Novel triterpene derivatives as HIV inhibitors

文档序号：1909309 发布日期：2021-11-30 浏览：14次中文

阅读说明：本技术 作为hiv抑制剂的新型三萜衍生物 (Novel triterpene derivatives as HIV inhibitors ) 是由 P.R.班迪 R.R.库拉 P.R.阿杜拉 B.R.卡西雷迪于 2020-02-11 设计创作，主要内容包括：本发明涉及式(I)的新型三萜衍生物；及其药学上可接受的盐,其中R-(1)、R-(2)、R-(3)、R-(4)和环如本文所定义。本发明还涉及用于治疗性治疗病毒性疾病,特别是HIV介导的疾病的新型三萜衍生物、相关化合物和药物组合物。(The present invention relates to novel triterpene derivatives of formula (I); and pharmaceutically acceptable salts thereof, wherein R 1 、R 2 、R 3 、R 4 And rings are as defined herein. The invention also relates to novel triterpene derivatives, related compounds and pharmaceutical compositions useful in the therapeutic treatment of viral diseases, particularly HIV mediated diseases.)

1. A compound of formula (I):

wherein the content of the first and second substances,

R₁is selected from

R₂Is selected from C₁-C₆Alkyl, optionally substituted C₂-C₆Alkenyl or optionally substituted C₃-C₈A cycloalkyl group; wherein said optional substituent is C₁-C₆An alkyl group;

R₃is hydrogen or C₁-C₆An alkyl group;

R₄selected from optionally substituted C₁-C₆Alkyl OR-C (O) OR_a(ii) a Wherein the optional substituents are selected from the group consisting of halogen, hydroxy, alkoxy, -OC (O) CH₂Alkoxy, -OSi (R)_a)₃、-N(R_a)(R_b)、-S(R_a) or-O-CH₂-P(O)(OR_a)₂；

Ring (C)Is composed of

R_aIs hydrogen or C₁-C₆An alkyl group; and is

R_bSelected from hydrogen, alkyl, -C (O) alkoxy or-S (O)₂Alkyl, or

A pharmaceutically acceptable salt thereof, a pharmaceutically acceptable stereoisomer, a prodrug, or a combination thereof.

2. The compound according to claim 1, which is a compound of formula (IA):

wherein the content of the first and second substances,

R₂is composed of

R₃Is hydrogen or C₁-C₆An alkyl group;

Ring (C)Is composed of

R_aIs hydrogen or C₁-C₆An alkyl group; and is

R_bSelected from hydrogen, alkyl, -C (O) alkoxy or-S (O)₂Alkyl, or

A pharmaceutically acceptable salt thereof, a pharmaceutically acceptable stereoisomer, a prodrug, or a combination thereof.

3. A compound according to claim 1, which is a compound of formula (IB):

wherein the content of the first and second substances,

R₃is hydrogen or C₁-C₆An alkyl group;

R_aIs hydrogen or C₁-C₆An alkyl group; and is

R_bSelected from hydrogen, alkyl, -C (O) alkoxy or-S (O)₂Alkyl, or

A pharmaceutically acceptable salt thereof, a pharmaceutically acceptable stereoisomer, a prodrug, or a combination thereof.

4. A compound selected from:

(1R,3S) -3- (((((1R, 3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- ((2- (1, 1-thiomorpholino) -2-methylpropyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid,

(1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((S) -2- (1, 1-thiomorpholino) propyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid,

(1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((R) -2- (1, 1-thiomorpholino) -3-methylbutyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid,

(1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((R) -3- ((tert-butyldimethylsilyl) oxy) -2- (1, 1-thiomorpholino) propyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid,

(1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((R) -2- (1, 1-thiomorpholino) -3-hydroxypropyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid,

(1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((S) -2- (1, 1-thiomorpholino) -3-methylbutyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid,

(1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3R) -2- (1, 1-thiomorpholino) -3-hydroxybutyl) amino) -5a,5b,8,8,11 a-pentakisMethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid,

(1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((S) -2- (1, 1-thiomorpholino) -3-hydroxypropyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid,

(1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((S) -2- (1, 1-thiomorpholino) -3-fluoropropyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid,

(1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((R) -2- (1, 1-thiomorpholino) -3-fluoropropyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid,

(1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((S) -2- (4- (isopropylsulfonyl) piperazin-1-yl) propyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid,

(1R,3S)-3-((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3S) -2- (1, 1-thiomorpholino) -3-fluorobutyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-hydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid,

(1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2S,3S) -2- (1, 1-thiomorpholino) -3-hydroxybutyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid,

(1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3R) -3-hydroxy-2- (4- (isopropylsulfonyl) piperazin-1-yl) butyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid,

(1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3R) -2- (1, 1-thiomorpholino) -3-hydroxybutyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosahydro-1H-cyclopenta [ a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid,

(1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((R) -2-carboxy-2- (1, 1-thiomorpholino) ethyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid,

(1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3R) -2- (1, 1-thiomorpholino) -3- (2- (2-methoxyethoxy) acetoxy) butyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid,

(1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((12R,13R) -13- (1, 1-thiomorpholino) -12-methyl-2, 5,8, 11-tetraoxatetradecan-14-yl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid,

(1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3R) -2- (1, 1-thiomorpholino) -3- (2- (2-methoxyethoxy) acetoxy) butyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid,

4- (((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3R) -2- (1, 1-thiomorpholino) -3-hydroxybutyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) oxy) -2, 2-dimethyl-4-oxobutanoic acid,

(1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((15R,16R) -16- (1, 1-thiomorpholino) -15-methyl-13-oxo-2, 5,8,11,14-pentaoxaheptadecan-17-yl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-1H-cyclopenta [ a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid, and

(1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((12R,13R) -13- (1, 1-thiomorpholino) -12-methyl-2, 5,8, 11-tetraoxatetradecan-14-yl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosahydro-1H-cyclopenta [ a-a ] o]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid, or

A pharmaceutically acceptable salt thereof, a pharmaceutically acceptable stereoisomer, a prodrug, or a combination thereof.

5. A pharmaceutical composition comprising a compound according to any one of claims 1-4 and at least one pharmaceutically acceptable excipient.

6. The pharmaceutical composition of claim 5, wherein the pharmaceutically acceptable excipient is a carrier or diluent.

7. A method of preventing, ameliorating or treating a virus-mediated disease, disorder or syndrome in a subject in need thereof comprising administering to the subject a therapeutically effective amount of a compound according to any one of claims 1-4.

8. The method of claim 7, wherein the virus-mediated disease, disorder or syndrome is an HIV infection, HBV infection, HCV infection, retroviral infection genetically related to AIDS, respiratory disorder (including Adult Respiratory Distress Syndrome (ARDS)), inflammatory disease or a combination thereof.

9.A method of treating HIV in a subject in need thereof, comprising administering to the subject a therapeutically effective amount of a compound according to any one of claims 1-4.

10. A method for preventing, ameliorating or treating a virus-mediated disease, disorder or syndrome in a subject in need thereof comprising administering to the subject a pharmaceutical composition according to claims 5-6 comprising a therapeutically effective amount of the compound.

11. The method of claim 10, wherein the virus-mediated disease, disorder or syndrome is HIV infection, HBV infection, HCV infection, retroviral infection genetically related to AIDS, respiratory disorders including Adult Respiratory Distress Syndrome (ARDS), inflammatory diseases or a combination thereof.

Technical Field

The present invention relates to novel triterpene derivatives and related compounds, compositions useful in the treatment of viral diseases, particularly HIV mediated diseases.

Background

Human Immunodeficiency Virus (HIV) has been identified as the causative agent of acquired immunodeficiency syndrome (AIDS) for over 20 years (Science 1983,220, 868-871; N.Eng.J.Med.1984,311, 1292-1297). AIDS is characterized by destruction of the immune system, particularly CD4+ T cells. HIV is a retrovirus, and the HIV life cycle includes several key steps, starting with attachment of the virus to the host cell membrane and ending with release of the progeny virus from the cell.

Betulinic acid (betulinic acid), a natural compound isolated from Syzygium clavifolium and several other plant species, was found to have anti-HIV activity. Several groups have performed chemical modifications in an attempt to identify effective anti-HIV agents by making semisynthetic analogues of betulinic acid, which led to the discovery of bevirimat as a compound with a novel mechanism of action (j.nat. prod.1994,57 (2): 243-7; j.med. chem.1996,39(5), 1016). Further studies have shown that Beevirlima works by disrupting Gag treatment (Proc. Natl. Acad. Sci. USA 2003,100 (23): 13555-60; Antericrob. Agents. Chemother.2001,45 (4); 1225-30; J. Virol.2004,78 (2): 922-9; J. biol. chem.2005,280 (51): 42149-55; J. Virol.2006,80 (12): 5716-22) and is the first (first-in-class) maturation inhibitor with potent activity against HIV-1. Phase two clinical trials were conducted on blephar equine, and not all patients given it had a robust reduction in viral load despite clinically optimal plasma concentrations. Non-responsive patients are reported to have more frequent baseline Gag polymorphisms near the capsid SP-1 cleavage site than responders. (HIV gag polymorphism reagents response to viromat. XVII International HIV drug resistance work shop, 6.10.14.2008, Sitges, Spain).

Encouraged by these developments, medicinal chemists began to explore the therapeutic activity of betulinic acid derivatives and related compounds. For example, WO2017/149518 discloses C-3 novel triterpenes with C-17 amine derivatives as hiv inhibitors; WO2014/105926 discloses novel betulinic acid proline derivatives as HIV inhibitors; WO2014/130810 describes the preparation of C3 alkyl and alkenyl modified betulinic acid derivatives for the treatment of HIV; WO2014/123889 describes the preparation of triterpene derivatives useful as HIV maturation inhibitors; WO2013/160810 discloses novel betulinic acid derivatives as HIV inhibitors; WO2013/169578 describes triterpenoid C-17 bicyclic amines with HIV maturation inhibitory activity and their preparation; WO2013/123019 describes C-3 cycloalkenyl triterpenes with HIV maturation inhibitory activity; WO2013/043778 describes novel betulinic acid derivatives with antiviral activity; WO2013/035943 describes betulinic acid and derivatives thereof with anti-aging activity; WO2012/106190 describes the preparation of C17 and C3 modified triterpenoids with HIV maturation inhibitory activity; CN102399254 describes a new pentacyclic triterpene derivative, its preparation method and application for preventing and treating diabetes, cardiovascular disease, cerebrovascular disease and tumor. WO2011/007230 describes the preparation of lupeol type triterpene derivatives as antiviral agents; WO2010/032123 describes the preparation of triterpenoids for pharmaceutical use; journal of Medicinal Chemistry (2010),53(1), 178-: potential impact on diabetes; WO2009/100532 describes the preparation of 17 β -lupane derivatives for the treatment of HIV infection; CN 101367861 describes a preparation method and application of 2-hydroxy-3-deoxy-pentacyclic triterpene compounds and derivatives thereof; WO2008/138200 describes the preparation of lupane derivatives as NMDA and MC receptor antagonists exhibiting neuroprotective and memory enhancing activity; WO2008/127364 describes the preparation of betulinic acid derivatives for use in antiviral and anticancer pharmaceutical compositions.

Some additional references disclose betulinic acid related compounds. For example, WO2006/053255 describes the preparation of betulin derivatives in pharmaceutical compositions for inhibiting the transmission of viral infections; WO2004/089357 describes antifungal formulations of triterpenes and essential oils; bioorganic & Medicinal Chemistry Letters (2003),13(20), 3549-; russian Journal of biological Chemistry (2003),29(6),594-600 describes the synthesis and antiviral activity of the uridine and carbamates of betulinic acid and its derivatives; journal of Medicinal Chemistry (1996),39(5),1056-68 describes betulinic acid derivatives: a novel type 1 specific inhibitor of human immunodeficiency virus with a novel mode of action; oxidative decarboxylation of acetylbetulinic acid is described by oxidative Communications (1987),10(3-4), 305-12.

In view of the fact that AIDS is at a worldwide epidemic level, there is an urgent need for new effective drugs for treating HIV-infected patients, HIV-mediated disease conditions and/or disorders by discovering new compounds with new structures and/or mechanisms of action.

Disclosure of Invention

The invention relates to compounds of formula (I)

Wherein the content of the first and second substances,

R₁is selected from

R₂Is selected from C₁-C₆Alkyl, optionally substituted C₂-C₆Alkenyl or optionally substituted C₃-C₈A cycloalkyl group; wherein said optional substituent is C₁-C₆An alkyl group;

R₃is hydrogen or C₁-C₆An alkyl group;

Ring (C)Is composed of

R_aIs hydrogen or C₁-C₆An alkyl group; and is

R_bSelected from hydrogen, alkyl, -C (O) alkoxy or-S (O)₂Alkyl, or

A pharmaceutically acceptable salt thereof, a pharmaceutically acceptable stereoisomer, a prodrug, or a combination thereof.

According to one embodiment, provided herein is a compound of formula (I), wherein R is₁Is composed of

According to another embodiment, provided herein is a compound of formula (I), wherein R is₁Is composed of

According to yet another embodiment, provided herein is a compound of formula (I), wherein R is₂Is isopropenyl (isopropenylene).

According to yet another embodiment, provided herein is a compound of formula (I), wherein R is₂Is methylcyclopropyl.

According to yet another embodiment, provided herein is a compound of formula (I), wherein R is₃Is hydrogen.

According to yet another embodimentProvided herein is a compound of formula (I), wherein R is₃Is methyl.

According to yet another embodiment, provided herein is a compound of formula (I), wherein R is₄Is methyl, isopropyl, -C (O) OH,Hydroxyethyl, hydroxymethyl, fluoromethyl, fluoroethyl,

According to yet another embodiment, provided herein is a compound of formula (I), wherein the ringIs composed of

Accordingly, a further aspect of the present invention provides a compound of formula (IA):

wherein the content of the first and second substances,

R₂、R₃、R₄and ringThe same as defined in formula (I); or

A pharmaceutically acceptable salt thereof, a pharmaceutically acceptable stereoisomer, a prodrug, or a combination thereof.

According to one embodiment, provided herein is a compound of formula (IA), wherein R is₂Is an isopropenyl group.

According to another embodiment, provided herein is a compound of formula (IA), wherein R is₂Is methylcyclopropyl.

According to a further embodiment there is provided a compound of formula (IA), wherein R₃Is hydrogen.

According to a further embodiment there is provided a compound of formula (IA), wherein R₃Is methyl.

According to yet another embodiment, provided herein is a compound of formula (IA), wherein R is₄Is methyl, isopropyl, -C (O) OH,Hydroxyethyl, hydroxymethyl, fluoromethyl, fluoroethyl,

According to yet another embodiment, provided herein is a compound of formula (IA), wherein the ringIs composed of

Accordingly, a further aspect of the invention provides a compound of formula (IB):

wherein the content of the first and second substances,

R₂、R₃and R₄The same as defined in formula (I); or

A pharmaceutically acceptable salt thereof, a pharmaceutically acceptable stereoisomer, a prodrug, or a combination thereof.

According to one embodiment, provided herein is a compound of formula (IB), wherein R is₂Is an isopropenyl group.

According to another embodiment, provided herein are compounds of formula (IB), wherein R is₂Is methylcyclopropyl.

According to yet another embodiment, there are provided compounds of formula (IB), wherein R₃Is hydrogen.

According to yet another embodiment, there are provided compounds of formula (IB), wherein R₃Is methyl.

According to yet another embodiment, provided herein is a compound of formula (IB), wherein R is₄Is methyl, isopropyl, -C (O) OH,Hydroxyethyl, hydroxymethyl, fluoromethyl, fluoroethyl,

The following are representative compounds which are for illustrative purposes only and are not intended to limit the scope of the invention (nomenclature has been generated from ChemBioDraw Ultra 13.0 version):

(1R,3S) -3- (((((1R, 3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- ((2- (1, 1-thiomorpholino) -2-methylpropyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopentadien-eAnd [ a ]]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid,

(1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((S) -2- (1, 1-thiomorpholino) -3-methylbutan-eYl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] s]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid,

(1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3R) -2- (1, 1-thiomorpholino) -3-hydroxybutyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid,

(1R,3S)-3-((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((S) -2- (4- (isopropylsulfonyl) piperazin-1-yl) propyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid,

(1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3S) -2- (1, 1-thiomorpholino) -3-fluorobutyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid,

(1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3R) -2- (1, 1-thiomorpholino) -3- (2- (2-methoxyethoxy) acetoxy) butyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid,

(1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((15R,16R) -16- (1, 1-thiomorpholino) -15-methyl-13-oxo-2, 5,8,11, 14-pentaoxaheptadecan-17-yl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-hydro-1H-cyclopenta [ a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid,

(1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((12R,13R) -13- (1, 1-thiomorpholino) -12-methyl-2, 5,8, 11-tetraoxatetradecan-14-yl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosahydro-1H-cyclopenta [ a-a ] o]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid, or

Pharmaceutically acceptable salts and prodrugs of the compounds are also contemplated.

The present invention also provides pharmaceutical compositions comprising at least one compound described herein and at least one pharmaceutically acceptable excipient (e.g., a pharmaceutically acceptable vehicle or diluent). In particular, the pharmaceutical composition comprises a therapeutically effective amount of at least one compound described herein. The compounds present in the compositions may be associated with a pharmaceutically acceptable excipient (e.g., carrier or diluent), or may be diluted by, or enclosed within, a carrier, which may be in the form of a capsule, sachet, or other container.

The compounds and pharmaceutical compositions described herein are useful for treating diseases, conditions, and/or disorders mediated by viral infections.

The present invention further provides a method of treating a disease, condition and/or disorder mediated by a viral infection in a subject in need thereof by administering to the subject a therapeutically effective amount of one or more compounds described herein to cure the infection, particularly in the form of a pharmaceutical composition.

Also provided herein are methods for preparing the compounds described herein.

The present invention provides a method for preventing, ameliorating or treating an HIV-mediated disease, disorder or syndrome in a subject in need thereof, the method comprising administering to the subject a therapeutically effective amount of a compound of the present invention. The invention further provides a method wherein the HIV mediated disease, disorder or syndrome is, for example, AIDS related syndrome or a syndrome characterized by symptoms such as persistent generalized lymphadenopathy, fever, weight loss, or retroviral infections genetically related to AIDS.

The anti-HIV inhibitory potential of the compounds of the invention may be demonstrated by any one or more of the methodologies known in the art, for example, by using the assays described in Mossman T, Decumber 1983, Journal of immunological methods,65(1-2),55-63 and SPC Cole, cancer therapy and Pharmacology,1986,17, 259-263.

Detailed Description

The present invention provides novel triterpene derivatives and related compounds useful as antiviral agents, particularly anti-HIV compounds, and methods of synthesizing these compounds and pharmaceutically acceptable salts thereof, along with pharmaceutically acceptable carriers, excipients, or diluents, which are useful for treating diseases, conditions, and/or disorders mediated by viral infection.

The following definitions apply to the terms used herein:

the term "alkyl" refers to a straight or branched hydrocarbon chain group consisting of only carbon and hydrogen atoms, which is free of unsaturation, has one to eight carbon atoms, and which is attached to the rest of the molecule by a single bond, such as methyl, ethyl, n-propyl, 1-methylethyl (isopropyl), n-butyl, n-pentyl, and 1, 1-dimethylethyl (tert-butyl).

The term "alkenyl" refers to a hydrocarbon chain that may be straight or branched and contains the indicated number of carbon atomsAnd having at least one-C ═ C-, e.g. C₂-C₆Alkenyl groups may have 2 to 6 (inclusive) -C ═ C-atoms therein. C₂-C₆Examples of alkenyl groups include, but are not limited to, ethylene, prop-1-ene, but-2-ene, pent-1-ene, pent-2-ene, hex-1-ene, hex-2-ene, and the like.

The term "alkoxy" refers to a straight or branched hydrocarbon chain consisting of carbon atoms and hydrogen atoms with an oxygen group, which contains saturated or unsaturated bonds, has one to eight carbon atoms, and which is connected to the rest of the molecule via an oxygen atom by a single bond. One hydrogen atom in the alkoxy group may be further substituted with one or more alkoxy groups to further increase the length of the alkoxy chain. Such as methoxy, ethoxy, isopropoxy, n-propoxy, tert-butoxy, 1-methylethoxy (isopropoxy), n-butoxy, n-pentoxy, 1-dimethylethoxy (tert-butoxy),

The term "cycloalkyl" denotes a non-aromatic mono-or polycyclic ring system having from 3 to about 12 carbon atoms, such as, but not limited to, cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. Examples of polycyclic cycloalkyl groups include, but are not limited to, perhydronaphthyl, adamantyl and norbornyl groups, bridged ring groups and spiro bicyclic groups, such as spiro (4,4) non-2-yl.

The term "halogen" or "halo" includes fluorine, chlorine, bromine or iodine.

The term "hydroxy" refers to-OH.

The term "optionally substituted" means that one or more of the hydrogen groups in a given structure are replaced with a group having the substituents specified below, including but not limited to: hydroxyl, halogen, carboxyl, Cyano (CN), nitro, oxo (═ O), thio (═ S), alkyl, methylsulfonyl, haloalkyl, alkoxy, alkenyl, aryl, aralkyl, cycloalkyl, cycloalkylalkyl, amino, -c (O) O-alkyl, heterocyclyl, heteroaryl, heterocyclylalkyl, heteroarylalkyl, alkylthio, arylthio, aryloxy, aminocarbonyl, alkoxycarbonyl, alkylamino, arylamino, acyl, carboxylic acid, sulfonic acid, sulfonyl, phosphonic acid, and aliphatic. It is understood that the substituent may be further substituted.

The term "prodrug" denotes a derivative of a compound which, when administered to a warm-blooded animal such as man, is converted into the compound (drug). The enzymatic and/or chemical hydrolytic cleavage of the compounds of the invention takes place in this way: the proven drug form (parent carboxylic acid drug) is released and the moiety or moieties remain non-toxic or metabolized, resulting in a non-toxic metabolite. For example, the carboxylic acid group can be esterified, for example, with a methyl or ethyl group to form an ester. When the ester is administered to a subject, the ester is enzymatically or non-enzymatically, reductively, oxidatively, or hydrolytically cleaved to expose the anionic group. The anionic groups may be esterified with moieties (e.g., acyloxymethyl esters), which are cleaved to reveal intermediate compounds that are subsequently decomposed to yield the active compounds. Discussions of prodrug use are provided by t.higuchi and w.stella, "Pro-drugs as Novel Delivery Systems," the a.c.s.symposium series, volume 14, and in Bioreversible Carriers in Drug Delivery, ed.edward b.roche, American Pharmaceutical Association and Pergamon Press, 1987.

The term "treating" of a condition, disease, disorder or condition includes:

(1) preventing or delaying the onset of clinical symptoms of a state, disease, disorder, or condition that develops in a subject who may have or be predisposed to having the state, disease, disorder, or condition, but who has not yet experienced or exhibited clinical or subclinical symptoms of the state, disease, disorder, or condition;

(2) inhibiting a condition, disease, disorder or condition, i.e., arresting or reducing the development of a condition, disease, disorder or condition, or at least one clinical or subclinical symptom thereof; or

(3) Alleviating the condition, disease, disorder, or condition, i.e., causing regression of at least one of the condition, disease, disorder, or condition, or clinical or subclinical symptoms thereof.

The benefit to the subject receiving the treatment is statistically significant or at least perceptible to the subject or to a physician.

The term "subject" includes mammals (particularly humans) and other animals, such as domestic animals (e.g., domestic pets, including cats and dogs) and non-domestic animals (e.g., wild animals).

A "therapeutically effective amount" refers to an amount of a compound that is sufficient to effect such treatment when administered to a subject for the treatment of a condition, disease, disorder, or condition. A "therapeutically effective amount" will vary depending on the compound, state, disease, disorder or condition and its severity and the age, weight, physical condition and responsiveness of the subject being treated.

The compounds of the present invention may form salts. Non-limiting examples of pharmaceutically acceptable salts forming part of the invention include salts derived from inorganic bases, salts of organic bases, salts of chiral bases, salts of natural amino acids, and salts of non-natural amino acids. Certain compounds of the present invention can exist in stereoisomeric forms (e.g., diastereomers, enantiomers, racemates and combinations thereof). The invention extends to these stereoisomeric forms and mixtures thereof, with respect to all compounds described by formula (I). To the extent the prior art teaches the synthesis or isolation of a particular stereoisomer, the different stereoisomeric forms of the invention may be separated from each other by methods known in the art, or a given isomer may be obtained by stereospecific or asymmetric synthesis. Tautomeric forms and mixtures of the compounds described herein are also contemplated.

Pharmaceutical composition

The pharmaceutical compositions provided herein comprise at least one compound described herein and at least one pharmaceutically acceptable excipient (e.g., a pharmaceutically acceptable carrier or diluent). In particular, contemplated pharmaceutical compositions comprise a compound described herein in an amount sufficient to treat a viral infection in a subject.

Contemplated subjects include, for example, living cells and mammals, including humans. The compounds of the present invention may be associated with or diluted by a pharmaceutically acceptable excipient (e.g., carrier or diluent) or enclosed within a carrier, which may be in the form of a capsule, sachet or other container.

Examples of suitable carriers include, but are not limited to, water, salt solutions, alcohols, polyethylene glycols, peanut oil, olive oil, gelatin, lactose, terra alba, sucrose, dextrin, magnesium carbonate, sugar, amylose, magnesium stearate, talc, gelatin, agar, pectin, gum arabic, stearic acid, lower alkyl ethers of cellulose, silicic acid, fatty acids, fatty acid amines, fatty acid mono-and diglycerides, fatty acid esters, and polyethylene oxide.

The carrier or diluent may comprise a sustained release material, such as glyceryl monostearate or glyceryl distearate, alone or with a wax.

The pharmaceutical composition may further comprise one or more pharmaceutically acceptable adjuvants, wetting agents, emulsifying agents, suspending agents, preservatives, salts for influencing osmotic pressure, buffers, sweetening agents, flavoring agents, coloring agents or any combination of the foregoing. The pharmaceutical compositions of the present invention may be formulated to provide rapid, sustained or delayed release of the active ingredient after administration to a subject by using methods known in the art.

The pharmaceutical compositions described herein may be, for example, asRemington：The Science and Practice of Pharmacy20 th edition, 2003(Lippincott Williams)&Wilkins). For example, the active compound may be admixed or diluted with a carrier, or enclosed within a carrier, which may be in the form of an ampoule, capsule or sachet. When the carrier serves as a diluent, it may be a solid, semi-solid, or liquid material that acts as a vehicle, excipient, or medium for the active compound.

The pharmaceutical compositions may be in conventional forms, such as capsules, tablets, solutions, suspensions, injections or products for topical application. In addition, the pharmaceutical compositions of the present invention may be formulated to provide a desired release profile.

The route of administration may be any route effective to transport the active compound to the appropriate or desired site of action. Suitable routes of administration include, but are not limited to, oral, nasal, pulmonary, buccal, subcutaneous, intradermal, transdermal, parenteral, rectal, depot (depot), subcutaneous injection, intravenous injection, intraurethral, intramuscular, intranasal, ophthalmic (e.g., with ophthalmic solutions), or topical (e.g., with topical ointments). The oral route is particularly suitable.

Solid oral formulations include, but are not limited to, tablets, capsules (soft or hard gelatin), dragees (containing the active ingredient in powder or pellet form), troches (troche), and lozenges (lozenes). Tablets, dragees or capsules with talc and/or carbohydrate carriers or binders and the like are particularly suitable for oral use. Exemplary carriers for tablets, dragees or capsules include lactose, corn starch and/or potato starch. Syrups or elixirs may be employed where a sweetening vehicle may be employed.

Typical tablets may be prepared by conventional tabletting techniques.

Liquid formulations include, but are not limited to, syrups, emulsions, soft gelatin and sterile injectable liquids, such as aqueous or non-aqueous liquid suspensions or solutions.

For parenteral use, particularly suitable are injectable solutions or suspensions, in particular aqueous solutions with the active compound dissolved in polyhydroxylated castor oil.

Screening method

The antiviral HIV activity and cytotoxicity of the compounds of the invention can be measured in parallel by methods disclosed in the following documents.

The cytotoxic effect of the compounds can be analyzed by measuring the proliferation of cells using 3- (4, 5-dimethylthiazol-2-yl) -2, 5-diphenyltetrazolium bromide (MTT) staining. Cells (5X 10)³Individual cells/well) will be incubated in 96-well plates with or without compound. At the end of the treatment, 20. mu.l of MTT (5mg/ml in PBS) will be added per well and incubated for another 4 hours at 37 ℃. Purple blue MTT formazan precipitate will be dissolved in a triple reagent containing 10% SDS, 5% isobutanol and 10mmol/L HCl. By measuring microThe optical density at 570nm on the titration plate was used to assess mitochondrial activity (reflecting cell growth and survival).

The effect of a compound on HIV replication in Sup-T1 cells can be determined by the method disclosed by Roda Rani et al, 2006(Archives of Biochemistry and Biophysics, Vol.456, No. 1, 2006, 12/1/2006, pp.79 to 92).

Briefly, 1 × 10 cells with 100% cell viability were assayed⁶One Sup-T1 cell was seeded in four 12-well plates of RPMI1640, 0.1% FBS. Increasing concentrations of Epap-1 peptide were added to the cells and would be infected with HIV1 at final concentrations of virus equal to 2ng of p24 per ml each_93IN101. Infected cells will be at 37 ℃ and 5% CO₂Incubate in incubator for 2 hours. After 2 hours, cells will be pelleted at 350g for 10 minutes, supernatant will be discarded, and cells will be maintained with RPMI1640 containing 10% FBS. Cells were resuspended in the same medium with increasing concentration of Epap-1 peptide and would be incubated for 96 hours. Cells will be supplemented with peptide every 24 hours. The supernatant will be collected after 96 hours and analyzed using the P24 antigen capture assay kit (SAIC Fredrick). Infection in the absence of Epap-1 would be considered 0% inhibition. Azidothymidine (AZT) will serve as a positive control.

The effect of compounds on viral entry and quantification of the virus entered can be performed on GFP expression by the following published methods: J.Virol.72, 6988(1998) by Cecilia et al, and Analytical Biochemistry, Vol.360, No. 2, 1/15 of 2007, pp.315 to 317 (Dyavar S.Ravi and Debashis Mitra).

Briefly, cells were seeded into wells of 24-well plates 1 day before the experiment. Cells will be transfected with Tat-reporter. The virus inoculum will be adjusted to 1,000-4,000TCID 50/ml in assay medium (DMEM, 10% FCS, glutamine and antibiotics) and 50. mu.l aliquots will be incubated with serial dilutions of the compound (50. mu.l) for 1 hour at 37 ℃. Reporter expression will be quantified at the appropriate time and the calculated inhibitory dose refers to the concentration of these drugs in this pre-incubation mixture.

Other relevant references for screening for antiviral HIV activity are: averett, D.R.1989.anti-HIV compound assessment by two novel high capacity assays.J.Virol. Methods 23: 263-276; schwartz, O. et al 1998; a rapid and simple colorimetric test for the study of anti HIV agents AIDS Res. and Human Retroviruses,4 (6): 441- > 447; daluge, S.M., et al 1994.5-Chloro-2',3' -deoxy-3' fluorouridine (935U83), a selective anti-human immunological virus agent with an improved metabolism and a toxicologic profile; agents and chemitherapy, 38 (7): 1590-; mitsuya and S.Border, Inhibition of the in vitro activity and cytopathic effect of human T-lymphopic virus type lymphobranched-associated virus (HLTV-111/LAV) by 2,3' -dideoxynucleosides, Proc. Natl.Acad.Sci.USA,83,1911-15 (1986); pennington et al, Peptides 1990; meek T.D et al, Inhibition of HIV-1protease in infected T-lipids by synthetic peptide analogues, Nature,343, p90 (1990); weislow et al, J.Natl.cancer Inst.81,577-586,1989; T.Mimoto et al, J.Med.Chern, 42,1789-1802, 1999; uckun et al 1998, analytical Agents and Chemotherapy 42: 383 (b); for P24 anti assay Erice et al, 1993, Antericrob. Ag. chemotherapeutics 37: 385-383; koyanagi et al, int.J. cancer,36,445-451, 1985; balzarini et al AIDS (1991),5, 21-28; connor et al, Journal of virology,1996,70, 5306-; popik et al, Journal of virology,2002,76, 4709-; harrigton et al, Journal of Virology Methods,2000,88, 111-; roos et al, Virology 2000,273, 307-; fedyuk n.v.et al; problems of Virology 1992, (3) P135; mosmann T, Decumber 1983, Journal of immunological methods,65(1-2), 55-63; SPC Cole, cancer chemistry and Pharmacology,1986,17, 259-Type 263, antibody methods and protocols (Eds: D Kinchington and R.F.Schinazi) Humana Press Inc.,2000, HIV protocols (Eds: N.L.Michael and J.H.Kim) Humana Press Inc,1999, DAIDS visual management from HIV laboratories, Publication NIH-97-3838,1997,4.HIV-1p24 antigen capture class, enzyme analysis for detection of Human immunological reagents Type 1(HIV-1) p24 in tissue culture medium-attached biological kits, Inc.

Methods of treatment

The present invention provides compounds and pharmaceutical formulations thereof for the treatment of diseases, conditions and/or disorders mediated by viral infections. The relationship between the therapeutic effect and the antiviral effect is illustrated. For example, PCT publication Nos. WO 01//07646, WO 01/65957, or WO 03/037908; US publication nos. US 4,598,095 or US 2002/0068757; EP publication nos. EP0989862 or EP 0724650; bioorganic & Medicinal Chemistry Letters,16, (6), 1712-; as well as references cited therein, all of which are incorporated herein by reference in their entirety for all purposes.

The invention further provides methods of treating a disease, condition, and/or disorder mediated by a viral infection in a subject in need thereof by administering to the subject a therapeutically effective amount of a compound or pharmaceutical composition of the invention.

Diseases, conditions and/or disorders mediated by viral infection are believed to include, but are not limited to, HIV infection, HBV infection, HCV infection, retroviral infections genetically related to HIV, AIDS, inflammatory diseases, respiratory disorders (including Adult Respiratory Distress Syndrome (ARDS), bronchitis, chronic obstructive pulmonary disease, cystic fibrosis, asthma, emphysema, rhinitis and chronic sinusitis), inflammatory bowel diseases (including crohn's disease and ulcerative colitis), multiple sclerosis, rheumatoid arthritis, transplant rejection (particularly but not limited to kidney and lung allografts), endometriosis, type I diabetes, kidney disease, chronic pancreatitis, inflammatory lung disease, chronic heart failure and bacterial infection (particularly but not limited to tuberculosis).

The compounds of the present invention, when used in appropriate combination with available drugs, can achieve a more advantageous effect than the additive effect of preventing or treating the above-mentioned diseases. In addition, the dosage administered may be reduced as compared to either drug administered alone, or the adverse effects of co-administration of drugs other than antiviral drugs may be avoided or reduced.

Method for preparing

The compounds described herein can be prepared by techniques known in the art. In addition, the compounds described herein can be prepared according to the reaction sequence described in the following schemes. Further, in the following schemes, when reference is made to a particular base, acid, reagent, solvent, coupling agent, etc., it is understood that other bases, acids, reagents, solvents, coupling agents, etc., known in the art may also be used and are therefore included in the present invention. Variations in reaction conditions, such as the temperature and/or duration of the reaction, known in the art, may be used are also within the scope of the present invention. Unless otherwise indicated, all stereoisomers of the compounds in these schemes are also included within the scope of the invention.

The compounds of the present invention may be synthesized from naturally occurring betulinic acid. Key intermediates required for the synthesis of analogs are commercially available or can be prepared by methods disclosed in the literature. For example, key intermediates in the present invention are prepared by modifying Journal of organic chemistry 2010,75, 1285-1288; journal of organic chemistry 2000,65, 3934-; tetrahedron: asymmetry 2008,19, 302-308; or Tetrahedron: prepared by the procedure disclosed in asymmetry 2003,14, 217-.

Another embodiment of the present invention provides a process for preparing a compound of formula (I) as set forth in the general scheme below. The skilled person will appreciate that the following general schemes may be employed to prepare the compounds of general formula (I) and pharmaceutically acceptable salts according to the present invention. Wherein all symbols/variables are as defined above, unless otherwise indicated.

The general synthetic process is as follows:

scheme-1:

a compound of formula (I) (wherein, R₁、R₃And R₄The same as defined above; p₁And P₂Is a protecting group, e.g., acetyl, benzyl, etc.) can be prepared as described in scheme 1. Of formula (i)The C-3 hydroxy compound can be protected under reflux conditions with a reagent such as acetic anhydride and acetic acid (or) under basic conditions with acetic anhydride with a reagent such as triethylamine or N, N-diisopropylethylamine and a catalyst such as 4-dimethylaminopyridine in a solvent such as THF and the like under reflux conditions. The C-28 acid compound of formula (ii) can be converted to the ester compound of formula (iii) by using a reagent such as benzyl bromide and a base such as potassium carbonate or sodium carbonate in a solvent such as acetonitrile or THF under reflux conditions. The C-3 acetyl and C-28 ester compounds of formula (iii) may be converted to C-20 cyclopropyl compounds of formula (iv) in the presence of a cyclopropanating agent such as diethyl zinc or dimethyl zinc with diiodomethane in a solvent such as dichloromethane or toluene and the like. The C-28 ester compound of formula (iv) can be deprotected to a C-28 acid compound of formula (v) by using (i) a catalyst such as Pd/C in a solvent such as ethyl acetate, methanol and the like under a hydrogen pressure (or) (ii) a catalyst such as Pd/C and a reagent such as ammonium formate in a solvent such as ethanol and THF. The C-28 acid compound of formula (v) may be converted to the C-17 isocyanato compound of formula (vi) in the presence of a reagent such as diphenylphosphoryl azide (DPPA) and the like in the presence of a base such as Triethylamine (TEA) or N, N-diisopropylethylamine and the like in a solvent such as toluene or 1, 4-dioxane and the like. The C-17 isocyanato compound of formula (vi) can be converted to the C-17 amine hydrochloride compound of formula (vii) in the presence of a reagent such as concentrated HCl and the like in a solvent such as 1, 4-dioxane and the like. The C-17 amine hydrochloride compound of formula (vii) may be used, for example, in the reaction of di-tert-butyl dicarbonate [ (BETA omicron)₂O]In the presence of an equivalent agent, in the presence of a base such as sodium bicarbonate (NaHCO)₃) Or sodium carbonate, etc., in the presence of a solvent such as 1, 4-dioxane: h₂O, etc. to a C-17 amine protected compound of formula (viii). The C-3 hydroxy compound of formula (viii) can be coupled with a compound of formula (ix) in a solvent such as Dichloromethane (DCM) or THF and the like using a reagent such as EDC. HCl and the like together with a catalyst such as 4-Dimethylaminopyridine (DMAP) and the like to give a C-3 ester compound of formula (x). The C-17 amine protecting compound of formula (x) may be converted to a C-17 amine compound of formula (xi) in a solvent such as 1, 4-dioxane and the like in the presence of a reagent such as 4N HCl in 1, 4-dioxane and the like. The C-17 amine compound of formula (xi) may be prepared in the presence of a reagent such as trifluoromethanesulfonic anhydride, 2, 6-lutidine and a base such as triethylamine or N, N-diisopropylethylamine in a solvent such as dichloroethylamineMethane, etc., under in situ conditions to the hydroxy compound of formula (xiii) to give the corresponding C-17 substituted amine compound of formula (xiii). The C-3 protected compound of formula (xiii) can be prepared in the presence of a reagent such as KOH or NaOH in a solvent such as methanol, THF, H₂O, etc. to give the corresponding acid compounds of the invention of formula (I).

Scheme-2:

a compound of formula (I) (wherein, R₁、R₃And R₄The same as defined above; p₁And P₂Is a protecting group, e.g., acetyl, benzyl, etc.) can be prepared as described in scheme 2. The C-3 hydroxy compound of formula (i) may be protected under reflux conditions with reagents such as acetic anhydride and acetic acid (or) under basic conditions with acetic anhydride with reagents such as triethylamine or N, N-diisopropylethylamine and a catalyst such as 4-dimethylaminopyridine under reflux conditions in a solvent such as THF and the like. The C-28 acid compound of formula (ii) may be converted to the C-17 isocyanato compound of formula (iii) in the presence of a reagent such as diphenylphosphoryl azide (DPPA) or the like in the presence of a base such as Triethylamine (TEA) or N, N-diisopropylethylamine or the like in a solvent such as toluene or 1, 4-dioxane or the like. The C-17 isocyanato compound of formula (iii) can be converted to the C-17 amine hydrochloride compound of formula (iv) in the presence of a reagent such as concentrated HCl and the like in a solvent such as 1, 4-dioxane and the like. The C-17 amine hydrochloride compound of formula (iv) may be used, for example, in the reaction of di-tert-butyl dicarbonate [ (BETA omicron)₂O]In the presence of an equivalent agent, in the presence of a base such as sodium bicarbonate (NaHCO)₃) Or sodium carbonate, etc., in the presence of a solvent such as 1, 4-dioxane: h₂O, etc. to a C-17 amine protected compound of formula (v). The C-3 hydroxy compound of formula (v) can be coupled with a compound of formula (vi) using a reagent such as EDC. HCl, etc., and a catalyst such as 4-Dimethylaminopyridine (DMAP), etc., in a solvent such as Dichloromethane (DCM) or THF, etc., to give a C-3 ester compound of formula (vii). The C-17 amine protected compound of formula (vii) may be in the presence of a reagent such as 4N HCl in 1, 4-dioxane, or the like, in a solventE.g., 1, 4-dioxane, etc., to a C-17 amine compound of formula (viii). The C-17 amine compound of formula (xiii) can be coupled with the hydroxy compound of formula (ix) in the presence of a reagent such as trifluoromethanesulfonic anhydride, 2, 6-lutidine and a base such as triethylamine or N, N-diisopropylethylamine and the like in a solvent such as dichloromethane and the like under in situ conditions to give the corresponding C-17 substituted amine compound of formula (x). The C-3 protected compound of formula (x) may be in the presence of a reagent such as KOH or NaOH in a solvent such as methanol, THF, H₂O, etc. to give the corresponding acid compounds of the invention of formula (I).

Scheme-3:

the C-17 amine compound of formula (xiv) can be coupled with the hydroxy compound of formula (xv) in the presence of a reagent such as trifluoromethanesulfonic anhydride, 2, 6-lutidine, and a base such as triethylamine or N, N-diisopropylethylamine, etc., in a solvent such as dichloromethane, etc., under in situ conditions to give the corresponding C-17 substituted amine compound of formula (xvi). The C-3 protected compound of formula (xvi) can be prepared in the presence of a reagent such as KOH or NaOH in a solvent such as methanol, THF, H₂O, etc. to yield the corresponding acid compound of formula (xvii). The silyl ether group in the compound of formula (xvii) can be deprotected with a reagent such as TBAF in THF or the like and in a solvent such as THF or the like to give the corresponding acid compound of formula (I) of the present invention.

In a further aspect, the compounds of the present invention may also contain unnatural proportions of atomic isotopes at one or more of the atoms that constitute such compounds. For example, the invention also includes isotopically-labeled variants of the invention, which are identical to those recited herein, except that one or more atoms of the compound are replaced by an atom having an atomic mass or mass number different from the atomic mass or mass number usually found in nature. All isotopes of any particular atom or element specified are contemplated as being within the scope of the compounds of the present invention and their uses. Examples of compounds that can be incorporated into the present inventionSex isotopes include isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorus, sulfur, fluorine, chlorine and iodine, e.g.²H("D")、³H、ⁿC、¹³C、¹⁴C、¹³N、¹⁵N、¹⁵O、¹⁷O、¹⁸O、³²P、³³P、³⁵S、¹⁸F、³⁶C1、¹²³I and¹²⁵I. a specific isotope is-CD₃or-C (D)₂) -. Isotopically labeled compounds of the present invention can generally be prepared by following procedures analogous to those disclosed in the schemes and/or in the examples below, by substituting an isotopically labeled reagent for a non-isotopically labeled reagent.

Abbreviations: abbreviations used throughout the specification may be summarized below in their specific meaning as follows: 1H NMR (proton nuclear magnetic resonance); hz (hertz); MHz (megahertz); DMSO-d6 (deuterated dimethyl sulfoxide); δ (delta); ppm (parts per million); s (singlet); d (bimodal); dd (double doublet); t (triplet); m (multiplet); j (coupling constant); JAB (coupling constant); ABq (AB quartet); brs (broad singlet); ml (milliliters); deg.C (degrees Celsius); mol (mole); mmol (millimole); n (equivalent weight solution); g (grams); pH (hydrogen ion activity); Pd/C (activated carbon-supported palladium); eq (equivalent); psi (pounds per square inch); ESI-MS (electrospray ionization mass spectrometry); ES-MS (electrospray mass spectrometry); m/z (mass to charge ratio); M-H- (mother mass spectrum peak minus hydrogen-); m + Na + (peak of the mother mass plus sodium +); m + H + (parent mass spectrum peak plus hydrogen +); DCM (dichloromethane); DMF (N, N-dimethylformamide); THF (tetrahydrofuran); na (Na)₂SO₄(sodium sulfate); HCl (hydrochloric acid); TLC (thin layer chromatography); percent (percent); TEMPO (2,2,6, 6-tetramethylpiperidin-1-yl) oxy); KBr (potassium bromide); DIBAL-H (diisobutylaluminum hydride); DAST (diethylaminosulfur trifluoride); TBAF (tetra-n-butylammonium fluoride); NaOH (sodium hydroxide); KOH (potassium hydroxide); edc.hcl (1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride); MTBE (methyl tert-butyl ether); HBTU (N, N' -tetramethyl-O- (1H-benzotriazol-1-yl) uronium hexafluorophosphate); h (or) hrs (hours); h (hydrogen); APCI-MS (atmospheric pressure chemical ionization mass spectrometry); DMAP (4-dimethylaminopyridine); TBDMSCl (tert-butyl dimethyl silicon chloride)An alkane).

Experiment of

The invention is further illustrated by the following examples, which are not to be construed in any way as imposing limitations upon the scope of the disclosure, but are merely illustrative. On the contrary, it is to be clearly understood that resort may be had to various other embodiments, modifications, and equivalents thereof which, after reading the description herein, may suggest themselves to those of ordinary skill in the art without departing from the spirit of the present invention. Thus, the skilled artisan will appreciate how to further carry out the disclosed experiments and examples by diversely varying the following examples, substituents, reagents or conditions.

Intermediates

Intermediate 1: preparation of (1S,3R) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1- ((1R,3aS,5aR,5bR, 7aR,9S,11aR,11bR,13aR,13bR) -3 a-amino-5 a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosaenoic acid hydro-1H-cyclopenta [ a] -9-yl) -3-benzyl ester:

step 1: synthesis of (1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -9-acetoxy-5 a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-hydro-3 aH-cyclopenta [ a]-3 a-formic acid:

to betulinic acid (200g, 0.438mol, 1.0eq) in an RB flask was added acetic acid (1600ml) and acetic anhydride (173g, 1.696mol, 3.87 eq). The reaction mixture was stirred and heated to 130 ℃ and 140 ℃ for about 4 hours. The progress of the reaction was monitored by TLC, indicating that the starting material was complete. About 1100ml of acetic acid was distilled off under vacuum. The flask was cooled to room temperature, methanol (1400ml) was slowly added and stirred at room temperature for 12-16 hours. The solid was filtered, washed with methanol (200ml) and dried in vacuo (50-55 ℃ C.) to give the title compound as a white solid (182g, 83.32% yield).

Step 2: synthesis of (1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -9-acetoxy-5 a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-hydro-3 aH-cyclopenta [ a]-3 a-carboxylic acid benzyl ester:

to (1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -9-acetoxy-5 a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-hydro-3 aH-cyclopenta [ a ] a]To a stirred solution of (E) -3 a-carboxylic acid (step 1, 180g, 0.361mol, 1.0eq) in acetonitrile (1800ml) was added potassium carbonate (99.75g, 0.721mol, 2.0 eq). The reaction mixture was stirred at room temperature and benzyl bromide (42.5ml, 0.361mol, 1.0eq) was added dropwise over 30 minutes. The reaction mixture was stirred and heated to reflux for about 6 hours. The progress of the reaction was monitored by TLC, indicating that the starting material was complete. The reaction mixture was cooled to room temperature, water (2700ml) was added and stirred for 2 hours. Ethyl acetate (2700ml) was added and stirred for 30 minutes. The organic layer was separated and the aqueous layer was extracted twice more with ethyl acetate (2x900 ml). The combined organic layers were washed with water (1800ml) and brine solution (1800 ml). The organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude compound was purified by treatment with methanol (720ml), stirred and heated to 60-65 ℃ for 30 minutes. The reaction mixture was cooled to room temperature and stirred for 2 hours. Filtering the mixtureWashed with methanol (180ml) and dried in vacuo (55-60 ℃) to give the title compound as a solid (180g, 84.7% yield).

And step 3: synthesis of (1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -9-acetoxy-5 a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-3 aH-cyclopenta [ a ] a]-3 a-Carboxylic acid benzyl ester

To (1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -9-acetoxy-5 a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-hydro-3 aH-cyclopenta [ a ] a]To a stirred solution of benzyl (3 a) -carboxylate (step 2, 120g, 0.204mol, 1.0eq) in DCM (1200ml) was added diethylzinc (75.61g, 408ml, 0.612mol, 3.0eq, 1.5M in toluene). The reaction mixture was stirred at-25 to-35 ℃ for 1 hour. Diiodomethane (81.5ml, 1.02mol, 5.0eq) was slowly added dropwise over about 45 minutes at-25 to-35 ℃. The reaction mixture was stirred at-25 to-35 ℃ for about 3 hours. The reaction mixture was slowly warmed to room temperature and stirred for about 14 hours. TLC indicated that the starting material was complete and the desired product was observed. The reaction mixture was cooled to 0-5 ℃, quenched with saturated ammonium chloride solution (600ml), and 1N HCl (240ml) was added slowly to adjust the pH (3 to 4) and stirred for 30 minutes. The organic layer was separated and the aqueous layer was extracted with DCM (600 ml). The combined organic layers were washed with 10% sodium bicarbonate solution, water (1800ml) and brine solution (1800 ml). The organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure. To the resulting solid was added diisopropyl ether (360ml), and the mixture was stirred at room temperature for 3 hours. The solid was filtered, washed with diisopropyl ether (120ml) and dried in vacuo (55-60 ℃) to give the title compound as a white solid (100g, 81.4% yield).

And 4, step 4: synthesis of (1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -9-acetoxy-5 a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-3 aH-cyclopenta [ a]3 a-formic acid:

(1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -9-acetoxy-5 a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-3 aH-cyclopenta [ a ] in methanol (200ml)]Benzyl-3 a-carboxylate (step 3, 35g, 58.13mmol, 1.0eq) was added to a suspension of 10% Pd/C (15g, 50% wet) in ethyl acetate (350 ml). The reaction mixture was hydrogenated in a parr shaker unit (parr shaker appatus) at 50psi pressure for 3 hours. TLC indicated the consumption of starting material. The reaction mixture was filtered through a celite pad, and the filtrate was evaporated under reduced pressure to give the title compound (30g) as a solid.¹H NMR(300MHz,CDCl₃)：δppm 4.51-4.46(m,1H),2.25-2.21(m,1H),2.13-0.78(m,24H),2.04(s,3H),0.98(s,3H),0.91(s,6H),0.86(s,3H),0.85(s,3H),0.84(s,3H),0.38-0.34(m,2H),0.27-0.24(m,2H)；ESI-MS：m/z 511.45(M-H)^-。

And 5: synthesis of (1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3 a-isocyanato-5 a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-hydro-1H-cyclopenta [ a ] a]-9-yl acetate:

to (1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -9-acetoxy-5 a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-3 aH-cyclopenta [ a ]]To a stirred solution of (E) -3 a-carboxylic acid (step 4, 20g, 39.00mmol, 1.0eq) in toluene (200ml) were added diphenylphosphorylazide (22.36ml, 97.50mmol, 2.5eq) and triethylamine (13.70ml, 97.50mmol, 2.5 eq). The reaction mixture was refluxed for 1 hour. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was evaporated under reduced pressure, diluted with water (250ml) and extracted with DCM (2 × 200 ml). The combined organic layers were washed with Na₂SO₄Dried, filtered and concentrated under reduced pressure. The residue was triturated with methanol (100ml), filtered and dried in vacuo to give the title compound as an off white solid (19g, 95.5% yield).¹HNMR(300MHz,CDCl₃)：δppm 4.45(m,1H),2.04(s,3H),2.03-0.78(m,25H),1.04(s,3H),0.94(s,3H),0.91(s,3H),0.88(s,3H),0.85(s,3H),0.84(s,3H),0.38-0.26(m,4H)。

Step 6: synthesis of (1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3 a-amino-5 a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-hydro-1H-cyclopenta [ a ] a]-9-alkoxide:

to (1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3 a-isocyanato-5 a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] at 0 deg.C]To a stirred solution of (e) -9-yl acetate (step 5, 19g, 37.27mmol, 1.0eq) in 1, 4-dioxane (190ml) was added concentrated HCl (57 ml). The reaction mixture was stirred at 50-60 ℃ overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was evaporated under reduced pressure and co-distilled with acetonitrile (100ml) to obtain the title compound (20g) as a solid without further distillationThe product can be used in the next step. ES-MS: m/z 442.20(M + H)⁺。

And 7: synthesis of ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -9-hydroxy-5 a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-3 aH-cyclopenta [ a ] a]-3 a-yl) carbamic acid tert-butyl ester:

to (1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3 a-amino-5 a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] at 0 deg.C]To a stirred solution of-9-alkoxide (step 6, 20g, 41.823mmol, 1.0eq) in 1, 4-dioxane (200ml) was added a saturated solution of sodium bicarbonate (100ml) and di-tert-butyl dicarbonate (14.41ml, 62.734mmol, 1.5 eq). The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was evaporated under reduced pressure, diluted with water and extracted with DCM (2 × 200 ml). The combined organic layers were washed with Na₂SO₄Dried, filtered and concentrated under reduced pressure. The residue was purified by silica gel column chromatography with 10% ethyl acetate in hexanes as eluent to give the title compound (9g, 44.57% over two steps) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 3.23-3.16(m,1H),2.54-2.51(m,1H),2.42-2.25(m,2H),2.0-1.80(m,2H),1.73-0.67(m,20H),1.42(s,9H),1.01(s,3H),0.97(s,6H),0.91(s,3H),0.85(s,3H),0.77(s,3H),0.40-0.25(m,4H)；ES-MS：m/z 564.17(M+Na)⁺。

And 8: synthesis of (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- ((tert-butoxycarbonyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-hydro-1H-cyclopenta [ a ] a]-9-yl) ester:

to ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -9-hydroxy-5 a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosanyl-3 aH-cyclopenta [ a ] at 0 deg.C]Tert-butyl (3 a-yl) carbamate (step 7,9g, 16.609mmol, 1.0eq) to a stirred solution in dichloromethane (90ml) was added (1S,3R) -3- ((benzyloxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid (prepared according to the procedure described in WO2011/007230a2, 8.71g, 33.21mmol, 2.0eq), 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride (edc.hcl) (9.55g, 49.82mmol, 3.0eq) and 4- (dimethylamino) pyridine (0.81g, 6.643mmol, 0.4 eq). The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was diluted with water (100ml) and extracted with dichloromethane (2 × 100 ml). The combined organic layers were washed with Na₂SO₄Dried, filtered and concentrated under reduced pressure. The crude compound was purified by silica gel column chromatography eluting with 10% ethyl acetate: hexane as eluent to give the title compound (11g, 84.29% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 7.34(m,5H),5.15,5.09(ABq,J_AB＝12.3Hz,2H),4.44(dd,J＝11.1,4.5Hz,1H),4.17(s,1H),2.84-2.73(m,2H),2.69-2.58(m,1H),2.57-2.51(m,1H),2.42-2.23(m,2H),2.07-1.81(m,3H),1.72-0.76(m,20H),1.42(s,9H),1.34(s,3H),1.0(s,3H),0.96(s,6H),0.91(s,3H),0.89(s,3H),0.87(s,3H),0.85(s,3H),0.40-0.25(m,4H)；ES-MS：m/z 786.47(M+H)⁺。

And step 9: synthesis of (1S,3R) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3 a-amino-5 a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-hydro-1H-cyclopenta [ a ] a]-9-yl) -3-benzyl ester:

to (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- ((tert-butoxycarbonyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) ester (step 8,11g, 13.99mmol, 1.0eq) to a stirred solution in 1, 4-dioxane (11ml) was added 4N HCl in 1, 4-dioxane (33 ml). The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was evaporated under reduced pressure and the residue was basified with saturated sodium bicarbonate solution and extracted with dichloromethane (3 × 100 ml). The combined organic layers were washed with water and Na₂SO₄Drying, filtration and evaporation under reduced pressure gave the title compound (9g, 93.84% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 7.34(m,5H),5.15,5.09(ABq,J_AB＝12.3Hz,2H),4.45(m,1H),2.81-2.73(m,2H),2.65-2.62(m,1H),2.08-1.88(m,3H),1.74-0.78(m,23H),1.34(s,3H),1.03(s,3H),0.96(s,6H),0.91(s,3H),0.87(s,3H),0.85(s,3H),0.84(s,3H),0.37-0.22(m,4H)；ES-MS：m/z 686.45(M+H)⁺。

Intermediate 2: preparation of tert-butyl (2-methyl-1-oxoprop-2-yl) carbamate:

step 1: synthesis of tert-butyl (1-hydroxy-2-methylpropan-2-yl) carbamate:

to a stirred solution of 2-amino-2-methylpropan-1-ol (10g, 112.17mmol, 1.0eq) in dichloromethane (120ml) and methanol (100ml) were added triethylamine (1.57ml, 11.217mmol, 0.1eq) and di-tert-butyl dicarbonate (30.92ml, 134.60mmol, 1.2 eq). The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was evaporated under reduced pressure, water (100ml) was added and the compound was extracted with DCM (2 × 50 ml). The organic layer was separated, dried over sodium sulfate, filtered and concentrated under reduced pressure. The residue was triturated with n-hexane, the solid filtered and dried in vacuo to give the title compound (14.0g, 66% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 4.66(s,1H),4.04(s,1H),3.58(d,J＝6.3Hz,2H),1.43(s,9H),1.25(s,6H)；ES-MS：m/z 189.80(M+H)⁺。

Step 2: synthesis of tert-butyl (2-methyl-1-oxoprop-2-yl) carbamate:

to a stirred solution of tert-butyl (1-hydroxy-2-methylpropan-2-yl) carbamate (step 1, 4g, 21.134mmol, 1.0eq) in dichloromethane (50ml) was added TEMPO (0.098g, 0.634mmol, 0.03eq), KBr (0.251g, 2.1134mmol, 0.1eq), sodium bicarbonate (10.82g, 128.91mmol, 6.1eq), 10% NaOCl (26ml), followed by water (1.05 ml). The reaction mixture was stirred at room temperature for about 30 minutes. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was diluted with dichloromethane (2 × 50ml) and washed with water (50 ml). The organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure to give the title compound (7.0g) as a colorless liquid. The obtained compound can be directly used in the next step without further purification;¹H NMR(300MHz,CDCl₃)：δppm 9.43(s,1H),4.98(s,1H),1.44(s,9H),1.33(s,6H)；ES-MS：m/z 187.78(M+H)⁺。

intermediate 3: preparation of (S) -4- (1-chloropropan-2-yl)Thiomorpholine 1, 1-dioxide:

step 1: synthesis of (S) -4- (1-hydroxypropan-2-yl) thiomorpholine 1, 1-dioxide:

to a stirred solution of (S) -2-aminopropan-1-ol (1.0g, 13.31mmol, 1.0eq) in 1, 4-dioxane (5ml) and ethanol (5ml) were added divinylsulfone (2.67ml, 26.62mmol, 2.0eq) and triethylamine (5.61ml, 39.93mmol, 3.0 eq). The reaction mixture was heated to 85 ℃ for about 3 hours. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was evaporated under reduced pressure and the crude compound was purified by silica gel column chromatography with 5% methanol in DCM as eluent to give the title compound (2.0g, 80% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 3.51-3.34(m,2H),3.22-3.16(m,2H),3.13-3.06(m,4H),3.03-2.90(m,3H),2.60(s,1H),0.97(d,J＝6.9Hz,3H)；ES-MS：m/z 193.97(M+H)⁺。

Step 2: synthesis of (S) -4- (1-chloropropan-2-yl) thiomorpholine 1, 1-dioxide:

to a stirred solution of (S) -4- (1-hydroxypropan-2-yl) thiomorpholine 1, 1-dioxide (step 1, 1.3g, 6.72mmol, 1.0eq) in 1, 2-dichloroethane (13ml) was added thionyl chloride (1.47ml, 20.18mmol, 3.0 eq). The reaction mixture was heated to reflux for about 3 hours. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was evaporated under reduced pressure, diluted with dichloromethane (20ml), washed with saturated sodium bicarbonate solution and water. Na for organic layer₂SO₄Dried, filtered and concentrated under reduced pressure. Crude compound is passed through silica gel columnPurification by chromatography eluting with 3% methanol in dichloromethane afforded the title compound (0.925g, 64.91% yield) as a colourless liquid.¹H NMR(300MHz,CDCl₃)：δppm 4.06-3.99(m,1H),3.12-3.05(m,8H),2.84-2.77(m,1H),2.72-2.66(m,1H),1.51(d,J＝6.6Hz,3H)；ES-MS：m/z 212.34(M+H)⁺。

Intermediate 4: preparation of (R) -4- (1-hydroxy-3-methylbut-2-yl) thiomorpholine 1, 1-dioxide:

to a stirred solution of (R) -2-amino-3-methylbutan-1-ol (3g, 29.069mmol, 1.0eq) in ethanol (10ml) and 1, 4-dioxane (10ml) was added triethylamine (12.25ml, 87.207mmol, 3.0eq) followed by divinyl sulfone (6.869g, 58.139mmol, 2.0 eq). The reaction mixture was refluxed for about 3 hours. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was evaporated under reduced pressure. The crude compound was purified by silica gel column chromatography with 2% methanol in dichloromethane as eluent to give the title compound (3.6g, 55.9% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 3.69(m,1H),3.46-3.39(m,1H),3.32-3.14(m,4H),3.05-3.03(m,4H),2.45-2.38(m,1H),1.85-1.73(m,1H),1.02(d,J＝6.6Hz,3H),0.89(d,J＝6.6Hz,3H)。

Intermediate 5: preparation of methyl (R) -2- (1, 1-thiomorpholino) -3-hydroxypropionate:

to a stirred solution of D-serine methyl ester hydrochloride (4.0g, 25.70mmol, 1.0eq) in 1, 4-dioxane (20ml) and ethanol (20ml) was added triethylamine (14.34ml, 102.8mmol, 4.0eq) followed by divinyl sulfone (5.15ml, 51.4mmol, 2.0 eq). The reaction mixture was heated to 85 ℃ for 3 hours. TLC indicated that the starting material was complete and the desired product was observed. Will reactThe mixture was cooled to room temperature and concentrated under reduced pressure. The crude compound was purified by silica gel column chromatography using a 5-10% methanol/dichloromethane gradient to give the title compound (5.8g, 95% yield) as a white solid.¹H NMR(300MHz,CDCl₃)：δppm 3.90-3.84(m,1H),3.81-3.73(m,1H),3.76(s,3H),3.54(dd,J＝8.1,6.0Hz,1H),3.40-3.30(m,2H),3.17-3.13(m,1H),3.09-3.06(m,5H),2.48(s,1H)。

Intermediate 6: preparation of (R) -4- (1- ((tert-butyldimethylsilyl) oxy) -3-hydroxypropan-2-yl) thio Morpholine 1, 1-dioxide:

step 1: synthesizing (S) -2- (1, 1-thiomorpholino) -3-hydroxypropionic acid methyl ester:

to a stirred solution of L-serine methyl ester hydrochloride (4g, 25.707mmol, 1.0eq) in 1, 4-dioxane (40ml) and ethanol (40ml) was added triethylamine (14.3ml, 102.828mmol, 4.0eq) and divinyl sulfone (6g, 51.414mmol, 2.0 eq). The reaction mixture was stirred at 85 ℃ for about 3 hours. TLC indicated that the starting material was complete and the desired product was observed. The reaction mixture was concentrated under reduced pressure. The crude compound was purified by silica gel column chromatography using a 40-80% ethyl acetate/hexane gradient to give the title compound (5g, 81.96% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 3.91-3.83(m,1H),3.81-3.73(m,1H),3.76(s,3H),3.54(dd,J＝8.1,6.0Hz,1H),3.40-3.31(m,2H),3.19-3.02(m,6H),2.42(s,1H)；ES-MS：m/z 238.04(M+H)⁺。

Step 2: synthesis of methyl (S) -3- ((tert-butyldimethylsilyl) oxy) -2- (1, 1-thiomorpholino) propionate:

to a stirred solution of (S) -methyl 2- (1, 1-thiomorpholino) -3-hydroxypropionate (step 1, 2.5g, 10.536mmol, 1.0eq) in DCM (37.5ml) was added triethylamine (4.4ml, 31.609mmol, 3.0eq), 4- (dimethylamino) pyridine (0.064g, 0.525mmol, 0.05eq) at 0 ℃, followed by tert-butyldimethylchlorosilane (2.5g, 16.858mmol, 1.6 eq). The reaction mixture was stirred at room temperature overnight. The reaction mixture was diluted with water and the aqueous layer was separated. The organic layer was washed with saturated ammonium chloride solution, 5% sodium bicarbonate solution, water and brine solution. The organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified by silica gel column chromatography using a 0-40% ethyl acetate/hexanes gradient to give the title compound (3g, 81% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 3.93-3.90(m,2H),3.72(s,3H),3.53-3.49(m,1H),3.29-3.24(m,4H),3.08-3.02(m,4H),0.87(s,9H),0.05(s,6H)。

And step 3: synthesis of (R) -4- (1- ((tert-butyldimethylsilyl) oxy) -3-hydroxypropan-2-yl) thiomorpholine 1, 1-dioxide:

to a stirred solution of methyl (S) -3- ((tert-butyldimethylsilyl) oxy) -2- (1, 1-thiomorpholino) propanoate (step 2, 3.5g, 9.956mmol, 1.0eq) in methanol (70ml) was added sodium borohydride (7.53g, 199.129mmol, 20eq) portionwise at 0 ℃. The reaction mixture was stirred at room temperature for about 2 hours. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was neutralized with 1N HCl, the organic phase was evaporated under reduced pressure and the aqueous layer was extracted with ethyl acetate (2 × 100 ml). The combined organic layers were washed with water (100ml), brine solution (100ml), dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude compound was purified by column chromatography on silica gel using a gradient of 0-10% methanol in dichloromethane to give the title compound (3g, 93.7% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 3.73-3.68(m,1H),3.67-3.57(m,1H),3.56-3.47(m,1H),3.46-3.33(m,2H),3.18-3.01(m,7H),2.99-2.89(m,1H),0.89(s,9H),0.06(s,6H)；ES-MS：m/z 323.64(M+H)⁺。

Intermediate 7: preparation of (S) -4- (1-hydroxy-3-methylbut-2-yl) thiomorpholine 1, 1-dioxide:

to a stirred solution of (S) -2-amino-3-methylbutan-1-ol (2.0g, 19.398mmol, 1.0eq) in ethanol (10ml) and 1, 4-dioxane (10ml) was added divinyl sulfone (3.89ml, 38.797mmol, 2.0eq) and triethylamine (8.178ml, 58.19mmol, 3.0 eq). The reaction mixture was heated to reflux for about 5 hours. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was evaporated under reduced pressure. The residue was purified by silica gel column chromatography with 2% methanol in DCM as eluent to give the title compound (4.0g, 95.2% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm3.74-3.68(m,1H),3.45-3.38(m,1H),3.32-3.14(m,4H),3.05-3.02(m,4H),2.47-2.36(m,2H),1.83-1.75(m,1H),1.02(d,J＝6.6Hz,3H),0.89(d,J＝6.6Hz,3H)；ES-MS：m/z 221.85(M+H)⁺。

Intermediate 8: preparation of 4- ((2R,3R) -3- ((tert-butyldimethylsilyl) oxy) -1-hydroxybut-2-yl) Thiomorpholine 1, 1-dioxide:

step 1: synthesis of L-threonine methyl ester:

to a stirred solution of L-threonine (10g, 83.94mmol, 1.0eq) in methanol (100ml) at 0 deg.C was added thionyl chlorideChlorine (18.38ml, 251.84mmol, 3.0 eq). The reaction mixture was heated to reflux for about 3 hours. TLC indicated that the starting material was complete and the desired product was observed. The reaction mixture was evaporated under reduced pressure, and ethyl acetate (100ml) and triethylamine (35.39ml, 251.84mmol, 3.0eq) were added to the reaction mixture to form a precipitate. The precipitate formed was collected by filtration, and the filtrate was evaporated under reduced pressure to give the title compound (8g) as an oil. The resulting compound was used in the next step without further purification.¹H NMR(300MHz,DMSO-d6)：δppm 4.62(s,1H),3.85-3.82(m,1H),3.61(s,3H),3.16(d,J＝3.9Hz,1H),1.08(d,J＝6.3Hz,3H)；ES-MS：m/z134.07(M+H)⁺。

Step 2: synthesizing (2S,3R) -2- (1, 1-thiomorpholino) -3-hydroxy methyl butyrate:

to a stirred solution of L-threonine methyl ester (step 1, 2.5g, 18.77mmol, 1.0eq) in ethanol (25ml) and 1, 4-dioxane (25ml) was added triethylamine (7.91ml, 56.31mmol, 3.0eq) and divinyl sulfone (5.61ml, 37.55mmol, 2.0 eq). The reaction mixture was heated to reflux for about 3 hours. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was evaporated under reduced pressure. The residue was purified by silica gel column chromatography eluting with 2% methanol in DCM to give the title compound (4g, 85.1% yield) as a solid.¹H NMR(300MHz,CDCl₃)：δppm 4.13-3.96(m,1H),3.76(s,3H),3.42-2.90(m,9H),1.21(d,J＝6.0Hz,3H)；ES-MS：m/z 252.26(M+H)⁺。

And step 3: synthesis of methyl (2S,3R) -3- ((tert-butyldimethylsilyl) oxy) -2- (1, 1-thiomorpholino) butyrate:

to a stirred mixture of methyl (2S,3R) -2- (1, 1-thiomorpholino) -3-hydroxybutyrate (step 2, 1.5g, 5.976mmol, 1.0eq) in DCM (15ml) and DMF (10ml) was addedImidazole (0.813g, 11.952mmol, 2.0eq) and tert-butyldimethylsilyl chloride (1.35g, 8.964mmol, 1.5eq) were added to the solution. The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was diluted with water (20ml) and extracted with DCM (2 × 30 ml). The combined organic layers were separated and washed with Na₂SO₄Dried, filtered and concentrated under reduced pressure. The crude compound was purified by column chromatography on silica gel eluting with 1% methanol in DCM to give the title compound (0.6g, 27.52% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 4.39(m,1H),3.73(s,3H),3.64-3.56(m,2H),3.30-3.24(m,3H),3.06-3.03(m,4H),1.26(d,J＝6.3Hz,3H),0.86(s,9H),0.08(s,3H),0.04(s,3H)；ES-MS：m/z 388.10(M+Na)⁺。

And 4, step 4: synthesis of 4- ((2R,3R) -3- ((tert-butyldimethylsilyl) oxy) -1-hydroxybut-2-yl) thiomorpholine 1, 1-dioxide:

DIBAL-H (6.5ml, 9.847mmol, 6.0eq, 1.5M in toluene) was added to a stirred solution of methyl (2S,3R) -3- ((tert-butyldimethylsilyl) oxy) -2- (1, 1-thiomorpholino) butyrate (step 3, 0.6g, 1.641mmol, 1.0eq) in DCM (12ml) at-78 ℃. The reaction mixture was stirred at the same temperature for about 4 hours. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was quenched with saturated ammonium chloride solution and extracted with ethyl acetate (2 × 30 ml). The organic layer was washed with water and Na₂SO₄Dried, filtered and concentrated under reduced pressure. The residue was purified by silica gel column chromatography eluting with 2% methanol in DCM to give the title compound (0.442g, 79.92% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 4.11-4.03(m,1H),3.71-3.64(m,2H),3.55-3.39(m,4H),3.19-3.08(m,4H),2.81-2.74(m,1H),2.49(s,1H),1.30(d,J＝6.0Hz,3H),1.0(s,9H),0.20(s,6H)；ESI-MS：m/z 360.05(M+Na)⁺。

Intermediate 9: preparation of (S) -4- (1- ((S)Tert-butyldimethylsilyl) oxy) -3-hydroxypropan-2-yl) thio Morpholine 1, 1-dioxide:

step 1: synthesis of methyl (R) -3- ((tert-butyldimethylsilyl) oxy) -2- (1, 1-thiomorpholino) propanoate:

to a stirred solution of (R) -methyl 2- (1, 1-thiomorpholino) -3-hydroxypropionate (intermediate 5, 4.4g, 18.54mmol, 1.0eq) in DCM (45ml) was added triethylamine (7.7ml, 55.63mmol, 3.0eq), 4- (dimethylamino) pyridine (0.113g, 0.92mmol, 0.05eq) and tert-butyldimethylchlorosilane (4.4g, 29.67mmol, 1.6eq) at 0 ℃. The reaction mixture was stirred at room temperature overnight. TLC indicated that the starting material was complete and the desired product was observed. The reaction mixture was diluted with DCM (150ml) and washed with water (100ml) and brine solution (100 ml). The organic layer was dried over sodium sulfate, filtered and evaporated under reduced pressure. The crude compound was purified by column chromatography on silica gel using a gradient of 0-40% ethyl acetate in hexanes to give the title compound (5g, 77% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 3.93-3.90(m,2H),3.72(s,3H),3.51(t,J＝5.7Hz,1H),3.26-3.24(m,4H),3.06-3.02(m,4H),0.87(s,9H),0.05(s,6H)；ES-MS：m/z 352.55(M+H)⁺。

Step 2: synthesis of (S) -4- (1- ((tert-butyldimethylsilyl) oxy) -3-hydroxypropan-2-yl) thiomorpholine 1, 1-dioxide:

to methyl (R) -3- ((tert-butyldimethylsilyl) oxy) -2- (1, 1-thiomorpholino) propionate (step 1,5g, 14.223mmol, 1.0eq) in a stirred solution of methanol (100ml) was added portionwise sodium borohydride (10.76g, 284.47mmol, 20.0 eq). The reaction mixture was stirred at room temperature for about 2 hours. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was cooled to 0 ℃, neutralized with 1N HCl and the organic phase evaporated under reduced pressure. The aqueous layer was extracted with ethyl acetate (2 × 150 ml). The combined organic layers were washed with water (150ml) and brine solution (150 ml). The organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude compound was purified by column chromatography on silica gel using a gradient of 0-10% methanol in dichloromethane to give the title compound (4g, 87% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 3.75-3.62(m,1H),3.61-3.55(m,1H),3.54-3.46(m,1H),3.42-3.33(m,2H),3.17-3.10(m,2H),3.10-3.02(m,5H),3.0-2.90(m,1H),2.50(s,1H),0.90(s,9H),0.06(s,6H)；ES-MS：m/z 324.56(M+H)⁺。

Intermediate 10: preparation of (S) -4- (1-fluoro-3-hydroxypropan-2-yl) thiomorpholine 1, 1-dioxide:

step 1: synthesis of (S) -4- (1- ((tert-butyldimethylsilyl) oxy) -3-fluoropropan-2-yl) thiomorpholine 1, 1-dioxide:

to a stirred solution of (R) -4- (1- ((tert-butyldimethylsilyl) oxy) -3-hydroxypropan-2-yl) thiomorpholine 1, 1-dioxide (intermediate 6, 7.5g, 23.291mmol, 1.0eq) in DCM (150ml) was added DAST (3ml, 23.291mmol, 1.0eq) at 0 ℃. The reaction mixture was stirred at room temperature for about 1 hour. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was quenched with saturated sodium carbonate solution and extracted with DCM. The organic layer was washed with water, dried over sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified by silica gel column chromatography using 0-A gradient of 20% ethyl acetate in hexanes gave the title compound (1.8g, 23.8% yield) as a colorless liquid.¹H NMR(300MHz,CDCl₃)：δppm 4.67-4.64(m,1H),4.51-4.49(m,1H),3.79-3.71(m,2H),3.25-3.22(m,3H),3.14-2.87(m,6H),0.90(s,9H),0.07(s,6H)；ES-MS：m/z 326.46(M+H)⁺。

Step 2: synthesis of (S) -4- (1-fluoro-3-hydroxypropan-2-yl) thiomorpholine 1, 1-dioxide:

to a stirred solution of (S) -4- (1- ((tert-butyldimethylsilyl) oxy) -3-fluoropropan-2-yl) thiomorpholine 1, 1-dioxide (step 1, 1.8g, 5.538mmol, 1.0eq) in THF (36ml) at 0 ℃ was added a solution of 1.0M TBAF in THF (6.64ml, 6.646mmol, 1.2 eq). The reaction mixture was stirred at room temperature for about 4 hours. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was diluted with DCM and washed with saturated sodium bicarbonate solution and water. The organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude compound was purified by column chromatography on silica gel using a 0-4% methanol/dichloromethane gradient to give the title compound (0.950g, 81.9% yield) as a colorless liquid.¹HNMR(300MHz,CDCl₃)：δppm 4.65-4.63(m,1H),4.49-4.47(m,1H),3.66-3.64(m,2H),3.39-3.30(m,2H),3.21-3.12(m,3H),3.11-3.05(m,4H),2.20(s,1H)；ES-MS：m/z 212.34(M+H)⁺。

Intermediate 11: preparation of (R) -4- (1-fluoro-3-hydroxypropan-2-yl) thiomorpholine 1, 1-dioxide:

step 1: synthesis of (R) -4- (1- ((tert-butyldimethylsilyl) oxy) -3-fluoropropan-2-yl) thiomorpholine 1, 1-dioxide:

to a stirred solution of (S) -4- (1- ((tert-butyldimethylsilyl) oxy) -3-hydroxypropan-2-yl) thiomorpholine 1, 1-dioxide (intermediate 9, 6g, 18.633mmol, 1.0eq) in DCM (120ml) was added DAST (1.83ml, 18.633mmol, 1.0eq) at 0 ℃. The reaction mixture was stirred at 0 ℃ for about 1 hour, brought to room temperature and stirred for about 1 hour. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was cooled to 0 ℃, quenched with saturated sodium carbonate solution and extracted with DCM (100 ml). The organic layer was washed with water, brine solution (100ml), dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude compound was purified by silica gel column chromatography using a 0-20% ethyl acetate/hexanes gradient to give the title compound (1.3g, 21.5% yield) as a colorless liquid.¹H NMR(300MHz,CDCl₃)：δppm 4.67-4.64(m,1H),4.51-4.49(m,1H),3.75(dd,J＝6.0,1.2Hz,2H),3.25-3.22(m,4H),3.04-2.90(m,5H),0.89(s,9H),0.06(s,6H)；ES-MS：m/z 326.53(M+H)⁺。

Step 2: synthesis of (R) -4- (1-fluoro-3-hydroxypropan-2-yl) thiomorpholine 1, 1-dioxide:

to a stirred solution of (R) -4- (1- ((tert-butyldimethylsilyl) oxy) -3-fluoropropan-2-yl) thiomorpholine 1, 1-dioxide (step 1, 1.3g, 4.0mmol, 1.0eq) in THF (26ml) was added TBAF (4.8ml, 4.80mmol, 1.2eq, 1.0M in THF) at 0 ℃. The reaction mixture was stirred at room temperature for about 4 hours. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was diluted with DCM (100ml) and washed with saturated sodium bicarbonate solution and water. The organic layer was separated, dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude compound was purified by column chromatography on silica gel using a 0-3% methanol/dichloromethane gradient to give the title compound (0.650g, 77% yield) as a colorless liquid.¹H NMR(300MHz,CDCl₃)：δppm 4.65-4.63(m,1H),4.49-4.47(m,1H),3.66-3.64(m,2H),3.39-3.30(m,2H),3.22-3.12(m,3H),3.11-3.03(m,4H),2.19(s,1H)；ES-MS：m/z 212.34(M+H)⁺。

Intermediate 12: preparation of (S) -2- (4- (isopropylsulfonyl) piperazin-1-yl) propan-1-ol:

step 1: synthesis of tert-butyl 4- (isopropylsulfonyl) piperazine-1-carboxylate:

a mixture of piperazine-1-carboxylic acid tert-butyl ester (5g, 26.84mmol, 1.0eq) and DCM (100ml) was cooled to-25 deg.C, triethylamine (3.25g, 32.21mmol, 1.2eq) was slowly added dropwise, followed by isopropyl sulfonyl chloride (4.4g, 30.87mmol, 1.15eq) was slowly added dropwise. The reaction mixture was slowly warmed to room temperature and stirred overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was cooled to 0 ℃, neutralized with 1N HCl solution and extracted with DCM (3 × 400 ml). The combined organic layers were washed with water (100ml), dried over sodium sulfate, filtered and concentrated under reduced pressure to give the title compound (6.9g, 87.92% yield) as a yellow solid.¹H NMR(300MHz,CDCl₃)：δppm 3.48-3.46(m,4H),3.33-3.30(m,4H),3.23-3.14(m,1H),1.46(s,9H),1.34(d,J＝6.9Hz,6H)；ES-MS：m/z 315.45(M+Na)⁺。

Step 2: synthesis of 1- (isopropylsulfonyl) piperazine:

to a stirred solution of tert-butyl 4- (isopropylsulfonyl) piperazine-1-carboxylate (step 1, 6.9g, 23.60mmol, 1.0eq) in 1, 4-dioxane (30ml) was added 4N HCl/1, 4-dioxane (30ml) at 0 ℃. The reaction mixture was stirred at room temperature for about 3 hours. TLC indicated that starting material had been consumed and the desired product was observed.The reaction mixture was evaporated under reduced pressure, cooled to 0 ℃, saturated sodium bicarbonate solution was added and extracted with DCM (3 × 400 ml). The combined organic layers were washed with water (150ml), dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude compound was purified by column chromatography on silica gel using a 0-10% methanol/dichloromethane gradient. Fractions containing the expected product were combined and concentrated under reduced pressure to give the title compound (2.8g, 62.2% yield) as a yellow semi-solid.¹H NMR(300MHz,CDCl₃)：δppm 3.35-3.32(m,4H),3.23-3.14(m,1H),2.92-2.89(m,4H),1.34(d,J＝6.9Hz,6H)；ES-MS：m/z 193.42(M+H)⁺。

And step 3: synthesis of methyl (S) -2- (4- (isopropylsulfonyl) piperazin-1-yl) propionate:

to a stirred solution of methyl (R) -2-hydroxypropionate (1.461g, 14.042mmol, 1.5eq) in DCM (40ml) was added trifluoromethanesulfonic anhydride (2.67ml, 15.915mmol, 1.7eq) at-78 ℃. The reaction mixture was stirred at-78 ℃ for about 45 minutes, at which time 2, 6-lutidine (1.85ml, 15.915mmol, 1.7eq) was added and stirred at the same temperature for about 45 minutes. To the reaction mixture was added a solution of 1- (isopropylsulfonyl) piperazine (step 2, 1.8g, 9.361mmol, 1.0eq) in DCM (10ml) at-78 ℃ followed by triethylamine (3.9ml, 28.085mmol, 3.0 eq). The reaction mixture was stirred at 0 ℃ for about 1 hour and at room temperature for about 3 hours. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was cooled to 0 ℃, quenched with saturated sodium bicarbonate solution and extracted with DCM (3 × 250 ml). The combined organic layers were washed with water (100ml), dried over sodium sulfate, filtered and concentrated under reduced pressure to give the title compound (1.8g, 69.2% yield) as a pale yellow oil.¹H NMR(300MHz,CDCl₃)：δppm 3.71(s,3H),3.43-3.31(m,5H),3.23-3.14(m,1H),2.71-2.59(m,4H),1.34(d,J＝6.9Hz,6H),1.30(d,J＝7.2Hz,3H)；ES-MS：m/z 279.40(M+H)⁺。

And 4, step 4: synthesis of (S) -2- (4- (isopropylsulfonyl) piperazin-1-yl) propan-1-ol:

to a stirred solution of methyl (S) -2- (4- (isopropylsulfonyl) piperazin-1-yl) propionate (step 3, 1.8g, 6.466mmol, 1.0eq) in methanol (100ml) was added sodium borohydride (6.11g, 161.65mmol, 25.0eq) portionwise at 0 ℃. The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was cooled to 0 ℃, the pH was adjusted to 6.0 with 1N HCl and evaporated under reduced pressure. The reaction mixture was cooled to 0 ℃, saturated sodium bicarbonate solution was added and extracted with DCM (3 × 250 ml). The combined organic layers were washed with water (100ml), dried over sodium sulfate, filtered and concentrated under reduced pressure to give the title compound (1.0g, 61.8% yield) as a liquid.¹H NMR(300MHz,CDCl₃)：δppm 3.55-3.39(m,6H),3.24-3.13(m,1H),3.01-2.90(m,1H),2.87-2.78(m,2H),2.65-2.57(m,2H),1.35(d,J＝6.6Hz,6H),0.99(d,J＝6.9Hz,3H)；ES-MS：m/z 251.58(M+H)⁺。

Intermediate 13: preparation of 4- ((2R,3S) -3-fluoro-1-hydroxybut-2-yl) thiomorpholine 1, 1-dioxide:

step 1: synthesizing (2R,3S) -2- (1, 1-thiomorpholino) -3-fluorobutyric acid methyl ester:

to a stirred solution of methyl (2S,3R) -2- (1, 1-thiomorpholino) -3-hydroxybutyrate (intermediate 8-step 2, 15g, 59.76mmol, 1.0eq) in DCM (150ml) was added DAST (8.68ml, 65.73mmol, 1.1eq) at 0 ℃. The reaction mixture was stirred at the same temperature for about 30 minutes and at room temperature for about 1 hour. TLC indicated that starting material had been consumed and the desired product was observed. Mixing the reactionThe material was cooled to 0 ℃, saturated sodium bicarbonate solution was added and extracted with DCM (2 × 200 ml). The organic layer was washed with water, dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude compound was purified by silica gel column chromatography with 1% methanol/dichloromethane as eluent to give the title compound (8g, 52.94% yield) as a liquid.¹H NMR(300MHz,CDCl₃)：δppm 5.22-5.13(m,0.5H),5.06-4.98(m,0.5H),3.77(s,3H),3.44-3.22(m,5H),3.10-2.96(m,4H),1.45(d,J＝6.3Hz,1.5H),1.37(d,J＝6.3Hz,1.5H)；ES-MS：m/z 254.46(M+H)⁺。

Step 2: synthesis of 4- ((2R,3S) -3-fluoro-1-hydroxybut-2-yl) thiomorpholine 1, 1-dioxide:

to a stirred suspension of lithium aluminium hydride (3.59g, 94.86mmol, 3.0eq) in THF (160ml) was added methyl (2R,3S) -2- (1, 1-thiomorpholino) -3-fluorobutyrate (step 1, 8g, 31.62mmol, 1.0eq) at 0 deg.C. The reaction mixture was stirred at 0 ℃ for about 1 hour, then at room temperature for about 2 hours. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was cooled to 0 ℃, quenched slowly with water (3.59ml), 10% NaOH solution (3.59ml) and then water (10.59ml) and stirred for about 2 hours. The precipitate formed was filtered and the filtrate was concentrated under reduced pressure. The residue was purified by column chromatography on silica gel using a 0-2% methanol/dichloromethane gradient to give the title compound (1g, 14.06% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 4.57-4.51(m,0.5H),4.40-4.33(m,0.5H),3.94-3.83(m,2H),3.30-3.22(m,3H),3.18-3.0(m,6H),1.17(d,J＝6.9Hz,3H)；ES-MS：m/z 226.48(M+H)⁺。

Intermediate 14: preparation of 4- ((2S,3S) -3- ((tert-butyldimethylsilyl) oxy) -1-hydroxybut-2-yl) Thiomorpholine 1, 1-dioxide:

step 1: synthesizing (2R,3S) -2- (1, 1-thiomorpholino) -3-hydroxy methyl butyrate:

to a stirred solution of D-threonine methyl ester hydrochloride (13g, 76.65mmol, 1.0eq) in 1, 4-dioxane (78ml) and ethanol (78ml) was added divinyl sulfone (19.18ml, 191.62mmol, 2.5eq) followed by triethylamine (53.4ml, 383.25mmol, 5.0 eq). The reaction mixture was heated to reflux for about 6 hours. TLC indicated that the starting material was complete and the desired product was observed. The reaction mixture was evaporated under reduced pressure. The crude compound was purified by column chromatography on silica gel using a 0-6% methanol/dichloromethane gradient to give the title compound (10g, 51.9% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 4.0-3.96(m,1H),3.76(s,3H),3.40-3.33(m,2H),3.18-2.99(m,7H),1.20(d,J＝6.0Hz,3H)；ES-MS：m/z 252.45(M+H)⁺。

Step 2: synthesis of methyl (2R,3S) -3- ((tert-butyldimethylsilyl) oxy) -2- (1, 1-thiomorpholino) butyrate:

to a stirred solution of methyl (2R,3S) -2- (1, 1-thiomorpholino) -3-hydroxybutyrate (step 1, 5.0g, 19.89mmol, 1.0eq) in DCM (40ml) and DMF (20ml) was added imidazole (2.7g, 39.78mmol, 2.0eq) and tert-butyldimethylchlorosilane (3.29g, 21.88mmol, 1.1eq) at 0 ℃. The reaction mixture was stirred at room temperature for about 72 hours. TLC indicated that the starting material was complete and the desired product was observed. The reaction mixture was evaporated under reduced pressure, diluted with water (200ml) and extracted with ethyl acetate (3 × 100 ml). The combined organic layers were washed with water (2 × 200ml) and Na₂SO₄Dried, filtered and concentrated under reduced pressure. The crude compound was purified by silica gel column chromatography using 0-5% methanol/dichloromethaneAn alkane gradient to give the title compound (4.5g, 61.89% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 4.41-4.37(m,1H),3.69(s,3H),3.62-3.52(m,2H),3.29-3.20(m,3H),3.11-2.99(m,4H),1.22(d,J＝6.3Hz,3H),0.82(s,9H),0.04(s,3H),0.01(s,3H)；ES-MS：m/z 366.50(M+H)⁺。

And step 3: synthesis of 4- ((2S,3S) -3- ((tert-butyldimethylsilyl) oxy) -1-hydroxybut-2-yl) thiomorpholine 1, 1-dioxide:

DIBAL-H (15.3ml, 22.97mmol, 3.0eq, 1.5M in toluene) was slowly added to a stirred solution of methyl (2R,3S) -3- ((tert-butyldimethylsilyl) oxy) -2- (1, 1-thiomorpholino) butyrate (step 2, 2.8g, 7.65mmol, 1.0eq) in DCM (56ml) at-78 ℃. The reaction mixture was stirred at-78 ℃ for about 30 minutes and at room temperature for about 1 hour. TLC indicated that the starting material was complete and the desired product was observed. The reaction mixture was quenched with aqueous ammonium chloride and extracted with DCM (3 × 80 ml). The combined organic layers were washed with water (80ml), dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude compound was purified by column chromatography on silica gel using a 0-3% methanol/dichloromethane gradient to give the title compound (1.8g, 69.7% yield) as a solid.¹H NMR(300MHz,CDCl₃)：δppm 4.02-3.92(m,1H),3.57-3.48(m,2H),3.44-3.32(m,3H),3.14-3.0(m,6H),2.43(s,1H),1.19(d,J＝6.0Hz,3H),0.88(s,9H),0.1(s,6H)；ES-MS：m/z 338.57(M+H)⁺。

Intermediate 15: preparation of (2R,3R) -3- ((tert-butyldimethylsilyl) oxy) -2- (4- (isopropylsulfonyl) Yl) piperazin-1-yl) butan-1-ol:

step 1: synthesis of O- (tert-butyldimethylsilyl) -L-threonine methyl ester:

to a stirred solution of L-threonine methyl ester hydrochloride (21.5g, 126.76mmol, 1.0q) in DCM (400ml) was added triethylamine (124.7ml, 887.32mmol, 7.0eq), 4- (dimethylamino) pyridine (1.54g, 12.676mmol, 0.1eq) and tert-butyldimethylchlorosilane (38.21g, 253.53mmol, 2.0eq) at 0 ℃. The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was diluted with water (200ml) and extracted with DCM (2 × 200 ml). The organic layer was separated, dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude compound was purified by column chromatography on silica gel using a 0-1% methanol/dichloromethane gradient to give the title compound (20g, 63.77% yield) as a colorless liquid.¹H NMR(300MHz,CDCl₃)：δppm 4.34-4.27(m,1H),3.72(s,3H),3.29(d,J＝2.7Hz,1H),1.62(brs,2H),1.26(d,J＝6.3Hz,3H),0.86(s,9H),0.04(s,6H)；ES-MS：m/z 248.37(M+H)⁺。

Step 2: synthesis of methyl (2S,3R) -2- (4-benzylpiperazin-1-yl) -3- ((tert-butyldimethylsilyl) oxy) butanoate:

to a stirred solution of O- (tert-butyldimethylsilyl) -L-threonine methyl ester (step 1, 20g, 80.83mmol, 1.0eq) and N-benzyl-2-chloro-N- (2-chloroethyl) ethan-1-amine hydrochloride (prepared as described in E.J.M.C,2014,75, pp: 11-20) (32.56g, 121.24mmol, 1.5eq) in methanol (20ml) was added N, N-diisopropylethylamine (100 ml). The reaction mixture was heated at 90-100 ℃ overnight. The reaction mixture was evaporated under reduced pressure, diluted with water (200ml) and extracted with DCM (2 × 200 ml). The organic layer was separated, dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude compound was purified by column chromatography on silica gel using 2% methanol in dichloromethane eluent to give the title compound (18g, 54.86% yield) as colorlessA liquid.¹H NMR(300MHz,CDCl₃)：δppm 7.32-7.30(m,5H),4.23-4.14(m,1H),3.67(s,3H),3.49(s,2H),3.07(d,J＝6.6Hz,1H),2.83-2.76(m,2H),2.64-2.58(m,2H),2.45(m,4H),1.19(d,J＝6.3Hz,3H),0.85(s,9H),0.04(s,6H)；APCI-MS：m/z 407.3(M+H)⁺。

And step 3: synthesis of methyl (2S,3R) -3- ((tert-butyldimethylsilyl) oxy) -2- (piperazin-1-yl) butanoate:

to a stirred suspension of 10% Pd/C (9g, 50% wet) in methanol (360ml) was added methyl (2S,3R) -2- (4-benzylpiperazin-1-yl) -3- ((tert-butyldimethylsilyl) oxy) butanoate (step 2, 18g, 44.33mmol, 1.0eq) and ammonium formate (55.90g, 886.6mmol, 20 eq). The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was filtered through a pad of celite and washed with methanol. The filtrate was evaporated under reduced pressure, diluted with DCM, washed with water, dried over sodium sulphate, filtered and evaporated under reduced pressure to give the title compound (12g, 85.7% yield) which was used as such in the next step without further purification.¹H NMR(300MHz,CDCl₃)：δppm 4.36-4.30(m,1H),3.71(s,3H),3.37-3.29(m,2H),3.23-3.11(m,5H),3.05-2.97(m,2H),1.21(d,J＝6.3Hz,3H),0.83(s,9H),0.04(s,6H)；APCI-MS：m/z 317.3(M+H)⁺。

And 4, step 4: synthesis of methyl (2S,3R) -3- ((tert-butyldimethylsilyl) oxy) -2- (4- (isopropylsulfonyl) piperazin-1-yl) butyrate:

to a stirred solution of methyl (2S,3R) -3- ((tert-butyldimethylsilyl) oxy) -2- (piperazin-1-yl) butanoate (step 3, 12.0g, 37.97mmol, 1.0eq) in DCM (180ml) was added triethylamine (21.18ml, 151.88mmol, 4.0eq) at 0 ℃, followed by isopropylsulfonyl chloride (8.12g, 56.96mmol,1.5 eq). The reaction mixture was brought to room temperature and stirred overnight. TLC indicated that the starting material was complete and the desired product was observed. The reaction mixture was diluted with water (100ml) and extracted with DCM (3 × 100 ml). The combined organic layers were washed with water (100ml), dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude compound was purified by column chromatography on silica gel using a 0-3% methanol/dichloromethane gradient to give the title compound (8.0g, 50% yield) as a liquid.¹H NMR(300MHz,CDCl₃)：δppm 4.30-4.26(m,1H),3.69(s,3H),3.34-3.31(m,4H),3.20-3.12(m,2H),3.03-2.96(m,2H),2.77-2.70(m,2H),1.34(d,J＝6.9Hz,6H),1.22(d,J＝6.3Hz,3H),0.85(s,9H),0.05(s,3H),0.02(s,3H)；ES-MS：m/z 423.1(M+H)⁺。

And 5: synthesis of (2R,3R) -3- ((tert-butyldimethylsilyl) oxy) -2- (4- (isopropylsulfonyl) piperazin-1-yl) butan-1-ol:

to a stirred solution of methyl (2S,3R) -3- ((tert-butyldimethylsilyl) oxy) -2- (4- (isopropylsulfonyl) piperazin-1-yl) butanoate (step 4,5.0 g, 11.82mmol, 1.0eq) in DCM (75ml) was added DIBAL-H (19.7ml, 29.57mmol, 2.5eq, 1.5M in toluene) at-78 ℃. The reaction mixture was stirred at-78 ℃ for about 4 hours. TLC indicated that the starting material was complete and the desired product was observed. The reaction mixture was quenched with saturated ammonium chloride solution and stirred for about 10 minutes. The mixture was filtered and the filtrate was extracted with DCM (3 × 100 ml). The combined organic layers were washed with water (100ml), dried over sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified by column chromatography on silica gel using a 0-3% methanol/dichloromethane gradient to give the title compound (3.7g, 80% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 3.99-3.95(m,1H),3.54-3.26(m,6H),3.22-3.13(m,1H),3.04-2.96(m,2H),2.82-2.75(m,2H),2.63-2.56(m,1H),1.34(d,J＝6.9Hz,6H),1.15(d,J＝6.3Hz,3H),0.89(s,9H),0.086(s,3H),0.081(s,3H)；ES-MS：m/z 395.46(M+H)⁺。

Intermediate 16:preparation of (1S,3R) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1- ((1R,3aS,5aR,5bR, 7aR,9S,11aR,11bR,13aR,13bR) -3 a-amino-5 a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosaenoic acid hydro-1H-cyclopenta [ a] -9-yl) -3-benzyl ester:

step 1: synthesis of (1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3 a-isocyanato-5 a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-hydro-1H-cyclopenta [ a ] a]-9-yl acetate:

to (1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -9-acetoxy-5 a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-hydro-3 aH-cyclopenta [ a-a ] at 0 deg.C]To a stirred solution of (1-intermediate 1-step 1, 26g, 52.13mmol, 1.0eq) in toluene was added diphenyl phosphorazidate (28.684g, 104.26mmol, 2.0eq) and triethylamine (15.82g, 156.39mmol, 3.0 eq). The reaction mixture was heated to 80 ℃ for 1.5 hours. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was evaporated under reduced pressure, diluted with water (500ml) and extracted with DCM (3 × 500 ml). The combined organic layers were washed with water (200ml) and brine solution (200 ml). The organic layer was dried over sodium sulfate, filtered and evaporated under reduced pressure. The crude compound was treated with methanol (78ml), stirred at room temperature for 15 minutes, filtered and dried under vacuum to give the title compound (23g,89.1% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 4.74(d,J＝1.5Hz,1H),4.63(t,J＝1.5Hz,1H),4.45(m,1H),2.57-2.50(m,1H),2.15-2.02(m,1H),2.04(s,3H),1.88-0.77(m,23H),1.68(s,3H),1.05(s,3H),0.93(s,3H),0.86(s,3H),0.84(s,3H),0.84(s,3H)。

Step 2: synthesis of (1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3 a-amino-5 a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-hydro-1H-cyclopenta [ a]-9-alkoxide:

to (1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3 a-isocyanato-5 a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosahydro-1H-cyclopenta [ a ] at 0 deg.C]To a stirred solution of (e) -9-yl acetate (step 1, 23g, 46.394mmol, 1.0eq) in 1, 4-dioxane (230ml) was added concentrated hydrochloric acid (69 ml). The reaction mixture was heated at 60 ℃ overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was concentrated under reduced pressure. The resulting compound was treated with heptane (69ml), stirred for 15 minutes, the solid filtered and dried in vacuo to give the title compound (20g, 93% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 7.67(s,3H),4.72(s,1H),4.62(s,1H),3.09-2.94(m,1H),2.60(m,1H),2.11-1.99(m,1H),1.91-1.79(m,2H),1.76-0.62(m,21H),1.66(s,3H),1.03(s,3H),0.93(s,3H),0.87(s,3H),0.78(s,3H),0.65(s,3H)。

And step 3: synthesis of ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -9-hydroxy-5 a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-hydro-3 aH-cyclopenta [ a]-3 a-yl)Tert-butyl carbamate:

to (1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3 a-amino-5 a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-hydro-1H-cyclopenta [ a-a ] at 0 deg.C]To a stirred solution of-9-alkoxide (step 2, 20g, 43.087mmol, 1.0eq) in 1, 4-dioxane (200ml) was added a saturated solution of sodium bicarbonate (200ml) and di-tert-butyl dicarbonate (14.1g, 64.631mmol, 1.5 eq). The reaction mixture was stirred at room temperature for 6 hours. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was diluted with water (400ml) and extracted with DCM (3 × 500 ml). The combined organic layers were washed with water (400ml) and brine solution (200 ml). The organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude compound was purified by silica gel column chromatography using a 0-3% ethyl acetate/hexane gradient. Fractions containing the expected product were combined and concentrated under reduced pressure to give the title compound (16g, 70.48% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 4.732(d,J＝1.5Hz,1H),4.628(d,J＝1.5Hz,1H),4.33(s,1H),3.22-3.17(m,1H),2.58-2.54(m,1H),2.50-2.35(m,2H),2.06-1.92(m,1H),1.70(s,3H),1.66-0.69(m,21H),1.46(s,9H),1.03(s,3H),0.99(s,3H),0.97(s,3H),0.85(s,3H),0.78(s,3H)；ESI-MS：m/z 550.45(M+Na)⁺。

And 4, step 4: synthesis of (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- ((tert-butoxycarbonyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-hydro-1H-cyclopenta [ a]-9-yl) ester:

to ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -9-hydroxy-5 a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-hydro-3 aH-cyclopenta [ a-a ] at 0 ℃]Tert-butyl (3 a-yl) carbamate (step 3, 16g, 30.312mmol, 1.0eq) to a stirred solution in DCM (240ml) was added (1S,3R) -3- ((benzyloxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid (prepared as described in WO2011/007230a2, 11.92g, 45.469mmol, 1.5eq), 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride (edc.hcl) (14.52g, 75.781mmol, 2.5eq) and 4- (dimethylamino) pyridine (1.11g, 9.093mmol, 0.3 eq). The reaction mixture was stirred at room temperature overnight. TLC indicated that the starting material was complete and the desired product was observed. The reaction mixture was diluted with water (500ml) and extracted with DCM (3 × 500 ml). The combined organic layers were washed with water (200ml) and brine solution (100 ml). The organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude compound was purified by silica gel column chromatography using a 0-3% ethyl acetate/hexane gradient. The fractions containing the expected product were combined and concentrated under reduced pressure to give the title compound (16g, 68.37% yield) as an off-white solid. 1H NMR (300MHz, CDCl)₃)：δppm 7.36-7.32(m,5H),5.14,5.09(ABq,J_AB＝12.3Hz,2H),4.70(s,1H),4.60(s,1H),4.43(dd,J＝11.4,4.5Hz,1H),4.30(s,1H),2.84-2.51(m,4H),2.48-2.31(m,2H),2.07-0.76(m,23H),1.68(s,3H),1.43(s,9H),1.34(s,3H),1.01(s,3H),0.96(s,3H),0.95(s,3H),0.85(s,6H),0.84(s,3H)；ESI-MS：m/z 794.55(M+Na)⁺。

And 5: synthesis of (1S,3R) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3 a-amino-5 a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-hydro-1H-cyclopenta [ a]9-yl) -3-benzyl ester:

to (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- ((tert-butoxycarbonyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosahydro-1H-cyclopenta [ a]-9-yl) ester (step 4, 11.0g, 14.24mmol, 1.0eq) to a stirred solution in 1, 4-dioxane (55ml) was added 4N HCl in 1, 4-dioxane (55 ml). The reaction mixture was brought to room temperature and stirred for about 3 hours. TLC indicated that the starting material was complete and the desired product was observed. The reaction mixture was evaporated under reduced pressure, diluted with water (50ml), basified with aqueous sodium bicarbonate and extracted with DCM (3 × 100 ml). The combined organic layers were washed with water (100ml), dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude compound was treated with hexane (50ml), stirred, filtered and dried in vacuo to give the title compound (7.0g, 73.1% yield) as a solid. 1H NMR (300MHz, CDCl 3): delta ppm 7.36-7.32(m,5H),5.14,5.09(ABq, J)_AB＝12.3Hz,2H),4.72(s,1H),4.59(s,1H),4.43(dd,J＝11.4,4.5Hz,1H),2.84-2.48(m,4H),2.07-1.98(m,2H),1.68(s,3H),1.63-0.76(m,23H),1.34(s,3H),1.04(s,3H),0.96(s,3H),0.95(s,3H),0.85(s,6H),0.83(s,3H)；ES-MS：m/z 672.49(M+H)⁺。

Intermediate 17: preparation of 2- (2-methoxyethoxy) acetic acid:

step 1: synthesis of ethyl 2- (2-methoxyethoxy) acetate:

to a stirred solution of sodium hydride (6.6g, 275.98mmol, 3.0eq) in THF (140ml) was added 2-methoxyethon-1-ol (7.0g, 91.996mmol, 1.0eq) at 0 ℃. The reaction mixture was stirred at 0 ℃ for about 30 minutes. Ethyl bromoacetate (19.97g, 119.59mmol, 1.3eq) and potassium iodide (3.054g, 18.399mmol, 0.2eq) were added to the reaction mixture and stirred at room temperature for about 2 hours. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was cooled to 0 ℃, quenched with saturated sodium chloride solution and extracted with DCM (3 × 350 ml). The combined organic layers were washed with water (150ml), dried over sodium sulfate, filtered and concentrated under reduced pressure to give the title compound (10g, 67% yield) as an oil which was used in the next step without further purification.

Step 2: synthesis of 2- (2-methoxyethoxy) acetic acid:

to a stirred solution of ethyl 2- (2-methoxyethoxy) acetate (step 1, 10g, 61.656mmol, 1.0eq) in methanol (100ml) and THF (100ml) was added 2.5N aqueous KOH (184.96ml, 462.42mmol, 7.5eq) at 0 ℃. The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was evaporated under reduced pressure, cooled to 0 ℃, adjusted to pH 2.0 with 1N HCl and evaporated under reduced pressure. The resulting solid was stirred with DCM (4 × 150ml), filtered and the filtrate evaporated under reduced pressure to give the title compound (5.0g, 60.97% yield) as an oil.¹H NMR(300MHz,CDCl₃)：δppm 4.19(s,2H),3.77-3.74(m,2H),3.64-3.59(m,2H),3.42(s,3H)；ES-MS：m/z 135.17(M+H)⁺。

Intermediate 18: preparation of 4- ((12R,13R) -14-hydroxy-12-methyl-2, 5,8, 11-tetraoxatetradecane-13- Base) thiomorpholine 1, 1-dioxide:

step 1: synthesis of 2- (2- (2-methoxyethoxy) ethoxy) ethyl methanesulfonate:

to a stirred solution of 2- (2- (2-methoxyethoxy) ethoxy) ethan-1-ol (3.29g, 20.03mmol, 1.0eq) in DCM (40ml) was added triethylamine (8.4ml, 60.09mmol, 3.0eq) followed by methanesulfonyl chloride (2.01ml, 26.04mmol, 1.3eq) at 0 ℃. The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. Water was added to the reaction mixture and extracted with DCM (2 × 30 ml). The combined organic layers were dried over sodium sulfate, filtered and concentrated under reduced pressure to give the title compound (4.3g) as a liquid, which was used in the next step without further purification.¹H NMR(300MHz,CDCl₃)：δppm 4.40-4.36(m,2H),3.78-3.75(m,2H),3.70-3.61(m,6H),3.56-3.52(m,2H),3.37(s,3H),3.07(s,3H)；ES-MS：m/z 243.37(M+H)⁺。

Step 2: synthesis of 4- ((2R,3R) -1, 3-dihydroxybutan-2-yl) thiomorpholine 1, 1-dioxide:

to a stirred solution of methyl (2S,3R) -2- (1, 1-thiomorpholino) -3-hydroxybutyrate (intermediate 8-step 2, 15g, 59.713mmol, 1.0eq) in methanol (300ml) was added sodium borohydride (22.5g, 597.13mmol, 10.0eq) portionwise at 0 ℃. The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was neutralized with 4N HCl/1, 4-dioxane (120ml) and concentrated under reduced pressure. The residue was stirred with DCM, filtered and the filtrate evaporated under reduced pressure. The crude product was purified by silica gel column chromatography using a 0-5% methanol/dichloromethane gradient to give the title compound (6.5g, 48.8% yield) as an off-white solid.¹H NMR(300MHz,DMSO-d₆)：δppm 4.38(t,J＝4.8Hz,1H),4.26(d,J＝3.0Hz,1H),4.09-4.04(m,1H),3.61-3.44(m,2H),3.25-3.16(m,2H),3.08-3.02(m,6H),2.40-2.33(m,1H),1.04(d,J＝6.0Hz,3H)；ES-MS：m/z 224.16(M+H)⁺。

And step 3: synthesis of 4- ((2R,3R) -1- ((tert-butyldimethylsilyl) oxy) -3-hydroxybut-2-yl) thiomorpholine 1, 1-dioxide:

to a stirred solution of 4- ((2R,3R) -1, 3-dihydroxybutan-2-yl) thiomorpholine 1, 1-dioxide (step 2, 4.5g, 20.153mmol, 1.0eq) in DCM (90ml) was added triethylamine (14ml, 100.76mmol, 5.0eq), DMAP (0.492g, 4.030mmol, 0.2eq) and DMSCl (7.59g, 50.383mmol, 2.5eq) at 0 ℃. The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was diluted with DCM (200ml) and washed with water (200ml) and brine solution (250 ml). The organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude product was purified by silica gel column chromatography using a gradient of 0-60% ethyl acetate in hexanes to give the title compound (4g, 58.8% yield) as a white solid.¹H NMR(300MHz,CDCl₃)：δppm 3.80(dd,J＝11.1,3.0Hz,1H),3.72-3.61(m,2H),3.52-3.39(m,3H),3.12-3.02(m,6H),2.50-2.43(m,1H),1.20(d,J＝6.0Hz,3H),0.89(s,9H),0.07(s,6H)；ES-MS：m/z 338.32(M+H)⁺。

And 4, step 4: synthesis of 4- ((12R,13R) -12,16,16,17, 17-pentamethyl-2, 5,8,11, 15-pentaoxa-16-silaoctadecan-13-yl) thiomorpholine 1, 1-dioxide:

to a suspension of sodium hydride (1.77g, 74.05mmol, 5.0eq) in THF (50ml) was added 4- ((2R,3R) -1- ((tert-butyldimethylsilyl) oxy) -3-hydroxybut-2-yl) thiomorpholine 1, 1-dioxide (step 3, 5g, 14.81mmol, 1.0eq) at 0 ℃. The reaction mixture was stirred at 40-50 ℃ for about 30 minutes. 2- (2- (2-methoxyethoxy) ethoxy) ethyl methanesulfonate (step 1, 4.3g, 17.77mmol, 1.2eq) and potassium iodide (0.245g, 1.48mmol, 0.1eq) were added to the reactionThe mixture was allowed to stir at 50-60 ℃ overnight. The reaction mixture was cooled to 0 ℃, quenched with ice-cold water and extracted with DCM (2 × 50 ml). The combined organic layers were dried over sodium sulfate, filtered and concentrated under reduced pressure. The product was purified by silica gel column chromatography using a 0-40% ethyl acetate/hexanes gradient to give the title compound (2.0g, 27.93% yield) as a colorless liquid.¹H NMR(300MHz,CDCl₃)：δppm 4.0-3.93(m,1H),3.84-3.70(m,2H),3.68-3.63(m,10H),3.62-3.50(m,4H),3.38(s,3H),3.14-2.95(m,6H),2.66-2.61(m,1H),1.20(d,J＝6.6Hz,3H),0.89(s,9H),0.07(s,3H),0.05(s,3H)；ES-MS：m/z 484.65(M+H)⁺。

And 5: synthesis of 4- ((12R,13R) -14-hydroxy-12-methyl-2, 5,8, 11-tetraoxatetradec-13-yl) thiomorpholine 1, 1-dioxide:

to a stirred solution of 4- ((12R,13R) -12,16,16,17, 17-pentamethyl-2, 5,8,11, 15-pentaoxa-16-silaoctadeca-13-yl) thiomorpholine 1, 1-dioxide (step 4, 2.0g, 4.134mmol, 1.0eq) in THF (20ml) was added TBAF (8.26ml, 8.269mmol, 2.0eq, 1.0M in THF). The reaction mixture was stirred at room temperature overnight. TLC indicated that the starting material was complete and the desired product was observed. The reaction mixture was diluted with DCM (100ml) and washed with water (100 ml). The organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude product was purified by silica gel column chromatography using a 0-9% methanol/dichloromethane gradient to give the title compound (0.8g, 52.6% yield) as a colorless liquid.¹H NMR(300MHz,CDCl₃)：δppm 3.67-3.59(m,9H),3.58-3.53(m,4H),3.47-3.41(m,2H),3.38(s,3H),3.35-3.20(m,2H),3.13-3.0(m,6H),2.63-2.56(m,1H),1.20(d,J＝6.0Hz,3H)；ES-MS：m/z 370.20(M+H)⁺。

Intermediate 19: preparation of 2,5,8, 11-tetraoxatridec-13-oic acid:

step 1: synthesis of ethyl 2,5,8, 11-tetraoxatridec-13-oate:

to a stirred suspension of sodium hydride (3.65g, 152.25mmol, 5.0eq) in THF (125ml) was added 2- (2- (2-methoxyethoxy) ethoxy) ethan-1-ol (5.0g, 30.45mmol, 1.0eq) and tetrabutylammonium iodide (2.24g, 6.09mmol, 0.2eq) at 0 ℃. The reaction mixture was stirred at 0 ℃ for about 30 minutes, ethyl 2-bromoacetate (6.611g, 39.58mmol, 1.3eq) was added, stirred at 0 ℃ for about 1 hour and stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was cooled to 0 ℃, quenched with ice and extracted with DCM (3 × 250 ml). The combined organic layers were washed with water (150ml) and brine solution (150 ml). The organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure to give the title compound as a solid (6g, 78.74% yield) which was used as such in the next step without further purification.

Step 2: synthesis of 2,5,8, 11-Tetraoxatridec-13-oic acid:

to a stirred solution of ethyl 2,5,8, 11-tetraoxatridec-13-oate (step 1, 6.0g, 23.97mmol, 1.0eq) in methanol (60ml) and THF (60ml) was added 2.5N aqueous KOH (71.85ml, 179.79mmol, 7.5eq) at 0 ℃. The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was evaporated under reduced pressure, water (50ml) was added, cooled to 0 ℃, acidified to pH 2.0 with 1N HCl and extracted with DCM (3 × 250 ml). The combined organic layers were washed with water (100ml), dried over sodium sulfate, filtered and concentrated under reduced pressure. The residue (5.3g) was used as such in the next step without further purification.¹H NMR(300MHz,CDCl₃)：δppm4.17(s,2H),3.75-3.57(m,12H),3.39(s,3H)；ES-MS：m/z 223.63(M+H)⁺。

Examples

Example 1: preparation of (1R,3S) -3- (((((1R, 3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) - 3a- ((2- (1, 1-thiomorpholino) -2-methylpropyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methyl) Cyclopropyl) eicosahydro-1H-cyclopenta [ a] -9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid:

step 1: synthesis of (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- ((2- ((tert-butoxycarbonyl) amino) -2-methylpropyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-hydro-1H-cyclopenta [ a]-9-yl) ester:

to (1S,3R) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3 a-amino-5 a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]To a stirred solution of-9-yl) -3-benzyl ester (intermediate 1, 4g, 5.83mmol, 1.0eq) in 1, 2-dichloroethane (40ml) were added tert-butyl (2-methyl-1-oxoprop-2-yl) carbamate (intermediate 2, 3.27g, 17.49mmol, 3.0eq) and titanium tetraisopropoxide (3.4ml, 11.66mmol, 2.0 eq). The reaction mixture was stirred at room temperature for about 1 hour, and then triacetoxyboron was addedSodium hydride (3.7g, 17.49mmol, 3.0eq) and stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. Saturated sodium bicarbonate solution was added to the reaction mixture, the organic layer was separated and the aqueous layer was extracted with dichloromethane (2 × 40 ml). The combined organic layers were washed with water and Na₂SO₄Dried, filtered and concentrated under reduced pressure. The crude compound was purified by silica gel column chromatography eluting with 1% methanol in dichloromethane to give the title compound (4g, 80% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 7.34(m,5H),5.15,5.09(ABq,J_AB＝12.3Hz,2H),4.45(m,1H),2.90-2.46(m,3H),2.40-2.30(m,2H),2.10-2.0(m,2H),1.97-1.13(m,32H),1.42(s,9H),1.03-0.76(m,22H),0.40-0.20(m,4H)；ESI-MS：m/z 857.75(M+H)⁺。

Step 2: synthesis of (1S,3R) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- ((2-amino-2-methylpropyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-hydro-1H-cyclopenta [ a ] a]-9-yl) -3-benzyl ester:

to (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- ((2- ((tert-butoxycarbonyl) amino) -2-methylpropyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a [ a ] at 0 deg.C]To a stirred solution of (9-yl) ester (step 1, 4g, 4.66mmol, 1.0eq) in 1, 4-dioxane (10ml) was added 4N HCl/1, 4-dioxane (30 ml). The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was evaporated under reduced pressure, basified with saturated sodium bicarbonate solution and dichloromethane(2 × 40 ml). The organic layer was washed with water, Na₂SO₄Drying, filtration and concentration under reduced pressure gave the title compound (3g, 85.2% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 7.34(m,5H),5.14,5.09(ABq,J_AB＝12.3Hz,2H),4.45(m,1H),2.84-2.61(m,3H),2.27-2.13(m,2H),2.07-1.17(m,34H),1.13-0.78(m,22H),0.36-0.22(m,4H)；ESI-MS：m/z 757.50(M+H)⁺。

And step 3: synthesis of (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- ((2- (1, 1-thiomorpholino) -2-methylpropyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-hydro-1H-cyclopenta [ a]9-yl) ester:

to (1S,3R) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- ((2-amino-2-methylpropyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) · 3-benzyl ester (step 2, 0.4g, 0.52mmol, 1.0eq) to a stirred solution in 1, 4-dioxane (4ml) and ethanol (4ml) was added triethylamine (0.21ml, 1.56mmol, 3.0eq) followed by divinyl sulfone (0.12g, 1.056mmol, 2.0 eq). The reaction mixture was heated to 85 ℃ for about 3 hours. TLC indicated that the starting material was complete and the desired product was observed. The reaction mixture was evaporated under reduced pressure, diluted with water (100ml) and extracted with ethyl acetate (3 × 30 ml). The combined organic layers were washed with water (100ml) and Na₂SO₄Dried, filtered and concentrated under reduced pressure. The residue was purified by column chromatography on silica gel using a gradient of 20-40% ethyl acetate in hexanes to give the title compound (0.4g, 88.8% yield) as a solid.¹H NMR(300MHz,CDCl₃)：δppm 7.35(m,5H),5.14,5.09(ABq,J_AB＝12.3Hz,2H),4.49-4.40(m,1H),3.05(m,8H),2.86-2.73(m,2H),2.71-2.60(m,1H),2.50-2.28(m,2H),2.10-1.13(m,34H),1.08-0.78(m,22H),0.42-0.22(m,4H)。

And 4, step 4: synthesis of (1R,3S) -3- (((((1R, 3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- ((2- (1, 1-thiomorpholino) -2-methylpropyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-hydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid:

to (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- ((2- (1, 1-thiomorpholino) -2-methylpropyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a]To a stirred solution of the-9-yl) ester (step 3, 0.4g, 0.456mmol, 1.0eq) in THF (4ml) and methanol (4ml) was added 2.5N aqueous KOH (1.35ml, 3.427mmol, 7.5 eq). The reaction mixture was stirred at room temperature overnight. TLC indicated that the starting material was complete and the desired product was observed. The reaction mixture was evaporated under reduced pressure and diluted with water (5 ml). The reaction mixture was cooled to 0 ℃, the pH was adjusted to 5 with 1N HCl and extracted with dichloromethane (3 × 30 ml). The combined organic layers were washed with water (20ml) and brine solution (10 ml). Na for organic layer₂SO₄Dried, filtered and concentrated under reduced pressure. The crude compound was purified by silica gel column chromatography using a 30 to 45% ethyl acetate/hexanes gradient to give the title compound (0.15g, 41.8% yield) as a white solid.¹H NMR(300MHz,CDCl₃)：δppm 4.46(dd,J＝10.8,4.8Hz,1H),3.10-2.92(m,8H),2.86-2.74(m,2H),2.65-2.53(m,1H),2.39-2.28(m,2H),2.10-2.0(m,2H),1.98-1.05(m,32H),1.03-0.78(m,22H),0.40-0.20(m,4H)。ESI-MS：m/z 785.65(M+H)⁺。

Example 2: preparation of (1R,3S) -3- (((((1R, 3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) - 3a- (((S) -2- (1, 1-thiomorpholino) propyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclo-cyclo Propyl) eicosahydro-1H-cyclopenta [ a] -9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid:

step 1: synthesis of (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((S) -2- (1, 1-thiomorpholino) propyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-hydro-1H-cyclopenta [ a ] a]9-yl) ester:

to (1S,3R) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3 a-amino-5 a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a](S) -4- (1-Chloropropane-2-yl) thiomorpholine 1, 1-dioxide (intermediate 3, 0.925g, 4.372mmol, 3.0eq), tripotassium phosphate (1.36g, 6.4108mmol, 4.4eq) and iodinated Potassium (1.36g, 6.4108mmol, 4.4eq) were added to a stirred solution of-9-yl). 3-benzyl ester (intermediate 1, 1g, 1.457mmol, 1.0eq) in acetonitrile (20ml)Potassium (0.653g, 3.934mmol, 2.7 eq). The reaction mixture was heated to reflux overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was filtered through a pad of celite and washed with acetonitrile (25 ml). The filtrate was evaporated under reduced pressure and the residue was purified by column chromatography on silica gel using 2% methanol/dichloromethane as eluent to give the title compound (0.35g, 27.88% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 7.35(m,5H),5.15,5.09(ABq,J_AB＝12.3Hz,2H),4.44(dd,J＝10.5,4.8Hz,1H),3.32(m,1H),3.12-3.06(m,8H),2.88-2.72(m,2H),2.70-2.52(m,2H),2.50-2.48(m,1H),2.18-2.0(m,3H),1.92-1.17(m,25H),1.17-0.78(m,25H),0.68(m,1H),0.50-0.36(m,2H),0.28(m,1H)；ES-MS：m/z 861.79(M+H)⁺。

Step 2: synthesis of (1R,3S) -3- (((((1R, 3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((S) -2- (1, 1-thiomorpholino) propyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid:

to (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((S) -2- (1, 1-thiomorpholino) propyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a at 0 deg.C]To a stirred solution of (9-yl) ester (step 1, 0.35g, 0.406mmol, 1.0eq) in THF (3.5ml) and methanol (3.5ml) was added 2.5N aqueous KOH (1.22ml, 3.047mmol, 7.5 eq). The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was evaporated under reduced pressure, diluted with water (10ml), cooled to 0 deg.C,and the pH was adjusted to 5.0 with 1N HCl. The aqueous layer was extracted with dichloromethane (2 × 20 ml). The combined organic layers were washed with water and Na₂SO₄Dried, filtered and concentrated under reduced pressure. The crude compound was purified by silica gel column chromatography using a 0-5% methanol/DCM gradient. The resulting compound was further purified by recrystallization from MTBE (10ml) to give the title compound (0.1g, 32% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 4.46(m,1H),3.30-2.90(m,9H),2.88-2.72(m,2H),2.68-2.50(m,2H),2.28-2.0(m,3H),2.0-1.17(m,26H),1.14-0.78(m,25H),0.62-0.52(m,1H),0.50-0.36(m,2H),0.32-0.25(m,1H)；ES-MS：m/z 771.87(M+H)⁺。

Example 3: preparation of (1R,3S) -3- (((((1R, 3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) - 3a- (((R) -2- (1, 1-thiomorpholino) -3-methylbutyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1- Methylcyclopropyl) eicosahydro-1H-cyclopenta [ a] -9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-methyl Acid:

step 1: synthesis of (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((R) -2- (1, 1-thiomorpholino) -3-methylbutyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-hydro-1H-cyclopenta [ a ] a]-9-yl) ester:

at 0To a stirred solution of (R) -4- (1-hydroxy-3-methylbutan-2-yl) thiomorpholine 1, 1-dioxide (intermediate 4, 0.241g, 1.093mmol, 1.25eq) in dichloromethane (12ml) was added triflic anhydride (0.20ml, 1.224mmol, 1.4 eq). The reaction mixture was stirred at the same temperature for about 10 minutes. 2, 6-lutidine (0.145ml, 1.25mmol, 1.43eq) was added and stirred at 0 ℃ for 10 min. To the reaction mixture was added (1S,3R) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3 a-amino-5 a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl). 3-benzyl ester (intermediate 1, 0.6g, 0.874mmol, 1.0), then triethylamine (0.175ml, 1.25mmol, 1.43eq) is added and stirred at 0 ℃ for about 1 hour. The cooling bath was removed and stirred at room temperature for about 4 hours. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was diluted with dichloromethane (2 × 20ml) and the organic layer was washed with water, dried over sodium sulfate, filtered and evaporated under reduced pressure. The crude compound was purified by column chromatography on silica gel using 30% ethyl acetate/hexane as eluent to give the title compound (0.3g, 38.96% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm7.35(m,5H),5.15,5.09(ABq,J_AB＝12.3Hz,2H),4.45(dd,J＝11.1,4.5Hz,1H),3.22-3.06(m,6H),3.02-2.94(m,2H),2.88-2.58(m,3H),2.52-2.18(m,3H),2.10-1.90(m,2H),1.88-1.10(m,27H),1.06-0.78(m,28H),0.40-0.20(m,4H)；ES-MS：m/z 889.81(M+H)⁺。

Step 2: synthesis of (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((R) -2- (1, 1-thiomorpholino) -3-methylbutyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-hydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid:

to (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((R) -2- (1, 1-thiomorpholino) -3-methylbutyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) ester (step 1, 0.3g, 0.337mmol, 1.0eq) to a stirred solution in methanol (3ml) and THF (3ml) was added 2.5N aqueous KOH (1.01ml, 2.527mmol, 7.5 eq). The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was evaporated under reduced pressure, cooled to 0 ℃, acidified to pH 5.0 with 1N HCl and extracted with dichloromethane (2 × 30 ml). The combined organic layers were washed with water and Na₂SO₄Dried, filtered and concentrated under reduced pressure. The crude compound was purified by column chromatography on silica gel using 5% methanol in dichloromethane as eluent to give the title compound (0.100g, 37.3% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 4.45(m,1H),3.25-2.95(m,8H),2.80-2.70(m,3H),2.62-2.42(m,2H),2.38-2.20(m,1H),2.20-1.20(m,29H),1.18-0.78(m,28H),0.42-0.20(m,4H)；ES-MS：m/z 799.89(M+H)⁺。

Example 4: preparation of (1R,3S) -3- (((((1R, 3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) - 3a- (((R) -3- ((tert-butyldimethylsilyl) oxy) -2- (1, 1-thiomorpholino) propyl) amino) - 5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a] -9-yl) oxy) carbonyl Yl) -2, 2-dimethylcyclobutane-1-carboxylic acid:

step 1: synthesis of (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((R) -3- ((tert-butyldimethylsilyl) oxy) -2- (1, 1-thiomorpholino) propyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) ester:

to a stirred solution of (R) -4- (1- ((tert-butyldimethylsilyl) oxy) -3-hydroxypropan-2-yl) thiomorpholine 1, 1-dioxide (intermediate 6, 0.742g, 2.186mmol, 1.5eq) in DCM (20ml) was added triflic anhydride (0.39ml, 2.332mmol, 1.6eq) at 0 ℃. The reaction mixture was stirred at 0 ℃ for about 10 minutes, then 2, 6-lutidine (0.21ml, 2.332mmol, 1.6eq) was added and stirred at 0 ℃ for about 10 minutes. (1S,3R) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3 a-amino-5 a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a-at 0 ℃ in succession]-9-yl). 3-benzyl ester (intermediate 1, 1g, 1.457mmol, 1.0eq) and triethylamine (0.32ml, 2.332mmol, 1.6eq) and stirred at the same temperature for about 1 hour. The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was diluted with DCM (100ml) and washed with saturated sodium bicarbonate solution, water and brine solution. Na for organic layer₂SO₄Dried, filtered and evaporated under reduced pressure. The crude compound was purified by column chromatography on silica gel using a 0-15% ethyl acetate/hexanes gradient to give the title compound (1g, 69.4% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 7.34(m,5H),5.14,5.09(ABq,J_AB＝12.3Hz,2H),4.44(dd,J＝11.1,4.8Hz,1H),3.71-3.65(m,2H),3.40-3.20(m,3H),3.19-2.98(m,8H),2.85-2.57(m,3H),2.46-2.40(m,2H),2.08-2.0(m,1H),1.95-0.70(m,26H),1.34(s,3H),1.05(s,3H),0.96(s,3H),0.898(s,6H),0.891(s,6H),0.84(s,9H),0.36-0.21(m,4H),0.07(s,3H),0.05(s,3H)；ESI-MS：m/z 992.16(M+H)⁺。

Step 2: synthesis of (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((R) -3- ((tert-butyldimethylsilyl) oxy) -2- (1, 1-thiomorpholino) propyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-hydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid:

to (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((R) -3- ((tert-butyldimethylsilyl) oxy) -2- (1, 1-thiomorpholino) propyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) ester (step 1, 1g, 1.008mmol, 1.0eq) to a stirred solution in methanol (10ml) and THF (10ml) was added 2.5N aqueous KOH (3ml, 7.56mmol, 7.5 eq). The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was evaporated under reduced pressure, cooled to 0 ℃, adjusted to pH 5.0 with 1N HCl, and extracted with DCM (2 × 100 ml). The combined organic layers were washed with water (100ml) and brine solution (100 ml). Na for organic layer₂SO₄Dried, filtered and evaporated under reduced pressure. The crude compound was purified by column chromatography on silica gel using a 0 to 6% methanol/dichloromethane gradient to give the title compound (0.350g, 38.5% yield)As an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 4.53-4.45(m,1H),3.76-3.62(m,2H),3.40-3.23(m,3H),3.20-2.93(m,8H),2.80-2.52(m,5H),2.02-0.78(m,24H),1.35(s,3H),1.05(s,3H),1.0(s,3H),0.98(s,3H),0.89(s,3H),0.88(s,3H),0.87(s,3H),0.85(s,3H),0.83(s,9H),0.40-0.24(m,4H),0.05(s,3H),0.04(s,3H)；ESI-MS：m/z 901.91(M+H)⁺。

Example 5: preparation of (1R,3S) -3- (((((1R, 3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) - 3a- (((R) -2- (1, 1-thiomorpholino) -3-hydroxypropyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1- Methylcyclopropyl) eicosahydro-1H-cyclopenta [ a] -9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid:

to (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((R) -3- ((tert-butyldimethylsilyl) oxy) -2- (1, 1-thiomorpholino) propyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] at 0 deg.C]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid (example 4, 0.500g, 0.554mmol, 1.0eq) to a stirred solution in THF (15ml) was added TBAF (0.66ml, 0.6659mmol, 1.2eq, 1.0M in THF). The reaction mixture was stirred at room temperature for about 4 hours. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was evaporated under reduced pressure. The residue was dissolved in ethyl acetate (100ml) and washed with water (2 × 100ml) and brine solution. Na for organic layer₂SO₄Dried, filtered and concentrated under reduced pressure. Purifying the crude compound by silica gel column chromatography with a gradient of 0-10% methanol/dichloromethane to obtainTo the title compound (0.110g, 25.2% yield) as an off-white solid.¹H NMR(300MHz,DMSO-d6)：δppm 4.35(m,1H),3.55-3.45(m,1H),3.44-3.38(m,1H),3.20-3.10(m,2H),3.09-2.92(m,6H),2.82-2.70(m,3H),2.35-2.22(m,4H),1.98-0.78(m,25H),1.33(s,3H),1.02(s,3H),0.95(s,3H),0.91(s,3H),0.89(s,3H),0.83(s,6H),0.82(s,3H),0.35-0.20(m,4H)；ESI-MS：m/z 787.55(M+H)⁺。

Example 6: preparation of (1R,3S) -3- (((((1R, 3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) - 3a- (((S) -2- (1, 1-thiomorpholino) -3-methylbutyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1- Methylcyclopropyl) eicosahydro-1H-cyclopenta [ a] -9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid:

step 1: synthesis of (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((S) -2- (1, 1-thiomorpholino) -3-methylbutyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-hydro-1H-cyclopenta [ a ] a]-9-yl) ester:

to a stirred solution of (S) -4- (1-hydroxy-3-methylbutan-2-yl) thiomorpholine 1, 1-dioxide (intermediate 7, 0.290g, 1.311mmol, 1.5eq) in DCM (10ml) was added triflic anhydride (0.234ml, 1.399mmol, 1.6eq) at 0 ℃. The reaction mixture was stirred at 0 ℃ for about 10 minutes. 2, 6-lutidine (0.161ml, 1.399 mm) was addedol, 1.6eq) and stirred at 0 ℃ for 10 minutes. (1S,3R) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3 a-amino-5 a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a-at 0 ℃ in succession]-9-yl). 3-benzyl ester (intermediate 1, 0.6g, 0.8745mmol, 1.0eq) and triethylamine (0.36ml, 2.623mmol, 3.0eq) and stirred at room temperature for about 4 hours. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was quenched with saturated sodium bicarbonate solution and extracted with DCM (2 × 20 ml). The combined organic layers were washed with water and Na₂SO₄Dried, filtered and concentrated under reduced pressure. The crude compound was purified by column chromatography on silica gel using 2% methanol in DCM as eluent to give the title compound (0.4g, 51.48% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 7.36-7.31(m,5H),5.15,5.09(ABq,J_AB＝12.3Hz,2H),4.44(dd,J＝11.1,4.8Hz,1H),3.15-3.13(m,4H),3.03-2.98(m,4H),2.84-2.73(m,2H),2.70-2.59(m,1H),2.50-2.37(m,2H),2.32-2.24(m,1H),2.14-0.77(m,33H),1.34(s,3H),1.04(s,3H),0.96(s,6H),0.91(s,3H),0.87(s,3H),0.85(s,3H),0.84(s,3H),0.39-0.20(m,4H)；ESI-MS：m/z 889.65(M+H)⁺。

Step 2: synthesis of (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((S) -2- (1, 1-thiomorpholino) -3-methylbutyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-hydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid:

to (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((S) -2- (1, 1-thiomorphone dioxide)Quinolino) -3-methylbutyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-1H-cyclopenta [ a ] s]-9-yl) ester (step 1, 0.4g, 0.449mmol, 1.0eq) to a stirred solution in methanol (4ml) and THF (4ml) was added 2.5N aqueous KOH (1.35ml, 3.373mmol, 7.5 eq). The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was evaporated under reduced pressure, cooled to 0 ℃, adjusted to pH 5.0 with 1N HCl and extracted with DCM (2 × 25 ml). The combined organic layers were washed with water and Na₂SO₄Dried, filtered and concentrated under reduced pressure. The crude compound was purified by column chromatography on silica gel using 5% methanol in DCM as eluent to give the title compound (0.120g, 33.5% yield) as an off-white solid.¹H NMR(300MHz,DMSO-d6)：δppm 4.44(m,1H),3.06-2.95(m,8H),2.84-2.71(m,3H),2.45-2.24(m,5H),1.95-0.78(m,31H),1.26(s,3H),1.02(s,3H),0.95(s,3H),0.91(s,3H),0.89(s,3H),0.86(s,6H),0.83(s,3H),0.38-0.18(m,4H)；ESI-MS：m/z 799.60(M+H)⁺。

Example 7: preparation of (1R,3S) -3- (((((1R, 3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) - 3a- (((2R,3R) -2- (1, 1-thiomorpholino) -3-hydroxybutyl) amino) -5a,5b,8,8,11 a-pentamethyl- 1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a] -9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-one Formic acid:

step 1: synthesis of (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3R) -3- ((tert-butyldimethylsilyl) oxy) -2- (1,1-thiomorpholino) butyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) ester:

to a stirred solution of 4- ((2R,3R) -3- ((tert-butyldimethylsilyl) oxy) -1-hydroxybut-2-yl) thiomorpholine 1, 1-dioxide (intermediate 8, 0.442g, 1.311mmol, 1.5eq) in DCM (10ml) was added triflic anhydride (0.234ml, 1.399mmol, 1.6eq) at 0 ℃. The reaction mixture was stirred at 0 ℃ for 10 minutes. 2, 6-lutidine (0.166ml, 1.399mmol, 1.6eq) was added and stirred at 0 ℃ for about 10 minutes. (1S,3R) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3 a-amino-5 a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a-at 0 ℃ in succession]-9-yl). 3-benzyl ester (intermediate 1, 0.6g, 0.8745mmol, 1.0eq) and triethylamine (0.36ml, 2.623mmol, 3.0eq) and stirred at the same temperature for about 1 hour. The reaction mixture was brought to room temperature and stirred for about 4 hours. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was quenched with saturated sodium bicarbonate solution and extracted with DCM (2 × 30 ml). The combined organic layers were washed with water and Na₂SO₄Dried, filtered and concentrated under reduced pressure. The crude compound was purified by column chromatography on silica gel using 20% ethyl acetate/hexanes as eluent to give the title compound (0.4g, 45.5% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 7.36-7.33(m,5H),5.15,5.09(ABq,J_AB＝12.3Hz,2H),4.44(dd,J＝11.1,4.5Hz,1H),4.0-3.97(m,1H),3.46-3.35(m,2H),3.28-3.20(m,2H),2.98(m,4H),2.84-2.72(m,2H),2.70-2.60(m,1H),2.60-2.50(m,2H),2.42(m,2H),2.15-0.78(m,28H),1.34(s,3H),1.04(s,3H),0.96(s,6H),0.91(s,3H),0.87(brs,12H),0.85(s,3H),0.84(s,3H),0.38-0.20(m,4H),0.06(s,3H),0.05(s,3H)；ESI-MS：m/z 1005.85(M+H)⁺。

Step 2: synthesis of (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3R) -3- ((tert-butyldimethylsilyl) oxy) -2- (1, 1-thiomorpholino) butyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-hydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid:

to (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3R) -3- ((tert-butyldimethylsilyl) oxy) -2- (1, 1-thiomorpholino) butyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a]-9-yl) ester (step 1, 0.4g, 0.3977mmol, 1.0eq) to a stirred solution in methanol (5ml) and THF (5ml) was added 2.5N aqueous KOH (1.19ml, 2.983mmol, 7.5 eq). The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was evaporated under reduced pressure, cooled to 0 ℃, adjusted to pH 5.0 with 1N HCl, and extracted with DCM (2 × 20 ml). The combined organic layers were washed with water and Na₂SO₄Dried, filtered and concentrated under reduced pressure. The crude compound was purified by column chromatography on silica gel using 3% methanol in DCM as eluent to give the title compound (0.3g, 82.41% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 4.56-4.50(m,1H),3.99-3.96(m,1H),3.43-3.29(m,4H),3.17-3.03(m,2H),3.0-2.82(m,3H),2.78-2.63(m,2H),2.60-2.47(m,3H),2.02-0.78(m,29H),1.33(s,3H),1.03(s,3H),0.99(s,3H),0.94(s,3H),0.92(s,3H),0.89(s,3H),0.88(s,3H),0.87(s,9H),0.85(s,3H),0.40-0.22(m,4H),0.06(s,3H),0.04(s,3H)；ES-MS：m/z 915.60(M+H)⁺。

And step 3: synthesis of (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3R) -2- (1, 1-thiomorpholino) -3-hydroxybutyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid:

to (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3R) -3- ((tert-butyldimethylsilyl) oxy) -2- (1, 1-thiomorpholino) butyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a at 0 deg.C]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid (step 2, 0.3g, 0.327mmol, 1.0eq) to a stirred solution in THF (6ml) was added tetra-n-butylammonium fluoride (10ml, 10.0mmol, 31.0eq, 1.0M in THF). The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was diluted with water (20ml) and extracted with DCM (2 × 20 ml). The combined organic layers were washed with water and Na₂SO₄Dried, filtered and evaporated under reduced pressure. The crude compound was purified by column chromatography on silica gel using 5% methanol in DCM as eluent to give the title compound (65mg, 24.8% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm4.48-4.45(m,1H),3.75(m,1H),3.33(m,2H),3.20-3.05(m,6H),2.82-2.74(m,2H),2.63-2.53(m,5H),2.10-2.0(m,2H),1.97-0.78(m,26H),1.33(s,3H),1.07(s,6H),0.98(s,3H),0.91(s,3H),0.89(s,3H),0.86(s,6H),0.42-0.20(m,4H)；ES-MS：m/z 802.2(M+H)⁺。

Example 8: preparation of (1R,3S) -3- (((((1R, 3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) - 3a- (((S) -2- (1, 1-thiomorpholino) -3-hydroxypropyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1- Methylcyclopropyl) eicosahydro-1H-cyclopenta [ a] -9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid:

step 1: synthesis of (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((S) -3- ((tert-butyldimethylsilyl) oxy) -2- (1, 1-thiomorpholino) propyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) ester:

to a stirred solution of (S) -4- (1- ((tert-butyldimethylsilyl) oxy) -3-hydroxypropan-2-yl) thiomorpholine 1, 1-dioxide (intermediate 9, 0.844g, 2.623mmol, 1.5eq) in DCM (24ml) was added triflic anhydride (0.47ml, 2.798mmol, 1.6eq) at 0 ℃. The reaction mixture was stirred at 0 ℃ for about 10 minutes. 2, 6-lutidine (0.32ml, 2.798mmol, 1.6eq) was added and stirred at 0 ℃ for about 10 min. (1S,3R) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3 a-amino-5 a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a-at 0 ℃ in succession]-9-yl). 3-benzyl ester (intermediate 1, 1.2g, 1.749mmol, 1.0eq) and triethylamine (0.39ml, 2.798mmol, 1.6eq) and stirred at the same temperature for about 1 hour. The reaction mixture was brought to room temperature and stirred overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was diluted with DCM (150ml) and washed with saturated sodium bicarbonate solution, water and brine solution. Na for organic layer₂SO₄Dried, filtered and concentrated under reduced pressure. The crude compound was purified by column chromatography on silica gel using a 0-15% ethyl acetate/hexanes gradient to give the title compound (1g, 57.8% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 7.36-7.33(m,5H),5.15,5.09(ABq,J_AB＝12.3Hz,2H),4.44(dd,J＝11.1,4.8Hz,1H),3.71-3.67(m,2H),3.30-3.22(m,2H),3.18-3.06(m,2H),3.05-2.90(m,5H),2.83-2.72(m,3H),2.70-2.59(m,2H),2.46-2.32(m,2H),2.10-1.20(m,26H),1.0-0.78(m,31H),0.39-0.23(m,4H),0.05(s,6H)；ES-MS：m/z 992.09(M+H)⁺。

Step 2: synthesis of (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((S) -3- ((tert-butyldimethylsilyl) oxy) -2- (1, 1-thiomorpholino) propyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-hydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid:

to (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((S) -3- ((tert-butyldimethylsilyl) oxy) -2- (1, 1-thiomorpholino) propyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) ester (step 1, 1.0g, 1.008mmol, 1.0eq) to a stirred solution in methanol (12ml) and THF (12ml) was added 2.5N aqueous KOH (3.0ml, 7.564mmol, 7.5 eq). The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was evaporated under reduced pressure, cooled to 0 ℃, adjusted to pH 5.0 with 1N HCl, and extracted with DCM (2 × 100 ml). The combined organic layers were washed with water (100ml) and brine solution (100 ml). Na for organic layer₂SO₄Dried, filtered and concentrated under reduced pressure. The crude compound was purified by column chromatography on silica gel using 0-6% methanol in DCM as eluent to give the title compound (0.4g, 44.4% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 4.45(m,1H),3.69-3.65(m,2H),3.30(m,2H),3.10-2.96(m,7H),2.80-2.72(m,3H),2.60-2.50(m,2H),2.10-0.78(m,38H),1.36(s,3H),0.91(s,3H),0.88(s,9H),0.86(s,6H),0.35-0.27(m,4H),0.05(s,6H)；ES-MS：m/z 902.18(M+H)⁺。

And step 3: synthesis of (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((S) -2- (1, 1-thiomorpholino) -3-hydroxypropyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid:

to (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((S) -3- ((tert-butyldimethylsilyl) oxy) -2- (1, 1-thiomorpholino) propyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] at 0 deg.C]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid (step (a)Step 2, 0.4g, 0.444mmol, 1.0eq) to a stirred solution in THF (10ml) was added TBAF (0.532ml, 0.532mmol, 1.2eq, 1.0M in THF). The reaction mixture was stirred at room temperature for about 4 hours. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was evaporated under reduced pressure, diluted with water and extracted with DCM. Na for organic layer₂SO₄Dried, filtered and evaporated under reduced pressure. The residue was purified by column chromatography on silica gel using a gradient of 0-10% methanol in DCM to give the title compound (90mg, 25.7% yield) as an off-white solid.¹H NMR(300MHz,DMSO-d6)：δppm 4.40-4.32(m,1H),3.62-3.58(m,1H),3.50-3.45(m,1H),3.10-2.92(m,8H),2.82-2.70(m,3H),2.38-2.23(m,5H),1.92-0.78(m,24H),1.26(s,3H),0.99(s,3H),0.94(s,3H),0.91(s,3H),0.89(s,3H),0.83(s,6H),0.81(s,3H),0.33-0.22(m,4H)；ESI-MS：m/z 788.27(M+H)⁺。

Example 9: preparation of (1R,3S) -3- (((((1R, 3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) - 3a- (((S) -2- (1, 1-thiomorpholino) -3-fluoropropyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methyl) Cyclopropyl) eicosahydro-1H-cyclopenta [ a] -9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid:

step 1: synthesis of (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((S) -2- (1, 1-thiomorpholino) -3-fluoropropyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-hydro-1H-cyclopenta [ a ] a]9-yl) ester:

to a stirred solution of (S) -4- (1-fluoro-3-hydroxypropan-2-yl) thiomorpholine 1, 1-dioxide (intermediate 10, 0.922g, 4.372mmol, 3.0eq) in DCM (10ml) was added triflic anhydride (0.78ml, 4.664mmol, 3.2eq) at 0 ℃. The reaction mixture was stirred at 0 ℃ for about 10 minutes. 2, 6-lutidine (0.54ml, 4.664mmol, 3.2eq) was added and stirred at 0 ℃ for about 10 minutes. (1S,3R) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3 a-amino-5 a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a-at 0 ℃ in succession]-9-yl). 3-benzyl ester (intermediate 1, 1.0g, 1.457mmol, 1.0eq) and triethylamine (0.65ml, 4.664mmol, 3.2eq) and stirred at the same temperature for about 1 hour. The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was diluted with DCM (100ml) and washed with saturated sodium bicarbonate solution (100ml) and brine solution (100 ml). Na for organic layer₂SO₄Dried, filtered and concentrated under reduced pressure. The crude compound was purified by column chromatography on silica gel using a 0-20% ethyl acetate/hexanes gradient to give the title compound (0.300g, 23.4% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 7.36-7.33(m,5H),5.15,5.09(ABq,J_AB＝12.3Hz,2H),4.67(d,J＝4.5Hz,1H),4.51(d,J＝4.8Hz,1H),4.44(dd,J＝11.1,4.5Hz,1H),3.28-3.14(m,4H),3.11-3.07(m,1H),3.05-2.98(m,4H),2.85-2.73(m,3H),2.69-2.62(m,1H),2.53-2.47(m,3H),2.08-0.78(m,24H),1.34(s,3H),1.0(s,3H),0.96(s,3H),0.95(s,3H),0.91(s,3H),0.86(s,3H),0.85(s,3H),0.84(s,3H),0.40-0.20(m,4H)；ES-MS：m/z 880.01(M+H)⁺。

Step 2: synthesis of (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((S) -2- (1, 1-thiomorpholino) -3-fluoropropyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-hydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid:

to (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((S) -2- (1, 1-thiomorpholino) -3-fluoropropyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) ester (step 1, 0.300g, 0.341mmol, 1.0eq) to a stirred solution in methanol (3ml) and THF (3ml) was added 2.5N aqueous KOH (1ml, 2.559mmol, 7.5 eq). The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was evaporated under reduced pressure, cooled to 0 ℃, adjusted to pH 4.0 with 1N HCl, and extracted with DCM (2 × 100 ml). The combined organic layers were washed with water (100ml), dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude compound was purified by silica gel column chromatography using a 0-5% methanol/dichloromethane gradient. By using DCM: the resulting compound was further purified by treatment with hexane (1: 9 ratio), the solid was filtered and dried in vacuo to give the title compound (0.090g, 33.4% yield) as a white solid.¹H NMR(300MHz,CDCl₃)：δppm 4.67(d,J＝4.5Hz,1H),4.51(d,J＝4.5Hz,1H),4.46(dd,J＝11.1,4.2Hz,1H),3.23-3.0(m,9H),2.84-2.75(m,3H),2.64-2.56(m,4H),2.09-0.78(m,24H),1.37(s,3H),1.07(s,3H),1.01(s,3H),0.96(s,3H),0.91(s,3H),0.87(s,6H),0.85(s,3H),0.38-0.29(m,4H)；ES-MS：m/z 790.02(M+H)⁺。

Example 10: preparation of (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR, 13bR) -3a- (((R) -2- (1, 1-thiomorpholino) -3-fluoropropyl) amino) -5a,5b,8,8,11 a-pentamethyl- 1- (1-methylcyclopropyl) eicosahydro-1H-ringPentadieno [ a ]] -9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-one Formic acid:

step 1: synthesis of (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((R) -2- (1, 1-thiomorpholino) -3-fluoropropyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-hydro-1H-cyclopenta [ a ] a]9-yl) ester:

to a stirred solution of (R) -4- (1-fluoro-3-hydroxypropan-2-yl) thiomorpholine 1, 1-dioxide (intermediate 11, 0.645g, 3.061mmol, 3.0eq) in DCM (14ml) was added triflic anhydride (0.548ml, 3.265mmol, 3.2eq) at 0 ℃. After stirring at 0 ℃ for 10 minutes, 2, 6-lutidine (0.38ml, 3.265mmol, 3.2eq) was added and stirred at the same temperature for about 10 minutes. Reacting (1S,3R) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3 a-amino-5 a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl). 3-benzyl ester (intermediate 1, 0.700g, 1.020mmol, 1.0eq) and triethylamine (0.45ml, 3.265mmol, 3.2eq) were added to the reaction mixture and stirred at 0 ℃ for about 1 hour. The reaction mixture was stirred at room temperature overnight. TLC indicated that the starting material was complete and the desired product was observed. The reaction mixture was diluted with DCM (100ml) and saturated sodium bicarbonateThe solution (100ml), water and brine solution (100ml) were washed. The organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude compound was purified by column chromatography on silica gel using a 0-20% ethyl acetate/hexanes gradient to give the title compound (0.200g, 22.2% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 7.36-7.35(m,5H),5.15,5.09(ABq,J_AB＝12.3Hz,2H),4.64-4.62(m,1H),4.48-4.36(m,2H),3.27-3.22(m,2H),3.18-3.02(m,7H),2.85-2.72(m,3H),2.59-2.50(m,1H),2.42-2.32(m,1H),2.10-0.78(m,26H),1.34(s,3H),1.04(s,3H),0.97(s,6H),0.91(s,3H),0.86(s,3H),0.85(s,3H),0.84(s,3H),0.40-0.20(m,4H)；ES-MS：m/z 880.01(M+H)⁺。

Step 2: synthesis of (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((R) -2- (1, 1-thiomorpholino) -3-fluoropropyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-hydro-1H-cyclopenta [ a ] a]9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid:

to (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((R) -2- (1, 1-thiomorpholino) -3-fluoropropyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) ester (step 1, 0.200g, 0.227mmol, 1.0eq) to a stirred solution in methanol (2ml) and THF (2ml) was added 2.5N aqueous KOH (0.68ml, 1.706mmol, 7.5 eq). The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was evaporated under reduced pressure, cooled to 0 ℃, adjusted to pH 4.0 with 1N HCl, and extracted with DCM (2 × 100 ml). The combined organic layers were washed with water (100ml),dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude compound was purified by silica gel column chromatography using a 0-5% methanol/dichloromethane gradient. By using DCM: the resulting compound was further purified by treatment with hexane (1: 9 ratio), and the precipitate formed was filtered and dried under vacuum to give the title compound (0.080g, 44.6% yield) as a white solid.¹H NMR(300MHz,CDCl₃)：δppm 4.64-4.40(m,3H),3.30-2.98(m,9H),2.80-2.50(m,5H),2.20-0.78(m,26H),1.35(s,3H),1.05(s,3H),1.01(s,3H),0.92(s,9H),0.88(s,3H),0.87(s,3H),0.40-0.20(m,4H)；ES-MS：m/z 790.02(M+H)⁺。

Example 11: preparation of (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR, 13bR) -3a- (((S) -2- (4- (isopropylsulfonyl) piperazin-1-yl) propyl) amino) -5a,5b,8,8,11 a-pentamethyl- 1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a] -9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-one Formic acid:

step 1: synthesis of (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((S) -2- (4- (isopropylsulfonyl) piperazin-1-yl) propyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-hydro-1H-cyclopenta [ a ] a]-9-yl) ester:

to (S) -2- (4- (isopropylsulfonyl) piperazin-1-yl) propan-1-ol (intermediate 12, 0.547g, 2.186 mm) at 0 deg.Col, 1.5eq) to a stirred solution in DCM (30ml) was added trifluoromethanesulfonic anhydride (0.41ml, 2.478mmol, 1.7 eq). After stirring at 0 ℃ for 10 min, 2, 6-lutidine (0.28ml, 2.478mmol, 1.7eq) was added and stirred at 0 ℃ for about 10 min. To the reaction mixture was added (1S,3R) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3 a-amino-5 a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a in DCM (10ml)]-9-yl). 3-benzyl ester (intermediate 1, 1.0g, 1.457mmol, 1.0eq), then triethylamine (0.6ml, 4.372mmol, 3.0eq) is added and stirred at 0 ℃ for about 1 hour. The reaction mixture was stirred at room temperature for about 4 hours. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was diluted with water (150ml) and extracted with DCM (3 × 200 ml). The combined organic layers were washed with water (100ml) and brine solution (100 ml). The organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude compound was purified by column chromatography on silica eluting with 2% methanol in dichloromethane. Fractions containing the expected product were combined and concentrated under reduced pressure to give the title compound (0.350g, 26.3% yield) as a yellow semi-solid.¹H NMR(300MHz,CDCl₃)：δppm 7.35(m,5H),5.15,5.09(ABq,J_AB＝12.3Hz,2H),4.44(dd,J＝11.1,4.8Hz,1H),3.48-3.31(m,4H),3.23-3.18(m,1H),2.92-2.43(m,10H),2.10-0.78(m,35H),1.34(s,3H),1.10(s,3H),1.0(s,3H),0.96(s,3H),0.93(s,3H),0.87(s,3H),0.85(s,3H),0.84(s,3H),0.40-0.20(m,4H)；ES-MS：m/z 919.03(M+H)⁺。

Step 2: synthesis of (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((S) -2- (4- (isopropylsulfonyl) piperazin-1-yl) propyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid:

to (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((S) -2- (4- (isopropylsulfonyl) piperazin-1-yl) propyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a at 0 deg.C]-9-yl) ester (step 1, 0.350g, 0.381mmol, 1.0eq) to a stirred solution in methanol (3.5ml) and THF (3.5ml) was added 2.5N aqueous KOH (1.14ml, 2.858mmol, 7.5 eq). The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was evaporated under reduced pressure, cooled to 0 ℃, adjusted to pH 4.0 with 1N HCl and extracted with DCM (3 × 150 ml). The combined organic layers were washed with water (150ml), dried over sodium sulfate, filtered and evaporated under reduced pressure. The crude compound was purified by column chromatography on silica eluting with 6% methanol in dichloromethane. The fractions containing the expected product were combined and concentrated under reduced pressure. The resulting compound was dissolved in DCM (2ml), treated with hexane (10ml) and stirred at rt for about 15 min. The precipitate formed was collected by filtration and dried in vacuo to give the title compound (70mg, 22.2% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 4.47(m,1H),3.50-3.30(m,4H),3.25-3.15(m,1H),2.93(m,1H),2.83-2.73(m,2H),2.68-2.50(m,7H),2.10-0.78(m,35H),1.34(s,3H),1.17(s,3H),1.07(s,3H),1.03(s,3H),0.93(s,3H),0.90(s,3H),0.86(s,6H),0.52-0.22(m,4H)；ES-MS：m/z 829.01(M+H)⁺。

Example 12: preparation of (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR, 13bR) -3a- (((2R,3S) -2- (1, 1-thiomorpholino) -3-fluorobutyl) amino) -5a,5b,8,8,11 a-pentamethylene 1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] yl] -9-yl)Oxy) carbonyl) -2, 2-dimethylcyclobutane Alkane-1-carboxylic acid:

step 1: synthesis of (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3S) -2- (1, 1-thiomorpholino) -3-fluorobutyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) ester:

to a stirred solution of 4- ((2R,3S) -3-fluoro-1-hydroxybut-2-yl) thiomorpholine 1, 1-dioxide (intermediate 13, 0.590g, 2.623mmol, 2.0eq) in DCM (20ml) was added 2, 6-lutidine (0.45ml, 3.933mmol, 3.0eq) at 0 ℃. The reaction mixture was stirred at 0 ℃ for about 10 minutes, then trifluoromethanesulfonic anhydride (0.567ml, 3.277mmol, 2.5eq) was added and stirred at the same temperature for about 15 minutes. Reacting (1S,3R) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3 a-amino-5 a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]3-benzyl-9-yl (intermediate 1, 0.9g, 1.311mmol, 1.0eq) and triethylamine (0.55ml, 3.933mmol, 3.0eq) were added to the reaction mixture and stirred at 0 ℃ for about 4 hours. The reaction mixture was stirred at room temperature overnight. Saturated sodium bicarbonate solution was added to the reaction mixture and extracted with DCM (2 × 30 ml). The combined organic layers were washed with water, dried over sodium sulfate and concentrated under reduced pressure. The crude compound was purified by column chromatography on silica gel using 20% ethyl acetate/hexane as eluent to give the title compound(0.3g, 25.64% yield) as an off-white solid.¹HNMR(300MHz,CDCl₃)：δppm 7.36-7.34(m,5H),5.15,5.09(ABq,J_AB＝12.3Hz,2H),4.44(dd,J＝11.1,4.5Hz,1H),4.50-4.48(m,0.5H),4.33-4.31(m,0.5H),3.22-3.13(m,2H),3.10-3.0(m,7H),2.85-2.58(m,4H),2.55-2.43(m,1H),2.08-0.78(m,29H),1.34(s,3H),0.99(s,3H),0.96(s,6H),0.90(s,3H),0.85(s,6H),0.84(s,3H),0.40-0.20(m,4H)；ES-MS：m/z 893.91(M+H)⁺。

Step 2: synthesis of (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3S) -2- (1, 1-thiomorpholino) -3-fluorobutyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-hydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid:

to (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3S) -2- (1, 1-thiomorpholino) -3-fluorobutyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) ester (step 1, 0.3g, 0.335mmol, 1.0eq) to a stirred solution in methanol (3ml) and THF (3ml) was added 2.5N aqueous KOH (1.0ml, 2.518mmol, 7.5 eq). The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was evaporated, cooled to 0 ℃, adjusted to pH 5.0 with 1N HCl and extracted with DCM (2 × 25 ml). The combined organic layers were washed with water, dried over sodium sulfate and concentrated under reduced pressure. The crude compound was purified by column chromatography on silica gel using 2% methanol in dichloromethane as eluent to give the title compound (0.13g, 48.32% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 4.49-4.32(m,2H),3.21-3.04(m,9H),2.85-2.70(m,3H),2.64-2.48(m,2H),2.10-0.78(m,29H),1.33(s,3H),1.0(s,3H),0.96(s,3H),0.91(s,3H),0.86(s,9H),0.85(s,3H),0.40-0.22(m,4H)；ES-MS：m/z 803.82(M+H)⁺。

Example 13: preparation of (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR, 13bR) -3a- (((2S,3S) -2- (1, 1-thiomorpholino) -3-hydroxybutyl) amino) -5a,5b,8,8,11 a-penta Methyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a] -9-yl) oxy) carbonyl) -2, 2-dimethyl ring Butane-1-carboxylic acid:

step 1: synthesis of (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2S,3S) -3- ((tert-butyldimethylsilyl) oxy) -2- (1, 1-thiomorpholino) butyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-hydro-1H-cyclopenta [ a]-9-yl) ester:

to a stirred solution of 4- ((2S,3S) -3- ((tert-butyldimethylsilyl) oxy) -1-hydroxybut-2-yl) thiomorpholine 1, 1-dioxide (intermediate 14, 0.787g, 2.33mmol, 2.0eq) in DCM (16ml) was added triflic anhydride (0.31ml, 1.86mmol, 1.6eq) at 0 ℃. The reaction mixture was stirred at 0 ℃ for about 10 minutes. Subsequently, 2, 6-lutidine (0.21ml, 1.86mmol, 1) was added to the reaction mixture.6eq), stirred for about 10 minutes. (1S,3R) -2, 2-Dimethylcyclobutane-1, 3-dicarboxylic acid 1- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3 a-amino-5 a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a [ a ] is added to the reaction mixture]-9-yl). 3-benzyl ester (intermediate 1, 0.800g, 1.166mmol, 1.0eq) and then triethylamine (0.48ml, 3.498mmol, 3.0eq) is added and stirred at 0 ℃ for about 1 hour. The reaction mixture was brought to room temperature and stirred overnight. TLC indicated that the starting material was complete and the desired product was observed. The reaction mixture was quenched with aqueous sodium bicarbonate and extracted with DCM (3 × 100 ml). The combined organic layers were washed with water (100ml), dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude compound was purified by column chromatography on silica gel using a gradient of 20-30% ethyl acetate in hexanes to give the title compound (0.400g, 34% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 7.36-7.30(m,5H),5.15,5.09(ABq,J_AB＝12.3Hz,2H),4.45(dd,J＝11.1,4.5Hz,1H),3.95-3.92(m,1H),3.44-3.39(m,2H),3.21-3.11(m,2H),3.03-2.93(m,5H),2.84-2.73(m,2H),2.69-2.62(m,1H),2.59-2.53(m,2H),2.47-2.35(m,2H),2.08-0.78(m,30H),1.34(s,3H),1.0(s,3H),0.96(s,3H),0.96(s,3H),0.91(s,3H),0.87(s,9H),0.85(s,3H),0.84(s,3H),0.36-0.23(m,4H),0.05(s,3H),0.04(s,3H)；ES-MS：m/z 1005.97(M+H)⁺。

Step 2: synthesis of (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2S,3S) -3- ((tert-butyldimethylsilyl) oxy) -2- (1, 1-thiomorpholino) butyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-hydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid:

to (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2S,3S) -3- ((tert-butyldimethylsilyl) oxy) -2- (1, 1-thiomorpholino) butyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a]-9-yl) ester (step 1, 0.400g, 0.397mmol, 1.0eq) to a stirred solution in methanol (4ml) and THF (4ml) was added 1.5N aqueous KOH (1.9ml, 2.98mmol, 7.5 eq). The reaction mixture was stirred at room temperature for about 5 hours. TLC indicated that the starting material was complete and the desired product was observed. The reaction mixture was evaporated under reduced pressure, cooled to 0 ℃, acidified to pH 4.0 with 1N HCl and extracted with DCM (3 × 50 ml). The combined organic layers were washed with water (50ml), dried over sodium sulphate, filtered and evaporated under reduced pressure. The residue was purified by column chromatography on silica gel using a 0-6% methanol/dichloromethane gradient to give the title compound (0.200g, 55% yield) as a solid.¹H NMR(300MHz,CDCl₃)：δppm 4.49-4.44(m,1H),4.0-3.92(m,1H),3.40-3.22(m,4H),3.04-2.90(m,5H),2.80-2.70(m,3H),2.60-2.50(m,3H),2.32(m,1H),2.10-0.78(m,36H),1.33(s,3H),1.06(s,3H),1.04(s,3H),0.97(s,3H),0.95(s,3H),0.91(s,3H),0.89(s,6H),0.40-0.20(m,4H),0.04(s,3H),0.03(s,3H)；ES-MS：m/z 915.74(M+H)⁺。

And step 3: synthesis of (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2S,3S) -2- (1, 1-thiomorpholino) -3-hydroxybutyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-hydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid:

to (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2S,3S) -3- ((tert-butyldimethylsilyl) oxy) -2- (1, 1-thiomorpholino) butyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-hydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid (step 2, 0.200g, 0.218mmol, 1.0eq) to a stirred solution in THF (2ml) was added TBAF (0.654ml, 0.654mmol, 3.0eq, 1.0M in THF). The reaction mixture was stirred at room temperature overnight. TLC showed the starting material was not complete, TBAF (1.526ml, 1.526mmol, 7.0eq) was added again at 0 deg.C, brought to room temperature and stirred at room temperature overnight. TLC indicated that the starting material was complete and the desired product was observed. The reaction mixture was diluted with water (50ml) and extracted with DCM (3 × 30 ml). The combined organic layers were washed with water (50ml), dried over sodium sulphate, filtered and evaporated under reduced pressure. The residue was purified by column chromatography on silica gel using a 0-5% methanol/dichloromethane gradient. The resulting compound was further purified by treatment with ethyl acetate (1ml) and hexane (10ml), stirred at room temperature for about 10 minutes, filtered and dried under vacuum to give the title compound (0.080g, 45.7% yield) as a solid.¹H NMR(300MHz,CDCl₃)：δppm 4.46(dd,J＝11.7,4.8 Hz,1H),3.80(m,1H),3.42-3.32(m,2H),3.18-3.0(m,7H),2.80-2.50(m,7H),2.08-0.78(m,27H),1.36(s,3H),1.06(s,3H),1.03(s,3H),0.98(s,3H),0.91(s,3H),0.88(s,3H),0.85(s,6H),0.35-0.20(m,4H)；ES-MS：m/z 801.73(M+H)⁺。

Example 14: preparation of (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR, 13bR) -3a- (((2R,3R) -3-hydroxy-2- (4- (isopropylsulfonyl) piperazin-1-yl) butyl) amino) -5a,5b,8, 11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a] -9-yl) oxy) carbonyl) -2, 2-dimethyl Cyclobutane-1-carboxylic acid:

step 1: synthesis of (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3R) -3- ((tert-butyldimethylsilyl) oxy) -2- (4- (isopropylsulfonyl) piperazin-1-yl) butyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-hydro-1H-cyclopenta [ a]-9-yl) ester:

to a stirred solution of (2R,3R) -3- ((tert-butyldimethylsilyl) oxy) -2- (4- (isopropylsulfonyl) piperazin-1-yl) butan-1-ol (intermediate 15, 3.21g, 8.16mmol, 1.6eq) in DCM (50ml) was added trifluoromethanesulfonic anhydride (1.45ml, 8.67mmol, 1.7eq) at 0 ℃. The reaction mixture was stirred at 0 ℃ for about 10 minutes. 2, 6-lutidine (0.65ml, 8.67mmol, 1.7eq) was added and stirred at 0 ℃ for 15 min. Reacting (1S,3R) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3 a-amino-5 a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl). 3-benzyl ester (intermediate 1, 3.5g, 5.10mmol, 1.0eq) and triethylamine (2.13ml, 15.3mmol, 3.0eq) were added to the reaction mixture and stirred at 0 ℃ for about 1 hour. The reaction mixture was brought to room temperature and stirred overnight. TLC indicated that the starting material was complete and the desired product was observed. The reaction mixture was diluted with water (100ml), basified with aqueous sodium bicarbonate and extracted with DCM (3 × 100 ml). The combined organic layers were washed with water (100ml), dried over sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified by column chromatography on silica gel using a 0-2% methanol/dichloromethane gradient to give the title compound (2.0g, 36.9%Yield) as a solid.¹H NMR(300MHz,CDCl₃)：δppm 7.35(m,5H),5.15,5.09(ABq,J_AB＝12.6Hz,2H),4.44(dd,J＝11.1,4.5Hz,1H),4.01-3.98(m,1H),3.30-3.15(m,5H),2.90-2.32(m,10H),2.08-1.48(m,13H),1.47-1.13(m,24H),1.07-0.78(m,31H),0.38-0.18(m,4H),0.04(s,3H),0.03(s,3H)；ES-MS：m/z 1063.68(M+H)⁺。

Step 2: synthesis of (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3R) -3-hydroxy-2- (4- (isopropylsulfonyl) piperazin-1-yl) butyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-hydro-1H-cyclopenta [ a ] a]-9-yl) ester:

to (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3R) -3- ((tert-butyldimethylsilyl) oxy) -2- (4- (isopropylsulfonyl) piperazin-1-yl) butyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-hydro-1H-cyclopenta [ a ] a at 0 deg.C]To a stirred solution of (9-yl) ester (step 1, 2.0g, 1.88mmol, 1.0eq) in THF (20ml) was added TBAF (7.52ml, 7.52mmol, 4.0eq, 1.0M in THF). The reaction mixture was stirred at room temperature overnight. TLC indicated that the starting material was complete and the desired product was observed. The reaction mixture was diluted with water (100ml) and extracted with DCM (3 × 100 ml). The combined organic layers were washed with water (100ml), dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude compound was purified by column chromatography on silica gel using a 0-4% methanol/dichloromethane gradient to give the title compound (0.500g, 28% yield) as a solid.¹H NMR(300MHz,CDCl₃)：δppm7.36-7.31(m,5H),5.15,5.09(ABq,J_AB＝12.3Hz,2H),4.44(dd,J＝10.8,4.5Hz,1H),3.67-3.56(m,1H),3.42-3.33(m,4H),3.25-3.13(m,1H),2.95-2.57(m,9H),2.43-1.90(m,6H),1.88-1.18(m,32H),1.03-0.78(m,22H),0.25-0.24(m,4H)；ES-MS：m/z 948.93(M+H)⁺。

And step 3: synthesis of (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3R) -3-hydroxy-2- (4- (isopropylsulfonyl) piperazin-1-yl) butyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-hydro-1H-cyclopenta [ a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid:

to (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3R) -3-hydroxy-2- (4- (isopropylsulfonyl) piperazin-1-yl) butyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] at 0 deg.C]-9-yl) ester (step 2, 0.500g, 0.527mmol, 1.0eq) to a stirred solution in THF (5ml) and methanol (5ml) was added 1.5N aqueous KOH (2.63ml, 3.954mmol, 7.5 eq). The reaction mixture was brought to room temperature and stirred overnight. TLC indicated that the starting material was complete and the desired product was observed. The reaction mixture was evaporated under reduced pressure. The residue was diluted with water (20ml), cooled to 0 ℃, acidified to pH 4.0 with 1N HCl and extracted with DCM (3 × 50 ml). The combined organic layers were washed with water (50ml), dried over sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified by silica gel column chromatography using a 0-10% methanol/dichloromethane gradient. The resulting compound was treated with DCM (5ml) and hexane (40ml), stirred at room temperature for about 15 minutes and the solvent decanted. DCM (5ml) and hexane (20ml) were added again, stirred at room temperature for about 15 minutes, the precipitate formed was filtered and dried under vacuum for 2 hours to give the title compound (0.180g, 4)0% yield) as a solid.¹H NMR(300MHz,CDCl₃)：δppm 4.46(dd,J＝11.7,4.8Hz,1H),3.75(m,1H),3.35(m,4H),3.23-3.14(m,1H),2.92-2.88(m,2H),2.79-2.74(m,4H),2.61-2.51(m,4H),2.10-1.2(m,35H),1.10-0.78(m,24H),0.34-0.25(m,4H)；ES-MS：m/z 858.82(M+H)⁺。

Example 15: preparation of (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR, 13bR) -3a- (((2R,3R) -2- (1, 1-thiomorpholino) -3-hydroxybutyl) amino) -5a,5b,8,8,11 a-penta Methyl-1- (prop-1-en-2-yl) eicosa-1H-cyclopenta [ a] -9-yl) oxy) carbonyl) -2, 2-dimethyl ring Butane-1-carboxylic acid:

step 1: synthesis of (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3R) -3- ((tert-butyldimethylsilyl) oxy) -2- (1, 1-thiomorpholino) butyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-hydro-1H-cyclopenta [ a]-9-yl) ester:

to a stirred solution of 4- ((2R,3R) -3- ((tert-butyldimethylsilyl) oxy) -1-hydroxybut-2-yl) thiomorpholine 1, 1-dioxide (intermediate 8, 4.14g, 12.27mmol, 1.5eq) in DCM (80ml) was added triflic anhydride (2.33ml, 13.9mmol, 1.7eq) and stirred for about 10 minutes at 0 ℃. To the reaction mixture was added 2, 6-lutidine (1.6ml, 13).9mmol, 1.7eq) and stirred at 0 ℃ for about 15 minutes. Reacting (1S,3R) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3 a-amino-5 a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosahydro-1H-cyclopenta [ a]-9-yl). 3-benzyl ester (intermediate 16, 5.5g, 8.18mmol, 1.0eq) and triethylamine (3.42ml, 24.54mmol, 3.0eq) were added to the reaction mixture, stirred at 0 ℃ for about 1 hour and at room temperature overnight. TLC indicated that the starting material was complete and the desired product was observed. Aqueous sodium bicarbonate was added to the reaction mixture and extracted with DCM (3 × 100 ml). The combined organic layers were washed with water (100ml), dried over sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified by column chromatography on silica gel using a 0-5% methanol/dichloromethane gradient to give the title compound (4.0g, 49.3% yield) as an off-white solid.¹HNMR(300MHz,CDCl₃)：δppm 7.36-7.30(m,5H),5.14,5.09(ABq,J_AB＝12.3Hz,2H),4.69(s,1H),4.58(s,1H),4.43(dd,J＝11.7,4.5Hz,1H),4.03-3.98(m,1H),3.47-3.37(m,2H),3.30-3.18(m,2H),3.10-2.94(m,5H),2.85-2.40(m,7H),2.07-1.98(m,2H),1.93-0.75(m,25H),1.68(s,3H),1.33(s,3H),1.05(s,3H),0.96(s,3H),0.95(s,3H),0.88(s,9H),0.85(s,3H),0.84(s,3H),0.83(s,3H),0.07(s,6H)；ES-MS：m/z 991.93(M+H)⁺。

Step 2: synthesis of (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3R) -2- (1, 1-thiomorpholino) -3-hydroxybutyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-hydro-1H-cyclopenta [ a]-9-yl) ester:

to (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl at 0 ℃3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3R) -3- ((tert-butyldimethylsilyl) oxy) -2- (1, 1-thiomorpholino) butyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosahydro-1H-cyclopenta [ a]To a stirred solution of (9-yl) ester (step 1, 4.0g, 4.034mmol, 1.0eq) in THF (60ml) was added TBAF (20.17ml, 20.17mmol, 5.0eq, 1.0M in THF). The reaction mixture was brought to room temperature and stirred overnight. TLC indicated that the starting material was complete and the desired product was observed. The reaction mixture was diluted with water (50ml) and extracted with DCM (3 × 50 ml). The combined organic layers were washed with water (2 × 50ml), dried over sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified by column chromatography on silica gel using a gradient of 0-5% methanol in DCM to give the title compound (2.0g, 56.6% yield) as a solid.¹H NMR(300MHz,CDCl₃)：δppm 7.35(m,5H),5.14,5.09(ABq,J_AB＝12.3Hz,2H),4.69(s,1H),4.60(s,1H),4.45-4.40(m,1H),3.72(m,1H),3.45-3.35(m,2H),3.13-3.03(m,7H),2.85-2.73(m,2H),2.70-2.58(m,2H),2.57-2.42(m,3H),2.07-1.98(m,2H),1.90-0.75(m,25H),1.68(s,3H),1.34(s,3H),1.05(s,3H),0.96(s,6H),0.85(s,3H),0.84(s,3H),0.83(s,3H)；ES-MS：m/z 877.84(M+H)⁺。

And step 3: synthesis of (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3R) -2- (1, 1-thiomorpholino) -3-hydroxybutyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-hydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid:

to (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3R) -2- (1, 1-bis-methyl-cyclobutane) at 0 deg.CThiomorpholino) -3-hydroxybutyl oxide) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-1H-cyclopenta [ a ] s]-9-yl) ester (step 2, 2.0g, 2.279mmol, 1.0eq) to a stirred solution in THF (12ml) and methanol (12ml) was added 1.5N aqueous KOH (12ml, 17.09mmol, 7.5 eq). The reaction mixture was brought to room temperature and stirred for about 4 hours. TLC indicated that the starting material was complete and the desired product was observed. The reaction mixture was evaporated under reduced pressure, diluted with water (20ml), acidified to pH 4.0 with 1N HCl and extracted with DCM (3 × 50 ml). The combined organic layers were washed with water (20ml), dried over sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified by silica gel column chromatography using a gradient of 4-8% methanol in DCM. The resulting compound was further purified by treatment with chloroform (5ml) and hexane (20ml), stirred for about 10 minutes and decanted. This procedure was repeated twice, and finally filtered and dried in vacuo to give the title compound (0.800g, 44.6% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 4.69(s,1H),4.60(s,1H),4.50-4.45(m,1H),3.83-3.74(m,1H),3.40-3.07(m,9H),2.80-2.49(m,7H),2.07-0.77(m,27H),1.68(s,3H),1.36(s,3H),1.10(s,3H),1.06(s,3H),0.97(s,3H),0.89(s,3H),0.85(s,6H)；ES-MS：m/z 787.60(M+H)⁺。

Example 16: preparation of (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR, 13bR) -3a- (((R) -2-carboxy-2- (1, 1-thiomorpholino) ethyl) amino) -5a,5b,8,8,11 a-pentamethylene 1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] yl] -9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane Alkane-1-carboxylic acid:

step 1: synthesis of (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((R) -2- (1, 1-thiomorpholino) -3-methoxy-3-oxopropyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]9-yl) ester:

to a stirred solution of (R) -methyl 2- (1, 1-thiomorpholino) -3-hydroxypropionate (intermediate 5, 0.34g, 1.457mmol, 1.25eq) in dichloromethane (16ml) was added trifluoromethanesulfonic anhydride (0.27ml, 1.632mmol, 1.4eq) at 0 ℃. The reaction mixture was stirred at 0 ℃ for 10 minutes, then 2, 6-lutidine (0.19ml, 1.667mmol, 1.43eq) was added and stirred at the same temperature for 10 minutes. Reacting (1S,3R) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3 a-amino-5 a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl). 3-benzyl ester (intermediate 1, 0.8g, 1.166mmol, 1.0eq) followed by triethylamine (0.23ml, 1.667mmol, 1.43eq) was added to the reaction mixture and stirred at 0 ℃ for about 1 hour. The reaction mixture was brought to room temperature and stirred for about 4 hours. TLC indicated that the starting material was complete and the desired product was observed. The reaction mixture was basified with saturated sodium bicarbonate solution and extracted with dichloromethane (3 × 50 ml). The combined organic layers were washed with water (20ml) and Na₂SO₄Dried, filtered and concentrated under reduced pressure. The crude compound was purified by column chromatography on silica gel using a 0-3% methanol/dichloromethane gradient to give the title compound (0.680g, 64.7% yield) as a solid.¹H NMR(300MHz,CDCl₃)：δppm 7.35(m,5H),5.15,5.09(ABq,J_AB＝12.3Hz,2H),4.44(dd,J＝11.1,4.5Hz 1H),3.70(s,3H),3.42-3.35(m,1H),3.35-3.25(m,1H),3.15-2.94(m,7H),2.85-2.57(m,5H),2.08-1.0(m,28H),1.0-0.78(m,22H),0.38-0.20(m,4H)。

Step 2: synthesis of (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((R) -2- (1, 1-thiomorpholino) -3-methoxy-3-oxopropyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid:

to a suspension of 10% Pd/C (500mg) in ethyl acetate (7ml) and methanol (7ml) was added (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((R) -2- (1, 1-thiomorpholino) -3-methoxy-3-oxopropyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) ester (step 1, 0.35g, 0.386mmol, 1.0eq) and ammonium formate (0.121g, 1.933mmol, 5.0 eq). The reaction mixture was stirred at room temperature for about 1 hour. TLC indicated that the starting material was complete and the desired product was observed. The reaction mixture was filtered through a pad of celite and washed with 5% methanol/DCM (100 ml). The filtrate was evaporated under reduced pressure and the residue was purified by silica gel column chromatography using a gradient of 5 to 10% methanol/DCM and one drop of acetic acid. The resulting solid was treated with ethyl acetate (2mL) and hexane (8mL) and stirred at room temperature for 20 minutes. The precipitate formed was collected by filtration and dried in vacuo to give the title compound (40mg, 12.69% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 4.47-4.45(m,1H),3.70(s,3H),3.42-3.35(m,1H),3.28(m,1H),3.15-3.02(m,4H),2.98(m,3H),2.85-2.75(m,2H),2.65-2.52(m,3H),2.10-1.18(m,25H),1.10-0.78(m,25H),0.40-0.20(m,4H)；ES-MS：m/z 815.88(M+H)⁺。

Step-3: synthesis of (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((R) -2-carboxy-2- (1, 1-thiomorpholino) ethyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-hydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid:

to (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((R) -2- (1, 1-thiomorpholino) -3-methoxy-3-oxopropyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]To a stirred solution of (0.3g, 0.33mmol, 1.0eq) of the (9-yl) ester in methanol (3ml) and THF (3ml) was added 2.5N aqueous KOH (1.0ml, 2.48mmol, 7.5 eq). The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was evaporated under reduced pressure, diluted with water (10ml), cooled to 0 ℃, acidified to pH 2.0 with 1N HCl and extracted with DCM (3 × 25 ml). The combined organic layers were washed with water (20ml) and Na₂SO₄Dried, filtered and concentrated under reduced pressure. The residue was purified by column chromatography on silica using one drop of acetic acid/10% methanol/DCM as eluent. The resulting compound was further purified by treatment with ethyl acetate (20ml), which was heated under reflux for about 30 minutes. The reaction mixture was cooled to room temperature, the solid was filtered, washed with ethyl acetate (5ml) and dried in vacuo to give the title compound (50mg, 18.8% yield) as a white solid.¹H NMR(300MHz,DMSO-d₆)：δppm 4.38-4.32(m,1H),3.08-2.93(m,8H),2.82-2.68(m,4H),2.37-2.23(m,2H),2.0-0.78(m,26H),1.27(s,3H),1.05(s,3H),0.95(s,3H),0.91(s,6H),0.85(s,3H),0.84(s,3H),0.83(s,3H),0.4-0.18(m,4H)；ESI-MS：m/z 823.55(M+Na)⁺。

Example 17: preparation of (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR, 13bR) -3a- (((2R,3R) -2- (1, 1-thiomorpholino) -3- (2- (2-methoxyethoxy) acetoxy) butan Yl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] s] -9-yl) Oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid:

to (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3R) -2- (1, 1-thiomorpholino) -3-hydroxybutyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid (example 8,0.400g, 0.499mmol, 1.0eq) to a stirred solution in DCM (10ml) was added DMAP (0.030g, 0.24mmol, 0.5eq), edc.hcl (0.430g, 2.24mmol, 4.5eq) and 2- (2-methoxyethoxy) acetic acid (intermediate 17, 0.167g, 1.24mmol, 2.5eq, step-2). The reaction mixture was stirred at room temperature overnight. TLC indicated that the starting material was complete and the desired product was observed. The reaction mixture was diluted with water (10ml) and extracted with DCM (3 × 20 ml). The combined organic layers were washed with water (20ml), dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude compound was purified by silica gel column chromatography using a 0-4% methanol/dichloromethane gradient. The resulting compound was further purified by treatment with chloroform (2ml) and hexane (8ml), stirred at room temperature for about 30 minutes, filtered and dried under vacuum to obtain the title compound (0.100g, 21.8% yield) as a solid.¹H NMR(300MHz,CDCl₃)：δppm 5.15-5.09(m,1H),4.48(m,1H),4.16(s,2H),3.73-3.71(m,2H),3.62-3.58(m,2H),3.38(s,3H),3.27-3.19(m,4H),3.0-2.95(m,5H),2.85-2.73(m,2H),2.65-2.55(m,1H),2.50-2.35(m,2H),2.08-2.0(m,2H),1.90-0.78(m,27H),1.36(s,3H),1.09(s,3H),1.06(s,3H),0.98(s,3H),0.90(s,3H),0.90(s,3H),0.87(s,3H),0.87(s,3H),0.38-0.25(m,4H)；ES-MS：m/z 917.95(M+H)⁺。

Example 18: preparation of (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR, 13bR) -3a- (((12R,13R) -13- (1, 1-thiomorpholino) -12-methyl-2, 5,8, 11-tetraoxatetradecane- 14-yl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a] - 9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid:

step 1: synthesis of (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((12R,13R) -13- (1, 1-thiomorpholino) -12-methyl-2, 5,8, 11-tetraoxatetradecan-14-yl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosa-hydro-1H-cyclopenta [ a]-9-yl) ester:

to a stirred solution of 4- ((12R,13R) -14-hydroxy-12-methyl-2, 5,8, 11-tetraoxatetradec-13-yl) thiomorpholine 1, 1-dioxide (intermediate 18, 0.775g, 2.099mmol, 1.6eq) in DCM (20ml) was added trifluoromethanesulfonic acid at 0 deg.CAnhydride (0.39ml, 2.361mmol, 1.8 eq). After stirring at 0 ℃ for 10 minutes, 2, 6-lutidine (0.3ml, 2.623mmol, 2.0eq) was added and stirred at the same temperature for about 10 minutes. Reacting (1S,3R) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3 a-amino-5 a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]3-benzyl-9-yl (intermediate 1, 0.900g, 1.311mmol, 1.0eq) and triethylamine (0.5ml, 3.935mmol, 3.0eq) were added to the reaction mixture and stirred at 0 ℃ for about 1 hour. The reaction mixture was stirred at room temperature overnight. TLC indicated that the starting material was complete and the desired product was observed. The reaction mixture was diluted with DCM (100ml) and washed with saturated sodium bicarbonate solution (100ml), water and brine solution. The organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude product was purified by silica gel column chromatography using a 0-5% methanol/dichloromethane gradient to give the title compound (1.0g, 73.5% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 7.35(m,5H),5.15,5.09(ABq,J_AB＝12.6Hz,2H),4.44(dd,J＝10.5,4.2Hz,1H),3.67-3.65(m,4H),3.64-3.61(m,6H),3.60-3.58(m,1H),3.57-3.52(m,3H),3.45-3.40(m,1H),3.38(s,3H),3.36-3.27(m,1H),3.23-3.10(m,1H),3.09-3.06(m,1H),3.05-2.92(m,6H),2.83-2.64(m,3H),2.60-2.54(m,3H),2.10-0.78(m,27H),1.34(s,3H),1.03(s,3H),0.96(s,3H),0.95(s,3H),0.91(s,3H),0.89(s,3H),0.86(s,3H),0.85(s,3H),0.40-0.19(m,4H)；ES-MS：m/z 1038.95(M+H)⁺。

Step 2: synthesis of (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((12R,13R) -13- (1, 1-thiomorpholino) -12-methyl-2, 5,8, 11-tetraoxatetradecan-14-yl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a]9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid:

to (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((12R,13R) -13- (1, 1-thiomorpholino) -12-methyl-2, 5,8, 11-tetraoxatetradecan-14-yl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (1-methylcyclopropyl) eicosahydro-1H-cyclopenta [ a ] a at 0 deg.C]To a stirred solution of (9-yl) ester (step 1, 1.0g, 0.963mmol, 1.0eq) in THF (10ml) and methanol (10ml) was added 2.5N aqueous KOH (2.89ml, 7.229mmol, 7.5 eq). The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was evaporated under reduced pressure, cooled to 0 ℃, acidified to pH 4.0 with 1n hcl, and extracted with DCM (2 × 100 ml). The combined organic layers were washed with water (100ml) and Na₂SO₄Dried, filtered and concentrated under reduced pressure. The crude product was purified by silica gel column chromatography using a gradient of 0-7% methanol in DCM to give the title compound (0.050g, 5.47% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 4.46(dd,J＝11.4,4.8Hz,1H),3.65-3.50(m,14H),3.42-3.32(m,5H),3.18-3.10(m,2H),3.05-2.95(m,6H),2.82-2.72(m,3H),2.70-2.50(m,3H),2.08-0.76(m,27H),1.36(s,3H),1.07(s,3H),1.02(s,3H),0.96(s,3H),0.91(s,3H),0.88(s,3H),0.86(s,3H),0.86(s,3H),0.40-0.20(m,4H)；ES-MS：m/z 947.99(M+H)⁺。

Example 19: preparation of (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR, 13bR) -3a- (((2R,3R) -2- (1, 1-thiomorpholino) -3- (2- (2-methoxyethoxy) acetoxy) butan Yl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-1H-cyclopenta [ a ] s] -9-yl) Oxy) carbonyl) -2, 2-dimethylcyclobutane-1-Formic acid:

to (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3R) -2- (1, 1-thiomorpholino) -3-hydroxybutyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosahydro-1H-cyclopenta [ a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid (example-15, 0.650g, 0.825mmol, 1.0eq) to a stirred solution in DCM (13ml) was added 2- (2-methoxyethoxy) acetic acid (intermediate 17, 0.276g, 2.06mmol, 2.5eq), DMAP (0.050g, 0.41mmol, 0.5eq) and edc.hcl (0.711g, 3.71mmol, 4.5 eq). The reaction mixture was stirred at room temperature overnight. TLC indicated that the starting material was complete and the desired product was observed. The reaction mixture was diluted with water (50ml) and extracted with DCM (3 × 50 ml). The combined organic layers were washed with water (50ml), dried over sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified by column chromatography on silica gel using a 0-6% methanol/dichloromethane gradient. The resulting compound was further purified by treatment with ethyl acetate (5ml) and hexane (50ml), stirred at room temperature for about 30 minutes, filtered and dried under vacuum to give the title compound (0.480g, 64% yield) as a white solid.¹H NMR(300MHz,CDCl₃)：δppm 5.14-5.09(m,1H),4.68(s,1H),4.59(s,1H),4.53-4.48(m,1H),4.17(s,2H),3.75-3.68(m,2H),3.62-3.57(m,2H),3.38(s,3H),3.33-2.85(m,9H),2.81-2.40(m,5H),2.05-0.77(m,26H),1.68(s,3H),1.38(s,3H),1.24(d,J＝6.6Hz,3H),1.12(s,3H),1.06(s,3H),0.97(s,3H),0.90(s,3H),0.86(s,3H),0.85(s,3H)；ES-MS：m/z 904.04(M+H)⁺。

Example 20: preparation of 4- (((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- ((2R,3R) -2- (1, 1-thiomorpholino) -3-hydroxybutyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-1H-cyclopenta [ a)] -9-yl) oxy) -2, 2-dimethyl-4-oxobutanoic acid:

step 1: synthesis of acetic acid (1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- ((tert-butoxycarbonyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-hydro-1H-cyclopenta [ a]-9-yl ester:

to ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -9-hydroxy-5 a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-hydro-3 aH-cyclopenta [ a-a ] at 0 ℃]-3 a-yl) carbamic acid tert-butyl ester (prepared as described in WO 2017/149518A 2, 12g, 22.734mmol, 1.0eq) to a stirred solution in THF (120ml) was added triethylamine (9.5ml, 68.203mmol, 3.0eq), DMAP (0.333g, 2.728mmol, 0.12eq) and acetic anhydride (3ml, 31.828mmol, 1.4 eq). The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was diluted with ethyl acetate (200ml) and washed with water (2 × 200 ml). The organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude compound was treated with n-hexane (100ml), stirred at room temperature for about 1 hour, filtered and dried under vacuum to give the title compound (12g, 93% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 4.71(s,1H),4.60(s,1H),4.47(dd,J＝9.9,4.5Hz,1H),4.34(s,1H),2.58-2.30(m,3H),2.05(s,3H),2.02-0.77(m,22H),1.68(s,3H),1.44(s,9H),1.01(s,3H),0.95(s,3H),0.85(s,3H),0.84(s,3H),0.83(s,3H)；ES-MS：m/z 570.39(M+H)⁺。

Step 2: synthesis of acetic acid (1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3 a-amino-5 a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-hydro-1H-cyclopenta [ a]-9-yl ester:

to acetic acid (1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- ((tert-butoxycarbonyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosahydro-1H-cyclopenta [ a]To a stirred solution of the-9-yl ester (step 1,12 g, 21.057mmol, 1.0eq) in 1, 4-dioxane (24ml) was added 4N HCl in 1, 4-dioxane (24 ml). The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was concentrated under reduced pressure and basified with saturated sodium bicarbonate solution. The resulting solid was filtered and washed with water. The solid was dissolved in DCM, dried over sodium sulfate, filtered and concentrated under reduced pressure. The solid was further purified by trituration with n-hexane (100ml), filtered, washed with hexane and dried under vacuum to give the title compound (7g, 70.7% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 4.71(s,1H),4.59(s,1H),4.47(dd,J＝9.0,5.4Hz,1H),2.57-2.48(m,1H),2.04(s,3H),1.68(s,3H),1.66-0.77(m,24H),1.04(s,3H),0.95(s,3H),0.85(s,3H),0.84(s,3H),0.83(s,3H)；ES-MS：m/z 470.44(M+H)⁺。

And step 3: synthesis of acetic acid (1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3R) -3- ((tert-butyldimethylsilyl) oxy) -2- (1, 1-thiomorpholino) butyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-hydro-1H-cyclopenta [ a]-9-yl ester:

to a stirred solution of 4- ((2R,3R) -3- ((tert-butyldimethylsilyl) oxy) -1-hydroxybut-2-yl) thiomorpholine 1, 1-dioxide (intermediate-8, 8.5g, 25.326mmol, 1.7eq) in DCM (70ml) was added 2, 6-lutidine (3.8ml, 32.775mmol, 2.2eq) at 0 ℃. After stirring at 0 ℃ for 10 min, trifluoromethanesulfonic anhydride (5ml, 29.795mmol, 2.0eq) was added and stirred at 0 ℃ for about 20 min. Acetic acid (1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3 a-amino-5 a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-hydro-1H-cyclopenta [ a ] a]-9-yl ester (step 2, 7g, 14.897mmol, 1.0eq) and triethylamine (7.27ml, 52.142mmol, 3.5eq) were added to the reaction mixture and stirred at 0 ℃ for about 1 hour. The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was diluted with DCM (200ml) and washed with saturated sodium carbonate solution (100ml) and water (100 ml). The organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude product was purified by silica gel column chromatography using a 0-10% ethyl acetate/hexane gradient. The resulting compound was further purified by treatment with n-hexane, filtered and dried under vacuum to give the title compound (7.0g, 59% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 4.68(s,1H),4.58(s,1H),4.49-4.44(m,1H),4.08-4.0(m,1H),3.44-3.39(m,2H),3.28-3.22(m,2H),3.06-3.01(m,4H),2.60-2.40(m,4H),2.04(s,3H),1.93-0.76(m,27H),1.68(s,3H),1.04(s,3H),0.94(s,3H),0.87(s,9H),0.85(s,3H),0.84(s,3H),0.83(s,3H),0.07(s,6H)；ES-MS：m/z 789.67(M+H)⁺。

And 4, step 4: synthesis of 4- ((2R,3R) -3- ((tert-butyldimethylsilyl) oxy) -1- (((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -9-HydroxyiThe radical-5 a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-3 aH-cyclopenta [ a]-3 a-yl) amino) but-2-yl) thiomorpholine 1, 1-dioxide:

to acetic acid (1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3R) -3- ((tert-butyldimethylsilyl) oxy) -2- (1, 1-thiomorpholino) butyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosahydro-1H-cyclopenta [ a]To a stirred solution of the-9-yl ester (step 3, 7.0g, 8.868mmol, 1.0eq) in methanol (70ml) and THF (70ml) was added 1N aqueous KOH (53.2ml, 53.212mmol, 6.0 eq). The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was concentrated under reduced pressure, DCM (400ml) was added and washed with water (200 ml). The organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude product was purified by silica gel column chromatography using a gradient of 0-30% ethyl acetate in hexanes to give the title compound (6.0g, 91% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 4.69(s,1H),4.58(s,1H),4.04-3.99(m,1H),3.44-3.39(m,2H),3.28-3.16(m,3H),3.07-2.96(m,4H),2.61-2.40(m,4H),1.97-0.66(m,27H),1.68(s,3H),1.04(s,3H),0.96(s,3H),0.95(s,3H),0.87(s,9H),0.82(s,3H),0.75(s,3H),0.07(s,6H)；ES-MS：m/z 747.74(M+H)⁺。

And 5: synthesis of 2, 2-Dimethylsuccinate 1- (tert-butyl). 4- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3R) -3- ((tert-butyldimethylsilyl) oxy) -2- (1, 1-thiomorpholino) butyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosahydro-1H-cyclopenta [ a ] a]-9-yl) ester:

to 4- ((2R,3R) -3- ((tert-butyldimethylsilyl) oxy) -1- (((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -9-hydroxy-5 a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-hydro-3 aH-cyclopenta [ a]-3 a-yl) amino) but-2-yl) thiomorpholine 1, 1-dioxide (step 4, 1g, 1.338mmol, 1.0eq) to a stirred solution in DCM (20ml) was added edc.hcl (0.897g, 4.683mmol, 3.5eq), DMAP (0.081g, 0.669mmol, 0.5eq) and 4- (tert-butoxy) -3, 3-dimethyl-4-oxobutanoic acid (prepared as described in WO 2013/020245, 0.676g, 3.345mmol, 2.5 eq). The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was diluted with DCM (100ml) and washed with water (100 ml). The organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude product was purified by silica gel column chromatography using a gradient of 0-30% ethyl acetate in hexanes to give the title compound (1g, 80.2% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 4.68(s,1H),4.58(s,1H),4.48(dd,J＝10.5,6.0Hz,1H),4.02-3.99(m,1H),3.47-3.39(m,2H),3.27-3.22(m,2H),3.0(m,4H),2.62-2.40(m,4H),2.53(s,2H),1.97-0.75(m,33H),1.68(s,3H),1.43(s,9H),1.04(s,3H),0.94(s,3H),0.87(s,9H),0.84(s,6H),0.82(s,3H),0.07(s,6H)；ES-MS：m/z 932.06(M+H)⁺。

Step 6: synthesis of 2, 2-Dimethylsuccinate 1- (tert-butyl). 4- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3R) -2- (1, 1-thiomorpholino) -3-hydroxybutyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-hydro-1H-cyclopenta [ a]-9-yl) ester:

to 2, 2-dimethylsuccinate 1- (tert-butyl) · 4- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3R) -3- ((tert-butyldimethylsilyl) oxy) -2- (1, 1-thiomorpholino) butyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosahydro-1H-cyclopenta [ a ] a]To a stirred solution of (9-yl) ester (step 1, 1g, 1.073mmol, 1.0eq) in THF (10ml) was added TBAF (4.29ml, 4.294mmol, 4.0eq, 1.0M in THF). The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was diluted with ethyl acetate (100ml) and washed with water (100 ml). The organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude product was purified by silica gel column chromatography using a 0-4% methanol/dichloromethane gradient to give the title compound (0.800g, 91.2% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 4.68(s,1H),4.59(s,1H),4.48(dd,J＝10.2,6.0Hz,1H),3.73-3.68(m,1H),3.42-3.37(m,2H),3.18-3.05(m,6H),2.65-2.45(m,6H),1.96-0.76(m,24H),1.68(s,3H),1.43(s,9H),1.26(d,J＝6.0Hz,3H),1.23(s,3H),1.22(s,3H),1.04(s,3H),0.94(s,3H),0.84(s,6H),0.82(s,3H)；ES-MS：m/z 818.12(M+H)⁺。

And 7: synthesis of 4- (((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3R) -2- (1, 1-thiomorpholino) -3-hydroxybutyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-hydro-1H-cyclopenta [ a ] a]-9-yl) oxy) -2, 2-dimethyl-4-oxobutanoic acid:

to 2, 2-Dimethylsuccinic acid 1- (tert-butyl) · 4- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3R) -2- (1, 1-thiomorpholino) -3-hydroxybutyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-1H-cyclopenta [ a]-9-yl) ester (step 2, 0.800g, 0.978mmol, 1.0eq) to a stirred solution in 1, 4-dioxane (2ml) was added 4N HCl in 1, 4-dioxane (2 ml). The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was concentrated under reduced pressure. The crude compound was dissolved in DCM (100ml) and washed with water (100 ml). The organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude compound was purified by silica gel column chromatography using a 0-5% methanol/dichloromethane gradient. By using DCM: the resulting compound was further purified by recrystallization from hexane (1: 5, 10 ml). The mixture was cooled, filtered, washed with hexanes and dried in vacuo to give the title compound (0.500g, 67% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 4.69(s,1H),4.59(s,1H),4.49-4.44(m,1H),3.73(m,1H),3.42-3.37(m,2H),3.20-3.05(m,6H),2.70-2.48(m,6H),1.95-0.75(m,33H),1.67(s,3H),1.05(s,3H),0.96(s,3H),0.84(s,3H),0.83(s,3H),0.80(s,3H)；ES-MS：m/z 762.0(M+H)⁺。

Example 21: preparation of (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR, 13bR) -3a- (((15R,16R) -16- (1, 1-thiomorpholino) -15-methyl-13-oxo-2, 5,8,11, 14-penta Oxadedecan-17-yl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-hydro-1H-cyclopenta-diene Ene and [ a ]] -9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid:

to (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((2R,3R) -2- (1, 1-thiomorpholino) -3-hydroxybutyl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosahydro-1H-cyclopenta [ a]-9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid (example 15,0.350g, 0.444mmol, 1.0eq) to a stirred solution in DCM (5ml) were added EDC.HCl (0.383g, 2.000mmol, 4.5eq), DMAP (0.027g, 0.222mmol, 0.5eq) and 2,5,8, 11-tetraoxatridec-13-oic acid (0.247g, 1.111mmol, 2.5 eq). The reaction mixture was stirred at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was diluted with DCM (100ml) and washed with 1N HCl (50ml) and water (100 ml). The organic layer was dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude compound was purified by silica gel column chromatography using a 0-5% methanol/dichloromethane gradient. By using 1: the resulting compound was further purified by treatment with 8 (MTBE: hexane; 18ml), filtered, washed with n-hexane and dried under vacuum to give the title compound (60mg, 13.6% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 5.13-5.08(m,1H),4.68(s,1H),4.59(s,1H),4.53-4.48(m,1H),4.23-4.17(m,2H),3.78-3.63(m,10H),3.57-3.54(m,2H),3.38(s,3H),3.30-2.86(m,8H),2.82-2.73(m,2H),2.63-2.32(m,6H),2.10-0.78(m,27H),1.68(s,3H),1.38(s,3H),1.11(s,3H),1.06(s,3H),0.97(s,3H),0.89(s,3H),0.86(s,3H),0.85(s,3H)。

Example 22: preparation of (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR, 13bR) -3a- (((12R,13R) -13- (1, 1-thiomorpholino) -12-methyl-2, 5,8, 11-tetraoxatetradecane- 14-yl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-hydro-1H-cyclopenta [ a] -9- Yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid:

step 1: synthesis of acetic acid (1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((12R,13R) -13- (1, 1-thiomorpholino) -12-methyl-2, 5,8, 11-tetraoxatetradecan-14-yl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosan-1H-cyclopenta [ a ] a]-9-yl ester:

to a stirred solution of 4- ((12R,13R) -14-hydroxy-12-methyl-2, 5,8, 11-tetraoxatetradec-13-yl) thiomorpholine 1, 1-dioxide (intermediate 18,0.849g, 2.298mmol, 1.8eq) in DCM (20ml) was added 2, 6-lutidine (0.32ml, 2.79mmol, 2.2eq) at 0 ℃. The reaction mixture was stirred at the same temperature for about 10 minutes, trifluoromethanesulfonic anhydride (0.43ml, 2.54mmol, 2.0eq) was added and stirred at 0 ℃ for about 15 minutes. Acetic acid (1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3 a-amino-5 a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-hydro-1H-cyclopenta [ a ] a]-9-Yl ester (example 20-step 2,0.600g, 1.277mmol, 1.0eq) and triethylamine (0.56ml, 4.06mmol, 3.5eq) were added to the reaction mixture, stirred at 0 ℃ for 1 hour and at room temperature overnight. TLC indicated that starting material had been consumed and the desired product was observed. The reaction mixture was basified with aqueous sodium bicarbonate and extracted with DCM (3 × 50 ml). The combined organic layers were washed with water (30ml), dried over sodium sulfate, filtered and concentrated under reduced pressure. Another 500mg batch with the same experimental procedure was combined and purified by silica gel column chromatography using 40-50% ethyl acetateGradient/hexanes to give the title compound (1.0g, 95.33% yield) as a solid.¹H NMR(300MHz,CDCl₃)：δppm 4.69(s,1H),4.59(s,1H),4.49-4.44(m,1H),3.66-3.53(m,13H),3.45-3.35(m,2H),3.38(s,3H),3.07-2.97(m,6H),2.04(s,3H),1.90-0.77(m,31H),1.68(s,3H),1.05(s,3H),0.95(s,3H),0.85(s,3H),0.84(s,3H),0.83(s,3H)。

Step 2: synthesis of 4- ((12R,13R) -14- (((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -9-hydroxy-5 a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-hydro-3 aH-cyclopenta [ a]3 a-yl) amino) -12-methyl-2, 5,8, 11-tetraoxatetradecan-13-yl) thiomorpholine 1, 1-dioxide:

to acetic acid (1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((12R,13R) -13- (1, 1-thiomorpholino) -12-methyl-2, 5,8, 11-tetraoxatetradecan-14-yl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-hydro-1H-cyclopenta [ a ] a]To a stirred solution of the-9-yl ester (step 1, 1.0g, 1.217mmol, 1.0eq) in methanol (10ml) and THF (10ml) was added 2.5N aqueous KOH (3.57ml, 9.13mmol, 7.5 eq). The reaction mixture was stirred at room temperature overnight. TLC indicated that the starting material was complete and the desired product was observed. The reaction mixture was evaporated under reduced pressure, diluted with water (40ml) and neutralized with 1N HCl solution and extracted with DCM (3 × 50 ml). The combined organic layers were washed with water (30ml), dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude compound was purified by column chromatography on silica gel using a 0-3% methanol/dichloromethane gradient to give the title compound (0.500g, 52.74% yield) as an off-white solid.¹H NMR(300MHz,CDCl₃)：δppm 4.69(s,1H),4.58(s,1H),3.66-3.53(m,14H),3.38(s,3H),3.30-3.0(m,8H),2.58-2.56(m,1H),2.41(m,1H),2.0-0.75(m,44H),1.67(s,3H)。

And step 3: synthesis of (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((12R,13R) -13- (1, 1-thiomorpholino) -12-methyl-2, 5,8, 11-tetraoxatetradecan-14-yl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-hydro-1H-cyclopenta [ a]-9-yl) ester:

to 4- ((12R,13R) -14- (((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -9-hydroxy-5 a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosahydro-3 aH-cyclopenta [ a]-3 a-yl) amino) -12-methyl-2, 5,8, 11-tetraoxatetradec-13-yl) thiomorpholine 1, 1-dioxide (step 2, 0.500g, 0.641mmol, 1.0eq) to a stirred solution in DCM (20ml) was added (1S,3R) -3- ((benzyloxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid (prepared according to the procedure described in WO2011/007230a2, 0.505g, 1.925mmol, 3.0eq) followed by edc.hcl (0.733g, 3.846mmol, 6.0eq) and DMAP (0.070g, 0.577mmol, 0.9 eq). The reaction mixture was stirred at room temperature overnight. TLC indicated that the starting material was complete and the desired product was observed. The reaction mixture was diluted with water (30ml) and extracted with DCM (3 × 30 ml). The combined organic layers were washed with water (30ml), dried over sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified by column chromatography on silica gel using a 0-3% methanol/dichloromethane gradient to give the title compound (0.060g, 22.8% yield) as a solid.¹H NMR(300MHz,CDCl₃)：δppm 7.34(m,5H),5.14,5.09(ABq,J_AB＝12.3Hz,2H),4.69(s,1H),4.58(s,1H),4.46-4.40(m,1H),4.06-4.0(m,1H),3.78-3.52(m,13H),3.37(s,5H),3.07-2.97(m,6H),2.84-2.52(m,5H),2.42-2.38(m,1H),2.08-0.77(m,31H),1.68(s,3H),1.33(s,3H),1.04(s,3H),0.96(s,3H),0.95(s,3H),0.84(s,3H),0.83(s,3H)。

And 4, step 4: synthesis of (1R,3S) -3- ((((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((12R,13R) -13- (1, 1-thiomorpholino) -12-methyl-2, 5,8, 11-tetraoxatetradecan-14-yl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosahydro-1H-cyclopenta [ a]9-yl) oxy) carbonyl) -2, 2-dimethylcyclobutane-1-carboxylic acid:

to (1R,3S) -2, 2-dimethylcyclobutane-1, 3-dicarboxylic acid 1-benzyl 3- ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR) -3a- (((12R,13R) -13- (1, 1-thiomorpholino) -12-methyl-2, 5,8, 11-tetraoxatetradecan-14-yl) amino) -5a,5b,8,8,11 a-pentamethyl-1- (prop-1-en-2-yl) eicosa-hydro-1H-cyclopenta [ a]To a stirred solution of (9-yl) ester (step 3, 0.200g, 0.195mmol, 1.0eq) in THF (2ml) and methanol (2ml) was added 1.5N aqueous KOH (0.97ml, 1.46mmol, 7.5 eq). The reaction mixture was stirred at room temperature for 2 days. TLC indicated that the starting material was complete and the desired product was observed. The reaction mixture was evaporated under reduced pressure, diluted with water (10ml), acidified to pH 4 to 5 with 1N HCl and extracted with DCM (3 × 50 ml). The combined organic layers were washed with water (30ml), dried over sodium sulfate, filtered and concentrated under reduced pressure. The crude compound was purified by silica gel column chromatography using a 0-5% methanol/dichloromethane gradient. The resulting compound was treated with ethyl acetate (2ml) and hexane (8ml), stirred at room temperature for 20 minutes, the solvent decanted off, the process repeated twice, finally filtered, washed with hexane (2ml) and dried under vacuum to give the title compound (0.060g, 32.96% yield) as a solid.¹H NMR(300MHz,CDCl₃)：δppm 4.68(s,1H),4.59(s,1H),4.54(m,1H),3.80-3.52(m,12H),3.37(s,3H),3.27-2.32(m,16H),2.03-0.77(m,25H),1.68(s,3H),1.38(s,3H),1.18(s,3H),1.06(s,3H),0.98(s,3H),0.92(s,3H),0.87(s,6H),0.83(s,3H)；ES-MS：m/z 933.94(M+H)⁺。

Biological activity

The compounds described herein can be tested for antiviral activity according to procedures known to those of ordinary skill in the art. For example, the following protocol can be employed to test compounds. These schemes are illustrative and are not limiting to the scope of the invention.

Example 23: evaluation of antiviral activity of compounds against HIV-1 strain 92HT599 in MT2 cells:

MT2 cells (10TCID 50/30000 cells) were infected with HIV-1 strain 92HT 599. Infected cells were plated in 96-well plates at a concentration of about 30,000 cells per well. Test compounds were added to the microplates in a prescribed form such that the final concentration of DMSO (vehicle) did not exceed 1%. In CO₂Incubate for about 96 hours in an incubator for viral infection. At the end of the incubation period, aliquots from each well were taken for p24 evaluation. Quantification of p24 is an indicator of antiviral activity of the compound. Percent inhibition was calculated with reference to control values (vehicle control). P24 evaluation was performed using the Advance biosciences kit according to the procedure specified by the supplier.

For serum binding assays, wherein "A" refers to IC₅₀Values less than 10nM, "B" means IC₅₀Values in the range of 10.01-20nM, and "C" refers to IC₅₀Values were greater than 20 nM. IC (integrated circuit)₅₀The (nM) values are listed in Table 1 below.

Table 1:

example 24: evaluation of antiviral activity of the compounds against the pNL4-3/WT and V7A strains in MT4 cells:

MT4 cells were transfected with HIV-1 plasmids (pNL4-3-WT and V7A) (cells were transfected with the required number of HIV-1 Ts at 37 ℃CID50 incubation for 1.5 hours). After infection, infected cells were plated at 3X10 per well⁴The concentration of individual cells was plated in 96-well plates. Test compounds are added to the test plate in a prescribed form such that the final concentration of DMSO does not exceed 1%. In CO₂Incubate for 4 days in the incubator for viral infection. At the end of the incubation period, aliquots from each well were taken for p24 evaluation. P24 evaluation was performed using the Advance biosciences kit according to the procedure specified by the supplier.

For the pNL4-3 WT assay, the selected compounds showed IC₅₀Wherein "A" refers to an IC of less than 10nM₅₀The value "B" means an IC in the range of 10.01-50nM₅₀A value, and "C" means IC₅₀Values greater than 50 nM;

for the pNL 4-3V 7A assay, the selected compounds showed IC₅₀Wherein "A" refers to an IC of less than 1-50nM₅₀The value "B" means an IC in the range of 50.01-100nM₅₀A value, and "C" means IC₅₀Values greater than 100 nM. IC (integrated circuit)₅₀The (nM) values are given in Table 2.

TABLE 2

Reference documents:

1.Antiviral methods and protocols(Eds：D Kinchington and R.F.Schinazi)Humana Press Inc.,2000.

2.HIV protocols(Eds：N.L.Michael and J.H.Kim)Humana Press Inc,1999.

3.DAIDS Virology manual from HIV laboratories,Publication NIH-97-3838,1997.

4.HIV-1p24 antigen capture assay,enzyme immunoassay for detection of Human immunodeficiency Virus Type 1(HIV-1)p24 in tissue culture media-Advanced bio science laboratories,Inc kit procedure.

although the invention herein has been described with reference to particular embodiments, it is to be understood that these embodiments are merely illustrative of the principles and applications of the present invention. It is therefore to be understood that numerous modifications may be made to the illustrative embodiments and that other arrangements may be devised without departing from the spirit and scope of the present invention as described above.

All publications and patent applications cited in this application are herein incorporated by reference to the same extent as if each individual publication or patent application were specifically and individually indicated to be incorporated by reference.

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