namely, inhibits the growth of a tested bacterium) as the lowest inhibition concentration (MIC) of the drug solution, inoculating the concentration of a culture solution which is generated by the inhibition zone on all K-B paper sheet methods on an agar plate, culturing for 24h at 37 ℃, and taking the lowest drug mass concentration of which the colony count is not more than 5 as the minimum bactericidal mass concentration (MBC) of the drug (namely, the general name of the lowest concentration of the drug for killing a certain strain).

The results are shown in table 10, and the comparative composition has a certain bacteriostatic effect on escherichia coli and staphylococcus aureus, but the MIC and the MBC of the comparative composition are significantly higher than those of the traditional Chinese medicine composition.

TABLE 10 MIC, MBC (mg/mL) for comparative compositions to different bacteria

EXAMPLE 4 preparation of sprays

1. Preparation process

The formula is as follows: 10mg of radix scutellariae, 5mg of honeysuckle, 10mg of radix isatidis, 10mg of fructus forsythiae, 5mg of hawthorn, 5mg of mint and 10mg of gallnut

In the research of the formulation and the preparation process, the formulation is determined to be a solution type spray, and the types and the dosages of a solvent, a solubilizer, a cosolvent and a preservative are further researched by documents, wherein the test formula is as follows: adding 1% of tween-80, 5% of 1, 2-propylene glycol and 0.2% of benzoic acid into the 75% of alcohol-precipitated traditional Chinese medicine antibacterial solution.

The preparation method of the spray comprises adding benzoic acid into the Chinese medicinal antibacterial liquid under stirring in 40 deg.C water bath, mixing, adding tween-80 and 1, 2-propylene glycol, stirring, filtering, adding distilled water to full volume, and shaking.

The prepared antibacterial spray has natural and safe components, has stronger antibacterial action on escherichia coli, staphylococcus aureus and candida albicans, has light Chinese herbal medicine fragrance, light yellow and clear liquid medicine and fine and uniform aerial fog, is prepared from the traditional Chinese medicine composition with antibacterial activity, has the characteristics and advantages of atomized administration, avoids using a propellant, is safe and reliable, can suspend and stay for a long time, keeps the concentration of the traditional Chinese medicine disinfectant in the air, is particularly suitable for large-area disinfection and sterilization in hospitals, schools and the like, has the disinfection action, can purify air (make dust subside), humidify, scent air and other effects, and is also suitable for daily disinfection of skin and the like.