阅读说明：本技术 一种全自动pcr分析系统试剂盒安装和控制装置 (Full-automatic PCR analytic system kit installation and controlling means ) 是由 廖政 唐勇 潘颖 于 2021-08-30 设计创作，主要内容包括：本发明属于体外诊断技术领域,具体涉及一种全自动PCR分析系统试剂盒安装和控制装置。本发明包括骨架,骨架上安装有控制电路板,骨架上还分别安装有均与控制电路板电连接的变温扩增模块、旋转阀驱动模块、磁体升降模块和加热裂解模块,试剂盒上的微流控PCR芯片分别插入变温扩增模块、磁体升降模块和加热裂解模块,旋转阀驱动模块的输出端插入试剂盒内；所述旋转阀驱动模块包括与控制电路板电连接的旋转电机,旋转电机安装于骨架上,旋转电机的输出轴连接有用于驱动试剂盒上的旋转阀动作的驱动杆,驱动杆插入试剂盒的旋转阀内。本发明提供了一种结构简单的全自动PCR分析系统试剂盒安装和控制装置。(The invention belongs to the technical field of in-vitro diagnosis, and particularly relates to a kit installation and control device of a full-automatic PCR analysis system. The kit comprises a framework, wherein a control circuit board is arranged on the framework, a variable temperature amplification module, a rotary valve driving module, a magnet lifting module and a thermal cracking module which are electrically connected with the control circuit board are respectively arranged on the framework, a microfluidic PCR chip on the kit is respectively inserted into the variable temperature amplification module, the magnet lifting module and the thermal cracking module, and the output end of the rotary valve driving module is inserted into the kit; the rotary valve driving module comprises a rotary motor electrically connected with the control circuit board, the rotary motor is installed on the framework, an output shaft of the rotary motor is connected with a driving rod used for driving the rotary valve on the kit to act, and the driving rod is inserted into the rotary valve of the kit. The invention provides a device for installing and controlling a kit of a full-automatic PCR analysis system, which has a simple structure.)

1. The mounting and control device for the kit of the full-automatic PCR analysis system is characterized by comprising a framework (21), wherein a control circuit board (22) is mounted on the framework (21), a variable temperature amplification module (26), a rotary valve driving module (27), a magnet lifting module (28) and a heating and cracking module (29) which are electrically connected with the control circuit board (22) are respectively mounted on the framework (21), a microfluidic PCR chip (2103) on the kit (210) is respectively inserted into the variable temperature amplification module (26), the magnet lifting module (28) and the heating and cracking module (29), and the output end of the rotary valve driving module (27) is inserted into the kit (210); the rotary valve driving module (27) comprises a rotary motor (271) electrically connected with the control circuit board (22), the rotary motor (271) is installed on the framework (21), an output shaft of the rotary motor (271) is connected with a driving rod (272) used for driving a rotary valve on the reagent kit (210) to act, and the driving rod (272) is inserted into the rotary valve of the reagent kit (210).

2. The mounting and control device of the full-automatic PCR analysis system kit of claim 1, wherein the magnet lifting module (28) comprises a driving motor (281) mounted on the frame (21), the driving motor (281) is electrically connected with the control circuit board (22), a magnet (282) is connected to an output shaft of the driving motor (281), a magnet slot (283) is arranged on the magnet (282), and the microfluidic PCR chip (2103) passes through the magnet slot (283).

3. The mounting and control device for the reagent box of the full-automatic PCR analysis system according to claim 2, wherein a second touch sensor (284) for detecting whether the magnet (282) is lowered to the right position is further mounted on the mounting rack of the driving motor (281), and the second touch sensor (284) is electrically connected with the control circuit board (22).

4. The device for installing and controlling the kit of the full-automatic PCR analysis system according to claim 1, wherein a positioning frame (273) is further installed on the framework (21), a positioning plate (274) is installed on the driving rod (272), a valve position sensor (275) is sleeved on the positioning plate (274), and the valve position sensor (275) is installed on the positioning frame (273).

5. The device for mounting and controlling the reagent box of the full-automatic PCR analysis system according to claim 4, wherein the positioning plate (274) is provided with a plurality of positioning grooves.

6. The device for mounting and controlling the reagent cartridge of the full-automatic PCR analysis system according to claim 1, wherein the end of the driving rod (272) is perpendicular to the rotating shaft of the rotating motor (271), and the end of the driving rod (272) is engaged with the driving end of the rotating valve on the reagent cartridge (210).

7. The apparatus for mounting and controlling a reagent cartridge of a full-automatic PCR analysis system according to claim 1, wherein the number of the rotary motor (271) and the driving rods (272) is two, and the two driving rods (272) are respectively inserted into the rotary valve of the reagent cartridge (210) from two sides.

8. The mounting and control device for the reagent box of the full-automatic PCR analysis system according to claim 1, wherein a third touch sensor (276) is mounted on the inner side of the positioning frame (273), and the third touch sensor (276) is electrically connected with the control circuit board (22).

9. The device for installing and controlling the kit of the full-automatic PCR analysis system according to claim 1, wherein the temperature-variable amplification module (26) is provided with a slot for inserting the microfluidic PCR chip (2103).

10. The mounting and control device for the kit of the full-automatic PCR analysis system according to any one of claims 1 to 9, wherein the heating and cracking module (29) is provided with a slot for inserting the microfluidic PCR chip (2103).

Technical Field

The invention belongs to the technical field of in-vitro diagnosis, and particularly relates to a kit installation and control device of a full-automatic PCR analysis system.

Background

The technology of separating and purifying nucleic acid is a basic technology of biochemistry and molecular biology. With the wide application of molecular biology technology in biology, medicine and related fields, the nucleic acid separation and purification technology has been further developed. The development of molecular biology is greatly facilitated by the continuous emergence of various new methods, well-established classical methods and commercial reagent methods. Nucleic acids are biomacromolecules synthesized by the polymerization of many nucleotides, and are one of the most basic substances of life. Nucleic acid is widely present in all animal and plant cells and microorganisms, and nucleic acid in organisms is often combined with protein to form nucleoprotein. With the rapid development of molecular biology in recent years, nucleic acid-based molecular diagnostic and detection techniques have increasingly highlighted important roles in a variety of fields. The existing nucleic acid purification has the advantages of short time consumption, high purification precision and automatic treatment of a large batch of experimental samples; the fluorescence PCR analysis system is a method for monitoring the reaction process of PCR in real time by adding a fluorescent group on the basis of PCR and the change of a fluorescence signal and finally carrying out quantitative analysis on an unknown template. Currently, real-time fluorescence quantitative PCR has become an authoritative method for comparing quantitative differences in gene expression levels among different samples.

Over the past decade, this approach has rapidly become popular, involving multiple areas of science. However, the conventional PCR analysis system cannot automatically perform steps such as nucleic acid cleavage and extraction, and nucleic acid amplification by extracting a reagent to a desired position. In addition, the detection modules of the existing PCR analysis system need to be connected with the kit respectively, so that the structure is complex and the operation is difficult.

Disclosure of Invention

In order to solve the above problems of the prior art, the present invention provides a device for mounting and controlling a reagent cartridge of a fully automatic PCR analysis system, which has a simple structure.

The technical scheme adopted by the invention is as follows:

a full-automatic PCR analysis system kit installation and control device comprises a framework, wherein a control circuit board is installed on the framework, a variable temperature amplification module, a rotary valve driving module, a magnet lifting module and a pyrolysis module which are all electrically connected with the control circuit board are respectively installed on the framework, a microfluidic PCR chip on the kit is respectively inserted into the variable temperature amplification module, the magnet lifting module and the pyrolysis module, and the output end of the rotary valve driving module is inserted into a reagent box; the rotary valve driving module comprises a rotary motor electrically connected with the control circuit board, the rotary motor is installed on the framework, an output shaft of the rotary motor is connected with a driving rod used for driving the rotary valve on the kit to act, and the driving rod is inserted into the rotary valve of the kit.

When the kit is installed, the microfluidic PCR chip on the kit is sequentially inserted into the heating and cracking module, the magnet lifting module and the temperature-variable amplification module, so that the kit is reliably installed. The output end of the rotary valve driving module is inserted into the reagent box, so that the rotary valve can be accurately controlled. The magnetic lifting module and the heating cracking module are matched to complete the cracking and extracting functions of the nucleic acid, and finally the temperature-variable amplification module is used for completing the amplification of the nucleic acid. After the kit is installed in place, the control circuit board controls the variable temperature amplification module, the rotary valve driving module, the magnet lifting module and the pyrolysis module to act, so that automatic molecular diagnosis is realized.

As a preferred scheme of the invention, the magnet lifting module comprises a driving motor arranged on the framework, the driving motor is electrically connected with the control circuit board, an output shaft of the driving motor is connected with a magnet, a magnet slot is arranged on the magnet, and the microfluidic PCR chip passes through the magnet slot. When the drive motor drives the magnet to rise, the microfluidic PCR chip penetrates through the magnet slot, so that the separation of the magnetic beads in the kit is realized.

As a preferable scheme of the present invention, a second contact pressure sensor for detecting whether the magnet is lowered to the position is further installed on the mounting bracket of the driving motor, and the second contact pressure sensor is electrically connected to the control circuit board. When the magnet descends and extrudes the second touch sensor, the second touch sensor sends a signal to the control circuit board, and the control circuit board controls the driving motor to stop.

As a preferred scheme of the invention, the framework is also provided with a positioning frame, the driving rod is provided with a positioning disc, the positioning disc is sleeved with a valve position sensor, and the valve position sensor is arranged on the positioning frame. The opening position of the rotary valve is judged by the valve position sensor on the positioning frame through detecting the rotation angle of the positioning disc, so that automatic control is conveniently carried out. Therefore, the rotary valve is detected through the valve position sensor, the rotation condition of the rotary valve can be fed back, the rotary motor is driven according to the feedback condition, and the accuracy of the rotary valve in place rotation is further improved.

As a preferable scheme of the invention, a plurality of positioning grooves are arranged on the positioning disc. The valve position sensor judges the rotation angle of the positioning disk by detecting the positioning groove or the fan-shaped positioning sheet between the adjacent positioning grooves, so that the rotation angle of the rotary valve is judged.

In a preferred embodiment of the present invention, the end of the drive lever is perpendicular to the rotation shaft of the rotation motor, and the end of the drive lever is engaged with the drive end of the rotary valve on the reagent cartridge. The operation mouth of the rotary valve on the kit is provided with the draw-in groove, and when the actuating lever was inserted, the actuating lever just blocked in the draw-in groove of rotary valve operation mouth to when the rotating electrical machines started, the actuating lever can drive the rotary valve smoothly and rotate certain angle.

In a preferred embodiment of the present invention, the number of the rotary motors and the number of the driving rods are two, and the two driving rods are inserted into the rotary valves of the reagent cartridges from both sides, respectively. The two sides of the rotary valve are respectively rotated by the driving rods at the corresponding sides, so that the rotary valve can accurately rotate by a certain angle.

As a preferable scheme of the present invention, a third touch sensor is installed on the inner side of the positioning frame, and the third touch sensor is electrically connected to the control circuit board. When the reagent box is installed in place, the end part of the reagent box extrudes the third touch sensor, and the third touch sensor sends a signal to the control circuit board, so that whether the reagent box is installed in place can be accurately judged through the third touch sensor.

As a preferable scheme of the invention, the temperature-variable amplification module is provided with a slot for inserting the microfluidic PCR chip. When the kit is installed, the microfluidic PCR chip on the kit is inserted into the slot on the variable-temperature amplification module, so that the microfluidic PCR chip and the variable-temperature amplification module are realized, and the nucleic acid amplification of the reagent in the kit is automatically performed.

As a preferable scheme of the invention, the heating and cracking module is provided with a slot for inserting the microfluidic PCR chip. The microfluidic PCR chip on the kit is inserted into the slot of the heating and cracking module so as to crack nucleic acid.

The invention has the beneficial effects that:

1. the microfluidic PCR chip on the kit is respectively inserted into the temperature-variable amplification module, the magnet lifting module and the heating and cracking module, so that the kit is reliably installed. The output end of the rotary valve driving module is inserted into the reagent box, so that the rotary valve can be accurately controlled. The magnetic lifting module and the heating cracking module are matched to complete the cracking and extracting functions of the nucleic acid, and finally the temperature-variable amplification module is used for completing the amplification of the nucleic acid. After the kit is installed in place, the control circuit board controls the variable temperature amplification module, the rotary valve driving module, the magnet lifting module and the pyrolysis module to act, so that automatic molecular diagnosis is realized.

2. The rotary motor of the invention drives the driving rod to rotate, and then the driving rod drives the rotary valve to rotate to the corresponding position, thereby realizing the selection of different flow channels or reagents. The rotating angle of the driving rod can be accurately controlled by controlling the action of the rotating motor, so that the position of the rotating valve can be accurately adjusted, and the reagent can be ensured to flow to an accurate position.

Drawings

FIG. 1 is a schematic diagram of the structure of a fully automatic PCR analysis system;

FIG. 2 is a schematic diagram of the structure of an analysis module;

FIG. 3 is an exploded view of an analysis module;

figure 4 is a partial block diagram of an analysis module cartridge;

FIG. 5 is a front view of the piston lifting module and the membrane-piercing post module;

FIG. 6 is a perspective view of the piston lifting module and the membrane sealing piercing post module;

FIG. 7 is an assembly view of the present invention and a kit;

FIG. 8 is a schematic view of the structure of the kit;

FIG. 9 is a schematic structural view of the present invention;

fig. 10 is a schematic structural view of a magnet lifting module;

FIG. 11 is a schematic diagram of a rotary valve drive module;

FIG. 12 is a schematic structural diagram of a temperature-variable amplification module;

FIG. 13 is a partial block diagram of the skeleton;

FIG. 14 is a schematic structural diagram of an optical signal emission detection module and a temperature-variable amplification module;

FIG. 15 is a partial block diagram of an optical signal emission detection module;

FIG. 16 is a sectional view of a temperature-variable amplification module.

In the figure, 1 — analysis module; 2-analysis module movement; 3-a lifting door assembly; 4-a base; 5-a human-computer interaction module; 6-a fan; 7-an interface board; 8-a foot pad; 9-indicator light board; 10-an indicator light guide post; 21-a backbone; 22-a control circuit board; 23-optical signal emission detection module; 24-a piston lifting module; 25-sealing a membrane puncture column module; 26-a temperature-variable amplification module; 27-rotary valve drive module; 28-magnet lifting module; 29-a pyrolysis module; 210-a kit; 31-a lift gate motor; 32-a lift gate screw; 33-a lifting table; 34-a lifting door; 35-positioning a stop block; 211-a limiting groove; 231-a bidirectional motor; 232-a light source assembly; 233-emission light filter wheel; 234-receive optical filter disk; 235-light-receiving cone; 236-fiber optic condenser; 237-detection probe; 238-detecting the circuit board; 239-a rotational positioning sensor; 2310-optical signal housing; 241-a lifting motor; 242-lifting screw rod; 243-lifting plate; 244-a push rod; 245-a spacer; 246-a positioning sensor; 247-a guide cylinder; 248-guide column; 251-a rotating plate; 252-a lancing frame; 253-piercing rod; 254-a return spring; 255-a first touch pressure sensor; 261-a suspended heating block; 262-Peltier; 263-module housing; 264-a heat sink; 265-a radiator fan; 271-a rotary electric machine; 272-a drive rod; 273-a positioning frame; 274-positioning plate; 275-valve position sensor; 276-a third touch pressure sensor; 281-a drive motor; 282-a magnet; 283-magnet slots; 284-a second touch pressure sensor; 2101-piston bore; 2102-puncture hole; 2103-microfluidic PCR chip; 2104-rotary valve operating orifice; 2321-light source board; 2322 — excitation light source; 2461-squeeze piston position sensor; 2462-median position sensor; 2463-puncture position sensor.

Detailed Description

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the invention and are not to be construed as limiting the invention.

As shown in fig. 7 to 9 and 11, the device for installing and controlling a kit of a full-automatic PCR analysis system of the present embodiment includes a frame 21, a control circuit board 22 is installed on the frame 21, a temperature-variable amplification module 26, a rotary valve driving module 27, a magnet lifting module 28 and a thermal lysis module 29 which are electrically connected to the control circuit board 22 are respectively installed on the frame 21, a microfluidic PCR chip 2103 on the kit 210 is respectively inserted into the temperature-variable amplification module 26, the magnet lifting module 28 and the thermal lysis module 29, and an output end of the rotary valve driving module 27 is inserted into the kit 210; the rotary valve driving module 27 includes a rotary motor 271 electrically connected to the control circuit board 22, the rotary motor 271 is mounted on the frame 21, an output shaft of the rotary motor 271 is connected to a driving rod 272 for driving the rotary valve on the reagent kit 210 to operate, and the driving rod 272 is inserted into the rotary valve of the reagent kit 210.

It should be noted that: the end of the driving rod 272 is perpendicular to the rotation shaft of the rotating motor 271, and the end of the driving rod 272 is engaged with the driving end of the rotating valve of the reagent cartridge 210. The operation port of the rotary valve on the reagent kit 210 is provided with a slot, when the driving rod 272 is inserted, the driving rod 272 is just clamped in the slot of the operation port of the rotary valve, so that when the rotary motor 271 is started, the driving rod 272 can smoothly drive the rotary valve to rotate by a certain angle. The number of the rotary motor 271 and the drive rod 272 is two, and the two drive rods 272 are respectively inserted into the rotary valves of the reagent cartridge 210 from both sides. The two sides of the rotary valve are respectively rotated by the driving rods 272 at the corresponding sides, so that the rotary valve can accurately rotate for a certain angle.

When the kit 210 is installed, the microfluidic PCR chip 2103 on the kit 210 is sequentially inserted into the thermal lysis module 29, the magnet lifting module 28 and the temperature-variable amplification module 26, so that the kit 210 is reliably installed. The output of the rotary valve drive module 27 is inserted into the cartridge 210 so that the rotary valve can be accurately controlled. The magnetic lifting module 28 and the heating cracking module 29 are matched to complete the cracking and extracting functions of the nucleic acid, and finally the temperature-variable amplification module 26 is used for completing the amplification of the nucleic acid. After the kit 210 is installed in place, the control circuit board 22 controls the temperature-variable amplification module 26, the rotary valve driving module 27, the magnet lifting module 28 and the pyrolysis module 29 to operate, thereby realizing automatic molecular diagnosis.

Further, as shown in fig. 10, the magnet lifting module 28 includes a driving motor 281 mounted on the frame 21, the driving motor 281 is electrically connected to the control circuit board 22, a magnet 282 is connected to an output shaft of the driving motor 281, a magnet slot 283 is disposed on the magnet 282, and the microfluidic PCR chip 2103 passes through the magnet slot 283. When the driving motor 281 drives the magnet 282 to rise, the microfluidic PCR chip 2103 passes through the magnet slot 283, so that the separation of the magnetic beads inside the kit 210 is realized.

Further, a second touch sensor 284 for detecting whether the magnet 282 is lowered in place is mounted on the mounting bracket of the driving motor 281, and the second touch sensor 284 is electrically connected to the control circuit board 22. When the magnet 282 descends and presses the second touch sensor 284, the second touch sensor 284 sends a signal to the control circuit board 22, and the control circuit board 22 controls the driving motor 281 to stop.

Furthermore, a positioning frame 273 is further installed on the framework 21, a positioning disc 274 is installed on the driving rod 272, a valve position sensor 275 is sleeved on the positioning disc 274, and the valve position sensor 275 is installed on the positioning frame 273. The valve position sensor 275 on the positioning frame 273 determines the open position of the rotary valve by detecting the rotation angle of the positioning plate 274, facilitating automatic control. Therefore, the rotation condition of the rotary valve can be fed back by detecting through the valve position sensor 275, the rotary motor 271 is driven according to the feedback condition, and the accuracy of the rotary valve in place rotation is further improved. The positioning plate 274 is provided with a plurality of positioning grooves. The valve position sensor 275 determines the rotational angle of the positioning disk 274 by detecting the positioning slot or the fan-shaped positioning piece 245 between adjacent positioning slots, thereby determining the rotational angle of the rotary valve.

A third touch sensor 276 is installed on the inner side of the positioning frame 273, and the third touch sensor 276 is electrically connected with the control circuit board 22. When the reagent cartridge 210 is mounted in place, the end of the reagent cartridge 210 presses the third touch sensor 276, and the third touch sensor 276 transmits a signal to the control circuit board 22, so that whether the reagent cartridge 210 is mounted in place can be accurately judged by the third touch sensor 276.

As shown in fig. 12, 14 to 15, the optical signal emission detection module 23 includes an optical signal housing 2310, a bidirectional motor 231 is installed on the optical signal housing 2310, one output shaft of the bidirectional motor 231 is connected to a light source assembly 232 and an emission light filter wheel 233, and the other output shaft of the bidirectional motor 231 is connected to a reception light filter wheel 234; a light receiving cone 235 is arranged beside the emitting light filter disc 233, the light receiving cone 235 is connected with the receiving end of the variable temperature amplification module 26 through an optical fiber, a fiber collecting lens 236 is arranged beside the receiving light filter disc 234, the fiber collecting lens 236 is connected with the emitting end of the variable temperature amplification module 26 through an optical fiber, a detection probe 237 is arranged on one side of the receiving light filter disc 234 away from the fiber collecting lens 236, and a detection circuit board 238 is connected to the detection probe 237. The light source assembly 232 includes a light source plate 2321, the light source plate 2321 is installed on the output shaft of the bidirectional motor 231, six excitation light sources 2322 with different wavelengths are uniformly distributed on the light source plate 2321, six emission light filters corresponding to the excitation light sources 2322 are arranged on the emission light filter disc 233, and six reception light filters corresponding to reception light after the excitation light is amplified by the temperature-varying amplification module 26 are arranged on the reception light filter disc 234.

The bi-directional motor 231 drives the light source module 232, the emission light filter disk 233 and the reception light filter disk 234 to rotate, and when an excitation light source 2322 on the light source module 232 is aligned with the light receiving cone 235, the light emitted by the light source is filtered by the emission light filter with the wavelength corresponding to the light emitting light filter, and then is transmitted to the temperature varying amplification module 26 through the light receiving cone 235. The light passes through the reagent sample in the temperature-variable amplification module 26, and the temperature of the temperature-variable amplification module 26 is kept stable between ninety and sixty more degrees and cyclically switched so that the light passing through the excitation by the sample is enhanced. After receiving the excited light, the light condenser filters the stray light through the receiving light filter disc 234, and then the detection light is detected by the detection probe 237, and the excited light is analyzed through the detection circuit board 238. Whether the sample contains the corresponding substances is judged by judging whether the optical fiber is received or not, and the content of the corresponding substances in the sample is judged by judging the strength of the received optical fiber. The above steps are sequentially carried out by using exciting light with different wavelengths, and a plurality of substances in the sample can be respectively detected.

The bi-directional motor 231 synchronously drives the light source module 232, the transmitting light filter wheel 233 and the receiving light filter wheel 234 to rotate, so that the transmitting light filters and the receiving light filters are in one-to-one correspondence. When the fiber optic collection optic 236 receives the excited light, the receive filter wheel 234 needs to be adjusted individually. After the light source module 232 is adjusted by the bi-directional motor 231, the receiving optical filter aligned with the optical fiber condenser 236 corresponds to the wavelength of the received light. The present invention makes the adjustment of the receive optical filter wheel 234 more convenient and accurate.

Furthermore, a rotary positioning sensor 239 is further disposed beside the emission light filter disk 233, the rotary positioning sensor 239 is electrically connected to a control circuit board, and the control circuit board is electrically connected to the bi-directional motor 231. The rotational positioning sensor 239 can detect the rotational position of the light source plate 2321, thereby determining whether the corresponding excitation light source 2322 is rotated in place, and sending a signal to the control circuit board. When the excitation light source 2322 does not rotate in place, the control circuit board controls the bi-directional motor 231 to operate, so that the excitation light source 2322 rotates in place.

As shown in fig. 16, the temperature-variable amplification module 26 includes a module housing 263, a suspended heating block 261 is installed in the module housing 263, the light-receiving cone 235 is connected to a receiving end of the suspended heating block 261 through an optical fiber, the optical fiber condenser 236 is connected to an emitting end of the suspended heating block 261 through an optical fiber, and the peltier 262 is disposed on both sides of the suspended heating block 261. Heat sinks 264 are mounted on both sides of the module housing 263. The side of the heat sink 264 remote from the peltier 262 is fitted with a heat sink fan 265. The microfluidic PCR chip of the kit is inserted into the suspended heating block 261, and the suspended heating block 261 and the Peltier 262 can be controlled to be stable between ninety-many degrees and sixty-many degrees and can be kept stable and switched circularly, so that light of laser after the exciting light passes through the sample reagent can be exponentially enhanced, and the detection of the excited light is facilitated.

As shown in fig. 9, the thermal lysis module 29 is provided with a slot for inserting the microfluidic PCR chip 2103. The microfluidic PCR chip 2103 on the kit 210 is inserted into the slot of the thermal lysis module 29 to lyse the nucleic acid.

The piston lifting and film sealing puncturing device is part of a full-automatic PCR analysis system. As shown in fig. 1 to 4, the full-automatic PCR analysis system for molecular diagnosis using a microfluidic chip includes a plurality of analysis modules 1, wherein each analysis module 1 includes an analysis module core 2; the analysis module core 2 comprises a framework 21, a control circuit board 22 is arranged on the framework 21, and an optical signal emission detection module 23, a piston lifting module 24, a membrane sealing puncture column module 25, a variable temperature amplification module 26, a rotary valve driving module 27, a magnet lifting module 28 and a heating cracking module 29 which are all electrically connected with the control circuit board 22 are respectively arranged on the framework 21; the kit 210 is provided with a microfluidic PCR chip 2103, the microfluidic PCR chip 2103 is respectively inserted into the temperature-variable amplification module 26, the magnet lifting module 28 and the heating and cracking module 29, the output end of the rotary valve driving module 27 is inserted into the kit 210, the output ends of the piston lifting module 24 and the membrane sealing puncture column module 25 are both inserted into the kit 210, and the optical signal emission detection module 23 is electrically connected with the temperature-variable amplification module 26.

It should be noted that, in order to ensure that the system of the present invention is convenient to use, the present invention further includes a base 4, the plurality of analysis modules 1 are installed in the base 4, a human-computer interaction module 5 is further installed on the base 4, and the analysis modules 1 are respectively electrically connected with the human-computer interaction module 5.

The analysis module 1 further comprises a casing, the casing is provided with a fan 6, an interface board 7, a foot pad 8, an indicator lamp panel 9 and an indicator light guide post 10, except the analysis module movement 2, and the interface board 7 is electrically connected with a control circuit board 22 in the analysis module movement 2.

As shown in fig. 8 and 4, the cartridge 210 is provided with a plunger hole 2101 and a puncture hole 2102 at the upper side. The output end of piston lifting module 24 can be inserted into piston hole 2101, and the output end of membrane sealing puncture column module 25 can be inserted into puncture hole 2102. As shown in fig. 9, the microfluidic PCR chip 2103 is inserted into the temperature-variable amplification module 26, the magnet lifting module 28 and the thermal lysis module 29, respectively. The reagent cartridge 210 is further provided with a rotary valve operating hole 2104, and the output end of the rotary valve driving module 27 is inserted into the rotary valve operating hole 2104.

When the user uses the kit, the user firstly switches on the power supply and starts the kit for testing, after the user inputs the sample and the information of the kit 210, the sample is added into the sample position of the kit 210, then the cover of the kit 210 is closed, the corresponding detection position of the analysis system is inserted, and the test is started by clicking. All subsequent detection operations are automatically completed by the analysis system, and after the test is completed, the corresponding hatch door of the analysis system is opened, and the user waits for taking out the reagent kit 210 and clicks to complete the test to close the hatch door.

In the automatic operation process of the analysis system, the rotary valve driving module 27 drives the rotary valve to the position corresponding to the reagent cartridge 210, and the membrane sealing and puncturing module 25 punctures the liquid reagent sealing membrane in the reagent cartridge 210. The piston lifting module 24 then drives the rubber piston in the cartridge 210 to move, thereby effecting movement of the liquid. The rotary valve driving module 27 then drives the rotary valve to the desired position, draws the desired reagent through the piston and rotates the rotary valve to the desired position, squeezing the piston to expel the reagent, thus reciprocating the multiple reagent reactions. The magnetic lifting module 28 and the heating and cracking module 29 are matched to complete the cracking and extracting functions of the nucleic acid, and finally the temperature-variable amplification module 26 and the optical signal emission and detection module 23 are matched to complete the amplification and real-time optical signal detection of the nucleic acid. After the kit 210 is installed in place, the control circuit board 22 controls the optical signal emission detection module 23, the piston lifting module 24, the membrane sealing puncture column module 25, the temperature-variable amplification module 26, the rotary valve driving module 27, the magnet lifting module 28 and the pyrolysis module 29 to act, thereby realizing automatic molecular diagnosis.

As shown in fig. 4, in order to ensure that the reagent kit 210 is sealed during molecular diagnosis, the frame 21 is provided with a lifting door assembly 3, the lifting door assembly 3 comprises a lifting door motor 31 installed on the frame 21, an output shaft of the lifting door motor 31 is connected with a lifting door lead screw 32, the lifting door lead screw 32 is in threaded connection with a lifting platform 33, the lifting platform 33 is rotatably connected with a lifting door 34, and the lifting door 34 is lapped on the side wall of the frame 21. The door motor 31 can drive the door screw 32 to rotate, and the door screw 32 drives the lifting platform 33 to lift, so that the lifting platform 33 drives the lifting door 34 to lift. The lift gate 34 is raised before the reagent cartridge 210 is installed, and the lift gate 34 is lowered after the reagent cartridge 210 is installed. A positioning stopper 35 for limiting the elevating table 33 is further mounted on the frame 21.

The specific structures of the piston lifting module 24 and the seal piercing column module 25 are described below:

as shown in fig. 5 and 6, the piston lifting module 24 includes a lifting motor 241 mounted on the frame 21, an output end of the lifting motor 241 is connected with a lifting screw 242, the lifting screw 242 is connected with a lifting plate 243 through a thread, the lifting plate 243 is connected with a push rod 244 for pushing a rubber piston in the reagent kit 210 to move, and the push rod 244 is inserted into a piston hole 2101 of the reagent kit 210. The elevating motor 241 drives the elevating screw 242 to rotate, and the elevating screw 242 drives the elevating plate 243 to ascend or descend. When the lifting plate 243 descends, the pushing force on the lifting plate 243 extends into the reagent box 210 and drives the rubber piston in the reagent box 210 to move, so that the required reagent can be extracted to a required position by driving the piston to act.

In order to ensure the lifting plate 243 to move stably, two guide rods are further installed on the framework 21, two guide cylinders 247 are fixed on the lifting plate 243, and the guide cylinders 247 are sleeved on the guide posts 248. When the elevating plate 243 is driven by the elevating screw 242, the elevating plate 243 is restricted by the two guide posts 248 to be rotated, and the elevating plate 243 can be smoothly linearly elevated.

The membrane sealing and piercing module 25 includes a rotating plate 251, the rotating plate 251 is hinged to the frame 21, one end of the rotating plate 251 is lapped on the lifting plate 243, the other end of the rotating plate 251 is hinged to a piercing frame 252, and the piercing frame 252 is connected with a plurality of piercing rods 253 for piercing the liquid reagent sealing membrane in the reagent box 210. When the lifting plate 243 is lifted, the rotating plate 251 is pushed to rotate, so that the puncturing rod 253 on the rotating plate 251 can puncture the liquid reagent sealing membrane in the reagent kit 210.

When puncturing rod 253 is lifted, first touch sensor 255 is attached to frame 21 in order to limit puncture rack 252. When the puncture rack 252 rises to press the first touch sensor 255, the first touch sensor 255 sends a signal to the control circuit board 22, and the control circuit board 22 controls the lifting motor 241 to stop.

A plurality of return springs 254 are coupled to piercing carriage 252 for biasing piercing carriage 252. When the lifting plate 243 is lowered, the lifting plate 243 is separated from the rotating plate 251, and the return spring 254 pushes the puncture rack 252 and the puncture rod 253 upwards in the process of returning, so that the puncture rod 253 is prevented from staying in the reagent kit 210.

A positioning plate 245 is fixed on the lifting plate 243, three positioning sensors 246 are arranged on the moving path of the positioning plate 245, and the positioning sensors 246 are electrically connected with the control circuit board 22. Position sensors 246 include squeeze piston position sensor 2461, neutral position sensor 2462, and pierce position sensor 2463. When the positioning tab 245 moves to the position of the squeeze piston position sensor 2461, the push rod 244 moves to the lowermost end and the reagent in the piston is completely squeezed out. When the positioning tab 245 moves to the neutral sensor 2462 position, the piercing rod 253 and the push rod 244 are both separated from the reagent cartridge 210. When the positioning tab 245 is moved to the puncturing position sensor 2463, the puncturing rod 253 is in a position to puncture the liquid reagent sealing membrane. The squeeze piston position sensor 2461, the middle position sensor 2462 and the puncture position sensor 2463 are all electrically connected to the control circuit board 22, and when any one of the positioning sensors 246 is triggered by the positioning piece 245, the positioning sensor 246 sends a signal to the control circuit board 22, and the control circuit board 22 controls the stop or start of the lifting motor 241.

As shown in fig. 13, the frame 21 is provided with a stopper groove 211 for stopping the puncture rack 252. The limiting groove 211 can limit the puncture frame 252, and ensure that the puncture frame 252 can accurately move along a straight line, thereby ensuring that the puncture rod 253 can accurately puncture the sealing film.

The invention is not limited to the above alternative embodiments, and any other various forms of products can be obtained by anyone in the light of the present invention, but any changes in shape or structure thereof, which fall within the scope of the present invention as defined in the claims, fall within the scope of the present invention.