阅读说明：本技术 龙须菜不饱和脂肪酸保湿护肤品及其制备方法 (Asparagus unsaturated fatty acid moisturizing skin care product and preparation method thereof ) 是由 吕应年 李立 罗辉 于 2021-08-25 设计创作，主要内容包括：本发明公开了一种龙须菜不饱和脂肪酸保湿护肤品及其制备方法,该龙须菜不饱和脂肪酸保湿护肤品为含有龙须菜不饱和脂肪酸的保湿护肤霜或者保湿护肤油或者保湿护肤乳,其中,含有龙须菜不饱和脂肪酸的保湿护肤霜包括以下质量百分比的组分：龙须菜不饱和脂肪酸,1.0％～10.0％；保湿剂,1.6％～4.2％；调理剂,4.0％～6.0％；增稠剂,3.8％～6.2％；表面活性剂,1.0％～3.0％；水,余量。本发明公开的龙须菜不饱和脂肪酸保湿护肤品具有显著的保湿效果且对皮肤温和无刺激。(The invention discloses a asparagus unsaturated fatty acid moisturizing skin care product and a preparation method thereof, the asparagus unsaturated fatty acid moisturizing skin care product is a moisturizing skin care cream or moisturizing skin care oil or moisturizing skin care milk containing asparagus unsaturated fatty acid, wherein the moisturizing skin care cream containing asparagus unsaturated fatty acid comprises the following components in percentage by mass: 1.0 to 10.0 percent of asparagus unsaturated fatty acid; 1.6 to 4.2 percent of humectant; 4.0 to 6.0 percent of conditioner; 3.8 to 6.2 percent of thickening agent; 1.0-3.0% of surfactant; water and the balance. The asparagus unsaturated fatty acid moisturizing skin care product disclosed by the invention has an obvious moisturizing effect and is mild and non-irritant to skin.)

1. The asparagus unsaturated fatty acid moisturizing skin care product is characterized by being a moisturizing skin care cream containing asparagus unsaturated fatty acid, and the moisturizing skin care cream comprises the following components in percentage by mass:

1.0 to 10.0 percent of asparagus unsaturated fatty acid;

1.6 to 4.2 percent of humectant;

4.0 to 6.0 percent of conditioner;

3.8 to 6.2 percent of thickening agent;

1.0-3.0% of surfactant;

water and the balance.

2. The asparagus unsaturated fatty acid moisturizing skin care product as claimed in claim 1, wherein the moisturizing agent comprises the following components in percentage by mass:

1-3% of isopropyl palmitate;

0.3 to 0.6 percent of glycerin;

0.3 to 0.6 percent of propylene glycol.

3. The asparagus unsaturated fatty acid moisturizing skin care product of claim 1, wherein the conditioner is caprylic capric triglyceride;

and/or the thickening agent comprises the following components in percentage by mass:

3.0 to 4.6 percent of synthetic squalane;

0.3 to 0.6 percent of monoglyceride;

0.2 to 0.4 percent of carbomer 940;

0.1 to 0.2 percent of polyacrylamide;

0.1 to 0.2 percent of C13 to C14 isoparaffin;

0.1 to 0.2 percent of laureth-7;

and/or the surfactant comprises the following components in percentage by mass:

0.5 to 1.5 percent of glycerol stearate;

0.5 to 1.5 percent of PEG-100 stearate.

4. The asparagus unsaturated fatty acid moisturizing skin care product is characterized by being asparagus unsaturated fatty acid moisturizing skin care oil containing asparagus unsaturated fatty acid, and the moisturizing skin care oil comprises the following components in percentage by mass:

1.0 to 10.0 percent of asparagus unsaturated fatty acid;

1.5 to 4.5 percent of humectant;

0.1 to 0.5 percent of aromatic;

1.0 to 3.0 percent of thickening agent;

0.01 to 0.06 percent of antioxidant;

a conditioning agent and the balance.

5. The asparagus unsaturated fatty acid moisturizing skin care product according to claim 4, wherein the moisturizing agent is avocado oil;

and/or the aromatic is rose essential oil;

and/or the thickener is squalane;

and/or the antioxidant is dibutyl hydroxy toluene;

and/or the conditioning agent comprises the following components in percentage by mass:

1.0 to 4.0 percent of olive oil;

caprylic acid/capric acid triglyceride, and the balance.

6. The asparagus unsaturated fatty acid moisturizing skin care product is characterized by being a moisturizing skin care emulsion containing asparagus unsaturated fatty acid, and the moisturizing skin care emulsion comprises the following components in percentage by mass:

1.0 to 10.0 percent of asparagus unsaturated fatty acid;

humectant 4.0-6.2%;

4.0 to 6.0 percent of conditioner;

0.06-0.12% of chelating agent;

4.0 to 6.0 percent of thickening agent;

2.0 to 4.0 percent of lubricant;

2.0-4.0% of functional auxiliary agent;

water and the balance.

7. The asparagus unsaturated fatty acid moisturizing skin care product as claimed in claim 6, wherein the moisturizing agent comprises the following components in percentage by mass:

0.6 to 1.2 percent of gupuacu fruit oil;

0.2 to 0.4 percent of vitamin E acetate;

3.2 to 4.6 percent of glycerin;

and/or the conditioning agent comprises the following components in percentage by mass:

3.6 to 5.2 percent of caprylic acid/capric acid triglyceride;

0.2 to 0.4 percent of D-panthenol;

0.2 to 0.4 percent of allantoin;

and/or the chelating agent is disodium ethylene diamine tetraacetate;

and/or the thickening agent comprises the following components in percentage by mass:

cetostearyl alcohol, 0.6% -1.4%;

3.2 to 4.2 percent of synthetic squalane;

0.1 to 0.4 percent of xanthan gum;

and/or the lubricant is dimethyl silicone oil with the viscosity of 100 cs;

and/or the functional auxiliary agent comprises the following components in percentage by mass:

0.01 to 0.5 percent of aromatic;

0.3 to 1.0 percent of preservative;

1.6 to 2.5 percent of surfactant.

8. A process for preparing a asparagus unsaturated fatty acid moisturizing skin care product according to any of claims 1 to 3, comprising the steps of:

heating and dissolving the phase A component at a heating temperature of 75-85 ℃ for later use; wherein the phase A component comprises caprylic capric triglyceride, synthetic squalane, glyceryl stearate, PEG-100 stearate, monoglyceride, isopropyl palmitate, cetostearyl alcohol and unsaturated fatty acid of Gracilaria verrucosa;

heating and dissolving the phase B component under the heating condition that the heating temperature is 85-95 ℃, adding the dissolved phase A component into the dissolved phase B component, homogenizing for 2-4 min, and cooling to room temperature to obtain the asparagus unsaturated fatty acid-containing moisturizing skin cream; wherein the phase B component comprises glycerol, propylene glycol, carbomer 940, polyacrylamide, C13-C14 isoparaffin, laureth-7 and water.

9. A process for preparing a asparagus unsaturated fatty acid moisturizing skin care product according to any of claims 4 to 5, comprising the steps of:

sequentially adding the components in the moisturizing and skin-care oil into a container, and uniformly stirring until the components are transparent and have no insoluble substances to obtain transparent liquid; wherein each component in the moisturizing and skin-care oil comprises caprylic/capric triglyceride, dibutyl hydroxy toluene, avocado oil, olive oil, squalane, rose essential oil and unsaturated fatty acid of asparagus;

and filtering the transparent liquid by using a filter cloth to obtain the asparagus unsaturated fatty acid-containing moisturizing skin care oil.

10. A process for preparing a asparagus unsaturated fatty acid moisturizing skin care product according to any of claims 6 to 7, comprising the steps of:

heating and dissolving the phase A component at a heating temperature of 75-85 ℃ for later use; wherein the phase A component comprises C16-C18 alkyl glycoside, cetostearyl alcohol, synthetic squalane, caprylic/capric triglyceride, Cupuacu fruit oil, vitamin E acetate, asparagus unsaturated fatty acid and dimethyl silicone oil with the viscosity of 100 cs;

heating and dissolving the phase B component under the heating condition that the heating temperature is 85-95 ℃, adding the dissolved phase A component into the dissolved phase B component, and homogenizing for 2-4 min to obtain a mixture of the phase A component and the phase B component; the phase B component comprises disodium ethylene diamine tetraacetate, glycerol, xanthan gum, D-panthenol, allantoin and water, wherein the xanthan gum is pre-dispersed by the glycerol;

stirring the mixture, cooling, adding a C-phase component into the mixture after the mixture is cooled to 43-46 ℃, continuously stirring, cooling, and cooling to room temperature to obtain the unsaturated fatty acid asparagus moisturizing skin care milk; wherein, the phase C component comprises essence and preservative.

Technical Field

The invention belongs to the technical field of skin care products, and particularly relates to a asparagus unsaturated fatty acid moisturizing skin care product and a preparation method thereof.

Background

In recent years, cosmeceuticals derived from seaweeds have been rapidly developed, and various high-end luxury cosmetics are highly favored by consumers because they are rich in precious seaweed active ingredients. Although some of the compounds have been used in the cosmetic field, there are still a large number of resources to be developed. In marine life, seaweed is recognized as an underdeveloped source of plant resources and cosmetic ingredients. The marine bioactive substances are very popular with people due to their unique skin-friendly property and high biological safety, so that the search for efficient and safe natural whitening agents or moisturizers in marine animals, plants and microorganisms becomes a hot point for research in the pharmaceutical and cosmetic industries.

Gracilaria lemaneiformis is temperate red algae and is mainly distributed in coastal areas such as Shandong, Fujian and Guangdong. In recent years, researches on asparagus have mostly focused on saccharides such as asparagus polysaccharide and asparagus oligosaccharide, agar prepared from asparagus, and the like, and no researches or reports on application of unsaturated fatty acid components in asparagus to skin care products or cosmetics have been found.

The above-mentioned contents are only the techniques known by the inventor, and are only used for assisting understanding of the technical scheme of the invention, and do not represent an admission that the above-mentioned contents are the prior art.

Disclosure of Invention

The invention mainly aims to provide a asparagus unsaturated fatty acid moisturizing skin care product which has an obvious moisturizing effect and is mild and non-irritant to skin.

In order to achieve the purpose, the invention provides a asparagus unsaturated fatty acid moisturizing skin care product, which is a moisturizing skin care cream containing asparagus unsaturated fatty acid, and the moisturizing skin care cream comprises the following components in percentage by mass:

1.0 to 10.0 percent of asparagus unsaturated fatty acid;

1.6 to 4.2 percent of humectant;

4.0 to 6.0 percent of conditioner;

3.8 to 6.2 percent of thickening agent;

1.0-3.0% of surfactant;

water and the balance.

Further, the humectant comprises the following components in percentage by mass:

1-3% of isopropyl palmitate;

0.3 to 0.6 percent of glycerin;

0.3 to 0.6 percent of propylene glycol.

Further, the conditioner is caprylic capric triglyceride.

Further, the thickening agent comprises the following components in percentage by mass:

3.0 to 4.6 percent of synthetic squalane;

0.3 to 0.6 percent of monoglyceride;

0.2 to 0.4 percent of carbomer 940;

0.1 to 0.2 percent of polyacrylamide;

0.1 to 0.2 percent of C13 to C14 isoparaffin;

0.1 to 0.2 percent of laureth-7.

Further, the surfactant comprises the following components in percentage by mass:

0.5 to 1.5 percent of glycerol stearate;

0.5 to 1.5 percent of PEG-100 stearate.

In order to achieve the above objects, the present invention also provides a method for preparing the above moisturizing skin cream containing unsaturated fatty acids of asparagus, comprising the steps of:

heating and dissolving the phase A component at a heating temperature of 75-85 ℃ for later use; wherein the phase A component comprises caprylic capric triglyceride, synthetic squalane, glyceryl stearate, PEG-100 stearate, monoglyceride, isopropyl palmitate, cetostearyl alcohol and unsaturated fatty acid of Gracilaria verrucosa;

heating and dissolving the phase B component under the heating condition that the heating temperature is 85-95 ℃, adding the dissolved phase A component into the dissolved phase B component, homogenizing for 2-4 min, and cooling to room temperature to obtain the asparagus unsaturated fatty acid-containing moisturizing skin cream; wherein the phase B component comprises glycerol, propylene glycol, carbomer 940, polyacrylamide, C13-C14 isoparaffin, laureth-7 and water.

In order to achieve the purpose, the invention also provides a asparagus unsaturated fatty acid moisturizing skin care product, which is moisturizing skin care oil containing asparagus unsaturated fatty acid, and the moisturizing skin care oil comprises the following components in percentage by mass:

1.0 to 10.0 percent of asparagus unsaturated fatty acid;

1.5 to 4.5 percent of humectant;

0.1 to 0.5 percent of aromatic;

1.0 to 3.0 percent of thickening agent;

0.01 to 0.06 percent of antioxidant;

a conditioning agent and the balance.

Further, the humectant is avocado oil.

Further, the aromatic is rose essential oil.

Further, the thickener is squalane.

Further, the antioxidant is dibutyl hydroxy toluene.

Further, the conditioner comprises the following components in percentage by mass:

1.0 to 4.0 percent of olive oil;

caprylic acid/capric acid triglyceride, and the balance.

In order to achieve the above objects, the present invention also provides a method for preparing the above moisturizing skin care oil containing unsaturated fatty acids of asparagus, comprising the steps of:

sequentially adding the components in the moisturizing and skin-care oil into a container, and uniformly stirring until the components are transparent and have no insoluble substances to obtain transparent liquid; wherein each component in the moisturizing and skin-care oil comprises caprylic/capric triglyceride, dibutyl hydroxy toluene, avocado oil, olive oil, squalane, rose essential oil and unsaturated fatty acid of asparagus;

and filtering the transparent liquid by using a filter cloth to obtain the asparagus unsaturated fatty acid-containing moisturizing skin care oil.

In order to achieve the purpose, the invention also provides a asparagus unsaturated fatty acid moisturizing skin care product, which is a moisturizing skin care emulsion containing asparagus unsaturated fatty acid, and the moisturizing skin care emulsion comprises the following components in percentage by mass:

1.0 to 10.0 percent of asparagus unsaturated fatty acid;

humectant 4.0-6.2%;

4.0 to 6.0 percent of conditioner;

0.06-0.12% of chelating agent;

4.0 to 6.0 percent of thickening agent;

2.0 to 4.0 percent of lubricant;

2.0-4.0% of functional auxiliary agent;

water and the balance.

Further, the humectant comprises the following components in percentage by mass:

0.6 to 1.2 percent of gupuacu fruit oil;

0.2 to 0.4 percent of vitamin E acetate;

3.2 to 4.6 percent of glycerin.

Further, the conditioner comprises the following components in percentage by mass:

3.6 to 5.2 percent of caprylic acid/capric acid triglyceride;

0.2 to 0.4 percent of D-panthenol;

0.2 to 0.4 percent of allantoin.

Further, the chelating agent is disodium ethylene diamine tetraacetate.

Further, the thickening agent comprises the following components in percentage by mass:

cetostearyl alcohol, 0.6% -1.4%;

3.2 to 4.2 percent of synthetic squalane;

0.1 to 0.4 percent of xanthan gum.

Further, the lubricant is simethicone with the viscosity of 100 cs.

Further, the functional auxiliary agent comprises the following components in percentage by mass:

0.01 to 0.5 percent of aromatic;

0.3 to 1.0 percent of preservative;

1.6 to 2.5 percent of surfactant.

Further, the aromatic is essence.

Further, the surfactant is C16-C18 alkyl glycoside.

In order to achieve the above object, the present invention also provides a method for preparing the above moisture-keeping skin care milk containing unsaturated fatty acids of asparagus, comprising the steps of:

heating and dissolving the phase A component at a heating temperature of 75-85 ℃ for later use; wherein the phase A component comprises C16-C18 alkyl glycoside, cetostearyl alcohol, synthetic squalane, caprylic/capric triglyceride, Cupuacu fruit oil, vitamin E acetate, asparagus unsaturated fatty acid and dimethyl silicone oil with the viscosity of 100 cs;

heating and dissolving the phase B component under the heating condition that the heating temperature is 85-95 ℃, adding the dissolved phase A component into the dissolved phase B component, and homogenizing for 2-4 min to obtain a mixture of the phase A component and the phase B component; the phase B component comprises disodium ethylene diamine tetraacetate, glycerol, xanthan gum, D-panthenol, allantoin and water, wherein the xanthan gum is pre-dispersed by the glycerol;

stirring the mixture, cooling, adding a C-phase component into the mixture after the mixture is cooled to 43-46 ℃, continuously stirring, cooling, and cooling to room temperature to obtain the unsaturated fatty acid asparagus moisturizing skin care milk; wherein, the phase C component comprises essence and preservative.

Compared with the prior art, the invention has the beneficial effects that:

the asparagus unsaturated fatty acid moisturizing skin care product provided by the invention takes asparagus unsaturated fatty acid as a main component, and through the change of a formula and a production process, the moisturizing skin care product can be moisturizing skin care cream, moisturizing skin care oil or moisturizing skin care milk; and because the asparagus unsaturated fatty acid is derived from asparagus which can be used as food and is a safe and nontoxic natural plant component, the asparagus unsaturated fatty acid moisturizing skin care product provided by the invention not only has an obvious moisturizing effect, but also is mild and non-irritant to skin, and is suitable for most people to use.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the structures shown in the drawings without creative efforts.

FIG. 1 is a comparison graph of water loss rates of an experimental group and a control group with time under a color-changing silica gel environment according to an embodiment of the present invention;

FIG. 2 is a graph showing a comparison of water loss rates of an experimental group and a control group over time in an environment with a relative humidity of 34% according to an embodiment of the present invention;

FIG. 3 is a comparison of the skin moisture rate of change over time between the experimental group and the control group in accordance with an embodiment of the present invention.

The implementation, functional features and advantages of the objects of the present invention will be further explained with reference to the accompanying drawings.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Firstly, the asparagus unsaturated fatty acid in the asparagus is extracted by the following method:

(1) weighing a proper amount (such as 150 g) of asparagus dry powder, and putting the asparagus dry powder into a first container (such as a round-bottom flask);

(2) adding ethanol with the concentration of 70% into the first container, and carrying out ultrasonic treatment for 18-22 min; wherein the feed-liquid ratio of the dry asparagus powder to the ethanol is 1: 10;

(3) heating the first container to 78-82 ℃, refluxing for 2h, and transferring the obtained mixture into a second container (such as a rotary evaporation bottle);

(4) distilling the mixture under reduced pressure to remove ethanol in the mixture to obtain a crude extract;

(5) adding 3 times of deionized water to dissolve and dilute the crude extract to obtain a diluent;

(6) adding an isovolumetric petroleum ether ethyl acetate mixture (1:1) into the diluent for extracting unsaturated fatty acid substances in the diluent, repeatedly extracting for three times, and combining organic phases to obtain an extract;

(7) distilling the extract under reduced pressure to remove the petroleum ether ethyl acetate in the extract, wherein the obtained residual oily matter is the unsaturated fatty acid of the asparagus; wherein the unsaturated fatty acid of thallus Gracilariae mainly comprises unsaturated fatty acid components such as hexadecanoic acid (38.57%), oleic acid (25.48%), arachidonic acid (12.84%), etc.

In addition, practice shows that the extraction rate of the asparagus unsaturated fatty acid in the asparagus extracted by the method can reach 4% (4 g of the asparagus unsaturated fatty acid can be extracted from 100 g of asparagus dry powder).

Next, preferred examples of the present invention will be described in detail, and experimental methods in which specific conditions are not specified in the examples are generally conventional conditions.

Example 1

The moisturizing skin cream containing the asparagus unsaturated fatty acid comprises the following components in percentage by mass (see table 1).

TABLE 1 moisturizing skin cream formulations

The preparation method of the moisturizing skin cream containing the asparagus unsaturated fatty acid comprises the following steps:

step S11, heating and dissolving the phase A component under the heating condition that the heating temperature is 75-85 ℃ for later use; wherein, the phase A component comprises caprylic capric triglyceride, synthetic squalane, glyceryl stearate, PEG-100 stearate, monoglyceride, isopropyl palmitate, cetostearyl alcohol and unsaturated fatty acid of Gracilaria verrucosa;

step S12, heating and dissolving the phase B component under the heating condition that the heating temperature is 85-95 ℃, adding the dissolved phase A component into the dissolved phase B component, homogenizing for 2-4 min, and cooling to room temperature to obtain the asparagus unsaturated fatty acid-containing moisturizing skin cream; wherein, the phase B component comprises glycerol, propylene glycol, carbomer 940, polyacrylamide, C13-C14 isoparaffin, laureth-7 and water.

Example 2

The moisturizing skin care oil containing the unsaturated fatty acid of the asparagus comprises the following components in percentage by mass (see table 2).

TABLE 2 moisturizing skin care oil formulation

Name of raw materials	Mass fraction/%	Use of raw materials
			Caprylic/capric triglyceride	The balance (to 100)	Conditioning agent
Dibutylhydroxytoluene	0.01～0.06	Antioxidant agent
			Avocado oil	1.5～4.5	Moisture-retaining agent
Olive oil	1.0～4.0	Conditioning agent
			Squalane	1.0～3.0	Thickening agent
Rose essential oil	0.1～0.5	Aromatic agent
			Unsaturated fatty acid of asparagus	1.0～10.0	Moisture-retaining agent

The preparation method of the moisturizing skin care oil containing the asparagus unsaturated fatty acid comprises the following steps:

step S21, sequentially adding the components in the table 2 into a container (such as a main pot), and uniformly stirring until the mixture is transparent and has no insoluble substances to obtain transparent liquid;

and step S22, filtering the transparent liquid by using filter cloth, and filtering to obtain the asparagus unsaturated fatty acid-containing moisturizing skin care oil.

Example 3

The moisturizing skin care lotion containing the unsaturated fatty acid of asparagus comprises the following components in percentage by mass (see table 3).

TABLE 3 moisturizing skin care lotion formulas

The preparation method of the moisturizing and skin-care milk containing the asparagus unsaturated fatty acid comprises the following steps:

step S31, heating and dissolving the phase A component under the heating condition that the heating temperature is 75-85 ℃ for later use; wherein, the phase A component comprises C16-C18 alkyl glycoside, cetostearyl alcohol, synthetic squalane, caprylic/capric triglyceride, Cupuacu fruit oil, vitamin E acetate, asparagus unsaturated fatty acid and simethicone with the viscosity of 100 cs;

step S32, heating and dissolving the phase B component under the heating condition that the heating temperature is 85-95 ℃, adding the dissolved phase A component into the dissolved phase B component, and homogenizing for 2-4 min to obtain a mixture of the phase A component and the phase B component; the phase B component comprises disodium ethylene diamine tetraacetate, glycerol, xanthan gum, D-panthenol, allantoin and water, wherein the xanthan gum is pre-dispersed with the glycerol;

step S33, stirring and cooling the mixture, adding the C-phase component into the mixture after the mixture is cooled to 43-46 ℃, continuing stirring and cooling, and cooling to room temperature to obtain the unsaturated fatty acid asparagus moisturizing skin care milk; wherein, the phase C component comprises essence and preservative.

Example 4

In-vitro moisture-keeping capacity determination of unsaturated fatty acid of asparagus:

the moisturizing skin cream containing the components shown in table 1 (i.e., the moisturizing skin cream containing unsaturated fatty acids of asparagus in example 1) was used as an experimental group, and the general skin cream containing no unsaturated fatty acids of asparagus was used as a blank control group. Wherein, except that the asparagus unsaturated fatty acid is not contained, the formula and the preparation method of the common skin cream of the blank control group are completely consistent with the formula and the preparation method of the moisturizing skin cream containing the asparagus unsaturated fatty acid in the example 1. Specifically, the method comprises the following steps:

preparing 4 portions of the plexiglass plate with the medical breathable adhesive tape stuck thereto according to the same operation (sticking the medical breathable adhesive tape on the plexiglass plate to simulate the skin);

accurately weighing 1.5g of each 2 parts of moisturizing skin cream containing unsaturated fatty acid of asparagus and accurately weighing 1.5g of common skin cream containing no unsaturated fatty acid of asparagus;

2 parts of moisturizing skin cream and 2 parts of common skin cream are respectively and uniformly smeared on a 5cm multiplied by 5cm medical breathable adhesive tape on a corresponding organic glass plate by wearing a latex finger cot by hands;

respectively placing one of the organic glass plates coated with the moisturizing skin cream and one of the organic glass plates coated with the common skin cream in a drier with allochroic silica gel as a drying agent, respectively placing the other of the organic glass plates coated with the moisturizing skin cream and the other of the organic glass plates coated with the common skin cream in a drier with saturated calcium chloride solution (with relative humidity of 34%) for a period of time (1, 2, 3, 4, 6, 8, 9, 10, 11 and 12 hours), accurately weighing the mass of each test sample, and respectively calculating the water loss rate of each test sample along with the change of time. Wherein the temperature in each drier is 20 ℃; before use, the allochroic silica gel in the dryer is placed in an air-blast drying oven with the temperature of 90 ℃ for drying for 10 hours; placing the beaker filled with saturated calcium chloride solution in a dryer 12h in advance; water loss rate (M)₀－M_t)×100％/M₁In the formula, M₀The total mass (g) of the organic glass plate, the medical breathable adhesive tape and the sample (moisturizing skin cream or common skin cream) before drying; m_tTotal mass (g) of the plexiglass plate, medical permeable tape and sample after a set time for drying; m₁Is the mass (g) of the sample applied before the dryer. And (3) performing data analysis by using SPSS 12.0 statistical software, and considering that the difference is significant if the water loss rate P is less than 0.05.

As can be seen from FIG. 1, in the environment of the color-changing silica gel, except at 1h, the water loss rate of the control group is greater than that of the experimental group (P < 0.05) at any other time point, thereby indicating that the unsaturated fatty acid of asparagus can increase the moisturizing performance of the moisturizing cream.

As can be seen from fig. 2, in an environment with a relative humidity of 34%, similar to the allochroic silica gel environment, except at 1h, the water loss rate of the control group is greater than that of the experimental group (P < 0.05) at any other time point, thereby further proving that the moisturizing skin cream containing unsaturated fatty acid of asparagus has a significant moisturizing effect.

Example 5

Influence of unsaturated fatty acid of asparagus on moisture change rate of human skin (test of in vivo moisture retention capacity of unsaturated fatty acid of asparagus):

the influence of the moisturizing skin cream containing unsaturated fatty acids of asparagus in the above example 1 on the rate of change of moisture of human skin was determined by a human body method, and a normal skin cream containing no unsaturated fatty acids of asparagus was used as a blank control. Wherein, except that the asparagus unsaturated fatty acid is not contained, the formula and the preparation method of the common skin cream of the blank control group are completely consistent with the formula and the preparation method of the moisturizing skin cream containing the asparagus unsaturated fatty acid in the example 1. Specifically, the method comprises the following steps:

25 healthy female volunteers without skin diseases are selected, aged between 18 and 25 years, and tested by a CM825 type skin moisture tester under the conditions that the ambient temperature is 22 ℃ and the relative humidity is 60 percent. Any cosmetics or external medicines can not be used for 2 to 3 days before the test of the tested part, and water can not be contacted for 1 to 3 hours before the test. Before the test, the testee needs to clean the inner sides of the forearms of the hands uniformly, and the cleaning method is to wipe the forearms clean by using dry facial tissue. The inner sides of the forearms of both hands of the testee are marked with measuring areas, the area of the testing area is 4cm multiplied by 4cm, the same arm can simultaneously mark a plurality of areas, and each testing area is at least 1cm apart. Before formal test, people should sit still in a room meeting the standard for more than 20min without drinking water and beverages. The forearms were exposed and left in the test condition to remain relaxed. The test sample was evenly spread over the test area using a latex finger cuff at a rate of 2.0mg/cm 2. The skin moisture content was measured before and 30, 60, 90, 120, 150, 180, 210, 240min after sample application, and the skin moisture change rate was calculated. The skin moisture content testing operation is completed by the same person, and the skin moisture change rate calculation formula is as follows:

rate of change of skin moisture ═ m_n－m₀)×100％/m₀。

In the formula: m is_nRepresents the measurement value at the n hour after the sample is coated; m is₀The measured values before coating the sample are shown. And (3) performing data analysis by using SPSS 12.0 statistical software, and judging that the difference is significant if the change rate of the skin moisture is less than 0.05.

As can be seen from FIG. 3, at all the different test time points, the skin moisture change rate caused by the application of the moisturizing skin cream containing the asparagus unsaturated fatty acid is significantly higher than that caused by the application of the blank control common skin cream (P is less than 0.05), thereby indicating that the asparagus unsaturated fatty acid has better moisturizing performance.

It should be noted that the experimental procedures for verifying the moisturizing abilities of the moisturizing skin care oil containing unsaturated fatty acids of asparagus in example 2 and the moisturizing skin care milk containing unsaturated fatty acids of asparagus in example 3 are similar to those in examples 4 and 5, and the obtained experimental results can also reach corresponding expectations (compared with the ordinary skin care oil or the ordinary skin care milk, the moisturizing skin care oil or the moisturizing skin care milk containing unsaturated fatty acids of asparagus has a better moisturizing effect), and are not repeated herein.

It should be further noted that other contents of the asparagus unsaturated fatty acid moisturizing skin care product and the preparation method thereof disclosed by the invention can be found in the prior art, and are not described herein again.

In addition, it should be noted that the technical solutions in the embodiments may be combined with each other, but must be based on the realization of the technical solutions by those skilled in the art, and when the technical solutions are contradictory or cannot be realized, such a combination of the technical solutions should not be considered to exist, and is not within the protection scope of the present invention.

The above description is only an alternative embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications and equivalents of the present invention, which are made by the contents of the present specification and the accompanying drawings, or directly/indirectly applied to other related technical fields, are included in the scope of the present invention.