阅读说明：本技术 进样系统及其控制方法 (Sample introduction system and control method thereof ) 是由 张必良 辜键洲 于 2021-08-30 设计创作，主要内容包括：本发明公开了一种进样系统及其控制方法,该进样系统包括依次设置的通道转换器、泵体和连接件；通道转换器具有连接口、输出口和至少一个输入口,通道转换器能够通过转换自身状态以使连接口与输出口或任一输入口连通或者断开；泵体与通道转换器的连接口连通；连接件具有第一通道与第一通道连通的至少一第二通道,第一通道与输出口连通,各第二通道均通过管道外接芯片的各流动槽。该进样系统及其控制方法提供的技术方案通过通道转换器和连接件配合,能够省略电磁阀单独控制每一试剂流通通道以控制试剂吸取情况,从而能够减小该进样系统的体积,且具有控制简单、无需进行散热设计、有效避免试剂量浪费,减小误差的效果。(The invention discloses a sample introduction system and a control method thereof, wherein the sample introduction system comprises a channel converter, a pump body and a connecting piece which are arranged in sequence; the channel converter is provided with a connecting port, an output port and at least one input port, and can connect or disconnect the connecting port with the output port or any input port by converting the state of the channel converter; the pump body is communicated with a connecting port of the channel converter; the connecting piece is provided with at least one second channel communicated with the first channel, the first channel is communicated with the output port, and each second channel is externally connected with each flow groove of the chip through a pipeline. The technical scheme provided by the sample introduction system and the control method thereof can omit the situation that each reagent circulation channel is independently controlled by the electromagnetic valve to control the reagent suction through the matching of the channel converter and the connecting piece, thereby reducing the volume of the sample introduction system, and having the effects of simple control, no need of heat dissipation design, effective avoidance of reagent quantity waste and error reduction.)

1. The sampling system is characterized by comprising a channel converter, a pump body and a connecting piece which are arranged in sequence;

the channel converter is provided with a connecting port, an output port and at least one input port, and can connect or disconnect the connecting port with the output port or any input port by converting the state of the channel converter;

the pump body is communicated with a connecting port of the channel converter;

the connecting piece is provided with a first channel and at least one second channel communicated with the first channel, the first channel is communicated with the output port, and each second channel is externally connected with each flow groove of the chip through a pipeline.

2. The sample introduction system according to claim 1, wherein the first channel and each of the second channels are disposed at intervals on a peripheral wall of the connector, and each of the first channel and each of the second channels extends along a side wall of the connector to a central position of the connector and has a distal end coinciding with the central position of the connector.

3. The sample introduction system according to claim 2, wherein a radial area of the first channel and/or the second channel near the overlap is smaller than a radial area near the side wall of the connection piece.

4. The sampling system of claim 1, wherein the connector further comprises a third channel, a solenoid valve and at least one check valve, the third channel is controlled by the solenoid valve to communicate with an external waste liquid collecting device, and each of the second channels is controlled by each of the check valves to communicate with each of the flow channels of the external chip.

5. The sampling system of claim 1, wherein the channel switch comprises a body and a switch movably engaged with the body, the body has the connection port, the output port, and the input port, and the switch is engaged with the body to connect or disconnect the connection port with the output port or any input port.

6. The sample introduction system according to claim 5, wherein the body further has a positioning hole and a first guiding groove, and the connection port is communicated with the positioning hole through the first guiding groove;

the switching piece is rotatably covered on the body and provided with a second diversion trench, and the switching piece is rotated to the two ends of the second diversion trench to be respectively communicated with the positioning hole and the input port, or rotated to the two ends of the second diversion trench to be respectively connected with the positioning hole and the output port.

7. The sampling system of claim 6, wherein the connection port, the output port, and the input ports are all disposed on a side of the body near the switch, and the connection port, the output port, and the input ports are arranged at intervals along a circumferential direction of the body.

8. The sampling system of claim 7, wherein the body and the switching member are both of a solid-of-revolution structure, the positioning hole is disposed on a central axis of the body, a distance from the positioning hole to the input port or the output port is consistent with a length of the second guiding groove, and the switching member rotates around the positioning hole.

9. The sample introduction system according to claim 5, wherein at least two of the channel switch, the pump body and the first channel are provided, and the channel switch, the pump body and the first channel are provided in a one-to-one correspondence.

10. The control method of the sample injection system is characterized by comprising the following steps:

after the input port of the channel converter is communicated with the connecting port, the pump body sucks the reagent, and the reagent is transmitted to the pump body through the input port and the connecting port of the channel converter;

the channel converter converts the self state, the output port of the channel converter is communicated with the connecting port, and the pump body transmits the sucked reagent to the connecting piece through the connecting port and the output port of the channel converter;

the first channel of the connector receives a reagent and flows to the flow channel of the chip through the second channel of the connector.

Technical Field

The invention belongs to the technical field of micro-flow sample introduction, and particularly relates to a sample introduction system and a control method thereof.

Background

At present, in micro-flow sample injection of a sequencer, two modes are mainly adopted, namely 1, 1 pump body, 1 electromagnetic valve for sample injection and 1 electromagnetic valve for sample outlet are matched for each reagent input channel, and different reagents can flow to the same outlet at the outlet and then enter a flow groove; before entering the flowing groove, a three-way electromagnetic valve with 1 inlet and 2 outlets is used for switching to push the reagent to one of 2 flow channels in the flowing groove; the method has the following defects: the scheme of using a plurality of pump bodies has high cost, large volume of the whole system and complex control. 2. 1 single electromagnetic valve needs to be matched with each reagent input channel, and the reagent input channels flow to the same pump body. When a certain reagent needs to be extracted, the corresponding electromagnetic valve is opened, the pump body is used for sucking liquid, and before the reagent enters the flow groove, the three-way electromagnetic valve with 1 inlet and 2 outlets is used for switching to push the reagent to the flow groove. The method has the following defects: the cost is high, and entire system's is bulky, and the integrated level is low, causes the pipeline internal volume big, causes reagent extravagant, and the solenoid valve generates heat greatly, if do not carry out the heat dissipation design, can't be in the open mode for a long time. In addition, in both of the above two methods, an electromagnetic valve is used, when the flow channel is closed, a certain space in the flow channel is cut off to prevent the reagent from flowing, and this action compresses the space in the flow channel, and squeezes the reagent in the flow channel to flow to both sides or one side, depending on the fluid pressure difference at both ends of the electromagnetic valve; when the flow channel is opened, the space of the flow channel becomes larger, the reagents at the two ends of the electromagnetic valve can fill the extra space, the action can cause the shaking of the nearby reagents, and the shaking amount of the reagents at the two ends is also determined by the pressure difference at the two ends. The space change caused by the switching of the electromagnetic valve is called the action volume of the electromagnetic valve, and the action volume of the electromagnetic valve is positioned between the pump body and the flow groove, so that the reagent can move towards an uncertain direction, and the used reagent amount is more. For a normal solenoid valve, the action volume of the solenoid valve is between 40 and 160uL, which has a large error for a micro-flow channel of a sequencer.

Disclosure of Invention

The invention aims to provide a sample introduction system and a control method thereof, which are used for overcoming the defects of large volume, complex control, heat dissipation design, more reagent consumption and larger error of micro-flow sample introduction in the prior art.

The technical scheme is as follows:

a sample introduction system comprises a channel converter, a pump body and a connecting piece which are arranged in sequence;

the channel converter is provided with a connecting port, an output port and at least one input port, and can connect or disconnect the connecting port with the output port or any input port by converting the state of the channel converter;

the pump body is communicated with a connecting port of the channel converter;

the connecting piece is provided with a first channel and at least one second channel communicated with the first channel, the first channel is communicated with the output port, and each second channel is externally connected with each flow groove of the chip through a pipeline.

In one embodiment, the first channel and each second channel are arranged on the peripheral wall of the connecting piece at intervals, and the first channel and each second channel extend to the central position of the connecting piece along the side wall of the connecting piece, and the tail ends of the first channel and each second channel are overlapped with the central position of the connecting piece.

In one embodiment, the radial area of the first channel and/or the second channels near the overlap is smaller than the radial area near the side wall of the connector.

In one embodiment, the connector further comprises a third channel, an electromagnetic valve and at least one-way valve, wherein the third channel is controlled by the electromagnetic valve to be communicated with an external waste liquid collecting device, and each second channel is controlled by each one-way valve to be communicated with each flow groove of an external chip.

In one embodiment, the channel converter comprises a body and a switching piece movably matched with the body, wherein the body is provided with the connecting port, the output port and the input port, and the switching piece is matched with the body to enable the connecting port to be connected with or disconnected from the output port or any input port.

In one embodiment, the body is further provided with a positioning hole and a first diversion trench, and the connecting port is communicated with the positioning hole through the first diversion trench;

the switching piece is rotatably covered on the body and provided with a second diversion trench, and the switching piece is rotated to the two ends of the second diversion trench to be respectively communicated with the positioning hole and the input port, or rotated to the two ends of the second diversion trench to be respectively connected with the positioning hole and the output port.

In one embodiment, the connection port, the output port and the input ports are all arranged on one side of the body close to the switching piece, and the connection port, the output port and the input ports are arranged at intervals along the circumferential direction of the body.

In one embodiment, the body and the switching member are both of a revolving body structure, the positioning hole is formed in a central axis of the body, a distance from the positioning hole to the input port or the output port is consistent with a length of the second diversion trench, and the switching member rotates around the positioning hole.

In one embodiment, the channel converter, the pump body and the first channel are provided with at least two channels, and the channel converter, the pump body and the first channel are provided in a one-to-one correspondence manner.

A control method of a sample introduction system comprises the following steps:

after the input port of the channel converter is communicated with the connecting port, the pump body sucks the reagent, and the reagent is transmitted to the pump body through the input port and the connecting port of the channel converter;

the channel converter converts the self state, the output port of the channel converter is communicated with the connecting port, and the pump body transmits the sucked reagent to the connecting piece through the connecting port and the output port of the channel converter;

the first channel of the connector receives a reagent and flows to the flow channel of the chip through the second channel of the connector.

The technical scheme provided by the invention has the following advantages and effects:

the sampling system can perform micro-flow sampling operation by mutually matching the channel converter, the pump body and the connecting piece, wherein the self state is converted by the channel converter so as to switch different flow paths, so that different input ports can respectively absorb reagents of different reagent containers, and the reagents are shunted to different flow grooves of the chip through second channels of the connecting piece. Therefore, through the cooperation of channel converter and connecting piece, can omit each reagent circulation passageway of solenoid valve independent control and absorb and the reposition of redundant personnel condition with control reagent, and through pump body control self connector and input port or delivery outlet intercommunication, can control the suction and the suction of reagent, form the solvent residue in avoiding the channel converter, thereby can reduce and advance a kind volumetric error, it is less to make the error between actual sample introduction volume and the predetermined sample introduction volume, effectively avoid the reagent volume extravagant, and can reduce the volume of this kind system, and has the characteristics that control is simple, the structure integrated level is high, need not to carry out heat dissipation design.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and, together with the description, serve to explain the principles and effects of the invention.

Unless otherwise specified or defined, the same reference numerals in different figures refer to the same or similar features, and different reference numerals may be used for the same or similar features.

FIG. 1 is a schematic perspective view of a sample injection system according to an embodiment of the present invention;

FIG. 2 is a schematic structural diagram of a connector of the sample injection system of FIG. 1;

FIG. 3 is a schematic cross-sectional view of a connector of the sample injection system of FIG. 2;

FIG. 4 is a schematic structural diagram of a body of the channel switch;

fig. 5 is a schematic structural diagram of a switching element of the channel converter.

Description of reference numerals:

1. a channel converter; 11. a body; 111. a connecting port; 112. an output port; 113. an input port; 114. positioning holes; 115. a first diversion trench; 12. a switching member; 121. a second guiding gutter; 2. a pump body; 3. a connecting member; 31. a first channel; 32. a second channel; 33. a third channel; 4. an electromagnetic valve; 5. a one-way valve;

20. a chip; 21. a flow channel.

Detailed Description

In order to facilitate an understanding of the invention, specific embodiments thereof will be described in more detail below with reference to the accompanying drawings.

Unless specifically stated or otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In the case of combining the technical solutions of the present invention in a realistic scenario, all technical and scientific terms used herein may also have meanings corresponding to the purpose of achieving the technical solutions of the present invention.

As used herein, unless otherwise specified or defined, "first" and "second" … are used merely for name differentiation and do not denote any particular quantity or order.

As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items, unless specified or otherwise defined.

It will be understood that when an element is referred to as being "secured to" another element, it can be directly secured to the other element or intervening elements may also be present; when an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present; when an element is referred to as being "mounted on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "on" another element, it can be directly on the other element or intervening elements may also be present.

The invention provides a sample introduction system, which comprises a channel converter 1, a pump body 2 and a connecting piece 3 which are sequentially arranged as shown in figure 1.

As shown in fig. 1 to 5, the channel switch 1 has a connection port 111, an output port 112, and at least one input port 113, and the channel switch 1 is capable of connecting or disconnecting the connection port 111 with or from the output port 112 or any of the input ports 113 by switching its own state; it should be noted that the input ports 113 of the channel switch 1 may be externally connected with reagent containers, and each input port 113 may correspond to one reagent container, so that the plurality of input ports 113 may correspond to a plurality of reagent containers, respectively, so that the reagents of the corresponding reagent containers are input into the channel switch 1 through the input ports 113, wherein each reagent container may be loaded with various types of reagents, such as one loaded with a reagent stored at normal temperature and the other loaded with a reagent stored at a refrigerated temperature, and the present invention is not limited thereto. It should be noted that one or more output ports 112 may be provided, and are not particularly limited herein.

The pump body 2 is communicated with a connecting port 111 of the channel converter 1; when the input port 113 and the connection port 111 of the channel changer 1 are communicated, the reagent is sucked by the pump body 2, the reagent flows into the pump body 2, then the connection port 111 is communicated with the output port 112, and the reagent sucked by the pump body 2 flows out from the output port 112 of the channel changer 1, it is understood that the pump body 2 is arranged to be communicated with the input port 113 or the output port 112 through the connection port 111 of the channel changer 1 to be able to control the suction and suction of the reagent to prevent the formation of solvent residue in the channel changer 1.

The connector 3 has at least one second channel 32, in which the first channel 31 is communicated with the first channel 31, the first channel 31 is communicated with the output port 112, each second channel 32 is externally connected to each flow groove 21 of the chip 20 through a pipeline, it should be noted that, in the present embodiment, each second channel 32 corresponds to one flow groove 21, so that the reagent can flow into the corresponding flow groove 21 through each second channel 32; of course, in other embodiments, the second channel 32 may correspond to a plurality of flow grooves 21, and is not particularly limited herein. It can be understood that the reagent flowing out from the channel changer 1 flows in through the first channel 31 of the connecting member 3 and flows out to the corresponding flow groove 21 through the corresponding second channel 32, so that different reagents of different reagent containers can flow to different second channels 32 of the connecting member 3 through the channel changer 1 to different input ports 113 and enter different flow grooves 21 of the chip 20. It should be noted that the number of the input ports 113 and the number of the second channels 32 can be set according to actual conditions, and in this embodiment, at least two input ports 113 and at least two second channels 32 are provided to be able to suck and transfer the reagents of at least two reagent containers to different flow channels 21 respectively.

It can be understood that, in the present embodiment, the working principle of the sample injection system with two input ports 113 and two second channels 32 is substantially as follows:

when the reagents of two reagent containers need to be injected, one of the input ports 113 and the connecting port 111 of the channel converter 1 are communicated with each other, the pump body 2 sucks the reagent of one of the reagent containers, the reagent enters the pump body 2 through the input port 113, after the suction is finished, the channel converter 1 converts the self state to communicate the connecting port 111 with the output port 112, and the reagent sequentially enters the first channel 31 and one of the second channels 32 of the connecting part 3 from the pump body 2 and flows out to one of the flow grooves 21 of the chip 20; then the channel switch 1 switches its state to communicate the other input port 113 with the connection port 111, the pump body 2 sucks the reagent of the other reagent container, the reagent enters the pump body 2 through the input port 113, after the suction is completed, the channel switch 1 switches its state to communicate the connection port 111 with the output port 112, and the reagent enters the first channel 31 of the connection member 3 from the pump body 2 in sequence and flows out of the other second channel 32 to the other flow groove 21 of the chip 20.

In summary, the sample injection system can perform micro-flow sample injection operation by the mutual cooperation of the channel converter 1, the pump body 2 and the connector 3, wherein the channel converter 1 converts its own state to switch different flow paths, so that different input ports 113 can respectively suck reagents of different reagent containers, and the reagents are distributed to different flow channels 21 of the chip 20 through the second channels 32 of the connector 3. Therefore, through the cooperation of channel converter 1 and connecting piece 3, can omit each reagent circulation passageway of solenoid valve independent control and absorb and the reposition of redundant personnel condition with control reagent, and set up connector 111 and input port 113 or delivery outlet 112 intercommunication that the pump body 2 passes through channel converter 1, can control the suction and the suction of reagent, form the solvent residue in avoiding channel converter 1, thereby can reduce and advance a kind bulk error, it is less to make the error between actual sample introduction volume and the predetermined sample introduction volume, effectively avoid the reagent volume extravagant, and can reduce this sampling system 100's volume, and has simple control, the structure integrated level is high, need not to carry out the characteristics of heat dissipation design.

In some embodiments, the first channel 31 and each second channel 32 are disposed at intervals on the peripheral wall of the connecting part 3, and the first channel 31 and each second channel 32 both extend along the side wall of the connecting part 3 to the central position of the connecting part 3 and have ends coinciding with the central position of the connecting part 3, so that the connecting part 3 can integrate the first channel 31 and the plurality of second channels 32, the structure compactness is improved, the distance for flowing the reagent from the first channel 31 to the second channels 32 can be reduced, and the sample injection efficiency is improved.

In some embodiments, the radial area of the first channel 31 and/or each second channel 32 near the coincidence is smaller than the radial area near the side wall of the connection 3. It can be understood that, in the present embodiment, the radial areas of the first channel 31 and each second channel 32 near the overlapping portion are smaller than the radial areas near the side wall of the connecting member 3, wherein one end of the first channel 31 near the side wall of the connecting member 3 is connected to the output port 112 of the channel converter 1 through a pipe, a larger radial area can facilitate connection with the pipe, and similarly, one end of the second channel 32 near the side wall of the connecting member 3 is connected to the flow groove 21 of the chip 20 through a pipe, a larger radial area can facilitate connection with the pipe, and a smaller radial area of the first channel 31 and each second channel 32 near the overlapping portion can better integrate the first channel 31 and each second channel 32 in the connecting member 3, thereby improving the space utilization.

In some embodiments, the connector 3 further has a third channel 33 and a solenoid valve 4, and the third channel 33 is controlled to communicate with an external waste liquid collecting device through the solenoid valve 4. Specifically, the solenoid valve 4 is disposed at a position of the pipe between the third channel 33 and the waste liquid collecting device. It should be noted that the input port 113 can be connected to a container containing a cleaning reagent, the pump body 2 sucks the cleaning reagent, the cleaning reagent flows through the channel switch 1 and then enters the pump body 2, and then flows through the channel switch 1 and enters other reagent containers, and the cleaning reagent can clean each reagent container and pipeline in the process of repeatedly flowing through different reagent containers, and then flows into the waste liquid collecting device for collection after cleaning the connecting member 3. Similarly, as shown in fig. 1, the electromagnetic valve 4 may also be disposed in a pipeline outside the outlet of the flow groove 21 of the external chip 20, so that the cleaning reagent can clean each reagent container and pipeline, and then sequentially clean the connection member 3 and the flow groove 21 of the chip 20 and then flow through the waste liquid collecting device.

In some embodiments, as shown in fig. 1, the connector 3 further comprises at least one-way valve 5, and each second channel 32 is controlled to communicate with each flow groove 21 of the external chip 20 through each one-way valve 5. It will be appreciated that the one-way valve 5 is adapted to prevent the reagent from flowing backwards, i.e. from flowing backwards from the chip 20 to the second channel 32, thereby preventing the reagent from moving in an undefined direction, effectively preventing the reagent from being wasted and effectively preventing errors in the flow path.

In some embodiments, as shown in fig. 4 and 5, the channel converter 1 includes a body 11 and a switch 12 movably engaged with the body 11, the body 11 has a connection port 111, an output port 112, and an input port 113, and the switch 12 is engaged with the body 11 to connect or disconnect the connection port 111 with or from the output port 112 or any one of the input ports 113. It can be understood that the switching member 12 is movable relative to the body 11 to switch the flow path through the switching member 12, so that the connection port 111 can be sequentially communicated with the output ports 112 or the input ports 113 in a predetermined sequence, and the reagents corresponding to the input ports 113 can be sequentially sucked into the corresponding flow channels 21 of the chip 20.

In some embodiments, as shown in fig. 4 and 5, the body 11 further has a positioning hole 114 and a first guide groove 115, and the connection port 111 and the positioning hole 114 are communicated through the first guide groove 115. The switch 12 is rotatably covered on the main body 11, and specifically, the switch 12 is rotatably covered on the upper side of the main body 11 or on the lower side of the main body 11, which is not particularly limited herein. The switching member 12 has a second guiding groove 121, and the switching member 12 rotates to the two ends of the second guiding groove 121 to respectively communicate with the positioning hole 114 and the input port 113, or rotates to the two ends of the second guiding groove 121 to respectively connect with the positioning hole 114 and the output port 112. It can be understood that, the two ends of the second guiding groove 121 are respectively communicated with the positioning hole 114 and the input port 113 by the rotation of the switching member 12, at this time, the input port 113, the second guiding groove 121, the positioning hole 114, the first guiding groove 115 and the connection port 111 are sequentially communicated, the pump body 2 can suck the reagent of the reagent container, the reagent enters the pump body 2 through the input port 113, after the suction is completed, the two ends of the second guiding groove 121 are respectively connected with the positioning hole 114 and the output port 112 by the rotation of the switching member 12, at this time, the connection port 111, the first guiding groove 115, the positioning hole 114, the second guiding groove 121 and the output port 112 are sequentially communicated, and the reagent enters the connection member 3 from the pump body 2 and then flows out to the flow groove 21 of the chip 20; the sequential circulation allows the reagents of the respective reagent containers to be sucked into the respective flow grooves 21 of the chip 20. Therefore, the reagent of each reagent container can be sucked by rotating the switching piece 12, the operation is simple and convenient, and the reagent can be prevented from moving towards an uncertain direction.

In some embodiments, as shown in fig. 4, the connection port 111, the output port 112, and each input port 113 are opened on a side of the body 11 close to the switch 12, and the connection port 111, the output port 112, and each input port 113 are arranged at intervals along the circumferential direction of the body 11. It can be understood that by integrating the connection port 111, the output port 112, and the input ports 113 in one body 11, the second guide groove 121 of the switching member 12 can be switched and communicated between the input ports 113 and the output port 112 quickly and accurately, thereby improving the working efficiency.

In some embodiments, as shown in fig. 4 and 5, the body 11 and the switching member 12 are both of a solid structure, the positioning hole 114 is disposed on the central axis of the body 11, the switching member 12 rotates around the positioning hole 114, the distance from the positioning hole 114 to the input port 113 or the output port 112 is the same as the length of the second guiding groove 121, and the second guiding groove 121 rotates around the positioning hole 114. It can be understood that when the switching member 12 rotates around the positioning hole 114, the second guiding groove 121 can be rotated and switched around the positioning hole 114 by setting the switching member 12 to rotate around the positioning hole 114, and the distance from the positioning hole 114 to the input port 113 or the output port 112 is consistent with the length of the second guiding groove 121, so that the second guiding groove 121 can be accurately rotated to be communicated with the input port 113 or the output port 112. Specifically, the switch 12 may be a rotor valve core, and the body 11 may be a stator valve disk, wherein the lower portion of the stator valve disk and the rotor valve core are attached to each other. Of course, in other embodiments, the switch 12 and the body 11 may be other devices capable of rotating relatively, and are not limited herein.

In some embodiments, a sealing surface structure is formed between switch 12 and body 11 to prevent a gap from being formed between switch 12 and body 11 during rotation of switch 12 relative to body 11, resulting in reagent extravasation. In addition, the radial area of the first guide groove 115 is smaller than the radial areas of the positioning hole 114 and the connection port 111, and the radial area of the second guide groove 121 is smaller than the radial areas of the positioning hole 114, the input port 113 and the output port 112, so that the positioning hole 114, the connection port 111, the input port 113 and the output port 112 cooperate to form a buffer, so that the flow rate of the reagent stably flows out to the flow groove 21 of the chip 20.

In some embodiments, as shown in fig. 1, at least two of the channel changer 1, the pump body 2 and the first channel 31 are provided, and the channel changer 1, the pump body 2 and the first channel 31 are all correspondingly provided. It should be noted that each channel converter 1 may correspond to a plurality of reagent containers, a plurality of reagent containers of each channel converter 1 form a reagent container group, a plurality of channel converters 1 correspond to a plurality of reagent container groups, and a plurality of reagent suction devices may be integrated in one sample injection system, so that the structural compactness may be further improved, the volume may be further reduced, and the reagent containers may be increased or decreased according to the number of reagents to be injected.

In some embodiments, the connecting member 3 may have one second channel 32, two second channels 32, three second channels 32, etc., and is not particularly limited herein.

In some embodiments, the sample injection system 100 further includes a mounting seat, and a rotation driving device and a lifting driving device disposed in the mounting seat, the pump body 2 includes an injection pump, the rotation driving device is connected to the switching member 12, the lifting driving device is connected to a piston end of the injection pump, the rotation driving device automatically drives the switching member 12 to rotate relative to the body 11, and the lifting driving device automatically drives the piston of the injection pump to move up and down to suck and discharge the reagent, so that automatic sample injection operation can be performed through cooperation of the rotation driving device and the lifting driving device, and automatic operation of the sample injection system 100 can be improved.

In some embodiments, the channel changer 1 and the pump body 2 are coaxially disposed on one side of the mounting seat, and the channel changer 1 is disposed above the pump body 2, so as to improve the compactness of the structure, and make the position of the pump body 2 and the channel changer 1 suitable. Specifically, the mounting base is further provided with a mounting part, the tail end of a piston of the pump body 2 is connected with a lifting driving device through the mounting part, and the mounting part is bent to form an L-shaped structure, so that the lifting driving device can smoothly drive the pump bodies 2 which are not coaxial to perform lifting motion, and the position flexibility of the structure is improved.

The invention also provides a control method of the sample injection system, which comprises the following steps:

after the input port 113 of the channel converter 1 is communicated with the connection port 111, the pump body 2 sucks the reagent, and the reagent is transmitted to the pump body 2 through the input port 113 and the connection port 111 of the channel converter 1;

the channel converter 1 converts the self state, the output port 112 of the channel converter 1 is communicated with the connecting port 111, and the pump body 2 transmits the sucked reagent to the connecting piece 3 through the connecting port 111 and the output port 112 of the channel converter 1;

the first channel 31 of the connector 3 receives the reagent and flows to the flow channel 21 of the chip 20 through the second channel 32 of the connector 3.

When the other input port 113 needs to be replaced to aspirate the reagent, the channel changer 1 may change its state so that the other input port 113 communicates with the connection port 111, and the above steps may be repeated.

In summary, the sample injection system control method can perform micro-flow sample injection operation by the mutual cooperation of the channel converter 1, the pump body 2 and the connector 3, wherein the channel converter 1 converts its own state to switch different flow paths, so that different input ports 113 can respectively suck reagents of different reagent containers, and the reagents are distributed to different flow channels 21 of the chip 20 through the second channels 32 of the connector 3. Therefore, through the cooperation of channel converter 1 and connecting piece 3, can omit each reagent circulation passageway of solenoid valve independent control and absorb and the reposition of redundant personnel condition with control reagent, and set up connector 111 and input port 113 or delivery outlet 112 intercommunication that the pump body 2 passes through channel converter 1, can control the suction and the suction of reagent, form the solvent residue in avoiding channel converter 1, thereby can reduce and advance a kind bulk error, it is less to make the error between actual sample introduction volume and the predetermined sample introduction volume, effectively avoid the reagent volume extravagant, and can reduce this sampling system 100's volume, and has simple control, the structure integrated level is high, need not to carry out the characteristics of heat dissipation design.

When the drawing description is quoted, the new characteristics are explained; in order to avoid that repeated reference to the drawings results in an insufficiently concise description, the drawings are not referred to one by one in the case of clear description of the already described features.

The above embodiments are provided to illustrate, reproduce and deduce the technical solutions of the present invention, and to fully describe the technical solutions, the objects and the effects of the present invention, so as to make the public more thoroughly and comprehensively understand the disclosure of the present invention, and not to limit the protection scope of the present invention.

The above examples are not intended to be exhaustive of the invention and there may be many other embodiments not listed. Any alterations and modifications without departing from the spirit of the invention are within the scope of the invention.