阅读说明：本技术 一种具有降血脂功能的组合物及其应用 (Composition with blood fat reducing function and application thereof ) 是由 于洪林 于 2021-10-11 设计创作，主要内容包括：本发明涉及一种具有降血脂功能的组合物及其应用,属于生物技术领域。本发明提供了一种具有降血脂功能的组合物,所述组合物的原料包括黑木耳1500～2100份、山楂15～25份、沙棘90～150份、人参8～12份、马齿苋30～50份以及紫苏子8～12份；所述组合物可显著降低高脂血症患者的甘油三酯、总胆固醇等高血脂指标,并且,所述组合物使用的原料均为药食同源,因此,使用所述组合物降血脂具有效果好以及无副作用的优势。(The invention relates to a composition with a blood fat reducing function and application thereof, belonging to the technical field of biology. The invention provides a composition with a blood fat reducing function, which comprises 1500-2100 parts of black fungus, 15-25 parts of hawthorn, 90-150 parts of sea buckthorn, 8-12 parts of ginseng, 30-50 parts of purslane and 8-12 parts of perilla seed; the composition can obviously reduce the high blood lipid indexes such as triglyceride, total cholesterol and the like of a patient with hyperlipidemia, and raw materials used by the composition are all homologous in medicine and food, so the composition has the advantages of good effect and no side effect.)

1. The composition with the function of reducing blood fat is characterized by comprising the following raw materials in parts by weight:

1500-2100 parts of black fungus, 15-25 parts of hawthorn, 90-150 parts of sea buckthorn, 8-12 parts of ginseng, 30-50 parts of purslane and 8-12 parts of perilla seed.

2. The composition of claim 1, wherein the composition is prepared from the following raw materials in parts by weight:

1800 parts of black fungus, 20 parts of hawthorn, 120 parts of sea buckthorn, 10 parts of ginseng, 40 parts of purslane and 10 parts of perilla seed.

3. A process for the preparation of a composition according to claim 1 or 2, characterized in that it comprises the following steps:

weighing raw materials: weighing black fungus, hawthorn, sea buckthorn, ginseng, purslane and perilla fruit in different parts by weight according to the raw material formula of the composition of claim 1 or 2;

black fungus treatment: crushing black fungus to obtain black fungus powder; mixing the black fungus powder with water, and performing ultrasonic extraction at 60-80 ℃ for 0.5-1 h to obtain an extracting solution; carrying out solid-liquid separation on the extracting solution, and taking filtrate; drying the filtrate to prepare powder to obtain black fungus essence;

the hawthorn processing step comprises: pulverizing fructus crataegi to obtain fructus crataegi powder; adding hawthorn powder into an organic solvent, and leaching for 3-4 hours to obtain a leaching solution; carrying out solid-liquid separation on the leaching solution, and taking filtrate; drying the filtrate to obtain fructus crataegi essence;

the sea buckthorn fruit processing steps are as follows: juicing fructus Hippophae, and performing solid-liquid separation to obtain fructus Hippophae juice; deoiling fructus Hippophae juice to obtain deoiled fructus Hippophae juice; concentrating deoiled fructus Hippophae juice, drying, and making into powder to obtain fructus Hippophae essence;

the ginseng processing step comprises: pulverizing Ginseng radix to obtain Ginseng radix powder; adding ginseng powder into an organic solvent, and leaching for 6-8 hours to obtain a leaching solution; carrying out solid-liquid separation on the leaching solution, and taking filtrate; drying the filtrate to obtain Ginseng radix essence;

purslane processing steps: pulverizing herba Portulacae to obtain herba Portulacae powder; adding purslane powder into an organic solvent, and leaching for 2-3 hours to obtain a leaching solution; carrying out solid-liquid separation on the leaching solution, and taking filtrate; drying the filtrate to obtain herba Portulacae essence;

the processing step of the perilla seeds: pulverizing fructus Perillae to obtain fructus Perillae powder; adding perilla seed powder into an organic solvent, and leaching for 3-4 hours to obtain a leaching solution; carrying out solid-liquid separation on the leaching solution, and taking filtrate; drying the filtrate to obtain fructus Perillae essence;

mixing: mixing Auricularia auricula essence, fructus crataegi essence, fructus Hippophae essence, Ginseng radix essence, herba Portulacae essence and fructus Perillae essence to obtain the composition of claim 1 or 2.

4. The preparation method according to claim 3, wherein the mixing mass ratio of the black fungus powder to water is 1-5: 60 to 150.

5. The preparation method according to claim 3 or 4, wherein the mixing mass ratio of the hawthorn powder to the organic solvent is 1-5: 40 to 100.

6. The method according to any one of claims 3 to 5, wherein the mixing mass ratio of the ginseng powder to the organic solvent is 1 to 5: 60 to 100.

7. The preparation method of any one of claims 3 to 6, wherein the purslane powder and the organic solvent are mixed at a mass ratio of 1-10: 40 to 50.

8. The method according to any one of claims 3 to 7, wherein the mixing mass ratio of the perilla seed powder to the organic solvent is 1 to 5: 50 to 100.

9. Use of a composition according to any one of claims 1 to 8 in the preparation of a food product.

10. Use of the composition of any one of claims 1 to 4 as an active ingredient in the preparation of a medicament having a hypolipidemic effect.

Technical Field

The invention relates to a composition with a blood fat reducing function and application thereof, belonging to the technical field of biology.

Background

Hyperlipidemia refers to the condition of high blood lipid level, which can directly cause some diseases seriously harming human health, such as atherosclerosis, coronary heart disease, pancreatitis, etc.

Hyperlipidemia can be classified into primary and secondary. Primary is associated with both congenital and genetic origin, either due to monogenic or polygenic deficiencies, abnormalities in receptors, enzymes or apolipoproteins involved in lipoprotein transport and metabolism, or due to environmental factors (diet, nutrition, drugs) and by unknown mechanisms. The secondary symptoms are caused by metabolic disorder diseases (diabetes, hypertension, myxoedema, hypothyroidism, obesity, liver and kidney diseases, and adrenal cortex hyperfunction), or related to other factors such as age, sex, season, drinking, smoking, diet, physical activity, mental stress, emotional activity, etc.

Dyslipidemia is often accompanied by a variety of cardiovascular risk factors. According to related data, the first onset age 1/3 of patients with cardiovascular and cerebrovascular diseases in China is below 60 years old, the onset rate is up to 13.6%, and the onset rate of cardiovascular and cerebrovascular diseases of old people above 65 years old is up to 95%. Hyperlipidemia is also an important risk factor for the promotion of hypertension, impaired glucose tolerance, and diabetes. Hyperlipidemia can also lead to fatty liver, liver cirrhosis, cholelithiasis, pancreatitis, fundus hemorrhage, blindness, peripheral vascular disease, claudication, and hyperuricemia. Some patients with primary and familial hyperlipidemia may also develop tendinous, nodular, palmar and periorbital xanthoma, youth corneal arcus, etc. The data report that the number of people suffering from dyslipidemia of adults in China reaches 1.8 hundred million. The incidence and death rate of cardiovascular and cerebrovascular diseases caused by the disease are high, and the death rate of cardiovascular and cerebrovascular diseases accounts for 40.72 percent of the total death rate. The disability rate of patients with cerebral apoplexy is as high as 75 percent, and patients with coronary heart disease become half-labor-force countless. Cardiovascular and cerebrovascular diseases, myocardial infarction and cerebral infarction, and the recurrence rate within five years is as high as 42%. Experts have also promulgated that in recent years the prevalence of blood lipids in adults in our country is 18.6%, and the potential hazards and consequences must alert us.

In general, secondary hyperlipidemia can be effectively controlled through lifestyle intervention such as physical exercise and diet adjustment. However, the harm of most people to hyperlipidemia is not recognized and regarded as important enough, so the effect of life style intervention treatment on hyperlipidemia is not good. At present, the treatment of hyperlipidemia mainly depends on drug treatment.

Statins such as lovastatin, simvastatin and atorvastatin and fibrates such as bezafibrate and fenofibrate have the function of reducing blood fat. However, the statin drugs, when combined with other hypolipidemic drugs, increase the adverse effects of the drugs, such as symptoms of abdominal pain, constipation, muscle soreness, insomnia, and the like. The fibrate drugs have obvious adverse reactions, the main adverse reactions of the fibrate drugs are gastrointestinal reactions, and patients with liver and kidney functions should not take the fibrate drugs.

Besides western medicine treatment, some Chinese patent medicines with the efficacy of reducing blood fat exist in China, but the Chinese patent medicines have obvious side effects. For example, "Xuezhikang" clearly suggests that the common adverse reactions of this herb are gastrointestinal discomfort, such as stomach ache, abdominal distension, burning stomach, etc. Occasionally, the reversibility of serum aminotransferase and creatine phosphokinase can be increased. Rarely symptoms of weakness, dry mouth, dizziness, headache, myalgia, rash, gall bladder pain, edema, conjunctival congestion and urinary tract irritation ".

Therefore, there is a need to find a drug with a hypolipidemic effect with little or no side effects.

Disclosure of Invention

In order to solve the problems, the invention provides a composition with a function of reducing blood fat, which comprises the following raw materials in parts by weight:

1500-2100 parts of black fungus, 15-25 parts of hawthorn, 90-150 parts of sea buckthorn, 8-12 parts of ginseng, 30-50 parts of purslane and 8-12 parts of perilla seed.

In one embodiment of the present invention, the composition is prepared from the following raw materials in parts by weight:

1800 parts of black fungus, 20 parts of hawthorn, 120 parts of sea buckthorn, 10 parts of ginseng, 40 parts of purslane and 10 parts of perilla seed.

The invention also provides a preparation method of the composition, which comprises the following steps:

weighing raw materials: weighing black fungus, hawthorn, sea buckthorn, ginseng, purslane and perilla seed in different parts by weight according to the raw material formula of the composition;

black fungus treatment: crushing black fungus to obtain black fungus powder; mixing the black fungus powder with water, and performing ultrasonic extraction at 60-80 ℃ for 0.5-1 h to obtain an extracting solution; carrying out solid-liquid separation on the extracting solution, and taking filtrate; drying the filtrate to prepare powder to obtain black fungus essence;

the hawthorn processing step comprises: pulverizing fructus crataegi to obtain fructus crataegi powder; adding hawthorn powder into an organic solvent, and leaching for 3-4 hours to obtain a leaching solution; carrying out solid-liquid separation on the leaching solution, and taking filtrate; drying the filtrate to obtain fructus crataegi essence;

the sea buckthorn fruit processing steps are as follows: juicing fructus Hippophae, and performing solid-liquid separation to obtain fructus Hippophae juice; deoiling fructus Hippophae juice to obtain deoiled fructus Hippophae juice; concentrating deoiled fructus Hippophae juice, drying, and making into powder to obtain fructus Hippophae essence;

the ginseng processing step comprises: pulverizing Ginseng radix to obtain Ginseng radix powder; adding ginseng powder into an organic solvent, and leaching for 6-8 hours to obtain a leaching solution; carrying out solid-liquid separation on the leaching solution, and taking filtrate; drying the filtrate to obtain Ginseng radix essence;

purslane processing steps: pulverizing herba Portulacae to obtain herba Portulacae powder; adding purslane powder into an organic solvent, and leaching for 2-3 hours to obtain a leaching solution; carrying out solid-liquid separation on the leaching solution, and taking filtrate; drying the filtrate to obtain herba Portulacae essence;

the processing step of the perilla seeds: pulverizing fructus Perillae to obtain fructus Perillae powder; adding perilla seed powder into an organic solvent, and leaching for 3-4 hours to obtain a leaching solution; carrying out solid-liquid separation on the leaching solution, and taking filtrate; drying the filtrate to obtain fructus Perillae essence;

mixing: mixing Auricularia auricula essence, fructus crataegi essence, fructus Hippophae essence, Ginseng radix essence, herba Portulacae essence and fructus Perillae essence to obtain the composition.

In one embodiment of the invention, the mixing mass ratio of the black fungus powder to water is 1-5: 60 to 150.

In one embodiment of the invention, the mixing mass ratio of the hawthorn powder to the organic solvent is 1-5: 40 to 100.

In one embodiment of the present invention, the mixing mass ratio of the ginseng powder to the organic solvent is 1 to 5: 60 to 100.

In one embodiment of the invention, the mixing mass ratio of the purslane powder to the organic solvent is 1-10: 40 to 50.

In one embodiment of the invention, the mixing mass ratio of the perilla seed powder to the organic solvent is 1-5: 50 to 100.

In one embodiment of the present invention, the deoiling means: separating juice and fruit oil in the seabuckthorn juice by centrifugation, and then removing the fruit oil to obtain the deoiled seabuckthorn juice.

In one embodiment of the present invention, the organic solvent is an ethanol solution.

In one embodiment of the present invention, the ethanol solvent is 30-80% (v/v) ethanol solution.

In one embodiment of the present invention, the solid-liquid separation is filtration.

The invention also provides application of the composition in preparing food.

In one embodiment of the invention, the food product comprises at least one of a tabletted candy, a solid beverage, and a liquid beverage.

The invention also provides application of the composition as an active ingredient in preparation of a medicament with a blood fat reducing function.

The technical scheme of the invention has the following advantages:

the invention provides a composition with a blood fat reducing function, which comprises 1500-2100 parts of black fungus, 15-25 parts of hawthorn, 90-150 parts of sea buckthorn, 8-12 parts of ginseng, 30-50 parts of purslane and 8-12 parts of perilla seed; the composition can obviously reduce the high blood lipid indexes such as triglyceride, total cholesterol and the like of a patient with hyperlipidemia, and raw materials used by the composition are all homologous in medicine and food, so the composition has the advantages of good effect and no side effect.

Detailed Description

The following examples are provided to further understand the present invention, not to limit the scope of the present invention, but to provide the best mode, not to limit the content and the protection scope of the present invention, and any product similar or similar to the present invention, which is obtained by combining the present invention with other prior art features, falls within the protection scope of the present invention.

The following examples do not show specific experimental procedures or conditions, and can be performed according to the procedures or conditions of the conventional experimental procedures described in the literature in the field. The reagents or instruments used are not indicated by manufacturers, and are all conventional reagent products which can be obtained commercially.

Example 1: composition with blood fat reducing function and preparation method thereof

The embodiment provides a composition with a function of reducing blood fat, which is prepared from the following raw materials:

1800g of black fungus, 20g of hawthorn, 120g of sea buckthorn, 10g of ginseng, 40g of purslane and 10g of perilla fruit (the black fungus, the hawthorn, the sea buckthorn, the ginseng, the purslane and the perilla fruit are all purchased from Chinese medicinal material companies, wherein the ginseng is artificially planted for less than 5 years).

The preparation method of the composition comprises the following steps:

weighing raw materials: according to the raw material formula of the composition, 1800g of black fungus, 20g of hawthorn, 120g of sea buckthorn, 10g of ginseng, 40g of purslane and 10g of perilla seed are weighed;

black fungus treatment: crushing black fungus to 30 meshes by using a medicine crusher to obtain black fungus powder; mixing black fungus powder and water according to a mass ratio of 1: 40, and performing ultrasonic extraction at 80 ℃ for 0.5h to obtain an extracting solution; filtering the extractive solution to obtain filtrate; drying the filtrate to prepare powder to obtain black fungus essence;

the hawthorn processing step comprises: pulverizing fructus crataegi to 30 mesh with medicinal pulverizer to obtain fructus crataegi powder; according to the mass ratio of 1: 30 adding hawthorn powder into 50% (v/v) ethanol solution for leaching for 3 hours to obtain leaching solution; filtering the leaching solution to obtain filtrate; drying the filtrate to obtain fructus crataegi essence;

the sea buckthorn fruit processing steps are as follows: juicing fructus Hippophae with a screw squeezer to obtain fructus Hippophae juice; centrifuging fructus Hippophae juice at 3000r/min for 15min with high speed centrifuge until the juice and fruit oil in fructus Hippophae juice are separated, and removing the fruit oil to obtain deoiled fructus Hippophae juice; concentrating deoiled fructus Hippophae juice, drying, and making into powder to obtain fructus Hippophae essence;

the ginseng processing step comprises: pulverizing Ginseng radix to 30 mesh with medicinal pulverizer to obtain Ginseng radix powder; according to the mass ratio of 1: 30 adding ginseng powder into an ethanol solution with the concentration of 60% (v/v) and leaching for 6 hours to obtain a leaching solution; filtering the leaching solution to obtain filtrate; drying the filtrate to obtain Ginseng radix essence;

purslane processing steps: pulverizing herba Portulacae to 30 mesh with a medicine pulverizer to obtain herba Portulacae powder; according to the mass ratio of 1: 15 adding purslane powder into 50% (v/v) ethanol solution to be leached for 2 hours to obtain leaching solution; filtering the leaching solution to obtain filtrate; drying the filtrate to obtain herba Portulacae essence;

the processing step of the perilla seeds: pulverizing fructus Perillae into 30 mesh powder with medicinal pulverizer to obtain fructus Perillae powder; according to the mass ratio of 1: 30 adding perilla seed powder into 50% (v/v) ethanol solution for leaching for 3 hours to obtain leaching solution; filtering the leaching solution to obtain filtrate; drying the filtrate to obtain fructus Perillae essence;

mixing: mixing Auricularia auricula essence, fructus crataegi essence, fructus Hippophae essence, Ginseng radix essence, herba Portulacae essence and fructus Perillae essence to obtain the composition.

Example 2: composition with blood fat reducing function and preparation method thereof

The embodiment provides a composition with a function of reducing blood fat, which is prepared from the following raw materials:

2000g of black fungus, 15g of hawthorn, 90g of sea buckthorn, 8g of ginseng, 30g of purslane and 8g of perilla fruit (the black fungus, the hawthorn, the sea buckthorn, the ginseng, the purslane and the perilla fruit are all purchased from Chinese medicinal material companies, wherein the ginseng is artificially planted for less than 5 years).

The preparation method of the composition comprises the following steps:

weighing raw materials: weighing 2000g of black fungus, 15g of hawthorn, 90g of sea buckthorn, 8g of ginseng, 30g of purslane and 8g of perilla fruit according to the raw material formula of the composition;

black fungus treatment: crushing black fungus to 30 meshes by using a medicine crusher to obtain black fungus powder; mixing black fungus powder and water according to a mass ratio of 1: 50, mixing, and performing ultrasonic extraction at 70 ℃ for 1h to obtain an extracting solution; filtering the extractive solution to obtain filtrate; drying the filtrate to prepare powder to obtain black fungus essence;

the hawthorn processing step comprises: pulverizing fructus crataegi to 30 mesh with medicinal pulverizer to obtain fructus crataegi powder; according to the mass ratio of 1: 40 adding the hawthorn powder into an ethanol solution with the concentration of 40% (v/v) and leaching for 4 hours to obtain a leaching solution; filtering the leaching solution to obtain filtrate; drying the filtrate to obtain fructus crataegi essence;

the sea buckthorn fruit processing steps are as follows: juicing fructus Hippophae with a screw squeezer to obtain fructus Hippophae juice; centrifuging fructus Hippophae juice at 3000r/min for 15min with high speed centrifuge until the juice and fruit oil in fructus Hippophae juice are separated, and removing the fruit oil to obtain deoiled fructus Hippophae juice; concentrating deoiled fructus Hippophae juice, drying, and making into powder to obtain fructus Hippophae essence;

the ginseng processing step comprises: pulverizing Ginseng radix to 30 mesh with medicinal pulverizer to obtain Ginseng radix powder; according to the mass ratio of 1: 50 adding ginseng powder into an ethanol solution with the concentration of 70% (v/v) to be leached for 8 hours to obtain a leaching solution; filtering the leaching solution to obtain filtrate; drying the filtrate to obtain Ginseng radix essence;

purslane processing steps: pulverizing herba Portulacae to 30 mesh with a medicine pulverizer to obtain herba Portulacae powder; according to the mass ratio of 1: 30 adding purslane powder into an ethanol solution with the concentration of 40% (v/v) and leaching for 3 hours to obtain a leaching solution; filtering the leaching solution to obtain filtrate; drying the filtrate to obtain herba Portulacae essence;

the processing step of the perilla seeds: pulverizing fructus Perillae into 30 mesh powder with medicinal pulverizer to obtain fructus Perillae powder; according to the mass ratio of 1: 40 adding perilla seed powder into an ethanol solution with the concentration of 40% (v/v) and leaching for 4 hours to obtain a leaching solution; filtering the leaching solution to obtain filtrate; drying the filtrate to obtain fructus Perillae essence;

mixing: mixing Auricularia auricula essence, fructus crataegi essence, fructus Hippophae essence, Ginseng radix essence, herba Portulacae essence and fructus Perillae essence to obtain the composition.

Example 3: composition with blood fat reducing function and preparation method thereof

The embodiment provides a composition with a function of reducing blood fat, which is prepared from the following raw materials:

2100g of black fungus, 25g of hawthorn, 150g of sea buckthorn, 12g of ginseng, 50g of purslane and 12g of perilla seed (the black fungus, the hawthorn, the sea buckthorn, the ginseng, the purslane and the perilla seed are all purchased from traditional Chinese medicine companies, wherein the ginseng is artificially planted for less than 5 years).

The preparation method of the composition comprises the following steps:

weighing raw materials: according to the raw material formula of the composition, 2100g of black fungus, 25g of hawthorn, 150g of sea buckthorn, 12g of ginseng, 50g of purslane and 12g of perilla seed are weighed;

black fungus treatment: crushing black fungus to 30 meshes by using a medicine crusher to obtain black fungus powder; mixing black fungus powder and water according to a mass ratio of 1: 45, and performing ultrasonic extraction at 60 ℃ for 1h to obtain an extracting solution; filtering the extractive solution to obtain filtrate; drying the filtrate to prepare powder to obtain black fungus essence;

the hawthorn processing step comprises: pulverizing fructus crataegi to 30 mesh with medicinal pulverizer to obtain fructus crataegi powder; according to the mass ratio of 1: 30 adding hawthorn powder into an ethanol solution with the concentration of 45% (v/v) and leaching for 4 hours to obtain a leaching solution; filtering the leaching solution to obtain filtrate; drying the filtrate to obtain fructus crataegi essence;

the sea buckthorn fruit processing steps are as follows: juicing fructus Hippophae with a screw squeezer to obtain fructus Hippophae juice; centrifuging fructus Hippophae juice at 3000r/min for 15min with high speed centrifuge until the juice and fruit oil in fructus Hippophae juice are separated, and removing the fruit oil to obtain deoiled fructus Hippophae juice; concentrating deoiled fructus Hippophae juice, drying, and making into powder to obtain fructus Hippophae essence;

the ginseng processing step comprises: pulverizing Ginseng radix to 30 mesh with medicinal pulverizer to obtain Ginseng radix powder; according to the mass ratio of 1: 40 adding the ginseng powder into 65% (v/v) ethanol solution for leaching for 7 hours to obtain leaching solution; filtering the leaching solution to obtain filtrate; drying the filtrate to obtain Ginseng radix essence;

purslane processing steps: pulverizing herba Portulacae to 30 mesh with a medicine pulverizer to obtain herba Portulacae powder; according to the mass ratio of 1: 25 adding purslane powder into an ethanol solution with the concentration of 55% (v/v) and leaching for 3 hours to obtain a leaching solution; filtering the leaching solution to obtain filtrate; drying the filtrate to obtain herba Portulacae essence;

the processing step of the perilla seeds: pulverizing fructus Perillae into 30 mesh powder with medicinal pulverizer to obtain fructus Perillae powder; according to the mass ratio of 1: 35 adding the perilla seed powder into an ethanol solution with the concentration of 45% (v/v) and leaching for 4 hours to obtain a leaching solution; filtering the leaching solution to obtain filtrate; drying the filtrate to obtain fructus Perillae essence;

mixing: mixing Auricularia auricula essence, fructus crataegi essence, fructus Hippophae essence, Ginseng radix essence, herba Portulacae essence and fructus Perillae essence to obtain the composition.

Comparative example 1: composition with blood fat reducing function and preparation method thereof

The comparative example provides a composition with the function of reducing blood fat, and the composition is prepared from the following raw materials:

2100g of black fungus, 25g of hawthorn, 150g of sea buckthorn, 12g of onion, 50g of purslane and 12g of perilla seed (the black fungus, the hawthorn, the sea buckthorn, the onion, the purslane and the perilla seed are all purchased from Chinese medicinal material companies).

The preparation method of the composition comprises the following steps:

weighing raw materials: according to the raw material formula of the composition, 2100g of black fungus, 25g of hawthorn, 150g of sea buckthorn, 12g of onion, 50g of purslane and 12g of perilla seed are weighed;

black fungus treatment: crushing black fungus to 30 meshes by using a medicine crusher to obtain black fungus powder; mixing black fungus powder and water according to a mass ratio of 1: 45, and performing ultrasonic extraction at 60 ℃ for 1h to obtain an extracting solution; filtering the extractive solution to obtain filtrate; drying the filtrate to prepare powder to obtain black fungus essence;

the hawthorn processing step comprises: pulverizing fructus crataegi to 30 mesh with medicinal pulverizer to obtain fructus crataegi powder; according to the mass ratio of 1: 30 adding hawthorn powder into an ethanol solution with the concentration of 45% (v/v) and leaching for 4 hours to obtain a leaching solution; filtering the leaching solution to obtain filtrate; drying the filtrate to obtain fructus crataegi essence;

the sea buckthorn fruit processing steps are as follows: juicing fructus Hippophae with a screw squeezer to obtain fructus Hippophae juice; centrifuging fructus Hippophae juice at 3000r/min for 15min with high speed centrifuge until the juice and fruit oil in fructus Hippophae juice are separated, and removing the fruit oil to obtain deoiled fructus Hippophae juice; concentrating deoiled fructus Hippophae juice, drying, and making into powder to obtain fructus Hippophae essence;

onion treatment: crushing onion to 30 meshes by using a dehydrated onion crusher to obtain onion powder; according to the mass ratio of 1: 15 adding onion powder into 60% (v/v) ethanol solution, and soaking at 70 deg.C for 0.5 hr to obtain extractive solution; filtering the leaching solution to obtain filtrate; drying the filtrate to obtain Bulbus Allii Cepae essence;

purslane processing steps: pulverizing herba Portulacae to 30 mesh with a medicine pulverizer to obtain herba Portulacae powder; according to the mass ratio of 1: 25 adding purslane powder into an ethanol solution with the concentration of 55% (v/v) and leaching for 3 hours to obtain a leaching solution; filtering the leaching solution to obtain filtrate; drying the filtrate to obtain herba Portulacae essence;

the processing step of the perilla seeds: pulverizing fructus Perillae into 30 mesh powder with medicinal pulverizer to obtain fructus Perillae powder; according to the mass ratio of 1: 35 adding the perilla seed powder into an ethanol solution with the concentration of 45% (v/v) and leaching for 4 hours to obtain a leaching solution; filtering the leaching solution to obtain filtrate; drying the filtrate to obtain fructus Perillae essence;

mixing: mixing Auricularia auricula essence, fructus crataegi essence, fructus Hippophae essence, Bulbus Allii Cepae essence, herba Portulacae essence and fructus Perillae essence to obtain the composition.

Comparative example 2: composition with blood fat reducing function and preparation method thereof

The comparative example provides a composition with the function of reducing blood fat, and the composition is prepared from the following raw materials: 2000g of black fungus, 15g of hawthorn, 90g of momordica grosvenori, 8g of onion, 30g of purslane and 8g of perilla fruit (the black fungus, the hawthorn, the momordica grosvenori, the onion, the purslane and the perilla fruit are all purchased from Chinese medicinal material companies, wherein ginseng is artificially planted for less than 5 years).

The preparation method of the composition comprises the following steps:

weighing raw materials: weighing 2000g of black fungus, 15g of hawthorn, 90g of momordica grosvenori, 8g of onion, 30g of purslane and 8g of perilla fruit according to the raw material formula of the composition;

black fungus treatment: crushing black fungus to 30 meshes by using a medicine crusher to obtain black fungus powder; mixing black fungus powder and water according to a mass ratio of 1: 50, mixing, and performing ultrasonic extraction at 70 ℃ for 1h to obtain an extracting solution; filtering the extractive solution to obtain filtrate; drying the filtrate to prepare powder to obtain black fungus essence;

the hawthorn processing step comprises: pulverizing fructus crataegi to 30 mesh with medicinal pulverizer to obtain fructus crataegi powder; according to the mass ratio of 1: 40 adding the hawthorn powder into an ethanol solution with the concentration of 40% (v/v) and leaching for 4 hours to obtain a leaching solution; filtering the leaching solution to obtain filtrate; drying the filtrate to obtain fructus crataegi essence;

the method comprises the following steps: crushing the momordica grosvenori to 30 meshes by using a medicine crusher to obtain momordica grosvenori powder; according to the mass ratio of 1: 30 adding the fructus momordicae powder into water, and leaching for 3 hours at 90 ℃ to obtain a leaching solution; filtering the leaching solution to obtain filtrate; drying the filtrate to prepare powder to obtain fructus momordicae essence;

onion treatment: crushing onion to 30 meshes by using a dehydrated onion crusher to obtain onion powder; according to the mass ratio of 1: 15 adding onion powder into 60% (v/v) ethanol solution, and soaking at 70 deg.C for 0.5 hr to obtain extractive solution; filtering the leaching solution to obtain filtrate; drying the filtrate to obtain Bulbus Allii Cepae essence;

purslane processing steps: pulverizing herba Portulacae to 30 mesh with a medicine pulverizer to obtain herba Portulacae powder; according to the mass ratio of 1: 30 adding purslane powder into an ethanol solution with the concentration of 40% (v/v) and leaching for 3 hours to obtain a leaching solution; filtering the leaching solution to obtain filtrate; drying the filtrate to obtain herba Portulacae essence;

the processing step of the perilla seeds: pulverizing fructus Perillae into 30 mesh powder with medicinal pulverizer to obtain fructus Perillae powder; according to the mass ratio of 1: 40 adding perilla seed powder into 50% (v/v) ethanol solution for leaching for 4h to obtain leaching solution; filtering the leaching solution to obtain filtrate; drying the filtrate to obtain fructus Perillae essence;

mixing: mixing Auricularia auricula essence, fructus crataegi essence, fructus Siraitiae Grosvenorii essence, Bulbus Allii Cepae essence, herba Portulacae essence and fructus Perillae essence to obtain the composition.

Comparative example 3: composition with blood fat reducing function and preparation method thereof

The comparative example provides a composition with the function of reducing blood fat, and the composition is prepared from the following raw materials: 1800g of black fungus, 20g of hawthorn, 120g of momordica grosvenori, 10g of ginseng and 10g of perilla fruit (the black fungus, the hawthorn, the momordica grosvenori, the ginseng and the perilla fruit are all purchased from Chinese medicinal material companies, wherein the ginseng is artificially planted for less than 5 years).

The preparation method of the composition comprises the following steps:

weighing raw materials: according to the raw material formula of the composition, 1800g of black fungus, 20g of hawthorn, 120g of momordica grosvenori, 10g of ginseng and 10g of perilla seed are weighed;

black fungus treatment: crushing black fungus to 30 meshes by using a medicine crusher to obtain black fungus powder; mixing black fungus powder and water according to a mass ratio of 1: 40, and performing ultrasonic extraction at 80 ℃ for 0.5h to obtain an extracting solution; filtering the extractive solution to obtain filtrate; drying the filtrate to prepare powder to obtain black fungus essence;

the hawthorn processing step comprises: pulverizing fructus crataegi to 30 mesh with medicinal pulverizer to obtain fructus crataegi powder; according to the mass ratio of 1: 30 adding hawthorn powder into 50% (v/v) ethanol solution for leaching for 3 hours to obtain leaching solution; filtering the leaching solution to obtain filtrate; drying the filtrate to obtain fructus crataegi essence;

the method comprises the following steps: crushing the momordica grosvenori to 30 meshes by using a medicine crusher to obtain momordica grosvenori powder; according to the mass ratio of 1: 30 adding the fructus momordicae powder into water, and leaching for 3 hours at 90 ℃ to obtain a leaching solution; filtering the leaching solution to obtain filtrate; drying the filtrate to prepare powder to obtain fructus momordicae essence;

the ginseng processing step comprises: pulverizing Ginseng radix to 30 mesh with medicinal pulverizer to obtain Ginseng radix powder; according to the mass ratio of 1: 30 adding ginseng powder into an ethanol solution with the concentration of 60% (v/v) and leaching for 6 hours to obtain a leaching solution; filtering the leaching solution to obtain filtrate; drying the filtrate to obtain Ginseng radix essence;

the processing step of the perilla seeds: pulverizing fructus Perillae into 30 mesh powder with medicinal pulverizer to obtain fructus Perillae powder; according to the mass ratio of 1: 30 adding perilla seed powder into 50% (v/v) ethanol solution for leaching for 3 hours to obtain leaching solution; filtering the leaching solution to obtain filtrate; drying the filtrate to obtain fructus Perillae essence;

mixing: mixing Auricularia auricula essence, fructus crataegi essence, fructus Siraitiae Grosvenorii essence, Ginseng radix essence and fructus Perillae essence to obtain the composition.

Comparative example 4: composition with blood fat reducing function and preparation method thereof

The comparative example provides a composition with the function of reducing blood fat, and the composition is prepared from the following raw materials:

1800g of black fungus, 20g of hawthorn, 20g of onion, 2000g of purslane and 10g of perilla seed (the black fungus, the hawthorn, the onion, the purslane and the perilla seed are all purchased from Chinese medicinal material companies).

The preparation method of the composition comprises the following steps:

weighing raw materials: according to the raw material formula of the composition, 1800g of black fungus, 20g of hawthorn, 20g of onion, 2000g of purslane and 10g of perilla seed are weighed;

black fungus treatment: crushing black fungus to 30 meshes by using a medicine crusher to obtain black fungus powder; mixing black fungus powder and water according to a mass ratio of 1: 40, and performing ultrasonic extraction at 80 ℃ for 0.5h to obtain an extracting solution; filtering the extractive solution to obtain filtrate; drying the filtrate to prepare powder to obtain black fungus essence;

the hawthorn processing step comprises: pulverizing fructus crataegi to 30 mesh with medicinal pulverizer to obtain fructus crataegi powder; according to the mass ratio of 1: 30 adding hawthorn powder into 50% (v/v) ethanol solution for leaching for 3 hours to obtain leaching solution; filtering the leaching solution to obtain filtrate; drying the filtrate to obtain fructus crataegi essence;

onion treatment: crushing onion to 30 meshes by using a dehydrated onion crusher to obtain onion powder; according to the mass ratio of 1: 15 adding onion powder into 60% (v/v) ethanol solution, and soaking at 70 deg.C for 0.5 hr to obtain extractive solution; filtering the leaching solution to obtain filtrate; drying the filtrate to obtain Bulbus Allii Cepae essence;

purslane processing steps: pulverizing herba Portulacae to 30 mesh with a medicine pulverizer to obtain herba Portulacae powder; according to the mass ratio of 1: 15 adding purslane powder into 50% (v/v) ethanol solution to be leached for 2 hours to obtain leaching solution; filtering the leaching solution to obtain filtrate; drying the filtrate to obtain herba Portulacae essence;

the processing step of the perilla seeds: pulverizing fructus Perillae into 30 mesh powder with medicinal pulverizer to obtain fructus Perillae powder; according to the mass ratio of 1: 30 adding perilla seed powder into 50% (v/v) ethanol solution for leaching for 3 hours to obtain leaching solution; filtering the leaching solution to obtain filtrate; drying the filtrate to obtain fructus Perillae essence;

mixing: mixing Auricularia auricula essence, fructus crataegi essence, Bulbus Allii Cepae essence, herba Portulacae essence and fructus Perillae essence to obtain the composition.

Comparative example 5: composition with blood fat reducing function and preparation method thereof

The comparative example provides a composition with the function of reducing blood fat, and the composition is prepared from the following raw materials:

2100g of black fungus, 600g of hawthorn, 1000g of onion, 50g of purslane and 12g of perilla seed (the black fungus, the hawthorn, the onion, the purslane and the perilla seed are all purchased from Chinese medicinal material companies).

The preparation method of the composition comprises the following steps:

weighing raw materials: according to the raw material formula of the composition, 2100g of black fungus, 600g of hawthorn, 1000g of onion, 50g of purslane and 12g of perilla seed are weighed;

black fungus treatment: crushing black fungus to 30 meshes by using a medicine crusher to obtain black fungus powder; mixing black fungus powder and water according to a mass ratio of 1: 45, and performing ultrasonic extraction at 60 ℃ for 1h to obtain an extracting solution; filtering the extractive solution to obtain filtrate; drying the filtrate to prepare powder to obtain black fungus essence;

the hawthorn processing step comprises: pulverizing fructus crataegi to 30 mesh with medicinal pulverizer to obtain fructus crataegi powder; according to the mass ratio of 1: 30 adding hawthorn powder into an ethanol solution with the concentration of 45% (v/v) and leaching for 4 hours to obtain a leaching solution; filtering the leaching solution to obtain filtrate; drying the filtrate to obtain fructus crataegi essence;

onion treatment: crushing onion to 30 meshes by using a dehydrated onion crusher to obtain onion powder; according to the mass ratio of 1: 15 adding onion powder into 60% (v/v) ethanol solution, and soaking at 70 deg.C for 0.5 hr to obtain extractive solution; filtering the leaching solution to obtain filtrate; drying the filtrate to obtain Bulbus Allii Cepae essence;

purslane processing steps: pulverizing herba Portulacae to 30 mesh with a medicine pulverizer to obtain herba Portulacae powder; according to the mass ratio of 1: 25 adding purslane powder into an ethanol solution with the concentration of 55% (v/v) and leaching for 3 hours to obtain a leaching solution; filtering the leaching solution to obtain filtrate; drying the filtrate to obtain herba Portulacae essence;

the processing step of the perilla seeds: pulverizing fructus Perillae into 30 mesh powder with medicinal pulverizer to obtain fructus Perillae powder; according to the mass ratio of 1: 35 adding the perilla seed powder into an ethanol solution with the concentration of 45% (v/v) and leaching for 4 hours to obtain a leaching solution; filtering the leaching solution to obtain filtrate; drying the filtrate to obtain fructus Perillae essence;

mixing: mixing Auricularia auricula essence, fructus crataegi essence, Bulbus Allii Cepae essence, herba Portulacae essence and fructus Perillae essence to obtain the composition.

Experimental example 1: effect of different compositions on hyperlipidemic mice

The method comprises the following specific steps:

(1) grouping animals

Healthy male C57BL/6J mice with the age of 3 weeks and the weight of 18-22 g are randomly and cage-divided to be pre-fed for one week, and then the formal experiment is started, the mice are randomly divided into blank groups (feeding normal diet, 20 mice), building groups (feeding high-fat diet, 20 mice), experimental groups 1 (feeding high-fat diet + composition of the gavage example 1, 20 mice), experimental groups 2 (feeding high-fat diet + composition of the gavage example 2, 20 mice), experimental groups 3 (feeding high-fat diet + composition of the gavage example 3, 20 mice), control groups 1 (feeding high-fat diet + composition of the gavage comparative example 1, 20 mice), control groups 2 (feeding high-fat diet + composition of the gavage comparative example 2, 20 mice), control groups 3 (feeding high-fat diet + composition of the gavage comparative example 3, 20 mice), control groups 4 (feeding high-fat diet + composition of the gavage comparative example 4), 20) and control group 5 (fed high fat diet + gavage comparative example 5 composition, 20); the formula of normal and high fat diets are shown in table 1.

(2) Animal experiments

The breeding temperature of the mice is 24 +/-3 ℃, the humidity is 60 +/-10%, the animal rooms are lighted for 12h every day, and the animals are dark for 12 h; weighing every week, and recording the weight, feed intake and water intake of the mice every week; the gavage experiment was started 7 weeks after the official rearing, the mice of the experimental groups 1 to 3 and the control groups 1 to 5 were gavage with the compositions of examples 1 to 3 and comparative examples 1 to 5 (0.05g of the composition was dissolved in 0.5mL of water and then gavage was performed), respectively, at a gavage time of 16:00 in the afternoon and for a gavage time of 2 weeks, in a dose of 0.05 g/day.

On the last day of the experiment, the contents of Triglyceride (TG) and Total Cholesterol (TC) in the serum of each group of mice are measured after each group of mice is sacrificed;

the contents of Triglyceride (TG) and Total Cholesterol (TC) in serum were measured by a full-automatic biochemical analyzer, and the results are shown in Table 2.

As can be seen from Table 2, after the molding, the content of Triglyceride (TG) and Total Cholesterol (TC) in the mouse serum is significantly increased, and after the composition of the gavage examples 1-3, the content of Triglyceride (TG) and Total Cholesterol (TC) in the mouse serum is significantly reduced, and the reduction range is much higher than that of the composition of the gavage comparative examples 1-5.

TABLE 1 animal feed formulation

Note: (a) mixed mineral formula (1 kg): FeSO₄·H₂O(256g)，GuSO₄·H₂O(27.9g)，MnSO₄·H₂O(243.8g)，ZnSO₄·H₂O(137g)，Na₂SeO₃(0.5g), KI (0.7g), and the balance water.

TABLE 2 serum triglyceride and Total Cholesterol levels (units: mmol/L) in different groups of mice

Group of	Triglyceride content	Total cholesterol content	Group of	Triglyceride content	Total cholesterol content
						Blank group	0.82±0.28	2.43±0.35	Control group 1	1.17±0.35	4.74±0.79
Molding set	1.93±0.56	11.91±2.21	Control group 2	1.38±0.49	6.15±1.12
						Experimental group 1	0.92±0.24	3.56±0.68	Control group 3	1.34±0.51	6.36±0.98
Experimental group 2	0.97±0.29	2.92±0.57	Control group 4	1.29±0.26	7.12±1.23
						Experimental group 3	0.89±0.27	3.69±0.44	Control group 5	1.41±0.47	5.86±0.87

Experimental example 2: effect of the composition on patients with hyperlipidemia

The method comprises the following specific steps:

(1) clinical data

50 patients with hyperlipidemia are treated, wherein 28 men and 22 women are treated. The biggest 76 years old and the smallest 42 years old. Among the above-mentioned people, the shortest time to hyperlipidemia is more than one year, and the longest time is more than ten years (see table 3).

(2) Basis of treatment

The searched hyperlipidemia patients show that triglyceride or total cholesterol is higher than a normal value through a blood lipid test report, two indexes of part of the patients are higher than the normal value, and high-density lipoprotein or low-density lipoprotein of part of the patients exceeds the normal value. The concrete conditions are as follows: 21 patients with 1 index of triglyceride higher than normal value, 19 patients with 1 index of total cholesterol higher than normal value, 4 patients with 2 indexes of triglyceride and total cholesterol higher than normal value, and 1 patient with high triglyceride and low total cholesterol. Wherein 8 people have the experience that the blood fat is higher than the normal value after the medicine is orally taken and stopped for a period of time, 1 person (female) is chylemia, and the triglyceride is more than 7mmol/L (see table 3 specifically).

(III) methods of treatment

The composition of example 1 is orally administered to a patient once in the morning and evening, 1.6g with warm water each time, 3.2g daily, for 3 courses of treatment, 15 days each.

(IV) notes

Patients are advised to control the consumption of high fat, high sugar foods. No food or tea is compulsorily prohibited, and no change in life habits is required.

(V) method for evaluating therapeutic effects

Improvement: through more than two medical institutions blood drawing detection according to requirements, triglyceride and total cholesterol indexes of hyperlipidemic patients are obviously reduced and are close to normal values.

And (3) healing: through more than two medical institutions blood drawing detection according to requirements, triglyceride and total cholesterol indexes of a hyperlipemia patient are obviously reduced and reach normal values, and low-density lipoprotein and high-density lipoprotein approach or reach normal values;

wherein, the normal values of the triglyceride index are as follows: 0.25-1.8 mmol/L;

normal values for total cholesterol index are: 1.3-5.7 mmol/L;

normal values for the low density lipoprotein index are: 2.07-3.12 mmol/L;

normal values for the high density lipoprotein index are: 1.29-1.55 mmol/L;

the detection methods of triglyceride, total cholesterol, low density lipoprotein, and high density lipoprotein indexes are described in the literature, "national clinical laboratory practice (third edition)".

(VI) curative Effect results and analysis

In 50 patients, 41 patients were cured, 6 patients were improved, the curative effect was unstable in 3 patients, and the total effective rate was 97% (the treatment condition can be shown in table 4).

TABLE 350 basic conditions of the patients

TABLE 450 patients before and after treatment had a change in the content of triglyceride, total cholesterol, low density lipoprotein and high density lipoprotein (unit: mmol/L)

Note: "- -" is normal before treatment.

It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.