阅读说明：本技术 新飘香藤属植物及其制出方法 (New Pistacia plant and its preparation method ) 是由 山田将弘 见里朝也 渡边健宏 东锐明 堀川学 于 2016-10-14 设计创作，主要内容包括：本发明旨在提供至今无法制出的有新的色调的花色的新飘香藤属植物。从候选飘香藤属植物的花瓣提取类胡萝卜素色素,在该类胡萝卜素色素提取液中存在新叶黄素或其衍生物时,选定为亲本飘香藤属植物。(The present invention aims to provide a novel spatholobus plant having a new color tone which has not been produced so far. Extracting carotenoid pigment from petals of candidate Pterocarya plant, and selecting as parent Pterocarya plant when new xanthophyll or its derivative exists in the carotenoid pigment extractive solution.)

1. A method for producing a Pistacia plant having petals of flower color with-10 to 70 a-value and 0 to 80 b-value in CIE L a b color system, characterized by using:

09M111-1 deposited under number FERM BP-22298, or

A descendant thereof having a property of containing a novel xanthophyll or a derivative thereof as a carotenoid pigment in petals.

2. The method of claim 1, wherein the carotenoid pigment is: neolutein dimyristate, neolutein 3-O-myristate-3 '-O-palmitate, neolutein 3-O-palmitate-3' -O-myristate, neolutein dipalmitate, or combinations thereof.

3. The method of claim 1, wherein said progeny have a color shade of petals in the CIE L A B color system with a value of-10 to 70 and b value of 0 to 80.

4. The method according to claim 3, wherein a Pistacia plant having a color tone of petals with a-value to-0 to 30 and b-value to 0 to 5 is excluded from the progeny.

5. The method of claim 3 or 4, wherein the progeny have, in the CIE L a b color series:

a color tone of petals wherein a is-5 to 20 and b is 3 to 70, or

a value of 50 to 70, and b value of 5 to 70.

6. The method of claim 1, wherein said progeny contains greater than 0.02mg anthocyanin pigment per 1g fresh petals.

7. The method of claim 6, wherein the anthocyanin pigment is anthocyanidin.

[ technical field ] A method for producing a semiconductor device

The present invention relates to a novel spatholobus (Mandevilla) plant containing at least 1 or more carotenoid pigments in its petals, a method for selecting a parent spatholobus plant containing a carotenoid pigment in its petals, which can be hybridized with a spatholobus plant having white to pink to red petals, and a method for producing a spatholobus hybrid plant using the method for selecting the parent spatholobus plant.

[ background of the invention ]

The plant of the Genus piper (Genus mantoulla), also known as piper parvifolia (dipadenia), is a sprawling woody or erect herb belonging to the family oleander (family Apocynaceae), native to about 130 species in tropical america, for example, from mexico to argentina.

Horticulture, around 1868, pink flutter vines (Mandevilla x amabilis (syn. m. amonea)) were produced from the united kingdom using beautiful flutter vines (Mandevilla spleendens) as one parent (the other parent is unknown). In 1960, a plant obtained from a strain designated as m.amonea blooms in the united states, and this is "Alice du Pont" (japanese name "Rose Giant") widely distributed even at present (see non-patent documents 1 and 2 below).

As long as about 2000 years, there was no species circulating in horticulture, and there were no species selected mainly from "Alice du Pont" and its naturally mutated branches instead of the species or from the seedling obtained by giving up the receptive powder, or from yet unknown carthamus tinctorius (mantavilla sanderi) and its branches instead of or from the seedling obtained by giving up the receptive powder, or from wild species-like marsdenia albopictus (mantavilla laxa) (Chilean Jasmine) or borlivia bergamia (manthali borliviansis) (see patent document 1 below).

Sandeli flowers (strain) were sold in 1998 as "white" of a large recurrent type (Mandevilla amabilis x boliviansis 'Sunmandeho', variety registration application No. 8721) (see patent document 2 below).

Further, sandeli flowers (plants) sold the same major alternate type "pink" (Mandevilla hybrida 'sunmandes', variety registration application No. 14756) in 2003 (see patent document 4 below).

Sandeli flowers (plants) sold in 2005 the tendril type "Red" (Madevilla hybrida 'Sunmandemi') (see patent document 5 and variety registration application No. 15862 below), "tropical peach red" (Mandevilla hybrida 'Sunmandeomi') (see patent document 6 and variety registration application No. 15863 below), the tendril type "Pink" (Sunparapi) (variety registration application No. 24471), the tendril type "Red velvet" (Mandevilla 'Sunparanga') (see patent document 14 and variety registration application No. 23333 below), and the tendril type (overseas called "Presty type)" Sundville pretreata "(Mandevilla 'Sunparapro') (see patent document 15 below).

Sandeli flowers (plants) were sold in 2007 under the "Kagaku" trade name (Mandevilla hybrida 'Sunmandecrikin') (see patent document 7 and variety registration application No. 17409 below).

Sandeli flowers (plants) were sold in the same year as the early blossoming type "deep red" (Mandevilla hybrida 'Sunmandecrim') (see patent document 3 and variety registration application No. 14755 below).

"carmine" (Mandevilla hybrida 'Sunmandecripi') (see patent document 8 and variety registration application No. 23604 below) and "milk pink" (Mandevilla hybrida 'Sunparabra') (see patent document 9 and variety registration application No. 24470 below) are also sold by Sandeli flowers (plants) of early blossoming type.

The aforementioned "deep red king" and "deep red" of the daoyuan type are genuine red muscovy not found before, and as these new varieties are entered, the market is expanded, and competitive companies of sandeli flowers (plants) also begin to breed muscovy.

Patent document 10 (assignee, Syngenta partitionans AG) below describes a new variety called Mandevilla sanderi (Hemsl.) Woodson ' Fisrix Pinka ' obtained by mating a variety provided as ' Sundaville ' (registered trademark) Red ' and ' Moulin Rouge ' in france, which is the same as the "deep Red" type described in patent document 3, as a parent with a variety provided as ' My hair Lady ' TM described in patent document 1 as a parent.

Patent documents 11 to 13 (assignee, florequest pty.ltd., and Protected Plant activities pty.ltd.) describe a new species called Mandevilla hybrida 'giner' obtained by mating Mandevilla hybrida identified by the number X02.5 as a parent with Mandevilla hybrida 'dark red' described in patent document 3 as a parent.

[ Prior art documents ]

[ patent document ]

[ patent document 1] U.S. plant patent No. 9117

[ patent document 2] U.S. plant patent No. 11556 publication

[ patent document 3] U.S. Pat. No. 15539

[ patent document 4] U.S. plant patent No. 15202

[ patent document 5] U.S. plant patent No. 16449

[ patent document 6] U.S. Pat. No. 16363 of plant

[ patent document 7] U.S. Pat. No. 17736

[ patent document 8] U.S. plant patent No. 18578

[ patent document 9] U.S. plant patent No. 19649

[ patent document 10] U.S. plant patent No. 20644 publication

[ patent document 11] U.S. plant patent No. 20776

[ patent document 12] U.S. plant patent No. 20777

[ patent document 13] U.S. Pat. No. 20919

[ patent document 14] U.S. plant patent No. 20542

[ patent document 15] U.S. plant patent No. 19399

[ non-patent literature ]

[ Nonpatent document 1] The New York cosmetic Garden illuminated Encyclopedia of Horticulture, Vol.6, p.2129-2130, Garland Publishing, Inc. (1981)

[ non-patent document 2] Mbberley's Plant-book, Third Edition, p.520, Cambridge University Press (2008)

[ summary of the invention ]

[ technical problem to be solved by the invention ]

The varieties of the Pistacia plant sold in the past are only white, pink and red. There are wild species having yellow flower colors, and in recent years, although a variety having yellow flower colors (Opale Citrine) is sold, since these cannot be crossed with a variety having white, pink, or red flower colors, a hybrid plant having a yellow flower color as a mating parent has not been produced yet. Therefore, new color expression by crossing the variety of the yellow flower color with the conventional white to pink to red flower colors (in the CIE L a b color system, flower colors having an a value of 0 to 60 and b value of 0 to 40) has not been realized. The present invention addresses the problem of providing a novel spatholobus plant having a new color tone which has not been produced in the past.

[ technical means for solving problems ]

The present inventors obtained a system 09M111-1 having a specific color tone among the seedlings obtained by crossing a red variety and a white variety, and found that the petals contained a specific carotenoid pigment although the amount thereof was small. Surprisingly, when this pterocarpus plant having a specific carotenoid pigment is mated with a variety of a pterocarpus plant having a white, pink, or red color system, hybrid seeds are obtained, and thus, a hybrid plant having a new color tone, which has not been produced in the past, is successfully produced.

Specifically, the present invention is as follows.

[1] A Pistacia plant comprising at least 1 or more carotenoid pigments in its petals, wherein said carotenoid pigment is neolutein or a derivative thereof.

[2] [1] the Pterocarya plant according to the above, wherein the carotenoid pigment is neolutein dimyristate, neolutein 3-O-myristate-3 '-O-palmitate, neolutein 3-O-palmitate-3' -O-myristate, neolutein dipalmitate or a combination thereof.

[3] A Pistacia plant comprising at least 1 or more carotenoid pigments in its petals, wherein said carotenoid pigment is a compound corresponding to peak A, peak C or peak D in the chromatographic profiles shown in FIGS. 1, 2 and 5.

[4] The petal of the plant of the genus Pimpinella contains neolutein synthase.

[5] [1] to [4], wherein the rosette plant has a color of-10 to 70 a and 0 to 80 b in the CIE L A B color system.

[6] [5] the Pistacia plant, wherein in the CIE L A B color system, there are flower colors other than flower colors with a value of 0-30 a and b value of 0-5 b.

[7] [1] to [4], wherein the CIE L A B color system has a color of-5 to 20a and 3 to 70 b, or a color of 50 to 70 a and 5 to 70 b.

[8] [1] the Pimenta plant according to any one of [1] to [7], wherein the anthocyanin pigment is contained in an amount of 0.02mg or more per 1g of fresh petals.

[9] [8] the Drepara plant, wherein the anthocyanin pigment is anthocyanidin.

[10] The Pimpinella plant is 09M111-1 (preservation number: FERM BP-22298).

[11] Progeny of the Pterocarpus plant of any one of [1] to [10 ].

[12] [1] the vegetative propagation material, a part of a plant, a tissue or a cell of the plant of the genus Pimenta or its progeny according to any one of [1] to [10 ].

[13] [1] the cut flower of the Pterocarpus plant or its progeny or the processed product thereof according to any one of [1] to [10 ].

[14] A method for selecting a parent Pimpinella plant having a carotenoid pigment in its petals, which is capable of crossing Pimpinella plants having white to pink to red (colors having an a value of 0 to 60 and a b value of 0 to 40 in the CIE L A B color system),

when neolutein or a derivative thereof is present as a carotenoid pigment in petals of candidate bauhinia plants, the candidate bauhinia plants are selected as parent bauhinia plants.

[15] [14] the method according to any one of the above methods, wherein the carotenoid pigment is neolutein dimyristate, neolutein 3-O-myristate-3 '-O-palmitate, neolutein 3-O-palmitate-3' -O-myristate, neolutein dipalmitate, or a combination thereof.

[16] A method for selecting a parent Pimpinella plant having a carotenoid pigment in its petals, which is capable of crossing Pimpinella plants having white to pink to red (colors having an a value of 0 to 60 and a b value of 0 to 40 in the CIE L A B color system),

when a compound corresponding to peak a, peak C or peak D in the chromatogram shown in fig. 1, 2 and 5 is present as a carotenoid pigment in petals of candidate spatholobus, the candidate spatholobus is selected as a parent spatholobus.

[17] A method for selecting a parent bauhinia plant capable of crossing with bauhinia plants having white to pink to red petals (colors having an a value of 0 to 60 and a b value of 0 to 40 in the CIE L A B color system),

when new lutein synthase is present in petals of candidate sweethearts, the parent sweethearts is selected.

[18] [14] the method according to any one of [14] to [17], wherein the color tone of petals of the candidate Pistacia plant is measured in the CIE L a b color system, and the parent Pistacia plant is selected when a value is-10 to 70 and b value is 0 to 80.

[19] [18] the method according to any one of the above methods, wherein when the value a is 0 to 30 and the value b is 0 to 5, the parent Pimpinella plant is excluded from the selection.

[20] [14] the method according to any one of [17], wherein the color tone of petals of the candidate Pistacia plant is measured in the CIE L a b color system, and the parent Pistacia plant is selected when a value is-5 to 20 and b value is 3 to 70, or when a value is 50 to 70 and b value is 5 to 70.

[21] The method according to any one of [14] to [20], wherein the amount of the anthocyanin pigment in the petals of the candidate Kadsura plant is measured, and when the anthocyanin pigment is contained in an amount of 0.02mg or more per 1g of fresh petals, the candidate Kadsura plant is selected as the parent Kadsura plant.

[22] [21] the method according to any one of the above methods, wherein the anthocyanin pigment is anthocyanin.

[23] The method according to any one of [14] to [22], wherein 09M111-1 (accession number: FERM BP-22298) is selected as the parent Botrytis plant.

[24] A method of producing a plant of the genus amygdalus having petals of-10 to 70 a-value and 0 to 80 b-value in CIE L a-b-color system (e.g., yellow to apricot yellow to orange, particularly ivory, pale yellow, apricot yellow, orange pink, orange red, orange), comprising:

a step of selecting a 1 st parent plant of the genus Pimpinella by the method according to any one of [14] to [23], and a step of crossing the selected 1 st parent plant of the genus Pimpinella and the selected 2 nd parent plant of the genus Pimpinella.

[25] [24] the method according to any one of [2] the parent plant of the genus Pimpinella, wherein the parent plant has petals of white to pink to red colors (flower colors having an a value of 0 to 60 and a b value of 0 to 40 in the CIE L A B color system).

[ Effect of the invention ]

The present invention makes it possible to produce a new spatholobus plant having petals of a new color tone that has not been conventionally available by crossing a spatholobus plant variety having a white to pink to red color system flower color, which has not been hybridized so far, with a variety having a yellow pigment.

[ description of the drawings ]

FIG. 1 is a chromatogram of HPLC-PDA (450nm) of acetone extract of petals of the Pistacia plant (4990) of the present invention.

FIG. 2 is a chromatogram of HPLC-PDA (450nm) of the acetone extract from petals of the Pistacia plant (09M111-1) of the present invention.

FIG. 3 is a chromatogram of conventional HPLC-PDA (450nm) of acetone extract from petals of Drepara plant (Opale Citrine).

FIG. 4 is the spectrocolorimeter data of the flower color of the Pterocarpus species of the present invention and the existing Pterocarpus species.

FIG. 5 shows the HPLC-PDA (450nm) chromatograms of the Pistacia plant (09M111-1) and the existing Pistacia plant (Opale Citrine) of the present invention.

FIG. 6 is an apparent absorption spectrum of a peak observed in an HPLC-PDA (450nm) chromatogram of the Pterocarpus plant (09M111-1) of the present invention.

FIG. 7 shows a Pistacia plant (09M111-1) of the present invention¹H-NMR spectrum (5.3-6.9 ppm).

FIG. 8 is a drawing of an existing Dria plant (Opale Citrine)¹H-NMR spectrum (5.3-6.9 ppm).

FIG. 9 shows a high decomposition energy mass extraction ion chromatogram of the Pistacia plant (09M111-1) of the present invention ((a) M/z 1021.81-1021.83, (b) M/z 1049.84-1049.86, and (c) M/z 1077.87-1077.89).

FIG. 10 is a high decomposition energy mass extraction ion chromatogram of an existing Drynariae plant (Opale Citrine) ((a) m/z 1021.81-1021.83, (b) m/z 1049.84-1049.86, and (c) m/z 1077.87-1077.89).

FIG. 11 is a high decomposition energy mass extraction ion chromatogram of an existing Drynariae plant (Opale Citrine) ((a) m/z 1005.82-1005.83, (b) m/z 1033.85-1033.87, and (c) m/z 1061.88-1061.90).

[ embodiment ] A method for producing a semiconductor device

In embodiment 1 of the present invention, there is provided a pianist plant having at least 1 or more carotenoid pigments in its petals, wherein the carotenoid pigment is a compound corresponding to peak a, peak C or peak D in the chromatographic profiles shown in fig. 1, 2 and 5.

Carotenoid pigments are a group of natural pigments showing yellow, orange, red, etc., and a very wide variety of carotenoids have been isolated from animals and plants to date. Carotenoid as a compound having generally 8 isoprene units bonded₄₀H₅₆One of the terpenes of the basic skeleton of (A) is known and classified as tetraterpenes. Carotenoids exhibit greatly different absorption spectra between 400 to 500nm from covalent double bonds in their molecular structures, and thus, appear in yellow to orange to red colors.

As a result of further intensive studies, the present inventors succeeded in identifying compounds corresponding to these peaks, and obtained a surprising finding that the compounds corresponding to these peaks have a basic skeleton of novel xanthophylls.

Accordingly, in a further embodiment of the present invention, there is provided a pianist plant comprising at least 1 or more carotenoid pigments in its petals, wherein said carotenoid pigment is neolutein or a derivative thereof.

Neolutein is an intermediate in the biosynthesis of the plant hormone abscisic acid, and is synthesized from cordierite by neolutein synthase, and has the following chemical structure.

[ CHEM 1]

While both neolutein and cordierite are xanthophylls that are yellow pigments of carotenoid origin, it is highly unexpected that a cordierite derivative is present in an existing Dryobalanops plant (Opale Citrine) as compared to a new xanthophyll derivative present only in the Dryobalanops plant (09M111-1) of the present invention, as shown in the examples described below.

Examples of the novel xanthophyll derivative include fatty acid esters of novel xanthophyll. Examples of the fatty acid forming the fatty acid ester include, but are not limited to, butyric acid, valeric acid, caproic acid, enanthic acid, caprylic acid, pelargonic acid, capric acid, lauric acid, myristic acid, pentadecanoic acid, palmitic acid, palmitoleic acid, pearlitic acid, stearic acid, oleic acid, isooleic acid, linoleic acid, linolenic acid, eleostearic acid, arachidic acid, midic acid, arachidonic acid, behenic acid, lignoceric acid, nervonic acid, cerotic acid, montanic acid, and melissic acid. The neoxanthophyll derivative is preferably a neoxanthophyll diester in which a fatty acid is bonded to each of the hydroxyl groups at the 3-position and 3' -position of neoxanthophyll, and particularly preferably neoxanthophyll dimyristate, neoxanthophyll 3-O-myristic-3 ' -O-palmitic acid, neoxanthophyll 3-O-palmitic acid-3 ' -O-myristic acid, and neoxanthophyll dipalmitate.

In addition, the present invention contemplates that the petals of the marshmallow plant contain neolutein or a derivative thereof as a carotenoid pigment, and the petals of the plant may contain neolutein synthase as an enzyme involved in biosynthesis thereof. The neolutein synthase is an enzyme that introduces allenyl group into cordierite to convert it into neolutein, and produces neolutein using cordierite as a substrate. Thus, in a further embodiment of the invention, there is provided a plant of the genus piper having petals comprising neolutein synthase.

The Pistacia plant of the present invention may have a color pattern of-10 to 70 a and 0 to 80 b in the CIE L A B color system. In this case, the flower color is preferably a value a of 0 to 30 and a value b of 0 to 5. Most preferably, the color has a color of-5 to 20a, and a color of 3 to 70 b, or a color of 50 to 70 a, and a color of 5 to 70 b.

The CIE L a b color system is a color system standardized by the international commission on illumination (CIE), and is widely known as CIE1976(L a b) color system as a method of expressing color tones. In the L × a × b color system, the luminance is represented by L, and the chromaticities indicating the hue and chroma are represented by a × and b. a and b each indicate a direction of color, a indicates a direction of red, -a indicates a direction of green, b indicates a direction of yellow, and further-b indicates a direction of blue. The values of L, a, and b can be derived from tristimulus values X, Y, Z according to the following equations.

L*＝116(Y/Y₀)^1/3-16；

a*＝500[(X/X₀)^1/3-(Y/Y₀)^1/3](ii) a And

b*＝200[(Y/Y₀)^1/3-(Z/Z₀)^1/3]

however, X/X₀、Y/Y₀And Z/Z₀Is that>0.008856 in the formula, X₀、Y₀And Z₀Representing the tristimulus value of a standard light source.

And 2 different colors, L₁、a*₁And b₁And L₂、a*₂And b₂The color difference Δ E of (a) can be obtained by the following equation.

ΔE＝√(L*₂-L*₁)²+(a*₂-a*₁)²+(b*₂-b*₁)²

The analysis of the color in the CIE L a b color system can be easily performed by an integrating sphere type spectrophotometer, and a commercially available spectrophotometer (CM-2022, konica minolta) can be used, for example.

The present invention may further contain 0.02mg or more of anthocyanin pigment per 1g of fresh petals of the plant of the genus Pimpinella, and preferably contains 0.05mg or more, most preferably 0.07mg or more of anthocyanin pigment.

Anthocyanin pigments are known to be contained in plants and present red, blue, and purple colors. The aglycone is classified into 3-series of pelargonidin, anthocyanidin and delphinidin according to the number of hydroxyl groups in the B ring, which is the anthocyanin site. It is known that the chromogens are in the aglycon part, pelargonidin is bright red, anthocyanidin is reddish purple, delphinidin is purplish red. The main anthocyanin pigment contained in the muscadine plant of the present invention is anthocyanin.

The Pimenta plant of the present invention is not particularly limited as long as it satisfies the above requirements, but hybrid plants having 09M111-1 and 09M111-1 as parent plants are preferably used. 09M111-1 cultured seedlings were deposited at the national institute of advanced Industrial science and technology (NITE) patent organism depositary center for evaluation of technology (NITE-IPOD) of independent administrative sciences on 9/10/2015 under the Budapest treaty (namely, Japanese national institute of advanced Industrial science and technology, International patent organism depositary) (accession No. 120, 2-5-8 of Total Sickle foot on Kyowa Kagaku K.K.) (deposition No. FERM BP-22298).

The present invention encompasses progeny of the aforementioned driftwood plant. Such progeny preferably have the same desirable characteristics as the aforementioned driftwood plants. The present invention also encompasses vegetative propagules (cuttings, seeds, etc.) of the aforementioned Dryobalanops plants or their progeny, parts (flowers, stems, branches, leaves, roots, etc.), tissues, or cells of the plant body. The present invention also includes a cut flower of the aforementioned Dryobalanops plant or its progeny, or a processed product made from the cut flower.

In embodiment 2 of the present invention, there is provided a method for selecting a parent bauhinia plant having a carotenoid pigment in its petals, which is capable of crossing a bauhinia plant having white to pink to red petals, characterized in that the carotenoid pigment is extracted from the petals of a candidate bauhinia plant, and when a compound (specifically, the above-mentioned neolutein or a derivative thereof) corresponding to peak a, peak C or peak D in the chromatographic spectrum shown in fig. 1, 2 and 5 or a neolutein synthase is present as the carotenoid pigment in an extract solution, the parent bauhinia plant is selected. Confirmation of the novel xanthophyll synthase can be carried out by methods routine to those skilled in the art, for example, identification of the novel xanthophyll synthase gene by PCR method or direct identification of the novel xanthophyll synthase by mass spectrometry.

The structure of the flowers of the marsdenia plant is special, and artificial mating through artificial pollination is technically difficult. Although there are about 130 or more species, there are not more than 10 species used in horticulture. Therefore, although there are wild species having yellow flower colors, a hybrid spathula plant having a conventional variety having yellow flower colors as a mating parent has not been produced so far, and new color expression by the hybridization between a spathula variety having yellow flower colors and a conventional spathula variety having white to pink to red flower colors has not been realized. However, it becomes easy to select as a parent plant a pterocarpus plant having a carotenoid-based flower color which can be hybridized with a pterocarpus plant having white to pink to red petals by the method of the present invention.

In the method for selecting a parent sweethearts according to the present invention, the color tone of petals of candidate sweethearts is measured in CIE L a b color system, and when a value is-10 to 70 and b value is 0 to 80, the parent sweethearts may be selected. In this case, when the value a is 0 to 30 and the value b is 0 to 5, the parent Pimenta plant is preferably excluded. Most preferably, the color tone of petals of candidate sweethearts is measured in the CIE L a b color system, and the candidate sweethearts are selected as parent sweethearts when a is-5 to 20 and b is 3 to 70, or when a is 50 to 70 and b is 5 to 70.

In the method for selecting a parent sweetclover plant of the present invention, when the amount of anthocyanin pigment in the petals of candidate sweetclover plants is measured and the anthocyanin pigment is contained in an amount of 0.02mg or more, preferably 0.05mg or more, and most preferably 0.07mg or more per 1g of fresh petals, the parent sweetclover plant may be selected. The anthocyanin pigment is suitably an anthocyanin.

The parent Pimenta plant is not particularly limited as long as it is obtained by the above-described selection method of the present invention, and suitable examples thereof include hybrid plants having 09M111-1 and 09M111-1 as parent plants.

Furthermore, the present invention also includes a method for producing a plant of the genus Pistacia having carotenoid pigments in its petals, comprising: the method of selecting a parent sweethearts according to the present invention, the step of selecting the 1 st sweethearts parent plant, and the step of crossing the 1 st sweethearts parent plant and the 2 nd sweethearts parent plant.

The 2 nd drift vine parent plant is not particularly limited as long as it can be crossed with the 1 st drift vine parent plant, but is preferably a drift vine plant having white to pink to red petals. For example, there are large round opening "deep red king" (Mandevilla hybrida 'Sunmandecrikin'), large round opening type "white" (Mandevilla amabilis x boliviaensis 'Sunmandeho'), and blooming type "tropical peach red" (Mandevilla hybrida 'Sunmandeomi').

Crossing is performed by natural mating or artificial mating. In the saxifraga plant, the maturation period of the seeds is about 6 months, which is very long compared to that of a normal plant, and therefore, it is important to maintain the parthenocarpic stem without cutting the parthenocarpic stem until the seeds mature to some extent after mating.

The present invention can produce a new sweetgum plant having a new color tone, particularly a color tone ranging from yellow to apricot to orange (e.g., ivory, light yellow, apricot yellow, orange pink, orange red, orange), which has not been produced in the past.

[ examples ] A method for producing a compound

[ example 1. preparation of Pistacia plant ]

In 5 months in 2009, mating was carried out in a greenhouse at the world reproductive & resource center (east-and-river city) of Sandeli flowers (plants) as a mating material, using MR-7 (which died later) of a variety with a red color, which the Sandeli flowers (plants) hold, as a female parent, and using Sunparasol pure white (registered species name: Sunparakoho), which is white in color, as a male parent. Mating seeds were harvested at 11 months of 2009. Sowing in the greenhouse in 11 months in 2009. 100 well-grown seedlings were selected from the germinated seedlings, transplanted into 9cm pots, grown in a greenhouse managed at 16 ℃ at the lowest night, transplanted into 18cm pots in 5 months in 2010, and subjected to cultivation tests in an outdoor field in the center of the world breeding & resources of Sandeli flowers (plants). Flowering plants were selected at 9 months in 2010 using the novelty, growth ability, and heat tolerance of flower color as indicators, and 09M111-1 having apricot yellow flower color (L: 81.8, a: 11.7, and b: 28.3) was selected.

The selfing mating of 09M111-1 was performed in Sandeli flowers (plants) in the global reproduction & resource center greenhouse 10 months in 2010. The selfed seeds were harvested at 5 months in 2011. Sowing in the greenhouse in 11 months in 2011. 100 well-grown seedlings were selected from the germinated seedlings, transplanted to 9cm pots, grown in a greenhouse managed at 16 degrees at the lowest night, transplanted to 18cm pots in 2012 at 5 months, and subjected to cultivation tests in an outdoor field of Sandeli flowers (plants). The individual 09M111-1self-1 with strong yellow taste using flower color as an index was selected from flowering plants at 9 months 2012. Thereafter, mating with 09M111-1 as female parent and 09M111-1self-1 as male parent was carried out in 5 months in 2013 in the Sandeli flower (strain) Global reproduction & resource center greenhouse. Mating seeds were harvested in 2013 at 11 months. Sowing in the greenhouse in 11 months in 2013. 100 well-grown seedlings were selected from the germinated seedlings, transplanted into 9cm pots, grown in a greenhouse managed at 16 degrees at the lowest night, transplanted into 18cm pots in 5 months 2014, and cultivation tests were performed in outdoor fields in the global breeding & resource center of Sandeli flowers (plants). From flowering plants in 9 months in 2014, #4990 with yellow flower colors (L ═ 82.4, a ═ 8.6, b ═ 48.0) was selected, with the novelty, growth ability, and heat tolerance of flower colors as indices.

In the field outside in the center of the world reproduction & resources of Sandeli flowers (plants) in 5 months in 2013, 09M111-1self-1 and a plurality of red lines with high petal anthocyanin content were arranged close to each other, and then let-off pollination was performed. The fruiting seeds with 09M111-1self-1 as female parent were harvested 11 months in 2013. Sowing in the greenhouse in 11 months in 2013. 100 well-grown seedlings were selected from the germinated seedlings, transplanted into 9cm pots, grown in a greenhouse managed at 16 ℃ at the lowest night, transplanted into 18cm pots in 5 months in 2014, and managed in an outdoor field of Sandeli flowers (plants). From flowering plants in 9 months in 2014, #4891 with orange flower color (L ═ 36.2, a ═ 54.0, b ═ 41.4) was selected, with the novelty, growth ability, and heat tolerance of flower color as indices.

[ example 2. analysis of carotenoid pigments ]

As for the cultivar of Pimpinella, the carotenoid pigment in the petals of 4990, 09M111-1 and Opale Citrine was quantified as the content in the cultivar as follows.

Quantitative analysis was performed by a high performance liquid chromatography-separation-personal digital assistant (HPLC-PDA) system capable of utilizing detection by a photodiode array detector (PDA) capable of analyzing yellow pigment well.

The preparation method of the detection solution comprises weighing 5mg of lyophilized petals in a glass bottle, adding 0.5mL of acetone, and performing ultrasonic irradiation with an ultrasonic cleaner (ASONE, AS52GTU) at 25 deg.C for 20 min to extract yellow pigment component. HPLC-PDA detection was carried out by filtering 0.3mL of the supernatant from which the yellow pigment component was extracted with a filter having a pore size of 0.45 μm (NACALALI TESSQUE, Cosmonce filter S (solvent system)).

The HPLC-PDA (high Performance liquid chromatography separation-photodiode array Detector) was constructed by using a Nexera LC-30AD (Shimadzu corporation) as a liquid-feeding unit LC pump, using a prominine SPD-M20A (Shimadzu corporation) as a PDA detector, and using a Develosil C30-UG-32.0X 100mm (Nomura chemical) as a separation column. The liquid transfer of the LC mobile phase was carried out at a flow rate of 0.4 mL/min by using a binary gradient of methanol/water (80/20, vol/vol) for mobile phase A and t-butyl methyl ether/methanol/water (78/20/2, vol/vol/vol) for mobile phase B to change the concentration of mobile phase B from 0% to 100% over 15 minutes. 10 μ L of a sample solution extracted from petals was injected.

The absorbance of the PDA detector was measured in the range of 200-800nm, and the absorption band 440-490nm of the blue color, which is a complementary color to the yellow color, was particularly noted. Quantitative values used were absorbance of each peak obtained from 450nm LC-PDA.

The results are shown in FIGS. 1 to 3.

[ example 3 analysis of Flavonoids ]

Petals of Piper hancei were collected, freeze-dried, and flavonoids were extracted from the petals with 50% acetonitrile (v/v) containing 0.1% trifluoroacetic acid (TFA). 0.2ml of the obtained extract was dried by dividing, and then dissolved in 0.2ml of 6N HCl, followed by treatment at 100 ℃ for 20 minutes to obtain anthocyanins. It was extracted with 0.2ml of 1-pentanol and subjected to HPLC analysis. Using an ODS-A312 column (15 cm. sup. -6 mm; YMC) with an isocratic solvent (AcOH: MeOH: H)₂O, 15: 20: 65, v/v/v), analysis was performed at a flow rate of 1ml/1 minute.

Then, 200. mu.l of the petal extract adjusted as described above was collected and dried, and then dissolved in 0.2ml of 0.1M potassium phosphate buffer containing 6 units of β -glucosidase (Sigma, St. Louis, Mo., USA) and 1 unit of naringinase (Sigma) and treated at 30 ℃ for 16 hours, whereby flavonol was obtained by enzymatic hydrolysis. To this was added 200. mu.l of 90% (v/v) acetonitrile containing 0.1% TFA, and after the reaction was stopped, the mixture was subjected to HPLC analysis. Using a Shim-pack FC-ODS column (15 cm-4.6 mm; Shimadzu corporation),solvent A (H) was used at a flow rate of 0.6 ml/min₂O: TFA, 99.9: 0.1, v/v) and solvent B (H)₂O: acetonitrile: TFA, 9.9: 90: 0.1, v/v). The conditions for gradient analysis are as follows.

0 minute: solvent B20%

0-10 minutes: solvent B70%

10-16 minutes: solvent B70%

16-17 minutes: solvent B20%

17-28 minutes: solvent B20%

Anthocyanin and flavonol were detected with absorbance of 250-400nm using a photodiode array detector (SPD-M20A, Shimadzu Corp.).

The results are shown in the following table.

[ TABLE 1]

[ example 4. color measurement ]

The color values of the crown flat-open part of the variety produced in the present invention and the existing variety were measured by a spectrophotometer (CM2022, minolta) using D65 illumination. The measured values are quantified according to the CIE L a b color system (C.I.E., 1986; Gonnet Hieu, 1992; McLaren, 1976). For each variety, 3 individual flowers were measured, and the average value was used as a representative value. The distribution of the colors is displayed in 2 dimensions using a distribution diagram having chromaticity values a and b as coordinate axes, and the distribution of each color group is confirmed.

The results are shown in FIG. 4.

[ example 5 analysis of yellow pigment ]

The analysis of the characteristic yellow pigment component was performed by comparing 09M111-1 with Opale Citrine.

The structure of the yellow pigment component which imparts petal characteristics in the present invention is analyzed by (i) High Performance Liquid Chromatography (HPLC) -lightAbsorption spectrum of photodiode array detector (PDA), (ii) proton nuclear magnetic resonance spectrum(s) (ii)¹H-NMR) and (iii) High Resolution Mass Spectroscopy (HRMS).

(i) Detection of yellow pigment component using High Performance Liquid Chromatography (HPLC) -photodiode array detector (PDA)

Absorption spectra containing several yellow pigment components were obtained using on-line fractionation and a high performance liquid chromatography-photodiode array detector (HPLC-PDA) capable of obtaining ultraviolet-visible photometric data. The PDA is required to obtain an absorption spectrum in the range of 400 to 500nm which is yellow. Actual PDA data acquisition was performed in the range of 200 to 800 nm.

The sample solution was prepared by weighing 5.0mg of each of the lyophilized petals in a glass bottle, adding 0.5mL of acetone AS an extraction solvent, and then irradiating the resulting mixture with ultrasonic waves using an ultrasonic cleaner (ASONE, AS52GTU) at a set temperature of 25 ℃ for 20 minutes to extract the yellow pigment component. The sample solution was obtained by filtering 0.3mL of the supernatant from which the yellow pigment component was extracted with a filter having a pore size of 0.45 μm (NACALALI TESSQUE, Cosmonce filter S (solvent system)).

As HPLC conditions, a reverse phase system column in which octadecyl (C18) and triacontyl (C30) are chemically modified is preferably used as the separation column, and a mixed solution of tert-butyl methyl ether/methanol/water in which absorption spectrum is not hindered in the range of 300 to 600nm is preferably used as the mobile phase.

The HPLC-PDA was constituted by using a Nexera LC-30AD (Shimadzu corporation) as a liquid-feeding unit LC pump, using a prominine SPD-M20A (Shimadzu corporation) as a PDA detector, and using a Develosil C30-UG-3 (2.0X 100mm) as a separation column (Nomura chemical). The liquid transfer of the LC mobile phase was carried out at a flow rate of 0.4 mL/split by using a binary gradient of methanol/water (80/20vol/vol) for mobile phase A and tert-butyl methyl ether/methanol/water (78/20/2vol/vol/vol) for mobile phase B to change the concentration of mobile phase B from 0% to 100% over 15 minutes. 10. mu.L of petal extract was injected during the analysis.

FIG. 5 shows the chromatograms at 450nm for PDA, 09M111-1 and Opale Citrine.

Comparing the spectra, it was found that the peaks of peak A (retention time 12.0 minutes), peak C (retention time 12.2 minutes), and peak D (retention time 12.4 minutes) in the figure were contained in 09M111-1, but not in Opale Citrine. The absorption spectrum obtained with the PDA detector corresponding to the peak is shown in fig. 6. In the apparent absorption spectrum of all peaks, 3 peaks (419,443,470nm) were observed which are characteristic among carotenoid compounds. These carotenoids and their ester bodies are presumed to have α -carotene, β -carotene, lutein, cordierite, neolutein, etc. in the skeleton from the absorption maximum wavelengths of 3 bands.

(ii) Of yellow pigments¹H-NMR analysis

To perform the chemical structure determination of the carotenoid skeleton of the peak A, C, D characteristically seen in 09M111-1 in FIG. 5, a variety of chemical structure information was given¹H-NMR measurement.

To obtain to¹The amount of the sample required for H-NMR measurement is about 1mg, and about 3g of dried petals is required, and a concentration operation is required. Since carotenoids are likely to undergo isomerization, oxygen addition, and other reactions, it is preferable to store the sample in the presence of an oxygen addition inhibitor such as dibutylhydroxytoluene (BHT) under a nitrogen-sealed light-shielding condition. In addition, in general, of carotenoids¹H-NMR measurement is often carried out in heavy chloroform, but the decomposition of heavy chloroform generates hydrochloric acid, and thus the acid-unstable C-5,6 epoxy carotenoid is sometimes changed to a C-5,8 epoxy group by an acid. Since 09M111-1 and Opale Citrine both discolored in chloroform, C-5, 6-epoxy carotenoids containing epoxidized lutein, cordierite, neolutein, etc. were known.

Details of the preparation of the sample are described in the following (1) extraction of the yellow pigment component, (2) fractionation of the yellow pigment fraction by medium-pressure liquid chromatography, (3) removal of the fatty chain and triacylglycerol by the saponified ester bond, and (4) analysis of the sample dissolved in heavy benzene.

(1) Extraction of yellow pigment component

2.97g of each of the freeze-dried petals was immersed in 100mL of acetone containing 1% dibutylhydroxytoluene (BHT), and stirred at room temperature for 1 hour to extract a yellow pigment component. Thereafter, the mixture was concentrated to about 20mL under reduced pressure.

(2) Fractionation of the yellow pigment fraction by medium pressure liquid chromatography

To concentrate the yellow pigment component from the concentrated sample to be tested, purification was performed using Medium Pressure Liquid Chromatography (MPLC). The MPLC machine was constructed by using Smart Flash EPCLC AI-580S (hill) as the device main body and HI-FLASH ODS-SM (50 μm Size L26X 100mm 35g) as the separation column (hill). The liquid transfer of the LC mobile phase was carried out at a flow rate of 20 ml/min with methanol as the mobile phase A and acetone as the mobile phase B, and with a binary gradient of 40% for 0 to 3 min and a mobile phase B concentration of 100% for 24 min. Recovering the elution fraction 18 to 23 minutes after which the elution fraction having an absorption wavelength of 450nm is observed, and drying the elution fraction.

(3) Removal of fatty chains and triacylglycerols by saponified ester bonds

In the above operations (1) and (2), the fatty chains of triacylglycerols or carotenoid esters contained in the petals give signals that inhibit the analysis of the carotenoid skeleton by NMR. Hydrolysis of the ester is carried out by saponification.

The saponification was carried out by adding 10ml of 5% KOH methanol to the sample dried in (2) and reacting at room temperature for 20 minutes. 10ml of water and methylene chloride were added to each of the mixtures, and the methylene chloride layer was washed with water and saturated physiological saline, and then the solvent was distilled off at 30 ℃ under reduced pressure. Furthermore, to obtain high purity¹H-NMR was purified by HPLC using a sample. HPLC purification was carried out under conditions such that an LC pump (liquid delivery unit) used LC-10A (Shimadzu corporation), an SPD-10A (Shimadzu corporation) used as a UV-vis detector, and CAPCELL PAK C18 UG120 (20X 250mm) (Seikagaku corporation) used as a separation column. The liquid transfer of the LC mobile phase was carried out at a flow rate of 10 mL/min with 90% acetonitrile in water. The resultant extract was subjected to fractionation to a range where absorption was confirmed while observing a UV-vis detector at 450nm, and then dried and cured to give a solid¹H-NMR sample.

(4) Of samples dissolved in heavy benzene¹H-NMR analysis

The yellow pigment component after the saponification treatment was dissolved in heavy benzene and subjected to NMR measurement.¹H-NMR data acquisition application AVANCE III HD400(Bruker). FIG. 7 shows a 400MHz for 09M111-1¹An expanded graph of the H-NMR spectrum in the range of 5.3 to 6.9 ppm. Chemical structure information of the methine (═ CH-) group of the carotenoid covalent double bond is presented. A characteristic signal was observed at 6.06 ppm. This signal shows the partial structure of the 8 allene structure (C ═ C) in the new xanthophyll structure diagram. On the other hand, no signal was observed in the range of 6.0 to 6.1ppm from the NMR spectrum of Opale Citrine (FIG. 8), and signals corresponding to 7(5.91ppm), 4 '(5.53 ppm) and 7' (5.45ppm) in FIG. 8 were observed. From this signal, the possibility of containing epoxidized xanthophylls was estimated.

(iii) Identification of ester chain length by LC-APCI-HRMS

In order to identify carotenoid esters corresponding to the respective peaks, the determined chemical compositions were determined by precision mass spectrometry. Since the extract derived from petals contains compounds other than yellow pigments such as triacylglycerol and a plurality of carotenoid esters, it is an effective method for separating one tube by in-line liquid chromatography in mass spectrometry. Ionization by mass spectrometry was performed by Atmospheric Pressure Chemical Ionization (APCI) reported for mass spectrometry of carotenoids. The mass separation unit is used as an Orbitrap MS of an electric field fourier transform type mass spectrometer for obtaining a mass spectrum having a decomposition energy of 60,000 or more, in order to separate the triacylglycerol by mass or determine the chemical composition with high accuracy.

The sample solution used for the accurate mass measurement was prepared by weighing 50.0mg of each of the freeze-dried petals in a glass bottle, adding 1.0mL of acetone AS an extraction solvent, and then irradiating ultrasonic waves with an ultrasonic cleaner (ASONE, AS52GTU) at a set temperature of 25 ℃ for 30 minutes to extract the yellow pigment component. The sample solution was obtained by filtering 0.3mL of the supernatant from which the yellow pigment component was extracted with a filter having a pore size of 0.45 μm (NACALALI TESSQUE, Cosmonce filter S (solvent system)).

The HPLC-APCI-HRMS was a liquid-delivery unit LC pump using Nexera LC-30AD (Shimadzu corporation) and a separation column using Develosil C30-UG-3 (2.0X 100mm) (Nomura chemical). The liquid transfer of the LC mobile phase was carried out at a flow rate of 0.4 mL/split by using a binary gradient of methanol/water (80/20vol/vol) for mobile phase A and tert-butyl methyl ether/methanol/water (78/20/2vol/vol/vol) for mobile phase B to change the concentration of mobile phase B from 0% to 100% over 15 minutes. 10. mu.L of petal extract was injected during the analysis. The mass spectrometer section used an Orbitrap Elite MS (ThermoFisher scientific) equipped with an APCI ion source. The mass spectrometry is performed in a positive ion measurement mode, with a decomposition energy of 60,000 set, and in a range of m/z 150 to 2000.

Identification of carotenoid compounds from mass spectrometry data was performed by mass extraction ion chromatography using an ion mass corresponding to the theoretical value of the precise mass of the proton-donating ion from (i) an absorption spectrum by HPLC-PDA and (ii) a mass spectrum by HPLC¹The chemical composition of the carotenoid skeleton which is estimated by the analysis of H-NMR is determined by modifying fatty acids such as lauric acid, myristic acid, palmitic acid, palmitoleic acid, and stearic acid. FIG. 9 shows 09M111-1, and FIG. 10 shows that fatty acids of the novel lutein diester and the same mass of the cordierite diester of Opale Citrine are equivalent to those of myristic acid and palmitic acid, and the ion chromatogram is extracted with high decomposition energy of (a) M/z 1021.81-1021.83 (b) M/z 1049.84-1049.86 (c) M/z 1077.87-1077.89. Neoxanthophyll dimyristate, neoxanthophyll myristate palmitate, neoxanthophyll dipalmitate, cordierite myristate, cordierite palmitate, and cordierite palmitate were detected in the chromatogram of 09M111-1 in FIG. 9. On the one hand, only diadermatan dimyristate, diadermatan myristate palmitate, and diadermatan dipalmitate were detected in the Opale Citrine high-resolution mass extraction ion chromatogram of fig. 10.

Fig. 11 (reference data): high resolution mass extraction ion chromatography of Opale Citrine, (a) m/z 1005.82-1005.83, (b) m/z 1033.85-1033.87, (c) m/z 1061.88-1061.90. The peak detected corresponds to the epoxidized lutein diester.

As a result: by (i) absorption spectroscopy using High Performance Liquid Chromatography (HPLC) -photodiode array detector (PDA), (ii) proton nuclear magnetic resonance spectroscopy (NMR)¹H-NMR)、(iii) Structural analysis and identification of high resolution energy mass spectrometry spectrum (HRMS), peak A is constitutional formula C₆₈H₁₀₈O₆New xanthophylls of dimyristolic acid, peak C is a constitutional formula C₇₀H₁₁₂O₆3-O-myristic-3 '-O-palmitic acid neolutein or 3-O-palmitic-3' -O-myristic acid neolutein, and further, peak D is a composition formula C₇₂H₁₁₆O₆The dipalmitoic acid neolutein.

The present specification also includes the following:

1. a Pistacia plant comprising at least 1 or more carotenoid pigments in its petals, wherein said carotenoid pigment is neolutein or a derivative thereof.

2. The Pterocarpus plant according to embodiment 1, wherein the carotenoid pigment is neolutein dimyristate, neolutein 3-O-myristate-3 '-O-palmitate, neolutein 3-O-palmitate-3' -O-myristate, neolutein dipalmitate or a combination thereof.

3. A Pistacia plant comprising at least 1 or more carotenoid pigments in its petals, wherein said carotenoid pigment is a compound corresponding to peak A, peak C or peak D in the chromatographic profiles shown in FIGS. 1, 2 and 5.

4. The petal of the plant of the genus Pimpinella contains neolutein synthase.

5. The Pimenta plant according to any one of embodiments 1 to 4, wherein the Pimenta plant has a color pattern of-10 to 70 a and 0 to 80 b in the CIE L A B color system.

6. The Pimenta plant according to embodiment 5, wherein the plants have a flower color other than a flower color having an a value of 0 to 30 and a b value of 0 to 5 in the CIE L a b color system.

7. The Pimenta plant according to any one of embodiments 1 to 4, wherein the color system of CIE L A B has a color of-5 to 20a, 3 to 70 b, 50 to 70 a, and 5 to 70 b.

8. The Dryobalanops plant according to any one of embodiments 1 to 7, wherein the anthocyanin pigment is contained in an amount of 0.02mg or more per 1g of fresh petals.

9. The muscadine plant of embodiment 8, wherein the anthocyanin pigment is anthocyanidin.

10. The Pimpinella plant is 09M111-1 (preservation number: FERM BP-22298).

11. A progeny of the Pimenta plant of any one of embodiments 1-10.

12. A vegetative propagation material, a part of a plant, a tissue or a cell of a plant of the genus Pimenta according to any one of embodiments 1 to 10.

13. The cut flower of the Pistacia plant according to any one of embodiments 1 to 10, or a processed product made of the cut flower.

14. A method for selecting a parent Pimpinella plant having a carotenoid pigment in its petals, which is capable of crossing Pimpinella plants having white to pink to red (colors having an a value of 0 to 60 and a b value of 0 to 40 in the CIE L A B color system),

when neolutein or a derivative thereof is present as a carotenoid pigment in petals of candidate bauhinia plants, the candidate bauhinia plants are selected as parent bauhinia plants.

15. The method according to embodiment 14, wherein the carotenoid pigment is neolutein dimyristate, neolutein 3-O-myristate-3 '-O-palmitate, neolutein 3-O-palmitate-3' -O-myristate, neolutein dipalmitate, or a combination thereof.

16. A method for selecting a parent Pimpinella plant having a carotenoid pigment in its petals, which is capable of crossing Pimpinella plants having white to pink to red (colors having an a value of 0 to 60 and a b value of 0 to 40 in the CIE L A B color system),

when a compound corresponding to peak a, peak C or peak D in the chromatogram shown in fig. 1, 2 and 5 is present as a carotenoid pigment in petals of candidate spatholobus, the candidate spatholobus is selected as a parent spatholobus.

17. A method for selecting a parent bauhinia plant capable of crossing with bauhinia plants having white to pink to red petals (colors having an a value of 0 to 60 and a b value of 0 to 40 in the CIE L A B color system),

when new lutein synthase is present in petals of candidate sweethearts, the parent sweethearts is selected.

18. The method according to any one of embodiments 14 to 17, wherein the color tone of petals of the candidate muscadine plant is measured in the CIE L a b color system, and the parent muscadine plant is selected when a value is-10 to 70 and b value is 0 to 80.

19. The method of embodiment 18, wherein the value of a is 0 to 30 and the value of b is 0 to 5, excluding selection from the parent sweetcane plant.

20. The method of any one of embodiments 14 to 17, wherein the color tone of the petals of the candidate muscadine plant is measured in the CIE L a b color system, and the parent muscadine plant is selected when the a value is-5 to 20 and the b value is 3 to 70, or when the a value is 50 to 70 and the b value is 5 to 70.

21. The method according to any one of embodiments 14 to 20, wherein the amount of the anthocyanin pigment in the petals of the candidate bauhinia plant is measured, and when the anthocyanin pigment is contained in an amount of 0.02mg or more per 1g of fresh petals, the candidate bauhinia plant is selected as a parent bauhinia plant.

22. The method of embodiment 21, wherein the anthocyanin pigment is anthocyanidin.

23. The method according to any one of embodiments 14 to 22, wherein 09M111-1 (accession number: FERM BP-22298) is selected as a parent Botrychium plant.

24. A method for producing an aspidistra plant having petals for flower colors having-10 to 70 a-values and 0 to 80 b-values in the CIE L a-b color system, comprising:

a step of selecting a parent plant of the genus Pimpinella according to the method described in any one of embodiments 14 to 23, and

a step of crossing the selected 1 st and 2 nd drift vine parent plants.

25. The method of embodiment 24, wherein said 2 nd parent plant of the genus amygdalus has petals that are white to pink to red (flowers and colors with a value of 0 to 60 a, and b value of 0 to 40 b in the CIE L a b color system).