阅读说明：本技术 一种放线菌和植物共生的培养基质配方及制备工艺 (Formula and preparation process of culture medium for symbiosis of actinomycetes and plants ) 是由 郑殿峰 冯乃杰 沈雪峰 赵黎明 陈观杰 张容郡 于 2021-09-17 设计创作，主要内容包括：本发明公开了一种放线菌和植物共生的培养基质配方,由以下材料的重量份的成分组成：磷酸1－100g、硫酸钾1g-100g、硫酸锌1g-100g、尿素1g-100g、木质素苯磺酸钙1g-100g、NaCl1g-100g、氨水1－100g、7水硫酸镁1g-100g、7水硫酸亚铁1g-100g、褐煤10g-400g、麦饭石10g-400g、沸石粉10g-400g、磷石膏10g-400g、腐殖土10g-400g和硅藻土10g-400g。该放线菌和植物共生的培养基质配方的制备工艺,通过对氨水和褐煤进行搅拌、晾干和磨碎,使得褐煤呈粉状结构,增大氨水与褐煤之间的接触面积,防止氨水的浓度有所改变,通过搅拌使氨水和褐煤混合均匀,避免培养基质出现分层的现象,同时磷酸在与尿素进行反应时通过适当的加温,提高尿素和磷酸反应的速度,减少时间的浪费,同时避免温度过高使磷酸和尿素内部分子发生变化。(The invention discloses a culture medium formula for symbiosis of actinomycetes and plants, which comprises the following components in parts by weight: 1-100 g of phosphoric acid, 1-100 g of potassium sulfate, 1-100 g of zinc sulfate, 1-100 g of urea, 1-100 g of calcium lignosulphonate, 1-100 g of NaCl1g-100g, 1-100 g of ammonia water, 1-100 g of 7-water magnesium sulfate, 1-100 g of 7-water ferrous sulfate, 10-400 g of lignite, 10-400 g of medical stone, 10-400 g of zeolite powder, 10-400 g of phosphogypsum, 10-400 g of humus and 10-400 g of diatomite. This preparation technology of culture medium formula of actinomycete and plant symbiosis, through stirring ammonia water and brown coal, dry and grind for the brown coal is the powdery structure, increase the area of contact between ammonia water and the brown coal, prevent that the concentration of ammonia water from changing to some extent, make ammonia water and brown coal misce bene through the stirring, avoid culture medium phenomenon of layering to appear, phosphoric acid is through appropriate heating when reacting with urea simultaneously, improve the speed of urea and phosphoric acid reaction, reduce the waste of time, avoid the high temperature to make the interior molecule of phosphoric acid and urea change simultaneously.)

1. The formula of the culture medium for symbiosis of actinomycetes and plants is characterized by comprising the following materials in parts by weight: 1-100 g of phosphoric acid, 1-100 g of potassium sulfate, 1-100 g of zinc sulfate, 1-100 g of urea, 1-100 g of calcium lignosulphonate, 1-100 g of NaCl1g-100g, 1-100 g of ammonia water, 1-100 g of 7-water magnesium sulfate, 1-100 g of 7-water ferrous sulfate, 10-400 g of lignite, 10-400 g of medical stone, 10-400 g of zeolite powder, 10-400 g of phosphogypsum, 10-400 g of humus and 10-400 g of diatomite.

2. The formula of the culture medium for actinomycetes and plant symbiosis is characterized by comprising the following components in parts by weight: 50g of phosphoric acid, 50g of potassium sulfate, 50g of zinc sulfate, 50g of urea, 50g of calcium lignosulphonate, 50g of NaCl, 50g, 50g of ammonia water, 50g of 7-water magnesium sulfate, 50g of 7-water ferrous sulfate, 200g of lignite, 200g of medical stone, 200g of zeolite powder, 200g of phosphogypsum, 200g of humus and 200g of diatomite.

3. The formula of the culture medium for actinomycetes and plant symbiosis is characterized by comprising the following components in parts by weight: 75g of phosphoric acid, 75g of potassium sulfate, 75g of zinc sulfate, 75g of urea, 75g of calcium lignosulphonate, 75g of NaCl, 75g of ammonia water, 75g of 7-water magnesium sulfate, 75g of 7-water ferrous sulfate, 300g of lignite, 300g of medical stone, 300g of zeolite powder, 300g of phosphogypsum, 300g of humus and 300g of diatomite.

4. The formula of the culture medium for actinomycetes and plant symbiosis is characterized by comprising the following components in parts by weight: 100g of phosphoric acid, 100g of potassium sulfate, 100g of zinc sulfate, 100g of urea, 100g of calcium lignosulphonate, 100g of NaCl, 100g, 100g of ammonia water, 100g of 7-water magnesium sulfate, 100g of 7-water ferrous sulfate, 400g of lignite, 400g of medical stone, 400g of zeolite powder, 400g of phosphogypsum, 400g of humus and 400g of diatomite.

5. The formula of the culture medium for actinomycetes and plant symbiosis is characterized by comprising the following components in parts by weight: 25g of phosphoric acid, 25g of potassium sulfate, 25g of zinc sulfate, 25g of urea, 25g of calcium lignosulphonate, 25g of NaCl, 25g, 25g of ammonia water, 25g of 7-water magnesium sulfate, 25g of 7-water ferrous sulfate, 100g of lignite, 100g of medical stone, 100g of zeolite powder, 100g of phosphogypsum, 100g of humus and 100g of diatomite.

6. The actinomycete and plant symbiotic culture medium formulation as claimed in claim 1, wherein: the pH value of the obtained actinomycete and plant symbiotic nutrient medium is 5.0-7.5 (depending on the pH value of the soil to be applied).

7. The preparation process of the culture medium formula for actinomycetes and plant symbiosis according to any one of claims 1 to 6, characterized by comprising the following specific steps:

s1, adding ammonia water into the lignite, and stirring, airing and grinding for 3 steps;

s2, putting urea into phosphoric acid to generate a chemical reaction to generate urea phosphate which can be commonly used by plants and microorganisms, stirring, airing and grinding;

s3, uniformly stirring urea phosphate and the rest substances generated by the chemical reaction of the premix formed by mixing ammonia water and lignite with urea and phosphoric acid to obtain a nutrient substrate symbiotic with actinomycetes and plants;

s4, taking a certain amount of nutrient medium symbiotic with actinomycetes and plants, putting the nutrient medium into a container such as a bowl and the like, and sterilizing for later use;

s5, soaking the plant seeds in 0.1% potassium permanganate solution for 10 minutes for disinfection, and blotting the plant seeds with sterile paper for later use;

s6, digging a nutrient medium at the center of the pot on a superclean bench by using a sterile spoon for 4 cm, inoculating actinomycetes to be researched, covering the substrate for 1 cm, sowing sterilized seeds, and covering the substrate for 3 cm;

s7, placing the cultivation pot for inoculation and sowing in an aseptic or ventilated bright environment, pouring sterile water, and culturing while keeping the relative water content at 70-80%.

8. The process for preparing a culture medium formulation for symbiosis of actinomycetes and plants according to claim 7, wherein said culture medium formulation comprises: the stirring time of the ammonia water and the lignite is 0.5h, and the lignite is in a granular structure.

9. The process for preparing a culture medium formulation for symbiosis of actinomycetes and plants according to claim 7, wherein said culture medium formulation comprises: the diameter of the urea is 0.6-1.0mm, and the reaction temperature of the phosphoric acid and the urea is 50 ℃.

10. The process for preparing a culture medium formulation for symbiosis of actinomycetes and plants according to claim 7, wherein said culture medium formulation comprises: the urea and the phosphoric acid are placed in an environment with a constant temperature of 50 ℃, a culture dish used by the culture substrate needs to be disinfected before use, and the concentration of the ammonia water is 25-28%.

Technical Field

The invention relates to the technical field of culture medium for symbiosis of actinomycetes and plants, in particular to a formula and a preparation process of the culture medium for symbiosis of actinomycetes and plants.

Background

The actinomycetes are bacteria, grow in a filamentous shape and are mainly propagated through spores, can be divided into nutritional hypha, aerial hypha and spore silk, are widely distributed in nature and mainly exist in water, air and soil, can produce a large amount of hydrolytic enzymes and metabolites, enables the soil to keep balance, improves the yield of crops, needs to culture a culture medium for symbiosis of the actinomycetes and the plants in order to greatly increase the yield of the plants, and can screen the activity degree in the soil of the actinomycetes through the culture medium for symbiosis of the actinomycetes and the plants so as to improve the fertility degree of the soil;

however, the existing culture medium for symbiosis of actinomycetes and plants has the following problems:

in the preparation process of the culture medium for symbiosis of actinomycetes and plants, a large amount of time is consumed, and partial substances are not completely decomposed, so that the uniformity among the substances is reduced, and the activity of the culture medium is possibly influenced;

aiming at the problems, innovative design is urgently needed on the basis of the original culture medium for symbiosis of actinomycetes and plants.

Disclosure of Invention

The invention aims to provide a formula and a preparation process of a culture medium for symbiosis of actinomycetes and plants, which aims to solve the problems that in the preparation process of the culture medium for symbiosis of actinomycetes and plants, a large amount of time is consumed, partial substances are not completely decomposed, so that the uniformity among the substances is reduced, the activity of the culture medium is possibly influenced, meanwhile, the prepared culture medium has large influence on the temperature, the culture medium prepared under the conditions of different temperatures has different performances, the proper temperature can improve the activity of the culture medium, the molecules in the culture medium can be changed when the temperature is too high, the performance of the culture medium is influenced to a certain extent, the content in soil has a certain difference, the growth and development of plants are influenced, and the substances cannot completely react when the temperature is too low, the reaction speed needs to be increased by adding enzyme, and resources are wasted.

In order to achieve the purpose, the invention provides the following technical scheme: a culture medium formula for symbiosis of actinomycetes and plants comprises the following materials in parts by weight: 1-100 g of phosphoric acid, 1-100 g of potassium sulfate, 1-100 g of zinc sulfate, 1-100 g of urea, 1-100 g of calcium lignosulphonate, 1-100 g of NaCl1g-100g, 1-100 g of ammonia water, 1-100 g of 7-water magnesium sulfate, 1-100 g of 7-water ferrous sulfate, 10-400 g of lignite, 10-400 g of medical stone, 10-400 g of zeolite powder, 10-400 g of phosphogypsum, 10-400 g of humus and 10-400 g of diatomite.

Preferably, the formula of the culture medium for actinomycetes and plant symbiosis comprises the following components in parts by weight: 50g of phosphoric acid, 50g of potassium sulfate, 50g of zinc sulfate, 50g of urea, 50g of calcium lignosulphonate, 50g of NaCl, 50g, 50g of ammonia water, 50g of 7-water magnesium sulfate, 50g of 7-water ferrous sulfate, 200g of lignite, 200g of medical stone, 200g of zeolite powder, 200g of phosphogypsum, 200g of humus and 200g of diatomite.

Preferably, the formula of the culture medium for actinomycetes and plant symbiosis comprises the following components in parts by weight: 75g of phosphoric acid, 75g of potassium sulfate, 75g of zinc sulfate, 75g of urea, 75g of calcium lignosulphonate, 75g of NaCl, 75g of ammonia water, 75g of 7-water magnesium sulfate, 75g of 7-water ferrous sulfate, 300g of lignite, 300g of medical stone, 300g of zeolite powder, 300g of phosphogypsum, 300g of humus and 300g of diatomite.

Preferably, the formula of the culture medium for actinomycetes and plant symbiosis comprises the following components in parts by weight: 100g of phosphoric acid, 100g of potassium sulfate, 100g of zinc sulfate, 100g of urea, 100g of calcium lignosulphonate, 100g of NaCl, 100g, 100g of ammonia water, 100g of 7-water magnesium sulfate, 100g of 7-water ferrous sulfate, 400g of lignite, 400g of medical stone, 400g of zeolite powder, 400g of phosphogypsum, 400g of humus and 400g of diatomite.

Preferably, the formula of the culture medium for actinomycetes and plant symbiosis comprises the following components in parts by weight: 25g of phosphoric acid, 25g of potassium sulfate, 25g of zinc sulfate, 25g of urea, 25g of calcium lignosulphonate, 25g of NaCl, 25g, 25g of ammonia water, 25g of 7-water magnesium sulfate, 25g of 7-water ferrous sulfate, 100g of lignite, 100g of medical stone, 100g of zeolite powder, 100g of phosphogypsum, 100g of humus and 100g of diatomite.

Preferably, the obtained actinomycete and plant symbiotic nutrient substrate has a pH value of 5.0-7.5 (depending on the pH value of soil to be applied).

A preparation process of a culture medium formula for symbiosis of actinomycetes and plants comprises the following specific steps:

s1, adding ammonia water into the lignite, and stirring, airing and grinding for 3 steps;

s2, putting urea into phosphoric acid to generate a chemical reaction to generate urea phosphate which can be commonly used by plants and microorganisms, stirring, airing and grinding;

s3, uniformly stirring urea phosphate and the rest substances generated by the chemical reaction of the premix formed by mixing ammonia water and lignite with urea and phosphoric acid to obtain a nutrient substrate symbiotic with actinomycetes and plants;

s4, taking a certain amount of nutrient medium symbiotic with actinomycetes and plants, putting the nutrient medium into a container such as a bowl and the like, and sterilizing for later use;

s5, soaking the plant seeds in 0.1% potassium permanganate solution for 10 minutes for disinfection, and blotting the plant seeds with sterile paper for later use;

s6, digging a nutrient medium at the center of the pot on a superclean bench by using a sterile spoon for 4 cm, inoculating actinomycetes to be researched, covering the substrate for 1 cm, sowing sterilized seeds, and covering the substrate for 3 cm;

s7, placing the cultivation pot for inoculation and sowing in an aseptic or ventilated bright environment, pouring sterile water, and culturing while keeping the relative water content at 70-80%.

Preferably, the stirring time of the ammonia water and the lignite is 0.5h, and the lignite is in a granular structure.

Preferably, the urea has a diameter of 0.6-1.0mm and the reaction temperature of phosphoric acid and urea is 50 ℃.

Preferably, the urea and the phosphoric acid are placed in an environment with a constant temperature of 50 ℃, a culture dish used for the culture substrate needs to be disinfected before use, and the concentration of the ammonia water is 25% -28%.

Compared with the prior art, the invention has the beneficial effects that: the culture medium formula for symbiosis of actinomycetes and plants and the preparation process thereof are characterized in that ammonia water and lignite are stirred, aired and ground, the lignite is in a powdery structure by grinding, the contact area between the ammonia water and the lignite is increased, the ammonia water and the lignite are uniformly mixed, the condition that the lignite with larger volume exists in the ammonia water is avoided, the water in the lignite can be removed by airing, the ammonia water is prevented from being diluted by the water in the lignite, the concentration of the ammonia water is prevented from being changed, the ammonia water and the lignite are uniformly mixed by stirring, the phenomenon of layering of culture matrixes is avoided, the condition that the same culture matrix has a plurality of different activities is prevented, meanwhile, when phosphoric acid reacts with urea, the reaction speed of urea and phosphoric acid is improved by proper heating, the waste of time is reduced, and the phenomenon that the molecules of phosphoric acid and urea are changed due to overhigh temperature is avoided.

Drawings

FIG. 1 is a schematic view of the preparation process of the present invention.

Detailed Description

The following will clearly and completely describe the technical solutions in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The invention provides a technical scheme that: a culture medium formula for symbiosis of actinomycetes and plants comprises the following materials in parts by weight: 1-100 g of phosphoric acid, 1-100 g of potassium sulfate, 1-100 g of zinc sulfate, 1-100 g of urea, 1-100 g of calcium lignosulphonate, 1-100 g of NaCl1g-100g, 1-100 g of ammonia water, 1-100 g of 7-water magnesium sulfate, 1-100 g of 7-water ferrous sulfate, 10-400 g of lignite, 10-400 g of medical stone, 10-400 g of zeolite powder, 10-400 g of phosphogypsum, 10-400 g of humus and 10-400 g of diatomite.

Example 1

A culture medium formula for symbiosis of actinomycetes and plants comprises the following components in parts by weight: 50g of phosphoric acid, 50g of potassium sulfate, 50g of zinc sulfate, 50g of urea, 50g of calcium lignosulphonate, 50g of NaCl, 50g, 50g of ammonia water, 50g of 7-water magnesium sulfate, 50g of 7-water ferrous sulfate, 200g of lignite, 200g of medical stone, 200g of zeolite powder, 200g of phosphogypsum, 200g of humus and 200g of diatomite;

example 2

A culture medium formula for symbiosis of actinomycetes and plants comprises the following components in parts by weight: 75g of phosphoric acid, 75g of potassium sulfate, 75g of zinc sulfate, 75g of urea, 75g of calcium lignosulphonate, 75g of NaCl, 75g, 75g of ammonia water, 75g of 7-water magnesium sulfate, 75g of 7-water ferrous sulfate, 300g of lignite, 300g of medical stone, 300g of zeolite powder, 300g of phosphogypsum, 300g of humus and 300g of diatomite;

example 3

A culture medium formula for symbiosis of actinomycetes and plants comprises the following components in parts by weight: 100g of phosphoric acid, 100g of potassium sulfate, 100g of zinc sulfate, 100g of urea, 100g of calcium lignosulphonate, 100g of NaCl, 100g, 100g of ammonia water, 100g of 7-water magnesium sulfate, 100g of 7-water ferrous sulfate, 400g of lignite, 400g of medical stone, 400g of zeolite powder, 400g of phosphogypsum, 400g of humus and 400g of diatomite.

Example 4

A culture medium formula for symbiosis of actinomycetes and plants comprises the following components in parts by weight: 25g of phosphoric acid, 25g of potassium sulfate, 25g of zinc sulfate, 25g of urea, 25g of calcium lignosulphonate, 25g of NaCl, 25g, 25g of ammonia water, 25g of 7-water magnesium sulfate, 25g of 7-water ferrous sulfate, 100g of lignite, 100g of medical stone, 100g of zeolite powder, 100g of phosphogypsum, 100g of humus and 100g of diatomite.

The technical scheme provides a preparation process of a culture medium formula for symbiosis of actinomycetes and plants, which comprises the following steps:

the method comprises the following specific steps:

s1, adding ammonia water into the lignite, and stirring, airing and grinding for 3 steps;

s2, putting urea into phosphoric acid to generate a chemical reaction to generate urea phosphate which can be commonly used by plants and microorganisms, stirring, airing and grinding;

s3, uniformly stirring urea phosphate and the rest substances generated by the chemical reaction of the premix formed by mixing ammonia water and lignite with urea and phosphoric acid to obtain a nutrient substrate symbiotic with actinomycetes and plants;

s4, taking a certain amount of nutrient medium symbiotic with actinomycetes and plants, putting the nutrient medium into a container such as a bowl and the like, and sterilizing for later use;

s5, soaking the plant seeds in 0.1% potassium permanganate solution for 10 minutes for disinfection, and blotting the plant seeds with sterile paper for later use;

s6, digging a nutrient medium at the center of the pot on a superclean bench by using a sterile spoon for 4 cm, inoculating actinomycetes to be researched, covering the substrate for 1 cm, sowing sterilized seeds, and covering the substrate for 3 cm;

s7, placing the cultivation pot for inoculation and sowing in an aseptic or ventilated bright environment, pouring sterile water, and culturing while keeping the relative water content at 70-80%.

The stirring time of the ammonia water and the lignite is 0.5h, and the lignite is in a granular structure.

The diameter of the urea is 0.6-1.0mm, and the reaction temperature of the phosphoric acid and the urea is 50 ℃.

The urea and the phosphoric acid are placed in an environment with a constant temperature of 50 ℃, a culture dish used by the culture substrate needs to be disinfected before use, and the concentration of the ammonia water is 25-28%.

Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes in the embodiments and/or modifications of the invention can be made, and equivalents and modifications of some features of the invention can be made without departing from the spirit and scope of the invention.