阅读说明：本技术 一种益生菌制剂、菌种筛选装置及筛选方法 (Probiotic preparation, strain screening device and screening method ) 是由 仲延龙 邵兴军 朱慧芬 沈井旺 周娟 马明明 毛日文 于 2021-08-24 设计创作，主要内容包括：本发明公开了一种益生菌制剂、菌种筛选装置,包括益生菌制剂筛选罐、旋转架和打磨架,所述益生菌制剂筛选罐包括罐体、激振器和弹性支架,所述激振器固定安装于罐体底面,所述弹性支架环形等距阵列于罐体底面；所述旋转架包括两组旋转气缸和固定安装于旋转气缸之间的托板,两组旋转气缸对称安装于罐体内壁两侧,所述托架上表面环形等距阵列有益生菌制剂托盘,所述罐体内位于旋转架下方滑动插接有筛板,所述打磨架包括支撑板和固定安装于支撑板上表面的调节杆,在罐体内进行益生菌制剂打磨,去除粘黏,保证益生菌制剂的品质；利用托盘和筛板对益生菌制剂的部分碎裂以及完全粉碎的情况进行筛分,提高生产效率,避免人工分拣产生的二次污染。(The invention discloses a probiotic preparation and strain screening device which comprises a probiotic preparation screening tank, a rotating frame and a polishing frame, wherein the probiotic preparation screening tank comprises a tank body, a vibration exciter and an elastic support, the vibration exciter is fixedly arranged on the bottom surface of the tank body, and the elastic support is annularly and equidistantly arrayed on the bottom surface of the tank body; the rotary frame comprises two groups of rotary cylinders and a supporting plate fixedly arranged between the rotary cylinders, the two groups of rotary cylinders are symmetrically arranged on two sides of the inner wall of the tank body, probiotic preparation trays are annularly arranged on the upper surface of the supporting plate at equal intervals, a sieve plate is inserted into the tank body below the rotary frame in a sliding mode, the polishing frame comprises a supporting plate and an adjusting rod fixedly arranged on the upper surface of the supporting plate, and the probiotic preparation is polished in the tank body to remove viscosity, so that the quality of the probiotic preparation is guaranteed; utilize tray and sieve to sieve the cracked and complete kibbling condition of part of probiotic preparation, improve production efficiency, avoid the secondary pollution that manual sorting produced.)

1. The utility model provides a probiotic preparation, bacterial sieving mechanism, includes probiotic preparation screening jar (1), swivel mount (2) and frame (3) of polishing, its characterized in that: the probiotic preparation screening tank (1) comprises a tank body (101), a vibration exciter (102) and an elastic support (4), wherein the vibration exciter (102) is fixedly arranged on the bottom surface of the tank body (101), and the elastic support (4) is annularly and equidistantly arrayed on the bottom surface of the tank body (101);

the rotary frame (2) comprises two groups of rotary cylinders (201) and supporting plates (202) fixedly arranged between the rotary cylinders (201), through holes (205) are formed in the supporting plates (202) at equal intervals in a ring shape, the two groups of rotary cylinders (201) are symmetrically arranged on two sides of the inner wall of the tank body (101), probiotic preparation trays (203) are arranged on the upper surface of the supporting plates (202) in an annular equal interval manner, and a sieve plate (204) is inserted in the tank body (101) below the rotary frame (2) in a sliding manner;

the utility model discloses a probiotic preparation tank, including jar body (101), the top sets up in jar body (101) polishing frame (3), polishing frame (3) include backup pad (301) and fixed mounting in regulation pole (5) of backup pad (301) upper surface, annular equidistant array has brush board (302) of polishing in backup pad (301), brush board (302) of polishing set up directly over probiotic preparation tray (203).

2. The probiotic preparation and strain screening device of claim 1, wherein: the adjusting rod (5) comprises a lower upright post (501), an upper upright post (502) and an internal thread sleeve (503), the lower upright post (501) is fixedly arranged on the upper surface of the supporting plate (301), one end of the internal thread sleeve (503) is rotatably arranged at the top end of the lower upright post (501), and the other end of the internal thread sleeve (503) is in threaded connection with the bottom end of the upper upright post (502).

3. The probiotic preparation and strain screening device of claim 2, wherein: the utility model discloses a construction method for a pot, including jar body (101) top fixed mounting have a tank deck board (6), slidable mounting has hanger plate (7) in tank deck board (6), go up stand (502) top and rotate through deep groove ball bearing and install in hanger plate (7), rotate and install in tank deck board (6) frock hole (18) have all been seted up to jar body (101) both sides, the same height in position of seting up position and internal thread cover (503) of frock hole (18).

4. The probiotic preparation and strain screening device of claim 1, wherein: tank crown cover board (6) upper surface fixed mounting has electric putter (8), the output rod end of electric putter (8) passes through joint (9) fixed connection in hanger plate (7) upper surface position placed in the middle.

5. The probiotic preparation and strain screening device of claim 1, wherein: elastic support (4) include two sets of connecting plates (401) and spring (402) of fixed connection between two sets of connecting plates (401), and are two sets of there are hollow tube (403) between connecting plate (401) respectively fixed mounting, slidable mounting has bracing piece (404) in hollow tube (403), hollow tube (403) one end fixed mounting is kept away from in bracing piece (404) lower surface in connecting plate (401), and hollow tube (403) and bracing piece (404) run through and install inside spring (402).

6. The probiotic preparation and strain screening device of claim 1, wherein: the utility model discloses a polishing brush board, including polishing brush board (302), pivot (10) and motor support (12), pivot (10) one end extends to backup pad (301) top and fixed mounting has first gear (11), fixed surface installs motor support (12) on backup pad (301), fixed mounting has driving motor (13) in motor support (12), the output shaft fixed mounting of driving motor (13) has second gear (14), second gear (14) and first gear (11) meshing.

7. The probiotic preparation and strain screening device of claim 3, wherein: the tank top cover plate is characterized in that a rectangular through groove (15) is formed in the tank top cover plate (6), guide strips (16) are symmetrically arranged in the rectangular through groove (15), guide blocks (17) are symmetrically arranged on two sides of the hanging plate (7), and the guide blocks (17) are in clearance fit with the guide strips (16).

8. The probiotic preparation and strain screening device of claim 1, wherein: the fixed intercommunication in jar body (101) bottom has bin outlet (19), and jar body (101) one side has seted up the observation window, the outside fixed mounting of observation window has transparent ya keli board (20).

9. A method for screening a probiotic preparation and a strain screening apparatus according to any one of claims 1 to 8, wherein: the screening method comprises the following steps:

s1, feeding, namely feeding a probiotic preparation into the tank body (101), equivalently subpackaging the probiotic preparation into a probiotic preparation tray (203), starting an electric push rod (8) to push the polishing hairbrush plate (302) into the probiotic preparation tray (203), and ensuring that the hairbrush of the polishing hairbrush plate (302) is completely attached to the probiotic preparation;

s2, turning, starting the electric push rod (8) again to lift the grinding frame (3) to the initial height, starting the rotary cylinder (201) to drive the supporting plate (202) to turn over, and turning over the ground probiotic preparation in the sieve plate (204);

s3, starting a vibration exciter (102), and matching with the elastic support (4), driving the tank body (101) to vibrate at a certain frequency to complete screening of different levels of the complete granular probiotic preparation, the cracked probiotic preparation and the powder.

Technical Field

The invention relates to the technical field of probiotic preparation and strain screening, in particular to a probiotic preparation, a strain screening device and a screening method.

Background

The probiotics refer to live bacteria preparation and metabolite thereof which improve the micro-ecological balance of the host to play a beneficial role and achieve the purpose of improving the health level and the health state of the host. To date, probiotics found by scientists can be broadly divided into three broad categories, including:

lactobacillus species (such as lactobacillus acidophilus, lactobacillus casei, lactobacillus jensenii, lactobacillus raman, etc.;

bifidobacterium species (such as bifidobacterium longum, bifidobacterium breve, bifidobacterium ovorans, bifidobacterium thermophilum and the like);

③ gram-positive cocci (such as streptococcus faecalis, lactococcus lactis, intermediate streptococcus, etc.). The probiotics can be directly taken as a food additive to maintain the balance of intestinal flora, hundreds of probiotics health care products have been developed abroad to play a plurality of physiological activity functions. Although the beneficial effects of probiotics on human health are known, the resistance of probiotics is poor, the survival rate in nature is low, most probiotics cannot survive when passing through the stomach due to the low pH value and oxidation-reduction potential of the stomach, and the activity of probiotics is almost lost when the small intestine is affected by bile with high concentration.

When the existing probiotic preparation is produced, the existing probiotic preparation is often made into a blocky structure, and during granulation, the formed particles of the probiotic preparation are sticky, partially cracked, completely crushed and the like, and need to be manually screened and sorted, so that manpower is wasted, and secondary pollution to the probiotic preparation can be caused.

Disclosure of Invention

The invention aims to provide a probiotic preparation, a strain screening device and a screening method, wherein the probiotic preparation is polished in a tank body to remove viscosity, so that the quality of the probiotic preparation is ensured; utilize tray and sieve to sieve the cracked and complete kibbling condition of part of probiotic preparation, improve production efficiency, avoid the secondary pollution that manual sorting produced to solve the problem that proposes among the above-mentioned background art.

In order to achieve the purpose, the invention provides the following technical scheme:

a probiotic preparation and strain screening device comprises a probiotic preparation screening tank, a rotating frame and a grinding frame, wherein the probiotic preparation screening tank comprises a tank body, a vibration exciter and an elastic support, the vibration exciter is fixedly arranged on the bottom surface of the tank body, and the elastic support is annularly and equidistantly arrayed on the bottom surface of the tank body;

the rotary frame comprises two groups of rotary cylinders and a supporting plate fixedly arranged between the rotary cylinders, through holes are formed in the supporting plate at equal intervals in an annular mode, the two groups of rotary cylinders are symmetrically arranged on two sides of the inner wall of the tank body, probiotic preparation trays are arranged on the upper surface of the supporting plate at equal intervals in an annular mode, and a sieve plate is inserted below the rotary frame in the tank body in a sliding mode;

the internal top of jar is arranged in to the frame of polishing, the frame of polishing includes backup pad and fixed mounting in the regulation pole of backup pad upper surface, annular equidistant array has the brush board of polishing in the backup pad, the brush board of polishing sets up directly over probiotic preparation tray.

The adjusting rod comprises a lower upright, an upper upright and an internal thread sleeve, the lower upright is fixedly mounted on the upper surface of the supporting plate, one end of the internal thread sleeve is rotatably mounted at the top end of the lower upright, and the other end of the internal thread sleeve is in threaded connection with the bottom end of the upper upright.

The tank top cover plate is fixedly mounted at the top of the tank body, the hanging plate is mounted in the tank top cover plate in a sliding mode, the top end of the upper stand column is rotatably mounted in the hanging plate through a deep groove ball bearing, the upper stand column is rotatably mounted in the tank top cover plate, tool holes are formed in two sides of the tank body, and the tool holes are formed in the same height as the position of the internal thread sleeve.

The electric push rod is fixedly mounted on the upper surface of the tank top cover plate, and the output rod end of the electric push rod is fixedly connected to the middle position of the upper surface of the hanging plate through a connector.

The elastic support comprises two groups of connecting plates and a spring fixedly connected between the two groups of connecting plates, and is characterized in that a hollow tube is fixedly mounted between the connecting plates respectively, a supporting rod is slidably mounted in the hollow tube, one end of the supporting rod is far away from the lower surface of the hollow tube which is fixedly mounted in the connecting plates, and the hollow tube and the supporting rod are installed inside the spring in a penetrating mode.

Wherein, the brush board top of polishing is placed between two parties and is rotated and install the pivot, pivot one end extends to backup pad top and fixed mounting has first gear, fixed surface installs the motor support in the backup pad, fixed mounting has driving motor in the motor support, driving motor's output shaft fixed mounting has the second gear, second gear and first gear engagement.

The tank top cover plate is internally provided with a rectangular through groove, guide strips are symmetrically arranged in the rectangular through groove, guide blocks are symmetrically arranged on two sides of the hanging plate, and the guide blocks are in clearance fit with the guide strips.

The bottom of the tank body is fixedly communicated with a discharge hole, an observation window is formed in one side of the tank body, and a transparent acrylic plate is fixedly mounted on the outer side of the observation window.

A screening method of a probiotic preparation and strain screening device comprises the following steps:

s1, feeding, namely feeding a probiotic preparation into the tank body, equivalently packaging the probiotic preparation into a probiotic preparation tray, starting an electric push rod to push the polishing hairbrush plate into the probiotic preparation tray, and ensuring that a hairbrush of the polishing hairbrush plate is completely attached to the probiotic preparation;

s2, turning, starting the electric push rod again to lift the polishing frame to the initial height, starting the rotary cylinder to drive the supporting plate to turn over, and turning over the polished probiotic preparation in the sieve plate;

s3, starting a vibration exciter, and matching with the elastic support to drive the tank body to vibrate at a certain frequency, so as to complete screening of different levels of the complete granular probiotic preparation, the cracked probiotic preparation and the powder.

In summary, due to the adoption of the technology, the invention has the beneficial effects that:

according to the invention, the polishing frame is arranged in the tank body, the polishing brush plates with the number corresponding to that of the trays are arranged in the polishing frame, the motor is started, the second gears are meshed with the gears, and the polishing brush plates are driven to polish the probiotic preparation in the trays, so that sticky substances are removed, or the mutual sticking of the probiotic preparation is relieved, the efficiency is high, and under the condition that the treatment environment is more artificial, the secondary pollution to the probiotic preparation is avoided to a certain extent;

according to the invention, a plurality of groups of through holes are arranged in the polishing brush plate in an annular equidistant array, during polishing, splashed powder or partially cracked particles can immediately fall onto the sieve plate, after polishing, the treated probiotic preparation is turned into the sieve plate through the rotary cylinder, the qualified probiotic preparation is reserved by the sieve plate, and the sieve plate adopts a drawing design to quickly take materials.

Drawings

FIG. 1 is a schematic view of a partial three-dimensional structure of an internal view of a can body according to the present invention;

FIG. 2 is a front sectional structural view of the present invention;

FIG. 3 is a schematic side view of the present invention;

FIG. 4 is a schematic top view of the engagement of the first and second gears of the present invention;

FIG. 5 is a schematic view of a partial cross-sectional structure of a grinding frame and an adjusting rod connecting structure in a front view according to the present invention;

fig. 6 is a partially enlarged structural view of a portion a in fig. 2 according to the present invention.

In the figure: 1. a probiotic preparation screening tank; 101. a tank body; 102. a vibration exciter; 2. a rotating frame; 201. a rotating cylinder; 202. a support plate; 203. a probiotic formulation tray; 204. a sieve plate; 3. a polishing frame; 301. a support plate; 302. polishing the brush plate; 4. an elastic support; 401. a connecting plate; 402. a spring; 403. a hollow tube; 404. a support bar; 5. adjusting a rod; 501. a lower upright post; 502. an upper upright post; 503. an internal thread sleeve; 6. a tank top cover plate; 7. a hanger plate; 8. an electric push rod; 9. a joint; 10. a rotating shaft; 11. a first gear; 12. a motor bracket; 13. a drive motor; 14. a second gear; 15. a rectangular through groove; 16. a guide strip; 17. a guide block; 18. a tooling hole; 19. a discharge outlet; 20. transparent acrylic sheet.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings of the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention. Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.

The invention provides a probiotic preparation and strain screening device as shown in fig. 1-6, which comprises a probiotic preparation screening tank 1, a rotating frame 2 and a grinding frame 3, wherein the probiotic preparation screening tank 1 comprises a tank body 101, a vibration exciter 102 and an elastic support 4, the vibration exciter 102 is fixedly arranged on the bottom surface of the tank body 101, the elastic support 4 is annularly and equidistantly arrayed on the bottom surface of the tank body 101, the elastic support 4 comprises two groups of connecting plates 401 and springs 402 fixedly connected between the two groups of connecting plates 401, hollow pipes 403 are respectively and fixedly arranged between the two groups of connecting plates 401, supporting rods 404 are slidably arranged in the hollow pipes 403, one ends of the supporting rods 404, far away from the hollow pipes 403, are fixedly arranged on the inner lower surfaces of the connecting plates 401, and the hollow pipes 403 and the supporting rods 404 are arranged in the springs 402 in a penetrating manner.

The rotating frame 2 comprises two groups of rotating cylinders 201 and a supporting plate 202 fixedly arranged between the rotating cylinders 201, through holes 205 are formed in the supporting plate 202 at equal intervals in a ring shape, the two groups of rotating cylinders 201 are symmetrically arranged on two sides of the inner wall of the tank body 101, probiotic preparation trays 203 are arranged on the upper surface of the supporting plate 202 at equal intervals in a ring shape, and a sieve plate 204 is inserted in the tank body 101 below the rotating frame 2 in a sliding mode;

the polishing frame 3 is arranged at the top in the tank body 101, the polishing frame 3 comprises a support plate 301 and an adjusting rod 5 fixedly arranged on the upper surface of the support plate 301, a polishing brush plate 302 is arranged in the annular equidistant array in the support plate 301, the polishing brush plate 302 is arranged right above a probiotic preparation tray 203, a rotating shaft 10 is rotatably arranged at the center position of the top of the polishing brush plate 302, one end of the rotating shaft 10 extends to the top of the support plate 301 and is fixedly provided with a first gear 11, a motor support 12 is fixedly arranged on the upper surface of the support plate 301, a driving motor 13 is fixedly arranged in the motor support 12, a second gear 14 is fixedly arranged at the output shaft end of the driving motor 13, the second gear 14 is meshed with the first gear 11, the driving motor 13 is started, a plurality of groups of second gears 14 are meshed with a plurality of groups of first gears 11 simultaneously, and the plurality of groups of polishing brush plates 302 are driven to polish the probiotic preparation in the probiotic preparation tray 203 simultaneously, remove sticky substances or remove mutual sticking of the probiotic preparations, has high efficiency, and avoids secondary pollution to the probiotic preparations to a certain extent under the condition that the treatment environment is more artificial.

The adjusting rod 5 comprises a lower upright column 501, an upper upright column 502 and an internal thread sleeve 503, the lower upright column 501 is fixedly arranged on the upper surface of a supporting plate 301, one end of the internal thread sleeve 503 is rotatably arranged at the top end of the lower upright column 501, the other end of the internal thread sleeve 503 is in threaded connection with the bottom end of the upper upright column 502, a tank top cover plate 6 is fixedly arranged at the top of the tank body 101, a hanging plate 7 is arranged in the tank top cover plate 6 in a sliding manner, the top end of the upper upright column 502 is rotatably arranged in the hanging plate 7 through a deep groove ball bearing, tool holes 18 are respectively formed in the two sides of the tank body 101 and rotatably arranged in the tank top cover plate 6, the forming positions of the tool holes 18 are at the same height as the position of the internal thread sleeve 503, during adjustment, a hand is inserted into the tool holes 18 to rotate the internal thread sleeve 503, the upper upright column 502 is driven to lift by utilizing a thread structure, and the gap between the polishing hairbrush plate 302 and the probiotic preparation tray 203 is adjusted, a tray 203 that facilitates adaptation to different probiotic formulations; fixed surface installs electric putter 8 on the 6 upper surfaces of tank deck boards, electric putter 8's output rod end is through 9 fixed connection in the position placed in the middle of hanger plate 7 upper surface of joint, sets up electric putter 8 and not only is convenient for fall the frame of polishing 3 to the processing of polishing probiotic preparation, and utilizes electric putter 8 to promote the frame of polishing 3 to rather than interfering during the stirring.

A rectangular through groove 15 is formed in the tank top cover plate 6, guide strips 16 are symmetrically mounted in the rectangular through groove 15, guide blocks 17 are symmetrically arranged on two sides of the hanging plate 7, the guide blocks 17 are in clearance fit with the guide strips 16, and the stability of the lifting structure of the polishing frame 3 is ensured by utilizing the clearance fit of the guide blocks 17 and the guide strips 16; the fixed intercommunication in jar body 101 bottom has bin outlet 19, and jar body 101 one side has seted up the observation window, observation window outside fixed mounting has transparent ya keli board 20, utilizes transparent ya keli board 20 to be convenient for observe the processing condition of brush board 302 to probiotic preparation of polishing when carrying out the seal installation to jar body 101.

A screening method of a probiotic preparation and strain screening device comprises the following steps:

s1, feeding, namely feeding a probiotic preparation into the tank body 101, equivalently packaging the probiotic preparation into the probiotic preparation tray 203, starting the electric push rod 8 to push the polishing hairbrush plate 302 into the probiotic preparation tray 203, and ensuring that the hairbrush of the polishing hairbrush plate 302 is completely attached to the probiotic preparation;

s2, turning, starting the electric push rod 8 again to lift the grinding frame 3 to the initial height, starting the rotary cylinder 201 to drive the supporting plate 202 to turn over, and turning over the ground probiotic preparation in the sieve plate 204;

s3, starting a vibration exciter 102, and matching with the elastic support 4 to drive the tank body 101 to vibrate at a certain frequency, so as to complete screening of different levels of the complete granular probiotic preparation, the cracked probiotic preparation and the powder.

Example 2

The strains of the probiotics comprise the priority arrangement of numerical values from low to high, and the last bit refers to the beneficial bacterium strain with the lowest content analysis or calculation numerical value; the last three sequencing positions refer to beneficial bacterium strains with the content analysis or calculation values of first to last, second and third positions;

the strain screening step of the probiotics comprises the following steps:

s1, extracting all genes of bacteria contained in the sample;

s2, providing a plurality of beneficial bacterium groups and an internal correction beneficial bacterium group; the beneficial bacterium groups are respectively used for identifying one kind of beneficial bacterium in the sample; the internal correction beneficial bacterium flora is a beneficial bacterium flora used for detecting all kinds of bacterial genes in the sample, and a preposed flora and a reversed flora of the internal correction beneficial bacterium flora; .

S3, carrying out real-time detection polymerase chain reaction on the internal correction beneficial bacterium group and all the genes of the bacteria, thereby obtaining the content of all kinds of bacterial gene products in the sample;

s4, carrying out real-time detection polymerase chain reaction on the beneficial bacterium groups and all genes of the bacteria respectively, thereby obtaining the gene product content of the single beneficial bacterium;

and S5, obtaining single beneficial bacteria after the operation is finished, and completing the analysis of the beneficial bacteria strains.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts of the present invention are equivalent to or changed within the technical scope of the present invention.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.