阅读说明：本技术 鉴定免疫调节基因的方法 (Method for identifying immunoregulatory genes ) 是由 托马斯·亨利 莫达希尔·乔杜里 莉蒂亚·维尼 于 2019-11-26 设计创作，主要内容包括：本文公开了用于鉴定免疫调节基因的方法。在一些实施方案中,该方法包括筛选候选基因,该方法包括：a)在多于一个免疫细胞中表达外源细胞受体或其功能部分；b)将i和ii引入所述多于一个免疫细胞中：i.指导多核酸或编码所述指导多核酸的核酸,其中所述指导多核酸靶向所述候选基因；和ii.外源核酸酶或编码所述外源核酸酶的核酸；从而产生包含所述候选基因中的基因组破坏的多于一个工程化免疫细胞；c)使所述多于一个工程化免疫细胞与表达所述外源细胞受体或其功能部分的同源抗原的多于一个细胞接触,从而进行体外测定；并且d)确定所述体外测定的读取。(Disclosed herein are methods for identifying immunomodulatory genes. In some embodiments, the method comprises screening for a candidate gene, the method comprising: a) expressing a foreign cellular receptor or functional part thereof in more than one immune cell; b) introducing i and ii into the more than one immune cell: i. a guide polynucleic acid or a nucleic acid encoding the guide polynucleic acid, wherein the guide polynucleic acid targets the candidate gene; an exogenous nuclease or a nucleic acid encoding the exogenous nuclease; thereby generating more than one engineered immune cell comprising a genomic disruption in the candidate gene; c) contacting the more than one engineered immune cells with more than one cell expressing a cognate antigen of the foreign cellular receptor or functional portion thereof, thereby performing an in vitro assay; and d) determining the reading of the in vitro assay.)