阅读说明：本技术 一种含有苯噻菌胺和松脂酸铜的杀菌组合物及其应用 (Sterilization composition containing benthiavalicarb isopropyl and copper abietate and application thereof ) 是由 韩小爽 叶进刚 于 2021-08-13 设计创作，主要内容包括：本发明涉及公开了一种含有苯噻菌胺和松脂酸铜的杀菌组合物及其应用,有效成分包括苯噻菌胺和松脂酸铜,苯噻菌胺和松脂酸铜在组合物中的重量份数比为1：20～20：1,苯噻菌胺和松脂酸铜重量总和在组合物中重量百分比为5％～80％。本发明采用苯噻菌胺和松脂酸铜复配,可以扩大杀菌谱,不仅能有效防治卵菌纲引起的霜霉病、晚疫病等真菌病害,还能有效防治细菌性病害,最优选在制备防治黄瓜霜霉病、马铃薯晚疫病药物上的应用,松脂酸铜可迅速启动植物生长系统,增强植物营养生长及生殖生长,对植物有明显的刺激生长作用,为对植物的根、叶、茎、花、果均起到精心的呵护作用,达到的增产增收功效,两组分混配具有较高的协同增效作用,从而大大降低人工和药剂成本。(The invention relates to and discloses a sterilization composition containing benthiavalicarb isopropyl and copper abietate and application thereof, wherein the effective components comprise benthiavalicarb isopropyl and copper abietate, and the weight part ratio of benthiavalicarb isopropyl to copper abietate in the composition is 1: 20-20: 1, the sum of the weight of benthiavalicarb isopropyl and the weight of copper abietate accounts for 5 to 80 percent of the composition. According to the invention, benthiavalicarb isopropyl and copper abietate are compounded, so that the bactericidal spectrum can be expanded, the fungal diseases such as downy mildew and late blight caused by oomycetes can be effectively prevented, bacterial diseases can be effectively prevented, most preferably, the application of the compound pesticide in preparation of the medicines for preventing and treating cucumber downy mildew and potato late blight is realized, the copper abietate can quickly start a plant growth system, the vegetative growth and reproductive growth of plants are enhanced, the obvious growth stimulation effect is realized on the plants, the compound pesticide has a meticulous care effect on roots, leaves, stems, flowers and fruits of the plants, the yield and income increasing effect is achieved, and the two components have a high synergistic effect in mixing, so that the labor and medicament cost is greatly reduced.)

1. The sterilizing composition containing benthiavalicarb isopropyl and copper abietate is characterized by comprising the following components in parts by weight: the effective components of the bactericidal composition comprise benthiavalicarb isopropyl and copper abietate, and the weight part ratio of the benthiavalicarb isopropyl to the copper abietate is 1: 20-20: 1.

2. the bactericidal composition containing benthiavalicarb isopropyl and copper abietate according to claim 1, characterized in that: the weight percentage of the sum of the benthiavalicarb isopropyl and the copper abietate in the bactericidal composition is 5-80%.

3. The bactericidal composition containing benthiavalicarb isopropyl and copper abietate according to claim 1, characterized in that: the weight percentage of the sum of the benthiavalicarb isopropyl and the copper abietate in the bactericidal composition is 10-60%.

4. The bactericidal composition containing benthiavalicarb isopropyl and copper abietate according to claim 1, characterized in that: the bactericidal composition also comprises sophorolipid, and the dosage of the sophorolipid is 1% -15% of the total weight of the bactericidal composition.

5. The bactericidal composition containing benthiavalicarb isopropyl and copper abietate according to claim 1, characterized in that: the bactericidal composition also comprises an auxiliary agent, wherein the auxiliary agent comprises one or more of a filler, a wetting agent, a dispersing agent, a solvent, a surfactant, an emulsifier, a stabilizer, a thickening agent, an antifreezing agent, an anti-settling agent and a defoaming agent.

6. The bactericidal composition containing benthiavalicarb isopropyl and copper abietate according to claim 5, wherein the bactericidal composition comprises the following components in percentage by weight: the bactericidal composition can be prepared into one or more of aqueous suspension, oil suspension, microcapsule suspension, aqueous emulsion, microemulsion, missible oil, paste, wettable powder, water dispersible granules, granules and effervescent tablets.

7. The application of the benthiavalicarb isopropyl and copper abietate-containing bactericidal composition in preventing and treating plant fungi and bacterial diseases according to any one of claims 1 to 6.

8. The application of the bactericidal composition containing benthiavalicarb isopropyl and copper abietate in preventing and treating plant fungi and bacterial diseases according to claim 7 is characterized in that: the fungal diseases comprise downy mildew, epidemic disease, scab and anthracnose of melons; eggplant damping off and damping off; late blight of tomato; late blight of potato.

9. The application of the bactericidal composition containing benthiavalicarb isopropyl and copper abietate in controlling plant fungal diseases according to claim 7 is characterized in that: the bacterial diseases comprise bacterial angular leaf spot of melons, vegetable soft rot, fruit tree canker and bacterial leaf streak of rice.

10. The application of the bactericidal composition containing benthiavalicarb isopropyl and copper abietate in controlling plant fungal diseases according to claim 8 is characterized in that: the cucurbit downy mildew is cucumber downy mildew.

Technical Field

The invention belongs to the technical field of pesticides, and particularly relates to a bactericidal composition containing benthiavalicarb isopropyl and copper abietate and application thereof.

Background

Downy mildew and epidemic diseases are common diseases in the crop planting process, downy mildew and phytophthora blight have strong destructiveness and great harmfulness to host plants, crops are often greatly reduced in yield or even dead after being infected, the quality of the crops and the economic benefit of farmers are seriously influenced, the prevention and treatment of the crops are concerned by scientific researchers of countries in the world, and great market space is provided for developing related prevention and treatment products of the crops. According to the literature report, due to the fact that single pesticide and other unscientific application modes cause downy mildew and epidemic diseases to generate drug resistance to a plurality of commonly used pesticides, the increase of application amount and application times after resistance generation causes increased burden on farmers and increased environmental pollution, and therefore, the mixing of different pesticide varieties becomes one of common methods for preventing and treating agricultural diseases and insect pests. Research and development of high-efficiency, low-toxicity and environment-friendly bactericide pesticide have positive significance for agricultural sustainable development.

Benthiavalicarb-isoproyl (Benthiavalicarb-isoproyl) english name: benthiavalicarb-iso propyl, chemical name: [ [ (6-fluorobenzothiazol-2-yl) -ethylcarbamoyl ] -2-methylpropyl ] carbamic acid isopropyl ester. Benthiavalicarb isopropyl is an amino acid amide bactericide which is developed by Japan combinatorial chemistry and developed together with Bayer, and is mainly used for preventing and treating diseases caused by oomycetes pathogenic bacteria. The benthiavalicarb isopropyl has an action mechanism which is supposed to be a cell wall synthesis inhibitor, has good bactericidal activity on phytophthora, has good inhibitory action on the formation of sporangium and the germination of spores under low concentration, but has no action on the release of zoospores and the movement of the zoospores. The benthiavalicarb isopropyl does not influence the oxidation and synthesis of nucleic acid and protein, and does not influence the function of a phytophthora original serosa; and the test result shows that: the benthiavalicarb-isopropyl has bactericidal activity for preventing and treating potato late blight bacteria with resistance to the benzamide bactericide and melon downy mildew with resistance to strobilurin, and the action mechanisms of the benthiavalicarb-isopropyl and the bactericides are different. The benthiavalicarb isopropyl has strong preventing, treating and penetrating activities, and has good lasting effect and rain wash resistance. In field experiments, late blight of potatoes and tomatoes, downy mildew of grapes and other crops can be effectively controlled at lower doses. The chemical structural formula is as follows:

copper abietate is also known as green emulsion copper or dehydroabietic acid copper, and has the chemical name: (1R,4AR,4BR,10AR) -7-isopropyl-1, 4A-dimethyl-2, 3,4,4B,5,6,10, 10A-octahydrophenanthrene-1-carboxylate, an organic acid copper bactericide prepared by reacting rosin (piment) acid with an inorganic copper salt. The copper abietate is easy to dissolve in benzene, turpentine and other organic solvent, and the product preparation is emulsified oil, which has excellent adhesion, spreading performance, permeability, lasting effect, rain wash resistance, no influence of organic copper ion on temperature and high stability. The copper rosinate is an organic copper preparation with high efficiency, low toxicity and wide bactericidal spectrum, has no residue and no harm to human and livestock, and has double functions of prevention, protection and treatment. When the copper abietate is sprayed on the surface of crops, the leaf surfaces are not easy to burn, the phytotoxicity is not easy to generate, a layer of medicine film with good adhesiveness can be quickly formed, the invasion of pathogenic bacteria is prevented, copper ions are slowly released on the surfaces of the crops, and the medicine film can be solidified and inhibit the synthesis of fungi and bacterial proteins, so that the infection capability is lost and the crops die gradually. The copper abietate has better control effect on bacterial and fungal diseases of various vegetable diseases such as canker, bacterial streak, leaf blight, downy mildew of melons, epidemic disease, scab, anthracnose, bacterial angular leaf spot, damping off, late blight and the like. The structural formula is as follows:

sophorolipid is a glycolipid biosurfactant. The surfactant has the general performances of solubilization, emulsification, wetting, foaming, dispersion, surface tension reduction and the like of the conventional surfactant, has the characteristics of no toxicity, 100% biodegradability, temperature resistance, high salt resistance, wide pH adaptation range, environmental friendliness and the like, and is applied to various fields. Meanwhile, the sophorolipid has antibacterial property, and can inhibit not only bacterial activity but also propagation of pathogenic fungi.

The mixing of different kinds of pesticides is a common method for preventing and treating agricultural diseases and insect pests. By screening the pesticide formula, a reasonable formula is screened, the actual control effect can be effectively improved, the dosage is reduced, the cost is reduced, and the generation of the drug resistance of the plant diseases and insect pests is delayed, so that the method is an important means for agricultural comprehensive treatment. The two active ingredients in the composition have different action mechanisms, and the inventor provides a compound bactericidal composition through experimental formula screening, namely a binary compound bactericidal composition which takes benthiavalicarb-isopropyl and copper abietate as effective ingredients.

CN102293200A discloses a bactericidal composition containing zoxamide and copper abietate, and the bactericidal composition is applied to control of fungal diseases of crops such as vegetables, fruit trees, corns and the like. Benthiavalicarb isopropyl belongs to a protective bactericide and is mostly used in the early stage of morbidity. The pesticide habit of farmers is disease-onset pesticide, so that the sterilization components with prevention and treatment effects are screened for compounding and combining, and the benthiavalicarb isopropyl and the copper abietate are mixed to have obvious synergistic and synergistic effects on the downy mildew of melons and the potato late blight, so that the actual prevention effect is improved, and the pesticide is further researched and completed. The application of the composition of benthiavalicarb isopropyl and copper abietate is not reported at present.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provide a bactericidal composition containing benthiavalicarb-isopropyl and copper abietate, which is efficient, low in toxicity, long in lasting period and capable of effectively preventing and treating agricultural fungal and bacterial diseases.

The invention also aims to provide an application method of the sterilizing composition containing benthiavalicarb isopropyl and copper abietate.

The technical problem to be solved by the invention is realized by the following technical scheme:

the sterilizing composition containing benthiavalicarb isopropyl and copper abietate is characterized by comprising the following components in parts by weight: the effective components of the bactericidal composition comprise benthiavalicarb isopropyl and copper abietate, and the weight part ratio of the benthiavalicarb isopropyl to the copper abietate is 1: 20-20: 1.

moreover, the total weight of the benthiavalicarb isopropyl and the copper abietate in the bactericidal composition is 5-80% by weight.

Moreover, the total weight of the benthiavalicarb isopropyl and the copper abietate in the bactericidal composition is 10-60 wt%.

Moreover, the bactericidal composition also comprises sophorolipid, and the dosage of the sophorolipid is 1% -15% of the total weight of the bactericidal composition.

And the bactericidal composition also comprises an auxiliary agent, wherein the auxiliary agent comprises one or more of a filler, a wetting agent, a dispersing agent, a solvent, a surfactant, an emulsifier, a stabilizer, a thickening agent, an antifreezing agent, an anti-settling agent and a defoaming agent.

In addition, the bactericidal composition can be prepared into one or more of aqueous suspension, oil suspension, microcapsule suspension, aqueous emulsion, microemulsion, missible oil, paste, wettable powder, water dispersible granules, granules and effervescent tablets.

An application of a bactericidal composition containing benthiavalicarb isopropyl and copper abietate in preventing and treating plant fungi and bacterial diseases.

Moreover, the fungal diseases comprise downy mildew, epidemic disease, scab and anthracnose of melons; eggplant damping off and damping off; late blight of tomato; late blight of potato.

Moreover, the bacterial diseases comprise bacterial angular leaf spot of melons, soft rot of vegetables, canker of fruit trees and bacterial leaf streak of rice.

Moreover, the melon downy mildew is cucumber downy mildew.

The invention has the advantages and beneficial effects that:

1. compared with a single agent, the sterilization composition containing benthiavalicarb isopropyl and copper abietate has the advantages that the applicable control object range is increased, the sterilization spectrum is expanded, the sterilization composition can be used for controlling various crop fungal diseases such as downy mildew of melons, epidemic disease, scab, anthracnose, eggplant damping off, tomato late blight, potato late blight and the like, meanwhile, the sterilization composition has a good control effect on various crop bacterial diseases such as bacterial angular leaf spot, soft rot, bacterial streak, canker and the like, and is most preferably applied to the preparation of medicines for controlling cucumber downy mildew and potato late blight.

2. The bactericidal composition containing benthiavalicarb isopropyl and copper abietate has the advantages that the action mechanisms of the two effective components are not completely consistent, the defect that resistance is easy to generate due to long-term single use can be overcome, the service life of the pesticide is prolonged, and the bactericidal composition has important significance for comprehensive treatment of pathogen resistance.

3. According to the sterilization composition containing benthiavalicarb isopropyl and copper abietate, sophorolipid serving as a surfactant is derived from organisms, has good compatibility with the environment, meets the development requirements of green and environment-friendly agriculture, and has antibacterial activity and increased drug effect. In a liquid pesticide preparation, the sophorolipid plays roles of wetting, permeating, dissolving and emulsifying, and in a solid preparation, the sophorolipid mainly plays roles of controlling the stabilization of a dissolving agent suspension during dispersion and spraying, and is beneficial to extrusion granulation during processing, so that the stability of the preparation is ensured, and the efficacy plays a role.

4. According to the bactericidal composition containing benthiavalicarb isopropyl and copper abietate, the control effect of the composition on germs is obviously improved compared with that of a single agent, the using dosage of pesticides is reduced, the frequency of pesticide application is reduced, the cost of pesticide application is reduced, and the adverse effect of the pesticides on the ecological environment is reduced. Compared with a single agent, the composition has the advantages of quick drug effect exertion, long efficacy duration, low use cost, convenient use, time and labor saving, and great economic and social benefits in popularization and application.

5. The bactericidal composition containing benthiavalicarb isopropyl and copper abietate has good prevention, treatment and permeation activities, and can be used as a prevention medicament and a treatment medicament.

Detailed Description

The present invention is further illustrated by the following specific examples, which are intended to be illustrative, not limiting and are not intended to limit the scope of the invention.

Example 1

6% benthiavalicarb isopropyl and copper abietate aqueous suspension

The components and mass percentage are as follows:

composition (I)	Content (wt.)
		Benthiavalicarb isopropyl	1％
Abietic acid copper salt	5％
		Sophorolipid	5％
Lignosulfonic acid sodium salt	4％
		Propylene glycol	3％
Xanthan gum	5％
		Methanol	5％
Potassium hydroxide	1％
		Water (W)	Balance of

The preparation method comprises the following steps: firstly, mixing sophorolipid, sodium lignosulfonate, propylene glycol, xanthan gum, methanol, potassium hydroxide and water, shearing and uniformly mixing in a shearing machine, then adding benthiavalicarb isopropyl and copper abietate, grinding for 2-3 h in a ball grinding machine to enable the particle diameter to be below 5mm, and thus obtaining the 6% benthiavalicarb isopropyl-copper abietate aqueous suspension.

Example 2

10% benthiavalicarb isopropyl/copper abietate aqueous emulsion

The components and mass percentage are as follows:

the preparation method comprises the following steps: firstly, mixing benthiavalicarb isopropyl, copper abietate, trimethylbenzene and sophorolipid together, and dissolving the mixture to form a uniform oil phase. Then mixing part of water and other additives of butylated hydroxytoluene, glycerol, silicone oil and magnesium aluminum silicate together, and dissolving the mixture into a uniform water phase. And then adding the oil phase into the water phase while stirring at a high speed in a reaction kettle, slowly adding water until a phase inversion point is reached, then carrying out high-speed shearing, adding the rest water, and shearing for about 30min to obtain the 10% benthiavalicarb-isopropyl-copper abietate emulsion in water.

Example 3

12% benthiavalicarb isopropyl and copper abietate microemulsion

The components and mass percentage are as follows:

composition (I)	Content (wt.)
		Benthiavalicarb isopropyl	8％
Abietic acid copper salt	4％
		Sophorolipid	12％
Calcium dodecyl benzene sulfonate	6％
		Epoxy chloropropane	3％
Acetic acid	2％
		Water (W)	Balance of

The preparation method comprises the following steps: the benthiavalicarb isopropyl and the copper abietate are completely dissolved by the sophorolipid, then other auxiliary agents of the sophorolipid, calcium dodecyl benzene sulfonate, epichlorohydrin and acetic acid are added to be uniformly mixed, finally water is added to be fully stirred, and the 12 percent benthiavalicarb isopropyl and copper abietate microemulsion is prepared.

Example 4:

60% benthiavalicarb isopropyl and copper abietate water dispersible granule

The components and mass percentage are as follows:

the preparation method comprises the following steps: the benthiavalicarb isopropyl, the copper rosinate and other additives are uniformly mixed, crushed by a superfine jet mill, kneaded, added into a fluidized bed granulation dryer for granulation, drying and screening, and then the 60% benthiavalicarb isopropyl-copper rosinate water dispersible granule is prepared.

Example 5

20% benthiavalicarb isopropyl and copper abietate microcapsule suspending agent

Composition (I)	Content (wt.)
		Benthiavalicarb isopropyl	15％
Abietic acid copper salt	5％
		Sophorolipid	8％
Solvent oil	5％
		Phthalic acid dibutyrate	6％
Castor oil polyoxyethylene ether	5％
		Polycarboxylates	11％
Deionized water	Balance of

The preparation method comprises the following steps: mixing benthiavalicarb isopropyl, copper abietate, a high-molecular capsule wall material and a solvent to dissolve the benthiavalicarb isopropyl, the copper abietate and the solvent into a uniform oil phase, adding the oil phase into a water phase solution containing an emulsifier and a dispersant under a shearing condition, adding mannitol to adjust the pH value to 6.5, supplementing the balance by deionized water, and reacting the two materials at an oil-water interface to form a high-molecular capsule wall, so that the 20% benthiavalicarb isopropyl-copper abietate microcapsule suspending agent can be prepared.

Example 6

16% benthiavalicarb isopropyl and copper abietate aqueous emulsion

The components and mass percentage are as follows:

the preparation method comprises the following steps: firstly, mixing benthiavalicarb isopropyl, copper abietate, trimethylbenzene and sophorolipid together, and dissolving the mixture to form a uniform oil phase. And mixing part of water and other auxiliary agents together, and dissolving to form a uniform water phase. And then adding the oil phase into the water phase while stirring at a high speed in the reaction kettle, slowly adding water until a phase inversion point is reached, then carrying out high-speed shearing, adding the rest water, and shearing for about 30min to obtain the 16% benthiavalicarb-isopropyl-copper abietate emulsion in water.

Example 7:

16% benthiavalicarb isopropyl and copper abietate aqueous emulsion

The components and mass percentage are as follows:

composition (I)	Content (wt.)
		Benthiavalicarb isopropyl	15％
Abietic acid copper salt	1％
		Trimethylbenzene	6％
Dibutylhydroxytoluene	7％
		Glycerol	3％
Silicone oil	3％
		Magnesium aluminum silicate	4％
Water (W)	Balance of

The preparation method comprises the following steps: firstly, mixing benthiavalicarb isopropyl, copper abietate and trimethylbenzene together, and dissolving to form a uniform oil phase. And mixing part of water and other auxiliary agents together, and dissolving to form a uniform water phase. And then adding the oil phase into the water phase while stirring at a high speed in the reaction kettle, slowly adding water until a phase inversion point is reached, then carrying out high-speed shearing, adding the rest water, and shearing for about 30min to obtain the 16% benthiavalicarb-isopropyl-copper abietate emulsion in water. Sophorolipid was not added in this example.

Example 8:

16% benthiavalicarb isopropyl and copper abietate aqueous emulsion

The components and mass percentage are as follows:

composition (I)	Content (wt.)
		Benthiavalicarb isopropyl	15％
Abietic acid copper salt	1％
		Sophorolipid	20％
Trimethylbenzene	2％
		Glycerol	2％
Silicone oil	2％
		Magnesium aluminum silicate	2％
Water (W)	Balance of

The preparation method comprises the following steps: firstly, mixing benthiavalicarb isopropyl, copper abietate, trimethylbenzene and sophorolipid together, and dissolving the mixture to form a uniform oil phase. And mixing part of water and other auxiliary agents together, and dissolving to form a uniform water phase. And then adding the oil phase into the water phase while stirring at a high speed in the reaction kettle, slowly adding water until a phase inversion point is reached, then carrying out high-speed shearing, adding the rest water, and shearing for about 30min to obtain the 16% benthiavalicarb-isopropyl-copper abietate emulsion in water. In this example, sophorolipid was added in excess.

Example 9:

16% benthiavalicarb isopropyl and copper abietate aqueous emulsion

The components and mass percentage are as follows:

composition (I)	Content (wt.)
		Benthiavalicarb isopropyl	15％
Abietic acid copper salt	1％
		Sodium carboxymethylcellulose	5％
Trimethylbenzene	6％
		Glycerol	3％
Silicone oil	3％
		Magnesium aluminum silicate	4％
Water (W)	Balance of

The preparation method comprises the following steps: the benthiavalicarb isopropyl, the copper abietate, the trimethylbenzene and the sodium carboxymethyl cellulose are mixed together and dissolved to form a uniform oil phase. And mixing part of water and other auxiliary agents together, and dissolving to form a uniform water phase. And then adding the oil phase into the water phase while stirring at a high speed in the reaction kettle, slowly adding water until a phase inversion point is reached, then carrying out high-speed shearing, adding the rest water, and shearing for about 30min to obtain the 16% benthiavalicarb-isopropyl-copper abietate emulsion in water. In this example, carboxymethyl cellulose was added as a surfactant.

The results of the product performance tests of the above 9 examples are described in table and text.

As can be seen from the table, in example 7 without adding sophorolipid, the cold storage fluidity and the freeze-thaw fluidity of the product were poor, and it was found that the product added with sophorolipid not only had good normal temperature fluidity and hot storage fluidity, but also had excellent cold storage fluidity and freeze-thaw fluidity, and it was suitable for sterilization in the northern or middle regions where the temperature difference was large, and had good product stability.

In example 8, the sophorolipid is added in an amount of 20% or more and 15% or more, and the sophorolipid is added in an amount of 1 to 15% because the proportion of the lipid material is large, which may affect the stability of the whole system to some extent and may cause problems in the preparation of the product.

In example 9, carboxymethyl cellulose, a surfactant, was added, and the cold storage fluidity and the freeze-thaw fluidity were inferior to those of the product to which sophorolipid was added.

Test example 1: cucumber downy mildew field efficacy test

The test site is located in Liubao village in the quiet sea area of Tianjin city, 15% benthiavalicarb water dispersible granules and 20% copper abietate emulsion are used as a contrast agent in the stage of the cucumber downy mildew, the agents in preparation examples 1-8 adopt a spraying method, 10 agent treatments and 1 blank contrast are set in the test, each treatment is repeated for 3 times, the control effect is investigated once in 3 days, 7 days and 15 days after the application, the number of plants is recorded, the cucumber downy mildew incidence rate is counted, the control effect is calculated, and the test result is shown in Table 1.

Investigation method

Level 0: no scab

Level 1: the lesion area accounts for less than 5% of the whole leaf area;

and 3, level: the lesion area accounts for 6 to 15 percent of the whole leaf area;

and 5, stage: the lesion area accounts for 16-25% of the whole leaf area;

and 7, stage: the lesion area accounts for 26-50% of the whole leaf area;

and 9, stage: the lesion area accounts for more than 50% of the whole leaf area.

And calculating disease indexes and prevention and treatment effects of the treatments according to the survey data.

The disease index is calculated according to a formula, and the calculation result retains the last two decimal points:

TABLE 1 field test results of benthiavalicarb isopropyl and copper abietate combination for controlling cucumber downy mildew

As can be seen from Table 1, formulation examples 1 to 7, benthiavalicarb isopropyl and copper abietate have better control effect on cucumber downy mildew than the control single agent treatment. The method shows that the incidence rate of cucumber downy mildew can be reduced by compounding benthiavalicarb isopropyl and copper abietate, the use dosage of pesticides is reduced, the influence of the pesticides on the environment is reduced, and the pesticide cost is reduced. The bacteriostatic effect of the preparation added with sophorolipid as a surfactant on cucumber downy mildew in example 6 is better than that of the preparation added with a chemical surfactant in example 9 and that of the preparation added with no surfactant in example 7, meanwhile, the active component contents of benthiavalicarb-isopropyl and copper abietate in example 2 are obviously lower than those of the active component in example 6, but the sophorolipid content in example 2 is higher than that of example 6, the effect difference of the two samples on cucumber downy mildew is not obvious, and therefore, the sophorolipid has an obvious synergistic effect on disease control.

Example 10: field drug effect test of potato late blight

The test site is located in Miao village of Miao county of Yutian city in Tangshan province, Hebei province, 15% benthiavalicarb isopropyl water dispersible granule and 20% copper abietate water emulsion are used as reference drugs, the drugs in the preparation examples adopt a spraying method, 8 drug treatments and 1 blank treatment are set in the test, each treatment is repeated for 3 times, the control effect is investigated once in 7 days and 15 days after the drug application, the number of diseased plants is recorded, the incidence rate of potato late blight is counted, the control effect is calculated, and the test result is shown in Table 2.

Investigation method

Level 0: the leaf blade is asymptomatic, and the diseased leaf blade is less than 1%

Level 1: the leaves have water-soaked spots or dark green spots, and the number of diseased leaves is 1-25%;

and 2, stage: the leaves are rotten and brown, and the diseased leaves account for 26-50%;

and 3, level: leaf rot or withering and curling, wherein the diseased leaves account for 51-75%;

4, level: the leaves are rotten or withered and curled, and the diseased leaves account for 76 to 89 percent;

and 5, stage: the diseased leaves account for more than 90% of the total leaves or all plants die.

And calculating disease indexes and prevention and treatment effects of the treatments according to the survey data.

The disease index is calculated according to a formula, and the calculation result retains the last two decimal points:

TABLE 2 field test results of the combination of benthiavalicarb-isopropyl and copper abietate for preventing and treating potato late blight

As can be seen from Table 2, the effects of the compound combination of benthiavalicarb isopropyl and copper abietate on controlling the potato late blight are better than those of the control single agent treatment in the preparation examples 1 to 6. The compound of benthiavalicarb isopropyl and copper abietate can reduce the incidence rate of the potato late blight, the benthiavalicarb isopropyl and the copper abietate have obvious synergistic effect, the use dosage of pesticides can be reduced, and the medicine cost is reduced.

Although the embodiments of the present invention have been disclosed for illustrative purposes, those skilled in the art will appreciate that: various substitutions, changes and modifications are possible without departing from the spirit and scope of the invention and the appended claims, and therefore the scope of the invention is not limited to the embodiments disclosed.