阅读说明：本技术 带上清液收集装置的离心管及其使用方法 (Centrifuge tube with supernatant collecting device and using method thereof ) 是由 康维君 庄伟强 茅富燕 刘伟 于 2020-05-27 设计创作，主要内容包括：一种带上清液收集装置的离心管及其使用方法,包括：离心管、上清液收集装置,其特征在于所述离心管为圆柱形管体,一端封闭,另一端的上部管壁上设有一出液口,上清液收集装置为一与离心管直径配合的圆柱体的推棒,该推棒圆柱体的外壁一侧设有一条凹槽,凹槽从底部延伸至柱体的上部,推棒嵌套在离心管内,推棒外壁与离心管内壁为密封动配合。本发明的优点是可以减少上清液收集过程中的人为误差,提高实验结果的重复性和准确性,其结构简单,使用方便,成本低廉,避免对离心后样品的扰动,依需要收取上清液的容量,并可直接对样品在离心管中密封保存,适合实验室中对离心后分层的上清液进行收集。(A centrifuge tube with a supernatant collecting device and a using method thereof comprise: centrifuging tube, supernatant collection device, its characterized in that the centrifuging tube is cylindrical body, and one end is sealed, is equipped with a liquid outlet on the upper portion pipe wall of the other end, and supernatant collection device be one with the push rod of centrifuging tube diameter complex cylinder, this push rod cylinder's outer wall one side is equipped with a recess, the recess extends to the upper portion of cylinder from the bottom, the push rod nestification is in the centrifuging tube, the push rod outer wall is sealed movable fit with the centrifuging tube inner wall. The invention has the advantages of reducing human errors in the process of collecting the supernatant, improving the repeatability and accuracy of experimental results, having simple structure, convenient use and low cost, avoiding disturbance to the centrifuged sample, collecting the volume of the supernatant according to the requirement, directly sealing and storing the sample in a centrifugal tube, and being suitable for collecting the supernatant which is layered after centrifugation in a laboratory.)

1. A centrifugal tube with a supernatant collecting device comprises a centrifugal tube and the supernatant collecting device, and is characterized in that the centrifugal tube is a cylindrical tube body, one end of the centrifugal tube is closed, a liquid outlet is formed in the upper tube wall of the other end of the centrifugal tube, the supernatant collecting device is a cylindrical push rod matched with the diameter of the centrifugal tube, a groove is formed in one side of the outer wall of the cylindrical push rod, the groove extends to the upper portion from the bottom of the push rod, the push rod is nested in the centrifugal tube, and the outer wall of the push rod is in sealing movable fit with the inner wall of the centrifugal tube.

2. The centrifuge tube with the supernatant collecting device according to claim 1, wherein the centrifuge tube has a diameter of 15-18 mm and a length of 100-120 mm, the liquid outlet is located 30-50 mm away from the mouth of the centrifuge tube, and the inner diameter of the liquid outlet is 2-4 mm.

3. The centrifuge tube with the supernatant collecting device of claim 1, wherein the outer diameter of the push rod is matched with the inner diameter of the centrifuge tube, the length of the push rod is 120-150 mm, the length of the groove on one side of the outer wall of the push rod is 60-80 mm, and the cross section of the groove is semicircular.

4. The centrifuge tube with a supernatant collecting device according to claim 1, wherein the centrifuge tube is made of transparent glass or plastic, the push rod is made of glass or plastic, and the inner wall surface of the centrifuge tube and the outer wall surface of the push rod are frosted.

5. The use method of the centrifuge tube with the supernatant collecting device is characterized by comprising the following steps:

A. a suspension to be centrifuged is filled in a centrifugal tube with a liquid outlet on the upper tube wall, and the liquid level of the suspension is lower than the liquid outlet;

B. centrifuging the suspension in the centrifugal tube;

C. after the centrifugation is finished, pushing a cylindrical push rod matched with the diameter of the centrifuge tube into the centrifuge tube, wherein one side of the outer wall of the cylinder on the push rod is provided with a groove, and the groove is aligned with a liquid outlet of the centrifuge tube and pushed into the centrifuge tube;

D. continuously pushing the push rod until the supernatant rises along the groove of the push rod and flows out through the liquid outlet of the centrifuge tube, and continuously pushing the push rod until the required supernatant sampling is finished;

E. and rotating the push rod to stagger the groove on the push rod and the liquid outlet of the centrifuge tube, so that the residual liquid in the centrifuge tube is sealed in the centrifuge tube, and the push rod is pulled out when the residual liquid needs to be centrifuged again or taken out.

Technical Field

The invention relates to test instrument equipment and a using method thereof, in particular to a centrifuge tube with a supernatant collecting device and a using method thereof.

Background

At present, in many chemical analysis tests, pretreatment of a sample is often required. The method is a common pretreatment method, wherein the extracting solution is added into a sample, fully mixed and then centrifuged, so that the sample solution is layered. The supernatant needs to be collected, and sometimes the same sample needs to be extracted for multiple times, and the supernatant needs to be collected for multiple times. The common method for collecting the supernatant is to directly suck the supernatant by using a dropper or a pipette. If the suction head is inserted too deeply, the lower-layer substances can be sucked, and the experimental result is influenced; if the insertion is too shallow, the liquid suction amount is insufficient, and repeated suction is needed; improper handling can also result in mixing of the upper and lower layers, requiring re-centrifugation. At present, the actual operation process needs to depend on the experience and proficiency of an operator, and a method capable of effectively controlling the artificial error is lacked.

Disclosure of Invention

The invention aims to provide a centrifuge tube with a supernatant collecting device and a using method thereof, aiming at the defects in the prior art. The device comprises a centrifugal tube and a supernatant collecting device, and is characterized in that the centrifugal tube is a cylindrical tube body, one end of the centrifugal tube is closed, a liquid outlet is formed in the upper tube wall of the other end of the centrifugal tube, the supernatant collecting device is a cylindrical push rod matched with the diameter of the centrifugal tube, a groove is formed in one side of the outer wall of the cylinder of the push rod, the groove extends from the bottom of the push rod to the upper part of the push rod, the push rod is nested in the centrifugal tube, and the outer wall of the push rod is in sealing movable fit with the inner wall of the centrifugal tube.

The diameter of centrifuging tube is at 15 ~ 18mm, and length is at 100 ~ 120mm, and the liquid outlet is located apart from centrifuging tube oral area 30 ~ 50mm department, and the internal diameter of liquid outlet is 2 ~ 4 mm.

The outer diameter of the push rod is matched with the inner diameter of the centrifugal tube, the length of the push rod is 120-150 mm, the length of a groove on one side of the outer wall of the push rod is 60-80 mm, and the cross section of the groove is semicircular.

The centrifugal tube is made of transparent glass or plastic, the push rod is made of glass or plastic, and the surface of the inner wall of the centrifugal tube and the surface of the outer wall of the push rod are frosted.

The use method of the centrifuge tube with the supernatant collecting device is characterized by comprising the following steps:

A. a suspension to be centrifuged is filled in a centrifugal tube with a liquid outlet on the upper tube wall, and the liquid level of the suspension is lower than the liquid outlet;

B. centrifuging the suspension in the centrifugal tube;

C. after the centrifugation is finished, pushing a cylindrical push rod matched with the diameter of the centrifuge tube into the centrifuge tube, wherein one side of the outer wall of the cylinder on the push rod is provided with a groove, and the groove is aligned with a liquid outlet of the centrifuge tube and pushed into the centrifuge tube;

D. continuously pushing the push rod until the supernatant rises along the groove of the push rod and flows out through the liquid outlet of the centrifuge tube, and continuously pushing the push rod until the required supernatant sampling is finished;

E. the push rod is rotated to enable the groove in the push rod to be staggered with the liquid outlet of the centrifuge tube, and the push rod can seal the top of the liquid level in the centrifuge tube due to the fact that the liquid level is flush with the bottom of the push rod, so that the residual liquid in the centrifuge tube is sealed in the centrifuge tube, and the push rod is pulled out when the residual liquid needs to be centrifuged again or taken out.

The invention has the advantages of reducing human errors in the process of collecting the supernatant, improving the repeatability and accuracy of experimental results, having simple structure, convenient use and low cost, avoiding disturbance to the centrifuged sample, collecting the volume of the supernatant according to the requirement, directly sealing and storing the sample in a centrifugal tube, and being suitable for collecting the supernatant which is layered after centrifugation in a laboratory.

Drawings

FIG. 1 is a schematic cross-sectional structural view of the present invention;

FIG. 2 is a schematic top view of the cross-sectional structure of the present invention.

In the figure: 1 liquid outlet, 2 centrifuging tubes, 3 grooves, 4 push rods.

Detailed Description

Embodiments of the invention are further described below with reference to the accompanying drawings:

referring to fig. 1 and 2, the centrifugal tube 2 is made of transparent glass or plastic, and the centrifugal tube 2 of this embodiment is made of glass and is a cylindrical tube body, similar to a test tube. The inner wall surface of centrifuging tube 2 is dull polish form, and the body one end of centrifuging tube 2 is sealed, is equipped with a liquid outlet 1 on the upper portion pipe wall of the other end. The diameter of centrifuging tube 2 is at 15 ~ 18mm, and length is at 100 ~ 120mm, and the liquid outlet is located apart from centrifuging tube oral area 30 ~ 50mm department, and the internal diameter of liquid outlet is 2 ~ 4 mm. The diameter of this embodiment centrifuging tube is 16mm, and length is 110mm, and liquid outlet 1 is located apart from centrifuging tube mouth portion 45mm department, and the internal diameter of liquid outlet is 3 mm. The supernatant collecting device is a push rod 4, the push rod 4 is made of glass or plastic, the push rod 4 of the embodiment is a cylinder which is made of glass and matched with the diameter of the centrifuge tube 2, the outer diameter of the push rod 4 is matched with the inner diameter of the centrifuge tube 4, the length is 120-150 mm, the length of the push rod 4 of the embodiment is 130mm, and the embodiment is 70 mm. One side of the outer wall of the push rod 4 is provided with a groove 3, the cross section of the groove 3 is semicircular, the length of the groove 3 is 60-80 mm, and the length of the embodiment is 70 mm. The inner wall surface of the centrifuge tube 2 and the outer wall surface of the push rod 4 are both frosted and are in close contact. The push rod is nested in the centrifugal tube, and the outer wall of the push rod is in sealing movable fit with the inner wall of the centrifugal tube.

The application method of the embodiment is carried out according to the following steps:

A. a suspension to be centrifuged is filled in a centrifugal tube 2 provided with a liquid outlet 1 on the upper tube wall, and the liquid level of the suspension is lower than the liquid outlet 1;

B. centrifuging the suspension in the centrifuge tube 2;

C. after centrifugation, pushing a cylindrical push rod 4 matched with the diameter of the centrifuge tube into the centrifuge tube 2, wherein one side of the outer wall of the cylinder on the push rod 4 is provided with a groove 3, aligning the groove 3 on the push rod 4 with a liquid outlet 1 and pushing the push rod 4 into the centrifuge tube 2;

D. continuously pushing the push rod 4, enabling the supernatant to ascend along the groove 3 of the push rod 4 and flow out through the liquid outlet 1 of the centrifuge tube 2 along with the continuous pressing of the push rod 4, then continuously pushing the push rod 4, observing the change of an interface when the bottom of the push rod 4 is close to the interface of upper and lower layers, and controlling the insertion degree of the push rod until the required supernatant is sampled and the pushing is stopped;

E. rotatory push rod 4, recess 3 on the push rod 4 staggers with the liquid outlet 1 of centrifuging tube 2, because the bottom parallel and level of liquid level and push rod 4, push rod 4 can be sealed in centrifuging tube 2 with the liquid level top in centrifuging tube 2, and the sample after centrifugal separation is sealed in centrifuging tube 2 by centrifuging tube 2 and push rod 4 with surplus liquid promptly, reaches the effect that directly seals up the save to the sample. If the liquid needs to be centrifuged again or the residual liquid needs to be taken out, the push rod can be pulled out.