阅读说明：本技术 封闭式微生物培养系统 (Closed microorganism culture system ) 是由 刘青松 于 2021-08-31 设计创作，主要内容包括：本申请涉及微生物培养技术领域,尤其是涉及一种封闭式微生物培养系统。封闭式微生物培养系统,包括均为密闭容器的反应器和补料器,以及压滤采收装置和液路循环模块。补料器用于存放微生物和营养液形成的混合液,液路循环模块包括供液管路、采收管路、排放管路和回收管路；补料器内的混合液能够通过供液管路输送至反应器内,以在反应器内进行微生物培养；反应器内的混合液能够通过采收管路输送至补料器内；补料器内的混合液经沉淀后能够通过排放管路输送至压滤采收装置进行压滤,且压滤后得到的混合液能够通过回收管路输送至补料器；从而实现对营养液的循环利用,降低微生物培养成本,同时也减轻了后续废水处理的负担。(The application relates to the technical field of microbial culture, in particular to a closed microbial culture system. The closed microbial culture system comprises a reactor and a material supplementing device which are all closed containers, a filter pressing and harvesting device and a liquid path circulation module. The liquid path circulation module comprises a liquid supply pipeline, a collection pipeline, a discharge pipeline and a recovery pipeline; the mixed liquid in the material supplementing device can be conveyed into the reactor through a liquid supply pipeline so as to carry out microbial culture in the reactor; the mixed liquid in the reactor can be conveyed into the material supplementing device through a collecting pipeline; the mixed liquid in the material supplementing device can be conveyed to a filter pressing and collecting device for filter pressing through a discharge pipeline after being precipitated, and the mixed liquid obtained after filter pressing can be conveyed to the material supplementing device through a recovery pipeline; thereby realizing the cyclic utilization of the nutrient solution, reducing the culture cost of the microorganisms and simultaneously lightening the burden of the subsequent wastewater treatment.)

1. A closed microorganism culture system is characterized by comprising a reactor, a material supplementing device, a filter-pressing and harvesting device and a liquid path circulation module;

the reactor and the material supplementing device are both closed containers, and the material supplementing device is used for storing mixed liquid formed by microorganisms and nutrient solution;

the liquid path circulation module comprises a liquid supply pipeline, a harvesting pipeline, a discharge pipeline and a recovery pipeline;

the mixed liquid in the feeder can be conveyed into the reactor through the liquid supply pipeline so as to culture microorganisms in the reactor;

the mixed liquor in the reactor can be conveyed into the material supplementing device through the recovery pipeline, so that the mixed liquor is subjected to preliminary precipitation in the material supplementing device;

the sediment in the material supplementing device can be conveyed to the filter-pressing recovery device through the discharge pipeline, so that the mixed liquid is subjected to filter pressing through the filter-pressing recovery device, and the mixed liquid obtained after filter pressing can be conveyed to the material supplementing device through the recovery pipeline.

2. The closed microbial cultivation system of claim 1, wherein the liquid supply pipeline, the recovery pipeline and the recovery pipeline are respectively provided with a liquid delivery pump;

the discharge pipeline is provided with a first electric valve to open or close the discharge pipeline.

3. The closed microbial cultivation system of claim 1, wherein the liquid path circulation module further comprises a primary overflow pipe;

a first overflow port is arranged at the preset height of the barrel of the reactor and communicated with the material supplementing device through the primary overflow pipe, so that the mixed liquid in the reactor can overflow into the material supplementing device;

the closed microorganism culture system also comprises a liquid storage device, clear liquid in the filter-pressing harvesting device can be discharged into the liquid storage device, and the liquid storage device can be communicated with the material supplementing device through the recovery pipeline;

the liquid path circulation module further comprises a second-stage overflow pipe, a second overflow port is arranged at the preset height of the barrel of the material supplementing device, and the second overflow port is communicated with the liquid storage device through the second-stage overflow pipe, so that the mixed liquid in the material supplementing device can overflow into the liquid storage device.

4. The closed microbial cultivation system of claim 1, further comprising a gas circuit circulation module;

the gas circuit circulating module comprises a gas inlet main pipe, a first gas inlet pipeline and a second gas inlet pipeline;

the reactor with be provided with aeration mixing device in the feed supplement ware respectively, inlet manifold's inlet end is used for being linked together with air feeder, inlet manifold's the end of giving vent to anger respectively with first inlet line and second inlet line are linked together, with through first inlet line way is to aeration mixing device transport gas in the reactor, through second inlet line way is to aeration mixing device transport gas in the feed supplement ware.

5. The closed microbial cultivation system of claim 4, wherein the air inlet manifold is provided with a dryer and a second electric valve;

and the first air inlet pipeline and the second air inlet pipeline are respectively provided with a filter, a gas flowmeter and a third electric valve.

6. The closed microbiological culture system of claim 4 wherein said gas circuit circulation module further comprises a first gas exhaust circuit and a second gas exhaust circuit;

the reactor with be provided with the tail gas discharge port on the moisturizing ware respectively, the tail gas discharge port of reactor pass through first exhaust pipe with the end of giving vent to anger of air inlet manifold is linked together, the tail gas discharge port of moisturizing ware pass through the second exhaust pipe with the end of giving vent to anger of air inlet manifold is linked together.

7. The closed microbial cultivation system of claim 6, wherein the gas circuit circulation module further comprises a third exhaust gas circuit;

the first exhaust pipeline and the second exhaust pipeline are communicated with the air inlet main pipe through the third exhaust pipeline;

a gas-liquid separator and a gas buffer tank are sequentially arranged on the third exhaust pipeline along the flowing direction of the gas in the third exhaust pipeline;

a liquid recovery device is further arranged at the liquid outlet of the gas-liquid separator, and liquid in the gas-liquid separator can be discharged into the liquid recovery device for collection;

and an exhaust main pipe is arranged on the gas buffer tank, and a fourth electric valve is arranged on the exhaust main pipe.

8. The closed microbial cultivation system of claim 4, wherein the reactor is provided with a temperature control device;

a jacket cylinder is sleeved and mounted on the outer side of the cylinder of the reactor, the jacket cylinder is communicated with the temperature control device to form a circulating loop, and circulating water with a preset temperature is conveyed into the jacket cylinder through the temperature control device;

the temperature control device comprises a circulating water pump, a heater, a refrigerator and a circulating water tank;

the water outlet of the jacket cylinder is communicated with the water inlet of the circulating water tank through a first circulating pipe, and the water inlet of the jacket cylinder is communicated with the water outlet of the circulating water tank through a second circulating pipe; the heater, the refrigerator and the circulating water pump are connected in series to the second circulating pipe.

9. The closed microorganism culture system according to claim 1, further comprising a high temperature steam sterilization device;

the reactor is provided with a steam inlet which is communicated with the high-temperature steam sterilization device so that the high-temperature steam sterilization device can introduce high-temperature steam into the reactor;

the steam inlet further comprises a pressure control valve to control the sterilization pressure within the reactor;

the feeder is provided with a steam discharge port, and the steam discharge port is provided with a steam discharge valve.

10. The closed microbial cultivation system of claim 1, wherein the reactor is further provided with a sample ectopic detection device;

the sample ectopic detection device comprises a sample detection cavity, a first circulating pump and a second circulating pump;

the first circulating pump can with the mixed liquid suction in the reactor sample detection chamber, just the mixed liquid in the sample detection chamber can be sent back to the reactor via the second circulating pump.

11. The closed microbial cultivation system of claim 8, wherein an acid-base adjusting device is further arranged on the reactor;

the acid-base adjusting device comprises an acid liquid storage tank, an acid liquid feeding pump, an alkali liquid storage tank and an alkali liquid feeding pump;

the acid liquor storage tank can convey acid liquor into the reactor through the acid liquor feed pump, and the alkali liquor storage tank can convey alkali liquor into the reactor through the alkali liquor feed pump.

12. The closed microbial cultivation system of claim 11, wherein the head of the feeder is tapered;

the top of the material supplementing device is provided with a material supplementing opening, the microorganisms and the nutrient solution can be added into the material supplementing device through the material supplementing opening, and the recovery pipeline is communicated with the material supplementing device through the material supplementing opening;

a stirring device is arranged in the reactor and is used for stirring the mixed liquid in the reactor;

the reactor is provided with a full-spectrum lighting device to provide light energy for culturing microorganism species needing illumination in the reactor;

the reactor is provided with a window for observing the inside of the reactor, and the side wall of the reactor is provided with a wall scraper.

13. The closed microbial cultivation system of claim 12, further comprising a cultivation parameter on-line monitoring module;

the culture parameter online monitoring module is used for monitoring the liquid level height, the pH value, the temperature, the DO value, the illumination intensity, the turbidity and the chlorophyll fluorescence in the reactor.

14. The closed microbial cultivation system of claim 13 further comprising a control system;

the culture parameter online monitoring module is in communication connection with the control system;

the temperature control device, the full-spectrum lighting device, the acid-base adjusting device, the liquid path circulating module, the gas path circulating module and the stirring device of the reactor are all in communication connection with the control system.

Technical Field

The application relates to the technical field of microbial culture, in particular to a closed microbial culture system.

Background

The application of microorganisms has penetrated into various aspects of food, medicine, environmental protection, fuel oil and the like, and the microbial reactor ensures that related biochemical reactions are carried out under controllable conditions and obtains an optimal reaction result, and is one of key factors for promoting the large-scale and industrialized application of the microorganisms; the microbial reactor can be divided into a closed reactor and an open type reactor according to the degree of closure, the closed reactor is mainly used for fermentation industry, environmental protection treatment and other aspects, but the prior closed microbial reactor does not consider the cyclic utilization of nutrient solution, so that the culture cost is increased, and the burden of subsequent wastewater treatment is also increased.

Disclosure of Invention

The invention aims to provide a closed microorganism culture system, which can recycle nutrient solution.

The invention provides a closed microorganism culture system, which comprises a reactor, a material supplementing device, a filter pressing and harvesting device and a liquid path circulation module, wherein the material supplementing device is arranged on the reactor; the reactor and the material supplementing device are both closed containers, and the material supplementing device is used for storing mixed liquid formed by microorganisms and nutrient solution; the liquid path circulation module comprises a liquid supply pipeline, a harvesting pipeline, a discharge pipeline and a recovery pipeline; the mixed liquid in the feeder can be conveyed into the reactor through the liquid supply pipeline so as to culture microorganisms in the reactor; the mixed liquor in the reactor can be conveyed into the material supplementing device through the recovery pipeline for preliminary precipitation; the sediment in the material supplementing device can be conveyed to the filter-pressing recovery device through the discharge pipeline, so that the mixed liquid is subjected to filter pressing through the filter-pressing recovery device, and the mixed liquid obtained after filter pressing can be conveyed to the material supplementing device through the recovery pipeline.

Further, the liquid supply pipeline, the recovery pipeline and the recovery pipeline are respectively provided with a liquid delivery pump; the discharge pipeline is provided with a first electric valve to open or close the discharge pipeline.

Further, the liquid path circulating module also comprises a primary overflow pipe; a first overflow port is arranged at the preset height of the barrel of the reactor and communicated with the material supplementing device through the primary overflow pipe, so that the mixed liquid in the reactor can overflow into the material supplementing device; the closed microorganism culture system also comprises a liquid storage device, clear liquid in the filter-pressing harvesting device can be discharged into the liquid storage device, and the liquid storage device can be communicated with the material supplementing device through the recovery pipeline;

the liquid path circulation module further comprises a second-stage overflow pipe, a second overflow port is arranged at the preset height of the barrel of the material supplementing device, and the second overflow port is communicated with the liquid storage device through the second-stage overflow pipe, so that the mixed liquid in the material supplementing device can overflow into the liquid storage device.

Further, the closed microorganism culture system also comprises an air path circulation module; the gas circuit circulating module comprises a gas inlet main pipe, a first gas inlet pipeline and a second gas inlet pipeline; the reactor with be provided with aeration mixing device in the feed supplement ware respectively, inlet manifold's inlet end is used for being linked together with the air feeder, inlet manifold's the end of giving vent to anger respectively with ground first inlet line and second inlet line are linked together, with pass through first inlet line way is to aeration mixing device transport gas in the reactor, through second inlet line way is to aeration mixing device transport gas in the feed supplement ware.

Further, a dryer and a second electric valve are arranged on the air inlet main pipe; and the first air inlet pipeline and the second air inlet pipeline are respectively provided with a filter, a gas flowmeter and a third electric valve.

Further, the gas circuit circulating module also comprises a first exhaust pipeline and a second exhaust pipeline; the reactor with be provided with the tail gas discharge port on the moisturizing ware respectively, the tail gas discharge port of reactor pass through first exhaust pipe with the end of giving vent to anger of air inlet manifold is linked together, the tail gas discharge port of moisturizing ware pass through the second exhaust pipe with the end of giving vent to anger of air inlet manifold is linked together.

Further, the gas circuit circulating module further comprises a third gas exhaust pipeline; the first exhaust pipeline and the second exhaust pipeline are communicated with the air inlet main pipe through the third exhaust pipeline; a gas-liquid separator and a gas buffer tank are sequentially arranged on the third exhaust pipeline along the flowing direction of the gas in the third exhaust pipeline; a liquid recovery device is further arranged at the liquid outlet of the gas-liquid separator, and liquid in the gas-liquid separator can be discharged into the liquid recovery device for collection; an exhaust main pipe is arranged on the gas buffer, and a fourth electric valve is arranged on the exhaust main pipe.

Furthermore, a temperature control device is arranged on the reactor; a jacket cylinder is sleeved and mounted on the outer side of the cylinder of the reactor, the jacket cylinder is communicated with the temperature control device to form a circulating loop, and circulating water with a preset temperature is conveyed into the jacket cylinder through the temperature control device; the temperature control device comprises a circulating water pump, a heater, a refrigerator and a circulating water tank; the water outlet of the jacket cylinder is communicated with the water inlet of the circulating water tank through a first circulating pipe, and the water inlet of the jacket cylinder is communicated with the water outlet of the circulating water tank through a second circulating pipe; the heater, the refrigerator and the circulating water pump are connected in series to the second circulating pipe.

Further, the closed microorganism culture system also comprises a high-temperature steam sterilization device; the reactor is provided with a steam inlet which is communicated with the high-temperature steam sterilization device so that the high-temperature steam sterilization device can introduce high-temperature steam into the reactor; the steam inlet further comprises a pressure control valve to control the sterilization pressure within the reactor; the feeder is provided with a steam discharge port, and the steam discharge port is provided with a steam discharge valve.

Further, a sample ectopic detection device is also arranged on the reactor; the sample ectopic detection device comprises a sample detection cavity, a first circulating pump and a second circulating pump; the first circulating pump can with the mixed liquid suction in the reactor the sample detects the intracavity, just the mixed liquid in the sample detects the intracavity can be sent back to the reactor via the second circulating pump.

Further, an acid-base adjusting device is arranged on the reactor; the acid-base adjusting device comprises an acid liquid storage tank, an acid liquid feeding pump, an alkali liquid storage tank and an alkali liquid feeding pump; the acid liquor storage tank can convey acid liquor into the reactor through the acid liquor feed pump, and the alkali liquor storage tank can convey alkali liquor into the reactor through the alkali liquor feed pump.

Furthermore, the end socket of the material supplementing device is conical; the top of the material supplementing device is provided with a material supplementing opening, the microorganisms and the nutrient solution can be added into the material supplementing device through the material supplementing opening, and the recovery pipeline is communicated with the material supplementing device through the material supplementing opening; a stirring device is arranged in the reactor and is used for stirring the mixed liquid in the reactor; the reactor is provided with a full-spectrum lighting device to provide illumination for the culture of microorganism species needing illumination in the reactor; the reactor is provided with a window for observing the inside of the reactor, and the side wall of the reactor is provided with a wall scraper.

Furthermore, the closed microorganism culture system also comprises a culture parameter online monitoring module; the culture parameter online monitoring module is used for monitoring the pH value, the temperature, the DO value, the illumination intensity, the turbidity and the chlorophyll fluorescence in the reactor.

Further, the closed microorganism culture system also comprises a control system; the culture parameter online monitoring module is in communication connection with the control system; the temperature control device, the full-spectrum lighting device, the acid-base adjusting device, the liquid path circulating module, the gas path circulating module and the stirring device of the reactor are all in communication connection with the control system.

Compared with the prior art, the invention has the beneficial effects that:

the invention provides a closed microorganism culture system which comprises a reactor and a feeder which are all closed containers, a filter-pressing and harvesting device and a liquid path circulation module. The liquid path circulation module comprises a liquid supply pipeline and a recovery pipeline which are arranged between the reactor and the material supplementing device; the mixed liquid in the material supplementing device can be conveyed into the reactor through the liquid supply pipeline so as to culture the microorganisms in the reactor, and a proper culture environment is provided for the culture of the microorganisms through the reactor. After the microorganisms finish a culture period in the reactor, the mixed liquid in the reactor can be conveyed into the material supplementing device through the collecting pipeline, and the mixed liquid formed after finishing the culture period, namely the product mixed liquid, is collected through the material supplementing device.

The liquid path circulation module also comprises a discharge pipeline and a recovery pipeline which are arranged between the material supplementing device and the filter-pressing recovery device; the product mixed liquor is precipitated in the material supplementing device for a period of time and then can be discharged into a filter pressing collecting device through a discharge pipeline for filter pressing so as to further separate microorganisms from nutrient solution; the liquid outlet of filter-pressing harvesting device is linked together through the feed supplement mouth of recovery pipeline with feed supplement ware upper end, and the nutrient solution that obtains after the filter-pressing of filter-pressing harvesting device can carry back the feed supplement ware through the recovery pipeline in, carry out cyclic utilization.

Therefore, through setting up the circulation circuit that supplies the liquid pipeline and gather the pipeline and form mixed liquid between feed supplement ware and reactor, also form the circulation circuit of mixed liquid between feed supplement ware and filter-pressing harvesting device through setting up discharge line and recovery pipeline, can carry out cyclic utilization to the nutrient solution, reduce the microorganism and cultivate the cost, also alleviateed subsequent waste water treatment's burden simultaneously.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.

FIG. 1 is a schematic flow diagram of a closed microbial cultivation system according to an embodiment of the present invention;

FIG. 2 is a schematic flow chart of a liquid circuit circulation module according to an embodiment of the present invention;

fig. 3 is a schematic flow chart of the gas circuit circulation module according to the embodiment of the present invention.

Reference numerals:

1-a reactor, 11-a circulating water pump, 12-a heater, 13-a refrigerator, 14-a circulating water tank, 15-a high-temperature steam sterilization device, 16-a stirring device, 17-a full spectrum lighting device, 18-a window, 19-a wall scraper, 2-a material supplementing device, 21-a steam discharge valve, 3-a filter pressing and harvesting device, 31-a liquid storage device, 4-a liquid path circulation module, 41-a liquid supply pipeline, 42-a harvesting pipeline, 43-a discharge pipeline, 44-a recovery pipeline, 45-a liquid delivery pump, 46-a first electric valve, 47-a primary overflow pipe, 48-a secondary overflow pipe, 5-a gas path circulation module, 51-a gas inlet header pipe, 52-a first gas inlet pipeline, 53-a second gas inlet pipeline, 54-a dryer, 55-a second electric valve, 56-a filter, 57-a gas flowmeter, 58-a third electric valve, 59-a first exhaust pipeline, 510-a second exhaust pipeline, 511-a third exhaust pipeline, 512-a gas-liquid separator, 513-a gas buffer tank, 514-a liquid recovery device, 515-an exhaust manifold, 516-a fourth electric valve, 6-an aeration blending device, 7-a sample ex-situ detection device, 8-a pH value adjusting device and 9-a control system.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention.

The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention.

All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

A closed microbial cultivation system according to some embodiments of the present application is described below with reference to fig. 1 to 3.

The application provides a closed microbial cultivation system, as shown in fig. 1 and 2, including reactor 1 and feed supplement ware 2, filter-pressing harvesting device 3 and set up and be used for carrying out the liquid circuit circulation module 4 that the liquid circuit communicates to the three between the three that are airtight container.

The material supplementing device 2 can be added with microorganisms and nutrient solution; preferably, the top of the material supplementing device 2 is provided with a material supplementing port, and the material supplementing device 2 can be communicated with a feeding device of microorganisms and nutrient solution through the material supplementing port so as to add the microorganisms and the nutrient solution into the material supplementing device 2 through the material supplementing port; meanwhile, after one culture period is finished, nutrients can be added into the material supplementing device 2 through the material supplementing opening so as to blend the components of the nutrient solution in the material supplementing device 2.

As shown in fig. 2, the liquid circuit circulation module 4 comprises a liquid supply circuit 41, a recovery circuit 42 and a primary overflow pipe 47 arranged between the reactor 1 and the material feeder 2; wherein both ends of the liquid supply pipeline 41 are respectively communicated with the discharge hole of the material supplementing device 2 and the feed inlet of the reactor 1, mixed liquid formed by microorganisms and nutrient solution in the material supplementing device 2 can be conveyed into the reactor 1 through the liquid supply pipeline 41 so as to culture the microorganisms in the reactor 1, and a proper culture environment is provided for the culture of the microorganisms through the reactor 1. Preferably, a liquid delivery pump 45 is arranged on the liquid supply line 41, so that the mixed liquid in the feeding device 2 is pumped into the reactor 1 through the liquid delivery pump 45.

In this embodiment, as shown in fig. 2, a first overflow port is provided at a predetermined height of the barrel of the reactor 1, one end of the primary overflow pipe 47 is communicated with the first overflow port, and the other end of the primary overflow pipe 47 is inserted into the inside of the material replenishing device 2; when the liquid level of the mixed liquid in the reactor 1 reaches the height of the first overflow port, the mixed liquid in the reactor 1 can overflow into the material supplementing device 2 through the first overflow port and the primary overflow pipe 47, so that the preset bearing capacity of the reactor 1 is not exceeded.

The upper end of the material supplementing device 2 is provided with a collecting port, and two ends of a collecting pipeline 42 are respectively communicated with the collecting port of the material supplementing device 2 and the discharge port of the reactor 1; after the microorganisms have completed one cultivation cycle in the reactor 1, the mixed liquor in the reactor 1 can be transferred into the feeder 2 through the recovery pipe 42; preferably, another liquid delivery pump 45 is disposed on the recovery pipeline 42 to pump the mixed liquid in the reactor 1 into the material supplementing device 2, collect the mixed liquid formed after completing one culture cycle, i.e. the product mixed liquid, through the material supplementing device 2, and allow the product mixed liquid to settle in the material supplementing device 2; after precipitation, the microorganisms in the product mixed solution can precipitate below the material supplementing device 2, and a supernatant is formed above the microorganisms; thereby realizing the first-stage concentration and harvesting of the microorganisms through the precipitation of the material supplementing device on the microorganisms.

Preferably, as shown in fig. 2, the end socket at the lower end of the feeding device 2 is a conical end socket, so that the mixed liquid can be precipitated in the feeding device 2.

Therefore, a liquid phase circulation loop is formed between the reactor 1 and the material supplementing device 2 by providing a primary overflow pipe 47, a liquid supply pipe 41 and a recovery pipe 42 each provided with a liquid transfer pump 45 between the reactor 1 and the material supplementing device 2.

The liquid path circulation module 4 also comprises a discharge pipeline 43, a recovery pipeline 44 and a secondary overflow pipe 48 which are arranged between the material supplementing device 2 and the filter-pressing recovery device 3; the both ends of emission pipeline 43 are linked together with the discharge gate of feed supplement ware 2 and the feed inlet of filter-pressing recovery device 3 respectively, and the product mixed liquid can discharge the microorganism precipitate of lower floor that obtains after a period of deposit in feed supplement ware 2 to carry out the filter-pressing in filter-pressing recovery device 3 through emission pipeline 43 to carry out further separation to microorganism and nutrient solution, realize carrying out the secondary concentration to the microorganism and gather.

Preferably, as shown in fig. 2, the filter-press recovery device 3 is arranged below the material supplementing device 2, so that the mixed liquor in the material supplementing device 2 can flow into the filter-press recovery device 3 through the discharge pipeline 43 under the action of the potential difference; a first electric valve 46 is provided in the discharge line 43, and the discharge line 43 can be opened or closed by the first electric valve 46.

The liquid outlet of filter-pressing harvesting device 3 is linked together through the feed supplement mouth of recovery pipeline 44 with feed supplement ware 2 upper end, and preferably, the below of filter-pressing harvesting device 3 is provided with inclosed reservoir 31, and the liquid outlet of filter-pressing harvesting device 3 is linked together with the inlet of reservoir 31, and the nutrient solution that obtains after the filter-pressing of filter-pressing harvesting device 3 can be discharged and deposit in reservoir 31, and the concentrated microorganism that obtains after the filter-pressing then accessible subsequent operation carries out the purpose result and draws.

The liquid outlet of the liquid storage device 31 is communicated with the recovery pipeline 44, so that the nutrient solution in the liquid storage device 31 can be conveyed back into the material supplementing device 2 through the recovery pipeline 44; preferably, a liquid delivery pump 45 is also disposed on the recycling pipeline 44 to pump the nutrient solution in the liquid reservoir 31 back into the feeder 2, so that the nutrient solution is recycled through the feeder 2, and the microorganism is cultured by using the nutrient solution again, thereby recycling the nutrient solution.

It should be noted that, when the nutrient solution stored in the material supplementing device 2 is reused to culture microorganisms, nutrients can be added into the material supplementing device 2 through the material supplementing port to blend the components of the nutrient solution in the material supplementing device, so that the components of the nutrient solution in the material supplementing device can meet the culture requirements of the microorganisms.

Preferably, as shown in FIG. 2, a secondary overflow pipe 48 is arranged between the material feeder 2 and the reservoir 31, a second overflow port is arranged at a predetermined height of the cylinder of the material feeder 2, one end of the secondary overflow pipe 48 is communicated with the second overflow port, and the other end of the secondary overflow pipe 48 is inserted into the reservoir 31; when the liquid level of the mixed liquid in the material supplementing device 2 reaches the height of the second overflow port, the mixed liquid in the material supplementing device 2 can overflow into the liquid storage device 31 through the second overflow port and the second-stage overflow pipe 48 for temporary storage, so as to ensure that the mixed liquid in the material supplementing device 2 does not exceed the preset bearing capacity of the material supplementing device 2.

Therefore, can guarantee reactor 1 and feed supplement ware 2 not to overload respectively through setting up one-level overflow pipe 47 and second grade overflow pipe 48, and can arrange unnecessary mixed liquid and keep in reservoir 31, when guaranteeing whole culture system's leakproofness, also avoid the waste of nutrient solution, can carry out cyclic utilization at subsequent microbial cultivation in-process to the nutrient solution in the reservoir 31.

In conclusion, the material supplementing device 2 with the functions of material storage, material supplementing and precipitation harvesting is arranged, liquid path circulation is formed between the material supplementing device 2 and the reactor 1, and liquid path circulation is also formed between the material supplementing device 2 and the filter pressing harvesting device 3 and the liquid storage device 31, so that the microorganisms can be cultured in a totally-enclosed manner, the condition that no mixed bacteria interference exists in the culture process is ensured, and the product quality is controllable; meanwhile, the nutrient solution can be recycled, the microbial culture cost is reduced, and the burden of subsequent wastewater treatment is reduced.

In one embodiment of the present application, preferably, as shown in fig. 1 and 3, the closed microorganism cultivation system further comprises a gas circuit circulation module 5.

The air path circulation module 5 comprises an air inlet manifold 51 with an air inlet end communicated with the air supply equipment, and a first air inlet pipeline 52 and a second air inlet pipeline 53 which are respectively communicated with an air outlet end of the air inlet manifold 51. The bottoms of the reactor 1 and the material supplementing device 2 are respectively provided with an aeration blending device 6, preferably, the aeration blending device 6 is an annular pipeline, and a plurality of air outlets with preset apertures are formed in the annular pipeline. The first air inlet pipeline 52 and the aeration and uniform mixing device 6 in the reactor 1 can introduce air into the reactor 1 through the aeration and uniform mixing device 6 and form bubbles in the reactor 1, so that the mixed liquid in the reactor 1 can be subjected to aeration and uniform mixing or the mixed liquid can be used for carrying out gas stripping on microorganisms in the reactor 1. Preferably, the aeration and uniform mixing device 6 in the material supplementing device 2 is communicated with the second air inlet pipeline 53, and air can enter the material supplementing device 2 through the aeration and uniform mixing device 6 in the material supplementing device 2 so as to be used for carrying out aeration and uniform mixing on mixed liquid in the material supplementing device 2 or carrying out air stripping on microorganisms in the material supplementing device 2.

In one embodiment of the present application, preferably, as shown in fig. 3, the first air intake pipeline 52 and the second air intake pipeline 53 are further provided with a filter 56, a gas flow meter 57 and a third electric valve 58, respectively; the air enters the corresponding aeration blending device 6 after sequentially passing through the filter 56, the gas flow meter 57 and the third electric valve 58, the air entering the aeration blending device 6 can be filtered through the filter 56 to filter out bacteria and particles, and the air inlet flow is controlled through the gas flow meter 57; the third electric valve 58 is used for opening or closing the communication between the aeration and uniform mixing device 6 and the corresponding first air inlet pipeline 52 or second air inlet pipeline 53.

Preferably, as shown in fig. 3, the intake manifold 51 is provided with a second electric valve 55, and the second electric valve 55 can open or close communication between the intake manifold 51 and the air supply equipment.

In this embodiment, preferably, as shown in fig. 3, when the closed microbial cultivation system of the present application is used for development of deuterated biomolecules, a dryer 54 may be further disposed on the air intake manifold 51 to dry the air entering the air intake manifold 51, remove moisture contained in the air, and prevent water vapor in the air from entering the reactor 1 to exchange hydrogen and deuterium with heavy water in the reactor 1, which affects the quality of the product.

In one embodiment of the present application, preferably, as shown in fig. 3, the gas circuit circulation module 5 further includes a first exhaust circuit 59, a second exhaust circuit 510, and a third exhaust circuit 511. The top ends of the reactor 1 and the material supplementing device 2 are respectively provided with a tail gas discharge port, the tail gas discharge port of the reactor 1 is communicated with a first exhaust pipeline 59, the tail gas discharge port of the material supplementing device 2 is communicated with a second exhaust pipeline 510, and the first exhaust pipeline 59 and the second exhaust pipeline 510 are respectively connected to a third exhaust pipeline 511 and are communicated with the gas outlet end of the gas inlet main pipe 51 through the third exhaust pipeline 511.

Preferably, as shown in fig. 3, the third exhaust pipeline 511 is provided with a gas-liquid separator 512 and a gas buffer tank 513; culture tail gas generated by the material supplementing device 2 and the reactor 1 in the microbial culture process can enter the gas-liquid separator 512 for gas-liquid separation; a liquid recovery device 514 is arranged at the liquid outlet of the gas-liquid separator 512, the mixed liquid separated in the gas-liquid separator 512 can be discharged into the liquid recovery device 514 for temporary storage and collection, the gas outlet of the gas-liquid separator 512 is communicated with the gas buffer tank 513, and the culture tail gas after gas-liquid separation can enter the gas buffer tank 513 for storage; when the feeder 2 or the reactor 1 needs to be uniformly aerated or blown off, the gas in the gas buffer tank 513 can enter the first gas inlet pipeline 52 and the second gas inlet pipeline 53 without opening the gas inlet main pipe 51, so as to recycle the culture tail gas.

Preferably, as shown in fig. 3, an exhaust manifold 515 is further disposed on the gas buffer tank 513, the exhaust manifold 515 can be communicated with the atmosphere, and a fourth electric valve 516 is disposed on the exhaust manifold 515 and opens and closes the exhaust manifold 515. When the gas in the gas circuit circulation module 5 needs to be refreshed, the second electric valve 55 on the gas inlet manifold 51 and the fourth electric valve 516 on the gas outlet manifold 515 can be opened simultaneously to refresh the air in the closed microorganism culture system.

In one embodiment of the present application, preferably, as shown in fig. 1, a temperature control device is arranged on the reactor 1, and the temperature control device comprises a circulating water pump 11, a heater 12, a refrigerator 13 and a circulating water tank 14; a jacket cylinder is sleeved on the outer side of the cylinder of the reactor 1, the water outlet of the jacket cylinder is communicated with the water inlet of the circulating water tank 14 through a first circulating pipe, and the water inlet of the jacket cylinder is communicated with the water outlet of the circulating water tank 14 through a second circulating pipe; the heater 12, the refrigerator 13 and the circulating water pump 11 are connected in series on the second circulating pipe; therefore, the jacket cylinder is communicated with the temperature control device to form a circulation loop, circulating water with a preset temperature can be conveyed into the jacket cylinder through the temperature control device, the temperature inside the reactor 1 is further controlled, and a proper temperature is provided for the culture of microorganisms.

In one embodiment of the present application, preferably, as shown in fig. 1, the closed microorganism cultivation system further includes a high temperature steam sterilization device 15, the high temperature steam sterilization device 15 being capable of outputting high temperature steam; the reactor 1 is provided with a steam inlet which is communicated with the high-temperature steam sterilization device 15, the material supplementing device 2 is provided with a steam discharge port, and the steam discharge port is provided with a steam discharge valve 21; when the microorganism is cultured by the closed microorganism culture system, the whole system needs to be sterilized, high-temperature steam can be conveyed into the reactor 1 through the high-temperature steam sterilization device 15, and then the high-temperature steam is discharged through a steam discharge port at the upper end of the material supplementing device 2, so that the high-temperature steam flows through the whole system, and the whole system is sterilized by the high-temperature steam.

In one embodiment of the present application, preferably, as shown in fig. 1, the closed microorganism culture system further comprises a sample ectopic detection device 7; the sample ectopic detection device 7 comprises a sample detection cavity, a first circulating pump and a second circulating pump, wherein a liquid inlet of the sample detection cavity is communicated with the reactor 1 through the first circulating pump, a liquid outlet of the sample detection cavity is communicated with the reactor 1 through the second circulating pump, mixed liquid in the reactor 1 can be pumped out to the sample detection cavity through the first circulating pump, and the mixed liquid in the sample detection cavity can be pumped back to the reactor 1 through the second circulating pump; therefore, when the mixed liquid in the reactor 1 needs to be detected, the mixed liquid in the reactor 1 can be sampled through the sample detection cavity for collecting the spectral data and the like of the sample.

In one embodiment of the present application, preferably, as shown in fig. 1, the closed microbial cultivation system further comprises an acid-base regulation device; the acid-base adjusting device comprises an acid liquid storage tank, an acid liquid feeding pump, an alkali liquid storage tank and an alkali liquid feeding pump; the acid liquor storage tank can convey acid liquor into the reactor 1 through the acid liquor feeding pump, and the alkali liquor storage tank can convey alkali liquor into the reactor 1 through the alkali liquor feeding pump, so that the pH value (hydrogen ion concentration index) of the mixed liquor in the reactor 1 is adjusted.

In one embodiment of the present application, it is preferable that a stirring device 16 is further provided in the middle of the reactor 1 as shown in fig. 1 for stirring the mixed liquid in the reactor 1. The reactor 1 is also internally provided with a full-spectrum lighting device 17, the full-spectrum lighting device 17 comprises a plurality of lighting lamp grooves which are arranged around the circumference of the stirring device 16, and a full-spectrum lighting lamp can be arranged in each lighting lamp groove to provide illumination for the culture of microorganisms in the reactor 1.

Preferably, as shown in fig. 1, the reactor 1 is further provided with a viewing window 18 to facilitate the operator to observe the conditions inside the reactor 1.

Preferably, as shown in fig. 1, a wall scraper 19 is further provided on the reactor 1, and the wall scraper 19 is adsorbed on the inner side wall of the cylinder of the reactor 1 and the outer side wall of the jacket cylinder to scrape the inner wall of the cylinder of the reactor 1.

In one embodiment of the present application, preferably, the closed microbial cultivation system further comprises a cultivation parameter online monitoring module; the culture parameter on-line monitoring module comprises a plurality of sensors arranged on the reactor 1 and is used for monitoring the pH value, the temperature, the DO value (dissolved oxygen content), the illumination intensity, the turbidity and the chlorophyll fluorescence in the reactor 1.

The culture parameter on-line monitoring module further comprises a liquid level sensor arranged on the material supplementing device 2 and used for detecting the liquid level in the material supplementing device 2.

Preferably, the closed microorganism culture system further comprises a control system 9, and the culture parameter online monitoring module is in communication connection with the control system 9 so as to feed back the monitored data to the control system 9 and analyze each item of received data through the control system 9.

The temperature control device, the full-spectrum lighting device 17, the acid-base adjusting device, the liquid path circulating module 4, the gas path circulating module 5 and the stirring device 16 of the reactor 1 are also respectively in communication connection with the control system 9. Through the analysis of the control system 9 on each item of data, the control system 9 can control the operation of the corresponding device or module, so that the data measured by each sensor conforms to the set value. Taking the pH adjusting device 8 as an example, when the pH detecting sensor detects that the pH of the mixed liquid in the reactor 1 is not within the set range, the control system 9 can control the corresponding alkali liquid feed pump or acid liquid feed pump to feed alkali liquid or acid liquid into the reactor 1, so as to adjust the pH in the reactor 1 to be within the set range.

Therefore, the closed microorganism culture system avoids the interference of mixed bacteria to the maximum extent by arranging the gas path circulation module 5 and the liquid path circulation module 4 with adjustable closing degree, can meet the requirement of sealing property in the culture process of different microorganisms, and is particularly suitable for the production process of certain extreme anaerobic microorganisms, deep sea microorganisms and the like which take extreme environment microorganisms as cell factories and the production process of strictly controlling raw materials or production products to enter the external environment based on the requirements of environmental protection, cost saving, stable product control and the like. In addition, by the design of the parameter on-line monitoring module and the intelligent control system 9, the timely transmission, analysis and feedback of the culture parameters and growth state data and process control are ensured, the parameter optimization of the microbial culture process is facilitated, the possibility of improving the productivity is provided, and the manual operation cost is reduced.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.