阅读说明：本技术 新型冠狀病毒居家快速检测装置 (Novel crown virus house fast detection device ) 是由 陆培华 刘朝煜 邹健 邵君飞 于 2021-09-26 设计创作，主要内容包括：本发明涉及病毒检测技术领域,尤其涉及新型冠狀病毒居家快速检测装置,包括装置主体,安装在装置主体前端的显示屏,所述装置主体的一侧固定安装有连接壳,所述连接壳内壁上设置有识别机构,所述识别机构包括固定安装在连接壳内壁上的托架、从连接壳后端面活动插入且位于托架上端面的生物芯片,所述连接壳的内侧顶面上设置有可升降调节的摄像机构,所述连接壳的内壁上靠近识别机构的上方设置有可前后调节的紫外机构,本发明可以很好的运用在CRISPR技术与等温扩增技术的相结合上,可实现核酸序列的高效识别、信号的快速转换和高保真放大,同时大幅降低对仪器设备、检测环境及人员操作的依赖,从而达到快速、高效、灵敏地检测病毒的目的。(The invention relates to the technical field of virus detection, in particular to a novel crown virus household rapid detection device which comprises a device body and a display screen arranged at the front end of the device body, wherein a connecting shell is fixedly arranged at one side of the device body, an identification mechanism is arranged on the inner wall of the connecting shell, the identification mechanism comprises a bracket fixedly arranged on the inner wall of the connecting shell, and a biochip movably inserted from the rear end face of the connecting shell and positioned on the upper end face of the bracket, a camera shooting mechanism capable of being adjusted in a lifting manner is arranged on the top face of the inner side of the connecting shell, and an ultraviolet mechanism capable of being adjusted back and forth is arranged on the inner wall of the connecting shell close to the upper part of the identification mechanism The dependence of environment and personnel operation is detected, thereby achieving the purpose of quickly, efficiently and sensitively detecting the virus.)

1. Novel quick detection device of corona virus at home, including device main part (1), install display screen (2) at device main part (1) front end, its characterized in that: one side fixed mounting of device main part (1) has connection shell (3), be provided with recognition mechanism (4) on connection shell (3) inner wall, recognition mechanism (4) include bracket (42) of fixed mounting on connection shell (3) inner wall, insert and be located biochip (41) of bracket (42) up end from connection shell (3) rear end face activity, be provided with camera shooting mechanism (6) of liftable regulation on the inboard top surface of connection shell (3), the top that is close to recognition mechanism (4) on the inner wall of connection shell (3) is provided with ultraviolet mechanism (7) that can adjust from beginning to end, the lower extreme of connection shell (3) opens in the outside, just the lower extreme cartridge of connection shell (3) has end seal mechanism (5).

2. The device for detecting a corona virus at home as claimed in claim 1, further comprising: the lower terminal surface fixed mounting of bracket (42) has mount (43), the both sides limit of mount (43) is followed and is extended downwards, just the both sides surface of mount (43) respectively with the inner wall fixed connection of connecting shell (3).

3. The device for detecting a corona virus at home as claimed in claim 1, further comprising: end seal mechanism (5) include from connecting shell (3) the open concave shell (51) that the department card was gone into of lower extreme, the both sides surface elastic connection of concave shell (51) has card strip (54), draw-in groove (55) have been seted up to the position department that the inner wall of connecting shell (3) corresponds card strip (54), card strip (54) activity card is gone into the inboard of draw-in groove (55), be close to lower extreme department fixedly connected with and draw and drag board (52) between the both sides inner wall of concave shell (51).

4. The device for detecting a corona virus at home as claimed in claim 3, further comprising: the side cross-sectional shape of card strip (54) and draw-in groove (55) is triangle-shaped, the surface embedding of concave shell (51) has round elastic ring (53), the surface of the both sides surface of elastic ring (53) fixed connection card strip (54) respectively, elastic ring (53) are the rubber material.

5. The device for detecting a corona virus at home as claimed in claim 1, further comprising: camera shooting mechanism (6) are including setting up camera (61), the connecting plate (62) of fixed connection in camera (61) upper end connecting shell (3) inboard, be provided with on the both sides border of connecting plate (62) buckle limit (63), and both sides buckle limit (63) respectively with connect the shell (3) between the both sides wall activity laminating, the up end of connecting plate (62) is rotated and is connected with threaded rod (65), just the up end of threaded rod (65) is passed the up end of connecting shell (3) and is screw-thread fit each other.

6. The new crown virus home rapid detecting device as claimed in claim 5, wherein: the upper end face of the connecting shell (3) is fixedly embedded with a threaded cylinder (64), and the threaded rod (65) penetrates through the inner side of the threaded cylinder (64) and is in threaded fit with the threaded cylinder.

7. The new crown virus home rapid detecting device as claimed in claim 5, wherein: the lower extreme fixedly connected with card of threaded rod (65) is gone into dish (67), the outside activity cover of threaded rod (65) is equipped with pressure disk (66), just the border fixed connection of pressure disk (66) is at the up end of connecting plate (62), the inboard top surface of pressure disk (66) is laminated with the up end activity of card income dish (67), just the lower terminal surface of card income dish (67) is laminated with the up end activity of connecting plate (62).

8. The device for detecting a corona virus at home as claimed in claim 1, further comprising: ultraviolet mechanism (7) are including slide bar (71), two lamp bodies (74) of fixed suit at slide bar (71) surface that extend along both sides direction, two lamp body (74) are the symmetry and set up, slide rail (75) towards the fore-and-aft direction extension are seted up respectively to the both sides surface of connecting shell (3), the both ends of slide bar (71) slide the inboard of passing slide rail (75) respectively, the both ends of slide bar (71) respectively with be provided with locking mechanism between the both sides surface of connecting shell (3).

9. The new crown virus home rapid detecting device as claimed in claim 8, further comprising: locking mechanism establishes lantern ring (73) at the slide bar (71) other end including fixed disk (72), the cover of fixed connection in slide bar (71) one end, lantern ring (73) and fixed disk (72) are located the both sides surface of connecting shell (3) respectively, screw-thread fit between lantern ring (73) and slide bar (71), fixed disk (72) support is at the one side surface of connecting shell (3).

Technical Field

The invention relates to the technical field of virus detection, in particular to a novel device for quickly detecting a corona virus at home.

Background

The CRISPR is a repetitive sequence in a prokaryotic genome, is an immune weapon generated by the struggle of bacteria and viruses in the life evolution history, namely simply, the viruses can integrate own genes into the bacteria, a cell tool of the bacteria is utilized to provide a service for the gene replication of the bacteria, the bacteria evolve a CRISPR-Cas9 system in order to eliminate the foreign invasion genes of the viruses, and by utilizing the system, the bacteria can excise the virus genes from the genome of the bacteria without fixing the sound, which is a specific immune system of the bacteria and is an acquired immune system of archaea and the bacteria for resisting the invasion of foreign genetic materials such as the viruses and the like;

the isothermal amplification technology is a novel nucleic acid detection technology which combines a new generation of nucleic acid isothermal amplification technology and a real-time fluorescence detection technology;

the nucleic acid detection based on the fluorescence quantitative PCR is mainly used for diagnosing the coronavirus pneumonia at present, a special molecular laboratory is needed, the requirements on instruments and operators are high, the integral detection time is long, the accuracy is insufficient, the false negative rate is high, and therefore a portable nucleic acid detection device which is high in sensitivity, easy to operate and short in detection time is needed, is used for application scenes of personnel dispersion and laboratory infrastructure shortage, and can effectively improve epidemic situation prevention and control efficiency.

Disclosure of Invention

The invention aims to solve the defects in the background technology and provides a novel device for quickly detecting the corona virus at home.

In order to achieve the above purposes, the technical scheme adopted by the invention is as follows: novel corona virus is quick detection device at home, including the device main part, install the display screen at device main part front end, one side fixed mounting of device main part has the connection shell, be provided with identification mechanism on the connection shell inner wall, identification mechanism includes bracket fixed mounting on the connection shell inner wall, inserts and is located the biochip of bracket up end from connection shell rear end face activity, be provided with the mechanism of making a video recording of liftable regulation on the inboard top surface of connection shell, the top that is close to identification mechanism on the inner wall of connection shell is provided with the ultraviolet mechanism that can adjust from beginning to end, the lower extreme cartridge of connection shell opens in the outside, just the lower extreme cartridge of connection shell has bottom sealing mechanism.

Preferably, a fixing frame is fixedly mounted on the lower end face of the bracket, two side edges of the fixing frame extend downwards, and the outer surfaces of two sides of the fixing frame are fixedly connected with the inner wall of the connecting shell respectively.

Preferably, the end sealing mechanism includes the concave shell of following the open department card income of connecting the shell lower extreme, the both sides surface elastic connection of concave shell has the card strip, the position department that the inner wall of connecting the shell corresponds the card strip has seted up the draw-in groove, the inboard of draw-in groove is gone into to card strip activity card, be close to lower extreme department fixedly connected with between the both sides inner wall of concave shell and draw the board.

Preferably, the side cross-sectional shapes of the clamping strip and the clamping groove are both triangular, a circle of elastic ring is embedded into the outer surface of the concave shell, the outer surfaces of two sides of the elastic ring are fixedly connected with the outer surface of the clamping strip respectively, and the elastic ring is made of rubber.

Preferably, the camera shooting mechanism is including setting up at the inboard camera of connecting shell, the connecting plate of fixed connection in the camera upper end, be provided with the limit of buckling on the both sides border of connecting plate, and both sides buckle the limit respectively with the both sides wall of connecting shell between the activity laminating, the up end of connecting plate rotates and is connected with the threaded rod, just the up end of connecting shell and the screw-thread fit each other are passed to the upper end of threaded rod.

Preferably, a threaded cylinder is fixedly embedded in the upper end face of the connecting shell, and the threaded rod penetrates through the inner side of the threaded cylinder and is in threaded fit with the threaded cylinder.

Preferably, the lower extreme fixedly connected with card of threaded rod goes into the dish, the outside activity cover of threaded rod is equipped with the pressure disk, just the border fixed connection of pressure disk is at the up end of connecting plate, the inboard top surface of pressure disk and the up end activity laminating of card income dish, just the lower terminal surface of card income dish and the up end activity laminating of connecting plate.

Preferably, the ultraviolet mechanism includes the slide bar that extends along both sides direction, two lamp bodies of fixed suit at the slide bar surface, two the lamp body is the symmetry setting, the slide rail that extends towards the fore-and-aft direction is seted up respectively to the both sides surface of connecting the shell, the both ends of slide bar slide the inboard of passing the slide rail respectively, the both ends of slide bar respectively with be provided with locking mechanism between the both sides surface of connecting the shell.

Preferably, locking mechanism includes fixed disk, the cover of establishing at the slide bar other end of fixed connection in slide bar one end and establishes the lantern ring, the lantern ring is located the both sides surface of connecting the shell respectively with the fixed disk, screw-thread fit between lantern ring and the slide bar, the fixed disk supports the surface in one side of connecting the shell.

Compared with the prior art, the invention has the following beneficial effects:

1. the invention can be well applied to the combination of the CRISPR technology and the isothermal amplification technology, can realize the high-efficiency recognition of nucleic acid sequences, the quick conversion of signals and the high-fidelity amplification, and greatly reduces the dependence on instruments, detection environments and personnel operation, thereby achieving the aim of quickly, efficiently and sensitively detecting viruses.

2. Extracting nucleic acid from a blood sample or a throat swab sample, carrying out PCR isothermal amplification on the nucleic acid to obtain a PCR product, wherein the primer used for carrying out the PCR amplification is a biotinylated primer, adding the PCR product obtained after the PCR amplification onto a biochip, carrying out nucleic acid hybridization by using different hybridization probes for spotting, simultaneously dripping a marker liquid onto the biochip to enable the PCR product obtained after the nucleic acid hybridization to react with the marker liquid, then inserting the biochip onto the surface of a bracket, and capturing an image by a camera mechanism; if the detected virus is positive, the image contains a quantum dot fluorescence image for representing the virus, and then the virus is contained.

3. Through rotating the threaded rod for the threaded rod reciprocates, and then drives the connecting plate and reciprocates, thereby adjusts the camera to the distance between the bracket, and to the range of adjustment capture image that different biochips can be nimble, adaptability is good.

4. Through loosening the lantern ring soon, can slide the slide bar along the inboard of slide rail, adjust the position of shining of lamp body, the lamp body shines the quantum dot on the biochip and in order to arouse and produce fluorescence signal, also can rotate the slide bar, adjusts the angle of shining of lamp body, uses in a flexible way.

5. Exert external force and pull out downwards and draw and drag the board for the inner wall of card strip and draw-in groove pushes away each other, thereby extrudees the elastic ring, and the card strip is absorbed in concave type shell inboardly, thereby ensures that concave type shell can be pulled out, easily disassembles, thereby is convenient for clean inboard dust.

Drawings

FIG. 1 is a schematic structural view of the novel corona virus household rapid detection device of the present invention;

FIG. 2 is a cross-sectional view of the connecting shell of the novel corona virus household rapid detection device;

FIG. 3 is an enlarged view of the portion A of the fast household corona virus detection device of the present invention shown in FIG. 2;

FIG. 4 is another cross-sectional view of the connecting shell of the present invention, illustrating a corona virus household rapid detection device;

FIG. 5 is a schematic view of a camera mechanism of the present invention for fast detecting a corona virus at home;

FIG. 6 is a schematic view of an identification mechanism of the present invention for fast detecting a corona virus at home.

1. A device main body; 2. a display screen; 3. a connecting shell; 4. an identification mechanism; 41. a biochip; 42. a bracket; 43. a fixed mount; 5. a bottom sealing mechanism; 51. a concave shell; 52. a pulling plate; 53. an elastic ring; 54. clamping the strip; 55. a card slot; 6. a camera mechanism; 61. a camera; 62. a connecting plate; 63. bending the edges; 64. a threaded barrel; 65. a threaded rod; 66. a platen; 67. clamping into a disc; 7. an ultraviolet mechanism; 71. a slide bar; 72. fixing the disc; 73. a collar; 74. a lamp body; 75. a slide rail.

Detailed Description

The following description is presented to disclose the invention so as to enable any person skilled in the art to practice the invention. The preferred embodiments in the following description are given by way of example only, and other obvious variations will occur to those skilled in the art.

As shown in fig. 1-6 novel quick detection device of corona virus house, including device main part 1, install the display screen 2 at 1 front end of device main part, one side fixed mounting of device main part 1 has connection shell 3, be provided with identification mechanism 4 on the 3 inner walls of connection shell, identification mechanism 4 includes bracket 42 of fixed mounting on the 3 inner walls of connection shell, insert and be located the biochip 41 of bracket 42 up end from connecting 3 rear end face activities of shells, be provided with the mechanism 6 of making a video recording of liftable regulation on the inboard top surface of connection shell 3, the upper portion that is close to identification mechanism 4 on the inner wall of connection shell 3 is provided with ultraviolet mechanism 7 that can adjust from beginning to end, the lower extreme of connecting shell 3 opens in the outside, and the lower extreme cartridge of connecting shell 3 has end seal 5.

The lower end face of the bracket 42 is fixedly provided with a fixed mount 43, two side edges of the fixed mount 43 extend downwards, and the outer surfaces of two sides of the fixed mount 43 are respectively fixedly connected with the inner wall of the connecting shell 3, so that the stability of the bracket 42 is ensured.

End seal mechanism 5 includes from connecting the open concave type shell 51 that goes into of shell 3 lower extreme, the both sides surface elastic connection of concave type shell 51 has card strip 54, the position department that connects shell 3's inner wall correspondence card strip 54 has seted up draw-in groove 55, card strip 54 activity card is gone into the inboard of draw-in groove 55, be close to lower extreme department fixedly connected with and draw and drag board 52 within a definite time in concave type shell 51's the both sides, draw and drag board 52 person of facilitating the use and pull out concave type shell 51, concave type shell 51 is convenient for detach down, the person of facilitating the use cleans the inboard of connecting shell 3.

The lateral section shapes of the clamping strip 54 and the clamping groove 55 are both triangular, a circle of elastic ring 53 is embedded in the outer surface of the concave shell 51, the outer surfaces of two sides of the elastic ring 53 are fixedly connected with the outer surface of the clamping strip 54 respectively, the elastic ring 53 is made of rubber, and the elastic ring 53 gives acting force for resetting the clamping strip 54.

Camera mechanism 6 is including setting up at the inboard camera 61 of connecting shell 3, fixed connection is at connecting plate 62 of camera 61 upper end, be provided with the limit 63 of buckling on the both sides border of connecting plate 62, the limit 63 of buckling promotes the stationary degree when connecting plate 62 reciprocates, and the limit 63 of buckling of both sides respectively with the both sides wall of connecting shell 3 between the activity laminating, the up end of connecting plate 62 is rotated and is connected with threaded rod 65, and threaded rod 65's upper end is passed the up end of connecting shell 3 and is screw-thread fit each other, rotate threaded rod 65 and can make threaded rod 65 reciprocate.

The thread matching mode between the specific threaded rod 65 and the connecting shell 3 is as follows: the upper end surface of the connecting shell 3 is fixedly embedded with a thread cylinder 64, and a threaded rod 65 penetrates through the inner side of the thread cylinder 64 and is in threaded fit with each other.

The lower extreme fixedly connected with card of threaded rod 65 is gone into dish 67, and the outside activity cover of threaded rod 65 is equipped with pressure disk 66, and the border fixed connection of pressure disk 66 is at the up end of connecting plate 62, and the inboard top surface of pressure disk 66 and the up end activity laminating of card income dish 67, and the lower terminal surface of card income dish 67 and the up end activity laminating of connecting plate 62 ensure between threaded rod 65 and the connecting plate 62 can rotate relatively to both can not break away from each other.

The ultraviolet mechanism 7 includes a slide bar 71 extending along two side directions, and two lamp bodies 74 fixedly sleeved on the outer surface of the slide bar 71, the lamp bodies 74 are ultraviolet radiation lamps, the lamp bodies 74 irradiate quantum dots on the biochip 41 to excite the quantum dots to generate fluorescent signals, the two lamp bodies 74 are symmetrically arranged, the outer surfaces of two sides of the connecting shell 3 are respectively provided with slide rails 75 extending towards the front and back directions, two ends of the slide bar 71 respectively slide through the inner sides of the slide rails 75, and locking mechanisms are respectively arranged between two ends of the slide bar 71 and the outer surfaces of two sides of the connecting shell 3.

The locking mechanism comprises a fixed disc 72 fixedly connected to one end of the sliding rod 71 and a sleeve ring 73 sleeved on the other end of the sliding rod 71, the sleeve ring 73 and the fixed disc 72 are respectively positioned on the outer surfaces of two sides of the connecting shell 3, the sleeve ring 73 is in threaded fit with the sliding rod 71, the fixed disc 72 abuts against the outer surface of one side of the connecting shell 3 to play a role in limiting the sliding rod 71, and therefore the lamp body 74 is fixed after the position and the angle are adjusted.

The invention can be well applied to the combination of the CRISPR technology and the isothermal amplification technology, can realize the high-efficiency recognition of nucleic acid sequences, the quick conversion of signals and the high-fidelity amplification, and greatly reduces the dependence on instruments, detection environments and personnel operation, thereby achieving the aim of quickly, efficiently and sensitively detecting viruses.

When the kit is used, nucleic acid is extracted from a blood sample or a throat swab sample, PCR isothermal amplification is carried out on the nucleic acid to obtain a PCR product, the primer used for the PCR amplification is a biotinylated primer, the PCR product obtained after the PCR amplification is added to the biochip 41, different hybridization probes are used for spotting to carry out nucleic acid hybridization, meanwhile, a marker liquid is dripped onto the biochip 41, the PCR product obtained after the nucleic acid hybridization is reacted with the marker liquid, then the biochip 41 is inserted onto the surface of the bracket 42, and an image is captured by the camera mechanism 6; if the detected virus is positive, the image contains a quantum dot fluorescence image for representing the virus, and then the virus is contained.

The user rotates the threaded rod 65 to enable the threaded rod 65 to move up and down, and further drives the connecting plate 62 to move up and down, so that the distance between the camera 61 and the bracket 42 is adjusted, the range of captured images can be flexibly adjusted for different biochips 41, and the adaptability is good; the sliding rod 71 can slide along the inner side of the sliding rail 75 by loosening the lantern ring 73, the irradiation position of the lamp body 74 is adjusted, the lamp body 74 irradiates the quantum dots on the biochip 41 to excite and generate fluorescent signals, the sliding rod 71 can also be rotated, the irradiation angle of the lamp body 74 is adjusted, and the use is flexible; exert external force and pull and draw board 52 downwards for the inner wall of card strip 54 and draw-in groove 55 pushes away each other, thereby extrudees elastic ring 53, and card strip 54 is absorbed in concave shell 51 inboardly, thereby ensures that concave shell 51 can be pulled out, easily disassembles, thereby is convenient for clean inboard dust.

In the present invention, the terms "mounting," "connecting," "fixing," and the like are used in a broad sense, for example, "connecting" may be a fixed connection, a detachable connection, or an integral connection; may be directly connected or indirectly connected through an intermediate. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

In the description herein, the appearances of the phrases "one embodiment," "some embodiments," "a specific embodiment," or the like, in various places throughout this specification are not necessarily all referring to the same embodiment or example, but rather to the same embodiment or example. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are merely illustrative of the principles of the invention, but that various changes and modifications may be made without departing from the spirit and scope of the invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.