绿藻的bestrophin碳酸氢盐转运蛋白
阅读说明:本技术 绿藻的bestrophin碳酸氢盐转运蛋白 (Benzophin bicarbonate transporter from green algae ) 是由 A·慕克吉 詹姆斯·V·莫罗尼 C·S·刘 L·C·M·麦金德 于 2019-07-12 设计创作,主要内容包括:本公开的方面涉及由于增加植物细胞内碳酸氢盐跨过膜的能力而具有增加的碳利用效率的基因修饰的植物和/或藻类。本公开的其他方面涉及制造这种植物和/或藻类以及栽培这些基因修饰的植物以增加碳利用效率和/或生长这些基因修饰的海藻以增加碳利用效率的方法。(Aspects of the present disclosure relate to genetically modified plants and/or algae having increased carbon utilization efficiency due to increased ability of bicarbonate to cross membranes within plant cells. Other aspects of the disclosure relate to methods of making such plants and/or algae and cultivating these genetically modified plants to increase carbon utilization efficiency and/or growing these genetically modified seaweeds to increase carbon utilization efficiency.)
相关申请的交叉引用
本申请要求于2018年11月19日提交的美国临时申请号62/769,214和于2018年7月13日提交的美国临时申请号62/697,840的权益,其在此通过引用以它们的整体并入。
作为ASCII文本文件的序列表的提交
ASCII文本文件上的以下提交的内容以其整体通过引用并入本文:序列表的计算机可读的形式(CRF)(文件名:794542000140SEQLIST.TXT,记录的日期:2019年6月28日,大小:461KB)。
技术领域
本公开涉及基因修饰的植物。具体地,本公开涉及含有优选地提供增加的碳利用效率的绿藻的bestrophin碳酸氢盐转运蛋白的基因修饰的植物。
背景技术
绿藻和其他光合水生生物体在自然环境中通常暴露于低且波动的CO2条件。有多种因素可以降低这些生物体的CO2可用率,其包括在水中气体的缓慢扩散,两种无机碳(Ci)形式二氧化碳(CO2)和碳酸氢根(HCO3 -)的缓慢相互转化以及pH改变。由于易变的自然环境,大多数水生光合生物体已经进化出在有限的CO2条件下可诱导的二氧化碳浓缩机制(CCM)。CCM允许水生光合生物体比如绿藻有效地浓缩Ci,用于通过Rubisco的固定(Giordano等,Ann Rev Plant Bio 56:99-131,2005)。绿藻莱茵衣藻(Chlamydomonas reinhardtii)的当前CCM模型(Jungnick等,Photosynth Res 121:159-173,2014;Wang和Spalding,PlantPhysiol 166:2040-2050,2014)包括质膜和叶绿体被膜上的碳酸氢盐转运蛋白,其作为CCM的关键成分,允许Ci、尤其是HCO3 -移动通过膜。相互转化CO2和HCO3-的碳酸酐酶是CCM模型的另一重要成分(Mitra等,Can J Bot 83:780-795,2005;Moroney等,Photosynth Res 109:133-149,2011)。
在莱茵衣藻(C.reinhardtii)中,称为蛋白核的区室定位于叶绿体的基底处。蛋白核是在有限的CO2条件下隔绝Rubisco的位置(Kuchitsu等,Plant Cell Phys 29:1269-1278,1988;Rawat等,Planta 198:263-270,1996;Borkhsenious等,Plant Physiol 116:1585-1591,1998)。类囊体小管和微型小管的广泛的网络与蛋白核相关(Engel等,Elife13:04889,2015),推测提供了用于HCO3 -进入蛋白核的途径。在这些小管中发现了类囊体碳酸酐酶CAH3并且假设CAH3将HCO3 -转化为CO2,用于腔内的固定(Moroney和Ynalvez,Eukaryotic Cell 6:1251-1259,2007)。
在高CO2条件(5%v/v)下生长的莱茵衣藻细胞展示出对Ci的低亲和力。当将适应高CO2的细胞暴露于较低CO2条件(例如,环境的0.04%至低于<0.01%v/v),已经报道了对高亲和力Ci转运蛋白的诱导。同时,在细胞中CO2将容易扩散跨过膜(Gutknecht等,J.Gen.Physiol 69:779-794,1977),因为许多研究已经建立了对主动转运系统的需要,以促进Ci(特别地HCO3 -)移动至在低CO2条件下生长的细胞中可发生通过Rubisco固定的位置(Moroney等,Plant Physiol 83:460-463,1987;Sultemeyer等,Planta 176:256-260,1988;Badger等,Physiologia Plant 90:529-536,1994;Ohnishi等,Plant Cell 22:3105-3117,2010)。另外,分子和生理学研究也已经确认在细胞的质膜和叶绿体被膜上多种形式的Ci转运蛋白的发生(Amoroso等,Plant Physiol 116:193-201,1998;Duanmu等,PNAS106:5990-5995,2009;Atkinson等,Plant Biotechnol J 5:12497,2015;Gao等,Plant 82:1-11,2015;Yamano等,PNAS 112:7315-7320,2015)。
在海洋环境中生存的蓝细菌中,质膜处HCO3-转运通常偶联至海水的高外部Na+离子浓度。在发现莱茵衣藻的淡水环境中,转运被认为是H+-偶联的,因为Na+浓度是相对低的(Morth等,Nat Rev Mol Cell Biol 12:60-70,2011;Taylor等,Trends Plant Sci 17:675-684,2012)。从而,用莱茵衣藻和团藻(Volvox carteri)的基因组研究已经揭示,存在用于硫酸盐和磷酸盐的H+和Na+偶联的转运蛋白二者(Pootakham等,Plant Physiol 153:1653-1668,2010)。还不清楚,是否该类型的离子偶联对碳酸氢盐摄取也是重要的。
迄今为止,已经表征了莱茵衣藻中的两种高亲和力碳酸氢盐转运蛋白质和一种低亲和力碳酸氢盐转运蛋白质并且已知在低CO2条件下有功能。第一高亲和力转运蛋白,高光活化蛋白3(HLA3),为多药耐受性蛋白家族的ATP-结合盒(ABC)-型转运蛋白,并且定位于质膜(Im和Grossman,2002)。Hla3转录物由高光和低CO2条件二者诱导并且被由Cia5基因编码的CCM'主调节物'控制。Duanmu等(2009)显示,在HLA3 RNAi敲低突变体中Ci亲和力和Ci摄取的显著降低,支持该蛋白质在HCO3 -转运中的作用。第二高亲和力转运蛋白,低碳可诱导蛋白质1(LCI1),为相对小的蛋白质。LCI1在低CO2条件下生长的细胞中被强烈上调,并且其已经定位于质膜(Ohnishi等,Plant Cell 22:3105-3117,2010)。另外,Lcr1(缺乏MYB-转录因子的衣藻菌株)背景中LCI1蛋白质的过表达导致增加的Ci摄取。因此,HLA3和LCI1都被认为是位于质膜上的Ci转运蛋白。
第三转运蛋白,NAR1.2(也称为LCIA),为甲酸盐/亚硝酸盐转运蛋白家族的叶绿体被膜蛋白质。尽管NAR1.2蛋白质对碳酸氢盐具有较低亲和力(如以落入mM范围中的K1/2值中揭示的),但在非洲爪蟾(Xenopus Laevis)卵母细胞中表达NAR1.2导致增加的HCO3 -摄取(Mariscal等,Protist157:421-433,2006;Atkinson等,Plant Biotechnol J 5:12497,2015)。已经显示NAR1.2被定位于叶绿体被膜,并且被认为参与Ci摄取,但是对此的分子机制仍然不清楚(Yamano等,PNAS 112:7315-7320,2015)。实验结果指示NAR1.2和HLA3蛋白质在CCM内具有合作的作用(Yamano等,PNAS 112:7315-7320,2015)。在非常低CO2条件下,已经显示NAR1.2在与低-CO2可诱导蛋白质B(LCIB)的缀合中起作用。这些结果提示这样的模型,其中LCIB参与CO2摄取和对从蛋白核泄漏的CO2的回收,而NAR1.2参与HCO3 -摄取和转运途径(Wang和Spalding,Plant Physiol 166:2040-2050,2014)。
除了蛋白质可能参与以上所述的CCM,已经提示多种其他蛋白质的作用。例如,两种可溶性蛋白质LCIB和LCIC形成复合物,已经观察到当细胞适应非常低CO2时,该复合物与蛋白核紧密缔合(Yamano等,Plant Cell Physiol 51:1453-1468,2010),但是还不确认该复合物可能的作用(Jin等,PNAS 113:14716-14721,2016)。另一示例通过CCP1和CCP2提供,其为其他推定的Ci转运蛋白,已经显示被定位于线粒体(Atkinson等,Plant Biotechnol J5:12497,2015)。该定位提示线粒体在CCM功能中是重要的,但是还不清楚该机制可能是什么,或线粒体可起什么作用。
Bestrophin为膜蛋白质的家族,其展示出Cl-通道活性并且也用作电压门控的Ca2+通道的调节物。已经发现人和小鼠bestrophin对HCO3 -具有高透过性和电导性(Qu和Hartzell,Am J Physiol Cell Physiol 294:C1371-C1377,2008)。然而,使用蛋白质比对和系统进化树分析,与哺乳动物中它们的相对物相比,已经显示光养的bestrophin蛋白质中的氨基酸显示高多样性。例如,动物bestrophin中形成Ca2+传感器的残基在拟南芥属中的bestrophin-样蛋白质(AtBest1和AtBest2)中不保守,提示叶绿体中AtBest通道激活不需要Ca2+。所以在进化期间,光养生物中的bestrophin蛋白质已获取与哺乳动物中那些不同的电生理学特性(Duan等Journal of Integrative Plant Biology 58:848-858,2016)。在莱茵衣藻中,已经提示LCI11和Cre16.g662600作为推定的bestrophin,并且建议Cre16.g663400为bestrophin-样蛋白质(Mackinder等,Cell 171:133-147,2017),但是这些没有被进一步表征。
现在的数据仅明确地支持对于莱茵衣藻中的无机碳(Ci)流入的五种蛋白质的需要,五种蛋白质包括HLA3、LCI1、LCIA、CAH3和LCIB(Mackinder,New Phytologist 217:54-61,2018)。如以上所述的,这些蛋白质定位于质膜和叶绿体被膜。莱茵衣藻CCM的现在的模型提示,需要叶绿体类囊体膜中至少一种另外转运蛋白或通道以维持HCO3 -从基质至腔中CAH3的流量(Mackinder New Phytologist 217:54-61,2018)。而且,鉴定功能性碳酸氢盐转运蛋白质仍然是重要的目标。现在的研究已经鉴定了许多可能的碳酸氢盐转运蛋白质,但是仅这些中的少数具有有希望的实验结果,并且极少机制数据可用。
发明内容
为了满足这些需要,本公开涉及充当碳酸氢盐转运蛋白质的绿藻的bestrophin多肽。本公开的某些方面涉及含有增加或提供碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力的一个或多个基因改变的基因改变的植物或其部分。在一些方面中,本公开涉及含有增加或提供碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力的一个或多个基因改变的基因改变的植物或其部分。在一些实施方式中,碳酸氢盐膜跨过能力的获得是至少一种绿藻的bestrophin多肽的表达的结果。在一些实施方式中,绿藻的bestrophin多肽选自SEQ ID NO:1、SEQ ID NO:2或SEQ ID NO:3的组。在一些实施方式中,碳酸氢盐跨过膜的能力的增加或提供是选自与SEQ ID NO:1具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性或至少95%序列同一性的第一多肽,与SEQ ID NO:2具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性或至少95%序列同一性的第二多肽,与SEQ ID NO:3具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性或至少95%序列同一性的第三多肽或其任何组合的组中的多肽的表达的结果。在一些实施方式中,碳酸氢盐跨过膜的能力的增加或提供是选自与SEQ ID NO:1具有至少95%序列同一性的第一多肽、与SEQ ID NO:2具有至少95%序列同一性的第二多肽、与SEQ ID NO:3具有至少95%序列同一性的第三多肽或其任何组合的组中的多肽的表达的结果。在一些实施方式中,碳酸氢盐跨过膜的能力的增加或提供是选自SEQ ID NO:4、SEQ ID NO:5、SEQ ID NO:6、SEQ ID NO:7、SEQ ID NO:8、SEQ ID NO:9、SEQ ID NO:10、SEQ ID NO:11、SEQ ID NO:12、SEQ ID NO:13、SEQ ID NO:14、SEQ ID NO:15、SEQ ID NO:16、SEQ ID NO:17、SEQ ID NO:18、SEQ IDNO:19、SEQ ID NO:20、SEQ ID NO:21、SEQ ID NO:22、SEQ ID NO:23、SEQ ID NO:24、SEQ IDNO:25、SEQ ID NO:26、SEQ ID NO:27、SEQ ID NO:28、SEQ ID NO:29、SEQ ID NO:30、SEQ IDNO:31、SEQ ID NO:32、SEQ ID NO:33、SEQ ID NO:34、SEQ ID NO:35、SEQ ID NO:36、SEQ IDNO:37、SEQ ID NO:38、SEQ ID NO:39、SEQ ID NO:40、SEQ ID NO:41、SEQ ID NO:42、SEQ IDNO:43、SEQ ID NO:44、SEQ ID NO:45、SEQ ID NO:46、SEQ ID NO:47、SEQ ID NO:48、SEQ IDNO:49、SEQ ID NO:50、SEQ ID NO:51、SEQ ID NO:52、SEQ ID NO:53、SEQ ID NO:54、SEQ IDNO:55、SEQ ID NO:56、SEQ ID NO:57、SEQ ID NO:58、SEQ ID NO:59、SEQ ID NO:60、SEQ IDNO:61、SEQ ID NO:62、SEQ ID NO:63、SEQ ID NO:64、SEQ ID NO:65、SEQ ID NO:66、SEQ IDNO:67、SEQ ID NO:68、SEQ ID NO:69、SEQ ID NO:70、SEQ ID NO:71、SEQ ID NO:72、SEQ IDNO:73、SEQ ID NO:74、SEQ ID NO:75、SEQ ID NO:76、SEQ ID NO:77、SEQ ID NO:78、SEQ IDNO:79、SEQ ID NO:80、SEQ ID NO:81、SEQ ID NO:82、SEQ ID NO:83、SEQ ID NO:84、SEQ IDNO:85、SEQ ID NO:86、SEQ ID NO:87、SEQ ID NO:88、SEQ ID NO:89、SEQ ID NO:90、SEQ IDNO:91、SEQ ID NO:92、SEQ ID NO:93、SEQ ID NO:94、SEQ ID NO:95、SEQ ID NO:96、SEQ IDNO:97、SEQ ID NO:98、SEQ ID NO:99、SEQ ID NO:100、SEQ ID NO:101、SEQ ID NO:102、SEQID NO:103、SEQ ID NO:104、SEQ ID NO:105、SEQ ID NO:106、SEQ ID NO:107、SEQ ID NO:108、SEQ ID NO:109、SEQ ID NO:110或SEQ ID NO:111的组,优选地选自SEQ ID NO:10、SEQID NO:11、SEQ ID NO:15、SEQ ID NO:27、SEQ ID NO:28、SEQ ID NO:29、SEQ ID NO:42、SEQID NO:62或SEQ ID NO:63的组中的多肽的表达的结果。在一些实施方式中,多肽定位于植物细胞内至少一种叶绿体的叶绿体被膜或类囊体膜。在一些实施方式中,植物细胞是叶片叶肉细胞。在一些实施方式中,多肽在植物的至少70%的叶片叶肉细胞中表达。
在一些方面中,本公开涉及具有增加的碳利用效率的植物或其部分,其含有具有植物或其部分中绿藻的bestrophin多肽的至少一种编码序列的至少一种修饰的核酸序列,其中bestrophin多肽在植物或其部分中表达,并且其中当在环境二氧化碳条件下栽培植物时,产率、生长速率或生物量(biomass)大于来自不过表达bestrophin多肽的相应的野生型(WT)植物或其相应的WT部分的产率、生长速率或生物量或者产率、生长速率或生物量基本上类似于来自在环境二氧化碳条件下栽培的不过表达bestrophin多肽的相应的野生型(WT)植物或其相应的WT部分的产率、生长速率或生物量。在一些实施方式中,bestrophin多肽定位于植物细胞的至少一种叶绿体的叶绿体被膜或叶绿体类囊体膜。在一些实施方式中,植物细胞是叶片叶肉细胞。在一些实施方式中,多肽在植物的至少70%的叶片叶肉细胞中表达。
在一些方面中,本公开涉及具有增加的水利用效率的植物或其部分,其含有具有植物或其部分中绿藻的bestrophin多肽的至少一种编码序列的至少一种修饰的核酸序列,其中bestrophin多肽在植物或其部分中表达,和其中当在环境二氧化碳条件下栽培植物时,产率、生长速率或生物量大于来自不过表达bestrophin多肽的相应的野生型(WT)植物或其相应的WT部分的产率、生长速率或生物量或者产率、生长速率或生物量基本上类似于来自在环境二氧化碳条件下栽培的不过表达bestrophin多肽的相应的野生型(WT)植物或其相应的WT部分的产率、生长速率或生物量。在一些实施方式中,bestrophin多肽定位于植物细胞的至少一种叶绿体的叶绿体被膜或叶绿体类囊体膜。在一些实施方式中,植物细胞是叶片叶肉细胞。在一些实施方式中,多肽在植物的至少70%的叶片叶肉细胞中表达。
在一些方面中,本公开涉及具有增加的氮利用效率的植物或其部分,其含有具有植物或其部分中绿藻的bestrophin多肽的至少一种编码序列的至少一种修饰的核酸序列,其中bestrophin多肽在植物或其部分中表达,和其中当在环境二氧化碳条件下栽培植物时,产率、生长速率或生物量大于来自不过表达bestrophin多肽的相应的野生型(WT)植物或其相应的WT部分的产率、生长速率或生物量或者产率、生长速率或生物量基本上类似于来自在环境二氧化碳条件下栽培的不过表达bestrophin多肽的相应的野生型(WT)植物或其相应的WT部分的产率、生长速率或生物量。在一些实施方式中,bestrophin多肽定位于植物细胞的至少一种叶绿体的叶绿体被膜或叶绿体类囊体膜。在一些实施方式中,植物细胞是叶片叶肉细胞。在一些实施方式中,多肽在植物的至少70%的叶片叶肉细胞中表达。
在一些方面中,本公开涉及具有减少的光抑制的植物或其部分,其含有具有植物或其部分中绿藻的bestrophin多肽的至少一种编码序列的至少一种修饰的核酸序列,其中bestrophin多肽在植物或其部分中表达,和其中当在环境二氧化碳条件下栽培植物时,产率、生长速率或生物量大于来自不过表达bestrophin多肽的相应的野生型(WT)植物或其相应的WT部分的产率、生长速率或生物量或者产率、生长速率或生物量基本上类似于来自在周围环境二氧化碳条件下栽培的不过表达bestrophin多肽的相应的野生型(WT)植物或其相应的WT部分的产率、生长速率或生物量。在一些实施方式中,bestrophin多肽定位于植物细胞的至少一种叶绿体的叶绿体被膜或叶绿体类囊体膜。在一些实施方式中,植物细胞是叶片叶肉细胞。在一些实施方式中,多肽在植物的至少70%的叶片叶肉细胞中表达。
在任何以上实施方式的一些实施方式中,修饰的核酸序列稳定地整合入植物的细胞核基因组。在任何以上实施方式的一些实施方式中,至少一种修饰的核酸序列另外含有可操作地连接至绿藻的bestrophin多肽的至少一种编码序列的、编码信号肽序列或靶向序列的第二核酸序列,其中信号肽序列或靶向序列的表达导致bestrophin多肽定位于植物细胞的至少一种叶绿体的叶绿体被膜或叶绿体类囊体膜。
在任何以上实施方式的一些实施方式中,增加的碳利用效率、增加的水利用效率、增加的氮利用效率或减少的光抑制是选自与SEQ ID NO:1具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性或至少95%序列同一性的第一多肽,与SEQ ID NO:2具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性或至少95%序列同一性的第二多肽,与SEQ ID NO:3具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性或至少95%序列同一性的第三多肽或其任何组合的组中的多肽的表达的结果。在任何以上实施方式的一些实施方式中,增加的碳利用效率、增加的水利用效率、增加的氮利用效率或减少的光抑制是选自与SEQ IDNO:1具有至少95%序列同一性的第一多肽,与SEQ ID NO:2具有至少95%序列同一性的第二多肽,与SEQ ID NO:3具有至少95%序列同一性的第三多肽或其任何组合的组中的多肽的表达的结果。在任何以上实施方式的一些实施方式中,碳酸氢盐跨过膜的能力的增加或提供是选自SEQ ID NO:4、SEQ ID NO:5、SEQ ID NO:6、SEQ ID NO:7、SEQ ID NO:8、SEQ IDNO:9、SEQ ID NO:10、SEQ ID NO:11、SEQ ID NO:12、SEQ ID NO:13、SEQ ID NO:14、SEQ IDNO:15、SEQ ID NO:16、SEQ ID NO:17、SEQ ID NO:18、SEQ ID NO:19、SEQ ID NO:20、SEQ IDNO:21、SEQ ID NO:22、SEQ ID NO:23、SEQ ID NO:24、SEQ ID NO:25、SEQ ID NO:26、SEQ IDNO:27、SEQ ID NO:28、SEQ ID NO:29、SEQ ID NO:30、SEQ ID NO:31、SEQ ID NO:32、SEQ IDNO:33、SEQ ID NO:34、SEQ ID NO:35、SEQ ID NO:36、SEQ ID NO:37、SEQ ID NO:38、SEQ IDNO:39、SEQ ID NO:40、SEQ ID NO:41、SEQ ID NO:42、SEQ ID NO:43、SEQ ID NO:44、SEQ IDNO:45、SEQ ID NO:46、SEQ ID NO:47、SEQ ID NO:48、SEQ ID NO:49、SEQ ID NO:50、SEQ IDNO:51、SEQ ID NO:52、SEQ ID NO:53、SEQ ID NO:54、SEQ ID NO:55、SEQ ID NO:56、SEQ IDNO:57、SEQ ID NO:58、SEQ ID NO:59、SEQ ID NO:60、SEQ ID NO:61、SEQ ID NO:62、SEQ IDNO:63、SEQ ID NO:64、SEQ ID NO:65、SEQ ID NO:66、SEQ ID NO:67、SEQ ID NO:68、SEQ IDNO:69、SEQ ID NO:70、SEQ ID NO:71、SEQ ID NO:72、SEQ ID NO:73、SEQ ID NO:74、SEQ IDNO:75、SEQ ID NO:76、SEQ ID NO:77、SEQ ID NO:78、SEQ ID NO:79、SEQ ID NO:80、SEQ IDNO:81、SEQ ID NO:82、SEQ ID NO:83、SEQ ID NO:84、SEQ ID NO:85、SEQ ID NO:86、SEQ IDNO:87、SEQ ID NO:88、SEQ ID NO:89、SEQ ID NO:90、SEQ ID NO:91、SEQ ID NO:92、SEQ IDNO:93、SEQ ID NO:94、SEQ ID NO:95、SEQ ID NO:96、SEQ ID NO:97、SEQ ID NO:98、SEQ IDNO:99、SEQ ID NO:100、SEQ ID NO:101、SEQ ID NO:102、SEQ ID NO:103、SEQ ID NO:104、SEQ ID NO:105、SEQ ID NO:106、SEQ ID NO:107、SEQ ID NO:108、SEQ ID NO:109、SEQ IDNO:110或SEQ ID NO:111的组,优选地选自SEQ ID NO:10、SEQ ID NO:11、SEQ ID NO:15、SEQ ID NO:27、SEQ ID NO:28、SEQ ID NO:29、SEQ ID NO:42、SEQ ID NO:62或SEQ ID NO:63的组中的多肽的表达的结果。
在任何以上实施方式的一些实施方式中,植物是豇豆(即,黑眼豌豆、豇豆(Vignaunguiculata)),大豆(即,黄豆、大豆(Glycine max)),木薯(即,树薯、木薯(Manihotesculenta)),水稻(即,稻(Oryza sativa)、非洲栽培稻(Oryza glaberrima)、菰属物种(Zizania spp.)),小麦(即,普通小麦、斯佩耳特小麦、硬质小麦、小麦(Triticumaestivum)、斯佩耳特小麦(Triticum spelta)、硬质小麦(Triticum durum)、小麦属物种(Triticum spp.)),大麦(即,栽培大麦(Hordeum vulgare)),黑麦(即,黑麦(Secalecereale)),燕麦(即,燕麦(Avena sativa)),土豆(即,马铃薯(Solanum tuberosum)),西红柿(即,番茄(Solanum lycopersicum))或另一C3作物植物。在一些实施方式中,植物是烟草(即,普通烟草(Nicotiana tabacum)、Nicotiana edwardsonii、Nicotianaplumbagnifolia、Nicotiana longiflora)或拟南芥属(即,筷子芥,阿拉伯芥、拟南芥(Arabidopsis thaliana))。在任何以上实施方式的一些实施方式中,植物不是玉米(即,玉蜀黍、玉米(Zea mays)),高粱(即,durra、大谷子、milo、高粱(Sorghum bicolor)),甘蔗(即,甘蔗(sugar cane)、甘蔗(Saccharum officinarum)),小米(即,龙爪稷、普通小米、珍珠稷、栗、穇子(Eleusine coracana)、稷(Panicum miliaceum)、珍珠粟(Pennisetumglaucum)、粟(Setaria italica)),柳枝稷(即,高恐慌草、茅草、Panicum virganum)或另一C4作物植物。
在一些实施方式中,任何以上实施方式的植物部分是叶、茎、根、花、种子、果实、细胞或其部分。在一些实施方式中,植物部分是果实。在一些实施方式中,植物部分是籽粒、果仁、豆或块茎。
在一些方面中,本公开涉及任何以上实施方式的花粉粒或胚珠。
在一些方面中,本公开涉及由任何以上实施方式产生的原生质体。
在一些方面中,本公开涉及由任何以上实施方式的原生质体或细胞产生的组织培养物,其中细胞或原生质体由叶、花药、雌蕊、茎、叶柄、根、根尖、果实、种子、花、子叶、下胚轴、胚胎或分生组织细胞的组中的植物部分之一产生。
在一些方面中,本公开涉及含有增加或提供碳酸氢盐跨过膜的能力的一个或多个基因改变的基因改变的种子。在一些实施方式中,种子产生具有碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力的植物。在一些实施方式中,种子产生具有碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力的植物。在一些实施方式中,植物表达至少一种绿藻的bestrophin多肽。在一些实施方式中,绿藻的bestrophin多肽选自SEQ ID NO:1、SEQ ID NO:2、SEQ ID NO:3或其任何组合的组中。在一些实施方式中,植物表达选自与SEQ ID NO:1具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性或至少95%序列同一性的第一多肽,与SEQ ID NO:2具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性或至少95%序列同一性的第二多肽,与SEQ ID NO:3具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性或至少95%序列同一性的第三多肽或其任何组合的组中的至少一种多肽。在一些实施方式中,植物表达选自与SEQ ID NO:1具有至少95%序列同一性的第一多肽、与SEQ ID NO:2具有至少95%序列同一性的第二多肽、与SEQ IDNO:3具有至少95%序列同一性的第三多肽或其任何组合的组中的至少一种多肽。在一些实施方式中,植物表达选自SEQ ID NO:4、SEQ ID NO:5、SEQ ID NO:6、SEQ ID NO:7、SEQ IDNO:8、SEQ ID NO:9、SEQ ID NO:10、SEQ ID NO:11、SEQ ID NO:12、SEQ ID NO:13、SEQ IDNO:14、SEQ ID NO:15、SEQ ID NO:16、SEQ ID NO:17、SEQ ID NO:18、SEQ ID NO:19、SEQ IDNO:20、SEQ ID NO:21、SEQ ID NO:22、SEQ ID NO:23、SEQ ID NO:24、SEQ ID NO:25、SEQ IDNO:26、SEQ ID NO:27、SEQ ID NO:28、SEQ ID NO:29、SEQ ID NO:30、SEQ ID NO:31、SEQ IDNO:32、SEQ ID NO:33、SEQ ID NO:34、SEQ ID NO:35、SEQ ID NO:36、SEQ ID NO:37、SEQ IDNO:38、SEQ ID NO:39、SEQ ID NO:40、SEQ ID NO:41、SEQ ID NO:42、SEQ ID NO:43、SEQ IDNO:44、SEQ ID NO:45、SEQ ID NO:46、SEQ ID NO:47、SEQ ID NO:48、SEQ ID NO:49、SEQ IDNO:50、SEQ ID NO:51、SEQ ID NO:52、SEQ ID NO:53、SEQ ID NO:54、SEQ ID NO:55、SEQ IDNO:56、SEQ ID NO:57、SEQ ID NO:58、SEQ ID NO:59、SEQ ID NO:60、SEQ ID NO:61、SEQ IDNO:62、SEQ ID NO:63、SEQ ID NO:64、SEQ ID NO:65、SEQ ID NO:66、SEQ ID NO:67、SEQ IDNO:68、SEQ ID NO:69、SEQ ID NO:70、SEQ ID NO:71、SEQ ID NO:72、SEQ ID NO:73、SEQ IDNO:74、SEQ ID NO:75、SEQ ID NO:76、SEQ ID NO:77、SEQ ID NO:78、SEQ ID NO:79、SEQ IDNO:80、SEQ ID NO:81、SEQ ID NO:82、SEQ ID NO:83、SEQ ID NO:84、SEQ ID NO:85、SEQ IDNO:86、SEQ ID NO:87、SEQ ID NO:88、SEQ ID NO:89、SEQ ID NO:90、SEQ ID NO:91、SEQ IDNO:92、SEQ ID NO:93、SEQ ID NO:94、SEQ ID NO:95、SEQ ID NO:96、SEQ ID NO:97、SEQ IDNO:98、SEQ ID NO:99、SEQ ID NO:100、SEQ ID NO:101、SEQ ID NO:102、SEQ ID NO:103、SEQ ID NO:104、SEQ ID NO:105、SEQ ID NO:106、SEQ ID NO:107、SEQ ID NO:108、SEQ IDNO:109、SEQ ID NO:110或SEQ ID NO:111的组,优选地选自SEQ ID NO:10、SEQ ID NO:11、SEQ ID NO:15、SEQ ID NO:27、SEQ ID NO:28、SEQ ID NO:29、SEQ ID NO:42、SEQ ID NO:62或SEQ ID NO:63的组中的多肽。在一些实施方式中,多肽定位于植物细胞的至少一种叶绿体的叶绿体被膜或叶绿体类囊体膜。在一些实施方式中,植物细胞是叶片叶肉细胞。在一些实施方式中,多肽在植物的至少70%的叶片叶肉细胞中表达。在一些实施方式中,植物是豇豆、大豆、木薯、水稻、豆子(soy)、小麦或其他C3作物植物。在一些实施方式中,植物不是玉米、高粱或其他C4作物植物。
在任何以上实施方式的一些实施方式中,调节内源碳酸酐酶的表达。在一些实施方式中,调节的表达可以是增加的表达、减少的表达、在不同位置的表达或其任何组合。
本公开的某些方面涉及产生具有增加的碳利用效率的植物的方法,其中方法的步骤是:a)将基因改变引入植物,导致增加或提供碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力或者增加或提供碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力,从而增加植物的碳利用效率。
在一些方面中,本公开涉及产生具有增加的水利用效率的植物的方法,其中方法的步骤是:a)将基因改变引入植物,导致增加或提供碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力或者增加或提供碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力,从而增加植物的水利用效率。
在一些方面中,本公开涉及产生具有增加的氮利用效率的植物的方法,其中方法的步骤是:a)将基因改变引入植物,导致增加或提供碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力或者增加或提供碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力,从而增加植物的氮利用效率。
在一些方面中,本公开涉及产生具有减少的光抑制的植物的方法,其中方法的步骤是:a)将基因改变引入植物,导致增加或提供碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力或者增加或提供碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力,从而减少植物的光抑制。
在一些方面中,本公开涉及产生具有增加的生长或生产率(productivity)的植物的方法,其中方法的步骤是:a)将基因改变引入植物,导致增加或提供碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力或者增加或提供碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力,从而增加植物的生长或生产率。
在任何以上方法的一些实施方式中,调节内源碳酸酐酶的表达。在一些实施方式中,调节的表达可以是增加的表达、减少的表达、在不同位置的表达或其任何组合。
在一些方面中,本公开涉及栽培具有增加的碳利用效率的植物的方法,其中方法的步骤是:a)提供具有增加或提供碳酸氢盐跨过膜的能力的一个或多个基因改变的种子,其中种子产生具有碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力的植物或者具有碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力的植物;b)在一定条件下栽培植物,其中与在相同条件下生长的缺少一个或多个基因改变的植物相比,碳酸氢盐跨过膜的能力增加了碳利用效率。
在一些方面中,本公开涉及栽培具有增加的水利用效率的植物的方法,其中方法的步骤是:a)提供具有增加或提供碳酸氢盐跨过膜的能力的一个或多个基因改变的种子,其中种子产生具有碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力的植物或者具有碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力的植物;b)在一定条件下栽培植物,其中与在相同条件下生长的缺少一个或多个基因改变的植物相比,碳酸氢盐跨过膜的能力增加水利用效率。
在一些方面中,本公开涉及栽培具有增加的氮利用效率的植物的方法,其中方法的步骤是:a)提供具有增加或提供碳酸氢盐跨过膜的能力的一个或多个基因改变的种子,其中种子产生具有碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力的植物或具有碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力的植物;b)在一定条件下栽培植物,其中与在相同条件下生长的缺少一个或多个基因改变的植物相比,碳酸氢盐跨过膜的能力增加氮利用效率。
在一些方面中,本公开涉及栽培具有减少的光抑制的植物的方法,其中方法的步骤是:a)提供具有增加或提供碳酸氢盐跨过膜的能力的一个或多个基因改变的种子,其中种子产生具有碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力的植物或者具有碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力的植物;b)在一定条件下栽培植物,其中与在相同条件下生长的缺少一个或多个基因改变的植物相比,碳酸氢盐跨过膜的能力减少光抑制。
在一些方面中,本公开涉及栽培具有增加的生长或生产率的植物的方法,其中方法的步骤是:a)提供具有增加或提供碳酸氢盐跨过膜的能力的一个或多个基因改变的种子,其中种子产生具有碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力的植物或者具有碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力的植物;b)在一定条件下栽培植物,其中与在相同条件下生长的缺少一个或多个基因改变的植物相比,碳酸氢盐跨过膜的能力增加生长或生产率。
在一些方面中,本公开涉及栽培具有增加的碳利用效率的植物的方法,其中方法的步骤是:a)提供具有增加或提供碳酸氢盐跨过膜的能力的一个或多个基因改变的组织培养物或原生质体;b)使组织培养物或原生质体再生为小植株;c)使小植株生长为植物,其中植物具有碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力或碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力;d)将植物移植到一定条件中,其中与在相同条件下生长的缺少一个或多个基因改变的植物相比,碳酸氢盐跨过膜的能力增加碳利用效率。
在一些方面中,本公开涉及栽培具有增加的水利用效率的植物的方法,其中方法的步骤是:a)提供具有增加或提供碳酸氢盐跨过膜的能力的一个或多个基因改变的组织培养物或原生质体;b)使组织培养物或原生质体再生为小植株;c)使小植株生长为植物,其中植物具有碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力或碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力;d)将植物移植到一定条件中,其中与在相同条件下生长的缺少一个或多个基因改变的植物相比,碳酸氢盐跨过膜的能力增加水利用效率。
在一些方面中,本公开涉及栽培具有增加的氮利用效率的植物的方法,其中方法的步骤是:a)提供具有增加或提供碳酸氢盐跨过膜的能力的一个或多个基因改变的组织培养物或原生质体;b)使组织培养物或原生质体再生为小植株;c)使小植株生长为植物,其中植物具有碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力或碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力;d)将植物移植到一定条件中,其中与在相同条件下生长的缺少一个或多个基因改变的植物相比,碳酸氢盐跨过膜的能力增加氮利用效率。
在一些方面中,本公开涉及栽培具有减少的光抑制的植物的方法,其中方法的步骤是:a)提供具有增加或提供碳酸氢盐跨过膜的能力的一个或多个基因改变的组织培养物或原生质体;b)使组织培养物或原生质体再生为小植株;c)使小植株生长为植物,其中植物具有碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力或碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力;d)将植物移植到一定条件中,其中与在相同条件下生长的缺少一个或多个基因改变的植物相比,碳酸氢盐跨过膜的能力减少光抑制。
在一些方面中,本公开涉及栽培具有增加的生长或生产率的植物的方法,其中方法的步骤是:a)提供具有增加或提供碳酸氢盐跨过膜的能力的一个或多个基因改变的组织培养物或原生质体;b)使组织培养物或原生质体再生为小植株;c)使小植株生长为植物,其中植物具有碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力或碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力;d)将植物移植到一定条件中,其中与在相同条件下生长的缺少一个或多个基因改变的植物相比,碳酸氢盐跨过膜的能力增加生长或生产率。
在任何以上方法的一些实施方式中,种子、组织培养物或原生质体具有调节内源碳酸酐酶的表达的一个或多个基因改变。在一些实施方式中,调节的表达可以是增加的表达、减少的表达、在不同位置的表达或其任何组合。
在任何以上方法的一些实施方式中,碳酸氢盐跨过膜的能力的增加或提供是选自与SEQ ID NO:1具有至少95%序列同一性的第一多肽、与SEQ ID NO:2具有至少95%序列同一性的第二多肽、与SEQ ID NO:3具有至少95%序列同一性的第三多肽或其任何组合的组中的至少一种多肽的表达的结果。在任何以上方法的一些实施方式中,碳酸氢盐跨过膜的能力的增加或提供是选自SEQ ID NO:4、SEQ ID NO:5、SEQ ID NO:6、SEQ ID NO:7、SEQ IDNO:8、SEQ ID NO:9、SEQ ID NO:10、SEQ ID NO:11、SEQ ID NO:12、SEQ ID NO:13、SEQ IDNO:14、SEQ ID NO:15、SEQ ID NO:16、SEQ ID NO:17、SEQ ID NO:18、SEQ ID NO:19、SEQ IDNO:20、SEQ ID NO:21、SEQ ID NO:22、SEQ ID NO:23、SEQ ID NO:24、SEQ ID NO:25、SEQ IDNO:26、SEQ ID NO:27、SEQ ID NO:28、SEQ ID NO:29、SEQ ID NO:30、SEQ ID NO:31、SEQ IDNO:32、SEQ ID NO:33、SEQ ID NO:34、SEQ ID NO:35、SEQ ID NO:36、SEQ ID NO:37、SEQ IDNO:38、SEQ ID NO:39、SEQ ID NO:40、SEQ ID NO:41、SEQ ID NO:42、SEQ ID NO:43、SEQ IDNO:44、SEQ ID NO:45、SEQ ID NO:46、SEQ ID NO:47、SEQ ID NO:48、SEQ ID NO:49、SEQ IDNO:50、SEQ ID NO:51、SEQ ID NO:52、SEQ ID NO:53、SEQ ID NO:54、SEQ ID NO:55、SEQ IDNO:56、SEQ ID NO:57、SEQ ID NO:58、SEQ ID NO:59、SEQ ID NO:60、SEQ ID NO:61、SEQ IDNO:62、SEQ ID NO:63、SEQ ID NO:64、SEQ ID NO:65、SEQ ID NO:66、SEQ ID NO:67、SEQ IDNO:68、SEQ ID NO:69、SEQ ID NO:70、SEQ ID NO:71、SEQ ID NO:72、SEQ ID NO:73、SEQ IDNO:74、SEQ ID NO:75、SEQ ID NO:76、SEQ ID NO:77、SEQ ID NO:78、SEQ ID NO:79、SEQ IDNO:80、SEQ ID NO:81、SEQ ID NO:82、SEQ ID NO:83、SEQ ID NO:84、SEQ ID NO:85、SEQ IDNO:86、SEQ ID NO:87、SEQ ID NO:88、SEQ ID NO:89、SEQ ID NO:90、SEQ ID NO:91、SEQ IDNO:92、SEQ ID NO:93、SEQ ID NO:94、SEQ ID NO:95、SEQ ID NO:96、SEQ ID NO:97、SEQ IDNO:98、SEQ ID NO:99、SEQ ID NO:100、SEQ ID NO:101、SEQ ID NO:102、SEQ ID NO:103、SEQ ID NO:104、SEQ ID NO:105、SEQ ID NO:106、SEQ ID NO:107、SEQ ID NO:108、SEQ IDNO:109、SEQ ID NO:110或SEQ ID NO:111的组,优选地选自SEQ ID NO:10、SEQ ID NO:11、SEQ ID NO:15、SEQ ID NO:27、SEQ ID NO:28、SEQ ID NO:29、SEQ ID NO:42、SEQ ID NO:62或SEQ ID NO:63的组中的至少一种多肽的表达的结果。在任何以上方法的一些实施方式中,植物是豇豆、大豆、木薯、水稻、豆子、小麦或其他C3作物植物。在任何以上方法的一些实施方式中,植物不是玉米、高粱或其他C4作物植物。
本公开的某些方面涉及含有一个或多个基因改变的基因改变的藻类,所述基因改变导致增加藻类将碳酸氢盐转运进入藻类的叶绿体的腔的能力。在一些实施方式中,增加碳酸氢盐转运能力是过表达来自与SEQ ID NO:1具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性或至少95%序列同一性的第一多肽,与SEQ ID NO:2具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性或至少95%序列同一性的第二多肽,与SEQ ID NO:3具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性或至少95%序列同一性的第三多肽或其任何组合的组中的至少一种绿藻的bestrophin多肽的结果。在一些实施方式中,增加碳酸氢盐转运能力是过表达来自与SEQ ID NO:1具有至少95%序列同一性的第一多肽、与SEQID NO:2具有至少95%序列同一性的第二多肽、与SEQ ID NO:3具有至少95%序列同一性的第三多肽或其任何组合的组中的至少一种绿藻的bestrophin多肽的结果。在一些实施方式中,多肽定位于叶绿体类囊体膜。在一些实施方式中,至少当藻类在<100ppm二氧化碳(空气中<0.01%[v/v]CO2)条件下时,过表达多肽。
在一些方面中,本公开涉及具有增加的碳酸氢盐转运的绿藻或其部分,其含有包含至少一种绿藻的bestrophin多肽的编码序列的修饰的核酸序列;其中过表达bestrophin多肽;其中bestrophin多肽定位于叶绿体类囊体膜;并且其中当在<100ppm二氧化碳(空气中<0.01%[v/v]CO2)条件下栽培藻类时,产率、生长速率或生物量大于来自不过表达至少一种bestrophin多肽的相应的野生型(WT)藻类或其WT部分的产率、生长速率或生物量或者产率、生长速率或生物量基本上类似于来自在<100ppm二氧化碳(空气中<0.01%[v/v]CO2)条件下栽培的不过表达至少一种bestrophin多肽的相应的WT藻类或相应的其WT部分的产率、生长速率或生物量。在一些实施方式中,至少一种绿藻的bestrophin多肽选自与SEQ IDNO:1具有至少95%序列同一性的第一多肽、与SEQ ID NO:2具有至少95%序列同一性的第二多肽、与SEQ ID NO:3具有至少95%序列同一性的第三多肽或其任何组合的组。
在一些方面中,本公开涉及在<100ppm二氧化碳(空气中<0.01%[v/v]CO2)条件下具有增加的生长的绿藻或其部分,其含有包含至少一种绿藻的bestrophin多肽的编码序列的修饰的核酸序列;其中过表达bestrophin多肽;其中bestrophin多肽定位于叶绿体类囊体膜;并且其中当在<100ppm二氧化碳(空气中<0.01%[v/v]CO2)条件下栽培藻类时,产率、生长速率或生物量大于来自不过表达至少一种bestrophin多肽的相应的野生型(WT)藻类或其WT部分的产率、生长速率或生物量或者产率、生长速率或生物量基本上类似于来自在<100ppm二氧化碳(空气中<0.01%[v/v]CO2)条件下栽培的不过表达至少一种bestrophin多肽的相应的WT藻类或相应的其WT部分的产率、生长速率或生物量。在一些实施方式中,至少一种绿藻的bestrophin多肽选自与SEQ ID NO:1具有至少95%序列同一性的第一多肽、与SEQ ID NO:2具有至少95%序列同一性的第二多肽、与SEQ ID NO:3具有至少95%序列同一性的第三多肽或其任何组合的组。
在任何以上海藻的一些实施方式中,绿藻选自莱茵衣藻、Chlamydomonaseustigma、Volvox carteri f.nagariensis,和胸状盘藻(Gonium pectorale)的组中。
在一些方面中,本公开涉及产生具有增加的碳利用效率的藻类的方法,其中方法的步骤是:a)将基因改变引入藻类,包括增加将碳酸氢盐转运进入藻类的叶绿体的腔的能力,从而增加藻类的碳利用效率。在一些实施方式中,转运能力的获得是过表达选自与SEQID NO:1具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性或至少95%序列同一性的第一多肽,与SEQ ID NO:2具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性或至少95%序列同一性的第二多肽,与SEQ ID NO:3具有至少70%序列同一性、至少75%序列同一性、至少80%序列同一性、至少85%序列同一性、至少90%序列同一性或至少95%序列同一性的第三多肽或其任何组合的组中的至少一种绿藻的bestrophin多肽的结果。在一些实施方式中,增加碳酸氢盐转运能力是过表达选自与SEQID NO:1具有至少95%序列同一性的第一多肽、与SEQ ID NO:2具有至少95%序列同一性的第二多肽、与SEQ ID NO:3具有至少95%序列同一性的第三多肽或其任何组合的组中的至少一种绿藻的bestrophin多肽的结果。
附图说明
图1A-1D示出莱茵衣藻基因(BST1、BST2和BST3)和蛋白质(BST1、BST2和BST3)彼此的相似性,以及BST1-3与其他bestrophin家族蛋白质的相似性。图1A示出BST1、BST2和BST3基因的示意图,其中浅灰色框表示外显子、细灰线表示内含子、粗灰线表示非翻译区(UTR),并且重叠线(在外显子和内含子上重叠)指示由三个基因共享的共同区域。图1B示出莱茵衣藻基因组上的BST1、BST2和BST3基因的位置和定向。图1C示出BST1(SEQ ID NO:1)、BST2(SEQ ID NO:2)和BST3(SEQ ID NO:3)蛋白质的氨基酸比对,其中底行中的星号指示在全部三个蛋白中相同的氨基酸。图1D示出维管植物(从顶部的第二组)、非维管植物(从顶部的第三组)、硅藻(从顶部的第四组)和绿藻(在底部的组)中莱茵衣藻BST1、BST2和BST3同系物的蛋白质序列的系统进化树。
图2A-2B示出莱茵衣藻基因BST1、BST2和BST3的转录物分析。图2A示出显示野生型菌株D66和cia5细胞中在低CO2(空气中<0.04%CO2)与高CO2(空气中5%(v/v)CO2)中积聚BST1-3的半定量RT-PCR。图2B示出显示从在高CO2(空气中5%CO2(v/v))中生长的细胞和转换至低CO2(空气中<0.04%CO2)2小时(2h)、4小时(4h)、6小时(6h)或12小时(12h)的细胞中获得的cDNA中BST1-3的表达的半定量RT-PCR时间进程。在图2A和图2B二者中肌动蛋白用作载量对照,并且显示的结果来自两次重复之一。
图3A-3B示出指示莱茵衣藻中BST1-Venus、BST2-Venus和BST3-Venus融合蛋白质的定位的共焦显微镜荧光蛋白图像。图3A示出莱茵衣藻中BST1-Venus、BST2-Venus和BST3-Venus融合蛋白质表达的定位(“Venus”列)、叶绿体类囊体的定位(“叶绿体”列)和二者相对于彼此的定位(“融合”列)。比例尺是5μm。图3B示出来自图3A中“BST1”行的蛋白核的放大图像。箭头突出了穿透蛋白核在类囊体小管中的蛋白核的内部可看到的BST1-Venus荧光的地方。比例尺是1μm。图3A和图3B二者的图像是多次重复的代表性图像。
图4示出与野生型菌株D66相比,RNAi敲低菌株bsti-1和bsti-2中BST1-3表达的qRT-PCR分析的结果。误差条指示三次生物学重复的标准误差。
图5A-5C示出在不同pH和CO2条件下,与野生型菌株D66相比,RNAi敲低菌株bsti-1以及pmp1和cia3突变体菌株的生长表型分析的结果。纵向的点表示三种不同细胞浓度;10,000个细胞、5,000个细胞和2,500个细胞。图5A示出在非常低CO2(空气中0.01%CO2(v/v))条件和7或8.4的pH下,菌株的生长表型分析的结果。图5B示出在低CO2(空气中0.04%CO2(v/v))和7或8.4的pH下,菌株的生长表型分析的结果。图5C示出在高CO2(空气中5%CO2(v/v))条件和7或8.4的pH下,菌株的生长表型分析的结果。重复三次生长表型分析实验并且显示的结果是代表性的。
图6A-6F示出BST-RNAi系1和2,即bsti-1和bsti-2,和D66的光合氧释放活性。图6A示出对于在pH 8.4适应低CO2(<0.04%CO2)12小时的bsti-1和D66,从O2释放对比Ci曲线计算的K0.5(Ci)值(半最大氧释放所需的Ci浓度)。图6B示出对于在pH 8.4适应低CO2(<0.04%CO2)12小时的bsti-1和D66,将在不同Ci量和在不同pH值测量的氧释放活性绘制为曲线。图6C示出对于在pH 7.8适应低CO2(<0.04%CO2)12小时的bsti-1、bsti-2和D66,从O2释放对比Ci曲线计算的K0.5(Ci)值(半最大氧释放所需的Ci浓度)。符号“*”指示K0.5(Ci)的差异是显著的(通过student t检验P<0.05)。图6D示出对于在pH 7.8适应低CO2(<0.04%CO2)12小时的bsti-1、bsti-2和D66,将在不同Ci量和在不同pH值测量的氧释放活性绘制为曲线。图6E示出对于在pH 7.8适应高CO2(>5%CO2)12小时的bsti-1和D66,从O2释放对比Ci曲线计算的K0.5(Ci)值(半最大氧释放所需的Ci浓度)。图6F示出对于在pH 7.8适应高CO2(>5%CO2)12小时的bsti-1和D66,将在不同Ci量和在不同pH值测量的氧释放活性绘制为曲线。对于图6A-6F,以每种Ci浓度进行一式三份运行,并且误差条表示三个生物学重复且基于标准偏差。所有菌株的Vmax设置至100%氧释放活性。
图7A-7D示出bsti-1和D66的无机碳摄取。图7A示出在pH 7.8细胞内(内部)Ci积聚的时间过程。图7B示出在pH 7.8的CO2(Ci)固定的时间过程。图7C示出在pH 8.4细胞内Ci积聚的时间过程。图7D示出在pH 8.4的CO2固定的时间过程。细胞在升高的CO2(空气中5%CO2)中生长,并且然后在测定之前适应低CO2(<0.04%CO2)12小时。在运行测定前,收获细胞且耗尽内源Ci,并且每个时间点运行一式三份样品。在图7A-7D中的误差条表示三个生物学重复。
图8A-8C示出在来自衣藻文库项目(CLiP)的突变体bst3中敲除的BST3。图8A示出BST3基因的示意图,其中外显子描绘为灰色框,内含子描绘为黑色线和非翻译区描绘为深灰色框。插入物的位置显示为浅灰色三角形,并且检测插入物的引物显示为小的黑色箭头。图8B示出使用图8A中显示的引物的PCR反应的结果以确认bst3中插入物的位置。泳道1,BST3F和BST3R引物使用WT菌株D66 DNA作为模板;泳道2,CIB1F和BST3F引物使用bst3 DNA作为模板;泳道3,CIB1R和BST3R引物使用bst3 DNA作为模板;泳道4,BST3F和BST3R引物使用bst3 DNA作为模板。泳道1和4之间的尺寸差异显示存在1800bp盒。图8C示出显示在D66和bst3细胞中低CO2(空气中<0.04%CO2)对比高CO2(空气中5%(v/v)CO2)的BST1-3积聚的半定量RT-PCR。如通过红色框指示,BST3在bst3细胞中不表达。肌动蛋白用作载量对照。
图9A-9D示出与WT菌株D66相比,敲除菌株bst3的生长和无机碳亲和力的测量。图9A示出在低CO2(<0.04%CO2)的pH 8.6中六天bst3和WT的生长,其使用OD730测量。图9B示出在低CO2(<0.04%CO2)的pH 8.6在pH 8.6中六天bst3和WT的生长,其使用叶绿体评估在波长645和663处测量。图9C示出对于bst3和D66,将在pH 7.4测量的氧释放活性绘制为曲线。以每种Ci浓度进行一式三份运行。图9D示出对于在pH 7.4bst3和D66,从O2释放对比Ci曲线计算的K0.5(Ci)值(半最大氧释放所需的Ci浓度)。
图10A-10D示出莱茵衣藻BST1、BST2和BST3的蛋白质结构模型。图10A显示作为单体的莱茵衣藻BST1-3和肺炎克雷伯杆菌(Klebsiella pneumonia)bestrophin(Kpbest)的结构模型,具有在选择性孔内层的保守的残基,其显示为伸出形状并标记。图10B示出用局部QMEAN评分(结构质量评估评分功能)加阴影的BST1均五聚物模型的二级结构。图10C示出以黑色在BST1均五聚物模型上画的通道腔的轮廓。来自图10A中BST1模型的保守的残基描绘为伸出形状延伸至通道腔内。图10D示出BST1均五聚物模型上的计算的静电势。静电势以-4kT/e(负极)至+4kT/e(正极)的电势范围展示。
图11示出用于无机碳(Ci)转运的当前的莱茵衣藻CCM模型。BST1、BST2和BST3显示为定位于叶绿体类囊体膜的碳酸氢盐-转运通道(浅灰色、灰色和深灰色矩形)。
具体实施方式
以下描述陈述示例性方法、参数等。然而,应认识到,这种描述不旨为本公开的范围的限制,而是作为示例性实施方式的描述提供。
基因改变的植物和种子
本公开的某些方面涉及含有一个或多个基因改变的基因改变的植物或其部分,所述基因改变增加或提供碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力。在一些方面中,本公开涉及含有一个或多个基因改变的基因改变的植物或其部分,所述基因改变增加或提供碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力。在一些实施方式中,碳酸氢盐膜跨过能力的获得是至少一种绿藻的bestrophin多肽的表达的结果。在一些实施方式中,碳酸氢盐膜跨过能力的获得是两种或三种绿藻的bestrophin多肽的表达的结果。在一些实施方式中,碳酸氢盐膜跨过能力的获得是四种或更多种(例如,五种、六种、七种、八种、九种、十种)绿藻的bestrophin多肽的表达的结果。在一些实施方式中,绿藻的bestrophin多肽是SEQ ID NO:1(即,BESTROPHIN1,BST1,Cre16.g662600.t1.2)、SEQ ID NO:2(即,BESTROPHIN2,BST2,Cre16.g663400.t2.1)或SEQID NO:3(即,BESTROPHIN3,BST3,Cre16.g663450.t1.2)。在一些实施方式中,碳酸氢盐跨过膜的能力的增加或提供是选自与SEQ ID NO:1具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%或至少95%序列同一性的第一多肽,与SEQ ID NO:2具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%或至少95%序列同一性的第二多肽,与SEQ ID NO:3具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%或至少95%序列同一性的第三多肽或其任何组合的组中的至少一种多肽的表达的结果。在一些实施方式中,碳酸氢盐跨过膜的能力的增加或提供是选自与SEQ ID NO:1具有至少95%序列同一性的第一多肽、与SEQ ID NO:2具有至少95%序列同一性的第二多肽、与SEQ ID NO:3具有至少95%序列同一性的第三多肽或其任何组合的组中的多肽的表达的结果。
术语“BST1”,以及其大写和斜体版本,指绿藻的基因和蛋白质,如本文描述的。在一些实施方式中,该术语可指莱茵衣藻基因和蛋白质,如本文描述的。在其他实施方式中,该术语可指也转运碳酸氢盐的任何绿藻物种的基因和蛋白质的一种或多种同源物或直系同源物。在一些实施方式中,该术语可指任何绿藻物种的基因和蛋白质的一种或多种旁系同源物。在一些实施方式中,绿藻物种是莱茵衣藻、Chlamydomonas eustigma、Volvoxcarteri f.nagariensis或胸状盘藻。SEQ ID NO:1提供了莱茵衣藻BST1蛋白质。当用斜体的所有小写字母表示时,意指基因/蛋白质的突变体(例如,敲除)版本。在莱茵衣藻中,突变体版本可为单个基因/蛋白质。在其他绿藻物种中,突变体版本可为基因/蛋白质的一个、一些或所有同源物、直系同源物和/或旁系同源物。
术语“BST2”,以及其大写和斜体版本,指绿藻的基因和蛋白质,如本文描述的。在一些实施方式中,该术语可指莱茵衣藻基因和蛋白质,如本文描述的。在其他实施方式中,该术语可指也转运碳酸氢盐的任何绿藻物种的基因和蛋白质的一种或多种同源物或直系同源物。在一些实施方式中,该术语可指任何绿藻物种的基因和蛋白质的一种或多种旁系同源物。在一些实施方式中,绿藻物种是莱茵衣藻、Chlamydomonas eustigma、Volvoxcarteri f.nagariensis或胸状盘藻。SEQ ID NO:2提供莱茵衣藻BST2蛋白质。当用斜体的所有小写字母表示时,意指基因/蛋白质的突变体(例如,敲除)版本。在莱茵衣藻中,突变体版本可为单个基因/蛋白质。在其他绿藻物种中,突变体版本可为基因/蛋白质的一个、一些或所有同源物、直系同源物和/或旁系同源物。
术语“BST3”,以及其大写和斜体版本,指绿藻的基因和蛋白质,如本文描述的。在一些实施方式中,该术语可指莱茵衣藻基因和蛋白质,如本文描述的。在其他实施方式中,该术语可指也转运碳酸氢盐的任何绿藻物种的基因和蛋白质的一种或多种同源物或直系同源物。在一些实施方式中,该术语可指任何绿藻物种的基因和蛋白质的一种或多中旁系同源物。在一些实施方式中,绿藻物种是莱茵衣藻、Chlamydomonas eustigma、Volvoxcarteri f.nagariensis或胸状盘藻。SEQ ID NO:3提供莱茵衣藻BST3蛋白质。当用斜体的所有小写字母表示时,意指基因/蛋白质的突变体(例如,敲除)版本。在莱茵衣藻中,突变体版本可为单个基因/蛋白质。在其他绿藻物种中,突变体版本可为基因/蛋白质的一个、一些或所有同源物、直系同源物和/或旁系同源物。
在一些实施方式中,碳酸氢盐跨过膜的能力的增加或提供是来自以下组的多肽或与以下组之一具有高百分比同一性的多肽的表达的结果:SEQ ID NO:4、SEQ ID NO:5、SEQID NO:6、SEQ ID NO:7、SEQ ID NO:8、SEQ ID NO:9、SEQ ID NO:10、SEQ ID NO:11、SEQ IDNO:12、SEQ ID NO:13、SEQ ID NO:14、SEQ ID NO:15、SEQ ID NO:16、SEQ ID NO:17、SEQ IDNO:18、SEQ ID NO:19、SEQ ID NO:20、SEQ ID NO:21、SEQ ID NO:22、SEQ ID NO:23、SEQ IDNO:24、SEQ ID NO:25、SEQ ID NO:26、SEQ ID NO:27、SEQ ID NO:28、SEQ ID NO:29、SEQ IDNO:30、SEQ ID NO:31、SEQ ID NO:32、SEQ ID NO:33、SEQ ID NO:34、SEQ ID NO:35、SEQ IDNO:36、SEQ ID NO:37、SEQ ID NO:38、SEQ ID NO:39、SEQ ID NO:40、SEQ ID NO:41、SEQ IDNO:42、SEQ ID NO:43、SEQ ID NO:44、SEQ ID NO:45、SEQ ID NO:46、SEQ ID NO:47、SEQ IDNO:48、SEQ ID NO:49、SEQ ID NO:50、SEQ ID NO:51、SEQ ID NO:52、SEQ ID NO:53、SEQ IDNO:54、SEQ ID NO:55、SEQ ID NO:56、SEQ ID NO:57、SEQ ID NO:58、SEQ ID NO:59、SEQ IDNO:60、SEQ ID NO:61、SEQ ID NO:62、SEQ ID NO:63、SEQ ID NO:64、SEQ ID NO:65、SEQ IDNO:66、SEQ ID NO:67、SEQ ID NO:68、SEQ ID NO:69、SEQ ID NO:70、SEQ ID NO:71、SEQ IDNO:72、SEQ ID NO:73、SEQ ID NO:74、SEQ ID NO:75、SEQ ID NO:76、SEQ ID NO:77、SEQ IDNO:78、SEQ ID NO:79、SEQ ID NO:80、SEQ ID NO:81、SEQ ID NO:82、SEQ ID NO:83、SEQ IDNO:84、SEQ ID NO:85、SEQ ID NO:86、SEQ ID NO:87、SEQ ID NO:88、SEQ ID NO:89、SEQ IDNO:90、SEQ ID NO:91、SEQ ID NO:92、SEQ ID NO:93、SEQ ID NO:94、SEQ ID NO:95、SEQ IDNO:96、SEQ ID NO:97、SEQ ID NO:98、SEQ ID NO:99、SEQ ID NO:100、SEQ ID NO:101、SEQID NO:102、SEQ ID NO:103、SEQ ID NO:104、SEQ ID NO:105、SEQ ID NO:106、SEQ ID NO:107、SEQ ID NO:108、SEQ ID NO:109、SEQ ID NO:110或SEQ ID NO:111,优选地选自由SEQID NO:10、SEQ ID NO:11、SEQ ID NO:15、SEQ ID NO:27、SEQ ID NO:28、SEQ ID NO:29、SEQID NO:42、SEQ ID NO:62或SEQ ID NO:63组成的组。两种多核苷酸或多肽的上下文中短语“高百分比同一的”、“高百分比同一性”或“高序列同一性”和其语法变体指当就最大对应进行比较和对比时,如使用序列比较算法或通过视觉检查测量的,具有至少约80%同一性,至少约81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%、99%或100%核苷酸或氨基酸同一性的两种或更多种序列或亚序列。在示例性实施方式中,在至少约16个核苷酸或氨基酸的长度的序列区域上存在高百分比同一性。在另一示例性实施方式中,在至少约50个核苷酸或氨基酸的长度的序列区域上存在高百分比同一性。在仍另一示例性实施方式中,在至少约100个核苷酸或氨基酸或更长的长度的序列区域上存在高百分比同一性。在一个示例性实施方式中,在多核苷酸或多肽序列的整个长度上,序列是高百分比同一的。
在一些实施方式中,多肽定位于植物细胞内至少一种叶绿体的叶绿体被膜或类囊体膜。在一些实施方式中,多肽能够移动碳酸氢盐(HCO3 -)跨过膜。在一些实施方式中,多肽能够移动氯根阴离子(Cl-)跨过膜。在一些实施方式中,多肽能够移动HCO3 -和Cl-二者跨过膜。在一些实施方式中,多肽能够移动带负电的离子跨过膜。在一些实施方式中,植物细胞是叶片叶肉细胞。在一些实施方式中,多肽在植物的至少70%的叶片叶肉细胞中表达。在一些实施方式中,多肽寡聚以形成五聚物。在一些实施方式中,多肽寡聚以形成均五聚物。在一些实施方式中,五聚物或均五聚物具有主要具有负静水势(negative hydrostaticpotential)的进入袋和具有中性/正电荷的选择性孔。在一些实施方式中,均五聚物转运带负电的离子。
在一些方面中,本公开涉及具有增加的碳利用效率的植物或其部分,其含有具有植物或其部分中绿藻的bestrophin多肽的至少一种编码序列的至少一种修饰核酸的序列,其中在植物或其部分中表达bestrophin多肽,和其中当在环境二氧化碳条件下栽培植物时,产率、生长速率或生物量大于来自不过表达bestrophin多肽的相应的野生型(WT)植物或相应的其WT部分的产率、生长速率或生物量或者产率、生长速率或生物量基本上类似于来自在环境二氧化碳条件下栽培的不过表达bestrophin多肽的相应的野生型(WT)植物或相应的其WT部分的产率、生长速率或生物量。如本文使用的,术语“环境二氧化碳”指没有任何添加或去除的CO2的空气的二氧化碳含量。在一些实施方式中,环境二氧化碳条件是400-500ppm、400-550ppm、400-600ppm、400-650ppm、400-700ppm、450-500ppm、450-550ppm、450-600ppm、450-650ppm、450-700ppm、500-550ppm、500-600ppm、500-650ppm、500-700ppm、550-600ppm、550-650ppm、550-700ppm、600-650ppm、600-700ppm或650-700ppm的二氧化碳条件。如本文使用的,术语“碳利用效率”可指并入植物的生物量的从环境获取的碳的比例。碳利用效率可通过本领域中任何已知的方法(例如,由植物吸收的碳的总量减去通过植物呼吸损失的碳的量,然后将该值除以由植物吸收的碳的总量等)测量。在一些实施方式中,bestrophin多肽定位于植物细胞的至少一种叶绿体的叶绿体被膜或叶绿体类囊体膜。在一些实施方式中,植物细胞是叶片叶肉细胞。在一些实施方式中,多肽在植物的至少70%的叶片叶肉细胞中表达。
在一些方面中,本公开涉及具有增加的水利用效率的植物或其部分,其含有具有植物或其部分中绿藻的bestrophin多肽的至少一种编码序列的至少一种修饰的核酸序列,其中在植物或其部分中表达bestrophin多肽,和其中当在环境二氧化碳条件下栽培植物时,产率、生长速率或生物量大于来自不过表达bestrophin多肽的相应的野生型(WT)植物或相应的其WT部分的产率、生长速率或生物量或者产率、生长速率或生物量基本上类似于来自在环境二氧化碳条件下栽培的不过表达bestrophin多肽的相应的野生型(WT)植物或相应的其WT部分的产率、生长速率或生物量。如本文使用的,术语“水利用效率”指植物中碳同化与水消耗的比率。水利用效率的测量包括但不限于固有的水利用效率和即时的水利用效率。即时的水利用效率可通过确定植物碳同化和植物蒸腾作用之间的比率计算。固有的水利用效率可通过确定植物碳同化和植物气孔通导率之间的比率来计算。碳同化的测量可包括但不限于植物光合速率、产率和生物量。在一些实施方式中,bestrophin多肽定位于植物细胞的至少一种叶绿体的叶绿体被膜或叶绿体类囊体膜。在一些实施方式中,植物细胞是叶片叶肉细胞。在一些实施方式中,多肽在植物的至少70%的叶片叶肉细胞中表达。
在一些方面中,本公开涉及具有增加的氮利用效率的植物或其部分,其含有具有植物或其部分中绿藻的bestrophin多肽的至少一种编码序列的至少一种修饰的核酸序列,其中在植物或其部分中表达bestrophin多肽,和其中当在环境二氧化碳条件下栽培植物时,产率、生长速率或生物量大于来自不过表达bestrophin多肽的相应的野生型(WT)植物或相应的其WT部分的产率、生长速率或生物量或者产率、生长速率或生物量基本上类似于来自在环境二氧化碳条件下栽培的不过表达bestrophin多肽的相应的野生型(WT)植物或相应的其WT部分的产率、生长速率或生物量。如本文使用的,术语“氮利用效率”指植物用于代谢使用的氮与供应给植物的总氮的比率。氮利用效率可通过本领域中任何已知的方法(例如,15N同位素标记、农艺效率、表观氮回收)测量。供应给植物的氮的来源包括但不限于土壤中含有的氮、由固氮菌供应的氮和肥料中含有的氮。在一些实施方式中,bestrophin多肽定位于植物细胞的至少一种叶绿体的叶绿体被膜或叶绿体类囊体膜。在一些实施方式中,植物细胞是叶片叶肉细胞。在一些实施方式中,多肽在植物的至少70%的叶片叶肉细胞中表达。
在一些方面中,本公开涉及具有减少的光抑制的植物或其部分,其含有具有植物或其部分中绿藻的bestrophin多肽的至少一种编码序列的至少一种修饰的核酸序列,其中在植物或其部分中表达bestrophin多肽,和其中当在环境二氧化碳条件下栽培植物时,产率、生长速率或生物量大于来自不过表达bestrophin多肽的相应的野生型(WT)植物或相应的其WT部分的产率、生长速率或生物量或者产率、生长速率或生物量基本上类似于来自在环境二氧化碳条件下栽培的不过表达bestrophin多肽的相应的野生型(WT)植物或相应的其WT部分的产率、生长速率或生物量。如本文使用的,术语“光抑制”指对植物中光合作用生产的光诱导减少。光抑制可通过本领域中任何已知的方法(例如,光饱和的氧释放的速率、叶绿体荧光的可变水平与最大水平的比率)测量。在一些实施方式中,bestrophin多肽定位于植物细胞的至少一种叶绿体的叶绿体被膜或叶绿体类囊体膜。在一些实施方式中,植物细胞是叶片叶肉细胞。在一些实施方式中,多肽在植物的至少70%的叶片叶肉细胞中表达。
在任何以上实施方式的一些实施方式中,修饰的核酸序列稳定地整合入植物的细胞核基因组中。在任何以上实施方式的一些实施方式中,至少一种修饰的核酸序列另外地含有可操作地连接至绿藻的bestrophin多肽的至少一种编码序列的、编码信号肽序列或靶向序列的第二核酸序列,其中信号肽序列或靶向序列的表达导致bestrophin多肽定位于植物细胞的至少一种叶绿体的叶绿体被膜或叶绿体类囊体膜。在一些实施方式中,信号肽序列或靶向序列是前导序列。在一些实施方式中,信号肽序列或靶向序列是本领域已知的导致在叶绿体被膜或叶绿体类囊体膜中多肽表达的任何序列。
在任何以上实施方式的一些实施方式中,增加的碳利用效率、增加的水利用效率、增加的氮利用效率或减少的光抑制是与SEQ ID NO:1具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%或至少95%序列同一性的第一多肽,与SEQID NO:2具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%或至少95%序列同一性的第二多肽,与SEQ ID NO:3具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%或至少95%序列同一性的第三多肽或其任何组合的表达的结果。在任何以上实施方式的一些实施方式中,增加的碳利用效率、增加的水利用效率、增加的氮利用效率或减少的光抑制是与SEQ ID NO:1具有至少95%序列同一性的第一多肽、与SEQ ID NO:2具有至少95%序列同一性的第二多肽、与SEQ ID NO:3具有至少95%序列同一性的第三多肽或其任何组合的表达的结果。
在任何以上实施方式的一些实施方式中,碳酸氢盐跨过膜的能力的增加或提供是来自组SEQ ID NO:4、SEQ ID NO:5、SEQ ID NO:6、SEQ ID NO:7、SEQ ID NO:8、SEQ ID NO:9、SEQ ID NO:10、SEQ ID NO:11、SEQ ID NO:12、SEQ ID NO:13、SEQ ID NO:14、SEQ ID NO:15、SEQ ID NO:16、SEQ ID NO:17、SEQ ID NO:18、SEQ ID NO:19、SEQ ID NO:20、SEQ IDNO:21、SEQ ID NO:22、SEQ ID NO:23、SEQ ID NO:24、SEQ ID NO:25、SEQ ID NO:26、SEQ IDNO:27、SEQ ID NO:28、SEQ ID NO:29、SEQ ID NO:30、SEQ ID NO:31、SEQ ID NO:32、SEQ IDNO:33、SEQ ID NO:34、SEQ ID NO:35、SEQ ID NO:36、SEQ ID NO:37、SEQ ID NO:38、SEQ IDNO:39、SEQ ID NO:40、SEQ ID NO:41、SEQ ID NO:42、SEQ ID NO:43、SEQ ID NO:44、SEQ IDNO:45、SEQ ID NO:46、SEQ ID NO:47、SEQ ID NO:48、SEQ ID NO:49、SEQ ID NO:50、SEQ IDNO:51、SEQ ID NO:52、SEQ ID NO:53、SEQ ID NO:54、SEQ ID NO:55、SEQ ID NO:56、SEQ IDNO:57、SEQ ID NO:58、SEQ ID NO:59、SEQ ID NO:60、SEQ ID NO:61、SEQ ID NO:62、SEQ IDNO:63、SEQ ID NO:64、SEQ ID NO:65、SEQ ID NO:66、SEQ ID NO:67、SEQ ID NO:68、SEQ IDNO:69、SEQ ID NO:70、SEQ ID NO:71、SEQ ID NO:72、SEQ ID NO:73、SEQ ID NO:74、SEQ IDNO:75、SEQ ID NO:76、SEQ ID NO:77、SEQ ID NO:78、SEQ ID NO:79、SEQ ID NO:80、SEQ IDNO:81、SEQ ID NO:82、SEQ ID NO:83、SEQ ID NO:84、SEQ ID NO:85、SEQ ID NO:86、SEQ IDNO:87、SEQ ID NO:88、SEQ ID NO:89、SEQ ID NO:90、SEQ ID NO:91、SEQ ID NO:92、SEQ IDNO:93、SEQ ID NO:94、SEQ ID NO:95、SEQ ID NO:96、SEQ ID NO:97、SEQ ID NO:98、SEQ IDNO:99、SEQ ID NO:100、SEQ ID NO:101、SEQ ID NO:102、SEQ ID NO:103、SEQ ID NO:104、SEQ ID NO:105、SEQ ID NO:106、SEQ ID NO:107、SEQ ID NO:108、SEQ ID NO:109、SEQ IDNO:110或SEQ ID NO:111,优选地选自由SEQ ID NO:10、SEQ ID NO:11、SEQ ID NO:15、SEQID NO:27、SEQ ID NO:28、SEQ ID NO:29、SEQ ID NO:42、SEQ ID NO:62或SEQ ID NO:63组成的组中的多肽的表达的结果。
在任何以上实施方式的一些实施方式中,植物是豇豆(即,黑眼豌豆、豇豆(Vignaunguiculata)),大豆(即,黄豆、大豆(Glycine max)),木薯(即,树薯、木薯(Manihotesculenta)),水稻(即,稻(Oryza sativa)、非洲栽培稻(Oryza glaberrima)、菰属物种(Zizania spp.)),小麦(即,普通小麦、斯佩耳特小麦,硬质小麦、小麦(Triticumaestivum)、斯佩耳特小麦(Triticum spelta)、硬质小麦(Triticum durum)、小麦属物种(Triticum spp.)),大麦(即,栽培大麦(Hordeum vulgare)),黑麦(即,黑麦(Secalecereale)),燕麦(即,燕麦(Avena sativa)),土豆(即,马铃薯(Solanum tuberosum)),西红柿(即,番茄(Solanum lycopersicum))或另一C3作物植物。在一些实施方式中,植物是烟草(即,普通烟草(Nicotiana tabacum)、Nicotiana edwardsonii、Nicotianaplumbagnifolia、Nicotiana longiflora)或拟南芥属(即,筷子芥,阿拉伯芥、拟南芥(Arabidopsis thaliana))。在任何以上实施方式的一些实施方式中,植物不是玉米(即,玉蜀黍、玉米(Zea mays)),高粱(即,durra、大谷子、milo、高粱(Sorghum bicolor)),甘蔗(即,甘蔗(sugar cane)、甘蔗(Saccharum officinarum)),小米(即,龙爪稷、普通小米、珍珠稷、栗、穇子(Eleusine coracana)、稷(Panicum miliaceum)、珍珠粟(Pennisetumglaucum)、粟(Setaria italica)),柳枝稷(即,高恐慌草、茅草、Panicum virganum)或另一C4作物植物。
在一些实施方式中,任何以上实施方式的植物部分是叶、茎、根、花、种子、果实、细胞或其部分。在一些实施方式中,植物部分是果实。在一些实施方式中,植物部分是籽粒、果仁、豆或块茎。
在一些方面中,本公开涉及任何以上实施方式的花粉粒或胚珠。
在一些方面中,本公开涉及由任何以上实施方式产生的原生质体。
在一些方面中,本公开涉及由任何以上实施方式的原生质体或细胞产生的组织培养物,其中细胞或原生质体由叶、花药、雌蕊、茎、叶柄、根、根尖、果实、种子、花、子叶、下胚轴、胚胎或分生组织细胞的组中的植物部分之一产生。
在一些方面中,本公开涉及含有一个或多个基因改变的基因改变的种子,所述基因改变增加或提供碳酸氢盐跨过膜的能力。在一些实施方式中,种子产生具有碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力的植物。在一些实施方式中,种子产生具有碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力的植物。在一些实施方式中,植物表达至少一种绿藻的bestrophin多肽。在一些实施方式中,绿藻的bestrophin多肽是SEQ ID NO:1、SEQ ID NO:2、SEQ ID NO:3或其任何组合。在一些实施方式中,植物表达与SEQ ID NO:1具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%或至少95%序列同一性的第一多肽,与SEQ ID NO:2具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%或至少95%序列同一性的第二多肽,与SEQ ID NO:3具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%或至少95%序列同一性的第三多肽或其任何组合。在一些实施方式中,植物表达与SEQ ID NO:1具有至少95%序列同一性的第一多肽、与SEQ IDNO:2具有至少95%序列同一性的第二多肽、与SEQ ID NO:3具有至少95%序列同一性的第三多肽或其任何组合。在一些实施方式中,植物表达来自组SEQ ID NO:4、SEQ ID NO:5、SEQID NO:6、SEQ ID NO:7、SEQ ID NO:8、SEQ ID NO:9、SEQ ID NO:10、SEQ ID NO:11、SEQ IDNO:12、SEQ ID NO:13、SEQ ID NO:14、SEQ ID NO:15、SEQ ID NO:16、SEQ ID NO:17、SEQ IDNO:18、SEQ ID NO:19、SEQ ID NO:20、SEQ ID NO:21、SEQ ID NO:22、SEQ ID NO:23、SEQ IDNO:24、SEQ ID NO:25、SEQ ID NO:26、SEQ ID NO:27、SEQ ID NO:28、SEQ ID NO:29、SEQ IDNO:30、SEQ ID NO:31、SEQ ID NO:32、SEQ ID NO:33、SEQ ID NO:34、SEQ ID NO:35、SEQ IDNO:36、SEQ ID NO:37、SEQ ID NO:38、SEQ ID NO:39、SEQ ID NO:40、SEQ ID NO:41、SEQ IDNO:42、SEQ ID NO:43、SEQ ID NO:44、SEQ ID NO:45、SEQ ID NO:46、SEQ ID NO:47、SEQ IDNO:48、SEQ ID NO:49、SEQ ID NO:50、SEQ ID NO:51、SEQ ID NO:52、SEQ ID NO:53、SEQ IDNO:54、SEQ ID NO:55、SEQ ID NO:56、SEQ ID NO:57、SEQ ID NO:58、SEQ ID NO:59、SEQ IDNO:60、SEQ ID NO:61、SEQ ID NO:62、SEQ ID NO:63、SEQ ID NO:64、SEQ ID NO:65、SEQ IDNO:66、SEQ ID NO:67、SEQ ID NO:68、SEQ ID NO:69、SEQ ID NO:70、SEQ ID NO:71、SEQ IDNO:72、SEQ ID NO:73、SEQ ID NO:74、SEQ ID NO:75、SEQ ID NO:76、SEQ ID NO:77、SEQ IDNO:78、SEQ ID NO:79、SEQ ID NO:80、SEQ ID NO:81、SEQ ID NO:82、SEQ ID NO:83、SEQ IDNO:84、SEQ ID NO:85、SEQ ID NO:86、SEQ ID NO:87、SEQ ID NO:88、SEQ ID NO:89、SEQ IDNO:90、SEQ ID NO:91、SEQ ID NO:92、SEQ ID NO:93、SEQ ID NO:94、SEQ ID NO:95、SEQ IDNO:96、SEQ ID NO:97、SEQ ID NO:98、SEQ ID NO:99、SEQ ID NO:100、SEQ ID NO:101、SEQID NO:102、SEQ ID NO:103、SEQ ID NO:104、SEQ ID NO:105、SEQ ID NO:106、SEQ ID NO:107、SEQ ID NO:108、SEQ ID NO:109、SEQ ID NO:110或SEQ ID NO:111,优选地选自由SEQID NO:10、SEQ ID NO:11、SEQ ID NO:15、SEQ ID NO:27、SEQ ID NO:28、SEQ ID NO:29、SEQID NO:42、SEQ ID NO:62或SEQ ID NO:63组成的组中的多肽。在一些实施方式中,多肽定位于植物细胞的至少一种叶绿体的叶绿体被膜或叶绿体类囊体膜。在一些实施方式中,植物细胞是叶片叶肉细胞。在一些实施方式中,多肽在植物的至少70%的叶片叶肉细胞中表达。在任何以上实施方式的一些实施方式中,植物是豇豆(即,黑眼豌豆、豇豆(Vignaunguiculata)),大豆(即,黄豆、大豆(Glycine max)),木薯(即,树薯、木薯(Manihotesculenta)),水稻(即,稻(Oryza sativa)、非洲栽培稻(Oryza glaberrima)、菰属物种(Zizania spp.)),小麦(即,普通小麦、斯佩耳特小麦,硬质小麦、小麦(Triticumaestivum)、斯佩耳特小麦(Triticum spelta)、硬质小麦(Triticum durum)、小麦属物种(Triticum spp.)),大麦(即,栽培大麦(Hordeum vulgare)),黑麦(即,黑麦(Secalecereale)),燕麦(即,燕麦(Avena sativa)),土豆(即,马铃薯(Solanum tuberosum)),西红柿(即,番茄(Solanum lycopersicum))或另一C3作物植物。在一些实施方式中,植物是烟草(即,普通烟草(Nicotiana tabacum)、Nicotiana edwardsonii、Nicotianaplumbagnifolia、Nicotiana longiflora)或拟南芥属(即,筷子芥,阿拉伯芥、拟南芥(Arabidopsis thaliana))。在任何以上实施方式的一些实施方式中,植物不是玉米(即,玉蜀黍、玉米(Zea mays)),高粱(即,durra、大谷子、milo、高粱(Sorghum bicolor)),甘蔗(即,甘蔗(sugar cane)、甘蔗(Saccharum officinarum)),小米(即,龙爪稷、普通小米、珍珠稷、栗、穇子(Eleusine coracana)、稷(Panicum miliaceum)、珍珠粟(Pennisetumglaucum)、粟(Setaria italica)),柳枝稷(即,高恐慌草、茅草、Panicum virganum)或另一C4作物植物。
在任何以上实施方式的一些实施方式中,调节内源碳酸酐酶的表达。在一些实施方式中,调节的表达可以是增加的表达、减少的表达、在不同位置的表达或其任何组合。
产生和栽培基因改变的植物的方法
本公开的某些方面涉及产生具有增加的碳利用效率的植物的方法,其中方法的步骤是:a)将基因改变引入植物,导致增加或提供碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力或者增加或提供碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力,从而增加植物的碳利用效率。
在一些方面中,本公开涉及产生具有增加的水利用效率的植物的方法,其中方法的步骤是:a)将基因改变引入植物,导致增加或提供碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力或者增加或提供碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力,从而增加植物的水利用效率。
在一些方面中,本公开涉及产生具有增加的氮利用效率的植物的方法,其中方法的步骤是:a)将基因改变引入植物,导致增加或提供碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力或者增加或提供碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力,从而增加植物的氮利用效率。
在一些方面中,本公开涉及产生具有减少的光抑制的植物的方法,其中方法的步骤是:a)将基因改变引入植物,导致增加或提供碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力或者增加或提供碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力,从而减少植物的光抑制。
在一些方面中,本公开涉及产生具有增加的生长或生产率的植物的方法,其中方法的步骤是:a)将基因改变引入植物,导致增加或提供碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力或者增加或提供碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力,从而增加植物的生长或生产率。对增加的生长的测量可包括但不限于更快的生长速率、更大的植物、增加的生物量、增加的干质量、增加的芽质量和增加的根质量。对增加的产率的测量可包括但不限于更高的作物产率、更多的叶数量和到作物成熟更少的天数。
在任何以上方法的一些实施方式中,调节内源碳酸酐酶的表达。在一些实施方式中,调节的表达可以是增加的表达、减少的表达、在不同位置的表达或其任何组合。
在一些方面中,本公开涉及栽培具有增加的碳利用效率的植物的方法,其中方法的步骤是:a)提供具有一个或多个基因改变的种子,所述基因改变增加或提供碳酸氢盐跨过膜的能力,其中种子产生具有碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力的植物或者具有碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力的植物;b)在一定条件下栽培植物,其中与在相同条件下生长的缺少一个或多个基因改变的植物相比,碳酸氢盐跨过膜的能力增加碳利用效率。
在一些方面中,本公开涉及栽培具有增加的水利用效率的植物的方法,其中方法的步骤是:a)提供具有一个或多个基因改变的种子,所述基因改变增加或提供碳酸氢盐跨过膜的能力,其中种子产生具有碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力的植物或者具有碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力的植物;b)在一定条件下栽培植物,其中与在相同条件下生长的缺少一个或多个基因改变的植物相比,碳酸氢盐跨过膜的能力增加水利用效率。
在一些方面中,本公开涉及栽培具有增加的氮利用效率的植物的方法,其中方法的步骤是:a)提供具有一个或多个基因改变的种子,所述基因改变增加或提供碳酸氢盐跨过膜的能力,其中种子产生具有碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力的植物或者具有碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力的植物;b)在一定条件下栽培植物,其中与在相同条件下生长的缺少一个或多个基因改变的植物相比,碳酸氢盐跨过膜的能力增加氮利用效率。
在一些方面中,本公开涉及栽培具有减少的光抑制的植物的方法,其中方法的步骤是:a)提供具有一个或多个基因改变的种子,所述基因改变增加或提供碳酸氢盐跨过膜的能力,其中种子产生具有碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力的植物或者具有碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力的植物;b)在一定条件下栽培植物,其中与在相同条件下生长的缺少一个或多个基因改变的植物相比,碳酸氢盐跨过膜的能力减少光抑制。
在一些方面中,本公开涉及栽培具有增加的生长或生产率的植物的方法,其中方法的步骤是:a)提供具有一个或多个基因改变的种子,所述基因改变增加或提供碳酸氢盐跨过膜的能力,其中种子产生具有碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力的植物或者具有碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力的植物;b)在一定条件下栽培植物,其中与在相同条件下生长的缺少一个或多个基因改变的植物相比,碳酸氢盐跨过膜的能力增加生长或生产率。
在一些方面中,本公开涉及栽培具有增加的碳利用效率的植物的方法,其中方法的步骤是:a)提供具有一个或多个基因改变的组织培养物或原生质体,所述基因改变增加或提供碳酸氢盐跨过膜的能力;b)使组织培养物或原生质体再生为小植株;c)使小植株生长为植物,其中植物具有碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力或者碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力;d)将植物移植到一定条件中,其中与在相同条件下生长的缺少一个或多个基因改变的植物相比,碳酸氢盐跨过膜的能力增加碳利用效率。
在一些方面中,本公开涉及栽培具有增加的水利用效率的植物的方法,其中方法的步骤是:a)提供具有一个或多个基因改变的组织培养物或原生质体,所述基因改变增加或提供碳酸氢盐跨过膜的能力;b)使组织培养物或原生质体再生为小植株;c)使小植株生长为植物,其中植物具有碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力或者碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力;d)将植物移植到一定条件中,其中与在相同条件下生长的缺少一个或多个基因改变的植物相比,碳酸氢盐跨过膜的能力增加水利用效率。
在一些方面中,本公开涉及栽培具有增加的氮利用效率的植物的方法,其中方法的步骤是:a)提供具有一个或多个基因改变的组织培养物或原生质体,所述基因改变增加或提供碳酸氢盐跨过膜的能力;b)使组织培养物或原生质体再生为小植株;c)使小植株生长为植物,其中植物具有碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力或者碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力;d)将植物移植到一定条件中,其中与在相同条件下生长的缺少一个或多个基因改变的植物相比,碳酸氢盐跨过膜的能力增加氮利用效率。
在一些方面中,本公开涉及栽培具有减少的光抑制的植物的方法,其中方法的步骤是:a)提供具有一个或多个基因改变的组织培养物或原生质体,所述基因改变增加或提供碳酸氢盐跨过膜的能力;b)使组织培养物或原生质体再生为小植株;c)使小植株生长为植物,其中植物具有碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力或者碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力;d)将植物移植到一定条件中,其中与在相同条件下生长的缺少一个或多个基因改变的植物相比,碳酸氢盐跨过膜的能力减少光抑制。
在一些方面中,本公开涉及栽培具有增加的生长或生产率的植物的方法,其中方法的步骤是:a)提供具有一个或多个基因改变的组织培养物或原生质体,所述基因改变增加或提供碳酸氢盐跨过膜的能力;b)使组织培养物或原生质体再生为小植株;c)使小植株生长为植物,其中植物具有碳酸氢盐跨过膜从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力或者碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力;d)将植物移植到一定条件中,其中与在相同条件下生长的缺少一个或多个基因改变的植物相比,碳酸氢盐跨过膜的能力增加生长或生产率。
在任何以上方法的一些实施方式中,种子、组织培养物或原生质体具有调节内源碳酸酐酶的表达的一个或多个基因改变。在一些实施方式中,调节的表达可以是增加的表达、减少的表达、在不同位置的表达或其任何组合。
在任何以上方法的一些实施方式中,碳酸氢盐跨过膜的能力的增加或提供是选自与SEQ ID NO:1具有至少95%序列同一性的第一多肽、与SEQ ID NO:2具有至少95%序列同一性的第二多肽、与SEQ ID NO:3具有至少95%序列同一性的第三多肽或其任何组合的组中的至少一种多肽的表达的结果。任何以上方法的,碳酸氢盐跨过膜的能力的增加或提供是选自SEQ ID NO:4、SEQ ID NO:5、SEQ ID NO:6、SEQ ID NO:7、SEQ ID NO:8、SEQ ID NO:9、SEQ ID NO:10、SEQ ID NO:11、SEQ ID NO:12、SEQ ID NO:13、SEQ ID NO:14、SEQ ID NO:15、SEQ ID NO:16、SEQ ID NO:17、SEQ ID NO:18、SEQ ID NO:19、SEQ ID NO:20、SEQ IDNO:21、SEQ ID NO:22、SEQ ID NO:23、SEQ ID NO:24、SEQ ID NO:25、SEQ ID NO:26、SEQ IDNO:27、SEQ ID NO:28、SEQ ID NO:29、SEQ ID NO:30、SEQ ID NO:31、SEQ ID NO:32、SEQ IDNO:33、SEQ ID NO:34、SEQ ID NO:35、SEQ ID NO:36、SEQ ID NO:37、SEQ ID NO:38、SEQ IDNO:39、SEQ ID NO:40、SEQ ID NO:41、SEQ ID NO:42、SEQ ID NO:43、SEQ ID NO:44、SEQ IDNO:45、SEQ ID NO:46、SEQ ID NO:47、SEQ ID NO:48、SEQ ID NO:49、SEQ ID NO:50、SEQ IDNO:51、SEQ ID NO:52、SEQ ID NO:53、SEQ ID NO:54、SEQ ID NO:55、SEQ ID NO:56、SEQ IDNO:57、SEQ ID NO:58、SEQ ID NO:59、SEQ ID NO:60、SEQ ID NO:61、SEQ ID NO:62、SEQ IDNO:63、SEQ ID NO:64、SEQ ID NO:65、SEQ ID NO:66、SEQ ID NO:67、SEQ ID NO:68、SEQ IDNO:69、SEQ ID NO:70、SEQ ID NO:71、SEQ ID NO:72、SEQ ID NO:73、SEQ ID NO:74、SEQ IDNO:75、SEQ ID NO:76、SEQ ID NO:77、SEQ ID NO:78、SEQ ID NO:79、SEQ ID NO:80、SEQ IDNO:81、SEQ ID NO:82、SEQ ID NO:83、SEQ ID NO:84、SEQ ID NO:85、SEQ ID NO:86、SEQ IDNO:87、SEQ ID NO:88、SEQ ID NO:89、SEQ ID NO:90、SEQ ID NO:91、SEQ ID NO:92、SEQ IDNO:93、SEQ ID NO:94、SEQ ID NO:95、SEQ ID NO:96、SEQ ID NO:97、SEQ ID NO:98、SEQ IDNO:99、SEQ ID NO:100、SEQ ID NO:101、SEQ ID NO:102、SEQ ID NO:103、SEQ ID NO:104、SEQ ID NO:105、SEQ ID NO:106、SEQ ID NO:107、SEQ ID NO:108、SEQ ID NO:109、SEQ IDNO:110或SEQ ID NO:111的组,优选地选自由SEQ ID NO:10、SEQ ID NO:11、SEQ ID NO:15、SEQ ID NO:27、SEQ ID NO:28、SEQ ID NO:29、SEQ ID NO:42、SEQ ID NO:62或SEQ ID NO:63组成的组中的至少一种多肽的表达的结果。在一些实施方式中,多肽定位于植物细胞的至少一种叶绿体的叶绿体被膜或叶绿体类囊体膜。在一些实施方式中,植物细胞是叶片叶肉细胞。在一些实施方式中,多肽在植物的至少70%的叶片叶肉细胞中表达。在任何以上实施方式的一些实施方式中,植物是豇豆(即,黑眼豌豆、豇豆(Vigna unguiculata)),大豆(即,黄豆、大豆(Glycine max)),木薯(即,树薯、木薯(Manihot esculenta)),水稻(即,稻(Oryza sativa)、非洲栽培稻(Oryza glaberrima)、菰属物种(Zizania spp.)),小麦(即,普通小麦、斯佩耳特小麦,硬质小麦、小麦(Triticum aestivum)、斯佩耳特小麦(Triticumspelta)、硬质小麦(Triticum durum)、小麦属物种(Triticum spp.)),大麦(即,栽培大麦(Hordeum vulgare)),黑麦(即,黑麦(Secale cereale)),燕麦(即,燕麦(Avena sativa)),土豆(即,马铃薯(Solanum tuberosum)),西红柿(即,番茄(Solanum lycopersicum))或另一C3作物植物。在一些实施方式中,植物是烟草(即,普通烟草(Nicotiana tabacum)、Nicotiana edwardsonii、Nicotiana plumbagnifolia、Nicotiana longiflora)或拟南芥属(即,筷子芥,阿拉伯芥、拟南芥(Arabidopsis thaliana))。在任何以上实施方式的一些实施方式中,植物不是玉米(即,玉蜀黍、玉米(Zea mays)),高粱(即,durra、大谷子、milo、高粱(Sorghum bicolor)),甘蔗(即,甘蔗(sugar cane)、甘蔗(Saccharum officinarum)),小米(即,龙爪稷、普通小米、珍珠稷、栗、穇子(Eleusine coracana)、稷(Panicummiliaceum)、珍珠粟(Pennisetum glaucum)、粟(Setaria italica)),柳枝稷(即,高恐慌草、茅草、Panicum virganum)或另一C4作物植物。
基因改变的海藻
本公开的某些方面涉及含有一个或多个基因改变的基因改变的藻类,所述基因改变导致增加藻类将碳酸氢盐转运进入藻类的叶绿体的腔的能力。在一些实施方式中,碳酸氢盐转运能力的增加是过表达选自与SEQ ID NO:1具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%或至少95%序列同一性的第一多肽,与SEQID NO:2具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%或至少95%序列同一性的第二多肽,与SEQ ID NO:3具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%或至少95%序列同一性的第三多肽或其任何组合的组中的至少一种绿藻的bestrophin多肽的结果。在一些实施方式中,碳酸氢盐转运能力的增加是过表达选自与SEQ ID NO:1具有至少95%序列同一性的第一多肽、与SEQ ID NO:2具有至少95%序列同一性的第二多肽、与SEQ ID NO:3具有至少95%序列同一性的第三多肽或其任何组合的组中的至少一种绿藻的bestrophin多肽的结果。在一些实施方式中,多肽定位于叶绿体类囊体膜。在一些实施方式中,至少当藻类在<100ppm二氧化碳(空气中<0.01%[v/v]CO2)条件下时,过表达多肽。
在一些方面中,本公开涉及具有增加的碳酸氢盐转运的绿藻或其部分,其含有包含至少一种绿藻的bestrophin多肽的编码序列的修饰的核酸序列的;其中过表达bestrophin多肽;其中bestrophin多肽定位于叶绿体类囊体膜;并且其中当在<100ppm二氧化碳(空气中<0.01%[v/v]CO2)条件下栽培藻类时,产率、生长速率或生物量大于来自不过表达至少一种bestrophin多肽的相应的野生型(WT)藻类或其WT部分的产率、生长速率或生物量或者产率、生长速率或生物量基本上类似于来自在<100ppm二氧化碳(空气中<0.01%[v/v]CO2)条件下栽培的不过表达至少一种bestrophin多肽的相应的WT藻类或相应的其WT部分的产率、生长速率或生物量。在一些实施方式中,至少一种绿藻的bestrophin多肽选自与SEQ ID NO:1具有至少95%序列同一性的第一多肽、与SEQ ID NO:2具有至少95%序列同一性的第二多肽、与SEQ ID NO:3具有至少95%序列同一性的第三多肽或其任何组合的组。
在一些方面中,本公开涉及在<100ppm二氧化碳(<0.01%[v/v]CO2in空气)条件下具有增加的生长的绿藻或其部分,其含有包含至少一种绿藻的bestrophin多肽的编码序列的修饰的核酸序列;其中过表达bestrophin多肽;其中bestrophin多肽定位于叶绿体类囊体膜;并且其中当在<100ppm二氧化碳(空气中<0.01%[v/v]CO2)条件下栽培藻类时,产率、生长速率或生物量大于来自不过表达至少一种bestrophin多肽的相应的野生型(WT)藻类或其WT部分的产率、生长速率或生物量或者产率、生长速率或生物量基本上类似于来自在<100ppm二氧化碳(空气中<0.01%[v/v]CO2)条件下栽培的不过表达至少一种bestrophin多肽的相应的WT藻类或相应的其WT部分的产率、生长速率或生物量。在一些实施方式中,至少一种绿藻的bestrophin多肽选自与SEQ ID NO:1具有至少95%序列同一性的第一多肽、与SEQ ID NO:2具有至少95%序列同一性的第二多肽、与SEQ ID NO:3具有至少95%序列同一性的第三多肽或其任何组合的组。
在任何以上藻类的一些实施方式中,绿藻选自莱茵衣藻、Chlamydomonaseustigma、Volvox carteri f.nagariensis和胸状盘藻的组中。
产生基因改变的藻类的方法
在一些方面中,本公开涉及产生具有增加的碳利用效率的藻类的方法,其中方法的步骤是:a)将基因改变引入藻类,包括增加将碳酸氢盐转运进入藻类的叶绿体的腔的能力,从而增加藻类的碳利用效率。在一些实施方式中,转运能力的获得是过表达选自与SEQID NO:1具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%或至少95%序列同一性的第一多肽,与SEQ ID NO:2具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%或至少95%序列同一性的第二多肽,与SEQ ID NO:3具有至少70%序列同一性,至少71%、72%、73%、74%、75%、76%、77%、78%、79%、80%、81%、82%、83%、84%、85%、86%、87%、88%、89%、90%、91%、92%、93%、94%或至少95%序列同一性的第三多肽或其任何组合的组中的至少一种绿藻的bestrophin多肽的结果。在一些实施方式中,碳酸氢盐转运能力的增加是过表达选自与SEQID NO:1具有至少95%序列同一性的第一多肽、与SEQ ID NO:2具有至少95%序列同一性的第二多肽、与SEQ ID NO:3具有至少95%序列同一性的第三多肽或其任何组合的组中的至少一种绿藻的bestrophin多肽的结果。
产生转基因植物和植物细胞的分子生物学方法
本发明的一个实施方式提供了包括一个或多个修饰的植物基因和/或引入基因的植物或植物细胞。例如,本公开提供了具有增加的或提供的碳酸氢盐跨过膜从植物细胞细胞质从植物细胞细胞质进入植物的至少一部分叶绿体的基质的能力的转基因植物。进一步,本公开提供了具有增加的或提供的碳酸氢盐跨过膜从基质进入植物的至少一部分叶绿体的腔的能力的转基因植物。另外,本公开提供了具有含有绿藻的bestrophin多肽的至少一种编码序列的至少一种修饰的核酸序列的转基因植物。本文也考虑和描述了对其他基因元件(例如,内源碳酸酐酶)的调节的表达。
基因改变的单子叶植物和双子叶植物植物细胞的转化和产生是本领域熟知的。参见,例如,Weising等,Ann.Rev.Genet.22:421-477(1988);美国专利5,679,558;Agrobacterium Protocols,ed:Gartland,Humana Press Inc.(1995)和Wang等,ActaHort.461:401-408(1998)。方法的选择随着待转化的植物的类型、特定的应用和/或期望的结果而改变。通过熟练的从业者容易选择适当的转化技术。
本领域已知的删除、插入或以其他修饰细胞DNA(例如,基因组DNA和细胞器DNA)的任何方法可用于实践本文公开的发明。例如,在根癌农杆菌(Agrobacterium tumefaciens)中含有删除或插入靶基因的基因构建体的丧失功能的Ti质粒可用于转化植物细胞,并且其后,可使用本领域描述的过程从转化的植物细胞再生转化的植物,例如,在EP 0116718、EP0270822、PCT公布WO 84/02913和公布的欧洲专利申请("EP")0242246中描述的过程。Ti-质粒载体各自含有Ti-质粒的T-DNA的边界序列之间,或至少定位于右侧边界序列的左侧的基因。当然,载体的其他类型可用于转化植物细胞,使用过程比如直接基因转移(例如在EP0233247中描述的)、花粉介导的转化(例如在EP 0270356、PCT公布WO 85/01856和美国专利4,684,611中描述的)、植物RNA病毒-介导的转化(例如在EP 0 067 553和美国专利4,407,956中描述的)、脂质体-介导的转化(例如在美国专利4,536,475中描述的)和其他方法比如用于转化玉米(例如,美国专利6,140,553;Fromm等,Bio/Technology(1990)8,833 839);Gordon-Kamm等,The Plant Cell,(1990)2,603 618)和水稻(Shimamoto等,Nature,(1989)338,274 276;Datta等,Bio/Technology,(1990)8,736 740)的某些品系的方法和一般用于转化单子叶植物的方法(PCT公布WO 92/09696)。对于棉花转化,可使用PCT专利公布WO 00/71733中描述的方法。对于大豆转化,参考本领域已知的方法,例如,Hinchee等(Bio/Technology,(1988)6,915)和Christou等(Trends Biotech,(1990)8,145)或WO 00/42207的方法。
本发明的转基因植物可用于常规的植物培育方案以产生更多具有相同特征的转基因植物,或在相同或相关的植物物种的其他品种中引入基因改变。从转化植物获得的种子优选地含有基因改变作为染色体或细胞器DNA中的稳定的插入物。根据本发明的包括基因改变的植物包括或衍生自包括本发明的基因改变的植物的根茎的植物,例如果树或观赏植物。因此,在转化的植物或植物部分上插入的任何非转基因嫁接的植物部分包括在本发明中。
导致引入的基因的表达的引入的基因元件无论是否在表达载体或表达盒中将通常利用植物可表达启动子。如本文使用的‘植物可表达启动子’指确保植物细胞中本发明的基因改变的表达的启动子。指导植物中组成型表达的启动子的示例是本领域已知的并且其包括:黄瓜花叶病毒(CaMV)的,例如,分离株CM 1841(Gardner等,Nucleic Acids Res,(1981)9,2871 2887)、CabbB S(Franck等,Cell(1980)21,285 294)和CabbB JI(Hull和Howell,Virology,(1987)86,482 493)的强组成型35S启动子("35S启动子");来自泛素家族的启动子(例如,Christensen等,Plant Mol Biol,(1992)18,675-689的玉米泛素启动子),gos2启动子(de Pater等,The Plant J(1992)2,834-844),emu启动子(Last等,TheorAppl Genet,(1990)81,581-588),肌动蛋白启动子比如通过An等(The Plant J,(1996)10,107)描述的启动子,通过Zhang等(The Plant Cell,(1991)3,1155-1165)描述的水稻肌动蛋白启动子;木薯静脉花叶病毒的启动子(WO 97/48819,Verdaguer等(Plant Mol Biol,(1998)37,1055-1067),来自地三叶草矮化病毒的pPLEX系列的启动子(WO 96/06932,特别地S4或S7启动子),醇脱氢酶启动子,例如,pAdh1S(GenBank登录号X04049、X00581)以及分别驱动T DNA的1'和2'基因的表达的TR1'启动子和TR2'启动子(分别为"TR1'启动子"和"TR2'启动子")(Velten等,EMBO J,(1984)3,2723 2730)。
备选地,植物可表达启动子可为组织特异性启动子,即在植物的一些细胞或组织中,例如,绿色组织中的指导较高水平的表达的启动子(比如PEP羧化酶的启动子)。已经描述植物PEP羧化酶启动子(Pathirana等,Plant J,(1997)12:293-304)为用于在脉管组织中表达的强启动子并且其用于本发明的一个实施方式中。备选地,植物可表达启动子也可为创伤诱导型启动子,比如豌豆细胞壁转化酶基因的启动子(Zhang等,Plant Physiol,(1996)112:1111-1117)。如本文使用的‘创伤诱导型’启动子意思是当机械地或通过昆虫进食使植物受伤时,在启动子控制下的编码序列的表达在该植物中得到显著增加。这些植物可表达启动子可与增强子元件组合,它们可与最小的启动子元件组合,或可包括重复元件以确保所需的表达谱。
在一些实施方式中,可利用增加在植物细胞中的表达的基因元件。例如,在引入的基因的5’端或3’端处,或在引入的基因的编码序列中的内含子,例如,hsp70内含子。其他这种基因元件可包括但不限于启动子增强子元件、二次重复的或三次重复的启动子区域、与另一转基因不同的或与内源(植物宿主)基因前导序列不同的5’前导序列、与相同植物中使用的另一转基因不同的或与内源(植物宿主)拖尾序列不同的3’拖尾序列。
本发明的引入的基因可插入宿主细胞DNA中使得插入的基因部分在合适的3'端转录调节信号(即,转录物形成和多腺苷酸化信号)的上游(即,5')。这优选地通过在植物细胞基因组(细胞核或叶绿体)中插入基因完成。优选的多腺苷酸化和转录物形成信号包括胭脂碱合酶基因(Depicker等,J.Molec Appl Gen,(1982)1,561-573)、章鱼碱合酶基因(Gielen等,EMBO J,(1984)3:835 845)、SCSV或苹果酸酶终止子(Schunmann et al.,Plant FunctBiol,(2003)30:453-460)和T DNA基因7(Velten and Schell,Nucleic Acids Res,(1985)13,6981 6998)的那些,其充当转化的植物细胞中的3'非翻译DNA序列。在一些实施方式中,一个或多个引入的基因稳定地整合入细胞核基因组。当核酸序列保持整合入细胞核基因组中并且在贯穿随后的植物世代中继续表达(即,产生可检测的mRNA转录物或蛋白质)时,存在稳定的整合。稳定的整合入细胞核基因组可通过任何本领域已知的方法(例如,微粒轰击、农杆菌介导的转化、CRISPR/Cas9、原生质体的电穿孔、微注射等)完成。
术语重组体或修饰的核酸指通过组合序列的两个其他分开的区段而制造的多核苷酸,这是通过基因工程技术或通过化学合成对分离的多核苷酸的区段的人工操作完成的。这样做可将所需功能的多核苷酸区段连接一起以产生所需功能的组合。
如本文使用的,术语“过表达”和“上调”指由于基因修饰,相对于野生型生物体(例如,植物、藻类)中的表达增加的表达(例如,mRNA、多肽等的)。在一些实施方式中,表达中的增加是大于野生型中的表达约10%的微小增加。在一些实施方式中,表达中的增加是相对于野生型中的表达50%或更多(例如,60%、70%、80%、100%等)的增加。在一些实施方式中,过表达内源基因。在一些实施方式中,外源基因由于被表达而过表达。植物或藻类中基因的过表达可通过任何本领域已知的方法实现,所述本领域已知的方法包括但不限于使用组成型启动子、诱导型启动子、高表达启动子(例如,PsaD启动子)、增强子、转录和/或翻译的调节序列、密码子优化、修饰的转录因子和/或控制待过表达的基因的表达的突变体或修饰的基因。
在重组的核酸用于表达、克隆或复制特定序列的地方,用于引入宿主细胞中而制备的DNA构建体将通常包括由宿主识别的复制系统(即载体),其包括编码所需多肽的预期的DNA片段,并且也可包括可操作地连接至多肽-编码区段的转录和翻译启动调节序列。另外,这种构建体可包括细胞定位信号(例如,叶绿体定位信号)。在优选的实施方式中,将这种DNA构建体引入到宿主细胞的基因组DNA、叶绿体DNA或线粒体DNA中。
在一些实施方式中,非整合的表达系统可用于诱导一个或多个引入的基因的表达。表达系统(表达载体)可包括,例如,复制或自主复制序列(ARS)和表达控制序列的起点、启动子、增强子和必要的处理信息位点比如核糖体-结合位点、RNA剪接位点、多腺苷酸化位点、转录终止子序列和mRNA稳定序列。信号肽也可包括在与相同或相关的物种的分泌多肽合适的地方中,其允许蛋白质跨过和/或嵌入细胞膜、细胞壁或从细胞分泌。
用于实践本文公开的发明的方法的可选择标志物可为阳性可选择标志物。典型地,阳性选择指其中只有目标重组体多核苷酸存在于细胞内时,基因改变的细胞可在有毒的物质存在下存活的情况。阴性可选择标志物和可筛选标志物也是本领域熟知的和是本发明考虑的。本领域技术人员将认识到任何可获得的相关标志物可用于实践本文公开的发明。
本发明的重组菌的筛选和分子分析可利用核酸杂交技术进行。杂交程序可用于鉴定多核苷酸,比如使用本文描述的技术修饰的那些,与本文教导的有用的对象调节序列具有足够的同源性。特定的杂交技术对本发明是不重要的。随着杂交技术的改善,都可由本领域技术人员容易地应用。可用对本领域技术人员已知的任何合适的标记对杂交探针进行标记。可改变杂交条件和洗涤条件,例如温度和盐浓度以改变检测阈值的严谨度。参见,例如,Sambrook等(1989)参阅下文或Ausubel等(1995)Current Protocols in MolecularBiology,John Wiley&Sons,NY,N.Y.,对杂交条件的进一步指导。
另外,对基因改变的菌株的筛选和分子分析以及所需分离的核酸的建立可使用聚合酶链式反应(PCR)进行。PCR是对核酸序列的重复的、酶促的、引发的合成。该过程是本领域技术人员熟知的并且通常使用的(参见Mullis,美国专利号4,683,195、4,683,202和4,800,159;Saiki等(1985)Science 230:1350-1354)。PCR基于对目标DNA片段的酶扩增,目标DNA片段的两侧是与靶序列的相对链杂交的两个寡核苷酸引物。引物以指向彼此的3’端定向。模板的热变性、引物与它们互补的序列的退火和用DNA聚合酶对退火的引物的延伸的重复循环导致对由PCR引物的5’端限定的区段的扩增。因为每种引物的延伸产物可用作其他引物的模板,每个循环实质上使先前的循环中产生的DNA模板的量加倍。这导致特定的靶片段的指数积聚,在几个小时内多达几百万倍。通过使用热稳定的DNA聚合酶比如从嗜热性细菌水生栖热菌(Thermus aquaticus)分离的Taq聚合酶,扩增过程可以是完全自动的。可使用的其他酶是对本领域技术人员已知的。
本发明的核酸和蛋白质也可涵盖特别地公开的序列的同系物。同源性(例如,序列同一性)可为50%-100%。在一些情况下,这种同源性大于80%、大于85%、大于90%或大于95%。本领域技术人容易鉴定对于序列得任何预期使用需要的同源性或同一性的程度。使用本领域已知的算法,比如通过Karlin和Altschul(1990)Proc.Natl.Acad.Sci.USA 87:2264-2268公开的,如Karlin和Altschul(1993)Proc.Natl.Acad.Sci.USA 90:5873-5877中修饰的那些确定如本文使用的两个核酸的百分比序列同一性。这种算法被并入Altschul等(1990)J.Mol.Biol.215:402-410的NBLAST和XBLAST程序。BLAST核苷酸搜索用NBLAST程序进行,评分=100,字长=12,以获得具有所需百分比序列同一性的核苷酸序列。为了获得用于比较目的的空位比对,如Altschul等(1997)Nucl.Acids.Res.25:3389-3402中描述的使用Gapped BLAST。当利用BLAST和Gapped BLAST程序时,使用各自程序(NBLAST和XBLAST)的默认参数。参见www.ncbi.nih.gov。
优选的宿主细胞是植物或藻类细胞。在本上下文中,重组体宿主细胞是已经基因修饰以含有分离的核酸分子、含有宿主细胞中正常存在并且有功能的一个或多个删除的或以其他方式的非功能性基因或含有产生至少一种重组体蛋白质的一个或多个基因的那些。本发明的编码蛋白质的核酸可通过适合于特定类型的细胞的领域已知的任何方式引入,其包括但不限于,转化、脂质转染、电穿孔或本领域技术人员已知的任何其他方法。
已经一般性地描述了本发明,通过参考某些具体的实施例将更好地理解本发明,这些实施例包括在本文中进一步阐释本发明并且不旨在如通过权利要求限定限制本发明的范围。
实施例
在以下实施例中进一步详细描述了本公开,所述实施例不以任何方式旨在限制所要求保护的本公开的范围。附图意味着被认为作为说明书的完整部分和本公开的描述。提供以下实施例以阐释,但是不限制所要求保护的公开内容。
实施例1:用于细胞培养和生长的一般材料和方法
以下实施例描述了在以下所有实施例中使用的细胞培养和生长条件。将莱茵衣藻细胞维持在陪替氏平皿上的磷酸三乙酸酯(TAP)培养基或乙酸酵母(YA)培养基上。实验之前,将细胞接种入基本培养基(即,不含有碳源)并且在高CO2(空气中>5%CO2)上生长至2-3x106个细胞mL-1的密度。然后,将这些细胞稀释入基本培养基并且在指示的pH和CO2浓度生长。
莱茵衣藻培养条件与Ma等,Plant Physiol 156:884-896,2011中描述的相同。从Dr.Rogene Schnell(University of Arkansas,Little Rock)获得D66菌株(nit2-、cw15、mt+)。在衣藻培养物保藏(Chlamydomonas culture collection)(Zhang等,Plant Cell 26(4):1398-1409,2014)处的CLiP保藏获得CMJ030(CC-4533;cw15,mt-)和bst3(BST3敲除LMJ.RY0402.089365)。根据Sueoka,PNAS 46:83-91,1960制备磷酸三乙酸酯(TAP)培养基、乙酸酵母(YA)培养基和基本培养基(MIN;不具有乙酸酯,即无碳源)。通过添加1.2%(w/v)琼脂制备TAP和YA陪替氏平皿二者,并且将莱茵衣藻细胞维持在两种类型的陪替氏平皿上。通过将来自TAP板的菌落接种入锥形烧瓶中的100mL TAP液体培养基,用于混合营养生长来启动细胞培养物。培养物在连续的照明(100μmol m-2s-1)下生长至早期的对数期并且震荡48小时。收获早期的对数期TAP-生长培养物并且用MIN培养基洗涤,然后在MIN培养基中再悬浮并且用高CO2(空气中5%[v/v]CO2)鼓泡以达到0.2和0.3(~2-3x106个细胞mL-1)之间的OD730。对于CCM诱导,将细胞转移至低CO2(空气中<0.01%[v/v]CO2)鼓泡12小时或环境CO2(空气中0.04%-0.045%[v/v]CO2)。
实施例2:bestrophin家族基因的系统进化树
以下实施例描述了描绘各种光合生物体中bestrophin家族基因的进化关系的系统进化树的构建。在哺乳动物中,已知bestrophin蛋白质传导氯化物和碳酸氢盐。在植物中,大多数bestrophin蛋白质还没有被表征。
材料和方法
为了多种原因,选择三种莱茵衣藻基因BESTROPHIN1(BST1)、BESTROPHIN2(BST2)和BESTROPHIN3(BST3)以用于该树。一个原因,BST1、BST2和BST3基因共享共同区域(参见图1A-1B)。另一个原因,BST1(SEQ ID NO:1)、BST2(SEQ ID NO:2)和BST3(SEQ ID NO:3)蛋白质的氨基酸序列彼此是>80%同一的(参见图1C)。莱茵衣藻含有七个其他预测的bestrophin,但是这些其他bestrophin没有一个与这三种蛋白质具有大于45%同一性。最后,预测所有选择的三种bestrophin靶向叶绿体。
系统进化树构建:BST1(Cre16.g662600.t1.2)、BST2(Cre16.g663400.t2.1)和BST3(Cre16.g663450.t1.2)的氨基酸序列是针对NCBI Genbank(Benson等,Nucleic AcidsRes,21(13):2963-2965,1993)和Phytozome v12.1(https://phytozome.jgi.doe.gov/pz/portal.html;Goodstein等,Nucleic Acids Res 40(Database issue):D1178-1186,2012)进行BLAST。下载使用这些数据库鉴定的精选(top hit)。另外,从NCBI Genbank下载编码智人BEST1(SJM31533.1)和肺炎克雷伯氏菌bestrophin(pdb_4WD8_A)的氨基酸序列以包括在内作为系统发生分析中的外群。使用ClustalW(Thompson等,Nucleic Acids Res 22(22):4673-4680,1994)算法与氨基酸置换矩阵BLOSUM62(Henikoff&Henikoff,PNAS 89(22):10915-10919,1992)在Geneious 11.1.4(Kearse等,Bioinformatics 28(12):1647-1649,2012)中对比总计63个初始序列。去除来自两个数据库(NCBI和Phytozome)的重复序列和具有小于70%的成对百分数正同一性(BLOSUM62)的序列。最终比对包括30个序列并且手动地调整以去除序列的可变长度端。在MEGA X(Kumar等,Comput Appl Biosci 10(2):189-191,1994)中完成系统发生分析。使用MEGA X中的模型选择函数计算比对的系统发生分析的最佳的最大似然(ML)模型。使用具有γ分布(5离散类别)和500辅助复制的LG置换模型(Le&Gascuel,Mol Biol Evol 25(7):1307-1320,2008)构建ML树(参见图1D)。AdobeIllustrator CC用于对树进行格式化和加阴影。使用最大似然(ML)构建莱茵衣藻BST1、BST2和BST3同系物的蛋白质序列的系统进化树。
结果
图1A示出BST1、BST2和BST3基因的示意图。浅灰色框表示外显子,细灰线表示内含子,粗灰线表示非翻译区(UTR),和重叠线(在外显子和内含子上重叠)指示由三种基因共享的共同区域。进一步,示出三种bestrophin基因的每个的长度,BST1为最短的并在3000kb以下和BST2为最长的并在5000kb以上。在图1B中,示出莱茵衣藻基因组上BST1、BST2和BST3基因的位置和定向。BST1(Cre16.g662600)、BST2(Cre16.g663400)和BST3(Cre16.g663450)是定位于莱茵衣藻的第16条染色体上130kbp区域内的旁系同源物基因。另外,该图像描绘了BST1、BST2和BST3相对于彼此的位置。图1C描绘了BST1、BST2和BST3蛋白质的氨基酸比对,其中底行中的星号指示在全部三个蛋白中相同的氨基酸。BST1、BST2和BST3蛋白质彼此是>80%同一的。
图1D显示表示各种光合生物体中莱茵衣藻BST1、BST2和BST3同系物的蛋白质序列中的关系的系统进化树。这些生物体包括维管植物(从顶部的第二组)、非维管植物(从顶部的第三组)、硅藻(从顶部的第四组)和绿藻(在底部的组)。莱茵衣藻bestrophin蛋白质在绿藻组内以粗体示出。也显示由来自非光合生物体人(智人)和细菌(肺炎克雷伯氏菌)的蛋白质组成的外群(在顶部的组)。莱茵衣藻BST1、BST2和BST3与人Bestrophin 1(BEST1)的序列比对示出BEST1和BST1-3之间的低序列同一性(21-23%)。莱茵衣藻bestrophin蛋白质彼此,并且与其他绿藻的bestrophin蛋白质显示出相当大的序列相似性,其通过它们在树内邻近的位置和连接这些蛋白质的更浅的分支模式进行描绘。具体地,团藻和Chlamydomonaseustigma与莱茵衣藻非常紧密地相关,而杜氏盐藻(Dunaliella salina)与莱茵衣藻是更远地相关。硅藻物种(以青色突出显示)与莱茵衣藻是非常远地相关,并且事实上由不同内共生事件引起;该远离的关系通过连接这些蛋白质的更深的分支模式清楚地描绘。在图1D中显示的维管和非维管植物蛋白质与莱茵衣藻bestrophin蛋白质具有仅30-35%的低序列相似性,其通过它们更远离的位置和连接这些蛋白质的更深的分支模式描绘。例如,拟南芥属的类囊体定位的VCCN1蛋白质与BST1-3具有约30%序列同一性。尽管用于构建该树的其他蛋白质也是bestrophin家族基因,但是它们与莱茵衣藻bestrophin仅是远地相关。在氨基酸水平,莱茵衣藻bestrophin与高等植物或哺乳动物中发现的bestrophin蛋白质具有小于50%同一性。进一步,人bestrophin蛋白质与莱茵衣藻bestrophin足够不同,使得其可用作外群,与细菌肺炎克雷伯杆菌bestrophin蛋白质一起,以锚定系统进化树。
实施例3:在低CO2下Bestrophin得到上调并且他们的表达被CIA5控制
CIA5是控制许多CCM基因的转录因子。具体地,CIA5控制所有已知的CCM转运蛋白。以下实施例描述了在高CO2或低CO2条件下生长的野生型(WT)D66莱茵衣藻菌株和cia5突变体莱茵衣藻菌株中bestrophin表达的分析。
材料和方法
用于莱茵衣藻细胞的细胞培养和生长条件如实施例1中描述的。对于实验,WT D66莱茵衣藻菌株和cia5突变体莱茵衣藻菌株在高CO2(空气中5%[v/v]CO2)或低CO2条件(空气中<0.04%[v/v]CO2,即环境CO2条件)下培养。
RNA样品从两种莱茵衣藻菌株的高CO2和低CO2适应的培养物获得。根据制造商的说明书使用Trizol试剂(Invitrogen)进行RNA提取。将每个样品的1ug RNA用作cDNA的模板,其根据制造商的说明书使用第一链cDNA合成试剂盒(NEB)制造的。将每个样品的100ng RNA用于进行qRTPCR,使用QuantStudio 6根据制造商的说明书使用来自NEB的通用一步骤RT-qPCR试剂盒。使用对BST1、BST2或BST3特异性的引物进行半定量的RT-PCR,并且将肌动蛋白引物作为对照(表1中列出)。使用凝胶电泳分析PCR产品。实验重复两次。
表1.用于RT-PCR分析的引物
还进行BST1、BST2和BST3表达的时间过程分析。对于该分析,将在高CO2下生长的细胞转移至低CO2放置2至12小时,然后提取RNA并且如以上所述的制造cDNA。该分析重复两次。
结果
如图2A中显示,在D66(WT)中,如与CO2条件相比,在低CO2条件下,所有三种bestrophin基因的表达水平得到上调。具体地,在高CO2条件下生长的细胞中,BST3表达非常低,并且在低CO2条件下生长的细胞中,BST3表达高得多。与在高CO2条件中生长的D66细胞相比,来自在低CO2条件中生长的D66细胞的条带的增加强度清楚地显示在低CO2条件下,所有三种基因得到上调。与D66形成对照,低CO2条件不诱导cia5突变体中BST1、BST2或BST3的表达增加。而且,BST1和BST3在任一条件下在cia5突变体中都不表达,其与在cia5突变体中其他CCM基因是类似的转录模式(Xiang等,PNAS 98(9):5341-5346,2001;Fukuzawa等,PNAS98(9):5347-5352,2001;Moroney等,Plant Physiol 89(3):897-903,1989)。cia5细胞中的BST2转录物水平在高CO2和低CO2条件二者中是相同的,并且显示在低CO2中没有明显的诱导。这与D66细胞形成对照,其中BST2转录物水平在低CO2条件中增加。
图2B示出在从高CO2条件转移至低CO2条件后在不同时间点D66中三种BST基因的表达。所有三种基因在转换到低CO2后2小时内具有增加的转录物水平并且保持这些表达的水平直到诱导后至少12小时。这些结果表明,在已知的诱导CCM的低CO2生长条件下,所有三种BST基因得到上调。进一步,在cia5突变体中没有观察到该上调,这提示bestrophin基因的表达通过CIA5得到调节。总结一起,这些结果指示bestrophin基因在CCM中的可能作用。
实施例4:叶绿体中bestrophin的定位
目前的CCM模型指示,叶绿体类囊体膜处的碳酸氢盐转运蛋白对碳酸氢盐摄取是必要的,但是这些转运蛋白还没有被鉴定。以下实施例描述了莱茵衣藻细胞内嵌合的bestrophin-Venus蛋白质的定位。选择荧光成像方案来鉴定叶绿体内的特异性定位,因为计算分析基于三种bestrophin蛋白质的前导序列预测了叶绿体靶向,而不是在叶绿体内特定性定位。
材料和方法
加荧光蛋白质标签:对于该实验,BST1、BST2或BST3的编码序列与Venus的编码序列融合以便生成融合蛋白质BST1-Venus、BST2-Venus和BST3-Venus。如Mackinder等,Cell171(1):133-147e114,2017中的,将BST1-3基因克隆且转化入衣藻菌株CC-4533。简言之,BST1-3基因的开放阅读框是从基因组DNA PCR扩增的(表2中列出的引物)且克隆入通过Gibson组装的具有C-端Venus-3xFLAG和PSAD启动子的pLM005。生成三种单独的构建体,一个用于每个BST。PSAD是高表达启动子,其驱动编码定位于莱茵衣藻中光合系统I的基质侧的丰富叶绿体蛋白质的细胞核基因(Fischer和Rochaix,2001)。对于转化,野生型培养物生长至中期对数期且浓缩至2x108个细胞mL-1。在电穿孔之前,将悬浮物与通过EcoRV线性化的构建的质粒混合。然后,将悬浮物铺板于TAP巴龙霉素(20μg mL-1)上,用于选择。生成三种单独的菌株:一种表达BST1-Venus、一种表达BST2-Venus、和一种表达BST3-Venus。使用Typhoon 8610扫描仪鉴定荧光克隆物。用于Venus的激光设置为用于激发的532nm和用于发射的555/20,并且叶绿体自身荧光在633nm处激发以670/30发射。
表2.用于生成加Venus-标签的荧光BST1-3蛋白质的引物
共焦显微术:然后使用共焦显微镜取得三种生成的菌株的荧光图像以便确定bestrophin蛋白质定位。鉴定的荧光克隆物在TAP培养基中异养地生长直到达到中期对数期。然后收获培养物并且在成像之前在Tris-基本培养基中再悬浮过夜。使用具有1.4NA的x63物镜用配备Airyscan模块的激光器-扫描显微镜LSM880(Zeiss)捕获图像。514nm和561nm处的氩激光分别用于Venus和叶绿体的激发。滤波器被设置在525–550nm处用于Venus发射且在620–670nm处用于叶绿体发射。叶绿体荧光用于定位在这些菌株中的叶绿体类囊体堆叠,并且Venus和叶绿体荧光的重叠用于鉴定bestrophin蛋白质相对于叶绿体类囊体堆叠的位置。多次重复成像。
结果
如图3A中显示,通过Venus信号指示的,所有三种bestrophin融合蛋白质在叶绿体内呈现非均质信号(在左列中显示)。当将Venus信号和叶绿体信号(在中间列中显示)融合时,叶绿体中的Venus信号和类囊体堆叠之间可看见重叠。所有三种BST-Venus融合蛋白质定位于叶绿体的类囊体膜。另外,蛋白核的类囊体小管示出在Venus信号和叶绿体信号之间相同重叠。这在图3B中清楚地描绘,其示出BST1-Venus菌株的放大蛋白核图像。箭头指示穿透蛋白核的、在类囊体小管中的蛋白核内部可看见BST1-Venus荧光的地方。这些结果都确认了计算机预测,bestrophin被靶向至叶绿体(基于bestrophin前导序列)且强烈地指示对于bestrophin蛋白质的类囊体定位。
实施例5:Bestrophin是在低CO2下莱茵衣藻生长所需的
以下实施例描述了减少三种bestrophin BST1、BST2和BST3的表达对莱茵衣藻的生长的效果。在CO2浓度和pH变化的生长条件下,三联bestrophin RNAi敲低菌株bsti-1的生长表型与WT菌株D66以及突变体菌株cia3和pmp1进行比较。两种突变体菌株在已知的CCM组分中具有突变;cia3菌株在CAH3中具有突变,CAH3为类囊体碳酸酐酶,而pmp1菌株在LCIB/LCIC中具有突变,LCIB/LCIC为基质θ碳酸酐酶。
材料和方法
使用RNAi的BST1、BST2和BST3的敲低:选择RNAi敲低方案以便同时靶向所有三种bestrophin基因。目前,仅bst3敲除菌株可从衣藻培养物保藏(Chlamydomonas CultureCollection)获得;bst1和bst2敲除菌株不可得。而且,如在实施例7中更详细地描述,bst3敲除菌株在低CO2条件下进行了测试且发现正常生长。这意味着仅已经测试了bst3敲除菌株并且生成三联敲除菌株的材料目前不可得。所以,RNAi敲低方案考虑是最佳方案。
用于敲低BST蛋白质的人工微小RNA构建体使用Molnar等,Plant J 58(1):165-174,2009的方案制造。简言之,Web MicroRNA Designer MD3)网站(http://wmd3.weigelworld.org/cgi-bin/webapp.cgi)用于设计与三个BST编码序列的“共同区域”中不同位置互补的两组寡聚物。设计了两个独立的构建体并且克隆入从衣藻资源中心获得的pChlamyRNA3int质粒。在表3中示出用于RNAi敲低的设计为靶向三种bestrophin的寡核苷酸(oligo)。从400个转化子分离两个三联敲低系(BST-RNAi系1和2或bsti-1和bsti-2)。寡聚物B1正向和反向用于生成bsti-1并且寡聚物B2正向和反向用于生成bsti-2。
表3.设计为靶向三种bestrophin的寡核苷酸。
通过电穿孔,将赋予巴龙霉素抗性(paraR)的携带AphVIII基因的pChlamyRNA3int质粒转化入D66(Shimogawara等,Genetics 148(4):1821-1828,1998)。在含有抗生素巴龙霉素(4μg mL-1;Invitrogen)的TAP琼脂培养基上选择转化子。然后,通过将它们复制铺板在MIN板上筛选抗性菌株的“低CO2下病态”表型。然后,将这些放置入高CO2腔室(空气中5%[v/v]CO2)和低CO2腔室(空气中0.01%[v/v]CO2)中,连续的照明(100μmol m-2s-1)7天。通过将生长细胞悬浮在液体MIN培养基中至相同的细胞密度(OD730=0.1、0.05和0.025)进行斑点测试并且将15μL点样在MIN板上。将这些板放置在高、环境和低CO2腔室7天。使用环境气体监视器(EGM-4,PP系统,Massachusetts)测量CO2浓度。
定量RT-PCR(qRT-PCR;qPCR):根据制造商的说明书使用Trizol试剂(Invitrogen)进行RNA提取。每个样品的1ug RNA用作cDNA的模板,cDNA根据制造商的说明书使用第一链cDNA合成试剂盒(NEB)进行制备。将每个样品的100ng RNA用于进行qRTPCR,使用QuantStudio 6根据制造商的说明书使用来自NEB的通用一步骤RT-qPCR试剂盒。用于qPCR的引物在表4列出;CBLP引物用作对照。
表4.用于qPCR分析的引物
基因名称
引物名称
引物序列
BST1
qBST1F
GCTGTGTGGCATTGAGGAGA(SEQ ID NO:130)
qBST1R
GGATGAGGCTGATGAGTCCG(SEQ ID NO:131)
BST2
qBST2F
ACGGTCTACGACTTCCCTCA(SEQ ID NO:132)
qBST2R
TTGGATCACGTGGGATTGGG(SEQ ID NO:133)
BST3
qBST3F
AAGTCAGCAAGGTTCCCTCG(SEQ ID NO:134)
qBST3R
TGAATGAGCCTAGCGGGTTG(SEQ ID NO:135)
CBLP
qCBLPF
ATGTGCTGTCCGTGGCTTTC(SEQ ID NO:136)
qCBLPR
CAGACCTTGACCATCTTGTCCC(SEQ ID NO:137)
在不同pH和CO2水平下的生长表型:WT菌株D66、突变体菌株cia3(CAH3敲除)、突变体菌株pmp1(LCIB/LCIC敲除)和bsti-1在非常低CO2(空气中0.01%CO2(v/v))、低(或环境)CO2(空气中0.04%-0.045%CO2(v/v))和高CO2(空气中5%CO2(v/v))下生长。在pH 7和pH8.4二者下测试这些三种CO2条件的每一种中的生长。为了接种板,使用三种不同的细胞浓度;最高浓度是10,000个细胞,中浓度是5,000个细胞和最低浓度是2,500个细胞。将三种不同浓度处的三种斑点应用于所有四种菌株的每种测试板。生长表型实验重复三次。
结果
为了检查在低CO2条件下莱茵衣藻生长中bestrophin的作用,采用RNAi方案以同时减少所有三种bestrophin基因的表达。靶向BST1、BST2和BST3的RNAi用于生成两种RNAi敲低系,bsti-1和bsti-2。使用定量RT-PCR测量bsti-1、bsti-2中和WT对照菌株D66中的BST1、BST2和BST3的表达水平。如图4中显示,与WT对照菌株D66相比,所有三种bestrophin基因的表达水平在bsti-1和bsti-2RNAi敲低系中更低(显示的误差条指示三次生物学重复的标准误差)。具体地,bsti-1和bsti-2(BST-RNAi系1和2)显示与D66相比,BST1、BST2和BST3的表达中几乎60-90%敲低。该结果指示使用RNAi方案有效地减少了bsti-1和bsti-2RNAi敲低系中所有三种bestrophin基因的表达。
接下来,测试了bsti-1RNAi敲低系、野生型菌株D66以及CAH3突变体菌株cia3和LCIB/LCIC突变体菌株pmp1在不同pH和CO2水平下生长的能力。培养物在7或8.4的pH和在非常低CO2、低CO2或高CO2条件下生长。在非常低CO2中bsti-1示出严重降低的生长,在高pH进一步加剧,类似于CCM突变体、cia3和pmp1的生长(图5A)。在pH 7,bsti-1的生长比在pH 8.4稍好。在低CO2中,与野生型D66菌株相比,bsti-1、cia3和pmp1都示出降低的生长(图5B)。此外,与pH 8.4相比,在pH 7下所有四种菌株示出稍好的生长。然而,在高CO2,bsti-1的生长与野生型、cia3和pmp1相当(图5C)。总结起来,这些结果示出,与WT(D66)和其他具有已知的低CO2病态表型(cia3,pmp1)突变体二者相比,所有三种bestrophin(bsti-1)的RNAi敲低显示出清楚的低CO2病态表型。而且,CAH3(cia3是CAH3敲除)是莱茵衣藻类囊体腔内发现的碳酸酐酶,并且是CCM发挥功能所需要的。三种bestrophin蛋白质可为将碳酸氢盐递送至类囊体内部的CAH3的蛋白质。因此,这些结果示出,所有三种BST是在低CO2条件下莱茵衣藻的野生型样生长所需的。
实施例6:三联bestrophin RNAi敲低菌株具有降低的积聚无机碳(Ci)的能力
以下实施例描述了与WT菌株D66相比,三联bestrophin RNAi敲低菌株bsti-1和bsti-2(实施例5中描述的)的无机碳亲和力测试。
材料和方法
无机碳的亲和力:对于该测定,在各种无机碳水平(无机碳=CO2&HCO3 -)下测量光合作用(如O2释放)的速率。根据Ma等,Plant Physiol156:884-896,2011评估了外部Ci(K1/2[DIC])(溶解的无机碳)的亲和力。具体地,将具有100μg叶绿体的等同物的细胞悬浮在用惰性氮气体鼓泡(即无CO2)的HEPES-NaOH缓冲液(pH 7.4)、HEPES-NaOH缓冲液(pH 7.8)或25mM EPPS-NaOH缓冲液(pH 8.4)中。将细胞转移至以300μmol m-2s-1照明的O2电极腔室(Rank Brothers,Cambridge UK),并且放置以耗尽在缓冲液和细胞内空间中的任何剩余DIC。在耗尽内源CO2时,没有观察到净O2释放。将已知浓度的NaHCO3注入腔室并且测量O2释放的速率。在该实验中,无机碳水平从25μM改变至2mM。计算K1/2[DIC]作为氧释放(即,光合作用)的半(50%)最大速率所需的DIC浓度(Badger,1985)。通过组合叶绿体a和b测量叶绿体含量。在100%甲醇中提取叶绿体并且使用分光光度计进行测量。取K1/2(CO2)作为达到半Vmax O2释放需要的CO2浓度。
评估在pH 8.4适应低CO2(<0.04%CO2)12小时的bsti-1和D66的Ci亲和力(图6A)。图6A中显示的K0.5(Ci)值(半最大氧释放所需的Ci浓度)是从图6B中显示的O2释放对Ci曲线进行计算的。另外,评估在pH 7.8适应低CO2(<0.04%CO2)12小时的bsti-1、bsti-2和D66的Ci亲和力(图6C)。图6C中显示的K0.5(Ci)值(半最大氧释放所需的Ci浓度)是从图6D中显示的O2释放对Ci曲线进行计算的。进一步,评估在pH 7.8适应高CO2(>5%CO2)12小时的bsti-1和D66的Ci亲和力(图6E)。图6E中显示的K0.5(Ci)值(半最大氧释放所需的Ci浓度)是从图6F中显示的O2释放对Ci曲线进行计算的。以每种Ci浓度进行三次重复运行。符号“*”指示K0.5(Ci)中的差异是显著的(通过student t检验的P<0.05)。在pH 7.8和低CO2适应情况下,D66的Vmax是121μmol O2 mg-1Chl hr-1、bsti-1的Vmax是105μmol O2 mg-1Chl hr-1和bsti-2的Vmax是95μmol O2 mg-1Chl hr-1。在pH 7.8和高CO2适应情况下,D66的Vmax是121μmol O2 mg-1Chl hr-1和bsti-1的Vmax是120μmol O2 mg-1Chl hr-1。在pH 8.4和低CO2适应情况下,D66的Vmax是124μmol O2 mg-1Chl hr-1和bsti-1的Vmax是85.5μmol O2 mg-1 Chl hr-1。所有菌株的Vmax被设置至100%氧释放活性。
无机碳的摄取:依据Moroney等,Plant Physiol 79(1):177-183,1985,将硅油离心用于测量溶解的Ci的细胞内浓度。简言之,将细胞离心并且以25μg Chl mL-1密度悬浮在Ci耗尽的25mM EPPS-NaOH(pH 7.8或8.4)中,并且在光中孵育直到净O2释放是零。细胞维持在光中直到被使用。然后,将300μL的Ci耗尽的细胞在含有用75μL的Dow Corning AR 20硅油覆盖的具有0.75%(w/v)SDS的25μL的1M甘氨酸(pH 10)的管中离心。在BeckmanMicrofuge B中,在200μmol m-2s-1光中在25℃进行测定。通过添加3μl的在pH 7.8,25mM(对于25μM的添加浓度)或在pH 8.4,50mM(对于50μM的添加浓度)的NaH14CO3启动Ci摄取,随后进行指示时间的光照(以150μmol m-2s-1的光15和120秒之间)。每个时间点运行一式三份样品。通过在microfuge B(Beckman)中离心15秒终止反应。使用沉淀中总的和酸稳定的14C之间的差异计算内部Ci并且如描述的校正细胞体积(Machingura等,J Exp Bot 68(14):3879-3890,2017)。
结果
具有CCM的藻类细胞的两个特点是,第一,它们对无机碳(Ci)的非常高的亲和力,和第二,它们积聚Ci至比通过扩散可获得的更高水平的能力。所以,测试RNAi敲低系bsti-1和bsti-2以及WT菌株D66的光合氧释放活性。如通过它们测量的K0.5(Ci)判断,在pH 7.8,适应低CO2的RNAi敲低系bsti-1和bsti-2分别展示出对Ci的亲和力低三至十倍(图6A和图6C)。当适应高CO2,与D66相比,bsti-1也具有Ci亲和力的降低(图6E)。这指示了降低所有三种BST基因的表达导致细胞对Ci亲和力的降低。在pH 8.4,bsti-1的K0.5(Ci)是95μM,与D66的35μM的低K0.5(Ci)形成鲜明的对照。在较高的pH 8.4,培养基中主要的Ci种类将为碳酸氢盐,因此D66(WT)细胞对Ci的较高亲和力反映了它们积极摄取和利用碳酸氢盐的能力。这些结果,示出bsti-1和bsti-2具有对用于光合O2释放的无机碳的更高需求,是bestrophin在CCM中的作用的有力的证据。
还测量了D66和bsti-1中Ci摄取活性,以评估BST1-3在积聚和固定Ci中的重要性。在pH 7.8以及pH 8.4二者,与D66相比,适应低CO2的bsti-1具有显著更低的14Ci积聚和固定(图7A-7D)。在pH 7.8和8.4二者,bsti-1积聚14Ci仅至在D66细胞中观察到的水平的20至25%。该差异在最早时间点(15秒)和在D66细胞已经使用完大部分添加的14Ci的最晚时间点(光中至多两个分钟)都可见。这些结果指示BST1-3在莱茵衣藻的低CO2条件中的Ci摄取中起重要作用,并且再次提供了CCM中bestrophin的作用的有力的证据。
实施例7:当与WT相比时,bst3敲除菌株具有在低CO2条件下的正常生长和类似的无机碳亲和力
以下实施例描述了与WT菌株D66相比,莱茵衣藻bst3敲除菌株在低CO2条件下的生长和Ci亲和力测试。
材料和方法
莱茵衣藻敲除菌株:敲除菌株使用随机插入诱变创建,并且是衣藻文库项目(CLiP)的部分。菌株可从衣藻资源中心(https://www.chlamycollection.org/)订货。目前,仅bst3敲除菌株是可获得的;bst1和bst2敲除菌株是不可获得的。为了确认bst3敲除菌株中的插入物位置,使用对BST3基因和对插入物特异性的引物进行PCR(参见表5)。插入物特异性引物(CIB1F和CIB1R)和侧面区域的信息从CLiP网站(https://www.chlamylibrary.org/allMutants)获得。
表5.用于分析bst3插入的引物
引物名称
引物序列
BST3F
TGCCCCTTCTCAGCACGT(SEQ ID NO:138)
BST3R
ACTGCCTCACACTCCCCT(SEQ ID NO:139)
CIB1F
GCACCAATCATGTCAAGCCT(SEQ ID NO:140)
CIB1R
GACGTTACAGCACACCCTTG(SEQ ID NO:141)
如实施例3中进行半定量RT-PCR分析。
生长测量:使用以上实施例中描述的方法,WT菌株D66和敲除菌株bst3在pH 8.6中生长。使用OD730测量生长,并且在波长645和663处进行叶绿体评估。测量在低CO2(<0.04%CO2)进行六天。在将细胞以0.01的OD730转移至MIN前,细胞在TAP中生长48小时。
无机碳的亲和力:使用实施例6中描述的方法进行。在pH 7.4测量氧释放活性并且K0.5(Ci)值(半最大氧释放所需的Ci浓度)是从O2释放对Ci曲线进行计算的。以每种Ci浓度进行一式三份运行。
结果
BST3敲除(bst3)从在bst3基因的第一外显子中具有巴龙霉素插入物的CLiP突变体保藏获得(图8A)。使用PCR确认插入物的位置(图8B)。另外,BST1-3表达在高和低CO2条件下的bst3敲除菌株中进行分析,并且与WT菌株D66相比。BST3表达在两种条件下的bst3中没有检测到,而BST1和BST2表达在两种条件下的bst3敲除菌株和WT菌株D66之间是相当的(图8C)。
与在空气中低(<0.04%CO2)(图9A-9B)、非常低(0.02%CO2)或高CO2(5%CO2(v/v))下的野生型细胞相比,观察到bst3菌株没有显著的生长差异(数据未显示)。WT和bst3之间的Ci亲和力中也没有明显的差异(图9C)。在RNAi敲低系bsti-1中,其中所有三种BST基因的表达是降低的,在pH 7.8和pH 8.4二者Ci亲和力降低。这与bst3形成鲜明的对比,敲除菌株仅缺失BST3表达,其与WT在Ci亲和力方面没有差异(图9C-9D)。这些结果支持三种BST的功能是冗余的和所有三种基因的表达必须减少以观察生理学效果的假说。
实施例8:BST1、BST2和BST3的结构特征和当前的莱茵衣藻CCM模型
以下实施例描述了莱茵衣藻BST1、BST2和BST3蛋白质的结构特征。实施例还描述了当前的莱茵衣藻CCM模型。
材料和方法
从Phytozome v12.1获得BST1、BST2和BST3的肽序列。使用肺炎克雷伯杆菌bestrophin(Kpbest;PDB:4DW8)作为模板进行同源性建模,并且用Swiss-Model网络服务器产生(Yang等,Science 346(6216):1498-1501,2014)。选择Kpbest,因为它通过Swiss-Model鉴定为的BST1-3的最高排名模板。获得的结构和Kpbest的结构展示为单体,具有在选择性孔内层的保守的残基在选择性孔内层的保守的残基,如在图10A中显示为伸出形状并标记的。提交使获得的BST1均五聚物模型至用gromos 43B1力场的能量最小化并且使用Swiss-PDBviewer(V4.01)使用原子部分电荷计算静电势。然后,电场在BST1均五聚物模型上展示以产生图10D。静电势在-4kT/e(负)至+4kT/e(正)的电势范围中展示,并且突显腔。使用QMEAN评分(Benkert等,蛋白质71(1):261-277,2008)获得BST1均五聚物结构质量(图10B)。
结果
为了预测是否BST1-3可用作阴性离子转运bestrophin,使用肺炎克雷伯杆菌的bestrophin进行BST1-3的同源性建模。BST1-3含有沿着他们在bestrophin家族的蛋白质中保守的选择性孔的非极性残基(图10A;Qu等,J Neurosci 26(20):5411-5419,2006)。结构研究显示人和肺炎克雷伯杆菌bestrophin是五聚物,并且五聚物组装中BST1的建模是高可信度的(图10B)。进入袋主要具有负静电势并且选择性孔是中性/带正电的,支持BST1-3转运带负电的离子的假说(图10C-10D;Yang等,Science 346(6216):1498-1501,2014;Kane等,Nature 516(7530):213-218,2014)。这些结果提示莱茵衣藻bestrophin可为离子转运蛋白。
实施例2-8中描述的结果证明对莱茵衣藻CCM来说三种bestrophin蛋白质的重要作用。总结起来,这些结果指示三种bestrophin蛋白质是类囊体定位的碳酸氢盐转运通道。图11示出BST1、BST2和BST3整合入用于Ci转运的当前的莱茵衣藻CCM模型。
序列表
<110> 路易斯安那州立大学监事会和农机学院,英国约克大学
<120> 绿藻的bestrophin碳酸氢盐转运蛋白
<130> 79454-20001.40
<140> 尚未分配
<141> 与此同时
<150> US 62/769,214
<151> 2018-11-19
<150> US 62/697,840
<151> 2018-07-13
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1 5 10 15
Arg Leu Pro Val Arg Thr Ser Arg Val Cys Gln Leu Ser Val Arg Cys
20 25 30
Gln Ala Ala Asn Lys Asp Pro Asn Ala Pro Ile Gln Ser Asn Pro Leu
35 40 45
Gly Ser Phe Ser Ser Gln Asn Ser Ser Gly Ala Val Val Thr Ala Pro
50 55 60
Arg Asn Glu Asp Ala Arg Lys Tyr Phe Arg Thr Val Tyr Asp Phe Pro
65 70 75 80
Gln Trp Gln Lys His Arg Ser Gln Ser Arg Leu Val Arg Arg Leu Phe
85 90 95
Thr Ile Pro Gln Ser His Val Ile Gln Asn Ala Leu Pro Ser Ile Met
100 105 110
Trp Val Thr Phe Thr Ser Thr Cys Val Ala Ala Tyr Met Tyr Gly Tyr
115 120 125
Asp Leu His Ile Leu Pro Glu Gly Phe Pro Thr Leu Ala Pro Asn Ala
130 135 140
Ala Cys Ser Ala Phe Ile Ser Asn Thr Ser Val Ala Leu Ser Leu Leu
145 150 155 160
Leu Val Phe Arg Thr Asn Ser Ser Tyr Gly Arg Trp Asp Glu Ala Arg
165 170 175
Lys Met Trp Gly Gly Leu Leu Asn Arg Ser Arg Asp Ile Met Arg Gln
180 185 190
Gly Ala Thr Cys Phe Pro Asp Asp Gln Val Glu Ala Lys Lys Ala Leu
195 200 205
Ala Arg Trp Thr Val Ala Phe Ala Arg Ala Leu Arg Ile His Phe Gln
210 215 220
Pro Glu Val Thr Ile Glu Ser Glu Leu Gln Asn Ile Leu Thr Pro Ala
225 230 235 240
Glu Leu Gln Met Leu Ala Lys Ser Gln His Arg Pro Val Arg Ala Ile
245 250 255
His Ala Ile Ser Gln Ile Ile Gln Ser Val Arg Met Ser Ser Ile His
260 265 270
Gln Gln Gln Met Ser Asn Asn Leu Thr Phe Phe His Asp Val Leu Gly
275 280 285
Gly Cys Glu Arg Leu Leu Arg Ala Pro Ile Pro Val Ser Tyr Thr Arg
290 295 300
His Thr Ala Arg Phe Leu Phe Ala Trp Leu Thr Leu Leu Pro Phe Ala
305 310 315 320
Leu Tyr Gly Ser Cys Gly Val Ser Val Ile Pro Val Cys Thr Gly Ile
325 330 335
Ala Ala Val Leu Cys Gly Ile Glu Glu Ile Gly Val Gln Cys Glu Glu
340 345 350
Pro Phe Gly Ile Leu Pro Leu Asp Val Ile Cys Asn Arg Ile Gln Ala
355 360 365
Asp Val Met Ala Thr Leu Lys Asp Asp Ala Asp Thr Lys Thr Val Leu
370 375 380
Ala Glu Ala Gly Leu Ile Ser Leu Ile Pro Ser Met Ser Leu Pro Pro
385 390 395 400
Thr Glu His Ala Ser Pro Ser Asp Pro Val Thr Ala Ala Ala Ala Ala
405 410 415
Ala Leu Ala Ala Ala Asn Gly Asn Gly Ala Ala Ser His Ser Asn Gly
420 425 430
Asn Gly Ser Lys Pro Val Ser Thr Gln Val Pro Pro Pro Val Leu Ala
435 440 445
Pro Val Thr Val Thr Ser Ser Ser Gly Ser Met Asn Val Arg Ile Ser
450 455 460
Pro Arg
465
<210> 4
<211> 325
<212> PRT
<213> 螺旋孢子虫属 ATCC 50920
<400> 4
Met Leu Ala Ala Thr Asn Arg Ser Ala Ser Ser Pro Pro Lys Pro Pro
1 5 10 15
Phe Glu Gln Lys Gly Tyr Phe Pro Gly Ser Trp Pro His Ala Thr Phe
20 25 30
Asp Leu Ser Gln Gly Phe Tyr Ser Cys Thr Phe Ala Leu Ser Leu Leu
35 40 45
Leu Val Phe Arg Thr Asn Ala Ala Tyr Asp Arg Trp Trp Glu Ala Arg
50 55 60
Lys Thr Trp Gly Ser Leu Val Asn Arg Thr Arg Asp Phe Met Arg Gln
65 70 75 80
Ala Leu Thr Phe Phe Pro Ala Ser Glu Ser Ala Glu Ala Gly Val Leu
85 90 95
Leu Arg Trp Thr Val Ala Leu Pro Arg Met Leu Leu Trp Lys Leu Arg
100 105 110
Glu Gln Ala Val Leu Glu Ala Glu Leu Gln Gly Val Leu Leu Pro Ala
115 120 125
Glu Phe Ser Leu Leu Arg Glu Cys Asp His Arg Cys Ala Leu Ala Leu
130 135 140
Ala Leu Met Ser Glu Val Val Gln Arg Ala Arg Gly Leu Asp Arg Gly
145 150 155 160
Gln Arg Arg Cys Leu Asp Ala Asn Leu Thr Ala Leu Gly Asp Thr Val
165 170 175
Gly Val Cys Glu Arg Ile Leu Arg Thr Pro Ile Pro Leu Ser Tyr Ser
180 185 190
Arg His Thr Ser Arg Trp Leu Leu Val Trp Leu Ala Phe Leu Pro Phe
195 200 205
Ser Leu Trp Arg Leu Tyr Ala Trp Gly Ser Val Pro Leu Ser Gly Leu
210 215 220
Leu Ala Phe Leu Met Leu Gly Ile Asp Glu Ile Gly Val Gln Ile Glu
225 230 235 240
Gln Pro Phe Gln Val Leu Pro Leu Glu Ser Leu Cys Cys Thr Val Glu
245 250 255
Arg Asn Leu Arg Asn Leu Ala Ala Ala Thr Pro Ala Val Lys Arg Leu
260 265 270
Ala Ser Leu Ala Val Glu Ala Gln Leu Ala Leu Ala Glu Ser Asp Ala
275 280 285
Thr Ala Ser Phe Arg Glu Pro Gly Lys Ser Leu Ala Cys Ser Val Phe
290 295 300
Pro Pro Val Ser Pro Arg Ser Ser Leu Ile Arg Ser Cys Leu Asp Arg
305 310 315 320
Pro Gln Ser Leu Pro
325
<210> 5
<211> 422
<212> PRT
<213> 圆果种黄麻
<400> 5
Met Ala Gln Ser Ser Asn Pro Ser Lys Leu Ser Leu Ser Ser Asp Phe
1 5 10 15
Asn Pro Lys Leu Phe Pro Asn Lys Leu His Arg Tyr Ser Ser Phe Pro
20 25 30
Phe Lys Leu Ser Gln Pro Arg Ser Phe Lys Thr Leu Ser Ser Leu Ser
35 40 45
Pro Pro Pro Pro Pro Ser Ser Pro Pro Pro Pro Ser Ser Glu Pro Thr
50 55 60
Thr Thr Lys Thr Leu Asn Leu Val Ser Leu Leu Arg Ala Ile Pro Asp
65 70 75 80
Trp Ala Asp Arg Val Gln Glu Arg Gly Met Arg Gln Asn Arg Ala Leu
85 90 95
Tyr Asn His Asp Lys Trp Ser Glu His Arg Ser Ser Leu Arg His Leu
100 105 110
Arg His Leu Leu Ser Ser Leu Gln Ser Arg Val Ile Leu Ser Leu Val
115 120 125
Pro Pro Val Leu Ala Phe Thr Ser Val Ala Ala Val Ile Ala Thr Tyr
130 135 140
Asn Thr Ala Val Asp Leu His Trp Leu Pro Gly Phe Phe Pro Val Leu
145 150 155 160
Arg Ala Ser Ser Leu Pro Tyr Gln Leu Thr Ala Pro Ala Leu Ala Leu
165 170 175
Leu Leu Val Phe Arg Thr Glu Ala Ser Tyr Ser Arg Phe Glu Glu Gly
180 185 190
Arg Lys Ala Trp Thr Lys Val Ile Ser Gly Thr Asn Asp Phe Ala Arg
195 200 205
Gln Val Ile Ser Gly Val Glu Asn Ser Ser Asp Asp Gln Ser Leu Lys
210 215 220
Asp Ala Leu Leu Arg Tyr Ile Met Ala Phe Pro Val Ala Leu Lys Cys
225 230 235 240
His Val Met Tyr Gly Ser Asp Ile Gly Arg Asp Leu Gln Asn Leu Leu
245 250 255
Glu Val Asp Asp Leu Thr Val Val Leu Asn Ser Lys His Arg Pro Arg
260 265 270
Cys Ile Ile Asp Phe Ile Ser His Ser Leu Arg Val Leu Asn Leu Lys
275 280 285
Glu Ser Glu Arg Thr Val Leu Glu Ser Lys Ile Ser Cys Phe His Glu
290 295 300
Gly Ile Gly Val Cys Glu Gln Leu Met Gly Ile Pro Ile Pro Leu Ser
305 310 315 320
Tyr Thr Arg Leu Thr Ser Arg Phe Leu Val Leu Trp His Leu Thr Leu
325 330 335
Pro Ile Ile Leu Trp Asp Asp Cys His Trp Ile Val Val Pro Ala Thr
340 345 350
Phe Ile Ser Ala Ala Ser Leu Phe Cys Ile Glu Glu Val Gly Val Leu
355 360 365
Ile Glu Glu Pro Phe Pro Met Leu Ala Leu Asp Glu Leu Cys Asn Val
370 375 380
Val Gln Asn Asn Ile Arg Glu Ala Ile Ala Thr Glu Lys Val Ile Lys
385 390 395 400
Ala Lys Leu Ile Gly Lys Arg Lys Arg His Ser Tyr Lys His Ser Pro
405 410 415
Asn Gly Trp Pro Asn Thr
420
<210> 6
<211> 406
<212> PRT
<213> 地钱土生亚种
<400> 6
Met Glu Asp Val Arg Leu Gly Arg Cys Glu Gly Ala Glu Gly Val Gly
1 5 10 15
Lys Met Gly Lys Trp Val His Lys Leu Ala Arg Phe Tyr Ala Pro Cys
20 25 30
Ala Leu Pro Leu Leu Cys Val Thr Met Gly Leu Trp Ile Pro Glu Ser
35 40 45
Pro Gly Ser Arg Ser Gly Arg Ser Gly Pro Glu Pro Glu Pro Tyr Asp
50 55 60
Pro Asp Phe Pro Lys Glu Glu Val Phe Leu Tyr Arg Arg Thr Val Tyr
65 70 75 80
Asp His Lys Asp Trp Ser Arg His Arg Ser Ser Leu Arg His Ser Arg
85 90 95
His Ile Leu Ser Met Arg Ser Ser Arg Val Ile Leu Ala Leu Trp Pro
100 105 110
Pro Val Phe Gly Leu Thr Thr Val Ser Ile Ala Leu Ser Ala Tyr Asn
115 120 125
Glu Cys Ile Leu Ser His Trp Leu Pro Ser Phe Leu Pro Leu Leu His
130 135 140
Val Ser Ala Thr Pro Phe Gln Leu Met Ala Pro Ala Leu Ala Leu Leu
145 150 155 160
Leu Val Phe Arg Thr Asn Ala Ser Tyr Ala Arg Phe Asp Glu Ala Arg
165 170 175
Arg Ala Trp Gly Ser Asn Val Asn Arg Thr Arg Asp Ile Thr Arg Gln
180 185 190
Ala Leu Thr Trp Met Gln His Pro Ser Asp Ala Glu Lys Val Lys Lys
195 200 205
Leu Ile Arg His Cys Val Ala Phe Asn Val Cys Met Lys His His Leu
210 215 220
Val Arg Gly Gly Asp Leu Arg Asp Asp Leu His Ser Trp Ile Asp Lys
225 230 235 240
Glu Glu Ile Asp Gly Ile Leu Ala Ser Thr His Arg Pro Asn Tyr Val
245 250 255
Leu Gln Val Met Ser Glu Ile Ile His Ser Cys Ser Ile Thr Glu Met
260 265 270
Gln Leu Thr Arg Met Asp Val Asn Met Thr Gln Phe Ala Asp Asn Leu
275 280 285
Gly Ala Cys Glu Arg Ile Phe Lys Thr Pro Ile Pro Leu Ser Tyr Thr
290 295 300
Arg Leu Thr Ser Arg Phe Leu Val Leu Trp His Ile Ala Leu Pro Leu
305 310 315 320
Ala Leu Trp Asp His Cys Gln Trp Val Ser Val Pro Ala Thr Phe Leu
325 330 335
Ser Ala Gly Ala Leu Phe Cys Ile Glu Glu Val Gly Val Leu Ile Glu
340 345 350
Glu Pro Phe Gln Ile Phe Pro Leu Asp Asn Ile Cys Ser Thr Ile Lys
355 360 365
Lys Asn Val Asp Gly Leu Ile Leu Ala His Thr Glu Ile His His Cys
370 375 380
His Lys His Pro Pro Lys Gly Cys Asp Ile Pro Lys Lys Lys Ser Ser
385 390 395 400
Gly Asp Ala Pro Lys Ser
405
<210> 7
<211> 453
<212> PRT
<213> 微芒藻
<400> 7
Met Thr Cys Leu Thr Ser Val Leu Pro Leu Gly Gly Ala Leu Val Pro
1 5 10 15
Thr Arg Pro Leu Arg Ser Gly Ile His Ser Ser Thr Pro Arg Arg Arg
20 25 30
Leu Ser Ala Leu Ala Pro Leu Lys Pro His Leu Val Ala Glu Pro Glu
35 40 45
Ser Val Gln His Ala Ala Pro Ala Ala Ala Ala Pro Pro Pro Pro Pro
50 55 60
Pro Arg Asp Ala Thr Pro Ser Ala Pro Arg Gln Pro Leu Phe Gly Asp
65 70 75 80
Ala Leu Leu Glu Ser Thr Arg Pro Gln Leu Arg Thr Val Phe Asp Ala
85 90 95
Asp Arg Trp Val Met His Arg Ser Val Asn Arg Tyr Phe Arg His Leu
100 105 110
Trp Asp Leu Pro Arg Ser Arg Leu Leu Leu Gly Leu Ala Ala Pro Leu
115 120 125
Ala Tyr Val Leu Gly Leu Ser Ala Val Val Cys Ala Tyr Gly Ala Ala
130 135 140
Gln Gln Ala Gly Leu Leu Pro Ser Leu Leu Pro Ser Leu Ala Gly Ala
145 150 155 160
Ala Arg Met Arg Glu Leu Tyr Asn Leu Thr Ser Pro Thr Leu Ala Leu
165 170 175
Leu Leu Val Phe Arg Thr Asn Ala Ser Tyr Ala Arg Trp Asp Glu Gly
180 185 190
Arg Lys Met Trp Gly Met Val Leu Asn Arg Thr Arg Asn Ile Cys Arg
195 200 205
Leu Gly Leu Ala Trp Ile Gly Asp Asp Lys Arg Glu Leu Arg Ser Met
210 215 220
Leu Glu Glu Leu Glu Gly Ile Leu Leu Pro His Glu Ile Glu Gly Val
225 230 235 240
Leu Arg Ala Ser His Arg Pro Asn Tyr Val Leu Gln Val Leu Ala Gln
245 250 255
Ile Val Arg Thr Ala Gly Leu Pro Thr Ala Ala Thr Leu Arg Met Glu
260 265 270
Asp Asp Leu Thr Asn Phe Gly Asp Ser Leu Gly Gly Cys Glu Arg Leu
275 280 285
Leu Arg Thr Pro Ile Pro Leu Phe Tyr Thr Arg His Thr Ser Arg Phe
290 295 300
Leu Met Ile Trp Leu Thr Phe Leu Pro Ala Thr Leu Trp Pro Ala Cys
305 310 315 320
Gly Leu Leu Thr Leu Pro Leu Val Phe Leu Ile Ser Phe Leu Leu Leu
325 330 335
Gly Val Asp Lys Ile Gly Val Ser Ile Glu Glu Pro Phe Ser Ile Leu
340 345 350
Glu Leu Glu Thr Ile Ala Ser Arg Ala Leu Glu Asn Val His Glu Leu
355 360 365
Ala Ala Met His Asp Gly Val Ala Asp Ile Pro Leu Gly Gly Gly Ser
370 375 380
Ser Ser Ser Ser Ser Ser Asn Arg Ala Ser Ser Asn Arg Gly Ala Gly
385 390 395 400
Thr Gly Arg Leu His Pro Ser Phe Gly Val Tyr Ser Ser Ser Asn Gly
405 410 415
Ser Ser Gly Ala Asn Gly Ser Gly Gly His His Gly Ser Gly Leu Ile
420 425 430
Gly His Val Ser Ser Ile Val Ser Ala Ala Thr Met Val Ser Leu Ala
435 440 445
Arg Pro Ala Thr Ala
450
<210> 8
<211> 803
<212> PRT
<213> 小球藻
<400> 8
Met Pro Ala Ala Leu Val Ala Ala Ala Ala Ala Thr Ala Cys Pro Ala
1 5 10 15
Ala Met Gly Arg Ala Pro Ala Ala Ala Gly Ser Ala Ser Arg Leu Pro
20 25 30
Ala Gly Leu Val Pro Ser Ser Gln Gln Leu Arg Trp Thr Leu Ala Pro
35 40 45
Gln Arg His Ser Gln Pro Ser Arg Cys Arg Arg Ala Ala Arg Val Ala
50 55 60
Ala Val Ala Gly Pro Gln Ala Pro Pro Arg Leu Gly Ala Pro Leu Gln
65 70 75 80
Leu Gly Ser Pro Ala Ser Gln Lys Arg Arg Glu Glu Ile Leu Asn Arg
85 90 95
Ile Asp Thr Leu Ile Gln Glu Glu Leu Glu Ser Gln Leu Pro Leu Pro
100 105 110
Ser Val Asp Glu Leu Val Ala Asp Asp Phe Lys Glu Ser Glu Arg Arg
115 120 125
Lys Leu Arg Thr Val Phe Asp Phe Asp Leu Trp Lys Arg His Arg Ser
130 135 140
Ser Ser Arg Tyr Ser Arg His Met Phe Gly Leu Leu Glu Ser Arg Thr
145 150 155 160
Val Arg Trp Ile Gly Ala Pro Leu Ala Tyr Cys Val Ser Leu Ser Val
165 170 175
Ala Val Gly Val Tyr His Asn Leu Ala Asp Ala Gly Ile Val Pro Glu
180 185 190
Val Leu Pro Glu Leu Lys Thr Gln Leu Pro Ile Gln Leu Thr Ser Ala
195 200 205
Ala Leu Ser Thr Leu Leu Val Leu Arg Thr Asn Thr Ser Tyr Gln Arg
210 215 220
Trp Asp Glu Ala Arg Lys Met Trp Gly Leu Ile Val Asn Arg Ser Arg
225 230 235 240
Asp Phe Thr Arg Gln Ala Leu Gly Tyr Ile Pro Asp Asp Gln Pro Glu
245 250 255
Leu Gln Asp Met Leu Cys Arg Trp Val Val Ala Tyr Ser Arg Ser Leu
260 265 270
Met Cys His Leu Arg Ser Asp Glu Asp Leu Tyr Arg Glu Leu Ala Thr
275 280 285
Lys Leu Lys Pro Gln Glu Leu Glu Ala Leu Met Ser Ser Ser His Arg
290 295 300
Pro Asn Tyr Thr Val Gln Val Leu Thr Ala Ile Met Gln Ala Ala Lys
305 310 315 320
Leu Pro Gly Val Asp Ile Asp Pro Arg Asp Ser Gly Ala Asn Val Lys
325 330 335
Ala Ser Ala Leu Val Arg Met Asp Glu Gln Leu Thr Gln Phe Ala Asp
340 345 350
Val Thr Gly Gly Cys Glu Arg Ile Leu Arg Thr Pro Val Pro Leu Ala
355 360 365
Tyr Ser Arg His Asn Ser Arg Met Leu Thr Leu Trp Met Thr Leu Leu
370 375 380
Pro Phe Ser Leu Trp Asp Thr Cys Gly Trp Ala Thr Pro Phe Val Val
385 390 395 400
Leu Ile Val Ala Phe Leu Leu Leu Gly Val Lys Glu Ile Gly Leu Asn
405 410 415
Ile Glu Glu Pro Phe Ser Ile Leu Pro Leu Glu Thr Ile Cys Asn Thr
420 425 430
Ile Glu Thr Asn Val Trp Glu Leu His Arg Thr His Ser Thr Ala Ala
435 440 445
Ala Glu Ala Gln Gln Glu Ala Ala Arg Arg Gly Ser Trp Ser Gln Gln
450 455 460
Ala Gln Gln Gly Pro Gln Gln Val Asp Ala Asp Leu Leu Ala Pro Ala
465 470 475 480
Ala Ala Thr Gln Leu Gly Val Ala Leu Trp Arg Phe Pro Thr Gly Ala
485 490 495
Ala Tyr Leu Leu Ser Arg Thr Asn Leu Ala Ser Leu Ile Glu Arg Asn
500 505 510
Leu Arg Leu Arg Arg Gln Leu Gly Ile Ser Asp Ala Ala Thr Ala Thr
515 520 525
Ala Leu Phe Gln Ser Gln Gly Ala Leu Val Ser Lys Phe Glu Arg Ala
530 535 540
Glu Val Met Val Ala His Leu Gln Arg Leu Gln Ala Ser Gly Glu Leu
545 550 555 560
Ser Ala Glu Gln Val Ala Arg Met Ala Leu Ala Pro Ser Ala Leu His
565 570 575
Leu Thr Pro Ala Glu Phe Asp Arg Arg Trp His Asp Gly Gly Leu Ser
580 585 590
Arg Pro Gly Ala Ser Pro Thr Asp Ile Thr Met Ser Asp Thr Ala Gly
595 600 605
Arg Gln Gln Ala Ala Ala Ala Ala Leu Gly Glu Leu Leu Gln Ala Ala
610 615 620
Asp Gly Val Pro Gly Pro Leu Gln Pro Gln Tyr Leu Gln Gln Gly Glu
625 630 635 640
Glu Leu Val Gln Arg Ser Ala Gly Leu Trp Arg Ala Gly Pro Ala Ala
645 650 655
Leu Arg Ala Gly Trp Ala Ser Leu Gln Gln Leu Gly Leu Ser Asp Ser
660 665 670
Gln Val Val Ala Ala Met Thr Met Gln Pro Ala Val Leu Ser Leu Asp
675 680 685
Trp Ala Gly Glu Thr Lys Gln Arg Leu Leu Ala Trp Ala Glu Glu His
690 695 700
Leu Gly Ile Asp Thr Phe Gly Phe Leu Thr Ser Tyr Thr Arg Phe Val
705 710 715 720
Thr Tyr Ser Gly Ala Ser Val Ala Met Arg Ala Asp Phe Leu Gln Gln
725 730 735
His His Pro Ala Met Trp Asp Gln Asn Leu Cys Arg Gly Pro Arg Thr
740 745 750
Leu Phe Arg Leu Leu Thr Glu Pro Glu Arg Leu Cys Ala His Ala Gly
755 760 765
Cys Thr Glu Thr Glu Leu Val Ala Phe Asn Arg Ala Trp Leu Ala Thr
770 775 780
Pro Ala Gly Leu Arg Trp Gly Gly Lys Ala Gly Arg Ser Gln Arg Arg
785 790 795 800
Ala Ala Ser
<210> 9
<211> 417
<212> PRT
<213> 紫花风铃木
<400> 9
Met Lys Ile Ser Pro Ser Ser Cys Ser Pro Gln Phe Leu Pro Arg Thr
1 5 10 15
Leu Phe Lys Phe His His Ser Pro Leu Pro Phe Ile Ser Lys Lys Pro
20 25 30
His Lys Leu Ser Phe Lys Phe Ile Cys Ala Cys Gln Ser Ser Asn Asp
35 40 45
Ser Asn Pro Ser Pro Ser Gln Asp Phe Thr Leu Leu Ser Leu Leu Leu
50 55 60
Ala Ile Pro Asn Trp Ala Asp Gly Ile Lys Glu Arg Arg Met Lys Gln
65 70 75 80
Lys Arg Ser Leu Tyr Asp His Lys Ser Trp Val Gln His Arg Ser Ser
85 90 95
Leu Arg His Val Arg His Phe Leu Ser Ser Leu Ser Ser Arg Val Ile
100 105 110
Leu Ser Leu Val Pro Pro Val Ile Val Phe Thr Leu Val Ala Val Ile
115 120 125
Ile Ala Ser Tyr Asn Ser Ala Val Ser Ile His Trp Leu Pro Glu Phe
130 135 140
Phe Pro Ile Leu Arg Ala Ser Ser Leu Pro Tyr Gln Leu Thr Ala Pro
145 150 155 160
Ala Leu Ala Leu Leu Leu Val Phe Arg Thr Glu Ala Ser Tyr Ser Arg
165 170 175
Phe Glu Glu Gly Lys Lys Ala Trp Thr Lys Val Ile Ser Gly Thr Asn
180 185 190
Asp Phe Ala Arg Gln Val Ile Ala Ser Val Glu Ser Pro Ser Asp Ser
195 200 205
Met Leu Lys Lys Ala Ile Leu Gln Tyr Ile Met Ala Phe Pro Val Ala
210 215 220
Leu Lys Cys His Ile Ile Tyr Gly Ser Asp Ile Ala Gln Asp Leu Lys
225 230 235 240
Asn Leu Leu Glu Ala Asp Asp Leu Ser Val Val Leu Ser Ser Lys His
245 250 255
Arg Pro Arg Cys Ile Ile Glu Phe Ile Ser Gln Ser Leu Gln Leu Leu
260 265 270
Asp Met Asp Ala Thr Lys Leu His Val Leu Glu Ser Lys Leu Cys Cys
275 280 285
Phe His Glu Gly Ile Gly Val Cys Glu Gln Leu Met Gly Thr Pro Ile
290 295 300
Pro Leu Ser Tyr Thr Arg Leu Thr Ser Arg Phe Leu Ile Leu Trp His
305 310 315 320
Leu Thr Leu Pro Val Ile Leu Trp Asp Asp Cys His Trp Ile Val Val
325 330 335
Pro Ala Thr Phe Ile Ser Ala Ala Ser Leu Phe Cys Ile Glu Glu Val
340 345 350
Gly Val Leu Ile Glu Glu Pro Phe Pro Met Leu Ala Leu Asp Glu Leu
355 360 365
Cys Leu Thr Ala Gln Ser Asn Ile Gln Asp Ile Met Arg Asn Glu Lys
370 375 380
Leu Ile Lys Asp Gln Val Lys Lys Ala Lys Arg Lys Ile His Ser Arg
385 390 395 400
Lys His Ser Ser Asn Gly Trp Pro Asn Pro Leu Glu Asp Arg Gln Pro
405 410 415
Ser
<210> 10
<211> 385
<212> PRT
<213> 耐酸绿藻
<400> 10
Met Leu Val His Arg Val His Thr Arg Ser Leu Gly Asn Arg Asn Gln
1 5 10 15
Cys Gly Arg Lys Leu His Arg Val Ser Thr Phe Val Val Lys Thr Pro
20 25 30
Thr Glu Lys Pro Val Val Ala Asp Tyr Val Leu Pro Arg Ser Glu Glu
35 40 45
Ala Arg Arg Tyr Phe Arg Thr Val Tyr Asp Phe Pro Gln Trp Gln Lys
50 55 60
His Arg Ser Pro Thr Arg Leu Ile Asp Arg Leu Leu Gln Ile Pro Arg
65 70 75 80
Ser His Val Leu Gln Asn Ile Leu Pro Ser Ile Ala Trp Cys Ser Ser
85 90 95
Val Ala Gly Leu Leu Thr Leu Tyr Met Gln Ala Tyr Asp Ala His Ile
100 105 110
Leu Pro Asp Gly Phe Pro Ser Phe Ala Thr Asn Asn Ala Cys Thr Ser
115 120 125
Phe Val Asn Thr Thr Thr Val Ala Leu Ser Leu Leu Leu Val Phe Arg
130 135 140
Thr Asn Val Ser Tyr Gly Arg Trp Asp Glu Ala Arg Lys Met Lys Gly
145 150 155 160
Leu Leu Val Asn Arg Ser Arg Asp Leu Met Arg Gln Val Cys Ala Met
165 170 175
Val Pro Glu Glu Asp Val Ala Thr Lys Ala Met Met Ala Lys Trp Thr
180 185 190
Ala Ala Phe Cys Arg Val Leu Arg Ile His Phe Gln Pro Glu Val Ser
195 200 205
Leu Glu Asp Glu Met Lys Gly Leu Leu Ser Pro Glu Glu Leu Glu Trp
210 215 220
Leu Ile Glu Ser Lys His Arg Pro Cys Ser Val Ile His Met Leu Ser
225 230 235 240
Gln Ile Ile Tyr Asp Ser Gln Ile Ser Ala Ile Cys Gln Ala Gln Met
245 250 255
Cys Asn Asn Leu Thr Ala Phe Glu Asp Val Leu Gly Gly Cys Glu Arg
260 265 270
Leu Leu Arg Ala Pro Ile Pro Val Ser Tyr Thr Arg His Thr Ala Arg
275 280 285
Phe Leu Phe Thr Trp Leu Thr Leu Leu Pro Phe Ala Leu Tyr Asn Ser
290 295 300
Cys Gly Val Trp Thr Leu Pro Val Val Ala Gly Val Ser Ala Val Leu
305 310 315 320
Cys Gly Ile Glu Glu Ile Gly Val Gln Ile Glu Glu Pro Phe Gly Ile
325 330 335
Leu Pro Leu Glu Ala Ile Cys Gly Arg Ile Gln Ala Asp Val Met Ala
340 345 350
Thr Leu Lys Glu Asp Ala Lys Thr Arg Asn Leu Arg Asn Lys Val Leu
355 360 365
Leu Ile Arg Gly Pro Glu His Trp Ala Met Val Asn Gly His Ser Asn
370 375 380
Lys
385
<210> 11
<211> 397
<212> PRT
<213> 耐酸绿藻
<400> 11
Met Asn Lys Ser Leu Leu Arg Ala Pro Glu Ser Asn Val Val Phe Arg
1 5 10 15
Arg Ala Cys Asn Lys Ser Phe Val Lys Arg Cys Lys Glu Val Gln Arg
20 25 30
Leu Pro Ser His Val Ala Leu Ala Val Lys Asn Pro Ala Glu Lys Pro
35 40 45
Val Val Ala Asp Tyr Val Leu Pro Arg Ser Glu Glu Ala Arg Arg Tyr
50 55 60
Phe Arg Thr Val Tyr Asp Phe Pro Gln Trp Gln Lys His Arg Ser Gln
65 70 75 80
Leu Arg Leu Ile Asp Arg Leu Leu Gln Ile Pro Arg Ser His Val Leu
85 90 95
Gln Asn Ile Leu Pro Ser Ile Ala Trp Cys Ser Ser Val Ala Gly Leu
100 105 110
Leu Thr Leu Tyr Met Gln Ala Tyr Asp Ala His Ile Leu Pro Asp Gly
115 120 125
Phe Pro Ser Phe Thr Pro Asn Asn Ala Cys Thr Ser Phe Val Asn Thr
130 135 140
Thr Thr Val Ala Leu Ser Leu Leu Leu Val Phe Arg Thr Asn Val Ser
145 150 155 160
Tyr Gly Arg Trp Asp Glu Ala Arg Lys Met Lys Gly Leu Leu Val Asn
165 170 175
Arg Ser Arg Asp Leu Met Arg Gln Ala Cys Ala Met Leu Pro Ala Glu
180 185 190
Asp Ile Glu Thr Lys Ala Met Met Ala Lys Trp Thr Ala Ala Phe Cys
195 200 205
Arg Val Leu Arg Ile His Phe Gln Pro Glu Val Ser Leu Glu Asp Glu
210 215 220
Met Lys Gly Leu Leu Ser Pro Glu Glu Leu Gln Trp Leu Gln Glu Ser
225 230 235 240
Lys His Arg Pro Cys Ser Val Ile His Val Leu Ser Gln Ile Ile Tyr
245 250 255
Asp Ser Gln Ile Ser Ala Ile Cys Gln Ala Gln Met Cys Asn Asn Leu
260 265 270
Thr Ala Phe Glu Asp Val Leu Gly Gly Cys Glu Arg Leu Leu Arg Ala
275 280 285
Pro Ile Pro Val Ser Tyr Thr Arg His Thr Ala Arg Phe Leu Phe Thr
290 295 300
Trp Leu Thr Leu Leu Pro Phe Ala Leu Tyr Asn Ser Cys Gly Val Trp
305 310 315 320
Thr Leu Pro Val Val Ala Gly Val Ser Ala Val Leu Cys Gly Ile Glu
325 330 335
Glu Ile Gly Val Gln Ile Glu Glu Pro Phe Gly Ile Leu Pro Leu Glu
340 345 350
Ala Ile Cys Gly Arg Ile Gln Ala Asp Val Met Ala Thr Leu Arg Glu
355 360 365
Asp Glu Lys Met Arg Asn Leu Arg Lys Arg Val Ile Gln Thr Arg Thr
370 375 380
Thr Glu Leu Arg Glu Leu Ala Ser Gly Gly Ala Ser Lys
385 390 395
<210> 12
<211> 402
<212> PRT
<213> 耐酸绿藻
<400> 12
Met Lys Val Gly Ile His Val Val Gln Thr Leu Ser Gly His Thr Ile
1 5 10 15
Ser Gly Gln Arg Phe Leu Asn Lys Ala Gly Cys Asn Lys Leu Arg Pro
20 25 30
Ala Asn Lys Ser Ser Phe Arg Ala Gln Ser Thr Ser Ala Leu Asp Glu
35 40 45
Phe Leu His Arg Ile Glu Ile Asn Ile Asp Lys Asp Lys Glu Ala Ala
50 55 60
Arg Ser Glu Arg Arg Gln Ala Phe Thr His Val Asp Trp Lys Arg His
65 70 75 80
Arg Ser Ser Ala Arg Phe Ile Arg His Leu Ser Thr Ile Pro Glu Ser
85 90 95
Trp Val Ile Arg Gly Val Leu Trp His Val Ala Cys Phe Ile Leu Leu
100 105 110
Ala Leu Gly Val Ala Ala Phe Asn Tyr Ser Tyr Ala Cys Lys Gly Leu
115 120 125
Val Pro Pro Tyr Ser Ile Ala Ile Glu Pro Met Gln Leu Thr Ser Phe
130 135 140
Ala Leu Ser Leu Leu Leu Val Phe Arg Thr Asn Ala Ser Tyr Ala Arg
145 150 155 160
Trp Gln Glu Ala Arg Arg Ser Phe Gly Ser Ile Thr Thr Thr Gly Lys
165 170 175
Asp Ile Met Arg Gln Ser Leu Ala Trp Phe Cys Glu Asp Asp Trp Ala
180 185 190
Gly Lys Met Ala Leu Ser Gly Trp Leu Gln Ala Leu Ala Gln Ser Ser
195 200 205
Met Tyr His Leu Gln Asn Glu Gly His Leu Glu Asp Asp Ser Leu Val
210 215 220
Pro Leu Gln Gly Cys Leu Ser Pro Asp Glu Leu Ala Gln Leu Arg Ile
225 230 235 240
Ser Ser His Lys Pro Thr Phe Cys Ile Ala Met Ile Thr Arg Leu Val
245 250 255
Ser Arg Ala Gly Met Pro Thr Glu Leu Leu Leu Arg Met Asp Glu Asn
260 265 270
Ile Ser Thr Leu Val Gln Ala Val Ser Ser Cys Glu Arg Ile Ile Asn
275 280 285
Thr Pro Ile Pro Leu Ser Tyr Thr Arg His Thr Ser Arg Phe Leu Met
290 295 300
Val Trp Leu Thr Cys Leu Pro Phe Ser Leu Trp Gln Tyr Cys Gly Trp
305 310 315 320
Ala Met Val Pro Ile Ala Gly Leu Ile Ser Phe Val Leu Leu Gly Ile
325 330 335
Glu Glu Ile Gly Val Tyr Ile Glu Glu Pro Phe Ser Leu Leu Pro Leu
340 345 350
Glu Ser Leu Cys Asp Lys Leu Thr His Ser Ile Asp Asn Met Met Leu
355 360 365
Glu His Arg Leu Phe Ser Glu Gly Glu Val Ile Thr Pro Lys Gly Ser
370 375 380
Thr Gly Asp Ser Glu Cys Leu Ala Thr Ser Ser Lys Met Gln Arg Tyr
385 390 395 400
Asn Cys
<210> 13
<211> 838
<212> PRT
<213> 耐酸绿藻
<400> 13
Met Phe Phe Arg Lys Ala Cys Ser Gln Pro Ile Gly Arg Lys His Gln
1 5 10 15
Gly Gly His Arg Phe Gln Arg Leu Val Arg Pro Asn Phe Asn Cys Ser
20 25 30
Val Thr Asp Ala Asp Lys Ala Ala Glu Asn Phe Val Leu Ser Arg Ser
35 40 45
Glu Glu Ser Arg Arg Tyr Phe Arg Thr Val Tyr Asp Phe Pro Leu Trp
50 55 60
Gln Lys His Ser Asn Gln Pro Arg Ile Ile Asp Glu Val Arg Lys Asn
65 70 75 80
Ser Arg Thr Asn Ile Leu Lys Ser Ile Leu Pro Ser Thr Ala Trp Cys
85 90 95
Thr Gly Val Ala Ala Leu Leu Thr Ala Tyr Val Gln Ala Tyr Asp Ser
100 105 110
Asn Met Leu Pro Ala Gln Phe Pro Arg Phe Glu Ser Ile Pro Ser Tyr
115 120 125
Ala Ala Ile Val Asn Ile Thr Val Val Ser Leu Ser Leu Leu Leu Val
130 135 140
Phe Arg Thr Asn Ala Ser Tyr Gly Gln Trp Asp Ala Ala Arg Lys Met
145 150 155 160
Lys Gly Leu Leu Val Asn Arg Ser Gln Glu Leu Met Arg Glu Ala Cys
165 170 175
Ser Leu Leu Pro Ala Glu Asp Thr Ala Thr Lys Ile Met Met Gly Arg
180 185 190
Trp Ile Ser Ala Tyr Ala Gly Val Leu Arg Asn Gln Phe Gln Pro Glu
195 200 205
Glu Ser Leu Gly Lys Ile Val Asp Gly Leu Leu Ser Thr Glu Glu Leu
210 215 220
Glu Trp Leu Asn Ser Ser Asn Gln Arg Pro Ser Ser Val Ile Asp Val
225 230 235 240
Leu Ser Gln Ile Ile Ser Ser Ala Ala Ile Pro Ala Pro Asn Gln Ala
245 250 255
Gln Met Asn Asn Asn Leu Ala Ala Leu Arg Gly Val Leu Gly Gly Cys
260 265 270
Gln Arg Leu Leu Lys Asp Pro Ile Pro Met Ser Tyr Thr Arg Asn Ser
275 280 285
Ala Arg Leu Leu Phe Leu Trp Leu Thr Leu Leu Pro Phe Ala Leu Phe
290 295 300
Ser Ser Cys Gly Val Trp Thr Ala Pro Val Val Ala Ala Val Ser Ala
305 310 315 320
Val Leu Cys Ser Ile Glu Glu Ile Gly Val Gln Ile Glu Glu Pro Ser
325 330 335
Thr Thr Gly Met Leu Pro Leu Glu Ala Ile Gly Asp Arg Ile Gln Ala
340 345 350
Asp Val Ile Thr Thr Leu Leu Glu Asp Glu Lys Thr Lys Thr Met Arg
355 360 365
Gly Glu Val Asp Thr Ala Ala Leu Arg Ala Ala Ala Gln Pro Ser Lys
370 375 380
Leu Asp Glu Val Pro Gly Lys Leu Val Leu Leu Ala Glu Gln Val Ala
385 390 395 400
Val Val Gly Gln Ser Ser Asn Asn Gln Gly Thr Gly Gly Ala Gly Gln
405 410 415
Lys Gly Glu Arg Arg Val Leu Leu Ser Lys Leu Glu Thr Glu Thr Lys
420 425 430
Asp Met Lys Asp Asn Leu Gln Arg Leu Lys Gln Arg Ala Thr Glu Leu
435 440 445
Ser Thr Lys Ala Gln Gln Ala Ser Asp Val Ala Arg Lys Glu Arg Ala
450 455 460
Asn Ala Val Leu Lys Leu Gly Glu Met Asp Glu Gln Leu Lys Thr Ser
465 470 475 480
Gln Glu Ala Gly Val Tyr Ser Glu Ile Thr Gln Leu Lys Asp Ser Leu
485 490 495
Thr Thr Arg Glu Ser Asp Val Ala Ser Leu Val Ser Glu Leu Met Leu
500 505 510
Glu Gln Gln Ser Asn Gln Gln Leu Leu Glu Gln Val Ala Leu Leu Gln
515 520 525
Met Gln Leu Ser Glu Thr Glu Asn Met Leu Thr Ser Glu Lys Ser Ile
530 535 540
Gly Glu Gly Leu Ser Gln Gln Leu Gln Ile Thr Gln Val Asp Leu Asn
545 550 555 560
Thr Ser Gln Ser Ser Leu Ala Asp Met Ser Gln Lys Leu Arg Glu Glu
565 570 575
Glu Phe Gln Arg Ala Thr Leu Gln Ser Glu Ile Gln Ala Leu Arg Ser
580 585 590
Asp Leu Asp Arg Val Thr Ala Val Val Glu Thr Arg Gln Lys Glu Ala
595 600 605
Asp Val Tyr Arg Ala Ala Ala Glu Lys Ala Glu Gln Gln Val Val Asp
610 615 620
Ala Ala Ala Leu Arg Gln Ala Met Met Ala Glu Leu Thr Glu Thr Ser
625 630 635 640
Asp Ser Phe Ala Glu Leu Leu Ala Glu Ala Leu Ser Arg Ala Glu Ser
645 650 655
Ala Glu Ala Asp Lys Val Lys Val Glu Ala Glu Val Arg Met Leu Gln
660 665 670
Ser Lys Ile Val Ser Ala Asp Ala Ala Leu Ser Arg Leu Glu Thr Gln
675 680 685
Leu Leu Glu Glu Glu Arg Lys Gly Ile Pro Pro Ser Gly Ser Asn Asp
690 695 700
Met Glu Phe Glu Asp Val Gln Lys Arg Ile Ala Glu Leu Glu Gly Leu
705 710 715 720
Leu Gly Gly Leu Leu Ser Glu Val Glu Gln Ala Thr Val Lys Gln Gly
725 730 735
Arg Thr Arg Leu Thr Gln Asp Thr Ile Glu Glu Val Lys Arg Glu Ala
740 745 750
Glu Leu Arg Ser Ala Arg Val Ala Ala Glu Leu Ala Ala Glu Ser Gly
755 760 765
Ala Arg Ser Glu Ala Glu Asp Asn Leu Arg Val Leu Gln Ser Glu Leu
770 775 780
Leu Gln Leu Lys Glu Ala Ser Gln Thr Ser Arg His Ala Ala Glu Lys
785 790 795 800
Glu Leu Lys Glu Met Gln Leu Leu Ile Ser Glu Val Lys Ala Lys Ala
805 810 815
Ala Glu Thr Ala Ala Phe Val Gly Lys Ala Ala Lys Glu Gly Pro Lys
820 825 830
Lys Gly Lys Ser Ala Tyr
835
<210> 14
<211> 365
<212> PRT
<213> 耐酸绿藻
<400> 14
Met Lys Val Cys Ile His Val Val Gln Thr Leu Ser Gly His Thr Ile
1 5 10 15
Ser Gly Gln Arg Phe Leu Asn Lys Ala Gly Cys Asn Lys Leu Arg Pro
20 25 30
Ala Asn Lys Ser Ser Phe Arg Ala Gln Ser Thr Ser Ala Leu Asp Glu
35 40 45
Phe Leu His Arg Ile Glu Ile Asn Ile Asp Lys Asp Lys Glu Ala Ala
50 55 60
Arg Ser Glu Arg Arg Gln Ala Phe Thr His Val Asp Trp Lys Arg His
65 70 75 80
Arg Ser Ser Ala Arg Phe Ile Arg His Leu Ser Thr Ile Pro Glu Ser
85 90 95
Trp Val Ile Arg Gly Val Leu Trp His Val Ala Cys Phe Ile Leu Leu
100 105 110
Ala Leu Gly Val Ala Ala Phe Asn Tyr Ser Tyr Ala Cys Lys Gly Leu
115 120 125
Val Pro Pro Tyr Ser Ile Ala Ile Glu Pro Met Gln Leu Thr Ser Phe
130 135 140
Ala Leu Ser Leu Leu Leu Val Phe Arg Thr Asn Ala Ser Tyr Ala Arg
145 150 155 160
Trp Gln Glu Ala Arg Arg Ser Phe Gly Ser Ile Thr Thr Thr Gly Lys
165 170 175
Asp Ile Met Arg Gln Ser Leu Ala Trp Phe Cys Glu Asp Asp Trp Ala
180 185 190
Gly Lys Met Ala Leu Ser Gly Trp Leu Gln Ala Leu Ala Gln Ser Ser
195 200 205
Met Tyr His Leu Gln Asn Glu Gly His Leu Glu Asp Asp Ser Leu Val
210 215 220
Pro Leu Gln Gly Cys Leu Asn Pro Asp Glu Leu Ala Gln Leu Arg Ile
225 230 235 240
Ser Ser His Lys Pro Thr Phe Cys Ile Ala Met Ile Thr Arg Leu Val
245 250 255
Ser Arg Ala Gly Met Pro Thr Glu Leu Leu Leu Arg Met Asp Glu Asn
260 265 270
Ile Ser Thr Leu Val Gln Ala Val Ser Ser Cys Glu Arg Ile Ile Asn
275 280 285
Thr Pro Ile Pro Leu Ser Tyr Thr Arg His Thr Ser Arg Phe Leu Met
290 295 300
Val Trp Leu Thr Cys Leu Pro Phe Ser Leu Trp Gln Tyr Cys Gly Trp
305 310 315 320
Ala Met Val Pro Ile Ala Gly Leu Ile Ser Phe Val Leu Leu Gly Ile
325 330 335
Glu Glu Ile Gly Val Tyr Ile Glu Val Ile Ile Phe Phe Leu Leu Tyr
340 345 350
Leu Gly Gln Leu Ser His Asn Cys Ser His Ile Val Lys
355 360 365
<210> 15
<211> 831
<212> PRT
<213> 耐酸绿藻
<400> 15
Met Leu Val Gln Lys Thr Cys Ser Lys Pro Leu Ser Cys Lys Gln Gln
1 5 10 15
Ala Gly Leu Arg Thr Leu Arg Ile Phe Arg Tyr Val Phe Gln Asn Ala
20 25 30
Arg Gln Asp Val Lys Ile Ala Val Ala Lys Val Val Leu Pro Pro Ser
35 40 45
Glu Glu Ala Arg Arg Ser Phe Arg Thr Val Tyr Asp Phe Gln Ser Trp
50 55 60
Arg Lys His Arg Asn Gln Leu Arg Leu Phe Val Gln Val Gly Gln Ile
65 70 75 80
Pro Arg Ser Asn Ile Leu Lys Asn Ile Leu Pro Ser Met Ala Trp Cys
85 90 95
Thr Gly Val Ala Ala Leu Leu Thr Leu Tyr Met Gln Ala Tyr Asp Ser
100 105 110
Asn Leu Leu Pro Glu Pro Phe Pro Ser Phe Ala Ser Asn Thr Ala Ser
115 120 125
Val Thr Phe Val Asn Thr Thr Val Val Ala Leu Ser Leu Leu Leu Val
130 135 140
Phe Arg Thr Asn Ala Ser Tyr Gly Arg Trp Asp Glu Ala Arg Lys Met
145 150 155 160
Lys Gly Leu Leu Val Asn Arg Ser Arg Asp Leu Ile Arg Gln Ala Cys
165 170 175
Ser Met Phe Ser Pro Glu Asp Val Glu Arg Lys Ile Met Met Gly Lys
180 185 190
Trp Val Ser Val Tyr Cys Arg Val Leu Arg Ile His Phe Gln Ala Glu
195 200 205
Val Ser Leu Glu Glu Glu Met Asn Gly Ile Leu Ser Met Glu Glu Val
210 215 220
Glu Trp Leu Gln Ser Ser Thr His Arg Pro Cys Thr Val Ile His Val
225 230 235 240
Leu Ser Gln Ile Ile Tyr Ser Ala Pro Ile Ser Ala Phe Cys Gln Ala
245 250 255
Gln Met Cys Asn Asn Leu Thr Ala Leu Glu Asp Val Leu Gly Gly Cys
260 265 270
Glu Arg Ile Leu Arg Ala Pro Ile Pro Val Ser Tyr Thr Arg Asn Thr
275 280 285
Ala Arg Phe Leu Phe Val Trp Leu Thr Leu Leu Pro Phe Ala Leu Phe
290 295 300
Ser Ser Cys Gly Val Trp Thr Ala Pro Val Val Ala Gly Ile Ala Ala
305 310 315 320
Val Leu Cys Gly Ile Glu Glu Ile Gly Val Gln Val Glu Glu Pro Phe
325 330 335
Gly Ile Leu Pro Leu Ala Ala Ile Gly Asp Arg Ile Gln Ala Asp Val
340 345 350
Ile Ser Thr Leu Leu Glu Asp Gly Arg Thr Arg Thr Met Arg Ser Glu
355 360 365
Val Asn Thr Ala Ala Leu Arg Ala Met Ala Gln Pro Pro Asn Gln Glu
370 375 380
Gly Val Leu Gly Ser Val Asp Arg Gln Ser Thr Asn Leu Ala Asn Ser
385 390 395 400
Gln Glu Ser Thr Thr Gly Ser Asn Pro Met Gly Gln Arg Val Val Leu
405 410 415
Leu Ser Lys Leu Glu Thr Glu Thr Lys Asp Met Lys Asp Asn Leu Asn
420 425 430
Lys Leu Lys Gln Arg Ala Thr Glu Leu Ser Met Lys Ala Gln Leu Ala
435 440 445
Ser Glu Ala Ala Gln Arg Glu Arg Ala Ser Ala Val Gln Lys Leu Gly
450 455 460
Ile Met Asn Glu Gln Leu Lys Gly Phe Lys Gly Ala Glu Val Tyr Ser
465 470 475 480
Glu Ile Ala Gln Leu Lys Asp Thr Leu Thr Ser Arg Glu Ser Asp Val
485 490 495
Ala Thr Val Val Ser Glu Met Gln Gln Gln Gln Leu Ser Ser Gln Gln
500 505 510
Leu Phe Glu Gln Leu Ala His Leu Gln Thr Gln Leu Ser Glu Ala Glu
515 520 525
Ala Leu Leu Ser Thr Glu Lys Ser Met Gly Gln Glu Leu Ser Gln Gln
530 535 540
Leu Gln Leu Thr Gln Met Gly Leu Lys Thr Asn Gln Ala Ser Leu Leu
545 550 555 560
Asp Val Ser His Lys Leu Gln Lys Glu Glu Leu Gln Arg Ser Ala Phe
565 570 575
Gln Thr Glu Ile Gln Ala Leu Arg Arg Asp Leu Asp Arg Ile Thr Thr
580 585 590
Ala Met Glu Ser Lys Arg Leu Glu Thr Glu Ala Phe Trp Ala Ala Ala
595 600 605
Glu Ser Ala Glu Gln His Val Ala Asp Ser Ala Ala Leu Gln Ser Ala
610 615 620
Met Met Ala Glu Leu Thr Glu Thr Ser Asp Ala Phe Ala Glu Arg Leu
625 630 635 640
Ala Ala Ala Leu Ser Arg Ala Glu Thr Ala Glu Thr Asp Lys Phe Arg
645 650 655
Ala Glu Gln Glu Val Lys Met Leu Gln Ser Lys Ile Ala Asp Thr Glu
660 665 670
Thr Met Leu Arg Lys Leu Glu Ala Lys Ala Ala Glu Leu Glu Arg Lys
675 680 685
Gly Ser Ala Pro Ser Glu Ser Thr Ala Leu Glu Phe Arg Asp Ala Gln
690 695 700
Trp Lys Ile Ala Glu Leu Glu Gly Leu Leu Gly Glu Val Leu Leu Glu
705 710 715 720
Val Lys His Ala Ala Ser Asn Asn Ala Thr Glu Tyr Ser Thr Gln Asp
725 730 735
Lys Ile His Glu Val Gln Arg Glu Ala Asp Arg Arg Ser Ile Arg Val
740 745 750
Ala Ala Glu Ile Ala Ala Glu Arg Gly Ala Arg Ser Glu Ala Glu Asp
755 760 765
Asn Leu Arg Ile Leu Gln Arg Glu Leu Leu Leu Leu Lys Glu Asp Ser
770 775 780
Leu Ser Ser Arg Asn Ala Ala Glu Arg Glu Leu Lys Glu Met Gln Leu
785 790 795 800
Leu Ile Cys Glu Val Lys Ala Lys Ala Ala Ser Thr Ala Thr Phe Val
805 810 815
Gly Thr Ala Ala Ala Glu Gly Pro Arg Arg Gly Gln Asn Thr Tyr
820 825 830
<210> 16
<211> 451
<212> PRT
<213> 小球藻
<400> 16
Met Thr Ala Ser Asp Ala Pro Ser Ala Ser Arg Ser Gly Gly His Ala
1 5 10 15
Arg Leu Val Gln Thr Ala Ala Ser Pro Arg Gly Asp Gly Ser Phe Ala
20 25 30
Val Gly Ser Thr Asp Leu Glu Gln Val Ala Glu Gln Ala Val Val Asp
35 40 45
Ala Val Ala Gln Thr Ala Gly Asp Arg Ala Thr Ser Ser Ser Ala Ala
50 55 60
Ala Gly Ser Arg Glu Val Asp Pro Ile Lys Glu Gly Ser Arg Lys Tyr
65 70 75 80
Arg Arg Thr Val Tyr Asp Phe Glu Asn Trp Arg Gln His Arg Ser Thr
85 90 95
Lys Arg Tyr Met Arg His Ala Lys Gly Leu Leu Gly Ser Arg Ile Phe
100 105 110
Arg Gly Leu Ala Ser Pro Leu Leu Tyr Ile Leu Ala Val Ser Ala Ser
115 120 125
Val Ala Val Trp Asn Thr Leu Val Glu Thr Gly Leu Ala Pro Asp Val
130 135 140
Leu Pro Glu Leu His Met Ser Asn Asn Gly Pro Phe Gly Leu Thr Ser
145 150 155 160
Phe Ala Leu Ser Leu Leu Leu Val Thr Asn Ala Ser Tyr Ala Arg Trp
165 170 175
Leu Asp Ala Arg Lys Ala Trp Gly Met Leu Val Asn Arg Ser Arg Asp
180 185 190
Ile Thr Arg Gln Ala Leu Thr Cys Phe Pro Ala Ala Asp Arg Pro Leu
195 200 205
Leu Asp Met Leu Cys Arg Trp Thr Ala Ala Tyr Ser Arg Ala Leu Met
210 215 220
Cys His Val Arg Glu Asp Ser Asp Leu Glu Ala Glu Leu Arg Lys Val
225 230 235 240
Leu Pro Ala His Glu Val Glu Ala Val Val Leu Ala Lys His Arg Pro
245 250 255
Asn Tyr Cys Leu Gln Val Met Ser Glu Ile Val His Ser Ala His Leu
260 265 270
Ser Ala Leu Pro Leu Pro Val Ala Arg Gly Ala Val Val Ala Ser Gly
275 280 285
Ala Pro Ala Leu Ala Ala Ala Gly Trp Glu Gly Thr Arg Tyr Arg Met
290 295 300
Asp Glu Asn Leu Thr Ala Met Glu Asp Ile Leu Gly Ala Cys Glu Arg
305 310 315 320
Ile Leu Arg Ala Pro Ile Pro Leu Ser Tyr Thr Arg His Thr Ser Arg
325 330 335
Phe Met Met Ile Trp Leu Thr Leu Leu Pro Phe Ser Leu Trp Asp Asn
340 345 350
Cys Gly Trp Ala Ser Val Pro Leu Cys Gly Ile Ile Ala Phe Leu Leu
355 360 365
Leu Gly Ile Glu Glu Ile Gly Val Ser Ile Glu Glu Pro Phe Ser Ile
370 375 380
Leu Pro Leu Glu Val Ile Cys Asp Ile Ile Glu Ala Asn Val Arg Glu
385 390 395 400
Leu Arg Ser Ile His Ala Gly Ser His Ala Glu Asp Gly Ser Gln Pro
405 410 415
Arg Arg Ala Pro Ala Ala Arg Asp Leu Val Gly Arg Ala Val Ala Glu
420 425 430
Ala Glu Gln Glu Glu Ala Ala Ala Ala Ala Gly Thr Val Ala Ala Ala
435 440 445
Ala Gly Ala
450
<210> 17
<211> 456
<212> PRT
<213> 小球藻
<400> 17
Met Ala Ser Arg Leu His Gly Ser Arg His Gln Gly Ala Leu Leu Gly
1 5 10 15
Leu Gln Gln Pro Ala Gly Leu Val Pro Arg Thr Arg Ser Leu Asn Arg
20 25 30
Gly Arg Leu Thr Gln Leu Arg Val Ala Ala Ala Ala Ser Ser Pro Pro
35 40 45
Leu Pro Ala Lys Leu Ser Gly Asp Asp Leu Lys Glu Ala Glu Leu Lys
50 55 60
Lys Leu Arg Thr Val Ala Asp Phe Glu Phe Trp Lys Arg His Arg Ser
65 70 75 80
Ser Ser Arg Tyr Trp Arg His Val Leu Gly Ile Phe Asp Ser Arg Thr
85 90 95
Phe Ser Trp Val Ala Ala Pro Leu Ser Tyr Val Met Leu Leu Thr Thr
100 105 110
Gly Val Cys Leu Tyr Tyr Thr Leu Ala Glu Ala Gly Ile Val Pro Glu
115 120 125
Val Ile Pro Glu Ile Ser Ala Ser Ala Ala Ala Pro Phe Gly Leu Thr
130 135 140
Ser Phe Ala Leu Ser Thr Leu Leu Val Leu Arg Thr Asn Thr Ser Tyr
145 150 155 160
Gln Arg Trp Asp Glu Ala Arg Lys Met Trp Gly Leu Ile Val Asn Arg
165 170 175
Thr Arg Asp Ile Ser Arg Gln Ala Val Gly Tyr Ile Pro Pro His Gln
180 185 190
Ala Glu Leu Gln Asp Met Phe Cys Arg Trp Leu Val Ala Tyr Cys Arg
195 200 205
Ser Leu Met Cys His Leu Arg Ala Gly Glu Asp Leu Glu Ala Glu Leu
210 215 220
Lys Gly Lys Leu Thr Asp Ile Glu Leu Lys Ala Leu Leu Ala Ser Thr
225 230 235 240
His Arg Pro Asn Tyr Thr Cys Gln Val Leu Thr Ala Ile Ile Arg Ala
245 250 255
Ala Gln Leu Pro Gly Gly Lys Val Asp Met Asn Asp Ser Phe Ala Asn
260 265 270
Val Lys Ala Ser Ala Ala Phe Arg Met Asp Glu Asn Leu Thr Gln Tyr
275 280 285
Ala Asp Val Thr Gly Gly Cys Glu Arg Ile Leu Arg Thr Pro Val Pro
290 295 300
Leu Ser Tyr Ser Arg His Asn Ser Arg Phe Leu Ile Ile Trp Leu Thr
305 310 315 320
Leu Leu Pro Phe Thr Leu Trp Asp Gln Cys His Trp Phe Thr Leu Pro
325 330 335
Val Thr Gly Leu Val Ala Phe Leu Leu Leu Gly Ile Lys Glu Ile Gly
340 345 350
Val Val Val Glu Glu Pro Phe Ser Ile Leu Pro Leu Glu Arg Ile Cys
355 360 365
Asp Thr Ile Glu Ala Asn Val Trp Glu Ile His Ser Ile His Ser Ala
370 375 380
Lys Ala Met Lys Glu Gln Ala Glu Ala Arg Ala Ala Gly Leu Pro Ser
385 390 395 400
Asp Leu Met Glu Ala Ser Asp Leu Val Ser Val Val Ser Glu Leu Ala
405 410 415
Leu Ala Ala Gly Thr Gly Gly Thr Gly Thr Thr Ala Gly Asn Gly Asn
420 425 430
Gly Ala Gly Asn Gly Asn Gly Ser Ala Ala Ala Ala Val Val Ala Ala
435 440 445
Asn Gly Ala Val Val Val Lys Val
450 455
<210> 18
<211> 480
<212> PRT
<213> 小球藻
<400> 18
Met Leu Ala His Ser Arg Pro Gly Ala Gly Ser Arg Pro Gly Asn Gly
1 5 10 15
Ala Ala Lys Ala Gly Ala Gly Gly Arg Ser Ala Pro Pro Leu Gln Pro
20 25 30
Ala Pro Ala Leu Thr Arg Arg Ala Leu Val His Pro Val Pro Ser Pro
35 40 45
Arg Pro Thr Pro Gly Leu Val Arg Gln Arg Pro Gln Arg Gln Arg Pro
50 55 60
Gln Glu Leu Pro Gly Ala Thr Pro Glu Gln Gln Gln Gln Phe Pro Gly
65 70 75 80
Ser Glu Leu Asp Ala Ala Ala Lys Ala Glu Ala Ala Ala Ala Lys Leu
85 90 95
Gln Arg Tyr Pro Trp His Ser Asp Glu Trp Lys Glu Glu Tyr Arg Lys
100 105 110
Gln Val Arg Val Thr Phe Asp Phe Glu Arg Trp Arg Thr His Arg Ser
115 120 125
Ser Ser Arg Phe Trp Arg His Met Ala Gly Leu Ala Gln Ser Asn Thr
130 135 140
Ala Gln Gly Leu Ala Gln Pro Leu Ala Tyr Val Met Ala Val Ser Leu
145 150 155 160
Gly Val Ala Ala Tyr His Val Ala Ala Ala Ala Gly Trp Leu Pro Leu
165 170 175
Trp Pro Val Leu Lys Leu Ala Ala Asn Ala Pro Phe Gly Leu Thr Ser
180 185 190
Phe Ala Leu Ser Leu Leu Leu Val Phe Arg Thr Asn Ser Ser Tyr Gly
195 200 205
Arg Trp Asp Glu Ala Arg Lys Met Trp Gly Leu Val Val Asn Arg Ser
210 215 220
Arg Asp Leu Thr Arg Gln Ala Leu Gly Tyr Ile Pro Ala His Gln Ala
225 230 235 240
Glu Leu Gln Ser Met Leu Cys Arg Trp Val Val Ala Tyr Ser Arg Cys
245 250 255
Leu Met Cys His Leu Arg Glu Gly Glu Asp Leu Glu Ala Glu Leu Arg
260 265 270
Gly Val Leu Leu Pro Glu Glu Val Ala Glu Leu Leu Ala Ala Glu His
275 280 285
Arg Pro Asn Tyr Cys Cys Gln Val Leu Thr Glu Val Leu Arg Glu Ala
290 295 300
Gln Leu Pro Ala Ala Val Thr Ser Pro Thr Asp Ser Thr Gly Cys Val
305 310 315 320
Pro Ala Gly Ala Ala Tyr Arg Met Asp Glu Asn Leu Thr Val Phe Glu
325 330 335
Asp Val Thr Gly Gly Cys Glu Arg Leu Leu Arg Thr Pro Ile Pro Leu
340 345 350
Ala Tyr Thr Arg His Thr Ser Arg Phe Met Met Ala Trp Leu Thr Ile
355 360 365
Leu Pro Phe Ala Leu Trp Asp Thr Cys Gly Trp Ala Met Leu Pro Val
370 375 380
Met Ala Ile Val Ala Phe Val Leu Leu Gly Ile Glu Glu Ile Gly Val
385 390 395 400
Ser Ile Glu Glu Pro Phe Ser Ile Leu Pro Leu Glu Thr Ile Cys Arg
405 410 415
Thr Ile Glu Ser Asn Val Arg Glu Ser Val Gly Ala His Cys Gln Arg
420 425 430
Gln Ala Arg Arg Ala Gln Gln Ala Gln His Gly Gly Val Pro Gly Asp
435 440 445
Leu Pro Lys Arg His Ala His Ser Asn Gly Asn Asn Ala Val Pro Ala
450 455 460
Leu Leu Phe Met Pro Pro Gln Pro Val Ala Ser Ala Gly Gln Gln His
465 470 475 480
<210> 19
<211> 318
<212> PRT
<213> 小球藻
<400> 19
Val Phe Thr Phe Glu Arg Trp Asn Lys His Arg Asn Ser Met Arg Tyr
1 5 10 15
Ala Lys His Leu Ala His Met Phe Thr Ser Arg Val Phe Arg Gln Leu
20 25 30
Leu Gly Pro Val Leu Ala Val Met Thr Ile Ala Leu Ala Val Gly Val
35 40 45
Tyr Glu Thr Leu Val Gly Ala Gly Ala Leu Pro Gly His Trp Pro His
50 55 60
Val Thr Leu Ala Leu Gly Gln Gly Phe Asn Leu Thr Ala Phe Ala Leu
65 70 75 80
Ser Leu Leu Leu Val Phe Arg Thr Asn Ser Ser Tyr Asp Arg Trp Trp
85 90 95
Glu Ala Arg Lys Leu Trp Gly Gly Val Val Asn Arg Cys Arg Asp Ile
100 105 110
Val Arg Gln Val Gly Pro Gln Gly Leu Val Phe Phe Arg Asp Glu Asp
115 120 125
Ala His Leu Lys Glu Leu Leu Ala Arg Trp Thr Met Ala Phe Pro Arg
130 135 140
Val Leu Met Cys His Leu Arg Glu Asp Met Asp Val Gly Lys Glu Val
145 150 155 160
Ala His Ile Leu Thr Ala His Glu Val Ala Val Met Cys Ala Ala Ala
165 170 175
His Arg Pro Asn Phe Val Leu Gln Val Met Ala Glu Thr Val Arg Ala
180 185 190
Ala Arg Pro Asn Glu Leu Cys Arg Met Arg Met Asp Asp Asn Leu Thr
195 200 205
Phe Phe Glu Asp Ala Met Gly Ser Cys Glu Arg Ile Leu Arg Thr Pro
210 215 220
Ile Pro Leu Ser Tyr Thr Arg His Thr Ser Arg Phe Leu Leu Val Trp
225 230 235 240
Leu Ile Leu Leu Pro Phe Thr Leu Trp Ala Ala Tyr Ser Trp Phe Ser
245 250 255
Ile Leu Leu Ser Gly Ile Phe Ala Phe Leu Met Phe Gly Ile Asp Glu
260 265 270
Ile Gly Val Gln Ile Glu Glu Pro Phe Gly Trp Val Ala Ala Thr Tyr
275 280 285
Thr Ala Trp His Leu Ala Cys Glu Lys Pro Gly Cys Cys Cys Trp Val
290 295 300
Val Leu Cys Gly Gly Val Arg Ala Ser Ala Ala His Gln Arg
305 310 315
<210> 20
<211> 433
<212> PRT
<213> 小球藻
<400> 20
Met Ala Ile Leu Leu Gly Ala Arg Val Gln Pro Val Ala Val Ala Ala
1 5 10 15
Thr Pro Ala Ala Lys Arg Gln Ala Arg Gly Ser Thr Leu Arg His Cys
20 25 30
Gln Pro Ala Gly Leu Ala Ala Leu Arg Gln Arg Cys Ala Thr Ser Arg
35 40 45
Cys Ser Arg Ala Ala Leu Arg Thr Leu Ala Val Ala Ser Thr Asp Lys
50 55 60
Thr Glu Pro Glu His Pro Tyr Phe Ser Asp Asp Trp Lys Glu Tyr Asn
65 70 75 80
Arg Arg Phe Gln Arg Pro Val Phe Asp Phe Glu Arg Trp Lys Thr His
85 90 95
Arg Ser Ser Ser Arg Tyr Leu Arg His Val Leu Gly Ile Phe Asp Ser
100 105 110
Lys Ile Val Gln Gly Leu Ala Lys Pro Leu Ala Tyr Val Met Thr Leu
115 120 125
Ala Thr Gly Val Ala Leu Tyr His Thr Leu Ala Glu Ala Gly Tyr Leu
130 135 140
Thr Asp Val Pro Asp Leu Lys Ala Thr Asn Ala Pro Phe Gly Leu Thr
145 150 155 160
Ser Phe Ala Leu Ser Leu Leu Leu Val Phe Arg Thr Asn Thr Ser Tyr
165 170 175
Gln Arg Trp Asp Glu Ala Arg Lys Met Trp Gly Ser Met Val Asn Arg
180 185 190
Ser Arg Asp Phe Thr Arg Gln Ala Leu Gly Tyr Val Pro Glu Ser Gln
195 200 205
Pro Glu Leu Arg Ser Met Leu Val Arg Trp Ser Ile Ala Tyr Pro Arg
210 215 220
Ala Leu Met Cys His Leu Arg Pro Gly Glu Asn Ile Glu Glu Glu Val
225 230 235 240
Lys Asp Ile Leu Lys Pro Glu Glu Val Lys Ala Leu Ala Ala Ser Thr
245 250 255
His Arg Pro Asn Tyr Cys Met Gln Val Leu Thr Ala Cys Leu Lys Gln
260 265 270
Ala Gln Leu Pro Ala Ala Val Thr Ser Asn Arg Asp Ser Tyr Gly Ala
275 280 285
Val Pro Ala Gly Ala Ala Tyr Arg Met Asp Glu Asn Leu Thr Val Tyr
290 295 300
Ser Asp Val Thr Gly Gly Cys Glu Arg Ile Leu Arg Thr Pro Val Pro
305 310 315 320
Leu Ser Tyr Thr Arg His Thr Ser Arg Phe Met Met Ile Trp Leu Thr
325 330 335
Leu Leu Pro Phe Thr Leu Trp Asp Asn Cys Gly Trp Ala Met Leu Pro
340 345 350
Ile Thr Phe Ile Val Ser Phe Leu Leu Leu Gly Ile Glu Glu Ile Gly
355 360 365
Val Ser Ile Glu Glu Pro Phe Thr Ile Leu Pro Leu Glu Thr Ile Ala
370 375 380
Arg Thr Ile Glu Ala Asn Leu Arg Glu Leu Glu Ala Thr His Gly Pro
385 390 395 400
Ala Thr Leu Gly Lys Ala Gly Pro Gly Gln Val Asn Ala Thr Glu Leu
405 410 415
Leu Tyr Asp Met Ile Pro Ser Ala Ala Asn Gly Arg Ile Ser Ile Asn
420 425 430
Ala
<210> 21
<211> 503
<212> PRT
<213> 小球藻
<400> 21
Met Gln Leu Gly Ala Gly Arg Gln Val Ser Ala Ala Pro Val Ala Gly
1 5 10 15
Pro Ala Ser Arg Leu Pro Cys Gly Leu Gly Leu Ala Ser Pro Arg His
20 25 30
Pro Trp Pro Ala Ser Arg Gly Pro Leu Thr His His Gln Arg Arg Arg
35 40 45
Gln Leu Arg Val Ala Val Ala Gly Gly Pro Gly Gln Gln Pro Glu Glu
50 55 60
Ala Pro Glu Arg Pro Ala Phe Pro Ala Ala Ala Ala Ala Val Arg Arg
65 70 75 80
Gln Gln Ile Gln Arg Glu Arg Ala Ala Ile Arg Asn Arg Ile Glu Asn
85 90 95
Leu Val Ala Thr Asp Phe Asp Glu Glu Asp Ala Ala Leu Ile Asp Leu
100 105 110
Val Gly Asp Glu Phe Lys Glu Ala Asp Arg Lys Asn Leu Arg Thr Val
115 120 125
Phe Asp Phe Asp Arg Trp Lys Lys His Arg Ser Gly Ser Arg Tyr Met
130 135 140
Arg His Ala Arg Ala Thr Phe Thr Ser Arg Ile Thr Gln Gly Leu Ala
145 150 155 160
Ala Pro Leu Leu Tyr Val Thr Gly Leu Ser Ile Ala Val Ala Ser Trp
165 170 175
His Thr Ala Ala Glu Val Arg Trp Ala Gly Pro Met Gly Ile Leu Ser
180 185 190
Pro Ile Pro Glu Leu Lys Ile Asp Thr Thr Phe Ser Leu Thr Ser Phe
195 200 205
Ala Leu Ser Leu Leu Leu Ala Tyr Arg Thr Asn Ala Gly Tyr Gly Arg
210 215 220
Trp Asp Glu Ala Arg Lys Met Trp Gly Leu Val Val Asn Arg Ser Arg
225 230 235 240
Asp Met Thr Arg Gln Val Gly Ala Arg Leu Ala Gly Ala Gly Gln Cys
245 250 255
Val His Asn Ile Phe Ile Thr Val Cys Thr Cys Ser Arg Ser Leu Met
260 265 270
Cys His Leu Arg Gly Gly Glu Asp Ile Glu Ser Glu Leu Ala Gly Val
275 280 285
Leu Thr Pro Arg Glu Leu Asp Ala Leu Lys Ala Ser Ala His Arg Pro
290 295 300
Asn Tyr Cys Val Gln Val Arg Gly Arg Arg Val Ala Arg Gly Ser Ala
305 310 315 320
Val Gly Gly Ser Leu Pro Pro Leu Pro Pro Ala Ala Ala Ser Pro Gln
325 330 335
Asp Val Thr Gly Gly Cys Glu Arg Ile Leu Arg Thr Pro Ile Pro Leu
340 345 350
Ser Tyr Thr Arg His Thr Ser Arg Phe Met Met Ile Trp Leu Thr Val
355 360 365
Met Pro Phe Thr Leu Trp Asp Ala Ala Gly Trp Ala Met Val Pro Leu
370 375 380
Ala Met Thr Val Ala Phe Leu Leu Leu Gly Ile Glu Glu Ile Gly Val
385 390 395 400
Met Ile Glu Glu Pro Phe Ser Val Leu Pro Leu Glu Val Ile Ser Arg
405 410 415
Thr Ile Glu Gly Asn Val Arg Glu Leu Glu Arg Ala His Gly Leu Glu
420 425 430
Ala Ala Ala Met Lys Glu Arg Glu Ala Ala Leu Ala Ala Ala Arg Asp
435 440 445
Gly Gly Gly Ala Ala Ala Pro Ala Ala Pro Ala Asp Gly Gln Val Asp
450 455 460
Ala Leu Asp Leu Val Leu Ser Ile Leu Pro Glu Glu Glu Leu Arg Gln
465 470 475 480
Pro Trp Gly Pro Gly Leu Ala Met Leu Ser Gly Thr Ala Gly Pro Ala
485 490 495
Thr Val Pro Ala Gly Asn Ser
500
<210> 22
<211> 444
<212> PRT
<213> 小球藻
<400> 22
Met Gly Ala Ala Ala Pro Pro Ser Ala Ala Leu Ala Arg Pro Ala Ala
1 5 10 15
Gly Gln Arg Pro Leu Ala Ala Gln Leu Arg Gln Arg Gln Pro Gly Leu
20 25 30
Ala Ala Leu Thr Gln Arg His His Ala Ala Ala Cys Arg Cys Asn Arg
35 40 45
Cys Gly Arg Gly Val Ala Leu Arg Thr Ser Ala Val Ala Asn Thr Asp
50 55 60
Lys Arg Glu Gln Pro His Pro Tyr Phe Thr Asp Glu Trp Lys Glu Tyr
65 70 75 80
Asn Arg Gln Phe Gln Arg Pro Val Phe Asp Phe Glu Arg Trp Lys Thr
85 90 95
His Arg Ser Ser Ser Arg Tyr Leu Arg His Val Leu Gly Met Gly Asp
100 105 110
Ser Lys Ile Val Gln Gly Leu Ala Lys Pro Leu Ala Tyr Val Met Thr
115 120 125
Leu Ala Thr Gly Val Ala Leu Tyr His Thr Leu Ala Glu Ala Gly Tyr
130 135 140
Leu Thr Asp Val Pro Asp Leu Lys Ala Thr Asn Ala Pro Phe Gly Leu
145 150 155 160
Thr Ser Phe Ala Leu Ser Leu Leu Leu Val Phe Arg Thr Asn Thr Ser
165 170 175
Tyr Gln Arg Trp Asp Glu Ala Arg Lys Met Trp Gly Ser Met Val Asn
180 185 190
Arg Ser Arg Asp Phe Thr Arg Gln Ala Leu Gly Tyr Val Pro Tyr Ser
195 200 205
Gln Pro Glu Leu Arg Ser Met Leu Val Arg Trp Ser Ile Ala Tyr Pro
210 215 220
Arg Ala Leu Met Cys His Leu Arg Pro Gly Glu Asn Ile Glu Glu Glu
225 230 235 240
Val Lys Asp Ile Leu Lys Pro Glu Glu Val Lys Ala Leu Ala Ala Ser
245 250 255
Thr His Arg Pro Asn Tyr Cys Met Gln Val Leu Thr Ala Cys Ile Lys
260 265 270
Gln Ala Gln Leu Pro Ala Ala Val Thr Ser Asn Arg Asp Ser Tyr Gly
275 280 285
Ala Val Pro Ala Gly Ala Ala Tyr Arg Met Asp Glu Asn Leu Thr Val
290 295 300
Tyr Ser Asp Val Thr Gly Gly Cys Glu Arg Ile Leu Arg Thr Pro Val
305 310 315 320
Pro Leu Ser Tyr Thr Arg His Thr Ser Arg Phe Met Met Ile Trp Leu
325 330 335
Thr Leu Leu Pro Phe Thr Leu Trp Asp Asn Cys Gly Trp Ala Met Leu
340 345 350
Pro Ile Thr Phe Ile Val Ser Phe Leu Leu Leu Gly Ile Glu Glu Ile
355 360 365
Gly Val Ser Ile Glu Glu Pro Phe Thr Ile Leu Pro Leu Glu Thr Ile
370 375 380
Ala Arg Thr Ile Glu Ala Asn Leu Arg Glu Leu Glu Ala Thr His Gly
385 390 395 400
Pro Ala Thr Leu Gly Lys Ala Pro Glu Gly Ala Met Asn Ala Thr Glu
405 410 415
Leu Leu Tyr Glu Val Ile Pro Ser Ala Ala Pro Ala Asp Thr Val Asn
420 425 430
Ala Asn Ser Asn Gly Ala Gln Ile Ala Val Lys Ala
435 440
<210> 23
<211> 273
<212> PRT
<213> 小球藻
<400> 23
Ser Thr Phe Thr Phe Arg Arg Trp Ala Phe His Arg Ser Thr Ser Arg
1 5 10 15
Tyr Val Arg His Met Ser Gly Ile Phe Gln Ser Arg Ile Val Arg Gly
20 25 30
Leu Ala Gln Pro Leu Leu Ser Ser Cys Ala Thr Ala Thr Ile Val Cys
35 40 45
Val Tyr Glu Gln Ala Leu Gln Asp Gly Trp Leu Pro Ser Phe Leu Pro
50 55 60
Thr Phe Ile Met Pro Ser Leu Pro Phe Asp Ile Thr Ala Ser Ser Leu
65 70 75 80
Gly Leu Leu Leu Val Phe Arg Thr Asn Ser Ser Tyr Asp Arg Trp Gln
85 90 95
Gln Ala Val Ser Ala Trp Gly Asp Ile Glu Thr Arg Ala Arg Asp Thr
100 105 110
Leu Arg Gln Leu Leu Ala Ala Ala Ser His Ser Ser Ala Gly His Gly
115 120 125
Gly Ala Arg Gln Ala Ala Leu Trp Leu Val Ala Phe Ser Arg Ser Leu
130 135 140
Lys Ala Gln Leu Thr Glu Asp Ser Asp Val Gln Ala Glu Leu Arg Glu
145 150 155 160
Val Leu Thr Gly Gln Glu Leu Ala Leu Leu Leu Gly Ala Gln His Arg
165 170 175
Pro Ser Phe Ala Leu Ala Val Leu Ser Glu Leu Ala Glu Ala Ala Pro
180 185 190
Leu Arg Asp Ala Gln Arg Val Arg Val Asp Glu Asn Leu Ser Cys Phe
195 200 205
Gln Asp Ala Ala Gly Arg Cys Glu Arg Ile Leu Arg Thr Pro Ile Pro
210 215 220
Leu Ser Tyr Thr Arg His Ser Ser Arg Phe Met Val Ile Trp Leu Ser
225 230 235 240
Ala Leu Pro Leu Gly Leu Trp Ser Gln Cys Gly Phe Gly Thr Ile Pro
245 250 255
Leu Thr Val Ile Ser Pro Ser Cys Cys Trp Ala Leu Arg Arg Leu Gly
260 265 270
Val
<210> 24
<211> 668
<212> PRT
<213> 莱茵衣藻
<400> 24
Met Gln Cys Gln Leu Lys His Gly Ala Arg Pro Gln Ser Gln Arg Pro
1 5 10 15
Asn Trp Leu Pro Ala Arg Ala Ala Thr Leu Arg Pro Ala Val Gln His
20 25 30
Gly Val Arg Arg Gly Leu Thr Leu Gly Val Lys Ala Ala Ala Ala Pro
35 40 45
Leu Glu Asp Lys Lys Met Pro Ala Asp Met Thr Thr Arg Gln Tyr Arg
50 55 60
Arg Val Val Tyr Asp Phe Ala Leu Trp Ala Lys His Arg Asp Val Asn
65 70 75 80
Arg Tyr Leu Tyr Asn Leu Arg Thr Ile Pro Gly Ser Arg Ile Ile Arg
85 90 95
Gln Leu Ser Gln Pro Met Gly Val Val Leu Ala Trp Ala Ala Leu Phe
100 105 110
Gly Phe Tyr Glu Thr Cys Leu Glu Ala Gly Val Leu Pro Ser Tyr Leu
115 120 125
Pro Lys Met Thr Leu Met Ser Ala Glu Pro Gln Gly Leu Thr Ser Phe
130 135 140
Ala Leu Ser Leu Leu Leu Val Phe Arg Thr Asn Ser Ser Tyr Gly Arg
145 150 155 160
Phe Asp Glu Ala Arg Lys Ile Trp Gly Gly Ile Leu Asn Arg Ala Arg
165 170 175
Asn Ile Ala Asn Gln Ala Val Thr Phe Ile Pro Ala Glu Asp Gln Ala
180 185 190
Gly Arg Glu Ala Val Gly Lys Trp Thr Val Gly Phe Thr Arg Ala Leu
195 200 205
Gln Ala His Leu Gln Glu Asp Ile Asp Leu Arg Lys Glu Leu Glu Lys
210 215 220
Ala Thr Pro Arg Trp Ser Lys Glu Glu Ile Asp Met Leu Val Asn Ala
225 230 235 240
Gln His Arg Pro Ile Lys Ala Ile Ser Val Leu Ser Glu Leu Thr Arg
245 250 255
Gln Leu Ser Ile Thr Gln Phe Gln Ala Leu Gln Met Gln Glu Asn Cys
260 265 270
Thr Phe Phe Tyr Asp Ala Leu Gly Gly Cys Glu Arg Leu Leu Arg Thr
275 280 285
Pro Ile Pro Val Ser Tyr Thr Arg His Thr Ala Arg Phe Leu Thr Ile
290 295 300
Trp Leu Ala Met Leu Pro Leu Gly Leu Trp Glu Arg Tyr His Trp Ser
305 310 315 320
Met Leu Pro Val Ile Ala Leu Ile Gly Phe Leu Leu Leu Gly Ile Asp
325 330 335
Glu Ile Gly Ile Ser Ile Glu Glu Pro Phe Gly Ile Leu Pro Leu Asp
340 345 350
Ala Ile Cys Gly Arg Ala Gln Thr Asp Val Asn Ser Leu Leu Lys Glu
355 360 365
Asp Pro Ala Val Met Lys Tyr Val Asp Asp Val Arg Ser Gly Arg Val
370 375 380
Lys Ser Pro Pro Pro Leu Pro Pro Ala Pro Ala Ala Pro Ala Ala Ala
385 390 395 400
Ala Ala Ala Ala Ala Ala Ala Ala Arg Ser Val Ser Pro Gln Pro Asp
405 410 415
Val Ala Lys Thr Leu Gly Ser Leu Phe Thr Asn Val Arg Ala Gly Val
420 425 430
Gly Ala Val Ala Pro Gly Ala Pro Leu Met Pro Gln Ala Pro Val Arg
435 440 445
Ser Pro Ser Pro Thr Arg Ser Val Ser Pro Ser Phe Pro Arg Ala Ser
450 455 460
Ala Gly Thr Gly Met Pro Pro Pro Val Gly Met Asn Gly Ala Thr Pro
465 470 475 480
Arg Val Ala Ala Ala Pro Pro Thr Pro Pro Pro Val Ser Arg Pro Ala
485 490 495
Ala Pro Ala Ala Ala Pro Ala Ala Gly Ser Gly Phe Thr Met Pro Asn
500 505 510
Phe Ser Ala Ser Leu Ser Gly Leu Thr Gly Gly Ala Ala Ala Ala Ala
515 520 525
Lys Ser Ala Ala Asp Ala Ala Ser Ser Lys Leu Thr Lys Met Ala Asp
530 535 540
Ser Met Ser Ser Gly Ala Ala Ala Pro Ala Pro Pro Ala Ala Pro Ala
545 550 555 560
Arg Pro Ser Thr Ser Pro Arg Pro Ser Ala Ser Ser Pro Ile Ser Ser
565 570 575
Ser Ala Asp Ala Asp Arg Ser Asp Ser Ser Arg Arg Pro Val Asn Trp
580 585 590
Arg Asp Glu Leu Gln Ser Leu Lys Ala Thr Arg Glu Pro Asn Gly Asn
595 600 605
Gly Asn Gly Ser Gly Val Ala Pro Ala Ala Gly Arg Ala Asp Ala Asp
610 615 620
Glu Glu Ala Leu Arg Arg Phe Gly Asn Leu Ala Gly Arg Ser Arg Ser
625 630 635 640
Gly Asn Gly Gly Gly Gly Ser Ser Asp Thr Glu Leu Ser Glu Ala Asn
645 650 655
Arg Pro Arg Thr Arg Pro Asp Trp Arg Asn Gln Leu
660 665
<210> 25
<211> 381
<212> PRT
<213> 莱茵衣藻
<400> 25
Met Asn Asn Leu Arg Lys Lys Arg Phe Glu Glu Lys Thr Val Glu Arg
1 5 10 15
Val Tyr Glu Ala Asp Val Tyr Pro Ala Val Phe Asp Phe Glu Ala Trp
20 25 30
His Asn His Arg Ser Arg Lys Arg Tyr Leu Glu His Cys Ile Thr Leu
35 40 45
Phe Arg Ser Tyr Phe Phe Arg Asp Leu Leu Gly Pro Leu Ala Val Leu
50 55 60
Val Gly Thr Ala Val Ala Val Gly Cys Tyr Glu Gln Ala Leu Gln Asp
65 70 75 80
His Leu Leu Pro Gly Ala Leu Pro Ser Phe Ser Gly Val Ser Asp Thr
85 90 95
Pro Phe Gln Leu Thr Ser Phe Ala Leu Ser Leu Met Leu Val Phe Arg
100 105 110
Thr Asn Ser Ser Tyr Ala Arg Trp Leu Asp Ala Arg Gln Gln Trp Gly
115 120 125
Leu Ile Val Asn Thr Ser Arg Thr Phe Ile Arg Gln Val Met Thr Thr
130 135 140
Leu Pro Glu Ser Ser Cys Ser Glu Leu Arg Asp Ala Ile Ala Arg Trp
145 150 155 160
Thr Ile Ala Phe Val Arg Leu Ser Lys Leu His Leu Arg Glu His Gly
165 170 175
Asp Val Arg Leu Glu Met Ala Gly Val Leu Arg Gly Glu Glu Leu Pro
180 185 190
Leu Val Ala Ala Ala Ala His Arg Pro Leu Ala Ala Ala His Val Leu
195 200 205
Ser Glu Leu Leu Arg Ser Ala Glu Ala Ala Gly Arg Leu Ser Asp Gln
210 215 220
Ser Arg Ala Arg Leu Glu Ala Asp Val Asn Met Leu Ser Gln Ala Leu
225 230 235 240
Gly Ala Cys Glu Lys Ile Leu Arg Asn Pro Ile Pro Leu Ser Tyr Thr
245 250 255
Arg His Thr Ser Arg Phe Leu Val Leu Trp Leu Leu Trp Leu Pro Ile
260 265 270
Ala Leu Trp Gly Lys Val Ser Trp Gly Val Val Pro Val Glu Ala Ile
275 280 285
Leu Cys Tyr Leu Leu Leu Gly Ile Asp Glu Ile Ala Ile Gln Met Glu
290 295 300
Glu Pro Phe Gly Ile Leu Pro Leu Glu Asn Phe Cys Asp Ala Val Gln
305 310 315 320
Gln Ser Val Glu Gln Val Ala Ser Met Asp His Gly Val Gln Asp Val
325 330 335
Val Ala Ser Tyr Val Cys His Ile Val Gln Gln Asp Ser Gly Asn Gly
340 345 350
Thr Gly Ala Ser Glu Pro Ala Pro Pro Ala Glu Asp Gly Gly Asn Gly
355 360 365
Phe Ala Asn Ile Phe Phe Arg Met Pro Ala Asp Arg Cys
370 375 380
<210> 26
<211> 401
<212> PRT
<213> 莱茵衣藻
<400> 26
Met Gly Ser Gly Pro Arg Thr Ile Gly Leu Asp Ser Pro Thr Thr Thr
1 5 10 15
Ile Arg Ser Ala Ala Arg Asp Gln Arg Gln Leu Tyr Glu Val Glu Gly
20 25 30
Arg Gly Thr Val Pro Phe Asp His Tyr Lys Glu Gly Ser Arg His Ala
35 40 45
Arg Arg Met Thr Phe Thr Phe Asp Asp Trp Lys Arg His Arg Ser Ser
50 55 60
Asn Arg Tyr Leu Tyr His Leu Lys Thr Leu Thr Glu Ser Gly Ile Val
65 70 75 80
Arg Gly Ile Trp Ala Pro Val Ala Trp Val Thr Leu Phe Thr Ala Val
85 90 95
Val Ala Thr Leu Asn Val Ala His Gly Ala Ala Met Leu Pro Pro Trp
100 105 110
Val Pro Ala Met Pro Gln Ile Ala Ile Glu Pro Val Gln Leu Thr Ser
115 120 125
Ile Ala Leu Ser Leu Leu Leu Val Phe Arg Thr Asn Ala Ser Tyr Ser
130 135 140
Arg Trp Asp Glu Gly Arg Arg Ser Phe Gly Ser Ile Thr Thr Val Ser
145 150 155 160
Arg Asp Ile Ala Arg Gln Ala Phe Ala Trp Phe Arg Pro Asp Asp Tyr
165 170 175
Glu Ser Arg Val Arg Val Gly Arg Trp Leu Val Ala Leu Gly Arg Ser
180 185 190
Thr Met Val His Leu Arg Glu Glu His Asp Met Glu Asp Glu Leu Arg
195 200 205
Glu Val Leu Lys Pro Ala Glu Val Gln Ala Val Val Ser Ala Val His
210 215 220
Ala Pro Ser Phe Cys Leu Gln Met Ile Thr Leu Ile Ile Arg Thr Ala
225 230 235 240
Gly Leu Pro Gln Glu Leu Val Ile Arg Met Asp Glu Asn Val Ser Arg
245 250 255
Leu Thr Asp Ala Val Ser Ala Cys Glu Arg Ile Leu Asn Thr Pro Ile
260 265 270
Pro Leu Ser Tyr Thr Arg His Thr Ala Arg Phe Leu Met Ala Trp Leu
275 280 285
Ala Cys Leu Pro Phe Cys Leu Trp Thr Tyr Cys Gly Pro Ala Met Val
290 295 300
Pro Ile Ala Ala Leu Val Ala Phe Val Leu Leu Gly Ile Glu Glu Ile
305 310 315 320
Gly Val Tyr Ile Glu Glu Pro Phe Ser Ile Leu Ala Leu Glu Lys Leu
325 330 335
Val Asn Lys Leu Glu Asn Ile Val Asn Ala Met Leu Arg Asp Ser Gln
340 345 350
Glu Leu Asp Arg Leu Val Lys Val Gly Ser Pro Thr Ala Ser Pro Arg
355 360 365
Pro Glu Pro Ala Ser Thr Asn Ser Asn Asn Ala Val Thr Asp Thr Ala
370 375 380
Phe Gly Leu Gly Ala Ala Gly Gly Phe Ser Pro Ala Val Ala Gly Ala
385 390 395 400
Met
<210> 27
<211> 438
<212> PRT
<213> 莱茵衣藻
<400> 27
Met Gln Met Gln Ala Asn Arg Ser Ser Leu Arg Ala Ser Pro Val Arg
1 5 10 15
Gly Leu Gly Ala Arg Pro Leu Leu Arg Ala Leu Pro Ala Gly Arg Val
20 25 30
Ala Arg Leu Asn Val Ser Ala Gln Ala Lys Asp Pro Asn Ala Pro Ile
35 40 45
Gln Ser Asn Pro Leu Gly Thr Leu Ser Ser Gln Ser Gly Gln Val Ala
50 55 60
Thr Leu Pro Arg Ser Glu Glu Ala Arg Lys Tyr Phe Arg Thr Val Tyr
65 70 75 80
Asp Phe Pro Gln Trp Gln Lys His Arg Ser Ser Tyr Arg Phe Ala Glu
85 90 95
Arg Leu Phe Gln Leu Ser Gln Ser His Ile Leu Gln Asn Ala Leu Pro
100 105 110
Ala Ile Ser Trp Val Thr Leu Val Ala Thr Leu Val Ala Ser Tyr Gly
115 120 125
Tyr Ser Tyr Asp Gln His Met Leu Pro Asp Val Phe Pro Ser Ile Ser
130 135 140
Pro Asn Ala Ser Cys Thr Ala Phe Ile Ser Asn Thr Ser Val Ala Leu
145 150 155 160
Ser Leu Leu Leu Val Phe Arg Thr Asn Ser Ser Tyr Gly Arg Trp Asp
165 170 175
Glu Ala Arg Lys Met Trp Gly Gly Leu Leu Asn Arg Ser Arg Asp Ile
180 185 190
Met Arg Gln Gly Ala Thr Cys Phe Pro Asp Asp Gln Val Glu Ala Lys
195 200 205
Lys Ala Leu Ala Arg Trp Thr Val Ala Phe Ser Arg Ala Leu Arg Ile
210 215 220
His Phe Gln Pro Glu Val Thr Ile Glu Ser Glu Leu Gln Asn Ile Leu
225 230 235 240
Thr Pro Ala Glu Leu Gln Met Leu Ala Lys Ser Gln His Arg Pro Val
245 250 255
Arg Ala Ile His Ala Ile Ser Gln Ile Ile Gln Ser Val Pro Met Ser
260 265 270
Ser Ile His Gln Gln Gln Met Ser Asn Asn Leu Thr Phe Phe His Asp
275 280 285
Val Leu Gly Gly Cys Glu Arg Leu Leu Arg Ala Pro Ile Pro Val Ser
290 295 300
Tyr Thr Arg His Thr Ala Arg Phe Leu Phe Ala Trp Leu Thr Leu Leu
305 310 315 320
Pro Phe Ala Leu Tyr Pro Thr Thr Gly Trp Gly Val Val Pro Val Cys
325 330 335
Thr Gly Ile Ala Ala Val Leu Cys Gly Ile Glu Glu Ile Gly Val Gln
340 345 350
Cys Glu Glu Pro Phe Gly Ile Leu Pro Leu Asp Val Ile Cys Asn Arg
355 360 365
Ile Gln Ala Asp Val Met Ala Thr Leu Lys Asp Asp Ala Asp Thr Lys
370 375 380
Thr Ile Leu Ala Glu Ala Gly Leu Ile Ser Leu Ile Pro Ser Ala Thr
385 390 395 400
Ser Ala Thr Pro Val Ala Ser Ala Glu Pro Val Leu Val Ser Ala Arg
405 410 415
Pro Ser Ala Ala Pro Ala Pro Asn Asn Gly Leu Gln Val Arg Val Ala
420 425 430
Met Gly Gly Glu Arg Lys
435
<210> 28
<211> 450
<212> PRT
<213> 莱茵衣藻
<400> 28
Met Gln Cys Leu Ser Ser Arg Pro Val Ala Met Gly Arg Ala Gly Ser
1 5 10 15
Ser Ala Leu Pro Arg Leu Pro Leu Arg Ala Gly Arg Val Cys His Leu
20 25 30
Gly Val Arg Cys Gln Ala Ala Asn Lys Asp Pro Asn Ala Pro Ile Gln
35 40 45
Ser Asn Pro Leu Gly Ser Phe Ser Ser Gln Leu Gln Asn Gln Pro Thr
50 55 60
Leu Pro Arg Ser Glu Glu Ala Arg Lys Tyr Phe Arg Thr Val Tyr Asp
65 70 75 80
Phe Pro Gln Trp Gln Thr His Arg Asn Gln Tyr Arg Leu Met Lys Arg
85 90 95
Leu Phe Ser Ile Pro Gln Ser His Val Ile Gln Asn Ala Leu Pro Ser
100 105 110
Ile Met Trp Val Ala Phe Thr Ser Thr Cys Val Ala Ala Tyr Met Tyr
115 120 125
Gly Tyr Asp Gln His Met Leu Pro Glu Gly Phe Pro Thr Leu Ala Pro
130 135 140
Asn Ala Ala Cys Ser Ala Phe Ile Ser Asn Thr Ser Val Ala Leu Ser
145 150 155 160
Leu Leu Leu Val Phe Arg Thr Asn Ser Ser Tyr Gly Arg Trp Asp Glu
165 170 175
Ala Arg Lys Met Trp Gly Gly Leu Leu Asn Arg Ser Arg Asp Ile Met
180 185 190
Arg Gln Gly Ala Thr Cys Phe Pro Asp Asp Gln Val Glu Ala Lys Lys
195 200 205
Ala Leu Ala Arg Trp Val Val Ala Phe Ser Arg Ala Leu Arg Ile His
210 215 220
Phe Gln Pro Glu Val Thr Ile Glu Ser Glu Leu Lys Asn Ile Leu Thr
225 230 235 240
Pro Ala Glu Leu Gln Met Leu Ala Lys Ser Gln His Arg Pro Val Arg
245 250 255
Ala Ile His Ala Ile Ser Gln Ile Ile Gln Ser Val Pro Met Ser Ser
260 265 270
Ile His Gln Gln Gln Met Ser Asn Asn Leu Thr Phe Phe His Asp Val
275 280 285
Leu Gly Gly Cys Glu Arg Leu Leu Arg Ala Pro Ile Pro Val Ser Tyr
290 295 300
Thr Arg His Thr Ala Arg Phe Leu Phe Ala Trp Leu Thr Leu Leu Pro
305 310 315 320
Phe Ala Leu Tyr Gly Ser Cys Gly Val Ser Val Ile Pro Val Cys Ser
325 330 335
Gly Ile Ala Ala Val Leu Cys Gly Ile Glu Glu Ile Gly Val Gln Cys
340 345 350
Glu Glu Pro Phe Gly Ile Leu Pro Leu Asp Val Ile Cys Asn Arg Ile
355 360 365
Gln Ala Asp Val Met Ala Thr Leu Lys Asp Asp Ala Asp Thr Lys Thr
370 375 380
Ile Leu Ala Glu Ala Gly Leu Ile Ser Leu Arg Ala Asn Ser Ala Met
385 390 395 400
Ala Val Glu Asn Ala Leu Pro Asp Leu Asp Ser Ile Asn Ala Ala Ala
405 410 415
Pro Asn Gly Asn Gly Ser His Asn Gly Asn Gly Ala Ala Val Pro Val
420 425 430
Ser Val Ser Ala Gly Ala Ser Gly Asn Gly Met Asn Val Arg Ile Ser
435 440 445
Pro Arg
450
<210> 29
<211> 466
<212> PRT
<213> 莱茵衣藻
<400> 29
Met Gln Val Ser Lys Val Pro Ser Ser Ala Ser Ala Arg Cys Leu Pro
1 5 10 15
Arg Leu Pro Val Arg Thr Ser Arg Val Cys Gln Leu Ser Val Arg Cys
20 25 30
Gln Ala Ala Asn Lys Asp Pro Asn Ala Pro Ile Gln Ser Asn Pro Leu
35 40 45
Gly Ser Phe Ser Ser Gln Asn Ser Ser Gly Ala Val Val Thr Ala Pro
50 55 60
Arg Asn Glu Asp Ala Arg Lys Tyr Phe Arg Thr Val Tyr Asp Phe Pro
65 70 75 80
Gln Trp Gln Lys His Arg Ser Gln Ser Arg Leu Val Arg Arg Leu Phe
85 90 95
Thr Ile Pro Gln Ser His Val Ile Gln Asn Ala Leu Pro Ser Ile Met
100 105 110
Trp Val Thr Phe Thr Ser Thr Cys Val Ala Ala Tyr Met Tyr Gly Tyr
115 120 125
Asp Leu His Ile Leu Pro Glu Gly Phe Pro Thr Leu Ala Pro Asn Ala
130 135 140
Ala Cys Ser Ala Phe Ile Ser Asn Thr Ser Val Ala Leu Ser Leu Leu
145 150 155 160
Leu Val Phe Arg Thr Asn Ser Ser Tyr Gly Arg Trp Asp Glu Ala Arg
165 170 175
Lys Met Trp Gly Gly Leu Leu Asn Arg Ser Arg Asp Ile Met Arg Gln
180 185 190
Gly Ala Thr Cys Phe Pro Asp Asp Gln Val Glu Ala Lys Lys Ala Leu
195 200 205
Ala Arg Trp Thr Val Ala Phe Ala Arg Ala Leu Arg Ile His Phe Gln
210 215 220
Pro Glu Val Thr Ile Glu Ser Glu Leu Gln Asn Ile Leu Thr Pro Ala
225 230 235 240
Glu Leu Gln Met Leu Ala Lys Ser Gln His Arg Pro Val Arg Ala Ile
245 250 255
His Ala Ile Ser Gln Ile Ile Gln Ser Val Arg Met Ser Ser Ile His
260 265 270
Gln Gln Gln Met Ser Asn Asn Leu Thr Phe Phe His Asp Val Leu Gly
275 280 285
Gly Cys Glu Arg Leu Leu Arg Ala Pro Ile Pro Val Ser Tyr Thr Arg
290 295 300
His Thr Ala Arg Phe Leu Phe Ala Trp Leu Thr Leu Leu Pro Phe Ala
305 310 315 320
Leu Tyr Gly Ser Cys Gly Val Ser Val Ile Pro Val Cys Thr Gly Ile
325 330 335
Ala Ala Val Leu Cys Gly Ile Glu Glu Ile Gly Val Gln Cys Glu Glu
340 345 350
Pro Phe Gly Ile Leu Pro Leu Asp Val Ile Cys Asn Arg Ile Gln Ala
355 360 365
Asp Val Met Ala Thr Leu Lys Asp Asp Ala Asp Thr Lys Thr Val Leu
370 375 380
Ala Glu Ala Gly Leu Ile Ser Leu Ile Pro Ser Met Ser Leu Pro Pro
385 390 395 400
Thr Glu His Ala Ser Pro Ser Asp Pro Val Thr Ala Ala Ala Ala Ala
405 410 415
Ala Leu Ala Ala Ala Asn Gly Asn Gly Ala Ala Ser His Ser Asn Gly
420 425 430
Asn Gly Ser Lys Pro Val Ser Thr Gln Val Pro Pro Pro Val Leu Ala
435 440 445
Pro Val Thr Val Thr Ser Ser Ser Gly Ser Met Asn Val Arg Ile Ser
450 455 460
Pro Arg
465
<210> 30
<211> 456
<212> PRT
<213> 金色藻属 CCMP291
<400> 30
Met Arg Glu His Pro Leu Ser Tyr Glu Glu Tyr Met Arg Gln Arg Ser
1 5 10 15
Ala Gly Arg Asp Pro Leu Ala Glu Ala Val Gln Gly Gln Ser Ala Ser
20 25 30
Met Glu Arg Gly Val Val Pro Pro Pro Pro Val Lys Ala Thr Pro Glu
35 40 45
Arg Thr Glu Ala Ala Phe Thr Pro Pro Ala Glu Phe Asp Phe Phe Gln
50 55 60
Asp Val Phe Lys Pro Thr Val Glu Ser Val Val Lys Ala Val Val Ser
65 70 75 80
Pro Gly Ala Gln Gln Asp Ser Asp Glu Ser Tyr Met Arg Val Pro Trp
85 90 95
Trp Glu Gln Gly Ser Thr Tyr Ser Glu Asp Gln Arg Lys Asp Arg Arg
100 105 110
Thr Val Phe Met His Asp Asp Trp Lys Arg His Arg Ser Ser Glu Arg
115 120 125
Phe Phe Arg Asn Ile Lys Thr Trp Pro Ser Ser Gly Ile Asn Gln Ala
130 135 140
Leu Arg Lys Glu Leu Thr Phe Val Thr Ser Val Ser Val Phe Val Val
145 150 155 160
Leu Ala Asn Met Leu Leu Tyr Gln Tyr Gln Asp Phe Gly Gly Val Val
165 170 175
His Pro Gly Pro Leu Ser Phe Leu Asp Gly Pro Ile Lys Ser Leu Ser
180 185 190
Leu Pro Ala Leu Pro Phe Ser Met Ala Ser Pro Val Leu Ser Leu Leu
195 200 205
Leu Val Phe Arg Thr Asn Thr Ala Tyr Phe Arg Trp Asn Glu Ala Arg
210 215 220
Thr Leu Trp Gly Gly Leu Ile Asn Asn Cys Arg Asn Ile Val Arg Gln
225 230 235 240
Thr Thr Thr Met Phe Pro Asn Asp Ala Tyr His Asn Ala Leu Lys Lys
245 250 255
Arg Leu Ala Thr Glu Thr Ala Thr Phe Ile Lys Ser Leu Arg Asn Phe
260 265 270
Leu Arg Gly Pro Glu Asp Asp Ala Thr Leu Arg Lys Glu Leu Tyr Ala
275 280 285
Tyr Val Asn Gln Gly Leu Met Thr Ser Ala Gln Ala Glu Ala Thr Leu
290 295 300
Ala Ala Lys Asn Arg Pro Met Phe Ala Leu Ala Ala Met Ser Ala Thr
305 310 315 320
Leu Arg Lys Ala Asn Ile Asp Glu Met Tyr Ile Ser Arg Met Asp Ser
325 330 335
Thr Ile Ser Val Leu Val Asp Leu Thr Gly Ala Asn Glu Arg Ile Phe
340 345 350
Lys Ser Pro Ile Pro Leu Val Tyr Thr Arg Leu Thr Ala Arg Phe Leu
355 360 365
Ser Val Phe Leu Thr Leu Leu Pro Leu Ala Met Trp Ala Ala Leu Gly
370 375 380
Glu Ser Trp Asn His Trp Ala Thr Ile Pro Ala Thr Phe Ile Leu Ser
385 390 395 400
Val Phe Leu Phe Gly Val Glu Glu Val Gly Ile Gln Ile Glu Glu Pro
405 410 415
Phe Ser Ile Leu Pro Leu Glu Ala Met Cys Asn Gly Ala Ile Glu Ala
420 425 430
Val Gln Leu Glu Met Leu Ala Ala Glu Gln Ser Gln Val Phe Glu Ala
435 440 445
Ala Gly Asp Ala Val Ala Val Ala
450 455
<210> 31
<211> 415
<212> PRT
<213> Fistulifera solaris
<400> 31
Met Arg Phe Thr Thr Arg Gln Leu Ile Val Gly Ile Ala Leu Thr Ala
1 5 10 15
Ser Leu His Gly Ala Phe Ala Phe Val Thr Pro Ser Val Val Gly Ser
20 25 30
Ser Lys Ala Ser Lys Ser Ala Leu Asp Val Ser Thr Val Pro Arg Asn
35 40 45
Asp Gly Ser Gly Leu Ser Tyr Gly Glu Arg Ser Arg Pro Phe Arg Arg
50 55 60
Asn Phe Tyr Thr Gln Pro Asp Trp Glu Lys His Arg Ser Arg Asn Arg
65 70 75 80
Phe Lys Gly Asn Leu Leu Ser Ile Thr Arg Ser Gly Val Ile Arg Gln
85 90 95
Leu Thr Asn Glu Val Leu Phe Ile Thr Ala Val Ala Thr Ala Val Trp
100 105 110
Ala Ile Asn Cys Leu Thr Ala Ala Gly Tyr Asp Asp Leu Asn Gly Val
115 120 125
His His Glu Gly Leu Leu Pro Phe Phe Pro Thr Leu Gln Met Pro Ser
130 135 140
Glu Phe Phe Thr Phe Ser Ser Pro Ala Leu Ser Leu Leu Leu Val Phe
145 150 155 160
Lys Thr Asn Thr Ser Tyr Gly Arg Trp Asp Glu Ala Arg Lys Ala Trp
165 170 175
Gly Val Ile Val Asn Ser Ser Arg Thr Ile Leu Arg Gln Gly Ala Ala
180 185 190
Trp Leu Gln Glu Thr Asn Ile Pro Glu Arg Asp Lys Ile Arg Leu Ile
195 200 205
Ala Arg Leu Ala Ala Ala Val Trp Cys Phe Pro Arg Ser Met Thr Arg
210 215 220
His Val Leu Ser Ala Arg Glu Asp Asp Ala Ala Tyr Ala Glu Asp Cys
225 230 235 240
Arg Ala Asn Leu Arg Pro Asp Leu Ala Glu Asp Leu Ile Ala Ala Arg
245 250 255
His Lys Pro Thr Arg Ala Thr Tyr Glu Ile Ser Cys Ala Ile Asn Asp
260 265 270
Leu Pro Leu Ser Asp Ile Gln Lys Ala Thr Leu Glu Tyr Ser Val Asn
275 280 285
Gln Leu Cys Asp Ala Met Gly Ala Asn Asp Arg Ile Leu Ser Ser Pro
290 295 300
Val Pro Leu Val Tyr Thr Arg His Leu Ala Arg Phe Leu Glu Val Trp
305 310 315 320
Leu Leu Met Leu Pro Leu Gly Leu Trp Pro Asp Phe Thr Asn Ser Trp
325 330 335
Asn His Ile Ala Met Ile Pro Ile Thr Val Leu Leu Ser Tyr Phe Leu
340 345 350
Leu Gly Ile Glu Glu Leu Gly Val Gln Leu Glu Glu Pro Phe Ser Val
355 360 365
Leu Pro Leu His Lys Ile Thr Ser Gly Ile Gly Leu Ser Ala Glu Glu
370 375 380
His Val Gln Trp Phe Leu Asn Asp Ser Ala Lys Ser Glu Met Lys Thr
385 390 395 400
Asn Ser Ala Gly Ser Thr Ser Pro Asn Tyr Gln Ser Leu Ser Pro
405 410 415
<210> 32
<211> 430
<212> PRT
<213> Fistulifera solaris
<400> 32
Met Met Leu Arg Leu Ser Asn Ala Val Phe Ala Leu Ile Ala Leu Ala
1 5 10 15
Ser Val Ser Gln Gly Ser Ala Phe Ala Pro Ser Ser Thr Ser Ser Arg
20 25 30
Val Asp Thr Ala Leu Phe Gly Asn Thr Gln Ser Pro Pro Pro Ile Pro
35 40 45
Met Pro Lys Ala Ile Ser Tyr Gly Glu Glu Ser Arg Lys Tyr Arg Arg
50 55 60
Thr Val Phe Thr His Asp Asp Trp Val Lys His Arg Ser Pro Glu Arg
65 70 75 80
Phe Val Arg Asn Leu Ser Ser Ile Trp Ser Ser Gly Val Tyr Lys Asn
85 90 95
Leu Ala Asn Glu Val Met Ala Thr Thr Gly Val Ala Thr Phe Val Val
100 105 110
Leu Tyr Asn Ala Leu Val Gly Gly Tyr Thr Asp Phe Ser Gly Val Ala
115 120 125
His Ala Ala Pro Ile Gln Gly Leu Met Gln Val Gly Leu Pro Leu Ala
130 135 140
Pro Phe Thr Leu Ser Ser Pro Ser Leu Gly Leu Leu Leu Val Phe Arg
145 150 155 160
Thr Asn Thr Ser Tyr Gln Arg Trp Asp Glu Ala Arg Lys Asn Trp Gly
165 170 175
Met Asn Ile Asn His Thr Arg Asp Leu Val Arg Met Gly Thr Ala Met
180 185 190
Tyr Asp Arg Gln Gly Val Ser Pro Glu Lys Val Glu Lys Asp Leu Lys
195 200 205
Thr Leu Ser Leu Cys Thr Trp Ala Phe Val Arg Ala Met Lys Arg His
210 215 220
Leu Ser Pro Glu Ser Glu Asp Glu Glu Ala Phe Lys Arg Glu Leu Tyr
225 230 235 240
Glu Lys Leu Pro Ser Ala Gln Ala Glu Ala Ile Ile Lys Ala Ala His
245 250 255
Arg Pro Asn Arg Ala Leu Phe Asp Leu Ser Leu Ala Ile Glu Asn Leu
260 265 270
Asn Met His Phe Met Arg Lys Asn Glu Ile His Lys Ala Ala Thr Ile
275 280 285
Phe Glu Asp Asn Leu Gly Ser Ser Glu Arg Leu Leu Thr Ser Pro Val
290 295 300
Pro Leu Phe Tyr Ser Arg His Leu Ala Arg Phe Leu Ser Leu Trp Leu
305 310 315 320
Leu Leu Ile Pro Phe Ala Leu Tyr Asp Thr Leu Gly Gly Thr Trp Asn
325 330 335
His Val Gly Leu Ile Pro Thr Thr Ala Val Leu Ser Thr Phe Leu Phe
340 345 350
Gly Ile Glu Glu Leu Ala Ala Gln Met Glu Glu Pro Phe Thr Ile Leu
355 360 365
Pro Met Gln Ala Phe Cys Asp Lys Ile Gly Asn Trp Cys Asn Glu Ile
370 375 380
Val Ser Trp Ser Pro Gly Asp Asn Gly Met Glu Val Thr Ile Pro Ser
385 390 395 400
Ala Val Glu Gln Asp His Val Val Glu Glu Met Met Val Ala Asn Gly
405 410 415
Ser Thr Arg Pro Ser Leu Arg Lys Val Leu Gly Leu Lys Lys
420 425 430
<210> 33
<211> 430
<212> PRT
<213> Fistulifera solaris
<400> 33
Met Met Leu Arg Leu Ser Ser Ala Val Phe Ala Leu Ile Ala Leu Ala
1 5 10 15
Ser Val Ser Gln Gly Ser Ala Phe Thr Pro Ser Ser Thr Ser Ser Arg
20 25 30
Val Asp Thr Ala Leu Phe Gly Asn Thr Gln Ser Pro Pro Pro Ile Pro
35 40 45
Met Pro Lys Ala Ile Ser Tyr Gly Glu Glu Ser Arg Lys Tyr Arg Arg
50 55 60
Thr Val Phe Thr His Asp Asp Trp Val Lys His Arg Ser Pro Glu Arg
65 70 75 80
Phe Val Arg Asn Leu Ser Ser Ile Trp Ser Ser Gly Val Tyr Lys Asn
85 90 95
Leu Ala Asn Glu Val Met Ala Thr Thr Gly Val Ala Thr Phe Val Val
100 105 110
Ile Tyr Asn Ala Leu Val Gly Gly Tyr Thr Asp Phe Ser Gly Val Ala
115 120 125
His Ala Ala Pro Ile Gln Gly Leu Leu Gln Val Gly Leu Pro Leu Ala
130 135 140
Pro Phe Thr Leu Ser Ser Pro Ser Leu Gly Leu Leu Leu Val Phe Arg
145 150 155 160
Thr Asn Thr Ser Tyr Gln Arg Trp Asp Glu Ala Arg Lys Asn Trp Gly
165 170 175
Met Asn Ile Asn His Thr Arg Asp Leu Val Arg Met Gly Thr Ala Met
180 185 190
Tyr Asp Arg Gln Gly Val Pro Pro Glu Lys Val Glu Lys Asp Leu Lys
195 200 205
Met Leu Ser Leu Cys Thr Trp Ala Phe Val Arg Ala Met Lys Arg His
210 215 220
Leu Ser Pro Glu Ser Glu Asp Glu Glu Ala Phe Lys Arg Glu Leu Tyr
225 230 235 240
Glu Lys Leu Pro Ser Ala Gln Ala Glu Ala Ile Ile Lys Ala Ala His
245 250 255
Arg Pro Asn Arg Ala Leu Phe Asp Leu Ser Leu Ala Ile Glu Asn Leu
260 265 270
Asn Met His Phe Met Arg Lys Asn Glu Ile His Lys Ala Ala Thr Ile
275 280 285
Phe Glu Asp Asn Leu Gly Ser Ser Glu Arg Leu Leu Thr Ser Pro Val
290 295 300
Pro Leu Phe Tyr Ser Arg His Leu Ala Arg Phe Leu Ser Leu Trp Leu
305 310 315 320
Leu Leu Ile Pro Phe Ala Leu Tyr Asp Pro Leu Ala Gly Thr Trp Asn
325 330 335
His Val Gly Leu Ile Pro Thr Thr Ala Met Leu Ser Val Phe Leu Phe
340 345 350
Gly Ile Glu Glu Leu Ala Ala Gln Met Glu Glu Pro Phe Thr Ile Leu
355 360 365
Pro Met Gln Ala Phe Cys Asp Lys Ile Gly Asn Trp Cys Asn Glu Ile
370 375 380
Val Ser Trp Ser Pro Gly Asp Asn Gly Met Glu Val Thr Ile Pro Ser
385 390 395 400
Ala Val Glu Glu Asp His Val Val Glu Glu Met Met Val Ala Asn Gly
405 410 415
Ser Thr Arg Pro Ser Leu Arg Lys Val Leu Gly Leu Lys Lys
420 425 430
<210> 34
<211> 505
<212> PRT
<213> Fistulifera solaris
<400> 34
Met Val Arg Phe Thr Cys Ile Phe Pro Leu Val Ala Ala Leu Ile Ser
1 5 10 15
Asn Ala Ser Ala Phe Val Thr Pro Ala Asn Ser Val Ala Lys His Ala
20 25 30
Thr Pro Arg Phe Glu Gly Asn Glu Arg Arg Leu Ile Ser Ala Pro Ser
35 40 45
Asp Gly Ser Val Ala Lys Arg Ser Thr Ser Arg Phe Ser Ser Glu Gly
50 55 60
Thr Gly Trp Asp Ser Phe Lys Gly Leu Arg Asp Phe Thr Asn Ile Pro
65 70 75 80
Ser Gly Glu Glu Gln Arg Lys Phe Arg Arg Thr Val Tyr Thr His Lys
85 90 95
Asp Trp Lys Lys His Arg Ser Gln Asp Arg Phe Val Phe Tyr Leu Leu
100 105 110
Ala Met Phe Lys Ser Gly Val Tyr Lys Asn Ile Gly Arg Glu Val Ile
115 120 125
Thr Ile Thr Ala Val Ala Ala Phe Val Cys Ile Tyr Asn Ser Leu Val
130 135 140
Gly Gly Phe Thr Asp Leu Asn Gly Val Gln Gln Ala Ala Leu Leu Gln
145 150 155 160
Ser Gln Phe Leu Pro Lys Leu Thr Leu Pro Leu Ser Leu Phe Thr Ile
165 170 175
Thr Ser Ser Ser Leu Gly Leu Leu Leu Val Phe Arg Thr Asn Thr Ser
180 185 190
Tyr Gln Arg Trp Asp Glu Ala Arg Lys Asn Trp Gly Met Asn Ile Asn
195 200 205
His Thr Arg Asp Leu Val Arg Met Ala Asn Ala Tyr Tyr Asp Ser Thr
210 215 220
Gly Val Pro Pro Glu Val Arg Ala Asp Asp Met Asn His Val Ala Leu
225 230 235 240
Cys Thr Trp Ala Phe Val Arg Cys Met Lys Arg His Leu Ser Pro Glu
245 250 255
Glu Glu Asp Glu Ala Asp Phe Gln Lys Glu Ile Leu Glu Lys Leu Pro
260 265 270
Arg Gln Gln Ala Glu Met Ile Ile Ala Ala Ala His Arg Pro Asn Arg
275 280 285
Ala Leu Gln Asp Leu Ser Tyr Ala Ile Asp Asp Leu Pro Met His Phe
290 295 300
Ile Arg Lys Asn Glu Ile His Arg Ala Val Thr Ile Phe Glu Asp Asn
305 310 315 320
Leu Gly Ser Ser Glu Arg Leu Leu Ser Ser Pro Val Pro Val Phe Tyr
325 330 335
Ser Arg His Leu Ala Arg Phe Leu Ala Val Trp Leu Leu Phe Val Pro
340 345 350
Phe Ala Leu Tyr Asp Ser Phe Asn Ala Ser Trp Asn His Ile Ala Met
355 360 365
Val Pro Ala Thr Ala Val Met Ser Ile Phe Leu Val Gly Ile Glu Glu
370 375 380
Leu Gly Thr Gln Leu Glu Glu Pro Phe Thr Ile Leu Pro Met Gln Gly
385 390 395 400
Phe Cys Asp Lys Ile Tyr Asn Trp Cys Met Glu Ile Ala Ser Trp Gln
405 410 415
Pro Gly Asp Asn Gly Arg Pro Met Arg Pro Val Lys Pro Glu His Ala
420 425 430
Tyr Phe Thr Ser Thr Ser Glu Gly Val Asn Gly Tyr Ala Asn Asn Tyr
435 440 445
Tyr Thr Pro Gln Gln Ser Tyr Tyr Asn Glu Pro Ala Gln Pro Gln Gln
450 455 460
Ser Tyr Tyr Ser Glu Pro Thr Pro Ala Pro Gln Tyr Tyr Asn Glu Pro
465 470 475 480
Thr Pro Ala Gln Pro Ala Ala Asn Gln Gly Trp Val Asp Met Ala Glu
485 490 495
Phe Leu Arg Arg Gln Gly Ser Gly Val
500 505
<210> 35
<211> 502
<212> PRT
<213> Fistulifera solaris
<400> 35
Met Val Arg Met Ile Cys Ile Leu Pro Leu Val Ala Ala Leu Ile Gly
1 5 10 15
Ser Ala Asn Ala Leu Val Ala Pro Ala Asn Asn Val Ala Lys Gln Ala
20 25 30
Met Pro Arg Phe Ser Asn Asn Val Ala Lys Ser Ala Val Pro Arg Leu
35 40 45
Leu Thr Ser Glu Gly Thr Arg Leu Phe Ser Ser Glu Gly Thr Gly Trp
50 55 60
Asp Ser Phe Lys Gly Val Arg Asp Phe Thr Asn Ile Pro Ser Gly Glu
65 70 75 80
Glu Gln Arg Lys Phe Arg Arg Thr Val Tyr Thr His Lys Asp Trp Lys
85 90 95
Lys His Arg Ser Gln Asp Arg Phe Ile Tyr Tyr Leu Leu Ala Ile Phe
100 105 110
Lys Ser Gly Val Tyr Lys Asn Ile Gly Arg Glu Val Ile Thr Ile Thr
115 120 125
Ala Val Ala Ala Leu Val Cys Val Tyr Asn Ser Leu Val Gly Gly Tyr
130 135 140
Thr Asp Leu Asn Gly Val Gln Gln Ala Ala Leu Leu Gln Ser Gln Phe
145 150 155 160
Leu Pro Lys Leu Thr Leu Pro Leu Ser Leu Phe Thr Ile Thr Ser Ser
165 170 175
Ser Leu Gly Leu Leu Leu Val Phe Arg Thr Asn Thr Ser Tyr Gln Arg
180 185 190
Trp Asp Glu Ala Arg Lys Asn Trp Gly Met Asn Ile Asn His Thr Arg
195 200 205
Asp Leu Val Arg Met Ala Asn Ala Tyr Tyr Asp Ser Thr Gly Val Pro
210 215 220
Pro Glu Val Arg Ala Asp Asp Met Asn His Ile Ala Leu Cys Thr Trp
225 230 235 240
Ala Phe Val Arg Cys Met Lys Arg His Leu Ser Pro Glu Glu Glu Asp
245 250 255
Glu Leu Asp Phe Gln Lys Glu Ile Leu Glu Lys Leu Pro Arg Lys Gln
260 265 270
Ala Glu Met Ile Ile Ala Ala Ala His Arg Pro Asn Arg Ala Leu Gln
275 280 285
Asp Leu Ser Tyr Ala Ile Asp Asp Leu Pro Met His Phe Met Arg Lys
290 295 300
Asn Glu Ile His Arg Ala Val Thr Ile Phe Glu Asp Asn Leu Gly Ser
305 310 315 320
Ser Glu Arg Leu Leu Ser Ser Pro Val Pro Val Phe Tyr Ser Arg His
325 330 335
Leu Ala Arg Phe Leu Ala Val Trp Leu Leu Phe Val Pro Phe Ala Leu
340 345 350
Tyr Asp Ser Phe Asn Ala Ser Trp Asn His Ile Ala Met Val Pro Ala
355 360 365
Thr Ala Val Met Ser Ile Phe Leu Val Gly Ile Glu Glu Leu Gly Thr
370 375 380
Gln Leu Glu Glu Pro Phe Thr Ile Leu Pro Met Gln Gly Phe Cys Asp
385 390 395 400
Lys Ile Tyr Asn Trp Cys Met Glu Ile Ala Ser Trp Gln Pro Gly Asp
405 410 415
Asn Gly Arg Ser Met Arg Pro Val Lys Pro Glu His Ala Tyr Phe Thr
420 425 430
Ser Thr Ser Glu Gly Val Asn Gly Tyr Ser Asn Asn Tyr Tyr Thr Pro
435 440 445
Gln Gln Ser Tyr Tyr Asn Glu Pro Ala Gln Pro Gln Gln Ser Tyr Tyr
450 455 460
Ser Glu Pro Thr Pro Ala Pro Gln Tyr Tyr Asn Glu Pro Thr Pro Ala
465 470 475 480
Gln Pro Ala Gly Asn Gln Gly Trp Val Asp Met Ala Glu Phe Leu Arg
485 490 495
Arg Gln Gly Ser Gly Val
500
<210> 36
<211> 433
<212> PRT
<213> 圆柱拟脆杆藻 CCMP1102
<400> 36
Met Gly Leu Ala Ser Thr Leu Ile Glu Glu Val Val Glu Glu Glu Val
1 5 10 15
Trp Val Pro Pro Ser Ser Phe Ser Thr Pro Asp Gln Tyr Asn Ser Ser
20 25 30
Pro Thr Leu Ser Ala Gln Ser Pro Thr Ile Ser Val Thr Leu Pro Val
35 40 45
Gly Leu Arg Pro Pro Lys Phe Gly Pro Lys Lys Asp Ile Thr Tyr Gly
50 55 60
Glu Asn Ser Arg Lys Phe Arg Arg Thr Val Tyr Thr His Glu Asp Trp
65 70 75 80
Val Arg His Arg Ser Pro Asp Arg Phe Phe Arg Tyr Ile Ala Ser Val
85 90 95
Pro Thr Ser Gly Ile Ala Lys Asn Leu Ala Arg Glu Leu Tyr Phe Leu
100 105 110
Thr Ala Ile Thr Ala Ile Val Val Leu Tyr Asn Gly Leu Ile Thr Gly
115 120 125
Tyr Gln Asp Ile Asn Gly Ile Ala His Gly Pro Val Trp Ser Ser Pro
130 135 140
Phe Leu Pro Pro Leu Thr Leu Pro Met Gln Pro Phe Ala Leu Ala Ser
145 150 155 160
Pro Ser Leu Gly Leu Leu Leu Val Phe Arg Ser Asn Thr Ala Tyr Gln
165 170 175
Arg Trp Asp Glu Ala Arg Lys Asn Trp Gly Met Asn Ile Asn His Thr
180 185 190
Arg Asp Leu Asn Arg Met Ala Asn Ala Tyr Tyr Asp Lys Thr Gly Ile
195 200 205
Ser Asp Glu Lys Arg Lys Asp Asp Leu Glu Arg Leu Ser Leu Cys Thr
210 215 220
Trp Ala Phe Val Arg Ala Met Lys Arg His Leu Ser Pro Ala Asp Glu
225 230 235 240
Asp Glu Ala Ala Phe Lys Ala Glu Leu Tyr Glu Lys Leu Pro Lys Leu
245 250 255
Gln Ala Gln Gln Ile Ile Lys Ala Ala His Arg Pro Asn Arg Ala Leu
260 265 270
Tyr Asp Leu Ser Leu Ala Ile Glu Asn Leu Pro Met His Phe Leu Arg
275 280 285
Lys Asn Lys Leu His Ala Ala Leu Thr Ile Phe Glu Asp Thr Leu Gly
290 295 300
Ser Ser Glu Arg Leu Leu Thr Ser Pro Val Pro Leu Ile Tyr Asn Arg
305 310 315 320
His Thr Ser Arg Phe Leu Leu Val Trp Leu Leu Leu Leu Pro Phe Ala
325 330 335
Leu Trp Glu Glu Phe Gly Phe Met Trp Asn His Ile Gly Met Ile Pro
340 345 350
Gly Met Ser Ile Leu Gly Leu Leu Leu Phe Gly Ile Glu Glu Leu Ala
355 360 365
Thr Gln Leu Glu Glu Pro Phe Thr Ile Leu Pro Met Gln Ala Phe Cys
370 375 380
Asp Lys Ile Trp Asn Trp Cys Asn Glu Ile Ala Ser Trp Glu Asp Gly
385 390 395 400
Gln Asn Gly Ile Thr Thr Asn Arg Ser Tyr Gln Gly Arg Thr Glu Val
405 410 415
Tyr Val Asp Asn Ser Tyr Glu Asn Glu Asn Ala Tyr Thr Thr Pro Thr
420 425 430
Pro
<210> 37
<211> 430
<212> PRT
<213> 圆柱拟脆杆藻 CCMP1102
<400> 37
Met Arg Phe Val Gly Val Ser Ser Leu Met Ser Met Ala Ala Val Met
1 5 10 15
Thr Val Val Val Val Ser Cys His Val Gly Ser Ala Ser Ala Phe Val
20 25 30
Pro Pro Ser Gly Lys Thr Ser Ser Ser Trp Asn Ser Val Ala Ser Gln
35 40 45
Gln Ala Lys Asp Pro Leu His Ile Leu Pro Pro Asp Ala Thr Asp Ile
50 55 60
Pro Tyr Gly Glu Glu Ser Arg Lys Phe Arg Arg Thr Val Tyr Thr His
65 70 75 80
Asp Asp Trp Ile Lys His Arg Ser Pro Asp Arg Phe Phe Lys Thr Leu
85 90 95
Arg Thr Thr Thr Thr Ser Gly Ile Tyr Lys Asn Val Gly Arg Glu Val
100 105 110
Leu Ala Val Thr Phe Ile Ala Thr Phe Val Met Leu Trp Asn Met Leu
115 120 125
Thr Gly Glu Tyr Thr Asp Leu Val Gly Leu Val His Pro Gly Ile Met
130 135 140
Arg Gly Gln Phe Thr Pro Ser Leu Ala Leu Pro Met Ser Pro Phe Thr
145 150 155 160
Leu Ser Ser Gly Phe Leu Gly Leu Leu Leu Val Phe Arg Thr Asn Lys
165 170 175
Ala Tyr Gln Arg Trp Asp Glu Ala Arg Lys Asn Trp Gly Met Asn Ile
180 185 190
Asn His Thr Arg Asp Leu Val Arg Met Gly Thr Thr Phe Tyr Asp Asp
195 200 205
Ala Gln Ile Ser Asn Pro Glu Gln Arg Asp Ile Asp Leu Glu Arg Leu
210 215 220
Ser Leu Cys Thr Trp Ala Phe Val Arg Ser Met Lys Arg His Leu Ser
225 230 235 240
Pro Glu Trp Glu Asp Glu Gln Glu Phe Lys Met Glu Leu Tyr Gln Arg
245 250 255
Leu Pro Ser Gln Gln Ala Gln Thr Ile Leu Asp Ala Ala His Arg Pro
260 265 270
Asn Arg Ala Leu Tyr Asp Leu Ser Leu Ala Ile Glu Gln Leu Pro Met
275 280 285
His Phe Ile Arg Lys Asp Gln Met His Lys Ala Leu Thr Ile Phe Glu
290 295 300
Asp Asn Leu Gly Ser Ser Glu Arg Leu Leu Thr Ser Pro Val Pro Ile
305 310 315 320
Phe Tyr Asn Arg His Val Ala Arg Phe Leu Phe Val Trp Leu Ala Phe
325 330 335
Leu Pro Phe Ala Leu Tyr Asp Pro Phe Lys Glu Ser Trp Asn His Val
340 345 350
Met Met Ile Pro Thr Thr Ala Leu Leu Ser Leu Phe Leu Phe Gly Ile
355 360 365
Glu Glu Leu Ala Thr Gln Met Glu Glu Pro Phe Thr Ile Leu Pro Met
370 375 380
Gln Ala Phe Cys Asp Lys Ile Gly Asn Trp Cys Asn Glu Ile Val Ser
385 390 395 400
Trp Lys Asp Gly Asp Asn Gly Val Tyr Asn Lys Pro Ser Asn Asn Phe
405 410 415
Pro Ala Lys Gly His Asn Ser Val Tyr Phe Glu Ser Glu Leu
420 425 430
<210> 38
<211> 393
<212> PRT
<213> 圆柱拟脆杆藻 CCMP1102
<400> 38
Met Cys Phe Ala Phe Cys Glu Arg Leu Leu Asp Val Met Ser Ser Ser
1 5 10 15
Cys Asp Thr Ala Met Leu Leu Glu Lys Tyr Gln Val Thr Thr Lys Asp
20 25 30
Ile Pro Tyr Gly Glu Thr Ser Arg Gln Phe Arg Arg Thr Val Tyr Thr
35 40 45
His Asp Asp Trp Val Lys His Arg Ser Pro Asn Arg Phe Ile Lys Asn
50 55 60
Leu Ser Thr Ile Val Ser Ser Gly Val Tyr Lys Asn Val Gly Arg Glu
65 70 75 80
Val Ala Ser Thr Thr Ser Ile Ala Thr Phe Val Val Leu Trp Asn Met
85 90 95
Leu Thr Val Gly Tyr Asp Asp Leu Asn Gln Ile His His Leu Pro Leu
100 105 110
Ile Asn Ser Tyr Tyr Val Pro Gly Leu Thr Leu Pro Leu Gln Pro Phe
115 120 125
Thr Leu Ala Ser Gly Ser Leu Gly Leu Leu Leu Val Phe Arg Thr Asn
130 135 140
Thr Ala Tyr Gln Arg Trp Asp Glu Ala Arg Lys Asn Trp Gly Met Asn
145 150 155 160
Ile Asn His Thr Arg Asp Leu Val Arg Met Gly Asn Thr Phe Tyr Asp
165 170 175
Arg Thr Gly Val Ser Asp Glu Thr Arg Lys Glu Asp Leu Gln Arg Leu
180 185 190
Ser Val Cys Thr Trp Ser Phe Val Arg Cys Met Lys Arg His Leu Ser
195 200 205
Pro Glu Trp Glu Asp Glu Ala Ala Phe Arg Leu Glu Met Phe Glu Arg
210 215 220
Leu Pro Glu Ser Gln Ala Asn Ala Val Ile Asn Ala Ala His Arg Pro
225 230 235 240
Asn Arg Ala Leu Phe Asp Leu Ser Met Ala Ile Glu Asn Leu Pro Met
245 250 255
His Phe Met Arg Lys Asn Glu Met His Lys Ala Leu Thr Ile Phe Glu
260 265 270
Asp Asn Leu Gly Ser Ser Glu Arg Leu Leu Thr Ser Pro Val Pro Leu
275 280 285
Ile Tyr Asn Arg His Thr Leu Arg Phe Leu Ser Ile Trp Leu Leu Leu
290 295 300
Met Pro Phe Ser Leu Tyr Lys Pro Phe Asp Ser Thr Trp Asn His Ile
305 310 315 320
Gly Met Ile Pro Ala Thr Ala Met Ile Ser Leu Phe Leu Phe Gly Ile
325 330 335
Glu Glu Leu Ala Thr Gln Met Glu Glu Pro Phe Thr Ile Leu Pro Met
340 345 350
Gln Ala Phe Cys Asp Lys Ile Gly His Ser Glu Val Tyr Phe Glu Asn
355 360 365
Leu Gly Ser Ser Pro Gly Val Val Asp Gly Ser Asn Ala Phe Val Glu
370 375 380
Gln Gln Pro Glu Ile Val Arg Val Pro
385 390
<210> 39
<211> 467
<212> PRT
<213> 胸状盘藻
<400> 39
Met Leu Arg Pro Leu Pro Gly Cys Val Thr Ala Arg Arg Pro Ser Ala
1 5 10 15
Ser Cys Pro Ala Leu Trp Ser Val Glu Ala Val Leu Ser Thr Leu Arg
20 25 30
Lys Lys Arg Leu Glu Pro Asn Thr Val Glu Arg Val Tyr Glu Ala Asp
35 40 45
Val Tyr Pro Ala Val Phe Asp Phe Ala Ala Trp Thr Gln His Arg Ser
50 55 60
Arg Gly Arg Tyr Leu Glu His Cys Ile Thr Leu Phe Arg Ser Tyr Phe
65 70 75 80
Phe Arg Asp Leu Leu Gly Pro Leu Leu Val Leu Val Gly Ala Ala Val
85 90 95
Ala Val Gly Leu Tyr Glu Thr Phe Leu Gln Glu Gly Leu Leu Pro Glu
100 105 110
Trp Val Pro Asp Phe Gly Gly Val Ser Asp Thr Pro Phe Gln Leu Thr
115 120 125
Ser Phe Ala Leu Ser Leu Met Leu Val Phe Arg Thr Asn Ser Ser Tyr
130 135 140
Ala Arg Trp Leu Asp Ala Arg Gln Gln Trp Gly Leu Ile Val Asn Thr
145 150 155 160
Ala Arg Thr Phe Val Arg Gln Ala Met Thr Thr Leu Pro Glu Asp Ser
165 170 175
Cys Ser Glu Leu Arg Asp Ala Val Ala Arg Trp Thr Ile Ala Phe Val
180 185 190
Arg Leu Ser Lys Leu His Leu Arg Glu His Gly Asp Val Arg Ala Glu
195 200 205
Met Gln Gly Val Leu Arg Gly Glu Glu Leu Pro Leu Val Ala Ala Ala
210 215 220
Ala His Arg Pro Leu Ala Ala Cys His Val Leu Ser Gln Leu Met Ala
225 230 235 240
Ala Ala Ala Gly Ala Gly Ala Ile Ser Asp Gln Ser Arg Gln Arg Leu
245 250 255
Glu Ala Asp Ile Asn Gly Leu Ser Gln Ala Leu Gly Ala Cys Glu Lys
260 265 270
Ile Leu Arg Asn Pro Ile Pro Leu Ser Tyr Thr Arg His Thr Ser Arg
275 280 285
Phe Leu Ile Leu Trp Leu Leu Trp Leu Pro Val Ala Leu Trp Gly Lys
290 295 300
Ala Gly Trp Cys Val Val Pro Val Glu Ala Ile Leu Thr Tyr Leu Leu
305 310 315 320
Leu Gly Ile Asp Glu Ile Ala Ile Gln Met Glu Glu Pro Phe Gly Ile
325 330 335
Leu Pro Leu Glu Asn Phe Cys Asp Ala Val Gln Gln Ser Val Glu Gln
340 345 350
Val Thr Arg Met Asp His Ser Val His Asp Val Val Ser Ser Tyr Val
355 360 365
Cys Ala Leu Val Pro Ala Ala Pro Ala Thr Ala Ala Ala Pro Pro Val
370 375 380
Ala Ala Pro Pro Ala Thr Ala Met Gly Gly Thr Ala Ser Ala Val Val
385 390 395 400
Ala Ala Ala Ala Ala Glu Glu Ser Ser Pro Val Ala Ala Ser Val Thr
405 410 415
Ala Thr Ala Ala Pro Gly Asp Leu Asn Arg Ala Ala Arg Asp Gln Gly
420 425 430
Ser Gly Met Glu Gly Ser Gly Val Gly Asp Gly Ser Gly Asp Gly Arg
435 440 445
Thr Gly Gln Gly Phe Trp Gln Ala Ile Trp Thr Pro Asn Ile Pro Ser
450 455 460
Asp Arg Cys
465
<210> 40
<211> 481
<212> PRT
<213> 胸状盘藻
<400> 40
Met Gln Gly Arg Ala Gly Ala Ala Val Glu Glu Gly Val Ser Leu Pro
1 5 10 15
Thr Pro Thr Arg Asn Pro Val Phe Asn Pro Thr Arg Trp Ser Tyr His
20 25 30
Arg Ser Thr Ser Arg Tyr Gly Arg His Leu Thr Thr Ile Phe Lys Ser
35 40 45
Val Thr Phe Arg Asn Leu Gln Ala Pro Leu Ser Phe Leu Thr Met Met
50 55 60
Ala Cys Leu Val Val Leu Tyr Arg Thr Ser Val Glu Leu Glu Gln Leu
65 70 75 80
Pro Asp Phe Phe Glu Gly Val Thr Phe Thr Asp Val Pro Phe Gln Leu
85 90 95
Thr Ser Phe Ala Leu Ser Leu Leu Leu Val Phe Arg Thr Asp Ala Gly
100 105 110
Tyr Gly Arg Trp Cys Ala Ala Met Asp Ala Trp Gly Thr Leu Arg Asn
115 120 125
Ala Ser Ala Asp Leu Ile Arg Lys Ala Ser Ala Trp Val Asp Asp Gly
130 135 140
Pro His Leu Arg Arg Leu Val Arg Trp Val Ala Ala Phe Pro Leu Cys
145 150 155 160
Leu Met Val Glu Leu Arg His Asp Gln Gly Asp Pro Ala Met Glu Gly
165 170 175
Ala Leu Arg Ala Glu Leu Ala Arg Val Leu Arg Pro Tyr Glu Leu Gln
180 185 190
Gln Val Leu Ala Ala Gly Pro Tyr His His Gln Phe Cys Leu Ser Val
195 200 205
Leu Thr Ala Leu Val Glu Ala Ala Gln Leu Gly Glu Ser Arg Glu Thr
210 215 220
Ala Met Leu Glu Asp Leu His Gly Phe Asn Asp Ala Ala Cys Glu Cys
225 230 235 240
Glu Lys Ile Leu Arg Phe Pro Ile Pro Leu Thr Tyr Thr Arg His Thr
245 250 255
Ser Arg Phe Met Leu Ile Tyr Leu Ser Ala Leu Pro Leu Ala Leu Tyr
260 265 270
Asp Ser Cys Asp Trp Ala Val Ile Pro Val Thr Ala Val Ile Ala Phe
275 280 285
Leu Leu Leu Gly Ile Glu Asp Ile Gly Val Gln Ile Glu Glu Pro Phe
290 295 300
Ser Ile Leu Pro Leu Pro Asp Ile Cys Ala Asp Leu His Met Thr Ala
305 310 315 320
Gln Arg Ala Val Ala Gln Arg Asp Met Val Arg Cys Leu Ala Asp Pro
325 330 335
Ala Val Cys Arg Val Arg Asp Glu Asp Leu Glu Pro Thr Ala Arg Arg
340 345 350
His Ala Ala Ala His Gly Gly Pro Gly Thr Ser Tyr Gly Glu Gly Arg
355 360 365
Gly His Ala Ala Thr Gly His Pro Gly Ala Gly Pro Asp Ala Trp Gln
370 375 380
Gly Asp Glu Tyr Val Val Glu Ala Gly Phe Gly Ser Gly Gly Val Ala
385 390 395 400
Gly Gly Gly Gly Val Gly Ala Gly Ala Gly Ala Gly Ala Ser Ala Arg
405 410 415
Ser Asn Gly Asn Gly Ala Gly Gly Gly Ala Gly Gly Gly Gly Gly Lys
420 425 430
Gly Val Gly Ala Arg Gly Arg Phe Val Glu Ser Gly Lys Gly Pro Gly
435 440 445
Leu Val Ala Ala Ile Ser Glu Asn Gly Asn Gly Asn Gly Tyr Asn Gly
450 455 460
Asn Gly Asn Gly Tyr Ser Gly Gly Thr Ala Ser Gly Trp Pro His Arg
465 470 475 480
Pro
<210> 41
<211> 308
<212> PRT
<213> 胸状盘藻
<400> 41
Met Arg Val Ala Trp Ser Gly Ile Val Arg Gly Ile Ser Ala Pro Val
1 5 10 15
Leu Phe Val Thr Ala Phe Thr Ala Val Val Ala Ala Leu Asn Ala Ala
20 25 30
His Ala Ala Ser Leu Leu Pro Ala Trp Val Pro Ala Met Pro Ala Ile
35 40 45
Ala Ile Glu Pro Val Gln Leu Thr Ser Ile Ala Leu Ser Leu Leu Leu
50 55 60
Val Phe Arg Thr Asn Ala Ser Tyr Ser Arg Trp Asp Glu Gly Arg Arg
65 70 75 80
Ser Phe Gly Ser Ile Thr Thr Val Ser Arg Asp Ile Ala Arg Gln Ala
85 90 95
Phe Ala Trp Phe Arg Pro Asp Asp Gly His Asn Arg Arg Arg Leu Gly
100 105 110
Arg Trp Leu Val Ala Leu Gly Arg Ser Thr Met Val His Leu Arg Glu
115 120 125
Glu His Lys Met Glu Asp Glu Leu Lys Tyr Val Leu Glu Pro Gln Glu
130 135 140
Val Glu Ala Val Leu Ser Ser Val His Ala Pro Ser Phe Cys Leu Gln
145 150 155 160
Met Ile Thr Trp Ile Ile Arg His Ala Gly Leu Pro Gln Glu Leu Val
165 170 175
Ile Arg Met Asp Glu Asn Val Ser Arg Leu Thr Asp Ala Val Ser Ala
180 185 190
Cys Glu Arg Ile Leu Asn Thr Pro Ile Pro Leu Ser Tyr Thr Arg His
195 200 205
Thr Ala Arg Phe Leu Met Ala Trp Leu Val Cys Leu Pro Phe Cys Leu
210 215 220
Trp Pro Tyr Cys Gly Pro Ala Met Ile Pro Ile Ala Ala Leu Ile Ala
225 230 235 240
Phe Val Leu Leu Gly Ile Glu Glu Ile Gly Val Tyr Ile Glu Glu Pro
245 250 255
Phe Ser Ile Leu Ala Leu Glu Lys Leu Val Asn Lys Leu Glu Asn Ile
260 265 270
Ile Asn Ala Met Leu His Glu Trp Gln Asn Leu Asp Glu Leu Ser Arg
275 280 285
Thr Ala Met Gly Thr Ala Asn Gly Ser Gln Pro Val Ser Ala Ala Ala
290 295 300
Ser Ala Thr Ala
305
<210> 42
<211> 447
<212> PRT
<213> 胸状盘藻
<400> 42
Met Gln Leu Ser Gly Arg Gln Gln Ala Leu Ser Ala Arg Gly Val Ala
1 5 10 15
Pro Leu Pro Ser Arg Leu Cys Arg Lys Arg Leu Ile Ile Ala Ala Asn
20 25 30
Ala Ala Lys Asp Pro Asn Ala Pro Ile Gln Ser Asn Pro Leu Gly Thr
35 40 45
Leu Ser Ser Gln Gly Asn Thr Met Ala Thr Pro Pro Arg Ser Glu Ala
50 55 60
Ala Arg Lys Tyr Phe Arg Thr Val Tyr Asp Phe Pro Gln Trp Gln Glu
65 70 75 80
His Arg Ser Gln Phe Arg Leu Met Lys Arg Leu Phe Thr Ile Pro Gln
85 90 95
Ser His Val Ile Gln Asn Ala Leu Pro Ser Ile Ser Trp Val Gly Val
100 105 110
Val Ser Thr Ala Leu Thr Leu Tyr Met Thr Ala His Asp Gln His Ile
115 120 125
Leu Pro Glu Gly Phe Pro Ser Leu Val Pro Asn Ala Ser Cys Ser Ala
130 135 140
Phe Ile Ser Asn Thr Ser Val Ala Leu Ser Leu Leu Leu Val Phe Arg
145 150 155 160
Thr Asn Ser Ser Tyr Gly Arg Trp Asp Glu Ala Arg Lys Met Trp Gly
165 170 175
Gly Leu Leu Asn Arg Ser Arg Asp Ile Met Arg Gln Gly Ala Thr Cys
180 185 190
Phe Pro Asp Asp Gln Val Glu Ala Lys Lys Ala Leu Ala Arg Trp Val
195 200 205
Val Ala Phe Ala Arg Ala Leu Arg Ile His Phe Gln Pro Glu Val Thr
210 215 220
Ile Glu Ser Glu Leu Lys Asn Ile Leu Thr Pro Ala Glu Leu Glu Met
225 230 235 240
Leu Ala Lys Ser Gln His Arg Pro Val Arg Ala Ile His Ala Ile Ser
245 250 255
Gln Ile Ile Gln Ser Val Pro Met Ser Ser Ile His Gln Met Gln Met
260 265 270
Ser Asn Asn Leu Thr Phe Phe His Asp Val Leu Gly Gly Cys Glu Arg
275 280 285
Leu Leu Arg Ala Pro Ile Pro Val Ser Tyr Thr Arg His Thr Ala Arg
290 295 300
Phe Leu Phe Thr Trp Leu Ser Leu Leu Pro Phe Ala Leu Tyr Gly Gln
305 310 315 320
Cys Gly Ala Gly Val Ile Pro Val Ser Thr Gly Ile Ala Ala Val Leu
325 330 335
Cys Gly Ile Glu Glu Ile Gly Val Gln Cys Glu Glu Pro Phe Gly Ile
340 345 350
Leu Pro Leu Asp Val Ile Cys Asn Arg Ile Gln Ala Asp Val Met Ala
355 360 365
Thr Leu Lys Asp Asp Ala Asp Thr Lys Thr Ile Leu Ala Glu Ala Gly
370 375 380
Leu Val Ser Leu Leu Pro Ser Ala Ala Ser Ala Met Ala His Thr Ser
385 390 395 400
Ala Ser Ser Asn Gly Asn Gly Asn Gly Ala Asn Gly Asn Gly Ala Asn
405 410 415
Gly Asn Gly Ala Asn Gly Asn Gly Ala Ala Val Ala Ala Pro Gln Val
420 425 430
Pro Val Ser Ala Ser Ala Gly Met Thr Val Gln Ile Ser Pro Arg
435 440 445
<210> 43
<211> 1866
<212> PRT
<213> 胸状盘藻
<400> 43
Met Glu His Thr Gly Pro Ser Leu Ala Gly Pro Pro Pro Pro Pro Pro
1 5 10 15
Leu Ala Ser Ala Pro Ser Val Ala Val Glu Arg Ser Gly Ser Glu Met
20 25 30
Gly Ser Ala Leu Pro Tyr Arg Arg Arg Ser Thr Ala Pro Ala Val Ala
35 40 45
Ala Pro Pro Pro Pro Ser Val Met Val Pro Ala Ala Ala Ala Gly Pro
50 55 60
Ala Ala Ala Thr Ala Leu Ala Arg His Gln Pro Gln Ala Ala Ala Ala
65 70 75 80
Ala Gly Ala Ala Pro Phe Pro Gly Ala Ala Ala Pro Leu Pro Pro Ala
85 90 95
Leu Gln Ala Ser Thr Ser Arg Val Gly Arg Leu Leu Gln Glu Arg Phe
100 105 110
Arg Arg Cys Arg Ser His Gln Ala Phe Asn Trp Thr Pro Glu Glu Ala
115 120 125
Pro Leu Ser Pro Pro Pro Pro Pro Pro Pro Leu Ser Pro Pro Leu Pro
130 135 140
Ala Val Gln Lys Trp Ala Ala Ser Thr Val Pro Lys Pro Gly Pro Ala
145 150 155 160
Ala Ala Ala Ala Ala Ala Ala Ala Pro Pro Thr Pro Tyr Leu Ser Pro
165 170 175
Ser Pro Ser Thr Ser Pro Ser Pro Ser Pro Ser Pro Pro Pro Pro Gln
180 185 190
Ala Thr Ala Ala Ala Ser Ala Ala Ser Gly Ala Asp Gly Asp Phe Met
195 200 205
Thr Ala Ala Val Ser Glu Leu Val Glu Arg Pro Phe Arg Leu Val Val
210 215 220
Val Ala Asn Arg Leu Pro Val Thr Cys Val Gln Asp Ala Pro Gly Gly
225 230 235 240
Glu Trp Gln Leu Lys Ala Ser Ser Gly Gly Leu Val Ser Ala Leu Arg
245 250 255
Gly Leu Gly Phe Arg Ser Ser Met Trp Ile Gly Trp Pro Gly Val Trp
260 265 270
Leu Pro Pro Gly Ser Asp Arg Asn Arg Leu Thr Glu Leu Leu Arg Arg
275 280 285
Asp Gly Tyr Glu Pro Val Trp Leu Glu Ser Lys Leu Met Asp Leu Phe
290 295 300
Tyr Lys Gly Phe Cys Asn Ser Val Leu Trp Gln Leu Phe His Tyr Asp
305 310 315 320
Pro Pro Ser Leu Glu Gly Trp Ala Arg Ser Ala Glu Gln Gln Ala Ser
325 330 335
Ser Gln Ala Ala Gln Trp Gly Ala Tyr Val Glu Ala Asn Gly Arg Phe
340 345 350
Ala Asp Ala Val Leu Ala Thr His Gly Ser Ala Asp Met Val Trp Val
355 360 365
His Asp Tyr His Leu Met Leu Leu Pro Gly Ile Leu Lys Arg Ala Val
370 375 380
Pro Lys Met Arg Val Gly Phe Phe Leu His Thr Pro Phe Pro Ser Ser
385 390 395 400
Glu Val Tyr Arg Ala Leu Pro Val Arg Glu Gln Val Ser Gly Gly Gly
405 410 415
Gly Gly Gly Glu Ser Arg Leu Trp Ile Leu Ser Gly Val Leu Ala Ala
420 425 430
Asp Leu Val Gly Phe His Thr Tyr Asp Tyr Ala Arg His Phe Ile Ser
435 440 445
Ala Cys Ala Arg Ile Leu Gly Leu Glu Gly Thr Pro Glu Gly Val Glu
450 455 460
Thr His Gly Ser Leu Thr Arg Val Gly Thr Phe Pro Ile Gly Ile Glu
465 470 475 480
Thr Cys Arg Phe Val Arg Ala Ala Ala Asp Pro Asp Val Val Arg Gln
485 490 495
Val Ala Ala Leu Arg Thr Arg Phe Val Gly Arg Lys Ile Val Leu Gly
500 505 510
Val Asp Arg Leu Asp Pro Ile Lys Gly Leu Pro His Lys Leu Leu Ala
515 520 525
Phe Glu Lys Leu Leu Glu Glu Gln Pro Tyr Trp Arg Asp Lys Val Val
530 535 540
Leu Val Gln Ile Ala Val Pro Ser Arg Thr Asp Val Pro Glu Tyr Gln
545 550 555 560
His Leu Ser Ser Ile Val His Glu Ile Val Gly Arg Val Asn Gly Leu
565 570 575
Tyr Gly Ser Leu Thr His Val Pro Ile His Tyr Leu Asp Thr Cys Leu
580 585 590
Ala Phe Ser Gln Leu Val Ala Leu Tyr Gly Ala Thr Asp Val Ala Leu
595 600 605
Val Thr Ser Leu Arg Asp Gly Met Asn Leu Val Ser Tyr Glu Phe Val
610 615 620
Ala Cys Gln Gly Asp Gly Ser Gly Pro Glu Pro Pro Gly Val Leu Leu
625 630 635 640
Leu Ser Glu Phe Ala Gly Ala Ala Gln Thr His Thr Ala Gln His Trp
645 650 655
Ala Asp Thr Phe Leu Ser Glu Leu Asn Asp Thr His Ile Glu Ala Asp
660 665 670
Leu Arg Gly Arg Ala Ala Ala Pro Gln Leu Asp Val Arg Gln Val Leu
675 680 685
Glu Ala Tyr Gly Ser Ser Arg Arg Arg Leu Phe Val Leu Ser Tyr Ser
690 695 700
Ala Val Leu Thr Ala Gly Gly Glu Ala Ala Ala Gly Pro Asp Pro Ala
705 710 715 720
Asn Val Val Val Leu Met Gly Gly Cys Glu Ala Gly Arg Met Glu Ala
725 730 735
Leu Ala Gly Arg Gln Leu Ala Gly Ala Trp Leu Ala Ala Glu Asn Gly
740 745 750
Leu Leu Val Arg Pro Pro Val Pro Pro Glu Ala Leu Asp Gly Asp Asp
755 760 765
Asp Ser Gly Gly Ala Pro Ala Ala Ala Ala Thr Thr Val Pro Ala Arg
770 775 780
Gln Ala Ala Tyr Ser Pro Ser Ser Ser Ser Gly His Pro Thr Ala Ser
785 790 795 800
Gln Pro Pro Gln Pro Pro Leu Pro Arg Gly Trp Arg Trp Leu Pro Gly
805 810 815
Gly Pro Pro Ala Ser Leu Pro Pro Ser Ala Leu Pro Ser Ala Ser Pro
820 825 830
Ser Thr Ser Pro Ser Pro Arg Gly Leu Ala Ala Ala Arg Cys Val Leu
835 840 845
Asp Tyr Phe Cys Glu Arg Thr Pro Gly Ser Phe Val Glu Ala Arg Gln
850 855 860
Thr Ser Leu Val Trp Asn Tyr Lys Tyr Thr Asp Ala Glu Phe Gly Ser
865 870 875 880
Leu Gln Ala Arg Asp Leu Leu Gln His Leu Arg Ala Gly Pro Ile Gly
885 890 895
Asn Glu Pro Leu Glu Val Val Gln Ser Gly Arg Ser Val Glu Ile Arg
900 905 910
Pro Val Gly Val Thr Lys Gly Thr Ser Met Met Arg Leu Leu Gly Leu
915 920 925
Leu Ala Gly Gly Gly Gly Gly Gly Pro Pro Ser Gly Gly Gly Gly Ala
930 935 940
Ala Ala Ala Ser Thr Pro Asp Ser Gly Gly Gly Arg Ser Ala Ser Arg
945 950 955 960
Gly Tyr Gly Gly Phe Asn Gly His Gly Asn Gly Thr Ser Arg Asn Gly
965 970 975
Gly Gly Gly Gly Ser Trp Arg Arg Val Gly Ala Gly Arg Arg Arg Leu
980 985 990
Ser Ile Asp Gly Gly Arg Pro Ser Val Asp Gly Gly Arg Gly Thr Leu
995 1000 1005
Leu Pro Phe Arg Ala Gly Gly Gly Ala Gly Gly Ala Ser Gly Ala Pro
1010 1015 1020
Ala Val Gly Gly Gly Ser Asp Gly Arg Gly Gly Ser Arg Glu Val Glu
1025 1030 1035 1040
Gln Gly Ser Ser Gly Gly Asp Gly Gly Gly Gly Arg Asp Ala Ala Thr
1045 1050 1055
Ala Ala Ala Ala Ala Ala Gly Phe Asp Phe Ala Leu Val Val Cys Gly
1060 1065 1070
His Leu Thr Pro Arg Asp Glu Asp Met Phe Ile Leu Phe Arg Asp Asp
1075 1080 1085
Gly Asn Ser Gly Gly Gly Gly Cys Gly Ala Ser Ala Ser Ala Val Arg
1090 1095 1100
Ala Pro Asp Ala Ser Gly Pro Ala Asn Gly Phe Ala Ala Asn Gly Leu
1105 1110 1115 1120
Val Gln Asn Gly Gly Gly Gly Gln Ala Ile Pro His Ala Pro Ala Pro
1125 1130 1135
Ala Ser Arg Pro Gln Val Ala Glu Gly Leu Gly Ala Asn Ala Gly Gly
1140 1145 1150
Gly Gly Gly Ile Gly Gly Thr Gly Gly Gly Thr Gly Gly Gly Asp Gly
1155 1160 1165
Gly Ala Pro Gly Asn Arg Arg Arg Cys Ala Ser Phe Thr Val Ala Leu
1170 1175 1180
Gly Arg Arg Pro Ser Cys Ala Ser Ser Ser Leu Gly Gly Gly Gly Gln
1185 1190 1195 1200
Leu Val Asp Leu Leu Gln Ala Leu Ala Ala Gly Arg Ala Ala Ala Pro
1205 1210 1215
Ser Gln Gly Glu Pro Pro Gly Ala Ala Ala Gly Thr Ala Ala Pro Ile
1220 1225 1230
Ser Asp Pro Asn Asn Arg Arg Arg Gln Gly Val Val Ala Asp Ala Pro
1235 1240 1245
Pro Gln Pro His Ser His Glu Arg Trp Gln Pro Lys Ala Gly Gly Thr
1250 1255 1260
Ser Ala Ile Arg Thr Gln Arg Arg Gly Ser Asp Gly Asp Leu Gly Ala
1265 1270 1275 1280
Ala Gly Thr Ala Ala Gly Ala Ala Ala Gly Val Thr Thr Gly Asn Gly
1285 1290 1295
Ile Ala Glu Cys Thr Ala Gly Arg Arg Ser Ala Arg Val His Glu Pro
1300 1305 1310
His Thr Thr Val Arg Gln Ala Ser Glu Pro Ser Glu Gly Glu Ser Pro
1315 1320 1325
Phe Thr Ala Asp Gly Ala Gly Gly Gly Gly Arg Ser Gly Ser Asp Ala
1330 1335 1340
Asp Ala Asp Ala Asp Ala Pro Asp Ala Asn Asp Gly Asp Gly Asp Gly
1345 1350 1355 1360
Gly Asp Gly Asp Gly Pro Leu Arg Pro Leu Ala Glu Pro Leu Ser Ala
1365 1370 1375
Phe Pro Ser Pro Val Leu Pro Ala Ala Ala Ser Pro Leu Ala Phe Gly
1380 1385 1390
Gly Thr Pro Leu Gln Ala Phe Ala Arg Trp Ala Ala Ala Pro Ala Ala
1395 1400 1405
Ala Gly Ala Ala Ala Val His Ser Gly Ser Ser Asn Ser Gly Gly Gly
1410 1415 1420
Arg Ser Gly Gly Arg Gly Phe Gly Ser Gly Ala Ala Gly Gly Gly Gly
1425 1430 1435 1440
Gly Gly Gly Cys Val Ala Arg Arg Ser Leu Val Val Val Ala Asn Ala
1445 1450 1455
Ala Lys Asp Pro Asn Ala Pro Ile Gln Ser Asn Pro Leu Gly Thr Leu
1460 1465 1470
Ser Ser Gln Ser Gly Thr Val Ser Val Leu Pro Arg Ser Glu Glu Ala
1475 1480 1485
Arg Arg Tyr Trp Arg Thr Val Tyr Asp Phe Pro Gln Trp Gln Lys His
1490 1495 1500
Arg Ser Ser Tyr Arg Phe Ala Glu Arg Leu Phe Gln Leu Ser Gln Ser
1505 1510 1515 1520
His Ile Leu Gln Asn Ala Leu Pro Ser Ile Ser Trp Val Thr Leu Val
1525 1530 1535
Ala Thr Ala Val Ala Ala Tyr Gly Thr Ala Tyr Asp Ala Glu Met Leu
1540 1545 1550
Pro Glu Ile Leu Pro Ser Ile Ala Pro Asn Ala Ser Cys Thr Ala Phe
1555 1560 1565
Ile Ser Asn Thr Ser Val Ala Leu Ser Leu Leu Leu Val Phe Arg Thr
1570 1575 1580
Asn Ser Ser Tyr Gly Arg Trp Asp Glu Ala Arg Lys Met Trp Gly Gly
1585 1590 1595 1600
Leu Leu Asn Arg Ser Arg Asp Ile Met Arg Gln Gly Ala Thr Cys Phe
1605 1610 1615
Pro Asp Asp Gln Val Glu Ala Lys Lys Ala Leu Ala Arg Trp Val Val
1620 1625 1630
Ala Phe Ala Arg Ala Leu Arg Ile His Phe Gln Pro Glu Val Thr Leu
1635 1640 1645
Glu Ser Glu Leu Lys Asn Ile Leu Thr Pro Ala Glu Leu Glu Met Leu
1650 1655 1660
Ala Lys Ser Gln His Arg Pro Val Arg Ala Ile His Ala Ile Ser Gln
1665 1670 1675 1680
Ile Ile Gln Ser Val Pro Met Ser Ser Ile His Gln Met Gln Met Ser
1685 1690 1695
Asn Asn Leu Thr Phe Tyr His Asp Val Leu Gly Gly Cys Glu Arg Leu
1700 1705 1710
Leu Arg Ala Pro Ile Pro Val Ser Tyr Thr Arg His Thr Ala Arg Phe
1715 1720 1725
Leu Phe Ala Trp Leu Thr Leu Leu Pro Phe Ala Leu Tyr Pro Thr Val
1730 1735 1740
Gly Trp Gly Val Val Pro Val Cys Thr Gly Ile Ala Ala Val Leu Cys
1745 1750 1755 1760
Gly Ile Glu Glu Ile Gly Val Gln Cys Glu Glu Pro Phe Gly Ile Leu
1765 1770 1775
Pro Leu Asp Val Ile Cys Asn Arg Ile Gln Ala Asp Val Met Ala Thr
1780 1785 1790
Leu Lys Asp Asp Ala Asp Thr Lys Thr Val Leu Ala Glu Ala Gly Leu
1795 1800 1805
Leu Asn Leu Val Pro Ser Ala Ala Ala Ala Val Pro Val Ala Ser Ala
1810 1815 1820
Ala Pro Ala Ala Pro Ala Ala Ala Gln Gln Pro Ala Met Ala Gly Ser
1825 1830 1835 1840
Ala Ser Ser Ser Ser Ala Gly Arg Gly Pro Ser Asn Gly Leu Gln Val
1845 1850 1855
Arg Val Ala Ile Gly Ser Gln Asp Lys Gln
1860 1865
<210> 44
<211> 381
<212> PRT
<213> 克雷伯氏菌
<400> 44
Met Asn Ala Pro Lys Ile Asp Pro Glu Pro Leu Leu Glu Tyr Leu Gln
1 5 10 15
Asp Glu Thr Lys Lys Thr Ile Phe Asp Phe Val Arg Trp Ala Thr His
20 25 30
Arg Asn Thr Arg Arg Tyr Ala Arg His Val Ser Gly Leu Trp Arg Ser
35 40 45
Gly Ile Ile Arg Ser Leu Arg Ser Pro Leu Leu Phe Val Phe Phe Ser
50 55 60
Ser Leu Ser Leu Val Leu Tyr His Thr Ala Lys Asp Arg Ala Trp Ile
65 70 75 80
Ser Pro Ser Phe Pro Thr Leu Ser Leu Ser Ser Thr Gln Pro Phe Thr
85 90 95
Leu Ser Ser Leu Ala Leu Ser Leu Leu Leu Val Phe Arg Thr Asn Ala
100 105 110
Ser Tyr Ala Arg Phe Asp Glu Ala Arg Thr Ile Leu Gly Ala Ile Leu
115 120 125
Cys Arg Thr Arg His Leu Ala Arg Gln Gly Leu Ala Trp Ile Asp Pro
130 135 140
Lys Asp Ala Asp Leu Ile Ala Leu Leu Glu Arg Trp Thr Ile Ala Phe
145 150 155 160
Thr Phe Ala Leu Ala Val His Leu Arg Pro Asp Gly Asp Val Arg Lys
165 170 175
Glu Leu Gln Gly Val Leu Leu Pro Ser Glu Leu Ala Ala Leu Glu Glu
180 185 190
Ala Glu His Lys Pro Ser Phe Val Leu Ser Val Leu Gln His Ile Leu
195 200 205
Pro Gln Ala Gly Val Ser Asp Tyr Leu Gln Gly Cys Met Asn Glu Ser
210 215 220
Leu Ala Gln Leu Glu Thr Ser Leu Gly Ser Cys Glu Arg Ile Phe Asn
225 230 235 240
Thr Pro Ile Pro Leu Met Tyr Thr Arg His Thr Ser Arg Phe Leu Met
245 250 255
Leu Trp Ile Gly Ile Ile Pro Phe Met Met Trp Glu Ala Cys Gly Phe
260 265 270
Ala Ser Leu Ile Leu Ser Pro Ala Ile Ala Phe Phe Leu Leu Gly Ile
275 280 285
Glu Glu Ile Gly Val Gln Ile Glu Glu Pro Phe Ser Ile Leu Pro Ile
290 295 300
Thr Tyr Ile Ala Ser Val Ala Glu Arg Asn Ile Arg Glu Met Val Lys
305 310 315 320
Lys Arg Glu Leu Thr Arg Gly Ile Val Gly Arg Gly Arg Arg Gly Val
325 330 335
Ser Trp Glu Gly Gly Leu Gly Gly Pro Leu Asp Gly Val Met Asn Gly
340 345 350
Cys Asn Ala Val Ser Gly Gln Gln Ser Glu Gly His Phe Glu Arg Ala
355 360 365
Gln Lys Arg Cys Leu Phe Arg Thr His Phe Ser Ser Leu
370 375 380
<210> 45
<211> 460
<212> PRT
<213> 克雷伯氏菌
<400> 45
Met Ala Thr Ser Ala His Val Ala Met Arg Pro Gln Ala Leu Trp Thr
1 5 10 15
Gly Gly Gly Pro Ala Ser Ala Arg Ser Ala Pro Ala Phe Asn Pro Leu
20 25 30
Pro Ala Gln Val Gly Cys Pro Leu Ala Thr Lys Glu Lys Trp Gln Arg
35 40 45
Met Trp Gly His Ala Ser Leu Arg Ala Ser Leu Asp Leu Ala Asp Ser
50 55 60
Lys Glu Glu Ala Ala Arg Lys Thr Arg Thr Pro Ser Leu Pro Gln Arg
65 70 75 80
Arg Ser Val Arg Cys Gln Ser Ala Pro Lys Ser Asn Ser Pro Pro Pro
85 90 95
Val Ala Leu Lys His Tyr Leu Thr Pro Ile Ser Ala Asp Val Lys Lys
100 105 110
Glu Glu Ser Met Val Tyr Arg Arg Thr Val Phe Gly His Pro Glu Trp
115 120 125
Leu Arg His Arg Ser Ser Thr Arg His Tyr Arg His Ile Leu Ser Ile
130 135 140
Ser Ser Ser Arg Val Ile Leu Ala Leu Gly Pro Pro Val Leu Ala Leu
145 150 155 160
Thr Gly Gly Ala Leu Ala Met Ala Leu Tyr Asn Glu Ala Ile Val His
165 170 175
Asp Ile Leu Pro His Glu Leu Pro Leu Leu Lys Ala Ser Ala Leu Pro
180 185 190
Leu Gln Leu Thr Ala Pro Ala Leu Ala Leu Leu Leu Val Phe Arg Thr
195 200 205
Asn Ala Ser Tyr Ser Arg Phe Asp Glu Ala Arg Lys Met Trp Gly Ala
210 215 220
Thr Leu Asn Arg Val Arg Asp Leu Ala Arg Gln Ala Leu Ser Tyr Met
225 230 235 240
Asp Asp Asn Glu Glu Arg Glu Glu Val Leu Arg Tyr Leu Met Ala Phe
245 250 255
Pro Tyr Ala Phe Lys Asn His Leu Leu Arg Glu Asp His Ile Val Asp
260 265 270
Asp Met Arg Asn Val Gly Leu Asn Ser Asp Glu Ile Glu Ile Leu Thr
275 280 285
Ser Thr Glu His Arg Pro Asn Tyr Cys Leu Gln Val Met Ser Gln Val
290 295 300
Ile Ser Lys Asn Thr Asp Leu Thr Asp Phe Gln Arg Val Asn Leu Asp
305 310 315 320
His Asn Leu Thr His Phe His Asp Gln Val Gly Gly Cys Glu Arg Ile
325 330 335
Phe Lys Thr Pro Leu Pro Leu Ser Tyr Thr Arg Leu Thr Ser Arg Phe
340 345 350
Leu Val Leu Trp His Phe Phe Leu Pro Leu Ala Leu Trp Asp Ala Cys
355 360 365
His Trp Met Val Val Pro Ala Thr Phe Leu Ser Ala Ala Ala Leu Phe
370 375 380
Cys Ile Glu Glu Val Gly Val Leu Ile Glu Glu Pro Phe Ser Ile Leu
385 390 395 400
Pro Leu His Ala Ile Cys Asn Thr Ala Glu Lys Asn Val Arg Glu Leu
405 410 415
Thr Arg Leu Gln Gly Glu His Leu Asn Lys Arg Ala Ile Gly Gly Lys
420 425 430
Lys Gln Ala Gln Leu Asn Gly Ala Ser Gln Asn Gly Val Ala Asp Gly
435 440 445
Ala Ala Ala Arg Arg Thr Asn Val Gln Val Thr Asn
450 455 460
<210> 46
<211> 428
<212> PRT
<213> 地钱
<400> 46
Met Glu Thr Leu Cys Ser Ile Pro Lys Leu Thr Ser Arg Cys Leu Ser
1 5 10 15
Phe Ser Thr Ser Leu Leu Glu Leu Ser Arg Phe His Ser Phe Cys Pro
20 25 30
Gly Leu Pro Gln Glu Leu Asn Val Arg Arg Gly Arg Thr Ala Phe Arg
35 40 45
Leu Arg Ala Gln Pro Cys Ser Gly Ile Ser Thr Ile His Val Lys Glu
50 55 60
Ser Arg Val Ser Arg Arg Gln Thr Ile Arg Cys Val Gly Ser Gly Pro
65 70 75 80
Glu Pro Glu Pro Tyr Asp Pro Asp Phe Pro Lys Glu Glu Val Phe Leu
85 90 95
Tyr Arg Arg Thr Val Tyr Asp His Lys Asp Trp Ser Arg His Arg Ser
100 105 110
Ser Leu Arg His Ser Arg His Ile Leu Ser Met Arg Ser Ser Arg Val
115 120 125
Ile Leu Ala Leu Trp Pro Pro Val Phe Gly Leu Thr Thr Val Ser Ile
130 135 140
Ala Leu Ser Ala Tyr Asn Glu Cys Ile Leu Ser His Trp Leu Pro Ser
145 150 155 160
Phe Leu Pro Leu Leu His Val Ser Ala Thr Pro Phe Gln Leu Met Ala
165 170 175
Pro Ala Leu Ala Leu Leu Leu Val Phe Arg Thr Asn Ala Ser Tyr Ala
180 185 190
Arg Phe Asp Glu Ala Arg Arg Ala Trp Gly Ser Asn Val Asn Arg Thr
195 200 205
Arg Asp Ile Thr Arg Gln Ala Leu Thr Trp Met Gln His Pro Ser Asp
210 215 220
Ala Glu Lys Val Lys Lys Leu Ile Arg His Cys Val Ala Phe Asn Val
225 230 235 240
Cys Met Lys His His Leu Val Arg Gly Gly Asp Leu Arg Asp Asp Leu
245 250 255
His Ser Trp Ile Asp Lys Glu Glu Ile Asp Gly Ile Leu Ala Ser Thr
260 265 270
His Arg Pro Asn Tyr Val Leu Gln Val Met Ser Glu Ile Ile His Ser
275 280 285
Cys Ser Ile Thr Glu Met Gln Leu Thr Arg Met Asp Val Asn Met Thr
290 295 300
Gln Phe Ala Asp Asn Leu Gly Ala Cys Glu Arg Ile Phe Lys Thr Pro
305 310 315 320
Ile Pro Leu Ser Tyr Thr Arg Leu Thr Ser Arg Phe Leu Val Leu Trp
325 330 335
His Ile Ala Leu Pro Leu Ala Leu Trp Asp His Cys Gln Trp Val Ser
340 345 350
Val Pro Ala Thr Phe Leu Ser Ala Gly Ala Leu Phe Cys Ile Glu Glu
355 360 365
Val Gly Val Leu Ile Glu Glu Pro Phe Gln Ile Phe Pro Leu Asp Asn
370 375 380
Ile Cys Ser Thr Ile Lys Lys Asn Val Asp Gly Leu Ile Leu Ala His
385 390 395 400
Thr Glu Ile His His Cys His Lys His Pro Pro Lys Gly Cys Asp Ile
405 410 415
Pro Lys Lys Lys Ser Ser Gly Asp Ala Pro Lys Ser
420 425
<210> 47
<211> 337
<212> PRT
<213> Monoraphidium neglectum
<400> 47
Met Gly Ala Ala Ala Ala Val Val Ala Tyr Glu Ala Leu Arg Thr Gly
1 5 10 15
Ala Leu Thr Phe Leu Pro Ser Leu His Val Ser Pro Gln Leu Ile Ser
20 25 30
Leu Thr Ser Phe Ala Leu Ser Leu Leu Leu Val Phe Arg Thr Asn Ala
35 40 45
Ser Tyr Glu Arg Trp Asp Gly Ala Arg Lys Thr Trp Gly Leu Ile Ile
50 55 60
Asn Arg Ser Arg Asp Ile Val Arg Gln Gly Leu Thr His Ile Pro Arg
65 70 75 80
Asp Lys Pro Asp Leu Arg Ala Met Leu Cys Arg Trp Val Pro Ala Phe
85 90 95
Ser Lys Ala Leu Met Cys His Leu Arg Arg Asp Lys Ser Leu Arg Gln
100 105 110
Glu Leu Gln Gly Val Leu Leu Pro Ala Glu Val Asp Glu Val Met Arg
115 120 125
Ser Gln His Arg Pro Asn Tyr Val Leu Gln Val Leu Ala Glu Ile Ile
130 135 140
Arg Thr Gly Arg Val Arg Ala Pro Ala Ala Ile Arg Met Glu Glu Asn
145 150 155 160
Leu Thr Ser Phe Glu Asp Cys Leu Gly Gly Cys Glu Arg Val Leu Lys
165 170 175
Thr Pro Ile Pro Leu Ser Tyr Thr Arg His Thr Ser Arg Ser Leu Met
180 185 190
Ile Trp Leu Thr Leu Leu Pro Phe Ala Leu Val Ser Thr Cys Gly Leu
195 200 205
Ala Thr Ile Pro Leu Cys Gly Phe Val Ala Phe Leu Leu Leu Gly Glu
210 215 220
Thr Ile Arg Ile Glu Glu Ile Gly Val Ser Ile Glu Glu Pro Phe Ser
225 230 235 240
Thr Leu Pro Leu Ala Ala Ile Cys Gly Ser Val Leu Thr Asn Val Arg
245 250 255
Glu Leu Gln Ala Thr His Ala Ala Asp Thr Thr Gly Ser Ser Ala Ala
260 265 270
Asp Val Asn Pro Leu Pro Leu Ser Ala Ser Gln Leu Val Thr Arg Tyr
275 280 285
Gly Val Ala Ala Ala Glu Ala Glu Val Arg Gly Met Asp Val Ala Val
290 295 300
Ser Gly Ala Ala Ala Ala Ala Ala Ala Ile Arg Ala Gly Val Pro Gly
305 310 315 320
Asn His Ala Val Trp Pro Gln Ile Arg Ala Gly Leu Ala Ala Ala Gln
325 330 335
Ser
<210> 48
<211> 430
<212> PRT
<213> Monoraphidium neglectum
<400> 48
Met Gln His Ala Ala Arg Leu Gln His Ala Ala Ala Ala Pro Gln Leu
1 5 10 15
Met Arg Pro Pro Thr His Gln Arg Ala Leu Cys Ala Thr Ile Ser Ala
20 25 30
Tyr Val Arg Ser Ile Glu Lys Gly Asp Asp Leu Ala Lys Asn Gln Leu
35 40 45
Cys His Val Lys Arg Ala Ser Val Arg Ala Lys Ala Ser Ala Lys Asp
50 55 60
Pro Arg Val Phe Asp His Asp Asn Trp Asp Val His Arg Ser Thr Thr
65 70 75 80
Arg His Ala Arg His Ile Ala Gly Leu Cys Arg Ser Gln Thr Val Ala
85 90 95
Asn Leu Val Arg Pro Leu Ser Tyr Val Met Gly Val Ala Val Thr Val
100 105 110
Val Ala Val Glu Ala Leu Arg Ala Ala Gly Cys Ala Pro Ala Phe Leu
115 120 125
Pro Ser Leu Gln Val Asn Ser Ala Leu Phe Gly Leu Thr Ser Phe Ala
130 135 140
Leu Ser Leu Leu Leu Val Phe Arg Thr Asn Ala Ser Tyr Glu Arg Trp
145 150 155 160
Asp Ser Ala Arg Lys Met Trp Gly Leu Val Leu Asn Arg Ser Arg Asp
165 170 175
Ile Val Arg Gln Gly Leu Thr His Ile Pro Arg Asp Lys Pro Asp Leu
180 185 190
Arg Ala Met Leu Cys Arg Trp Val Pro Ala Phe Ser Lys Ala Leu Met
195 200 205
Cys His Leu Arg Arg Asp Lys Asn Leu Ser Gln Glu Leu Gln Gly Val
210 215 220
Leu Leu Pro Ala Glu Val Asp Glu Val Met Arg Ala Gln His Arg Pro
225 230 235 240
Asn Tyr Val Leu Gln Val Leu Ala Glu Ile Ile Arg Thr Gly Arg Val
245 250 255
Arg Ala Thr Ala Ala Ile Arg Met Glu Glu Asn Leu Thr Ser Phe Glu
260 265 270
Asp Cys Leu Gly Gly Cys Glu Arg Ile Leu Lys Thr Pro Ile Pro Leu
275 280 285
Ser Tyr Thr Arg His Thr Ser Arg Thr Met Met Ile Trp Leu Thr Leu
290 295 300
Leu Pro Ser Ala Leu Val Ser Thr Cys Gly Leu Ala Thr Ile Pro Leu
305 310 315 320
Cys Gly Phe Val Ala Phe Leu Leu Leu Gly Ile Glu Glu Ile Gly Val
325 330 335
Ser Ile Glu Glu Pro Phe Ser Ile Leu Pro Leu Asp Ala Ile Ser Gly
340 345 350
Ser Val Leu Thr Asn Val Arg Glu Leu Gln Ala Thr His Asp Ala Ala
355 360 365
Ala Gly Ala Thr Gly Ala Ala Asp Ala Ala Pro Leu Pro Leu Ser Ala
370 375 380
Ser Gln Leu Val Thr Arg Tyr Gly Val Ala Ala Ala Glu Ala Gln Val
385 390 395 400
Arg Gly Met His Val Gly Val Asn Gly Ala Ala Ala Ala Val Ala Ala
405 410 415
Asn Gly Thr Ala Val Arg Ser Thr His Ala Val Trp Pro Gln
420 425 430
<210> 49
<211> 318
<212> PRT
<213> Monoraphidium neglectum
<400> 49
Met Thr Ser Pro Ile His Ser Tyr Gly Arg Trp Asp Glu Ala Arg Lys
1 5 10 15
Met Trp Gly Leu Val Leu Asn Arg Ser Arg Asp Ile Ala Arg Gln Gly
20 25 30
Leu Ser Tyr Ile Pro Asp Asp Arg Gln Asp Leu Arg Asp Met Leu Cys
35 40 45
Arg Trp Thr Pro Ala Phe Ser Arg Ser Leu Met Thr His Leu Arg Lys
50 55 60
Gly Glu Asp Leu Arg Glu Glu Leu Lys Asp Ile Leu Arg Pro His Glu
65 70 75 80
Leu Asp Ser Leu Leu Met Ser Thr His Arg Pro Asn Tyr Cys Leu Gln
85 90 95
Val Leu Ser Gln Val Val Lys Asp Ala Asn Ala Gly Thr Ala Ala Thr
100 105 110
Ile Arg Met Asp Asp Asn Ile Thr Ala Phe Glu Asp Cys Leu Gly Gly
115 120 125
Cys Glu Arg Ile Leu Lys Thr Pro Val Pro Leu Ser Tyr Thr Arg His
130 135 140
Thr Ser Arg Phe Leu Ile Ile Trp Leu Thr Met Leu Pro Phe Thr Leu
145 150 155 160
Tyr Gly Ser Cys Gly Phe Ala Thr Val Pro Leu Cys Cys Val Ile Ala
165 170 175
Phe Leu Leu Leu Gly Ile Glu Glu Ile Gly Val Ser Ile Glu Glu Pro
180 185 190
Phe Ser Ile Leu Ala Leu Gln Ala Ile Cys Asp Ser Ala Leu Asn Asn
195 200 205
Val Arg Glu Leu Gln Ala Gln His Asp Ile Ser Lys Ala Lys Ser Asp
210 215 220
Gln Tyr Ser Ala Ile Glu Leu Val Ser Met Ala Ala Ala Glu Ala Ala
225 230 235 240
Gly Ala Ser Ala Thr Ala Ala Ala Ala Glu Ser His Asn Gly Asn Gly
245 250 255
Asn Gly Asn Gly Ser Arg Ala Pro Ser Pro Ala Phe Ala Thr Ala Gly
260 265 270
Val Ala Asn Gly Gly Asn Arg Phe Ala Val Ala Pro Pro Gly Ser Asn
275 280 285
Gly Thr Ala Ala Ser Ser Asp Gly Ala Gly Ser Gly Gly Ser Asn Val
290 295 300
Trp Pro Arg Lys Gly Asn Tyr Ser Ser Val Ser Phe Arg Lys
305 310 315
<210> 50
<211> 363
<212> PRT
<213> Monoraphidium neglectum
<400> 50
Met Glu Leu Arg Cys Ala Pro Ala Ser Ala Pro Gly Ile Ala Pro Asn
1 5 10 15
Asp Thr Ala Ala Ala Ala Ala Ser Phe Gly Gly Ala Pro Ile Ser His
20 25 30
Gly Gly Ala Ala Ala Ala Asn Thr Thr Lys Gly Gly Arg Glu Gln Ser
35 40 45
Val Gly Gly Thr Ala Arg Pro Arg Ser Ala Leu Gly Arg Leu Ser Arg
50 55 60
Ser Lys Ile Leu Asn Leu Gly Ala Pro Glu Phe Val Lys Tyr Val Leu
65 70 75 80
Asp Lys Asp Gly His Asp Arg His Glu Thr Asp Ile Thr Arg Arg Arg
85 90 95
Phe Trp His Asn Val Arg Arg Arg Glu Ala Gly Met Arg Gly Lys Gln
100 105 110
Thr Ser Lys Phe Phe Ser Ala Ala Gly Ser Gly Val Leu Ala Pro Ala
115 120 125
Asp Leu Val Phe Thr Phe Asp Arg Trp Asn Lys His Arg Asn Ser Leu
130 135 140
Arg Tyr Val Lys His Leu Thr His Val Phe Thr Ser Arg Ile Phe Arg
145 150 155 160
Asn Leu Arg Gly Pro Val Val Val Val Met Ala Met Ala Val Leu Val
165 170 175
Gly Thr Tyr Glu Thr Leu Arg Thr Thr Gly Tyr Leu Pro Gly Phe Pro
180 185 190
His Leu Ala His Ser Ala Leu Asp Glu Gly Phe Gln Leu Thr Ala Phe
195 200 205
Ala Leu Ser Leu Leu Leu Val Phe Arg Thr Asn Ser Ser Tyr Asp Arg
210 215 220
Trp Trp Glu Ala Arg Lys Ile Trp Gly Gly Val Val Asn Arg Ser Arg
225 230 235 240
Asp Val Val Arg Gln Gly Leu Val Phe Phe Ala Asp Glu Asp Thr His
245 250 255
Leu Lys Glu Thr Leu Ala Cys Trp Ala Met Ala Phe Pro Lys Val Leu
260 265 270
Met Cys His Leu Arg Asp Ser Met Asp Val Lys Lys Glu Val Gly His
275 280 285
Leu Leu Ser Pro Leu Glu Leu Glu Leu Leu Cys Ser Ser Ala His Arg
290 295 300
Pro Asn Phe Ile Leu Gln Val Ile Ser Glu Thr Ile Arg Ala Ala Lys
305 310 315 320
Pro His Glu Leu Tyr Arg Ile Arg Met Asp Glu Asn Leu Thr Phe Phe
325 330 335
His Asp Ala Leu Gly Ser Cys Glu Arg Ile Leu Arg Thr Pro Ile Pro
340 345 350
Leu Ser Tyr Thr Arg His Thr Ser Arg Phe Leu
355 360
<210> 51
<211> 406
<212> PRT
<213> Monoraphidium neglectum
<400> 51
Met Met Val Cys Gly Leu Met Ala Gly Arg Val Gln Leu Thr Asp Leu
1 5 10 15
Gly Arg Pro Val Ala Gly Asp Tyr Glu Asp Ala Ile Lys Glu Glu Ser
20 25 30
Arg Arg Tyr Arg Arg Thr Val Phe Asp Ile Gln Ala Trp Glu Gly His
35 40 45
Arg Ser Thr Thr Arg Tyr Gly Arg His Leu Val Gly Ile Leu Thr Ser
50 55 60
Arg Val Val Trp Gly Leu Ala Arg Pro Leu Leu Tyr Val Ala Ala Val
65 70 75 80
Ala Ala Ala Val Val Ala Tyr Glu Ser Leu Arg Gln Ala Gly Leu Leu
85 90 95
Pro Ala Ala Leu Pro Ser Val Gln Leu Thr Ser Lys Glu Pro Phe Gly
100 105 110
Leu Thr Ser Phe Ala Leu Ser Leu Leu Leu Val Phe Arg Thr Asn Ser
115 120 125
Ser Tyr Gly Arg Trp Asp Glu Ala Arg Lys Met Trp Gly Leu Val Leu
130 135 140
Asn Arg Ser Arg Asp Ile Val Arg Gln Gly Leu Ser Tyr Ile Ser Pro
145 150 155 160
Asp Cys Trp Gln Leu Arg Asp Met Leu Cys Arg Trp Thr Pro Ala Phe
165 170 175
Ser Arg Ala Leu Met Cys His Leu Arg Lys Gly Asn Asp Leu Arg Gln
180 185 190
Glu Leu Gln Asp Ile Leu Leu Pro His Glu Leu Asp Ser Leu Leu Lys
195 200 205
Ala Thr His Arg Pro Asn Tyr Val Leu Gln Val Leu Ser Gln Ile Ile
210 215 220
Ser Ser Ala Asn Leu Ser Val Ala Ala Thr Ile Arg Met Asp Gln Asn
225 230 235 240
Leu Thr Ser Phe Ala Asp Cys Leu Gly Gly Cys Glu Arg Ile Leu Arg
245 250 255
Thr Pro Ile Pro Leu Ser Tyr Thr Arg His Thr Ser Arg Phe Leu Met
260 265 270
Ile Trp Leu Thr Leu Leu Pro Phe Ser Leu Phe Gly Ser Cys Gly Leu
275 280 285
Ser Thr Ile Pro Leu Ser Val Ala Ile Ala Phe Leu Leu Leu Gly Ile
290 295 300
Glu Glu Ile Gly Val Ser Ile Glu Ala Glu Pro Phe Ser Ile Leu Ala
305 310 315 320
Leu Glu Val Ile Cys Asp Ser Val Leu Ser Asn Val Arg Glu Ile Gln
325 330 335
Ser Thr His Ala Ala Asn Gly Asn Gly Gly Ser Ser Thr Gly Val Ser
340 345 350
Gly Asn Gly Asn Gly Ser Gly Ala Pro Pro Ala Gln Gln Arg Arg Glu
355 360 365
Gln Leu Ser Ala Ala Gln Leu Val Gly Leu Ala Arg Ser Gly Pro Gln
370 375 380
Gly Gln Lys Glu Trp Pro Pro Val Gln Ala Val Asn Arg Tyr Pro Val
385 390 395 400
Pro Gln Ala Ala Gly Val
405
<210> 52
<211> 358
<212> PRT
<213> 小立碗藓
<400> 52
Met His Arg Asn Ala Val Tyr Gly His Asp Glu Trp Ala Lys His Lys
1 5 10 15
Ser Cys Trp Arg His Gly Arg His Leu Lys Thr Ile Phe Ala Ser Arg
20 25 30
Pro Ile Val Ala Thr Gly Pro Pro Val Ala Phe Cys Thr Leu Ile Ala
35 40 45
Val Phe Val Val Ile Phe Asn His Ser Val Leu Val Gly His Phe Pro
50 55 60
Val Trp Val Pro Val Ile Gln Val Ala Ser Ile Pro Phe Ala Leu Thr
65 70 75 80
Ser Ser Val Leu Ser Leu Leu Leu Val Phe Arg Thr Asn Ser Ser Tyr
85 90 95
Asn Arg Phe Asp Glu Ala Arg Lys Ala Trp Gly Ser Asn Val Asn Arg
100 105 110
Thr Arg Asp Leu Ala Arg Gln Ala Leu Thr Trp Ile Arg Ser Pro Ala
115 120 125
Asp Leu Pro Lys Leu His Cys Leu Leu Arg His Ile Lys Ala Tyr Ser
130 135 140
Tyr Cys Leu Lys Asp His Leu Thr Gln Asp Asn Thr Leu Arg Glu Glu
145 150 155 160
Leu Ala Lys Val Leu Glu Pro Thr Glu Leu Glu Leu Val Leu Ser Ser
165 170 175
Lys His Arg Pro Asn Tyr Val Met Gln Val Met Ser Glu Leu Ile Lys
180 185 190
Gln Cys Lys Val Ser Glu Trp Glu Ser Met Ser Met Asp Arg Asn Leu
195 200 205
Thr Gln Phe His Asp Asn Val Gly Ala Cys Glu Arg Leu Phe Lys Thr
210 215 220
Pro Ile Pro Val Ala Tyr Thr Arg Leu Thr Ser Arg Val Leu Ser Leu
225 230 235 240
Trp His Ile Ser Leu Pro Phe Ala Leu Trp Asn Ser Cys His Trp Leu
245 250 255
Thr Ile Pro Ala Thr Phe Phe Ser Ser Ala Ala Leu Phe Tyr Ile Glu
260 265 270
Glu Val Gly Val Leu Ile Glu Glu Pro Phe Trp Ile Leu Ala Leu Met
275 280 285
Ser Ile Ser Asp Gly Ile Cys Ser Ala Leu Asp Gly Leu Val Ala Ala
290 295 300
His Gln Glu Thr Arg Leu Val Trp Gly Val His Pro Gln Ala Val Cys
305 310 315 320
Lys Lys Asp His Val Val Ile Thr Phe Glu Asn Ser Arg Ser Cys Ala
325 330 335
Ser Asp Gly Phe Arg Pro Gln His Asp Tyr Ile Asp Val Ala Met Thr
340 345 350
Arg Val Asn Ser Val Ser
355
<210> 53
<211> 416
<212> PRT
<213> 小立碗藓
<400> 53
Met Ala Pro Gly Gln Val His Thr Asp Cys Leu Trp Met Lys Tyr Ser
1 5 10 15
Cys Leu Arg Asn Thr Tyr Ser Lys Leu Gln Ala Leu Gln Met Lys Asp
20 25 30
Leu Asp Phe Arg His Trp Ala Ser Gln Glu Phe Ser Leu Val Thr Pro
35 40 45
Glu Glu Gln Ala Val Leu Gly Ser Gly Leu Glu Pro Gln Lys Ile Met
50 55 60
His Arg Asn Lys Val Tyr Gly His Leu Glu Trp Ala Arg His Lys Ser
65 70 75 80
Ser Trp Arg His Gly Arg His Ile Phe Ser Ile Leu Ser Ser Gly Val
85 90 95
Ile Phe Ala Val Phe Pro Pro Val Leu Val Cys Thr Leu Phe Gly Thr
100 105 110
Phe Val Thr Ile Phe Asn His Phe Val Gln Asn Gly His Leu Pro Thr
115 120 125
Trp Met Pro Ile Leu His Val Ala Ser Leu Pro Phe Thr Leu Thr Ser
130 135 140
Ser Val Leu Ser Leu Leu Leu Val Phe Arg Thr Asn Ser Ser Tyr Asn
145 150 155 160
Arg Phe Glu Glu Ala Arg Lys Phe Trp Gly Ser Asn Val Asn Arg Thr
165 170 175
Arg Asp Leu Val Arg Gln Ser Leu Thr Trp Ile Ser Gln Pro Gly Asp
180 185 190
Ser Leu Ile Leu Leu Ser Leu Leu Arg His Ile Lys Ala Tyr Ser Phe
195 200 205
Cys Leu Lys Asp His Leu Thr Glu Asp Glu Thr Leu Arg Asp Asp Leu
210 215 220
Val Gly Ile Val Glu Pro His Glu Leu Glu Ser Ile Leu Ser Ser Pro
225 230 235 240
His Arg Pro Asn Tyr Ile Leu Gln Val Leu Ser Glu Leu Ile Asn Gln
245 250 255
Cys His Ile Ser Gln Trp Glu Lys Met Ser Met Asp Glu Asn Ile Thr
260 265 270
Thr Phe His Asp Asn Val Gly Ala Cys Glu Arg Ile Leu Lys Thr Pro
275 280 285
Ile Pro Ile Ala Tyr Thr Leu Val Thr Ser Arg Phe Leu Ile Leu Trp
290 295 300
His Ser Ala Leu Pro Leu Ala Leu Trp Asn Asp Cys Gly Trp Leu Thr
305 310 315 320
Ile Pro Ala Thr Phe Leu Thr Gly Met Ala Leu Phe Tyr Ile Glu Glu
325 330 335
Val Gly Val Val Ile Glu Glu Pro Phe Trp Ile Leu Pro Leu Gly Ser
340 345 350
Ile Cys Thr Gly Ile Val Ser Ala Ile Asp Gly Leu Ser Ala Ala His
355 360 365
Lys Thr Ser Asn Leu Leu Trp Ser Leu His Gln Pro Met Thr Asp His
370 375 380
Val Val Gln Ile Ala Phe Glu Arg Ser Lys Thr Asp Gly Phe Ser Lys
385 390 395 400
Thr Arg Asp Gln Ile Asp Val Met Leu Thr Arg Ile Asn Ser Ile Ala
405 410 415
<210> 54
<211> 297
<212> PRT
<213> 小立碗藓
<400> 54
Met Gly Leu His Lys Ser Ser Trp Arg His Gly Arg His Ile Gln Thr
1 5 10 15
Ala Phe Ser Thr Gly Val Ile Ser Ser Ile Ile Pro Arg Val Phe Phe
20 25 30
Cys Thr Leu Ile Ser Val Leu Val Thr Ile Phe Asn His Ala Val Met
35 40 45
Glu Gly Val Leu Pro His Trp Val Pro Ser Leu Arg Val Pro Thr Leu
50 55 60
Pro Met Ser Leu Thr Ala Pro Val Leu Ser Leu Leu Leu Val Phe Arg
65 70 75 80
Thr Asn Ser Ser Tyr Asn Arg Leu Asp Glu Ala Arg Lys Ala Trp Gly
85 90 95
Ser Asn Val Asn Arg Thr Arg Asp Val Ser Arg Gln Ala Leu Ser Trp
100 105 110
Ile Cys Asp Pro Asp Asp Ala Asp Lys Leu Gln Ser Leu Leu Arg His
115 120 125
Ile Lys Ala Phe Ser Tyr Cys Leu Lys Asp His Leu Thr Gln Glu Asn
130 135 140
Leu Leu Gln Glu Glu Leu Ala Arg Val Leu Glu Pro Arg Glu Val Glu
145 150 155 160
Leu Val Leu Lys Ser Ser His Arg Pro Asn Tyr Val Leu His Val Met
165 170 175
Ser Asp Thr Ile Lys His Cys Arg Ile Ser Lys Trp Glu Ser Lys Ser
180 185 190
Met Asp Arg Asn Ile Thr Gln Phe His Asp Asn Val Gly Ala Cys Glu
195 200 205
Arg Leu Phe Lys Thr Pro Ile Pro Val Ala Tyr Thr Arg Met Ile Ser
210 215 220
Arg Phe Leu Ser Ile Trp His Phe Leu Leu Pro Leu Ala Leu Trp Asn
225 230 235 240
Ser Cys Arg Trp Leu Thr Ile Pro Val Thr Phe Val Ser Gly Val Gly
245 250 255
Leu Phe Cys Ile Glu Glu Val Gly Val Leu Ile Glu Asp Pro Ser Thr
260 265 270
Ser Leu Leu Cys Cys Gln Ser Ala Met Ala Phe Pro Gln Leu Trp Met
275 280 285
Ala Ser Ala Arg Leu Met Lys Glu Leu
290 295
<210> 55
<211> 669
<212> PRT
<213> 近头状尖胞藻
<400> 55
Met Leu Ala Thr Ala Pro Cys Ala Ala Ala Cys Thr Pro Ser Arg Arg
1 5 10 15
Gly Gly Arg Cys His Arg Arg Leu Trp Thr Pro Pro Arg Thr Ala Ala
20 25 30
His Gly Gln His Ala Ala Ala Asp Gly Asp Lys Gln Gln Gln Pro Asp
35 40 45
Gln Pro Pro Pro Pro Pro Ala Thr Ala Ala Ala Ala Gly Ser Asp Pro
50 55 60
Ala Pro Pro Ser Pro Ala Ala Ala Pro Pro Pro Thr Pro Pro Gly Gly
65 70 75 80
Phe Thr Pro Tyr Trp Pro Ser Asn Tyr Arg Lys Arg Leu Glu Ala Ala
85 90 95
Thr Leu Glu Arg Ala His Glu Ala Asp Val Thr Pro Thr Val Phe Asp
100 105 110
Phe Ala Leu Trp Arg Arg His Arg Ser Arg Leu Arg Tyr Val Glu His
115 120 125
Leu Val Thr Leu Thr Gly Ser Tyr Phe Val Leu Asp Leu Ala Ala Pro
130 135 140
Val Ile Val Leu Thr Ala Ala Ala Ala Ala Val Gly Leu Tyr Glu Thr
145 150 155 160
Val Leu Gln Gly Gly Met Leu Pro Ser Phe Leu Pro Asn Ile Ala Met
165 170 175
Ala Ala Glu Gly Pro Phe Asn Ala Ala Ser Phe Ala Met Ser Leu Met
180 185 190
Leu Ala Phe Lys Thr Asn Val Ser Tyr Ala Arg Trp Asn Glu Ala Arg
195 200 205
Cys Leu Trp Gly Ser Leu Val Asn Arg Ser Arg Asn Leu Val Arg Gln
210 215 220
Gly Leu Met Leu Ile Pro Arg Glu His Ala Ala Leu Lys Pro Val Leu
225 230 235 240
Ala Arg Trp Ser Ile Ala Phe Ala Arg Ala Leu Lys Thr His Leu Arg
245 250 255
Glu Asp Gly Asp Val Ala Gly Glu Leu Ala Ala Ile Leu Glu Pro His
260 265 270
Glu Val Ala Val Val Ala Ala Asn Leu Asn Arg Pro Leu Ala Cys Leu
275 280 285
Gln Ala Met Ser Ala Val Val Arg Ala Ala Arg Leu Asp Pro Ile Gly
290 295 300
Thr Ser Arg Leu Asp Ile Asn Leu Glu Thr Phe Glu Asp Ile Met Gly
305 310 315 320
Ala Cys Glu Arg Ile Leu Lys Thr Pro Ile Pro Leu Ser Tyr Thr Arg
325 330 335
His Thr Ser Arg Phe Leu Thr Leu Trp Leu Ala Thr Leu Pro Phe Val
340 345 350
Leu Trp Asn His Ile His Trp Gly Val Ile Pro Ser Thr Ala Val Ile
355 360 365
Ser Phe Leu Leu Phe Gly Ile Asp Glu Ile Ala Ile Gln Leu Glu Glu
370 375 380
Pro Phe Gly Ile Leu Pro Leu Glu Ala Ile Cys Asp Thr Ile Gln Arg
385 390 395 400
Asn Ile Glu Glu Leu Val Asn Ser Glu Gly Ala Val Ala Leu Ala Leu
405 410 415
Asp Ala Ser Met His Glu Ala Arg Ala Arg Leu Ala Gln Glu Glu Glu
420 425 430
Leu Ala Arg Met Leu Glu Glu Gln Leu Pro Phe Asn Trp Gly Pro Arg
435 440 445
Gly Leu Ser Gln Pro His Ser Pro Ala Ala Ala Pro Gly Thr Pro Gly
450 455 460
Ala Ala Gly Gly Ala Gly Ala Ala Ala Ala Ala Ala Ala Leu Ile Ala
465 470 475 480
Ala Asn Arg Pro Arg Pro Phe Gln Asp Phe Pro Ala Gly Gln Pro Ala
485 490 495
Thr Arg Ile Glu Pro Trp Arg Glu Glu Pro Val Gly Ala Thr Ala Pro
500 505 510
Pro Pro Val Ser Glu Glu Gly Gln Pro Pro Gln Trp Arg Pro Gly Trp
515 520 525
Gly Arg Gly Gly Asp Gly Phe Gly Gln Gln Pro Glu Cys Pro Pro Asn
530 535 540
Cys Ser Cys Arg Met Ala Glu Pro Gly Ala Ala Gly Gly Gln Gln His
545 550 555 560
Ser Ser Pro Ala Leu Arg Arg Glu Asp Ala Asp Arg Leu Arg Arg Ala
565 570 575
Leu Gly Glu Val Ala Ala Arg Arg Ala Ala Gln Ser Glu Ala Arg Ala
580 585 590
Ala Pro Leu Glu Pro Val Glu Met Pro Trp Tyr Ala Asp Asp Val Ala
595 600 605
Ser Asp Ser Leu Leu Gly Ala Ala Ala Ala Glu Ala Asp Pro Arg Glu
610 615 620
Ala Gly Val Val Glu Phe Leu Leu Gly Arg Ala His Gly Ala Asn Ala
625 630 635 640
Val Arg His Pro Gly Thr Pro Met Gly Thr His Glu Glu Glu Glu Glu
645 650 655
Gly Pro Ala Ala Ala Gly Pro Ala Pro Pro Ala Gly Gly
660 665
<210> 56
<211> 564
<212> PRT
<213> 近头状尖胞藻
<400> 56
Met Arg Ser Ser Leu Ala Ala Pro Pro Ala His Ala Ser Ser Pro Arg
1 5 10 15
Gly Leu Arg Ala Ala Ala Pro Pro Arg Pro Arg Ala Gly Ala Arg Gly
20 25 30
Gly Arg Pro Ala Val Val Val Ala Ala Leu Ser Gln Glu Asp Asp Gln
35 40 45
Ile Lys Glu Gln Ser Arg Lys Phe Arg Arg Thr Val Phe Thr Phe Glu
50 55 60
Asp Trp Glu Lys His Arg Ser Thr Ser Arg Tyr Ser Arg His Leu Ser
65 70 75 80
Gly Met Phe Ser Ser Arg Val Val Trp Gly Leu Gln Arg Pro Leu Val
85 90 95
Tyr Val Cys Gly Val Ser Leu Ala Val Ile Leu Tyr Glu Lys Ser Leu
100 105 110
Glu Leu Leu Ser Arg Glu Pro Phe Gly Leu Thr Ser Phe Ala Leu Ser
115 120 125
Leu Leu Leu Val Phe Arg Thr Asn Val Ser Tyr Glu Arg Trp Asp Ser
130 135 140
Ala Arg Lys Met Trp Gly Leu Leu Leu Asn Arg Ser Arg Asp Leu Val
145 150 155 160
Arg Gln Gly Ile Thr Tyr Ile Pro Glu Asp Arg Pro Glu Leu Arg Thr
165 170 175
Leu Leu Val Arg Trp Thr Pro Ala Phe Ala Arg Ala Leu Leu Cys His
180 185 190
Leu Arg Lys Asp Gly Asp Trp Glu Gly Gln Leu Gly Leu Thr Leu Lys
195 200 205
Pro His Glu Val Glu Gln Val Leu Lys Ala Thr His Arg Pro Asn Tyr
210 215 220
Val Leu Gln Val Leu Ser Gln Ile Ile Arg Glu Ala Asp Leu Gly Val
225 230 235 240
Ala Pro Thr Thr Arg Met Asp Glu Asn Leu Thr Gln Phe Glu Asp Val
245 250 255
Leu Gly Gly Cys Glu Arg Ile Leu Lys Thr Pro Val Pro Leu Ser Tyr
260 265 270
Thr Arg His Thr Ser Arg Phe Leu Met Ile Trp Leu Thr Leu Leu Pro
275 280 285
Phe Thr Leu Tyr Ser Thr Val Gly Leu Gly Thr Ala Pro Leu Cys Ala
290 295 300
Ile Ile Ala Phe Leu Leu Leu Gly Ile Glu Glu Ile Gly Val Ser Ile
305 310 315 320
Glu Glu Pro Phe Ser Ile Leu Ala Leu Glu Ala Ile Ser Asp Ser Ser
325 330 335
Leu Asn Asn Ser Arg Glu Leu Ala Ala Met His Ala Thr Asp Ala Ala
340 345 350
Val Pro Leu Pro Glu Gly Ala Asp Pro Pro Pro Ala Tyr Thr Ser Ala
355 360 365
Arg Asp Leu Ile Ala Leu Ala Ser Ala Ala Ala Val Ala Ser Ala Ala
370 375 380
Ala Asp Ala Leu Ala Ala Pro Ala His Ala Pro Ala Pro Ala Ser His
385 390 395 400
Ser Ala Pro Ala Pro Gln Glu Pro Ala Ala Pro Ala Ala Pro Ala Ala
405 410 415
Val Ala Pro Ala Ala Ala Ala Pro Ala Pro Ala Ala Pro Ala Pro Thr
420 425 430
Ala Pro Ala His Ser Ala Pro Val Pro Lys Glu Pro Ala Ala Glu Pro
435 440 445
Glu Arg Val Pro Val Leu Ala Gly Val Gly Ala Gly Arg Ala Pro Val
450 455 460
His Leu Asn Gly Lys Ser Gly Asn Gly Ala Thr Ala Tyr Glu Pro Ala
465 470 475 480
Ala Ala Pro Ala Arg Ala Asn Gly Ala Arg Pro Ala Ala Ala Ala Ser
485 490 495
Pro Lys Pro Ala Ala Ala Ala Ala Pro Ala Ala Ala Ser His Ala Ala
500 505 510
Pro Ala Ala Pro Ala Ala Ala Ala Ala Pro Gln Ala Ala Ala Ala Pro
515 520 525
Gln Ala Ala Ala Pro Ala Pro Ala Pro Ala Ala Lys Pro Leu Pro Asn
530 535 540
Thr Gly Arg Ala Gly Ser Leu Arg Arg Thr Gly Gly Tyr Gly Leu Ile
545 550 555 560
Arg Phe Arg Ser
<210> 57
<211> 513
<212> PRT
<213> 近头状尖胞藻
<400> 57
Met Gln Cys Ala Ile Gly Asn Ala Ala Val Gln Pro Leu Gly Arg Pro
1 5 10 15
Leu Gly Val Pro Arg Ser Arg Ala Ala Ala Ala Ala Ala Ala Ala Val
20 25 30
Arg Ala Arg Ser Trp Pro Arg Leu Thr Ala Leu Arg Ala Ala Pro Gly
35 40 45
Asp Gly Pro Glu Pro Ser Ala Pro Ser Thr Ala Gly Ser Leu Gln Ser
50 55 60
Leu Ala Glu Arg Leu Arg Ala Thr Ala Thr Pro Gly Glu Val Gln Ser
65 70 75 80
Asp Leu Asn Tyr Tyr Gly Leu Ala Asn Leu Glu Asp Ala Arg Arg Ser
85 90 95
Gly Asp Glu Ala Gln Asp Asp Leu Leu Trp Arg Thr Pro Val Ser Glu
100 105 110
Leu Ala Ala Arg Arg Arg Gly Leu Gly Leu Val Ala Ala Asp Leu Ser
115 120 125
Thr Glu Gln Glu Arg Lys Leu Arg Arg Ala Val Phe Gly Phe Asp Arg
130 135 140
Trp Ala Ala His Arg Ser Thr Ser Arg Tyr Leu Arg His Leu Lys Gly
145 150 155 160
Ile Val Ser Ser Arg Thr Met Arg Ala Leu Leu Pro Pro Leu Ala Phe
165 170 175
Phe Gly Ala Val Ala Val Ala Ala Gly Trp Tyr Thr Leu Glu Leu Ala
180 185 190
Pro Gln Tyr Gly Leu Pro Lys Val Val Leu Asn Asp Ser Ser Leu Glu
195 200 205
Ile Thr Ser Phe Ala Leu Ser Leu Leu Leu Val Phe Arg Thr Asp Ser
210 215 220
Ser Tyr Ala Arg Trp Glu Gln Ala Leu Asn Ala Trp Gln Ser Val Arg
225 230 235 240
Ala Glu Ser Lys Asn Leu Ala Arg Gln Ala Cys Tyr Phe Val Glu Glu
245 250 255
Pro Ile Arg Arg Ala Met Leu Met Arg Trp Thr Val Ala Phe Pro His
260 265 270
Ala Leu Leu Ser His Val Arg Cys Asp Ala Pro Leu Arg Ala Gln Leu
275 280 285
Ala Gly Val Leu Ala Pro His Glu Val Glu Ala Val Glu Ala Val Gly
290 295 300
Ala Ala Ser Ala Pro Glu Phe Val Leu Gln Val Met Ser Glu Leu Val
305 310 315 320
Glu Gln Cys Arg Leu Gly Asp Ser His Arg Ala Glu Gln Leu Tyr Phe
325 330 335
Ser Ile Lys Ala Leu Gly Gly Ala Val Gly Thr Cys Asp Lys Leu Leu
340 345 350
Arg Tyr Pro Ile Pro Leu Ala Tyr Ser Arg His Thr Ser Arg Phe Met
355 360 365
Cys Val Trp Leu Ser Ala Leu Pro Phe Ala Leu Phe Ser Ser Gly Leu
370 375 380
Gly Trp Gly Ala Val Pro Val Thr Leu Val Thr Ala Tyr Leu Leu Leu
385 390 395 400
Ala Val Asp Glu Ile Gly Val Gln Ile Glu Glu Pro Phe Ser Ile Leu
405 410 415
Pro Leu Glu Asp Ile Leu Phe Asp Ile Gln Ser Glu Thr Ala Ala Leu
420 425 430
Gly Glu Arg Gln Gly Ala Val Ala Glu Ile Leu Arg Ala Gly Gly Ile
435 440 445
Glu Cys Thr Pro Arg Thr Pro Phe Asp Asp Ala Ala Cys Ala Ala Cys
450 455 460
Gly Ala Leu Val Ser Pro Ala Gly Ser Leu Asp Asp Gly Asp Asp Ala
465 470 475 480
Gly Gly Gly Gly Ala Gly Ala Gly Ala Gly Glu Ala Leu Pro Gly Leu
485 490 495
Pro Leu Arg Pro Arg Ala Pro Gly Gly Arg Arg Val Val Arg Thr Ser
500 505 510
Arg
<210> 58
<211> 469
<212> PRT
<213> 近头状尖胞藻
<400> 58
Met Lys Leu Ser Ala Gly Pro Met Gln Ser Arg Ala Ala Phe Ala Gly
1 5 10 15
Gln Arg Leu Ala Val Arg Pro Ala Leu Arg Ser Arg Ala Ala Lys Arg
20 25 30
Thr Pro Ala Val Gln Thr Ser Cys Gly Ala Ala Pro Lys Gly Leu Pro
35 40 45
Ser Ser Val Thr Ser Tyr Asp Asp Asp Ile Lys Glu Lys Ser Arg Lys
50 55 60
Phe Arg Arg Thr Val Phe Thr Phe Glu Ser Trp Glu Gly His Arg Ser
65 70 75 80
Thr Glu Arg Tyr Ala Arg His Ile Ile Ser Met Phe Ser Ser Arg Val
85 90 95
Val Arg Gly Leu Ser Lys Pro Leu Phe Tyr Met Val Gly Val Ser Ser
100 105 110
Phe Val Val Ala Tyr Glu Thr Ala Arg Leu Ala Gly Leu Leu Pro Leu
115 120 125
Trp Ala Pro Ser Val Gln Leu Asn Ser Lys Glu Val Phe Gly Leu Thr
130 135 140
Ser Phe Ala Leu Ser Leu Leu Leu Val Phe Arg Thr Asn Ala Ser Tyr
145 150 155 160
Glu Arg Trp Asp Gly Ala Arg Lys Met Trp Gly Leu Val Leu Asn Arg
165 170 175
Ser Arg Asp Ile Val Arg Gln Gly Leu Thr Tyr Phe Pro Ser Asp Arg
180 185 190
Pro Glu Leu Lys Asp Met Leu Val Arg Trp Thr Pro Ala Phe Ser Lys
195 200 205
Ser Leu Met Cys His Leu Arg Lys Asp Asp Asp Tyr Arg Glu Val Leu
210 215 220
Gln Asp Ile Leu Leu Pro His Glu Leu Asp Ser Ile Met Met Ala Thr
225 230 235 240
His Arg Pro Asn Tyr Ala Leu Gln Val Leu Ser Glu Ile Val Arg Glu
245 250 255
Ala Asn Cys Gly Thr Ala Pro Thr Met Arg Met Asp Asp Asn Ile Thr
260 265 270
Ser Phe Glu Asp Cys Leu Gly Gly Cys Glu Arg Ile Leu Lys Thr Pro
275 280 285
Val Pro Leu Ser Tyr Thr Arg His Thr Ser Arg Phe Leu Met Ile Trp
290 295 300
Leu Ser Leu Leu Pro Phe Ala Leu Tyr Gly Thr Cys Gly Val Ser Thr
305 310 315 320
Ile Pro Leu Cys Val Ile Ile Ala Phe Leu Leu Leu Gly Ile Glu Glu
325 330 335
Ile Gly Val Ser Ile Glu Glu Pro Phe Ser Ile Leu Ala Leu Glu Ala
340 345 350
Ile Ser Asp Ser Ala Leu Asn Asn Ser Arg Glu Leu Lys Ala Gln His
355 360 365
Asp Ala Glu Val Arg Asn Gly Asn Gln Met Ser Ala Thr Asp Leu Ile
370 375 380
Ala Phe Glu Ala Ala Ala Arg Gly Arg Thr Pro Leu Ser Lys Leu Ser
385 390 395 400
Gly Ala Pro Ala Ala Ala Pro Ala Pro Val Pro Ala Ala Ala Pro Ala
405 410 415
Pro Ala Ala Ala Pro Ala Pro Ala Pro Ala Pro Val Pro Ala Ala Ala
420 425 430
Pro Ala Tyr Val Ala Ala Ala Ala Thr Asn Gly Ser Ala Asn Gly Ala
435 440 445
Ala Pro Asn Thr Trp Pro Arg Pro Arg Ser Pro Val Pro Thr Ser Gly
450 455 460
Val Ser Phe Arg Lys
465
<210> 59
<211> 443
<212> PRT
<213> 近头状尖胞藻
<400> 59
Met Ala Leu Ala Ala Ala Arg Pro Ala Leu Cys Ala Gly Pro Ser Gln
1 5 10 15
Leu Arg Arg Ala Gly Gln Pro Ala Ala Gln Ala Ala Arg Pro Leu Leu
20 25 30
Pro Leu Arg Arg Ala Arg Ala Pro Leu Arg Val Gln Ala Ser Lys Ala
35 40 45
Asn Pro Tyr Ala His Met Asn Val Asp Asp Ala Ile Lys Glu Gln Thr
50 55 60
Arg Lys Thr Ala Arg Thr Val Phe Asp Phe Glu Arg Trp Glu Ala His
65 70 75 80
Arg Cys Ser Gly Arg Tyr Phe Arg His Ile Leu Gly Val Phe Asn Ser
85 90 95
Arg Ile Ile Phe Gly Leu Leu Arg Pro Leu Met Leu Val Ile Leu Thr
100 105 110
Ala Thr Ala Val Val Met Trp Glu Ser Leu Arg Gln Ala Gly Val Leu
115 120 125
Pro Pro Val Val Pro Ser Val Gln Leu Ala Trp Ser Lys Glu Leu Phe
130 135 140
Gly Leu Thr Ser Phe Ala Leu Ser Leu Leu Leu Val Phe His Thr Asn
145 150 155 160
Ala Gly Tyr Glu Arg Trp Asp Gly Ala Arg Lys Ile Trp Gly Leu Met
165 170 175
Leu Asn Arg Ser Arg Asp Ile Ala Arg Gln Gly Leu Thr Tyr Ile Pro
180 185 190
Arg Asp Gln Pro His Leu Arg Ala Met Leu Cys Arg Trp Val Pro Ala
195 200 205
Phe Ser Arg Thr Leu Met Cys His Leu Arg Gln Asp Ser Asp Phe Gly
210 215 220
Ala His Leu Lys Gly Val Leu Tyr Pro His Glu Ile Glu Ala Val Leu
225 230 235 240
Ser Ser Thr His Arg Pro Asn Tyr Val Leu Gln Val Ile Ser Glu Ile
245 250 255
Val Lys Ser Ala Arg Leu Pro Leu Ala Ala Arg Val Lys Met Asp Gln
260 265 270
Asn Ile Thr Thr Phe His Asp Asp Leu Gly Gly Cys Glu Arg Ile Leu
275 280 285
Lys Thr Pro Ile Pro Leu Ser Tyr Thr Arg His Thr Gly Arg Phe Leu
290 295 300
Met Ile Trp Leu Leu Leu Leu Pro Phe Thr Leu Val Gly Gly Cys Gly
305 310 315 320
Val Thr Thr Ile Pro Leu Cys Ala Thr Ile Gly Phe Leu Leu Met Gly
325 330 335
Ile Asp Glu Ile Gly Val Ala Ile Glu Glu Pro Phe Ser Ile Leu Pro
340 345 350
Leu Glu Ala Ile Ala Asn Ser Ala Leu Val Asn Ile Arg Glu Leu Glu
355 360 365
Ala Ala His Gln Ala Asp Pro Glu Pro Asp Phe Ser Ala Ala Glu Asp
370 375 380
His His Thr Arg Thr Ala Leu Asp Leu Val Ser Ala Thr Ala Thr Ala
385 390 395 400
Gly Thr Ala Gln Arg Ala Ala Ala Ala Ala Ala Thr Ala Ala Ser Ala
405 410 415
Ala Ala Val Ala Ala Ala Ala Ala Ala Ala Ala Ala Ala Gly Ala Pro
420 425 430
Arg Ser Trp Pro Ser Ser Pro Ala Phe Ala Asn
435 440
<210> 60
<211> 1146
<212> PRT
<213> 四豆藻
<400> 60
Met Gln Ala Leu Arg Leu Lys Gly Gln Arg Pro Gly Leu Arg Ala Ser
1 5 10 15
Leu Gln Pro Arg Arg Thr Val Val Arg Ile Val Ala Asn Ala Ala Lys
20 25 30
Asp Pro Asn Ala Pro Ile Gln Ser Asn Pro Leu Gly Thr Leu Ser Ser
35 40 45
Gln Ser Gly Thr Val Ala Pro Leu Leu Arg Ser Glu Glu Ala Arg Arg
50 55 60
Tyr Trp Arg Thr Val Tyr Asp Phe Pro Gln Trp Gln Lys His Arg Ser
65 70 75 80
Ser Tyr Arg Phe Met Glu Arg Leu Phe Gln Leu Leu Gln Ser His Ile
85 90 95
Leu Gln Asn Ala Leu Pro Ser Ile Ser Trp Val Thr Leu Val Ala Thr
100 105 110
Leu Val Ala Ala Tyr Gly Thr Ser Phe Asp Gln Gly Leu Leu Pro Asp
115 120 125
Gly Leu Pro Asn Ile Ser Pro Asn Ala Ser Cys Thr Ala Phe Ile Ser
130 135 140
Asn Thr Ser Ile Ala Leu Ser Leu Leu Leu Val Phe Arg Thr Asn Ser
145 150 155 160
Ser Tyr Gly Arg Trp Asp Glu Ala Arg Lys Met Trp Gly Gly Leu Leu
165 170 175
Asn Arg Ser Arg Asp Ile Met Arg Gln Gly Ala Thr Cys Phe Pro Asp
180 185 190
Asp Gln Val Glu Ala Lys Lys Ala Leu Ala Arg Trp Val Val Ala Tyr
195 200 205
Ala Arg Ala Leu Arg Ile His Phe Gln Pro Glu Val Thr Leu Glu Ser
210 215 220
Glu Leu Lys Asn Ile Leu Thr Ala Pro Glu Leu Glu Met Leu Ala Arg
225 230 235 240
Ser Gln His Arg Pro Val Arg Ala Ile His Ser Ile Ser Gln Ile Ile
245 250 255
Gln Ser Val Pro Met Ser Ser Ile His Gln Met Gln Met Ser Asn Asn
260 265 270
Leu Thr Phe Phe His Asp Val Leu Gly Gly Cys Glu Arg Leu Leu Arg
275 280 285
Ala Pro Ile Pro Val Ser Tyr Thr Arg His Thr Ala Arg Phe Leu Phe
290 295 300
Ala Trp Leu Thr Leu Leu Pro Phe Ala Leu Tyr Pro Thr Val Gly Trp
305 310 315 320
Gly Val Val Pro Val Ser Thr Gly Ile Ala Ala Val Leu Cys Gly Ile
325 330 335
Glu Glu Ile Gly Val Gln Cys Glu Glu Pro Phe Gly Ile Leu Pro Leu
340 345 350
Asp Val Ile Cys Asn Arg Ile Gln Ala Asp Val Met Ala Thr Leu Lys
355 360 365
Asp Asp Gln Asp Thr Lys Ile Val Leu Ser Glu Ala Gly Leu Leu Gly
370 375 380
Leu Leu Pro Ser Ala Val Ala Thr Val Pro Val Ala Ala Ala Ala Ala
385 390 395 400
Ala Ala Ala Ala Ala Ala Ala Ala Pro Ala Asn Gly Asn Gly Asn Gly
405 410 415
Asn Gly Asn Gly Asn Gly Ala Tyr Ala Gln Pro Ala Gly Ala Val Ala
420 425 430
Ser Lys Asp Met Gln Val Arg Val Ala Ile Gly Ala Glu Lys His Gln
435 440 445
Arg Val Cys Ala Pro Arg Gly Leu Arg Ala Pro Ala Leu Cys Gly Arg
450 455 460
Arg Leu Arg Leu Val Val Ser Ala Asp Ala Ala Lys Asp Pro Asn Ala
465 470 475 480
Pro Ile Gln Ser Asn Pro Leu Gly Thr Leu Ser Ser Gln Ser Gly Leu
485 490 495
Val Ser Thr Thr Pro Arg Ser Glu Met Ala Arg Lys Tyr Phe Arg Thr
500 505 510
Val Tyr Asp Phe Pro Gln Trp Gln Lys His Arg Asn Gln Tyr Arg Leu
515 520 525
Met Lys Arg Leu Ile Thr Ile Pro Gln Ser His Ile Ile Gln Asn Ala
530 535 540
Leu Pro Ser Ile Ala Trp Val Gly Leu Val Ser Thr Ala Leu Ala Val
545 550 555 560
Tyr Met Thr Ala Leu Asp Gln His Leu Leu Pro Glu Gly Leu Pro Ser
565 570 575
Phe Ala Pro Asn Ala Ser Cys Ser Ala Phe Ile Ser Asn Thr Ser Val
580 585 590
Ala Leu Ser Leu Leu Leu Val Phe Arg Thr Asn Thr Ser Tyr Ser Arg
595 600 605
Trp Asp Glu Ala Arg Lys Met Trp Gly Gly Leu Leu Asn Arg Ser Arg
610 615 620
Asp Ile Met Arg Gln Ala Ala Thr Cys Phe Pro Asp Asp Gln Val Glu
625 630 635 640
Ala Lys Lys Ala Leu Ala Arg Trp Val Val Ala Tyr Ala Arg Ala Leu
645 650 655
Arg Ile His Phe Gln Pro Glu Val Thr Leu Glu Ser Glu Leu Lys Asn
660 665 670
Ile Leu Thr Pro Ala Glu Leu Glu Met Leu Ala Arg Ser Gln His Arg
675 680 685
Pro Val Arg Ala Ile His Ser Ile Ser Gln Ile Ile Gln Ser Val Pro
690 695 700
Met Ser Ser Val His Gln Met Gln Met Ser Asn Asn Leu Thr Phe Phe
705 710 715 720
His Ala Lys Asp Pro Asn Ala Pro Ile Gln Ser Asn Pro Leu Gly Thr
725 730 735
Leu Ser Ser Gln Ser Gly Leu Val Ser Thr Thr Pro Arg Ser Glu Met
740 745 750
Ala Arg Lys Tyr Phe Arg Thr Val Tyr Asp Phe Pro Gln Trp Gln Lys
755 760 765
His Arg Asn Gln Tyr Arg Leu Met Lys Arg Leu Ile Thr Ile Pro Gln
770 775 780
Ser His Ile Ile Gln Asn Ala Leu Pro Ser Ile Ala Trp Val Gly Leu
785 790 795 800
Val Ser Thr Ala Leu Ala Val Tyr Met Thr Ala Leu Asp Gln His Leu
805 810 815
Leu Pro Glu Gly Leu Pro Ser Phe Ala Pro Asn Ala Ser Cys Ser Ala
820 825 830
Phe Ile Ser Asn Thr Ser Val Ala Leu Ser Leu Leu Leu Val Phe Arg
835 840 845
Thr Asn Thr Ser Tyr Ser Arg Trp Asp Glu Ala Arg Lys Met Trp Gly
850 855 860
Gly Leu Leu Asn Arg Ser Arg Asp Ile Met Arg Gln Ala Ala Thr Cys
865 870 875 880
Phe Pro Asp Asp Gln Val Glu Ala Lys Lys Ala Leu Ala Arg Trp Val
885 890 895
Val Ala Tyr Ala Arg Ala Leu Arg Ile His Phe Gln Pro Glu Val Thr
900 905 910
Leu Glu Ser Glu Leu Lys Asn Ile Leu Thr Pro Ala Glu Leu Glu Met
915 920 925
Leu Ala Arg Ser Gln His Arg Pro Val Arg Ala Ile His Ser Ile Ser
930 935 940
Gln Ile Ile Gln Ser Val Pro Met Ser Ser Val His Gln Met Gln Met
945 950 955 960
Ser Asn Asn Leu Thr Phe Phe His Asp Val Leu Gly Gly Cys Glu Arg
965 970 975
Leu Leu Arg Ala Pro Ile Pro Val Ser Tyr Thr Arg His Thr Ala Arg
980 985 990
Phe Leu Phe Ala Trp Leu Thr Leu Leu Pro Phe Ala Leu Tyr Gly Gln
995 1000 1005
Leu Gly Tyr Ala Val Val Pro Thr Cys Leu Gly Ile Ala Ala Val Leu
1010 1015 1020
Cys Gly Ile Glu Glu Ile Gly Val Gln Cys Glu Glu Pro Phe Gly Ile
1025 1030 1035 1040
Leu Pro Leu Asp Val Ile Cys Asn Arg Ile Gln Ala Asp Val Met Ala
1045 1050 1055
Thr Leu Lys Asp Asp Glu Asp Thr Lys Ile Val Leu Ala Glu Ala Gly
1060 1065 1070
Leu Leu Asn Leu Leu Pro Arg Pro Ala Ala Leu Ala Leu Glu Pro Ala
1075 1080 1085
Val Gln Tyr Ala Ala Ala Ser Asn Gly Asn Gly Asn Gly Ala Ser Thr
1090 1095 1100
Asn Gly Asn Gly Ala Ser Thr Asn Gly Asn Gly Ala Ser Thr Asn Gly
1105 1110 1115 1120
Asn Gly Asn Gly Lys Pro Ala Leu Val Thr Val Gly Ala Ser Ser Ala
1125 1130 1135
Ala Ala Met Lys Val Glu Ile Thr Pro Arg
1140 1145
<210> 61
<211> 705
<212> PRT
<213> Volvox carteri f. nagariensis
<400> 61
Met Gln Ser Gln Leu Gln Pro Arg Leu Gln Leu Gln Gly Thr Arg Leu
1 5 10 15
Asn Trp Leu Pro Gln Arg Ser Cys Val Gln Arg Arg Ser Leu Arg Val
20 25 30
Asp Ala Thr Ser Gly Ala Ala Pro Pro Pro Pro Ala Gly Lys Glu Leu
35 40 45
Ser Asn Asp Met Val Thr Arg Gln Tyr Arg Arg Thr Val Tyr Asp Phe
50 55 60
Ser Leu Trp Ala Lys His Arg Asp Val Asn Arg Tyr Leu Tyr Asn Leu
65 70 75 80
Lys Thr Ile Pro Gly Ser Arg Ile Ile Arg Thr Leu Gly Gln Pro Met
85 90 95
Gly Ile Val Leu Ala Trp Ala Ala Met Phe Gly Phe Tyr Glu Thr Cys
100 105 110
Leu Glu Ser Gly Val Leu Pro Ser Tyr Phe Pro Lys Leu Thr Leu Met
115 120 125
Ser Ala Glu Pro Gln Gly Leu Thr Ser Phe Ala Leu Ser Leu Leu Leu
130 135 140
Val Phe Arg Thr Asn Ser Ser Tyr Gly Arg Phe Asp Glu Ala Arg Lys
145 150 155 160
Ile Trp Gly Gly Ile Leu Asn Arg Ala Arg Asn Ile Ala Asn Gln Ala
165 170 175
Val Thr Phe Ile Pro Ala Glu Asp Val Ala Gly Arg Glu Ala Val Gly
180 185 190
Lys Trp Ala Val Gly Phe Cys Arg Ala Leu Gln Ala His Leu Gln Glu
195 200 205
Asp Ala Asn Leu Arg Glu Glu Leu Gln Lys Ala Gln Pro Arg Trp Ser
210 215 220
Arg Glu Glu Ile Asp Met Leu Cys Ser Ala Gln His Ser Trp Gln Gln
225 230 235 240
Leu Gln Ser Cys Val Asn Ala Phe Trp Pro Ile Lys Ala Ile Ser Met
245 250 255
Leu Ser Glu Leu Thr Arg Gln Leu Pro Ile Ser Gln Phe Gln Ala Leu
260 265 270
Gln Met Gln Glu Asn Val Thr Phe Phe Tyr Asp Ala Leu Gly Gly Cys
275 280 285
Glu Arg Leu Leu Arg Thr Pro Ile Pro Val Ser Tyr Thr Arg Ile Leu
290 295 300
Pro Leu Asp Ala Ile Cys Thr Arg Ala Gln Thr Asp Val Val Ser Leu
305 310 315 320
Leu Lys Asp Asp Pro Ala Val Val Lys Tyr Ile Ser Asp Val Arg Gln
325 330 335
Gly Arg Ile Ala Pro Pro Thr Glu Pro Pro Val Ala Gly Ala Ala Pro
340 345 350
Val Ala Ala Ala Pro Pro Pro Pro Pro Pro Ala Ser Ala Gly Gly Gly
355 360 365
Ile Ser Arg Ser Gly Ser Pro Thr Ala Gln Gln Gln Pro Asp Val Met
370 375 380
Lys Thr Val Thr Ser Met Leu His Asn Val Lys Ala Gly Ile Gly Ala
385 390 395 400
Val Ala Pro Ala Pro Pro Arg Pro Pro Ser Pro Gln Pro Arg Ala Arg
405 410 415
Ser Pro Arg Ala Ala Ser Pro Gly Gly Pro Ser Pro Phe Pro Arg Ala
420 425 430
Ser Ala Gly Thr Gly Gly Ala Ala Ala Ala Val Pro Ser Pro Pro Pro
435 440 445
Ile Lys Pro Leu Thr Ser Ser Ser Ser Ser Ser Ser Gly Ala Val Ser
450 455 460
Lys Asp Ser Asn Asn Ser Thr Ala Thr Ala Lys Lys Pro Ala Ser Ala
465 470 475 480
Pro Ala Ala Ser Ser Ala Gly Phe Ser Met Gly Phe Ser Gly Leu Ala
485 490 495
Asp Gly Ala Ala Ala Ala Ala Lys Ser Ala Ser Ala Ala Ala Ala Lys
500 505 510
Phe Ser Lys Ile Ala Asp Ser Val Val Ala Gly Thr Pro Ala Ala Pro
515 520 525
Ala Ser Glu Ala Lys Arg Glu Thr Ala Ala Ala Ala Ala Met Gln Ala
530 535 540
Gln Pro Arg Asn Thr Pro Ser Ser Ser Ser Ser Thr Pro Ser Ala Ala
545 550 555 560
Pro Ala Asn Gly Ser Ser Asp Asp Asp Arg Ser Ser Ser Gly Arg Arg
565 570 575
Thr Ala Ala Ala Val Asn Trp Arg Glu Glu Leu Ala Ala Leu Arg Ala
580 585 590
Gly Arg Glu Asp Ala Glu Glu Pro Ala Ser Ala Ser Ala Ser Tyr Asp
595 600 605
Arg Glu Phe Pro Ser Ser Ser Trp Ser Phe Ser Ser Ala Ser Ser Ala
610 615 620
Ala Val Val Gln Ser Gly Asp Ala Glu Asp Glu Ala Arg Arg Arg Phe
625 630 635 640
Gly Gly Leu Ala Gly Arg Gly Ala Arg Ser Asp Thr Thr Thr Ser Ala
645 650 655
Ala Ala Val Met Arg Gly Asn Gly Asn Gly Leu Ser Glu Asn Gly Tyr
660 665 670
Gly Asn Gly Tyr Gly Asn Asp Asn Gly Asn Gly Asn Gly Asn Thr Val
675 680 685
Glu Ala Arg Gly Ala Arg Pro Arg Thr Arg Pro Asp Trp Arg Asn Gln
690 695 700
Leu
705
<210> 62
<211> 403
<212> PRT
<213> Volvox carteri f. nagariensis
<400> 62
Met Ser Ser Ser Met Met Val Cys Gly Arg Gln Gln Arg Leu Ser Met
1 5 10 15
Pro Arg Gly Leu Gly Ser Ala Leu Pro Gly Ser Arg Leu Cys Arg Ser
20 25 30
Arg Leu Ile Ile Val Ala Ser Ala Ala Lys Asp Pro Asn Ala Pro Ile
35 40 45
Gln Ser Asn Pro Leu Gly Ala Leu Ser Ser Gln Ala Gly Thr Val Thr
50 55 60
Pro Leu Pro Arg Ser Glu Glu Ala Arg Lys Tyr Phe Arg Thr Val Tyr
65 70 75 80
Asp Phe Pro Gln Trp Gln Thr His Arg Ser Gln Phe Arg Leu Met Lys
85 90 95
Arg Leu Phe Thr Ile Pro Gln Ser His Val Ile Gln Asn Ala Leu Pro
100 105 110
Ser Ile Leu Trp Val Gly Ile Val Ser Ser Ala Leu Ala Ala Tyr Met
115 120 125
Thr Ala His Asp Gln His Ile Leu Pro Glu Gly Phe Pro Ser Leu Ser
130 135 140
Pro Asn Ala Ser Cys Ser Ala Phe Ile Ser Asn Thr Ser Val Ala Leu
145 150 155 160
Ser Leu Leu Leu Val Phe Arg Thr Asn Ser Ser Tyr Gly Arg Trp Asp
165 170 175
Glu Ala Arg Lys Met Trp Gly Gly Leu Leu Asn Arg Ser Arg Asp Ile
180 185 190
Met Arg Gln Gly Ala Thr Cys Phe Pro Asp Asp Gln Val Glu Ala Lys
195 200 205
Lys Ala Leu Ala Arg Trp Val Val Ala Phe Ala Arg Ala Leu Arg Ile
210 215 220
His Phe Gln Pro Glu Val Ser Ile Glu Ser Glu Leu Lys Asn Ile Leu
225 230 235 240
Thr Pro Ala Glu Leu Glu Met Leu Ala Lys Ser Gln His Arg Pro Val
245 250 255
Arg Ala Ile His Ala Ile Ser Gln Ile Ile Gln Ser Val Pro Met Ser
260 265 270
Ser Ile His Gln Met Gln Met Ser Asn Asn Leu Thr Phe Phe His Asp
275 280 285
Val Leu Gly Gly Cys Glu Arg Leu Leu Arg Ala Pro Ile Pro Val Ser
290 295 300
Tyr Thr Arg His Thr Ala Arg Phe Leu Phe Met Trp Leu Thr Leu Leu
305 310 315 320
Pro Phe Ala Leu Tyr Gly Gln Cys Gly Leu Gly Val Val Pro Val Cys
325 330 335
Thr Gly Ile Ala Ala Val Leu Cys Gly Ile Glu Glu Ile Gly Val Gln
340 345 350
Cys Glu Glu Pro Phe Gly Ile Leu Pro Leu Glu Val Ile Cys Asn Arg
355 360 365
Ile Gln Ala Asp Val Met Ala Thr Leu Lys Asp Asp Ala Asp Thr Lys
370 375 380
Thr Val Leu Ala Glu Ala Gly Leu Glu Arg Pro Gln Pro Gln Pro Gln
385 390 395 400
Arg Gly Glu
<210> 63
<211> 446
<212> PRT
<213> Volvox carteri f. nagariensis
<400> 63
Met Gln Ile Gly Thr Pro Met Arg Ala Ala Ala Ser Gln Arg Pro Leu
1 5 10 15
Gly Leu Arg Tyr Ser Cys Leu Pro Arg Lys Leu Ile Arg Val Val Ala
20 25 30
Asn Ala Ala Lys Asp Pro Asn Ala Pro Ile Gln Ser Asn Pro Leu Gly
35 40 45
Thr Leu Ser Ser Gln Ser Gly Thr Val Asn Pro Leu Pro Arg Ser Glu
50 55 60
Glu Ala Arg Arg Tyr Trp Arg Thr Val Tyr Asp Phe Pro Gln Trp Gln
65 70 75 80
Lys His Arg Ser Pro Tyr Arg Phe Met Glu Arg Leu Phe Gln Leu Ser
85 90 95
Gln Ser His Ile Leu Gln Asn Ala Leu Pro Ala Ile Ser Trp Val Thr
100 105 110
Leu Val Ala Thr Met Val Ala Ala Tyr Gly Thr Ser Tyr Asp Gln His
115 120 125
Leu Leu Pro Asp Gly Phe Pro Ser Ile Ser Pro Asn Ala Ser Cys Ser
130 135 140
Ala Phe Val Ser Asn Thr Ser Val Ala Leu Ser Leu Leu Leu Val Phe
145 150 155 160
Arg Thr Asn Ser Ser Tyr Gly Arg Trp Asp Glu Ala Arg Lys Met Trp
165 170 175
Gly Gly Leu Leu Asn Arg Ser Arg Asp Ile Met Arg Gln Gly Ala Thr
180 185 190
Cys Phe Pro Asp Asp Gln Val Glu Ala Lys Lys Ala Leu Ala Arg Trp
195 200 205
Val Val Ala Phe Ala Arg Ala Leu Arg Ile His Phe Gln Pro Glu Val
210 215 220
Thr Ile Glu Ser Glu Leu Lys Asn Ile Leu Thr Pro Ala Glu Leu Glu
225 230 235 240
Met Leu Ala Lys Ser Gln His Arg Pro Val Arg Ala Ile His Ala Ile
245 250 255
Ser Gln Ile Ile Gln Ser Val Pro Met Ser Ser Ile His Gln Met Gln
260 265 270
Met Ser Asn Asn Leu Thr Phe Phe His Asp Val Leu Gly Gly Cys Glu
275 280 285
Arg Leu Leu Arg Ala Pro Ile Pro Val Ser Tyr Thr Arg His Thr Ala
290 295 300
Arg Phe Leu Phe Ala Trp Leu Thr Leu Leu Pro Phe Ala Leu Tyr Pro
305 310 315 320
Ser Ala Gly Trp Gly Val Val Pro Val Cys Thr Gly Ile Ala Ala Val
325 330 335
Leu Cys Gly Ile Glu Glu Ile Gly Val Gln Cys Glu Glu Pro Phe Gly
340 345 350
Ile Leu Pro Leu Asp Val Ile Cys Asn Arg Ile Gln Ala Asp Val Met
355 360 365
Ala Thr Leu Lys Asp Asp Ala Asp Thr Lys Thr Val Leu Ala Glu Ala
370 375 380
Gly Leu Ala Ala Thr Gly Ala Ala Val Ala Ala Pro Arg Trp Leu Met
385 390 395 400
Arg Arg Ala Pro Arg Ser Gly Trp Glu Glu Gly Ala Arg Glu Leu Val
405 410 415
Val Cys Arg Arg Ala Arg Ile Thr Ser Ser Ser Ser Asp Ser Glu Cys
420 425 430
Glu Val Val Ala Ser Pro Cys Val Arg Phe Leu Ile Glu Pro
435 440 445
<210> 64
<211> 398
<212> PRT
<213> Volvox carteri f. nagariensis
<400> 64
Met Gln Ala Asp Leu Ala Gly Phe His Ala Arg Pro Thr Phe Lys Arg
1 5 10 15
Thr Arg Asn Asn Cys Leu Arg His Ala His Arg Lys Pro Pro Phe Ala
20 25 30
Arg Pro Val Ala Phe Gly Ser Pro Ile Gln Pro Leu Asp Arg Ser Ser
35 40 45
Leu Trp Thr Ser Ser Ala Leu Trp Thr Val Glu Ser Val Leu Val Asn
50 55 60
Leu Arg Arg Lys Arg Phe Glu Pro Lys Thr Val Glu Arg Val Tyr Glu
65 70 75 80
Ala Asp Val Tyr Pro Ala Val Phe Asp Phe Ala Ser Trp Thr Gln His
85 90 95
Arg Ser Arg Gly Arg Tyr Leu Glu His Cys Ile Thr Leu Phe Arg Ser
100 105 110
Tyr Phe Phe Arg Asp Leu Leu Gly Pro Leu Leu Val Leu Val Gly Ala
115 120 125
Ala Met Thr Val Ala Ser Tyr Glu Thr Ala Leu Gln Thr Gly Leu Leu
130 135 140
Pro Arg Trp Leu Pro Asp Phe Gly Gly Ser Ser Asp Thr Pro Phe Gln
145 150 155 160
Leu Thr Ser Phe Ala Leu Ser Leu Met Leu Val Phe Arg Thr Asn Ser
165 170 175
Ser Tyr Ala Arg Trp Leu Asp Ala Arg Gln Gln Trp Gly Leu Ile Val
180 185 190
Asn Thr Ala Arg Thr Phe Val Arg Gln Val Met Thr Thr Leu Pro Glu
195 200 205
Thr Ser Cys Cys Glu Val Arg Ser Ala Leu Ala Arg Trp Thr Val Ala
210 215 220
Phe Val Arg Leu Gly Lys Leu His Leu Arg Glu His Gly Asp Val Gly
225 230 235 240
Ala Glu Val Gln Gly Leu Leu Asp Gly Glu Glu Val Pro Leu Val Leu
245 250 255
Ala Ala Ser His Arg Pro Leu Ala Ala Cys His Val Met Ser Gly Leu
260 265 270
Leu Arg Ser Ala Glu Gly Ser Gly Gln Leu Ser Glu Gln Ala Arg Met
275 280 285
Arg Leu Glu Ala Asp Ile Asn Leu Met Ser Gln Ala Leu Gly Ala Cys
290 295 300
Glu Lys Ile Leu Arg Asn Pro Ile Pro Leu Ser Tyr Thr Arg His Thr
305 310 315 320
Ser Arg Phe Leu Ile Leu Trp Leu Leu Trp Leu Pro Leu Ala Leu Trp
325 330 335
Gly Lys Val Gly Trp Cys Val Val Pro Val Glu Ala Val Met Thr Tyr
340 345 350
Leu Leu Leu Gly Ile Asp Glu Ile Ala Val Gln Met Glu Glu Pro Phe
355 360 365
Gly Gly Ser Gly Leu Gly Gly Ala Gly Arg Ser Ala Ala Ser Cys Arg
370 375 380
Trp Lys Thr Cys Val Arg Leu Tyr Asn Asn Pro Trp Ser Asn
385 390 395
<210> 65
<211> 389
<212> PRT
<213> Volvox carteri f. nagariensis
<400> 65
Met Ser Thr Asp Phe Val Ala Tyr Ser Val Cys Thr Ser Ala Leu Val
1 5 10 15
Cys Val Thr Phe Thr Phe Asp Asp Trp Lys Arg His Arg Ser Ser Asn
20 25 30
Arg Tyr Leu Tyr His Leu Lys Thr Leu Ser Glu Ser Gly Ile Val Arg
35 40 45
Gly Ile Ala Ala Pro Val Leu Phe Val Thr Ala Phe Thr Ala Gly Met
50 55 60
Thr Leu Leu Tyr Val Val Ala Ala Leu Asn Val Ala His Ala Ala Arg
65 70 75 80
Met Leu Pro Ala Trp Leu Pro Pro Leu Pro Asn Ile Ala Ile Glu Pro
85 90 95
Ile Gln Leu Thr Ser Ile Ala Leu Ser Leu Leu Leu His Met Leu Thr
100 105 110
Gly Val Arg His Glu Ser Ser Glu Thr Pro Pro Lys Pro Arg His Pro
115 120 125
Arg Leu Leu Thr Cys Arg Arg Phe Pro Cys Ala Pro Leu Ser Leu Pro
130 135 140
Val Leu Phe Tyr Asp Met Glu Lys Val Phe Arg Thr Asn Ala Ser Tyr
145 150 155 160
Ser Arg Trp Asp Glu Gly Arg Arg Ser Phe Gly Ser Ile Thr Thr Val
165 170 175
Ser Arg Asp Ile Ala Arg Gln Ala Phe Ala Trp Phe Arg Thr Asp Asp
180 185 190
Met Thr Asn Arg Ser Arg Leu Gly Arg Trp Leu Val Ala Leu Gly Arg
195 200 205
Val Thr Met Val His Leu Arg Glu Glu His Gly Met Gln Glu Glu Leu
210 215 220
Lys Gly Val Leu Lys Pro Gln Glu Ile Asp Ala Val Thr Ser Ser Ile
225 230 235 240
His Ala Pro Ser Phe Cys Leu Gln Met Ile Thr Trp Thr Ile Arg Thr
245 250 255
Ala Gly Leu Pro Gln Glu Leu Val Ile Arg Met Asp Glu Asn Val Ser
260 265 270
Arg Leu Thr Asp Ala Val Ser Ala Cys Glu Arg Ile Leu Asn Thr Pro
275 280 285
Ile Pro Leu Ser Tyr Thr Arg His Thr Ala Arg Phe Leu Met Ala Trp
290 295 300
Leu Val Cys Leu Pro Phe Cys Leu Trp Ser Tyr Cys Gly Leu Ala Met
305 310 315 320
Val Pro Ile Ala Ala Leu Val Ala Phe Val Leu Leu Gly Ile Glu Glu
325 330 335
Ile Gly Val Tyr Ile Glu Glu Pro Phe Ser Ile Leu Ala Leu Glu Lys
340 345 350
Leu Val Asn Lys Leu Glu Asn Ile Val Asn Ala Met Leu Arg Glu Ser
355 360 365
Gln Trp Gln Thr Gln Ser Phe Ala Leu His Ile Leu Phe Val Gly Pro
370 375 380
Phe Pro Val Ser Val
385
<210> 66
<211> 929
<212> PRT
<213> Micractinium conductrix
<400> 66
Met Gln Arg Val Leu Ala Ala Ser Gly Ser Thr Thr His Met Ala Ala
1 5 10 15
Arg Pro Gly Leu Ser Gly Ser Ser Arg Ala Arg Ala Ala Thr Ala Ala
20 25 30
Ala Thr Ala Arg Leu Thr Pro Ala Gly Arg Gly Asn Ala Leu Arg Leu
35 40 45
Ala Met Pro Gln His Ala Gly Ala Ala Ala Ala Gly Pro Arg Thr Arg
50 55 60
Ala Pro Val Arg Val Ser Ala Ala Ala Asp Pro Ala Val Lys Leu Thr
65 70 75 80
Gly Asp Asp Leu Lys Glu Ala Asn Arg Lys Gln Met Arg Thr Val Phe
85 90 95
Asp Phe Glu Leu Trp Arg Lys His Arg Ser Ser Ala Arg Tyr Trp Arg
100 105 110
His Ile Thr Gly Ile Phe Glu Ser Arg Ile Val Ser Gly Leu Ala Ala
115 120 125
Pro Leu Gly Tyr Val Ala Ala Leu Ser Thr Thr Val Ala Leu Tyr His
130 135 140
Val Leu Ala Glu Ala Gly Tyr Ile Met Asp Val Pro Asp Leu Lys Leu
145 150 155 160
Ala Ser Asn Ala Pro Phe Gly Leu Thr Ser Phe Ala Leu Ser Leu Leu
165 170 175
Leu Val Phe Arg Thr Asn Ser Ser Tyr Gly Arg Trp Asp Glu Ala Arg
180 185 190
Lys Met Trp Gly Leu Ile Val Asn Arg Ser Arg Asp Phe Val Arg Gln
195 200 205
Gly Leu Gly Tyr Ile Pro Asp Ser Gln Pro Glu Leu Gln Asn Met Leu
210 215 220
Cys Arg Trp Thr Val Ala Tyr Ser Arg Ser Leu Met Cys His Leu Arg
225 230 235 240
Pro Gly Glu Asn Leu Glu Glu Glu Leu Lys Glu Thr Leu Pro Ala His
245 250 255
Glu Leu Lys Ala Leu Leu Ser Ser Thr His Arg Pro Asn Tyr Val Val
260 265 270
Gln Val Leu Thr Ala Val Leu Lys Glu Ala Gln Leu Pro Thr Ala Val
275 280 285
Thr Ser Ser Arg Asp Ser Thr Ala Val Val Pro Ala Gly Ala Ala Tyr
290 295 300
Arg Met Asp Glu Asn Leu Thr Val Phe Ala Asp Val Thr Gly Gly Cys
305 310 315 320
Glu Arg Ile Leu Arg Thr Pro Ile Pro Leu Ser Tyr Thr Arg His Thr
325 330 335
Ser Arg Phe Met Met Ile Trp Leu Thr Leu Leu Pro Phe Thr Leu Trp
340 345 350
Asp Ser Cys Arg Trp Ala Met Val Pro Ile Ser Leu Ile Val Ser Phe
355 360 365
Leu Leu Leu Gly Ile Glu Glu Ile Gly Val Ser Ile Glu Glu Pro Phe
370 375 380
Thr Ile Leu Pro Leu Glu Val Ile Ser Arg Thr Ile Glu Gly Asn Val
385 390 395 400
Lys Glu Leu Gln Lys Met His Ser Ser Glu Gly Lys Ala Val Met Gly
405 410 415
Ala Ser Ala Glu Gly Asp Asn Leu Ser Ala Gln Ala Leu Val Asp Ala
420 425 430
Val Leu Pro Ala Ile Ser Gly Asn Gly Asn Gly Gly Ala Leu Ser Ala
435 440 445
Ala Gly Ser Ser Ser Gly Ser Ser Arg Ile Val Ile Lys Pro Ala Met
450 455 460
Ala Met Gln Gln Arg Ala Ser Ala Ala Pro Lys Arg Ser Ala Ala Thr
465 470 475 480
Gly Ala Arg Ala Leu Val Ala His Pro Ala Ala Cys Arg Cys Ala Ala
485 490 495
His Arg Arg Gly Arg Ser Leu Val Val Arg Ala Ala Ser Asp Asn Val
500 505 510
Met Glu Val Gly Gly Arg Thr Ile Val Val Glu Asp Asp Gly Thr Ile
515 520 525
Ile Ile Ser Gln Pro Ala Ala Ser Ala Ala Val Asp Val Lys Glu Ser
530 535 540
Ala Glu Val Glu Tyr Leu Thr Ala Arg Ser Ala Ile Val Asn Lys His
545 550 555 560
Phe Glu Gly Ser Leu Gly Ala Asp Asp Phe Ile Gln Arg Val Glu Met
565 570 575
Ala Leu Tyr Ala Phe Gly Phe Thr Gly Glu Asn Ser Ile Ala Met Val
580 585 590
Asn Leu Cys Arg Asp Glu Val Thr Val Thr Leu Lys Gln Lys Ile Asp
595 600 605
Ala Val Phe Gly Ala Ser Phe Ser Thr Asn Gly Leu Gly Gly Val Leu
610 615 620
Thr Cys Gly Ala Val Gly Met Gly Ala Gly Phe Ser His Ser Pro Ile
625 630 635 640
Cys Asp Thr Thr Gly Lys Glu Arg Tyr Ile Phe Phe Ser Phe Pro His
645 650 655
Ile Ser Ile Asn Ser Lys Gly Glu Val Gly Pro Met Ser Arg Pro Gly
660 665 670
Arg Pro Gly Gln Ser Cys Ala Cys Gly Ala Leu Ile Lys Ser Trp Thr
675 680 685
Glu Leu Arg Ser Glu Gly Val Ser Cys Asn Cys Lys Ile Pro Gly Val
690 695 700
His Asp Ala Glu Asn Pro Glu Tyr Ser Ile Leu Lys Gln Arg Ile Ala
705 710 715 720
Arg Arg Leu Arg His Glu Gly Glu Thr Asp Glu Ser Val Gln Gln Leu
725 730 735
Ser Leu Val Asp Ile Thr Lys Val Ala Glu Arg Thr Ile Ser Asp Asp
740 745 750
Leu Glu Tyr Leu Ile Ser Lys Thr Val Asp Thr Asn Lys Ala Asp Tyr
755 760 765
Ala Val Ile Thr Gly Val Gln Val His Asn Trp Ala Phe Asp Phe Glu
770 775 780
Asp Asp Ser Pro Asn Leu Glu Phe Val Phe Pro Thr Ser Ala Tyr Val
785 790 795 800
Val Val Asp Gly Val Lys Thr His Val Asp Leu Ala Ala Met Pro Pro
805 810 815
Leu Thr Pro Arg Gln Ile Arg Leu Val His Gly Gly Asp Gln Gly Val
820 825 830
Val Cys Asn Thr Gly Gly Gln Ser Thr Leu Arg Ala Glu Asp Pro Pro
835 840 845
Tyr Ala Tyr Asp Ser Lys Asp Ala Arg Lys Val Gln Arg Thr Arg Leu
850 855 860
Gln Asn Tyr Ile Ser Leu Ile Lys Asp Glu Gly Leu Glu Ser Val Ala
865 870 875 880
Ala Ala Pro Ser Ser Pro Ala Trp Ala Lys Gln Ile Thr Lys Ser Val
885 890 895
Ala Asp Arg Thr Asn Ser Thr Ala Leu Asp Met Lys Phe Ala Gln Asn
900 905 910
Glu Asp Leu Lys Glu Met Gln Ala Gln Leu Glu Ala Lys Tyr Gln Ser
915 920 925
Leu
<210> 67
<211> 250
<212> PRT
<213> 莱茵衣藻
<400> 67
Met Thr Arg Trp Arg Pro Arg Arg Pro Trp Pro Ala Gly Leu Val Ala
1 5 10 15
Phe Ala Arg Ala Leu Arg Ile His Phe Gln Pro Glu Val Thr Ile Glu
20 25 30
Ser Glu Leu Lys Asn Ile Leu Thr Pro Ala Glu Leu Gln Met Leu Ala
35 40 45
Lys Ser Gln His Arg Pro Val Arg Ala Ile His Ala Ile Ser Gln Ile
50 55 60
Ile Gln Ser Val Pro Met Ser Ser Ile His Gln Gln Gln Met Ser Asn
65 70 75 80
Asn Leu Thr Phe Phe His Asp Val Leu Gly Gly Cys Glu Arg Leu Leu
85 90 95
Arg Ala Pro Ile Pro Val Ser Tyr Thr Arg His Thr Ala Arg Phe Leu
100 105 110
Phe Ala Trp Leu Thr Leu Leu Pro Phe Ala Leu Tyr Gly Ser Cys Gly
115 120 125
Val Ser Val Ile Pro Val Cys Ser Gly Ile Ala Ala Val Leu Cys Gly
130 135 140
Ile Glu Glu Ile Gly Val Gln Cys Glu Glu Pro Phe Gly Ile Leu Pro
145 150 155 160
Leu Asp Val Ile Cys Asn Arg Ile Gln Ala Asp Val Met Ala Thr Leu
165 170 175
Lys Asp Asp Ala Asp Thr Lys Thr Ile Leu Ala Glu Ala Gly Leu Ile
180 185 190
Ser Leu Arg Ala Asn Ser Ala Met Ala Val Glu Asn Ala Leu Pro Asp
195 200 205
Leu Asp Ser Ile Asn Ala Ala Ala Pro Asn Gly Asn Gly Ser His Asn
210 215 220
Gly Asn Gly Ala Ala Val Pro Val Ser Val Ser Ala Gly Ala Ser Gly
225 230 235 240
Asn Gly Met Asn Val Arg Ile Ser Pro Arg
245 250
<210> 68
<211> 883
<212> PRT
<213> Micractinium conductrix
<400> 68
Met Arg Arg Arg Ala Leu Ala Ala Ala Ala Ala Ala Gly Ser Thr Ala
1 5 10 15
Pro Pro Pro His Arg Leu Gly Asp Asp Gly Ala Gly Asn Ala Ala Pro
20 25 30
Pro Pro Leu Asp Pro Ser Met Pro Leu Pro Leu Pro Trp Gln Ala Tyr
35 40 45
Phe Asp Gly Arg His Ala Ala Ser Cys Pro Gly Arg Gly Ala Thr Phe
50 55 60
Asn Val Tyr Thr Ala Gly Ser Ala Gly Pro Val Val Leu Cys Leu His
65 70 75 80
Gly Gly Gly Tyr Thr Gly Leu Ser Trp Ser Leu Ile Ala Arg Ala Leu
85 90 95
Lys Asp Lys His Arg Val Val Ala Pro Asp Leu Arg Cys His Gly Leu
100 105 110
Thr Val Ala Glu Asp Glu Ala Asp Phe Ser Ala Asp Thr Leu Ala Gly
115 120 125
Asp Val Val Ala Leu Trp Arg Ala Leu Phe Ala Gly Gly Gly Ala Ala
130 135 140
Gly Ser Asp Gly Ser Thr Gly Ser Gly Gly Gly Gly Gly Gly Ser Ser
145 150 155 160
Glu Ala Asp Val Gln Gln His Leu Gln Arg Leu Arg Gln Gly Pro Pro
165 170 175
Pro Pro Thr Val Leu Val Gly His Ser Met Gly Gly Ala Ile Ala Val
180 185 190
His Ala Ala Ala Leu Gly Gly Ile Thr Ser Leu Ala Gly Ile Val Val
195 200 205
Ile Asp Val Val Glu Gly Thr Ala Leu Ala Ser Leu Pro Tyr Met Ala
210 215 220
Gly Val Leu Glu Lys Arg Pro Arg Arg Phe Ala Ser Leu Gln Gln Ala
225 230 235 240
Val Asp Trp Ala Val Asp Ser Gly Met Cys Lys Arg Gln Glu Ala Ala
245 250 255
His Val Ser Leu Pro Ala Met Leu Arg Arg Glu Pro Pro Gly Gly Pro
260 265 270
Thr Gly Gly Gly Ala Glu Ala Ala Gly Gly Val Val Arg Leu Val Pro
275 280 285
Gly Gly Leu Ala Pro Leu Ala Glu Glu Gly Glu Glu Glu Glu Asp Gly
290 295 300
Ala Glu Ala Ala Ala Ala Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln
305 310 315 320
Gln Gln Gln Gln Val Gln Gln Pro Ala Ala Gly Thr Gln Gln Ala Ala
325 330 335
Pro Ser Gly Gly Gly Gly Gly Gly Gly Gly Trp Val Trp Arg Thr Pro
340 345 350
Leu Glu Arg Ser Ala Pro Phe Trp Glu Gly Trp Tyr Gln Gly Leu Ser
355 360 365
Asp Met Phe Leu Lys Leu Ser Val Pro Lys Val Leu Val Leu Val Gly
370 375 380
Thr Asp Arg Leu Asp Arg Pro Leu Thr Ile Gly Gln Met Gln Gly Arg
385 390 395 400
Phe Gln Pro Val Leu Leu Pro Gln Ala Gly His Ala Val His Glu Asp
405 410 415
Glu Pro Glu Ala Thr Ala Asp Ala Ile Ala Ala Phe Ile Lys Arg Phe
420 425 430
Arg Asn Gly Glu Pro Pro Leu Gln Ile Pro Arg Pro Thr Pro Gly Leu
435 440 445
Arg Pro Val Leu Pro Val Ala Met Gly Pro Ala Asp Ser Leu Arg Val
450 455 460
Gln Ser Lys Pro Asp Arg Ser Ile Asp Arg Ala Ser Ser Ser Asp Lys
465 470 475 480
Val Val Ser Phe Pro Ala Leu Asp Ser Ile Glu Arg Tyr Tyr Glu Pro
485 490 495
Val Lys Glu Asp Ala Arg Gln Tyr Arg Arg Thr Val Phe Asp Phe Glu
500 505 510
Lys Trp Arg Glu His Arg Ser Thr Lys Arg Tyr Ala Arg His Ile Lys
515 520 525
Ser Ile Lys Gly Ser Arg Met Val Arg Gly Leu Ala Ala Pro Leu Leu
530 535 540
Trp Leu Thr Ala Val Ala Ala Ala Val Ala Thr Phe Asn Thr Leu Val
545 550 555 560
Glu Ala Gly Leu Ala Pro Asp Val Leu Pro Glu Leu Lys Phe Ser Asn
565 570 575
Asn Gly Pro Phe Gly Leu Thr Ser Phe Ala Leu Ser Leu Leu Leu Val
580 585 590
Phe Arg Thr Asn Ala Ser Tyr Ala Arg Trp Leu Asp Ala Arg Lys Asn
595 600 605
Trp Gly Thr Leu Thr Asn Arg Ser Arg Asp Leu Thr Arg Gln Ala Leu
610 615 620
Thr Phe Phe Pro Ala Glu Asp Pro Ala Leu Phe His Leu Leu Cys Arg
625 630 635 640
Trp Val Gln Ala Tyr Ser Arg Ser Leu Met Cys His Leu Arg Asp Asp
645 650 655
Val Ser Ile Glu Glu Glu Leu Pro Lys Ala Leu Leu Pro Tyr Glu Val
660 665 670
Glu Ala Val Leu Ala Ser Arg His Arg Pro Leu Tyr Cys Leu Gln Met
675 680 685
Leu Ser Glu Ile Val Arg Ala Ala His Val Pro Pro Ala Gly Gln Pro
690 695 700
Pro Ala Gly Ala Ala Ala Ala Ala Val Ala Ala Ala Ala Asp Pro Phe
705 710 715 720
Ala Gly Ala Ala Leu Ala Ala Ala Leu Arg Ala Ala Ala Ala Gln Arg
725 730 735
Met Asp Glu Asn Leu Ser Ile Phe Glu Asp Val Cys Gly Thr Cys Glu
740 745 750
Arg Ile Leu Arg Ala Pro Ile Pro Leu Ser Tyr Thr Arg His Thr Ser
755 760 765
Arg Phe Met Thr Ile Tyr Leu Ser Leu Leu Pro Phe Thr Leu Trp Asp
770 775 780
Ser Ala Gly Trp Ala Met Val Pro Leu Ile Ala Ile Val Ala Phe Leu
785 790 795 800
Leu Leu Gly Ile Glu Glu Ile Gly Val Ser Ile Glu Glu Pro Phe Ser
805 810 815
Ile Leu Pro Leu Glu Gln Met Cys Asp Thr Ile Gln Ala Ser Val Val
820 825 830
Glu Leu Arg Ala Ile Asn Ser Gln Gln His Glu Gly Ser Ser Gly Asp
835 840 845
Gly Gly Asn Gly Asn Gly Val Ala Ala Ala Pro Arg Gln Pro Pro Ser
850 855 860
Ala Arg Ala Leu Leu Arg Arg Gly Val Ala Glu Ala Ala Ala Ala Asp
865 870 875 880
Ala Leu Ala
<210> 69
<211> 2445
<212> PRT
<213> 小球藻
<400> 69
Met Gly Gly His Gly Phe Ile Glu Gly Phe Leu Lys Ser Trp Gly Val
1 5 10 15
Ile Leu Ala Ser Glu Ile Gly Asp Lys Thr Phe Phe Ile Ala Ala Ile
20 25 30
Met Ala Met Arg His Ala Arg Leu Thr Val Phe Ala Gly Ala Met Ala
35 40 45
Ala Leu Ala Ala Met Thr Val Leu Ser Ala Ala Leu Gly Trp Ala Ala
50 55 60
Pro Asn Leu Ile Ser Lys Thr Tyr Thr His Tyr Ala Ala Thr Ala Leu
65 70 75 80
Phe Phe Phe Phe Gly Gly Arg Met Leu Tyr Glu Ala Val Thr Asn Ala
85 90 95
His Ala Gly Glu Asn Glu Leu Asp Glu Val Glu Lys Glu Leu Glu Ala
100 105 110
Val Asp Lys Ser Pro Lys Thr Gly Gly Pro Ala Ala Asn Gly Lys Asn
115 120 125
Pro Leu Leu Gly Thr Leu Arg Arg Phe Val Ser Pro Val Leu Leu Glu
130 135 140
Ala Phe Thr Leu Thr Phe Leu Ala Glu Trp Gly Asp Arg Ser Gln Ile
145 150 155 160
Ala Thr Ile Gly Leu Ala Ala Ala Ala Asp Val Phe Gly Val Thr Val
165 170 175
Gly Gly Ile Leu Gly His Ala Met Cys Thr Gly Ala Ala Val Leu Gly
180 185 190
Gly Lys His Leu Ala Glu His Ile Asp Glu Arg Lys Val Ala Tyr Phe
195 200 205
Gly Gly Val Leu Phe Leu Leu Phe Gly Ala His Ser Leu Tyr Thr Gly
210 215 220
Val Pro Glu Cys Gly Gly Glu Ala Lys Met Pro Ser Leu Ala Ala Thr
225 230 235 240
Glu Pro Val Asp Tyr Ala Val Leu Gly Ala Ala Gly Ala Ala Ala Leu
245 250 255
Ala Gly Leu Ala Ala Cys Leu Tyr Thr Val Pro Trp Arg Leu Cys Arg
260 265 270
Ala Ala Ala Pro Pro Ser Ala Pro Lys Ala Thr Arg Ser Phe Asn Val
275 280 285
Leu Trp His Gly Arg Ser Leu Leu Ala Leu Leu Leu Thr Ala Trp Ala
290 295 300
Leu Ser Pro Leu Leu Arg Val Ser Arg Leu Trp Gly Ala Asn Ser Phe
305 310 315 320
Val Phe Ser Asp Gly Val Thr Ser Trp Thr Gly Ser Gly Trp Met Cys
325 330 335
Arg Ile Tyr Leu Thr Ala Ala Leu Gly Val Leu Gln Pro Leu Cys Ala
340 345 350
Phe Leu Ala Leu Leu Met Leu Thr Ala Gly Leu Ser Arg Arg His Arg
355 360 365
Pro Glu Ala Leu Arg His Pro Asn Ala Arg Leu Leu Gly Leu Ala Ala
370 375 380
Leu Cys Thr Leu Pro Val Ala Ala Val Gln Val Val Val Ala Trp Leu
385 390 395 400
Ser Leu Ala Val Gln Tyr Gln Gly Gln Pro Leu Glu Gln Ala Pro Arg
405 410 415
Ser Val Leu Gly Tyr Phe Phe Ala Thr Tyr Trp Glu Gly Ser Pro Glu
420 425 430
Gln Cys Gly Thr Gln Ala Ala Ala Ala Ala Ala Val Val Pro Ala Ala
435 440 445
Ala Asn Asp Ala Ala Ala Leu Gln Thr Pro Gly Gly Ser Gly Ser Leu
450 455 460
Ser Leu Ser Ala Ala Gly Ser Pro Gly Asp Ala Ala Ala Ala Gly Ala
465 470 475 480
Ala Val Ala Pro Asp Gly Gly Cys Thr Leu Cys Cys Phe Pro Ala Ala
485 490 495
Ala Val Ala Val His Ala Ile Phe Thr Val Ala Phe Leu Ala Ala Leu
500 505 510
Trp Val Thr Cys Ser Arg Leu Ala Asp Ala Val Leu Asn Ser Arg Leu
515 520 525
Lys Arg Arg Met Arg Leu Phe Gln Leu Ala His Ser Leu Leu Ala Val
530 535 540
Gly Gly Val Ala Ala Leu Gly Val Ser Ile Val Gln Gly Pro Phe Thr
545 550 555 560
Trp Leu Asn Gln Ala Cys Trp Ala Ala Tyr Val Gly Thr Ala Val Ala
565 570 575
Thr Val Ala Leu Val Leu Trp Glu Val Val Val Ser Pro Val Arg Asp
580 585 590
Leu Arg Ser Val Asp Lys Arg Leu Ala Leu Trp Ala Asp Ala Thr Thr
595 600 605
Ala Ala Phe Ala Ala Ala Gly Ala Asp Glu Pro Pro Met Ser Thr Gln
610 615 620
Ser Ser Gly Gly Ser Ser Val Leu Ile Lys Ala His Asn Arg Ser Ala
625 630 635 640
Glu Ala Glu Ala Tyr Val Gln Ala Ala Ala Ala Ala Arg Thr Leu Ser
645 650 655
Ala Ser Pro Ala Gln Gly Gln Glu Lys Gly Gln Pro Ala Pro Pro Arg
660 665 670
Pro Ser Leu Gln Leu Ala Pro Leu Ala Leu Pro Pro Leu Pro Gln Gly
675 680 685
Thr Pro Arg Arg Ser Pro Ala Lys Gln Pro Thr Pro Leu Trp Arg Gln
690 695 700
Gln Ala Gln Pro Ser Leu Leu Arg Gln Gln Tyr Gly Thr Ala Pro Ser
705 710 715 720
Pro Leu Val Ser Pro Pro Arg Gln Pro Ala Arg Val Thr Val Ala Gly
725 730 735
Ala Ala Ala Asp Leu Pro Pro Pro Gly Pro Val Thr Ala Gly Leu Lys
740 745 750
Ala Ala Gln Arg Arg Gln Pro Gly Ser Leu Arg Gly Lys Gln Glu Asp
755 760 765
Val Trp Ser Leu Glu Asp Ala Ala Glu Met Gly Ala Ala Cys Ser Asn
770 775 780
Ser Glu Lys Glu Leu Gly Arg Arg Tyr Met Arg Ser Leu Ser Thr Phe
785 790 795 800
Asn Phe Gln Arg Trp Ala Phe His Arg Ser Thr Asp Arg Tyr Ala Arg
805 810 815
His Met Leu Gly Ile Phe Gln Ser Arg Ile Val Arg Gly Leu Ala Ala
820 825 830
Pro Leu Leu Ser Ala Gly Ala Thr Ala Thr Leu Val Cys Leu Tyr Glu
835 840 845
Gln Ala Leu Gln Gln Gly Ala Leu Pro Ala Gln Leu Pro Ser Leu Val
850 855 860
Ile Ser Pro Leu Pro Leu Asp Leu Thr Ser Ala Ala Leu Gly Leu Leu
865 870 875 880
Leu Val Phe Arg Thr Asn Ser Ser Tyr Glu Arg Trp Gln Gln Ala Leu
885 890 895
Ala Ala Trp Gly Ala Ile Thr Thr Arg Ser Arg Asp Thr Met Arg Gln
900 905 910
Leu Leu Cys Tyr Thr Ala Ala Thr Pro Glu Ala Gly Pro Ala Ser Thr
915 920 925
Lys Leu Leu Thr Val Ala Ala Thr Gly Arg Trp Leu Val Ala Phe Ala
930 935 940
Arg Ala Leu Lys Ala Gln Leu Thr Glu Asp Ser Asp Leu Arg Ala Glu
945 950 955 960
Leu Glu Gly Ile Leu Thr Pro Thr Glu Leu Asp Leu Leu Cys Thr Ser
965 970 975
Tyr Gln Pro Ser Ser Phe Thr Leu Ser Val Leu Thr Glu Leu Val Ala
980 985 990
Ala Ala Pro Leu Arg Asp Ser Gln Arg Ile Arg Val Asp Glu Asn Leu
995 1000 1005
Thr Ala Cys Glu Asp Ala Met Ser Ala Cys Glu Arg Ile Leu Arg Thr
1010 1015 1020
Pro Ile Pro Leu Ser Tyr Thr Arg His Thr Ser Arg Phe Met Val Ile
1025 1030 1035 1040
Trp Leu Ser Cys Leu Pro Leu Gly Leu Trp Pro Ala Val Gly Trp Gly
1045 1050 1055
Thr Val Pro Leu Thr Val Ala Ile Ala Phe Leu Leu Leu Gly Ile Glu
1060 1065 1070
Glu Ile Gly Val Ala Val Glu Glu Pro Phe Ser Ile Leu Pro Leu Asp
1075 1080 1085
Glu Leu Cys Arg Glu Ile Glu Ile Ser Leu Ser Asp Ile Val Glu Gln
1090 1095 1100
Ala Ser Ser Ser Lys Arg Ala Ala Lys Glu Ala Val Ser Ala Ala Ala
1105 1110 1115 1120
Ala Ala Ala Val Thr Ala Ala Ala Ala Glu Ala Ala Ala Ala Ala Ala
1125 1130 1135
Ala Ala Ala Ala Glu Ala Ala Asp Gly Ser Gly Ser Ser Thr Ser Ser
1140 1145 1150
Gly Asp Ser Thr Pro Leu Tyr Ala Ala Ala Ala Gln Ala Pro Ala Ala
1155 1160 1165
Ala Asn Ala Val Ala Pro Leu Leu Pro Gly Ala Leu Gly Arg Ala Ser
1170 1175 1180
Pro Leu Lys Met Ala Ala Lys Asp Leu Glu Ser Gly Asp Ala Ser Thr
1185 1190 1195 1200
Ser Ser Asp Gly Cys Arg Leu Glu Pro Ser Pro Lys Lys Gly Leu Lys
1205 1210 1215
Gln Ser Leu Ser Arg Ala Phe Leu Ser Ser Gly Leu Gln Val Thr Phe
1220 1225 1230
Lys Asp Ile Thr Tyr Thr Val Val Asn Ser Gln Asn Lys Lys Glu Lys
1235 1240 1245
Leu Asp Leu Leu Lys Gly Val Gly Gly Tyr Leu Gln Ala Gly Glu Met
1250 1255 1260
Ala Ala Leu Met Gly Pro Ser Gly Ser Gly Lys Ser Thr Leu Leu Asp
1265 1270 1275 1280
Val Leu Ser Gly Arg Lys Thr Val Gly Glu Leu Gln Gly Glu Val Ala
1285 1290 1295
Phe Ala Gly Asn Arg Pro Thr Pro Gln Phe Leu Arg Arg Phe Thr Gly
1300 1305 1310
Tyr Val Glu Gln Phe Asp Thr Leu Leu Asp Ile Leu Thr Val Glu Glu
1315 1320 1325
Met Leu Met Tyr Thr Ala Glu Leu Lys Arg Pro Val Ser Gln Thr Leu
1330 1335 1340
Glu Gln Lys Lys Gly Ala Val Asp Glu Leu Ile Glu Val Leu Gly Leu
1345 1350 1355 1360
Asp Gly Cys Arg Thr Val Arg Ile Gly Asn Pro Met Ala Arg Gly Ile
1365 1370 1375
Ser Gly Gly Gln Ala Lys Arg Val Asn Ile Gly Ile Ala Leu Val Thr
1380 1385 1390
Asn Pro Arg Val Leu Phe Leu Asp Glu Pro Thr Ser Gly Leu Asp Ser
1395 1400 1405
Tyr Thr Ala Asn Glu Val Met Ser Val Val Lys Ser Leu Ala Ser His
1410 1415 1420
Gly Ile Thr Val Cys Ala Thr Ile His Ser Pro Thr Pro Tyr Thr Phe
1425 1430 1435 1440
Asn Leu Phe Asp Arg Leu Leu Leu Leu Leu Lys Gly Arg Val Val Tyr
1445 1450 1455
Phe Gly Pro Asn Gly Lys Leu Ala Leu Asn Tyr Phe His Thr Gln Cys
1460 1465 1470
Pro Cys Leu Ala Gly Leu Lys Glu Gly Glu Asn Glu Ala Glu Trp Leu
1475 1480 1485
Val Asp Leu Thr Thr Gln Ala Asp Arg Gln Gly Arg Ala Ala Asp Phe
1490 1495 1500
Ala Asp Thr Tyr Glu Arg Ser Glu Phe Lys Ala Ala Ala Glu Lys Glu
1505 1510 1515 1520
Ile Ala Ala Gln Leu Ala Thr Ala Ser Asp Leu Asp Glu Ala Thr Lys
1525 1530 1535
Arg Asp Leu Ala Val Arg Arg Asp Thr Val Thr Pro Phe Trp Trp Gly
1540 1545 1550
Leu Arg Thr Leu Leu Lys Tyr Arg Thr Leu Lys Asn Tyr Arg Asn Pro
1555 1560 1565
Glu Tyr Leu Gly Pro Arg Ile Gly Asp Lys Leu Ile Phe Ser Leu Leu
1570 1575 1580
Ile Phe Thr Leu Phe Trp Lys Val Gly Asn Asn Leu Ala Gln Asp Asn
1585 1590 1595 1600
Leu Met Asn Ile Ala Ala Val Leu Phe Met Trp Thr Thr Leu Pro Ala
1605 1610 1615
Phe Gly Ala Ala Ser Tyr Val Pro Ala Ile Val Leu Glu Arg Pro Leu
1620 1625 1630
Phe Thr Arg Glu Arg Asn Asp Gly Leu Tyr Arg Val Ile Thr Tyr Leu
1635 1640 1645
Val Ala Lys Ile Ile Glu Glu Leu Gly Leu Ala Leu Leu Cys Ser Ile
1650 1655 1660
Val Phe Ala Asn Ile Val Trp Trp Ala Leu Gln Leu Gln Gly Ser Phe
1665 1670 1675 1680
Ala Leu Phe Trp Leu Ile Tyr Phe Leu Thr Leu Ser Thr Gly Ile Val
1685 1690 1695
Leu Ala Tyr Thr Val Ala Ala Leu Ser Pro Asn Met Asp Val Ala Asn
1700 1705 1710
Ala Ala Leu Pro Ala Tyr Val Val Thr Leu Leu Phe Phe Ala Gly Phe
1715 1720 1725
Leu Ile Arg Phe Asp Lys Ile Pro Asn Tyr Trp Lys Trp Tyr Ser Tyr
1730 1735 1740
Ile Asp Val Leu Arg Tyr Ala Trp Gly Ala Leu Met Lys Asn Gln Phe
1745 1750 1755 1760
Asn Gly Asp Arg Asn Val Glu Phe Val Ser Gly Gln Thr Ile Leu Asp
1765 1770 1775
Tyr Tyr Ser Leu Ser Gly Ile Asn Ala Trp Gly Trp Leu Gly Ile Glu
1780 1785 1790
Ala Ala Phe Val Ala Val Phe Phe Gly Phe Ala Tyr Leu Ala Leu Lys
1795 1800 1805
Tyr Ile Ser His Pro Leu Lys Met Thr Thr Lys Asp Leu Glu Ala Gly
1810 1815 1820
Asp Ala Ser Thr Gly Ser Asp Gly Cys His Leu Gly Leu Glu Ala Ser
1825 1830 1835 1840
Pro Lys Lys Gly Leu Lys Gln Ser Leu Ser Arg Ala Phe Leu Ser Ser
1845 1850 1855
Gly Leu Gln Val Thr Phe Lys Asp Ile Thr Tyr Thr Val Val Asn Ser
1860 1865 1870
Gln Asn Lys Lys Glu Lys Leu Asp Leu Leu Lys Gly Val Gly Gly Tyr
1875 1880 1885
Leu Gln Ala Gly Glu Met Ala Ala Leu Met Gly Pro Ser Gly Ser Gly
1890 1895 1900
Lys Ser Thr Leu Leu Asp Val Leu Ser Gly Arg Lys Thr Val Gly Glu
1905 1910 1915 1920
Leu Gln Gly Glu Val Ala Phe Ala Gly Asn Arg Pro Thr Pro Gln Phe
1925 1930 1935
Leu Arg Arg Phe Thr Gly Tyr Val Glu Gln Phe Asp Thr Leu Leu Asp
1940 1945 1950
Ile Leu Thr Val Glu Glu Met Leu Met Tyr Thr Ala Glu Leu Lys Arg
1955 1960 1965
Pro Val Glu Gln Ser Leu Glu His Lys Arg Ala Ala Val Asp Glu Leu
1970 1975 1980
Ile Glu Val Leu Ala Leu Glu Gly Cys Arg Thr Val Arg Ile Gly Asn
1985 1990 1995 2000
Pro Met Ala Arg Gly Ile Ser Gly Gly Gln Ala Lys Arg Val Asn Ile
2005 2010 2015
Gly Ile Ala Leu Val Thr Asn Pro Arg Val Leu Phe Leu Asp Glu Pro
2020 2025 2030
Thr Ser Gly Leu Asp Ser Tyr Thr Ala Asn Glu Val Met Ser Val Val
2035 2040 2045
Lys Ser Leu Ala Ser His Gly Ile Thr Val Cys Ala Thr Ile His Ser
2050 2055 2060
Pro Thr Pro Tyr Thr Phe Asn Leu Phe Asp Arg Leu Leu Leu Leu Leu
2065 2070 2075 2080
Lys Gly Arg Val Val Tyr Phe Gly Pro Asn Gly Lys Leu Ala Leu Asp
2085 2090 2095
Tyr Phe His Thr Gln Cys Pro Cys Leu Ala Gly Leu Lys Glu Gly Glu
2100 2105 2110
Asn Glu Ala Glu Trp Leu Val Asp Leu Thr Thr Gln Ala Asp Arg Gln
2115 2120 2125
Gly Arg Ala Ala Asp Phe Ala Asp Thr Tyr Glu Arg Ser Glu Phe Lys
2130 2135 2140
Ala Ala Ala Asp Lys Glu Val Ala Ala Gln Leu Ala Thr Ala Ser Asn
2145 2150 2155 2160
Leu Asp Glu Ala Thr Lys Arg Asp Leu Ala Val Arg Arg Asp Thr Val
2165 2170 2175
Thr Pro Phe Trp Trp Gly Leu Arg Thr Leu Leu Lys Tyr Arg Thr Leu
2180 2185 2190
Lys Asn Tyr Arg Asn Pro Glu Tyr Leu Gly Pro Arg Ile Gly Asp Lys
2195 2200 2205
Leu Ile Phe Ser Leu Leu Ile Phe Thr Leu Phe Trp Lys Val Gly Asn
2210 2215 2220
Asn Leu Ala Gln Asp Asn Leu Met Asn Ile Thr Ser Met Leu Phe Met
2225 2230 2235 2240
Trp Thr Val Leu Pro Ala Phe Gly Ala Ala Ser Tyr Val Pro Ala Ile
2245 2250 2255
Val Leu Glu Arg Pro Leu Phe Thr Arg Glu Arg Asn Asp Gly Leu Tyr
2260 2265 2270
Arg Val Ile Thr Tyr Leu Val Ala Lys Ile Val Glu Glu Leu Gly Leu
2275 2280 2285
Ala Leu Leu Cys Ser Ile Val Phe Ala Asn Ile Val Trp Trp Ala Leu
2290 2295 2300
Gln Leu Gln Gly Ser Phe Ala Leu Phe Trp Leu Ile Tyr Tyr Leu Thr
2305 2310 2315 2320
Leu Ser Thr Gly Ile Val Leu Ala Tyr Thr Val Ala Ala Leu Ser Pro
2325 2330 2335
Asn Met Asp Val Ala Asn Ala Ala Leu Pro Ala Tyr Val Val Thr Leu
2340 2345 2350
Leu Phe Phe Ala Gly Cys Leu Ile Arg Phe Val Asp Ile Pro Asn Tyr
2355 2360 2365
Trp Lys Trp Tyr Ala Tyr Ile Asp Val Leu Arg Tyr Ala Trp Gly Ala
2370 2375 2380
Leu Met Lys Asn Gln Phe Asp Gly Asp Arg Asn Val Glu Phe Val Gly
2385 2390 2395 2400
Gly Gln Thr Ile Leu Asp Tyr Tyr Ser Leu Ser Gly Ile Asn Ala Trp
2405 2410 2415
Gly Trp Leu Gly Ile Glu Ala Ala Phe Val Ala Val Phe Phe Cys Phe
2420 2425 2430
Ala Tyr Leu Ala Leu Lys Tyr Ile Ser His Val Arg Arg
2435 2440 2445
<210> 70
<211> 282
<212> PRT
<213> 莱茵衣藻
<400> 70
Met Ala Thr Leu Lys Asp Asp Ala Asp Thr Lys Thr Val Leu Ala Glu
1 5 10 15
Ala Gly Leu Ile Ser Leu Ile Pro Gly His Trp Arg Asn Ala Asp Thr
20 25 30
Asn Leu Ser Leu Ser Arg Arg Tyr Gly Gly Gly Tyr Glu Val Val Tyr
35 40 45
Cys Ala Pro Pro Arg Ser Ser Gln Ala Gly Pro Val Ser Trp Ala Ala
50 55 60
Asn Lys Asp Pro Asn Ala Pro Ile Gln Ser Asn Pro Leu Gly Ser Phe
65 70 75 80
Ser Ser Gln Leu Gln Asn Gln Pro Thr Leu Pro Arg Ser Glu Glu Ala
85 90 95
Arg Lys Tyr Phe Arg Thr Val Tyr Asp Phe Pro Gln Trp Gln Thr His
100 105 110
Arg Asn Gln Tyr Arg Leu Met Lys Arg Leu Phe Ser Ile Pro Gln Ser
115 120 125
His Val Ile Gln Asn Ala Leu Pro Ser Ile Met Trp Val Ala Phe Thr
130 135 140
Ser Thr Cys Val Ala Ala Tyr Met Tyr Gly Tyr Asp Gln His Met Leu
145 150 155 160
Pro Glu Gly Phe Pro Thr Leu Ala Pro Asn Ala Ala Cys Ser Ala Phe
165 170 175
Ile Ser Asn Thr Ser Val Ala Leu Ser Leu Leu Leu Val Phe Arg Thr
180 185 190
Asn Ser Ser Tyr Gly Arg Trp Asp Glu Ala Arg Lys Met Trp Gly Gly
195 200 205
Leu Leu Asn Arg Ser Arg Asp Ile Met Arg Gln Gly Ala Thr Cys Phe
210 215 220
Pro Asp Asp Gln Val Glu Ala Lys Lys Ala Leu Ala Arg Trp Val Val
225 230 235 240
Ala Phe Ser Arg Ala Leu Arg Ile His Phe Gln Pro Glu Ser Gly Tyr
245 250 255
Trp Gln Ser Ala Arg Ala Leu Leu Leu Ala Gly Arg Ala Ser Val Ile
260 265 270
Gln Gly Ser Ala Cys Val Val Ala Thr Gln
275 280
<210> 71
<211> 302
<212> PRT
<213> 莱茵衣藻
<400> 71
Val Tyr Asp Phe Pro Gln Trp Gln Lys His Arg Ser Ser Tyr Arg Phe
1 5 10 15
Ala Glu Arg Leu Phe Gln Leu Ser Gln Ser His Ile Leu Gln Asn Ala
20 25 30
Leu Pro Ala Ile Ser Trp Val Thr Leu Val Ala Thr Leu Val Ala Ser
35 40 45
Tyr Gly Tyr Ser Tyr Asp Gln His Met Leu Pro Asp Val Phe Pro Ser
50 55 60
Ile Ser Pro Asn Ala Ser Cys Thr Ala Phe Ile Ser Asn Thr Ser Val
65 70 75 80
Ala Leu Ser Leu Leu Leu Val Phe Arg Thr Asn Ser Ser Tyr Gly Arg
85 90 95
Trp Asp Glu Ala Arg Lys Met Trp Gly Gly Leu Leu Asn Arg Ser Arg
100 105 110
Asp Ile Met Arg Gln Gly Ala Thr Cys Phe Pro Asp Asp Gln Val Glu
115 120 125
Ala Lys Lys Ala Leu Ala Arg Trp Thr Val Ala Phe Ser Arg Ala Leu
130 135 140
Arg Ile His Phe Gln Pro Glu Val Thr Ile Glu Ser Glu Leu Gln Asn
145 150 155 160
Ile Leu Thr Pro Ala Glu Leu Gln Met Leu Ala Lys Ser Gln His Arg
165 170 175
Pro Val Arg Ala Ile His Ala Ile Ser Gln Ile Ile Gln Ser Val Pro
180 185 190
Met Ser Ser Ile His Gln Gln Gln Met Ser Asn Asn Leu Thr Phe Phe
195 200 205
His Asp Val Leu Gly Gly Cys Glu Arg Leu Leu Arg Ala Pro Ile Pro
210 215 220
Val Ser Tyr Thr Arg His Thr Ala Arg Phe Leu Phe Ala Trp Leu Thr
225 230 235 240
Leu Leu Pro Phe Ala Leu Tyr Pro Thr Thr Gly Trp Gly Val Val Pro
245 250 255
Val Cys Thr Gly Ile Ala Ala Val Leu Cys Gly Ile Glu Glu Ile Gly
260 265 270
Val Gln Cys Glu Glu Pro Phe Gly Ile Leu Pro Leu Asp Val Ile Cys
275 280 285
Asn Arg Ile Gln Ala Asp Val Met Ala Thr Leu Lys Asp Asp
290 295 300
<210> 72
<211> 414
<212> PRT
<213> 莱茵衣藻
<400> 72
Met Ser Val Ala Arg Arg Ser Ala Ala Pro Glu Ile Phe Ile Leu Ala
1 5 10 15
Ala Ala Arg Ala Leu His Leu Cys Ser Leu Lys Lys Asn Ala Leu Ser
20 25 30
Asn Met Val Ile Arg Phe Met Ser Ile Asn Pro Arg Ser Leu Ser Trp
35 40 45
Leu Gln Ser Phe Cys Gln Lys Asn Ala Lys Ser Ala Ala Arg Asp Gln
50 55 60
Arg Gln Leu Tyr Glu Val Glu Gly Arg Gly Thr Val Pro Phe Asp His
65 70 75 80
Tyr Lys Glu Gly Ser Arg His Ala Arg Arg Met Thr Phe Thr Phe Asp
85 90 95
Asp Trp Lys Arg His Arg Ser Ser Asn Arg Tyr Leu Tyr His Leu Lys
100 105 110
Thr Leu Thr Glu Ser Gly Ile Val Arg Gly Ile Trp Ala Pro Val Ala
115 120 125
Trp Val Thr Leu Phe Thr Ala Val Val Ala Thr Leu Asn Val Ala His
130 135 140
Gly Ala Ala Met Leu Pro Pro Trp Val Pro Ala Met Pro Gln Ile Ala
145 150 155 160
Ile Glu Pro Val Gln Leu Thr Ser Ile Ala Leu Ser Leu Leu Leu Val
165 170 175
Phe Arg Thr Asn Ala Ser Tyr Ser Arg Trp Asp Glu Gly Arg Arg Ser
180 185 190
Phe Gly Ser Ile Thr Thr Val Ser Arg Asp Ile Ala Arg Gln Ala Phe
195 200 205
Ala Trp Phe Arg Pro Asp Asp Tyr Glu Ser Arg Val Arg Val Gly Arg
210 215 220
Trp Leu Val Ala Leu Gly Arg Ser Thr Met Val His Leu Arg Glu Glu
225 230 235 240
His Asp Met Glu Asp Glu Leu Arg Glu Val Leu Lys Pro Ala Glu Val
245 250 255
Gln Ala Val Val Ser Ala Val His Ala Pro Ser Phe Cys Leu Gln Met
260 265 270
Ile Thr Leu Ile Ile Arg Thr Ala Gly Leu Pro Gln Glu Leu Val Ile
275 280 285
Arg Met Asp Glu Asn Val Ser Arg Leu Thr Asp Ala Val Ser Ala Cys
290 295 300
Glu Arg Ile Leu Asn Thr Pro Ile Pro Leu Ser Tyr Thr Arg His Thr
305 310 315 320
Ala Arg Phe Leu Met Ala Trp Leu Ala Cys Leu Pro Phe Cys Leu Trp
325 330 335
Thr Tyr Cys Gly Pro Ala Met Val Pro Ile Ala Ala Leu Val Ala Phe
340 345 350
Val Leu Leu Gly Ile Glu Glu Ile Gly Val Tyr Ile Glu Glu Leu Asp
355 360 365
Arg Leu Val Lys Val Gly Ser Pro Thr Ala Ser Pro Arg Pro Glu Pro
370 375 380
Ala Ser Thr Asn Ser Asn Asn Ala Val Thr Asp Thr Ala Phe Gly Leu
385 390 395 400
Gly Ala Ala Gly Gly Phe Ser Pro Ala Val Ala Gly Ala Met
405 410
<210> 73
<211> 374
<212> PRT
<213> 三角褐指藻 CCAP 1055/1
<400> 73
Met Leu Ser Ser Ser Arg Trp Gly Ser Ala Phe Val Cys Phe Leu Ala
1 5 10 15
Leu Ala Cys Ser Ser Ser Tyr Arg Thr Gln Ala Phe Ile Val Pro Ser
20 25 30
Gly Thr Ser Ser Arg Val Ser Val Ala Val Arg Ser Leu Pro Pro Pro
35 40 45
Arg Asp Ile Ala Tyr Gly Glu Glu Ser Arg Lys Tyr Arg Arg Thr Val
50 55 60
Tyr Thr His Asp Asp Trp Val Asn His Arg Ser Pro Asp Arg Phe Trp
65 70 75 80
Arg Asn Ile Ile Ala Met Pro Thr Ser Gly Val Tyr Lys Asn Leu Ala
85 90 95
Lys Glu Cys Ile Ala Thr Thr Ala Val Ala Thr Ala Ile Val Val Tyr
100 105 110
Asn Ala Leu Val Gly Gly Tyr Thr Asp Phe Gly Gly Val Gln His Ala
115 120 125
Ala Val Leu Gln Asn Glu Leu Leu Pro Lys Ile Gly Met Pro Val Ser
130 135 140
Pro Phe Thr Val Ser Gly Ser Phe Leu Gly Phe Leu Leu Ile Phe Arg
145 150 155 160
Thr Asn Ser Ser Tyr Lys Arg Trp Asp Glu Ala Arg Lys Asn Trp Gly
165 170 175
Met Asn Ile Asn His Thr Arg Asp Leu Val Arg Met Gly Thr Ala Phe
180 185 190
Tyr Asp Lys Thr Gly Val Thr Asp Glu Gln Arg Lys Lys Asp Leu Gln
195 200 205
Ala Leu Ser Leu Ala Thr Trp Ser Phe Val Arg Ala Met Lys Arg His
210 215 220
Leu Ser Pro Glu Gln Glu Asp Glu Gln Asp Phe Arg Arg Glu Leu His
225 230 235 240
Glu Arg Leu Pro Pro Arg Gln Ala Gln Ala Ile Ile Asp Ala Ala His
245 250 255
Arg Pro Asn Arg Ala Leu Phe Asp Leu Ser Val Ala Ile Glu Asn Leu
260 265 270
Pro Met His Phe Met Arg Lys Asn Glu Ile His Asn Ala Ala Thr Ile
275 280 285
Phe Glu Asp Asn Leu Gly Ser Ser Glu Arg Leu Leu Thr Ser Pro Ile
290 295 300
Pro Leu Phe Tyr Ala Arg His Thr Ala Arg Phe Leu Gly Val Trp Leu
305 310 315 320
Leu Leu Met Pro Phe Cys Leu Tyr Asp Pro Phe Ala Gly Ser Trp Asn
325 330 335
His Val Gly Met Ile Pro Ala Thr Ala Leu Ile Ser Ile Phe Leu Phe
340 345 350
Gly Ile Glu Glu Leu Ala Thr Ser Met Glu Glu Pro Phe Thr Ile Leu
355 360 365
Pro Met Gln Ala Phe Cys
370
<210> 74
<211> 455
<212> PRT
<213> 三角褐指藻 CCAP 1055/1
<400> 74
Met Met Arg Asn Phe Ala Ser Val Leu Leu Leu Leu Ser Ser Gly Ala
1 5 10 15
Ala Ala Phe Ala Pro Val Gln His Asn Gly Val Arg Thr Ile Ala Thr
20 25 30
Pro Ser Thr Pro Leu Tyr Gly Asn Thr Lys Gln Pro Pro Ala Leu Pro
35 40 45
Pro Ile Lys Asp Ile Ser Tyr Gly Glu Glu Ser Arg Lys Tyr Arg Arg
50 55 60
Thr Val Tyr Ser His Asp Asp Trp Val Lys His Arg Ser Ser Asp Arg
65 70 75 80
Phe Leu Arg Asn Leu Leu Ala Ile Gly Ser Ser Gly Val Tyr Lys Ser
85 90 95
Leu Ala Lys Glu Val Leu Ala Thr Thr Gly Val Ala Thr Phe Ile Val
100 105 110
Leu Tyr Asn Cys Leu Val Gly Gly Tyr Thr Asp Leu Glu Gly Ile Lys
115 120 125
His Ser Ala Leu Ile Glu Ser Val Trp Ala Pro Leu Met Ala Leu Pro
130 135 140
Leu Ala Pro Phe Thr Leu Ser Ser Pro Ser Leu Gly Leu Leu Leu Val
145 150 155 160
Phe Arg Thr Asn Thr Ser Tyr Gln Arg Trp Asp Glu Ala Arg Lys Asn
165 170 175
Trp Gly Met Asn Ile Asn His Thr Arg Asp Leu Val Arg Met Gly Thr
180 185 190
Ser Phe Tyr Asp Asn Ala Ala Val Ser Ser Glu Gln Arg Ala Lys Asp
195 200 205
Leu Lys Ala Leu Ser Leu Ala Thr Trp Ser Phe Val Arg Ala Met Lys
210 215 220
Arg His Leu Ser Pro Glu Ser Glu Asp Glu Gln Asp Phe Arg Arg Glu
225 230 235 240
Leu Phe Glu Arg Leu Pro Ala Pro Gln Ala Gln Ala Ile Ile Asp Ala
245 250 255
Ala His Arg Pro Asn Arg Ala Leu Phe Asp Leu Ser Val Ala Ile Glu
260 265 270
Asn Leu Pro Met His Phe Leu Arg Lys Asn Gln Val His Gln Ala Val
275 280 285
Thr Ile Phe Glu Asp Asn Leu Gly Ser Ser Glu Arg Leu Leu Thr Ser
290 295 300
Pro Val Pro Leu Phe Tyr Ser Arg His Thr Ala Arg Phe Leu Ser Phe
305 310 315 320
Trp Leu Leu Leu Leu Pro Phe Ala Leu Trp Asp Pro Phe Ala Gly Thr
325 330 335
Trp Asn His Val Gly Met Ile Pro Ala Thr Ala Val Ile Ser Ile Phe
340 345 350
Leu Phe Gly Ile Glu Glu Leu Ala Thr Gln Met Glu Glu Pro Phe Thr
355 360 365
Ile Leu Pro Met Gln Ala Phe Cys Asp Lys Ile Gly Asn Trp Cys Asn
370 375 380
Glu Ile Val Ser Trp Gln Ala Gly Asp Asn Gly Met Ala Val Asn Met
385 390 395 400
Pro Ser Met Ile Ser Pro Glu Gly Leu Pro Glu Leu Lys Glu Pro Ala
405 410 415
Pro Val Pro Ala Met Ala Val Ala Ser Val Ala Ala Ala Met Pro Val
420 425 430
Met Ala Asn Gly Asp Ile Asn Gly Asp Thr Thr Gly Ile Thr Met Asp
435 440 445
Gln Pro His Asn Ala Ile Pro
450 455
<210> 75
<211> 408
<212> PRT
<213> 假微型海链藻 CCMP1335
<400> 75
Met Pro Ser Phe Thr Ser Leu Ser Thr Leu Leu Leu Leu Ala Leu Ser
1 5 10 15
Ser Pro Gln Ile Ser Ala Phe Ala Pro Leu Ser Ser Thr Ser Thr Pro
20 25 30
Ile Asn Val Ala Pro Ser Thr Thr Thr Ser Thr Thr Asn Leu Gln Met
35 40 45
Gly Pro Pro Lys Thr Asp Ile Val Leu Ser Glu Thr Tyr Gly Glu Gly
50 55 60
Ser Arg Lys Tyr Arg Arg Thr Val Tyr Thr His Asn Glu Trp Val Lys
65 70 75 80
His Arg Ser Ser Asp Arg Phe Ala Lys Asn Leu Phe Ser Met Val Asn
85 90 95
Ser Gly Val Tyr Lys Ser Leu Ala Lys Glu Val Phe Ala Thr Thr Ala
100 105 110
Val Ala Ser Ala Ile Val Ala Trp Asn Gly Ile Ala Gly Gly Tyr Thr
115 120 125
Asp Phe Asn Gly Val Glu His Gly Ala Ile Met Ser Phe Leu Pro Gln
130 135 140
Leu Val Leu Pro Leu Thr Pro Phe Thr Leu Leu Ser Pro Ser Leu Gly
145 150 155 160
Leu Leu Leu Val Phe Arg Thr Asn Ser Ser Tyr Gly Arg Trp Asp Glu
165 170 175
Ala Arg Lys Met Trp Gly Leu Asn Ile Asn His Thr Arg Asp Leu Asn
180 185 190
Arg Met Ala Thr Ala Trp Tyr Gly His Asp Asn Gln Ile Ile Asp Pro
195 200 205
Ala Lys Arg Ala Glu Asp Leu Arg Gln Val Ser Leu Tyr Thr Trp Ala
210 215 220
Phe Val Arg Ser Met Lys Arg His Leu Ser Pro Pro Ser Glu Asp Glu
225 230 235 240
Glu Ala Phe Val Glu Glu Leu Tyr Ala Arg Met Ala Pro Glu Gln Ala
245 250 255
Glu Ala Ile Ile Ser Ala Ala His Arg Pro Asn Arg Ala Leu Tyr Asp
260 265 270
Leu Ser Val Val Ile Asp Lys Leu Pro Met His Phe Met Arg Lys Asn
275 280 285
Glu Ile Asn Lys Asn Leu Ser Ile Phe Glu Asp Thr Leu Gly Gly Cys
290 295 300
Glu Arg Leu Leu Ser Ser Pro Val Pro Leu Phe Tyr Thr Arg His Thr
305 310 315 320
Ala Arg Phe Leu Ser Thr Trp Leu Leu Leu Leu Pro Leu Ala Met Tyr
325 330 335
Gln Pro Phe Ser Gly Ser Trp Asn His Val Ala Met Ile Pro Ala Thr
340 345 350
Ala Leu Thr Ser Val Phe Leu Phe Gly Ile Asp Glu Leu Ser Thr Gln
355 360 365
Leu Glu Glu Pro Phe Thr Ile Leu Pro Met Gln Gly Phe Cys Asp Lys
370 375 380
Ile Gly Gly Trp Cys Asp Glu Ile Val Ser Trp Arg Gly Gln Gly Leu
385 390 395 400
Asp Lys Glu Glu Gln Gln Tyr Tyr
405
<210> 76
<211> 390
<212> PRT
<213> 假微型海链藻 CCMP1335
<400> 76
Met Gly Pro Pro Ile Asp Pro Ser Val Pro Val Thr Asp Gln Val Gly
1 5 10 15
Glu Gly Ser Arg Lys Tyr Arg Arg Thr Val Tyr Thr His Asp Asp Trp
20 25 30
Val Arg His Arg Ser Pro Asp Arg Phe Gly Asn Asn Leu Ser Thr Leu
35 40 45
Phe Asn Ser Gly Ile Tyr Lys Gln Val Ala Asn Glu Val Phe Ala Thr
50 55 60
Thr Ala Val Ala Thr Phe Val Phe Leu Trp Asn Met Ile Ala Gly Gly
65 70 75 80
Tyr Thr Asp Leu Ala Gly Val Gln His Gly Pro Ile Ile Asp Ser Pro
85 90 95
Leu Ala Gln Met Val Gly Leu Pro Met Thr Ala Phe Thr Ile Leu Thr
100 105 110
Pro Ser Leu Gly Leu Leu Leu Val Phe Arg Thr Asn Thr Ser Tyr Gly
115 120 125
Arg Trp Asp Glu Ala Arg Lys Met Trp Gly Leu Asn Ile Asn His Thr
130 135 140
Arg Asp Leu Asn Arg Met Ala Thr Ala Trp Tyr Gly Asn Glu Gly Asn
145 150 155 160
Met Asp Ser Val Ala Phe Met Gly Gly Asp Ile Pro Tyr Ser Gln Pro
165 170 175
Ile Asp Pro Val Gln Arg Ala Tyr Asp Leu Gly Gln Val Ser Leu Phe
180 185 190
Thr Trp Ala Phe Val Arg Ser Met Lys Arg His Leu Ser Pro Pro Glu
195 200 205
Glu Asp Glu Glu Asp Phe Lys Ala Glu Leu Arg Ala Arg Leu Thr Pro
210 215 220
Glu Gln Ala Glu Asn Ile Ile Asn Ala Ala His Arg Pro Asn Arg Ala
225 230 235 240
Leu Phe Asp Leu Ser Val Ala Ile Glu Asn Leu Pro Met His Phe Leu
245 250 255
Arg Lys Asn Ala Ile Asn Thr Asn Leu Ser Ile Phe Glu Asp Thr Leu
260 265 270
Gly Gly Cys Glu Arg Leu Leu Ser Ser Pro Val Pro Leu Phe Tyr Ser
275 280 285
Arg His Thr Ala Arg Phe Leu Ser Thr Trp Leu Leu Leu Leu Pro Phe
290 295 300
Gly Leu Tyr Glu Gln Phe Lys Asp Ser Trp Asn His Ile Ala Met Ile
305 310 315 320
Pro Ala Thr Ala Phe Ile Ser Val Cys Leu Phe Gly Ile Glu Glu Leu
325 330 335
Ala Thr Gln Leu Glu Glu Pro Phe Thr Ile Leu Pro Met Gln Gly Phe
340 345 350
Cys Asp Lys Ile Gly Gly Trp Cys Asp Glu Ile Val Ser Trp Ala Gly
355 360 365
Gln Gly Gln Gln Glu Tyr Thr Glu Glu Asn Ala Met Ser Asn Glu Gln
370 375 380
Glu Met Thr Tyr Trp Arg
385 390
<210> 77
<211> 426
<212> PRT
<213> 黄色猴面花
<400> 77
Met Thr Asn Pro Ile His His Ser Ala Thr Lys Leu Ser Pro Ser Ser
1 5 10 15
Ser Ser Ser Ser Leu Ser Ser His Phe Thr Pro Lys Thr Leu Lys Lys
20 25 30
Phe His Leu Tyr Pro Ser Asn Asn His His Arg Ser Asn His His Leu
35 40 45
Arg Phe Ser Cys Cys Gln Ser Ser Lys Asp Ser Asn Phe Ser Leu Asn
50 55 60
Gln Asp Ser Thr Phe Phe Ser Ile Leu Leu Ala Leu Pro Asn Trp Val
65 70 75 80
Asp Ser Ile Gln Glu Lys Gly Leu Glu Lys Lys Arg Ser Leu Tyr Asp
85 90 95
His Gly Asp Trp Val Gln His Arg Ser Ser Ser Arg His Val Arg His
100 105 110
Phe Leu Ser Ser Leu Gly Ser Arg Val Ile Leu Ser Leu Leu Pro Pro
115 120 125
Val Ile Val Phe Thr Ser Val Ala Val Val Val Ser Thr Tyr Asn Thr
130 135 140
Ala Val Ala Leu Ala Trp Leu Pro Glu Phe Phe Pro Ile Leu Arg Ala
145 150 155 160
Ser Ser Leu Pro Tyr Gln Leu Thr Ala Pro Ala Leu Ala Leu Leu Leu
165 170 175
Val Phe Arg Thr Glu Ala Ser Tyr Ser Arg Phe Asp Glu Gly Lys Lys
180 185 190
Ala Trp Thr Lys Val Val Ser Gly Thr Asn Asp Phe Ala Arg Gln Val
195 200 205
Ile Ala Ser Ala Val Gly Pro Asp Asp Ser Val Leu Lys Thr Ala Ile
210 215 220
Leu Gln Tyr Val Val Ala Phe Pro Val Ala Leu Lys Cys His Val Ile
225 230 235 240
His Asp Ser Asp Ile Glu Gln Asp Leu Lys Asn Ile Leu Glu Thr Asp
245 250 255
Asp Leu Ala Val Val Leu Thr Ser Asn His Arg Pro Arg Cys Ile Ile
260 265 270
Glu Phe Ile Ser Gln Ser Leu Arg Ser Leu Asp Val Asp Pro Thr Lys
275 280 285
Leu Gln Val Leu Glu Ser Lys Leu Ser Cys Phe His Glu Gly Ile Gly
290 295 300
Val Cys Glu Gln Leu Leu Gly Ile Pro Ile Pro Leu Ser Tyr Thr Arg
305 310 315 320
Leu Thr Ser Arg Phe Leu Val Leu Trp His Phe Thr Leu Pro Ile Ile
325 330 335
Leu Trp Asp Asp Cys His Trp Ile Val Val Pro Ala Thr Phe Ile Ser
340 345 350
Ala Ala Ser Leu Phe Cys Ile Glu Glu Val Gly Val Leu Ile Glu Glu
355 360 365
Pro Phe Pro Met Leu Ala Leu Asp Glu Leu Cys Glu Lys Val Arg Ser
370 375 380
Asn Ile Glu Asp Val Met Arg Asn Glu Lys Leu Ile Gln Ala Arg Val
385 390 395 400
Asn Ala Lys Arg Lys Thr His Tyr Lys Arg Asp Ser Pro Asn Gly Trp
405 410 415
Thr Ala Ser Leu Glu Asn Arg Lys Pro Asp
420 425
<210> 78
<211> 412
<212> PRT
<213> 番茄
<400> 78
Met Thr Asn Pro Arg Thr Leu Phe Thr Ile Gln Ser Pro Thr Asn Ala
1 5 10 15
Ser Phe Ser Ser His Phe Thr Leu Lys Asn Pro Ser Lys Ser Gln Gln
20 25 30
Gln Pro Pro Val Tyr Lys Lys Leu Asn Phe Arg Val Leu Cys Ser Ser
35 40 45
Gln Pro Ser Arg Thr Pro Gln Asn Ser Asn Leu Ile Ser Thr Leu Val
50 55 60
Lys Ile Leu Arg Val Val Pro Asp Trp Ala Asp Lys Ile Gln Glu Gly
65 70 75 80
Gly Met Arg Lys Lys Arg Ser Leu Tyr Lys His Glu Thr Trp Val Gln
85 90 95
His Arg Ser Ser Leu Arg His Val Arg His Leu Phe Ser Ser Phe Asn
100 105 110
Ser Arg Val Val Leu Ser Leu Ile Pro Pro Val Ile Ala Phe Thr Ser
115 120 125
Phe Ala Phe Val Ile Ala Ser Tyr Asn Ser Ala Val Ser Phe His Trp
130 135 140
Leu Pro Glu Phe Phe Pro Ile Leu Arg Ala Ser Pro Gln Pro Tyr Gln
145 150 155 160
Leu Thr Ala Pro Ala Leu Ala Leu Leu Leu Val Phe Arg Thr Glu Ala
165 170 175
Ser Tyr Ser Arg Phe Glu Ala Gly Lys Lys Ala Trp Thr Lys Val Ile
180 185 190
Ala Gly Thr Asn Asp Phe Ala Arg Gln Val Ile Ala Cys Val Asp Lys
195 200 205
Arg Asp Asp Val Leu Lys Glu Ala Leu Leu Gln Tyr Ile Met Ala Phe
210 215 220
Pro Val Ala Leu Lys Cys His Ile Val Tyr Asp Ser Asp Ile Ala Ser
225 230 235 240
Asp Leu Lys Asn Leu Leu Glu Ala Asp Asp Leu Ala Val Val Leu Ser
245 250 255
Ser Lys His Arg Pro Arg Cys Ile Ile Gly Phe Ile Ala Gln Ser Leu
260 265 270
Gln Ser Leu Asn Leu Glu Gly Thr Ile Leu Ser Gln Leu Glu Ser Lys
275 280 285
Ile Ser Cys Phe His Glu Gly Ile Gly Val Cys Glu Gln Leu Leu Gly
290 295 300
Ile Pro Ile Pro Leu Ser Tyr Thr Arg Leu Thr Ser Arg Phe Leu Val
305 310 315 320
Leu Trp His Leu Thr Leu Pro Ile Ile Leu Trp Asp Asp Cys His Trp
325 330 335
Ile Val Val Pro Ala Thr Phe Ile Ser Ala Ala Ser Leu Phe Cys Ile
340 345 350
Glu Glu Val Gly Val Leu Ile Glu Glu Pro Phe Pro Met Leu Ala Leu
355 360 365
Asp Glu Leu Cys Gln Arg Val His Asp Asn Ile His Glu Ala Met Ala
370 375 380
Asn Glu Lys Gln Ile Gln Glu Ile Val Asn Ser Lys Arg Lys Arg Ser
385 390 395 400
Phe Ser Glu His Ser Pro Asn Gly Trp Pro Thr Ser
405 410
<210> 79
<211> 414
<212> PRT
<213> 野草莓亚属
<400> 79
Met Leu Thr Gln Pro Thr Asn Cys Ile Lys Pro Thr Ser Asn Ile Leu
1 5 10 15
Phe Ser Phe Asn Ser Ile Pro Lys Ser Phe Phe Lys Leu His Pro Pro
20 25 30
Leu Asn Pro Arg Thr Leu Ser Leu Lys His His Cys Ser Gln Tyr Pro
35 40 45
Asn Pro Thr Pro Ser Ser Ser Ser Ser Ser Ser Pro Phe Gln Pro Leu
50 55 60
Ser Ser Ile Leu Arg Ile Ile Pro Asp Trp Ala Asp Glu Ile Lys Glu
65 70 75 80
Arg Gly Phe Arg Gln Asp Arg Thr Leu Tyr Asp His Glu Lys Trp Val
85 90 95
His His Arg Ser Ser Tyr Arg His Leu Arg His Phe Leu Thr Ser Leu
100 105 110
Ser Ser Arg Val Ile Leu Ser Leu Ile Pro Pro Val Ile Ala Phe Thr
115 120 125
Leu Val Ala Val Val Ile Ala Ser Tyr Asn Thr Ala Val Ala Leu Glu
130 135 140
Trp Leu Pro Glu Val Phe Pro Met Leu Arg Ser Ser Thr Leu Pro Tyr
145 150 155 160
Gln Leu Thr Ala Pro Ala Leu Ala Leu Leu Leu Val Phe Arg Thr Glu
165 170 175
Ala Ser Tyr Ser Arg Phe Glu Glu Gly Arg Lys Ala Trp Thr Lys Val
180 185 190
Ile Ala Gly Thr Asn Asp Phe Ala Arg Gln Ile Ile Ser Gly Val Glu
195 200 205
Asn Ser Gly Asp Ala Glu Leu Lys Lys Ala Leu Leu Gln Tyr Val Val
210 215 220
Ala Phe Pro Val Ala Leu Lys Cys His Val Ile Tyr Gly Ser Asn Met
225 230 235 240
Gly Arg Asp Leu Gln Asn Leu Leu Glu Val Asp Asp Leu Leu Val Val
245 250 255
Leu Ser Ser Lys His Gly Pro Gly Cys Ile Ile Glu Phe Ile Ser Gln
260 265 270
Asn Ile Arg Met Leu Lys Leu Glu Glu Ser Arg Arg Asn Met Leu Glu
275 280 285
Ser Asn Met Ser Cys Phe His Glu Gly Ile Ser Val Cys Glu Asn Leu
290 295 300
Ile Gly Thr Pro Ile Pro Leu Ser Tyr Thr Arg Leu Thr Ser Arg Phe
305 310 315 320
Leu Val Leu Trp His Leu Thr Leu Pro Ile Ile Leu Trp Asp Asp Cys
325 330 335
His Trp Ile Val Val Pro Ala Thr Phe Ile Ser Ala Ala Ser Leu Phe
340 345 350
Cys Ile Glu Glu Val Gly Val Leu Ile Glu Glu Pro Phe Pro Met Leu
355 360 365
Ala Leu Asp Asp Leu Cys Arg Ser Val Gln Glu Asn Val Gln Glu Ala
370 375 380
Leu Ser Arg Glu Lys Leu Ile Gln Ala Arg Leu Thr Ala Lys Gly Arg
385 390 395 400
Ile Gln Lys Gln Gln His Cys Pro Asn Gly Gln Pro Lys Ser
405 410
<210> 80
<211> 416
<212> PRT
<213> 木本棉
<400> 80
Met Ala Gln Ser Pro Asn Ser Pro Lys Leu Ser Leu Ser Pro Ser Phe
1 5 10 15
Thr Ser Lys Pro Phe Leu His Phe His Phe Asn Ser Ser Leu Pro Ala
20 25 30
Lys Phe Lys Arg Pro Pro Ser Phe Lys Ile Leu Phe Ser Ala Ser Gln
35 40 45
Pro Pro Pro Pro Ser Ser Lys Pro Thr Thr Ala Pro Lys Thr Phe Asn
50 55 60
Leu Ile Thr Leu Leu Arg Ala Ile Pro Asp Trp Ala Asp Arg Ile Gln
65 70 75 80
Glu Arg Gly Met Gln Gln Asn Arg Ala Leu Tyr Asn His Glu Lys Trp
85 90 95
Val His His Arg Ser Ser Leu Arg His Leu Arg His Leu Leu Ser Ser
100 105 110
Leu Gln Ser Arg Val Ile Leu Ser Leu Val Pro Pro Val Leu Ala Phe
115 120 125
Thr Ser Val Ala Thr Val Val Ala Leu Tyr Asn Thr Ala Val Asp Leu
130 135 140
His Trp Phe Pro Gly Phe Phe Pro Val Leu Arg Ala Ser Ser Leu Pro
145 150 155 160
Tyr Gln Leu Thr Ala Pro Ala Leu Ala Leu Leu Leu Val Phe Arg Thr
165 170 175
Glu Ala Ser Tyr Ser Arg Tyr Glu Glu Gly Arg Lys Ala Trp Thr Lys
180 185 190
Val Ile Ala Gly Thr Asn Asp Phe Ala Arg Gln Val Ile Ala Gly Val
195 200 205
Asp Asn Ser Ala Asp Asp Gln Ser Ile Lys Ala Ala Leu Leu Gln Tyr
210 215 220
Ile Met Ala Phe Pro Val Ala Leu Lys Cys His Val Met Tyr Gly Ser
225 230 235 240
Asp Ile Gly Arg Asp Leu Gln Asn Leu Leu Glu Val Asp Asp Leu Ala
245 250 255
Val Ile Leu Asn Ser Lys His Arg Pro Arg Cys Ile Ile Glu Phe Ile
260 265 270
Ser Gln Ser Leu His Leu Leu Asn Leu Glu Glu Ser Lys Arg Thr Thr
275 280 285
Leu Glu Ser Lys Ile Ser Cys Phe His Glu Gly Ile Gly Val Cys Glu
290 295 300
Gln Leu Met Gly Ile Pro Ile Pro Leu Ser Tyr Thr Arg Leu Thr Ser
305 310 315 320
Arg Phe Leu Val Leu Trp His Leu Thr Leu Pro Ile Ile Leu Trp Asp
325 330 335
Asp Cys His Trp Ile Val Val Pro Ala Thr Phe Ile Ser Ala Ala Ser
340 345 350
Leu Phe Cys Ile Glu Glu Val Gly Val Leu Ile Glu Glu Pro Phe Pro
355 360 365
Met Leu Ala Leu Asp Glu Leu Cys Ile Val Thr Gln Asn Asn Ile Arg
370 375 380
Glu Ala Ile Ala Thr Glu Lys Asp Ile Gln Ala Met Leu Lys Gly Lys
385 390 395 400
Arg Lys Arg His Ser Tyr Lys Arg Ser Pro Asn Gly Cys Pro Asn Thr
405 410 415
<210> 81
<211> 416
<212> PRT
<213> 陆地棉
<400> 81
Met Ala Gln Ser Pro Asn Ser Pro Lys Leu Ser Leu Ser Ser Ser Phe
1 5 10 15
Thr Ser Lys Pro Phe Leu His Phe His Ser Asn Ser Ser Leu Pro Ala
20 25 30
Lys Phe Lys Arg Pro Pro Ser Phe Lys Ile Leu Phe Ser Ala Ser Gln
35 40 45
Pro Pro Pro Pro Ser Ser Lys Pro Thr Thr Ala Pro Lys Thr Phe Asn
50 55 60
Leu Ile Thr Leu Leu Arg Ala Ile Pro Asp Trp Ala Asp Arg Ile Gln
65 70 75 80
Glu Arg Gly Met Gln Gln Asn Arg Ala Leu Tyr Asn His Glu Lys Trp
85 90 95
Val His His Arg Ser Ser Leu Arg His Leu Arg His Leu Leu Ser Ser
100 105 110
Leu Gln Ser Arg Val Ile Leu Ser Leu Val Pro Pro Val Leu Ala Phe
115 120 125
Thr Ser Val Ala Ile Val Ile Ala Leu Tyr Asn Thr Ala Val Asp Leu
130 135 140
His Trp Phe Pro Gly Phe Phe Pro Val Leu Arg Ala Ser Ser Leu Pro
145 150 155 160
Tyr Gln Leu Thr Ala Pro Ala Leu Ala Leu Leu Leu Val Phe Arg Thr
165 170 175
Glu Ala Ser Tyr Ser Arg Tyr Glu Glu Gly Arg Lys Ala Trp Thr Lys
180 185 190
Val Ile Ala Gly Thr Asn Asp Phe Ala Arg Gln Val Ile Ala Gly Val
195 200 205
Asp Asn Ser Ala Asp Asp Gln Ser Ile Lys Ala Ala Leu Leu Gln Tyr
210 215 220
Ile Met Ala Phe Pro Val Ala Leu Lys Cys His Val Met Tyr Gly Ser
225 230 235 240
Asp Ile Gly Arg Asp Leu Gln Asn Leu Leu Glu Val Asp Asp Leu Ala
245 250 255
Leu Ile Leu Asn Ser Lys His Arg Pro Arg Cys Ile Ile Glu Phe Ile
260 265 270
Ser Gln Ser Leu Gln Leu Leu Asn Leu Glu Glu Ser Lys Arg Thr Met
275 280 285
Leu Glu Ser Lys Ile Ser Cys Phe His Glu Gly Ile Gly Val Cys Glu
290 295 300
Gln Leu Met Gly Ile Pro Ile Pro Leu Ser Tyr Thr Arg Leu Thr Ser
305 310 315 320
Arg Phe Leu Val Leu Trp His Leu Thr Leu Pro Ile Ile Leu Trp Asp
325 330 335
Asp Cys His Trp Ile Val Val Pro Ala Thr Phe Ile Ser Ala Ala Ser
340 345 350
Leu Phe Cys Ile Glu Glu Val Gly Val Leu Ile Glu Glu Pro Phe Pro
355 360 365
Met Leu Ala Leu Asp Glu Leu Cys Ile Val Thr Gln Asn Asn Ile Lys
370 375 380
Glu Ala Ile Ala Thr Glu Lys Asp Ile Gln Ala Met Leu Lys Gly Lys
385 390 395 400
Arg Lys Arg His Ser Tyr Lys His Ser Pro Asn Gly Cys Pro Asn Thr
405 410 415
<210> 82
<211> 416
<212> PRT
<213> 雷蒙德氏棉
<400> 82
Met Ala Gln Ser Pro Asn Ser Pro Lys Leu Ser Leu Ser Ser Ser Phe
1 5 10 15
Thr Ser Lys Pro Phe Leu His Phe His Ser Asn Ser Ser Leu Pro Ala
20 25 30
Lys Phe Lys Arg Pro Pro Ser Phe Lys Ile Leu Phe Ser Ala Ser Gln
35 40 45
Pro Pro Pro Pro Ser Ser Lys Pro Thr Thr Ala Pro Lys Thr Phe Asn
50 55 60
Leu Ile Thr Leu Leu Arg Ala Ile Pro Asp Trp Ala Asp Arg Ile Gln
65 70 75 80
Glu Arg Gly Met Gln Gln Asn Arg Ala Leu Tyr Asn His Glu Lys Trp
85 90 95
Val His His Arg Ser Ser Leu Arg His Leu Arg His Leu Leu Ser Ser
100 105 110
Leu Gln Ser Arg Val Ile Leu Ser Leu Val Pro Pro Val Leu Ala Phe
115 120 125
Thr Ser Val Ala Ile Val Val Ala Leu Tyr Asn Thr Ala Val Asp Leu
130 135 140
His Trp Phe Pro Gly Phe Phe Pro Val Leu Arg Ala Ser Ser Leu Pro
145 150 155 160
Tyr Gln Leu Thr Ala Pro Ala Leu Ala Leu Leu Leu Val Phe Arg Thr
165 170 175
Glu Ala Ser Tyr Ser Arg Tyr Glu Glu Gly Arg Lys Ala Trp Thr Lys
180 185 190
Val Ile Ala Gly Thr Asn Asp Phe Ala Arg Gln Val Ile Ala Gly Val
195 200 205
Asp Asn Ser Ala Asp Asp Gln Ser Ile Lys Ala Ala Leu Leu Gln Tyr
210 215 220
Ile Met Ala Phe Pro Val Ala Leu Lys Cys His Val Met Tyr Gly Ser
225 230 235 240
Asp Ile Gly Arg Asp Leu Gln Asn Leu Leu Glu Val Asp Asp Leu Ala
245 250 255
Leu Ile Leu Asn Ser Lys His Arg Pro Arg Cys Ile Ile Glu Phe Ile
260 265 270
Ser Gln Ser Leu Gln Leu Leu Asn Leu Glu Glu Ser Lys Arg Thr Met
275 280 285
Leu Glu Ser Lys Ile Ser Cys Phe His Glu Gly Ile Gly Val Cys Glu
290 295 300
Gln Leu Met Gly Ile Pro Ile Pro Leu Ser Tyr Thr Arg Leu Thr Ser
305 310 315 320
Arg Phe Leu Val Leu Trp His Leu Thr Leu Pro Ile Ile Leu Trp Asp
325 330 335
Asp Cys His Trp Ile Val Val Pro Ala Thr Phe Ile Ser Ala Ala Ser
340 345 350
Leu Phe Cys Ile Glu Glu Val Gly Val Leu Ile Glu Glu Pro Phe Pro
355 360 365
Met Leu Ala Leu Asp Glu Leu Cys Ile Val Thr Gln Asn Asn Ile Lys
370 375 380
Glu Ala Ile Ala Thr Glu Lys Asp Ile Gln Ala Met Leu Lys Gly Lys
385 390 395 400
Arg Lys Arg His Ser Tyr Lys His Ser Pro Asn Gly Cys Pro Asn Thr
405 410 415
<210> 83
<211> 417
<212> PRT
<213> 烟草植物
<400> 83
Met Thr Asn Ser Arg Thr Leu Phe Ser Ile Gln Ser Pro Thr Asn Ala
1 5 10 15
Ser Phe Ser Ser His Phe Thr Leu Lys Leu Pro Ser Lys Leu Gln Gln
20 25 30
Gln Ser Phe Pro Ser Lys Leu Ser Cys Lys Lys Phe Ser Phe Thr Thr
35 40 45
Phe Lys Val Arg Cys Cys Pro Gln Gln Thr Pro Gln Asn Gln Asn Ser
50 55 60
Thr Ser Ala Leu Ile Ser Ile Leu Arg Ile Ile Pro Asp Trp Ala Asp
65 70 75 80
Arg Ile Gln Glu Gly Gly Met Lys Lys Lys Arg Ser Leu Tyr Lys His
85 90 95
Glu Ser Trp Val Gln His Arg Ser Ser Leu Arg His Val Arg His Leu
100 105 110
Phe Ser Ser Leu Asn Ser Arg Val Ile Leu Ser Leu Val Pro Pro Val
115 120 125
Ile Ala Phe Thr Ser Val Ala Val Val Ile Ala Ser Tyr Asn Ser Ala
130 135 140
Val Leu Met His Trp Leu Pro Glu Phe Phe Pro Val Leu Arg Ala Ser
145 150 155 160
Pro Leu Pro Tyr Gln Leu Thr Ala Pro Ala Leu Ala Leu Leu Leu Val
165 170 175
Phe Arg Thr Glu Ala Ser Tyr Ser Arg Phe Glu Thr Gly Lys Lys Ala
180 185 190
Trp Thr Lys Val Ile Ala Gly Thr Asn Asp Phe Ala Arg Gln Val Ile
195 200 205
Ala Cys Val Asp Lys Ser Asp Ala Val Leu Lys Glu Ala Leu Leu Gln
210 215 220
Tyr Ile Met Ala Phe Pro Val Ala Leu Lys Cys His Ile Thr Tyr Gly
225 230 235 240
Ser Asp Ile Ala Ser Asp Leu Lys Asn Leu Leu Glu Ala Asp Asp Leu
245 250 255
Ala Leu Val Leu Ser Ser Lys His Arg Pro Arg Cys Val Ile Gly Phe
260 265 270
Ile Ser Gln Cys Leu Gln Ser Leu Asn Leu Glu Gly Thr Lys Leu Thr
275 280 285
Gln Leu Glu Ser Lys Ile Ser Cys Phe His Glu Gly Ile Gly Val Cys
290 295 300
Glu Gln Leu Ala Gly Ile Pro Ile Pro Leu Ser Tyr Thr Arg Leu Thr
305 310 315 320
Ser Arg Phe Leu Val Leu Trp His Leu Thr Leu Pro Ile Ile Leu Trp
325 330 335
Asp Asp Cys His Trp Ile Val Val Pro Ala Thr Phe Ile Ser Ala Ala
340 345 350
Ser Leu Phe Cys Ile Glu Glu Val Gly Val Leu Ile Glu Glu Pro Phe
355 360 365
Pro Met Leu Ala Leu Asp Glu Leu Cys Gln Leu Val Arg Asp Asn Ile
370 375 380
Gln Glu Ala Met Ala Asn Glu Lys Gln Ile Gln Glu Arg Leu Asn Ala
385 390 395 400
Lys Arg Lys Arg Arg Phe Ser Glu His Ser Gln Asn Gly Trp Pro Thr
405 410 415
Ser
<210> 84
<211> 417
<212> PRT
<213> 美花烟草
<400> 84
Met Thr Asn Ser Arg Thr Leu Phe Ser Ile Gln Ser Pro Thr Asn Ala
1 5 10 15
Ser Phe Ser Ser His Phe Thr Leu Lys Leu Pro Ser Lys Leu Gln Gln
20 25 30
Gln Ser Phe Pro Ser Lys Leu Ser Tyr Lys Lys Phe Ser Phe Ser Thr
35 40 45
Phe Lys Val Arg Cys Cys Pro Gln Gln Thr Pro Glu Asn Gln Asn Ser
50 55 60
Thr Ser Ala Leu Ile Ser Ile Leu Arg Ile Ile Pro Asp Trp Ala Asp
65 70 75 80
Arg Ile Gln Glu Glu Gly Met Lys Lys Lys Arg Ser Leu Tyr Lys His
85 90 95
Glu Ser Trp Val Gln His Arg Ser Ser Leu Arg His Val Arg His Leu
100 105 110
Phe Ser Ser Leu Asn Ser Arg Val Ile Leu Ser Leu Val Pro Pro Val
115 120 125
Ile Ala Phe Thr Ser Val Ala Val Val Ile Ala Ser Tyr Asn Ser Ala
130 135 140
Val Ser Met His Trp Leu Pro Glu Phe Phe Pro Val Leu Arg Ala Ser
145 150 155 160
Pro Leu Pro Tyr Gln Leu Thr Ala Pro Ala Leu Ala Leu Leu Leu Val
165 170 175
Phe Arg Thr Glu Ala Ser Tyr Ser Arg Phe Glu Thr Gly Lys Lys Ala
180 185 190
Trp Thr Lys Val Ile Ala Gly Thr Asn Asp Phe Ala Arg Gln Val Ile
195 200 205
Ala Cys Val Asp Lys Ser Asp Ala Val Leu Lys Glu Ala Leu Leu Gln
210 215 220
Tyr Ile Met Ala Phe Pro Val Ala Leu Lys Cys His Ile Thr Tyr Gly
225 230 235 240
Ser Asp Ile Ala Ser Asp Leu Lys Asn Leu Leu Glu Ala Asp Asp Leu
245 250 255
Ala Leu Val Leu Ser Ser Lys His Arg Pro Arg Cys Val Ile Gly Phe
260 265 270
Ile Ser Gln Cys Leu Gln Ser Leu Asn Leu Glu Gly Thr Lys Leu Thr
275 280 285
Gln Leu Glu Ser Lys Ile Ser Cys Phe His Glu Gly Ile Gly Val Cys
290 295 300
Glu Gln Leu Ala Gly Ile Pro Ile Pro Leu Ser Tyr Thr Arg Leu Thr
305 310 315 320
Ser Arg Phe Leu Val Leu Trp His Leu Thr Leu Pro Ile Ile Leu Trp
325 330 335
Asp Asp Cys His Trp Ile Val Val Pro Ala Thr Phe Ile Ser Ala Ala
340 345 350
Ser Leu Phe Cys Ile Glu Glu Val Gly Val Leu Ile Glu Glu Pro Phe
355 360 365
Pro Met Leu Ala Leu Asp Glu Leu Cys Gln Leu Val Arg Asp Asn Ile
370 375 380
Gln Glu Ala Met Ala Asn Glu Lys Gln Ile Gln Glu Arg Leu Asn Ala
385 390 395 400
Lys Arg Lys Lys Cys Phe Ser Glu His Ser Pro Asn Gly Trp Pro Thr
405 410 415
Ser
<210> 85
<211> 417
<212> PRT
<213> 烟草
<400> 85
Met Thr Asn Ser Arg Thr Leu Phe Ser Ile Gln Ser Pro Thr Asn Ala
1 5 10 15
Ser Phe Ser Ser His Phe Thr Leu Lys Leu Pro Ser Lys Leu Gln Gln
20 25 30
Gln Ser Phe Pro Ser Lys Leu Ser Tyr Lys Lys Phe Ser Phe Ser Thr
35 40 45
Phe Lys Val Arg Cys Cys Pro Gln Gln Thr Pro Glu Asn Gln Asn Ser
50 55 60
Thr Ser Ala Leu Ile Ser Ile Leu Arg Ile Ile Pro Asp Trp Ala Asp
65 70 75 80
Arg Ile Gln Glu Glu Gly Met Lys Lys Lys Arg Ser Leu Tyr Lys His
85 90 95
Glu Ser Trp Val Gln His Arg Ser Ser Leu Arg His Val Arg His Leu
100 105 110
Phe Ser Ser Leu Asn Ser Arg Val Ile Leu Ser Leu Val Thr Pro Val
115 120 125
Ile Ala Phe Thr Ser Val Ala Val Val Ile Ala Ser Tyr Asn Ser Ala
130 135 140
Val Ser Met His Trp Leu Pro Glu Phe Phe Pro Val Leu Arg Ala Ser
145 150 155 160
Pro Leu Pro Tyr Gln Leu Thr Ala Pro Ala Leu Ala Leu Leu Leu Val
165 170 175
Phe Arg Thr Glu Ala Ser Tyr Ser Arg Phe Glu Thr Gly Lys Lys Ala
180 185 190
Trp Thr Lys Val Ile Ala Gly Thr Asn Asp Phe Ala Arg Gln Val Ile
195 200 205
Ala Cys Val Asp Lys Ser Asp Ala Val Leu Lys Glu Ala Leu Leu Gln
210 215 220
Tyr Ile Met Ala Phe Pro Val Ala Leu Lys Cys His Ile Thr Tyr Gly
225 230 235 240
Ser Asp Ile Ala Ser Asp Leu Lys Asn Leu Leu Glu Ala Asp Asp Leu
245 250 255
Ala Leu Val Leu Ser Ser Lys His Arg Pro Arg Cys Val Ile Gly Phe
260 265 270
Ile Ser Gln Cys Leu Gln Ser Leu Asn Leu Glu Gly Thr Lys Leu Thr
275 280 285
Gln Leu Glu Ser Lys Ile Ser Cys Phe His Glu Gly Ile Gly Val Cys
290 295 300
Glu Gln Leu Ala Gly Ile Pro Ile Pro Leu Ser Tyr Thr Arg Leu Thr
305 310 315 320
Ser Arg Phe Leu Val Leu Trp His Leu Thr Leu Pro Ile Ile Leu Trp
325 330 335
Asp Asp Cys His Trp Ile Val Val Pro Ala Thr Phe Ile Ser Ala Ala
340 345 350
Ser Leu Phe Cys Ile Glu Glu Val Gly Val Leu Ile Glu Glu Pro Phe
355 360 365
Pro Met Leu Ala Leu Asp Glu Leu Cys Gln Leu Val Arg Asp Asn Ile
370 375 380
Gln Glu Ala Met Ala Asn Glu Lys Gln Ile Gln Glu Arg Leu Asn Ala
385 390 395 400
Lys Arg Lys Lys Cys Phe Ser Glu His Ser Pro Asn Gly Trp Pro Thr
405 410 415
Ser
<210> 86
<211> 417
<212> PRT
<213> 绒毛状烟草
<400> 86
Met Thr Asn Ser Arg Thr Leu Phe Ser Ile Gln Ser Pro Thr Asn Ala
1 5 10 15
Ser Phe Ser Ser His Phe Thr Leu Lys Thr Pro Ser Lys Leu Gln Gln
20 25 30
Gln Ser Phe Pro Ser Lys Leu Asn Phe Lys Lys Leu Arg Phe Ser Thr
35 40 45
Phe Lys Val Arg Cys Cys Pro Gln Gln Thr Pro Gln Asn Gln Asn Pro
50 55 60
Thr Ser Ala Leu Ile Ser Ile Leu Arg Ile Ile Pro Asp Trp Ala Asp
65 70 75 80
Arg Ile Gln Glu Glu Gly Met Lys Lys Lys Arg Ser Leu Tyr Thr His
85 90 95
Glu Ser Trp Met Gln His Arg Ser Ser Leu Arg His Val Arg His Leu
100 105 110
Phe Ser Ser Leu Asn Ser Arg Val Ile Leu Ser Leu Val Pro Pro Val
115 120 125
Ile Ala Phe Thr Ser Val Ala Val Val Ile Ala Ser Tyr Asn Ser Ala
130 135 140
Val Ser Met His Trp Leu Pro Glu Leu Phe Pro Val Leu Arg Ala Ser
145 150 155 160
Pro Leu Pro Tyr Gln Leu Thr Ala Pro Ala Leu Ala Leu Leu Leu Val
165 170 175
Phe Arg Thr Glu Ala Ser Tyr Ser Arg Phe Glu Thr Gly Lys Lys Ala
180 185 190
Trp Thr Lys Val Ile Ala Gly Thr Asn Asp Phe Ala Arg Gln Val Ile
195 200 205
Ala Cys Val Asp Lys Ser Asp Ala Val Leu Lys Ala Ala Leu Leu Gln
210 215 220
Tyr Ile Met Ala Phe Pro Val Ala Leu Lys Cys His Ile Thr Tyr Gly
225 230 235 240
Ser Asp Ile Ala Ser Asp Leu Lys Asn Leu Leu Glu Ala Asp Asp Leu
245 250 255
Ala Val Val Leu Ser Ser Lys His Arg Pro Arg Cys Ile Ile Gly Phe
260 265 270
Ile Ser Gln Cys Leu Gln Ser Leu His Leu Glu Gly Thr Lys Leu Thr
275 280 285
Gln Leu Glu Ser Lys Ile Ser Cys Phe His Glu Gly Ile Gly Val Cys
290 295 300
Glu Gln Leu Ala Gly Ile Pro Ile Pro Leu Ser Tyr Thr Arg Leu Thr
305 310 315 320
Ser Arg Phe Leu Val Leu Trp His Leu Thr Leu Pro Ile Ile Leu Trp
325 330 335
Asp Asp Cys His Trp Ile Val Val Pro Ala Thr Phe Ile Ser Ala Ala
340 345 350
Ser Leu Phe Cys Ile Glu Glu Val Gly Val Leu Ile Glu Glu Pro Phe
355 360 365
Pro Met Leu Ala Leu Asp Glu Leu Cys Gln Leu Val His Asp Asn Ile
370 375 380
Gln Glu Ser Met Ala Asn Glu Lys Lys Ile Gln Glu Arg Leu Ser Ala
385 390 395 400
Lys Arg Lys Arg Arg Phe Ser Glu His Ser Gln Asn Gly Trp Pro Thr
405 410 415
Ser
<210> 87
<211> 412
<212> PRT
<213> 潘那利番茄
<400> 87
Met Thr Asn Pro Arg Thr Leu Phe Thr Ile Gln Ser Pro Thr Asn Ala
1 5 10 15
Ser Phe Ser Ser His Phe Thr Leu Lys Asn Pro Ser Lys Ser Gln Gln
20 25 30
Gln Gln Pro Val Tyr Lys Lys Leu Asn Phe Arg Val Leu Cys Ser Ser
35 40 45
Gln Pro Ser Arg Thr Pro Gln Asn Ser Asn Leu Ile Ser Thr Leu Val
50 55 60
Lys Ile Leu Arg Val Val Pro Asp Trp Ala Asp Lys Ile Gln Glu Gly
65 70 75 80
Gly Met Arg Lys Lys Arg Ser Leu Tyr Lys His Glu Thr Trp Val Gln
85 90 95
His Arg Ser Ser Leu Arg His Val Arg His Leu Phe Ser Ser Phe Asn
100 105 110
Ser Arg Val Val Leu Ser Leu Ile Pro Pro Val Ile Ala Phe Thr Ser
115 120 125
Phe Ala Phe Val Ile Ala Ser Tyr Asn Ser Ala Val Ser Phe His Trp
130 135 140
Leu Pro Glu Phe Phe Pro Ile Leu Arg Ala Ser Pro Gln Pro Tyr Gln
145 150 155 160
Leu Thr Ala Pro Ala Leu Ala Leu Leu Leu Val Phe Arg Thr Glu Ala
165 170 175
Ser Tyr Ser Arg Phe Glu Ala Gly Lys Lys Ala Trp Thr Lys Val Ile
180 185 190
Ala Gly Thr Asn Asp Phe Ala Arg Gln Val Ile Ala Cys Val Asp Lys
195 200 205
Arg Asp Asp Val Leu Lys Glu Ala Leu Leu Gln Tyr Ile Met Ala Phe
210 215 220
Pro Val Ala Leu Lys Cys His Ile Val Tyr Asp Ser Asp Ile Ala Ser
225 230 235 240
Asp Leu Lys Asn Leu Leu Glu Ala Asp Asp Leu Ala Val Val Leu Ser
245 250 255
Ser Lys His Arg Pro Arg Cys Ile Ile Gly Phe Ile Ala Gln Ser Leu
260 265 270
Gln Ser Leu Asn Leu Glu Gly Thr Ile Leu Ser Gln Leu Glu Ser Lys
275 280 285
Ile Ser Cys Phe His Glu Gly Ile Gly Val Cys Glu Gln Leu Leu Gly
290 295 300
Ile Pro Ile Pro Leu Ser Tyr Thr Arg Leu Thr Ser Arg Phe Leu Val
305 310 315 320
Leu Trp His Leu Thr Leu Pro Ile Ile Leu Trp Asp Asp Cys His Trp
325 330 335
Ile Val Val Pro Ala Thr Phe Ile Ser Ala Ala Ser Leu Phe Cys Ile
340 345 350
Glu Glu Val Gly Val Leu Ile Glu Glu Pro Phe Pro Met Leu Ala Leu
355 360 365
Asp Glu Leu Cys Gln Arg Val His Asp Asn Ile His Glu Ala Met Ala
370 375 380
Asn Glu Lys Gln Ile Gln Glu Ile Val Asn Ser Lys Arg Lys Arg Ser
385 390 395 400
Phe Ser Glu His Ser Pro Asn Gly Trp Pro Thr Ser
405 410
<210> 88
<211> 412
<212> PRT
<213> 马铃薯
<400> 88
Met Thr Asn Pro Arg Thr Phe Phe Thr Ile Gln Ser Pro Thr Asn Ala
1 5 10 15
Ser Phe Ser Ser His Phe Thr Leu Lys Lys Pro Ser Asn Ser Gln Gln
20 25 30
Gln Pro Pro Val Tyr Lys Lys Phe Thr Phe Arg Val Leu Cys Ser Ser
35 40 45
Gln Pro Ser Arg Thr Pro Gln Asn Ser Asn Leu Ile Ser Thr Leu Val
50 55 60
Lys Ile Leu Arg Val Val Pro Asp Trp Ala Asp Lys Ile Gln Glu Gly
65 70 75 80
Gly Met Arg Lys Lys Arg Ser Leu Tyr Lys His Glu Thr Trp Val Gln
85 90 95
His Arg Ser Ser Leu Arg His Leu Arg His Leu Phe Ser Ser Phe Asn
100 105 110
Ser Arg Val Val Leu Ser Leu Ile Pro Pro Val Ile Ala Phe Thr Ser
115 120 125
Phe Ala Phe Val Ile Ala Cys Tyr Asn Ser Ala Val Ser Leu His Trp
130 135 140
Leu Pro Glu Phe Phe Pro Ile Leu Arg Ala Ser Pro Gln Pro Tyr Gln
145 150 155 160
Leu Thr Ala Pro Ala Leu Ala Leu Leu Leu Val Phe Arg Thr Glu Ala
165 170 175
Ser Tyr Ser Arg Phe Glu Ala Gly Lys Lys Ala Trp Thr Lys Val Ile
180 185 190
Ala Gly Thr Asn Asp Phe Ala Arg Gln Val Ile Ala Cys Val Asp Lys
195 200 205
Arg Asp Asp Val Leu Lys Glu Ala Leu Leu Gln Tyr Ile Met Ala Phe
210 215 220
Pro Val Ala Leu Lys Cys His Ile Val Tyr Asp Ser Asp Ile Ala Ser
225 230 235 240
Asp Leu Lys Asn Leu Leu Glu Ala Asp Asp Leu Ala Leu Val Val Ser
245 250 255
Ser Lys His Arg Pro Arg Cys Ile Ile Gly Phe Ile Ser Gln Ser Leu
260 265 270
Gln Ser Leu Asn Leu Asp Gly Thr Ile Leu Ser Gln Leu Glu Ser Lys
275 280 285
Ile Thr Cys Phe His Glu Gly Ile Gly Val Cys Glu Gln Leu Leu Gly
290 295 300
Ile Pro Ile Pro Leu Ser Tyr Thr Arg Leu Thr Ser Arg Phe Leu Val
305 310 315 320
Leu Trp His Leu Thr Leu Pro Ile Ile Leu Trp Asp Asp Cys His Trp
325 330 335
Ile Val Val Pro Ala Thr Phe Ile Ser Ala Ala Ser Leu Phe Cys Ile
340 345 350
Glu Glu Val Gly Val Leu Ile Glu Glu Pro Phe Pro Met Leu Ala Leu
355 360 365
Asp Glu Leu Cys Gln Arg Val His Asp Asn Ile His Glu Ala Met Ala
370 375 380
Asn Glu Lys Gln Ile Gln Glu Ile Val Asn Ser Lys Arg Lys Arg Ser
385 390 395 400
Phe Ser Glu His Ser Pro Asn Gly Trp Pro Thr Ser
405 410
<210> 89
<211> 481
<212> PRT
<213> 小球藻
<400> 89
Met Val Val Ala Ala Cys Arg Gly His Ala Met Leu Ser Asp Ala Ala
1 5 10 15
Gln Arg Arg Gly Pro Ala Ala Gly Gln Gly Pro Ala Ala Ala Ala Ala
20 25 30
Ile Arg Gly Leu Ser Pro Cys Arg Arg Ser Ala Ser Thr Trp Arg Leu
35 40 45
Val Pro Ala Ala Gln Arg Arg Pro Arg Pro Pro Met Met Asp Ser Ile
50 55 60
Leu Asp Asp Leu Gly Ser Asn Gly Asp Ser Ala Ala Glu Ser Ser Thr
65 70 75 80
Asp Leu Ala Ala Ala Glu Pro Pro Ala Val Phe Pro Pro Leu Leu Ser
85 90 95
Val Asp Leu Ala Ser Asp Pro Val Lys Glu Gly Ser Arg Arg Tyr Arg
100 105 110
Arg Thr Val Phe Asp Phe Asn Glu Trp Arg Lys His Arg Ser Thr Glu
115 120 125
Arg Tyr Val Arg His Met Gln Ser Met Thr Ser Ser Arg Met Val Arg
130 135 140
Gly Leu Ala Ala Pro Leu Ala Tyr Ile Phe Ala Val Ala Thr Ala Val
145 150 155 160
Ser Val Tyr Asn Ala Ser Val Glu Ala Gly Leu Leu Pro Ser Leu Leu
165 170 175
Pro Glu Val Lys Met Ala Ser Asn Gly Pro Phe Gly Ser Val Ser Phe
180 185 190
Ala Ile Ser Leu Leu Leu Val Phe Arg Thr Asn Ala Ser Tyr Ala Arg
195 200 205
Trp Leu Asp Ala Arg Lys Ala Trp Gly Lys Leu Ile Asn Arg Ser Arg
210 215 220
Asp Leu Thr Arg Gln Ala Leu Thr Tyr Phe Pro Pro Glu Asp Lys Pro
225 230 235 240
Leu Leu Asp Leu Leu Cys Arg Trp Thr Val Ala Phe Ser Arg Thr Leu
245 250 255
Lys Cys His Leu Cys Glu Asp Tyr Pro Leu Ala Glu Glu Leu Ala Arg
260 265 270
Val Leu Arg Pro His Glu Ala Glu Ala Leu Leu Leu Ala Lys His Arg
275 280 285
Pro Thr Tyr Cys Leu Gln Ile Leu Ser Glu Thr Leu Lys Ser Ala His
290 295 300
Leu Pro Pro Pro Leu Pro Val Ala Gln Pro His Asn Gly Ala Thr Ala
305 310 315 320
Gly His Ala Ala Ala Ala Gln Ala Val Ala Ala Val Pro Ala Ala Ala
325 330 335
Ala Tyr Arg Met Asp Glu Asn Leu Thr Glu Phe Glu Asp Ile Val Gly
340 345 350
Thr Cys Glu Arg Ile Leu Arg Ala Pro Ile Pro Leu Ser Tyr Thr Arg
355 360 365
His Thr Ser Arg Phe Met Ile Ile Trp Leu Thr Leu Leu Pro Phe Ala
370 375 380
Leu Trp Asp Ser Val Gly Trp Ala Thr Val Pro Leu Ala Val Ile Val
385 390 395 400
Ala Phe Leu Leu Leu Gly Ile Glu Glu Ile Gly Val Ser Ile Glu Glu
405 410 415
Pro Phe Ser Ile Leu Pro Leu Asp Ala Leu Cys Asp Thr Ile Glu Ala
420 425 430
Asn Val Lys Glu Leu Gln Arg Thr His Ser Gln Gln Gly Gly Gly Gly
435 440 445
Gly Glu Glu Gly Ser His Lys Ala Arg Pro Pro Pro Ala Arg Asp Ile
450 455 460
Val Gly Met Ala Ala Ala Glu Ala Glu Ala Glu Glu Ala Ala Val Ala
465 470 475 480
Lys
<210> 90
<211> 590
<212> PRT
<213> 小球藻
<400> 90
Met Ser Ala Leu Leu Arg Ala Arg Val Ser Ala Phe Leu Ala Gly Val
1 5 10 15
Ala Val Ala Gly Val Phe Gly Val Tyr Gln Leu Arg Gly Asp Val Ala
20 25 30
Glu Gly Gln Gln Arg Leu Leu Asp Gln Thr Lys Lys Tyr Thr Ala Gly
35 40 45
Leu Glu Gly Arg Val Ala Glu Leu Glu Ala Ala Val Ala Arg Leu Ser
50 55 60
Ser Asn Arg Ala Leu Lys Pro Asn Ser Ala Ala Pro Pro Pro Ala Pro
65 70 75 80
Ala Met Pro Ala Gly Leu Met Thr Gln Gln Ser Thr Ala Cys Ser Ala
85 90 95
Ala Ala Ala Ser Gly Arg Leu Gln Gly Ala Ala Arg Ser Leu Pro Arg
100 105 110
Arg His Ala Leu Ser Gly Ala Ala Val Gly Arg Pro Ala Pro Ala Gly
115 120 125
Leu Gly Arg Gly Leu Ser Ser Ala Ser Arg Ala Gln Gly Arg Arg Ala
130 135 140
Gly Ala Val Arg Val Gln Ala Ala Ala Thr Pro Gly Val Pro Gly Ala
145 150 155 160
Asn Ser Ala Ser Glu Gly Pro Phe Asp Trp Met Thr Asp Glu Trp Lys
165 170 175
Glu Ala Asp Arg Lys Asn Leu Arg Thr Val Phe Asp Phe Asp Ala Trp
180 185 190
Lys Arg His Arg Ser Ser Ser Arg Tyr Trp Arg His Leu Gly His Leu
195 200 205
Pro Asn Ser Arg Ile Leu Lys Trp Val Ala Gly Pro Leu Ser Tyr Val
210 215 220
Thr Ala Val Ala Ile Gly Val Cys Ala Tyr His Thr Ala Ala Glu Ala
225 230 235 240
Gly Ile Val Pro Glu Val Leu Pro Glu Leu Ala Lys Gly Ala Ala Ala
245 250 255
Pro Phe Gly Leu Thr Ser Phe Ala Leu Ser Thr Leu Leu Val Leu Arg
260 265 270
Thr Asn Thr Ser Tyr Gln Arg Trp Asp Glu Ala Arg Lys Met Trp Gly
275 280 285
Leu Ile Val Asn Arg Ser Arg Asp Val Ala Arg Gln Ala Leu Gly Tyr
290 295 300
Ile Pro Ala Ser Gln Pro Glu Leu Gln Asp Met Phe Cys Arg Trp Leu
305 310 315 320
Val Ala Tyr Cys Arg Ser Leu Met Cys His Leu Arg Pro Gly Glu Asp
325 330 335
Leu Arg Ala Glu Leu Glu Gly Lys Leu Lys Pro Glu Glu Leu Glu Ala
340 345 350
Leu Leu Ser Ser Ala His Arg Pro Asn Tyr Thr Cys Gln Val Leu Thr
355 360 365
Ala Val Ile Lys Arg Ala Gln Leu Pro Gly Asp Asp Ile Asp Asn Arg
370 375 380
Asp Ser Phe Ala Asn Val Lys Ala Ser Ala Ala Phe Arg Met Asp Glu
385 390 395 400
Asn Leu Thr Gln Phe Ala Asp Val Thr Gly Gly Cys Glu Arg Ile Leu
405 410 415
Arg Thr Pro Val Pro Leu Met Tyr Thr Arg His Asn Ser Arg Phe Leu
420 425 430
Met Ile Trp Leu Ser Leu Leu Pro Phe Thr Leu Trp Asp Thr Cys His
435 440 445
Trp Ala Thr Val Pro Val Thr Ala Leu Val Ala Phe Leu Leu Leu Gly
450 455 460
Ile Lys Glu Val Gly Val Ala Ile Glu Glu Pro Phe Thr Ile Leu Pro
465 470 475 480
Leu Glu Val Ile Cys Asn Thr Ile Glu Gly Asn Val Trp Glu Leu His
485 490 495
Arg Met His Ser Ala Asn Gly Val Glu Gln Gln His Ala Ala Gly Asn
500 505 510
Ala Val Val Thr Ala Glu Ala Leu Val Asp Ile Met Ala Pro Gln Thr
515 520 525
Ile Gly Gly Leu Ala Leu Asn Gly Ala Gly Ser Ala Ala Tyr Val Asn
530 535 540
Gly Asn Gly Asn Gly Ala Tyr Thr Asn Gly Ala Ala Asn Gly Asn Gly
545 550 555 560
Ala Glu Ser Gly Thr Ile Lys Val Pro Pro Glu Glu Leu Ile Arg Ala
565 570 575
Ala Gln Gly Leu Arg Gln Arg Val Ser Ser Ser Leu Arg Gln
580 585 590
<210> 91
<211> 450
<212> PRT
<213> 小球藻
<400> 91
Met Ala Thr Pro Ala Ala Ala Ala Pro Arg Arg Leu Gly Ala Ala Ala
1 5 10 15
Ala Pro Lys Gly Leu Pro Arg Leu Ala Gly Val Ser Ala Arg Pro Arg
20 25 30
Arg Ala Ala Gln Thr Ala Gly Ser Phe His Ser Ala Ala Leu Pro Val
35 40 45
Asp Gln Ala Val Asn Gly Asn Gly Thr Val Pro Pro Asp Pro Glu Pro
50 55 60
Glu Pro Thr Tyr Ser Trp Phe Ser Gln Glu Trp Lys Glu Glu Arg Arg
65 70 75 80
Lys Lys Lys Gly Arg Thr Val Phe Asp Phe Glu Arg Trp Gln Lys His
85 90 95
Arg Ser Ser Ala Arg Tyr Leu Arg His Met Ala Gly Met Phe Ser Ser
100 105 110
Ser Val Ile Gln Gly Leu Ala Val Pro Leu Ala Tyr Val Val Thr Val
115 120 125
Ser Val Gly Val Ala Thr Tyr Tyr Thr Ala Ala Ala Lys Gly Met Val
130 135 140
Pro Leu Phe Pro Ala Leu Lys Val Leu Ala Ser Ala Pro Phe Ser Leu
145 150 155 160
Thr Ser Phe Ala Leu Ser Leu Leu Leu Val Phe Arg Thr Asn Ser Ser
165 170 175
Tyr Gly Arg Trp Asp Glu Ala Arg Lys Met Trp Gly Gln Val Val Asn
180 185 190
Arg Ser Arg Asp Ile Ala Arg Gln Ala Leu Ala Tyr Ile Pro Ala Ala
195 200 205
Gln Val His Leu Gln Asp Val Ile Cys Arg Trp Thr Ile Ala Tyr Thr
210 215 220
Arg Ala Leu Met Cys His Leu Arg Gln Gly Glu Asp Leu Gly Ser Glu
225 230 235 240
Leu Ala Gly Ile Leu Ser Pro Ser Glu Leu Ala Ala Leu Leu Ala Ala
245 250 255
Lys His Arg Pro Asn Phe Cys Thr Gln Val Leu Ser Ala Ala Val Arg
260 265 270
Asp Ala Gln Leu Pro Gly Thr Gly Pro Thr Ser Arg Glu Pro Thr Ala
275 280 285
Ala Val Pro Ala Gly Ala Ala Tyr Arg Met Asp Glu Asn Ile Thr Val
290 295 300
Phe Val Glu Val Ser Gly Gly Cys Glu Arg Ile Leu Arg Thr Pro Ile
305 310 315 320
Pro Leu Gly Tyr Thr Arg His Thr Ser Arg Phe Leu Met Leu Trp Leu
325 330 335
Thr Leu Leu Pro Phe Ser Leu Trp Glu Ser Cys Gly Leu Ala Met Ile
340 345 350
Pro Val Ala Ala Leu Val Ala Phe Leu Leu Leu Gly Val Glu Glu Ile
355 360 365
Gly Val Gln Ile Glu Glu Pro Phe Ser Ile Leu Pro Leu Glu Val Ile
370 375 380
Ser Ser Thr Ile Glu Gly Asn Ile Arg Glu Leu Gln Ala Thr His Gly
385 390 395 400
Ala Ala Ala Ala Val Pro Ser Pro Ile His Asp Ile Ile Arg Pro Pro
405 410 415
Gln Leu Gln Ser Pro Glu Gly Asn Ser Ser Asn Val Val Gly Ala Pro
420 425 430
Ser Leu Ala Thr Asn Gly Ala Pro Ala Gly Arg Pro Ser Gly Gly Gly
435 440 445
Ala Arg
450
<210> 92
<211> 408
<212> PRT
<213> 小球藻
<400> 92
Met Gln Lys Arg Thr Arg Pro Arg Ser Arg Leu Pro Pro Val Thr Leu
1 5 10 15
Cys Cys Arg Leu Pro Pro Ala Ala Gln Pro Pro Arg Gly Val His Ala
20 25 30
Ala Gln Ala Leu Leu Arg Pro Ala Gly Cys Ser Gly Ser Gln Gln Gln
35 40 45
Arg Arg Arg Arg Arg Pro Pro Ser Thr Ala Ala Ser Leu Leu Pro Leu
50 55 60
Gly Asp Arg Asp Glu Asp Lys Glu Glu Arg Leu Arg Arg Phe Arg Val
65 70 75 80
Met Tyr Asp Phe Asp Ala Trp Ala Ala His Arg Ser Val Lys Arg Tyr
85 90 95
Trp Arg His Phe Ile Gly Leu Phe Arg Ser Arg Ile Val Ala Gly Ile
100 105 110
Ala Leu Pro Ile Leu Leu Val Phe Leu Glu Ala Thr Gly Val Cys Trp
115 120 125
Tyr Glu Thr Ala Leu Arg Ser Gly Leu Leu Pro Ala Thr Trp Arg Ser
130 135 140
Ile Gln Val Arg Ala Pro Met Leu Phe Ser Leu Ser Ser Phe Ala Leu
145 150 155 160
Ser Leu Leu Leu Gly Phe Arg Thr Asn Gln Ser Ile Ala Arg Phe Asp
165 170 175
Glu Ala Arg Arg Leu Trp Asn Thr Met Ile Asn Arg Cys Arg Asp Ala
180 185 190
Met Arg Gln Ala Val Ser Tyr Leu Gly Pro Gln Leu Pro Gln Gln Ala
195 200 205
Ala Leu Leu Gly Ala Leu Ser Arg Trp Leu Gln Ala Phe Pro Trp Val
210 215 220
Leu Lys Asn His Val Arg Glu His Lys His Trp Gln Ala Glu Leu Gly
225 230 235 240
Asp Ile Leu Glu Pro Ser Glu Leu Glu Ala Val Val Ala Ser Lys His
245 250 255
Pro Pro Leu Leu Ala Leu Gln Val Ile Thr Glu Leu Leu Asp Arg Ala
260 265 270
Pro Ile Ser Ala Lys Gln Arg Leu Arg Ile Asp Gly Cys Val Thr Asp
275 280 285
Leu Ser Asp Val Val Gly Gly Cys Gln Arg Ile Leu Asn Thr Pro Ile
290 295 300
Pro Val Ser Tyr Thr Arg His Thr Cys Arg Tyr Leu Thr Val Trp Leu
305 310 315 320
Phe Leu Leu Pro Leu Ser Ile Phe Asn Gln Cys Gly Trp Val Thr Val
325 330 335
Phe Ser Ser Val Val Leu Ala Phe Phe Leu Leu Thr Ile Glu Glu Ile
340 345 350
Gly Val Thr Ile Glu Glu Pro Phe Asn Ile Leu Pro Leu Ala Glu Leu
355 360 365
Asn Arg Gly Ala Asp Val Ala Gln Asn Leu Asp Arg Ile Asp Glu Val
370 375 380
Val Ala Leu Val Asp Ala Ala Thr Gly Ser Arg Arg Thr Ala Met Gln
385 390 395 400
Leu Ala Pro Ala Tyr Pro Ile Glu
405
<210> 93
<211> 456
<212> PRT
<213> 小球藻
<400> 93
Met Ser Thr Ala Met Leu Ala Gly Ser Arg Ile Gln Leu Gln Gln Pro
1 5 10 15
Ala Gly Leu Gly Gly Ser Arg Leu Gln Arg Ala Ala Ala Pro Val Ala
20 25 30
Ala Ala Ala Arg Leu Gly Ser Val Arg Pro Ala Gly Leu Gln Ala Arg
35 40 45
Ser Thr Ala Ala Arg Arg Ala Asp Arg Ser Ala Leu Arg Val Ser Ala
50 55 60
Thr Ala Ser Pro Glu Ala Ala Pro Val Lys Leu Ser Gly Asp Asp Leu
65 70 75 80
Lys Glu Ala Asn Arg Lys His Met Arg Ser Val Phe Asp Phe Asp Leu
85 90 95
Trp Lys Lys His Arg Ser Ser Ser Arg Tyr Leu Arg His Ile Val Gly
100 105 110
Leu Gly Glu Ser Arg Ile Val Ser Gly Leu Met Ala Pro Leu Thr Tyr
115 120 125
Val Met Thr Leu Ser Leu Ala Val Ala Cys Tyr Asn Ala Ala Ala Glu
130 135 140
Ala Gly Tyr Leu Pro Val Phe Pro Glu Leu Lys Leu Ala Thr Asn Ala
145 150 155 160
Pro Phe Gly Leu Thr Ser Phe Ala Leu Ser Leu Leu Leu Val Phe Arg
165 170 175
Thr Asn Ser Ser Tyr Gly Arg Trp Asp Glu Ala Arg Lys Met Trp Gly
180 185 190
Leu Ile Val Asn Arg Ser Arg Asp Phe Ile Arg Gln Gly Leu Gly Tyr
195 200 205
Ile Pro Pro Glu Gln Glu Glu Leu Gln Lys Met Leu Val Arg Trp Thr
210 215 220
Val Ala Tyr Ser Arg Ser Leu Met Cys His Leu Arg Pro Gly Glu Asp
225 230 235 240
Leu Arg Val Glu Leu Lys Asp Thr Leu Lys Pro Glu Glu Leu Glu Ala
245 250 255
Leu Leu Ala Ser Thr His Arg Pro Asn Tyr Val Val Gln Val Leu Thr
260 265 270
Ala Ile Ile Lys Thr Ala Gln Leu Pro Ala Ala Val Thr Asn Asn Arg
275 280 285
Asp Ser Thr Gly Cys Val Pro Ala Gly Ala Ala Tyr Arg Met Asp Glu
290 295 300
Asn Leu Thr Val Phe Ala Asp Val Thr Gly Gly Cys Glu Arg Ile Leu
305 310 315 320
Arg Thr Pro Ile Pro Leu Ser Tyr Thr Arg His Thr Ser Arg Phe Met
325 330 335
Met Ile Trp Leu Thr Leu Leu Pro Phe Thr Leu Trp Asp Ser Cys His
340 345 350
Trp Ala Met Leu Pro Ile Ala Gly Ile Val Ser Phe Leu Leu Leu Gly
355 360 365
Ile Glu Glu Ile Gly Val Gln Ile Glu Glu Pro Phe Thr Ile Leu Pro
370 375 380
Leu Glu Val Ile Ser Arg Thr Ile Glu Gly Asn Val Trp Glu Leu Tyr
385 390 395 400
Arg Met His Ser Gly Glu Ala Leu Glu Lys Glu Gln Ala Glu Leu Ala
405 410 415
Asn Gly Gln Asp Val Gln Val Leu Asn Ala Gln Asp Leu Val Ala Leu
420 425 430
Met Ala Pro Ser Ala Val Gly Asn Thr Ala Asn Gly Thr Ser Arg Lys
435 440 445
Ser Leu Val Val Asn Tyr Gly Leu
450 455
<210> 94
<211> 589
<212> PRT
<213> 小球藻
<400> 94
Met Gly Leu Met Pro Asn Met Glu Asn Thr Tyr Ser His Val Pro Arg
1 5 10 15
Ser Ala Met Ala Ala Met Ala Ala Ser Ala Pro Ala Asp Ala Ser Thr
20 25 30
Asn Ala Gly Gly Ser Pro Gly Val Gly Arg Leu Ala Ala Arg Thr Ser
35 40 45
Ser Glu Pro Pro Ser Gly Val His Ser Arg Ser Ser Ser Leu Thr Asn
50 55 60
Leu Ala Arg Leu Pro Gly Lys Ala Arg Ala Lys Leu Gln Asn Leu Thr
65 70 75 80
His Ser Gln Phe Val Arg Phe Ile Glu Asp Asn Asp Ala Gln Asp Lys
85 90 95
Glu Glu Asn Asp Ile Tyr Arg Gly Ala Ala Ala Ala Arg Gln Asn Pro
100 105 110
Ala Asp Gln Val Phe Thr Phe Glu Arg Trp Asn Lys His Arg Asn Pro
115 120 125
Met Arg Tyr Ala Lys His Leu Leu His Met Phe Thr Ser Arg Val Phe
130 135 140
Lys Gln Leu Leu Gly Pro Val Leu Ala Val Met Ala Leu Ala Leu Ser
145 150 155 160
Val Gly Ile Tyr Glu Thr Leu Val Ser Ala Gly Ala Leu Pro Gly His
165 170 175
Trp Pro His Val Thr Leu Ala Leu Gly Gln Gly Phe Asn Leu Thr Ala
180 185 190
Phe Ala Leu Ser Leu Leu Leu Val Phe Arg Thr Asn Ser Ser Tyr Asp
195 200 205
Arg Trp Trp Glu Gly Arg Lys Leu Trp Gly Gly Val Val Asn Arg Thr
210 215 220
Arg Asn Ile Val Arg Gln Ser Leu Val Phe Phe Arg Glu Asp Val His
225 230 235 240
Leu Lys Glu Leu Leu Ala Arg Trp Thr Met Ala Phe Pro Lys Val Leu
245 250 255
Met Val His Leu Arg Glu Gly Met Asp Leu Gln Ala Glu Val Ala His
260 265 270
Ile Leu Thr Ala Asn Glu Val Ala Ala Leu Val Ala Ala Ala His Arg
275 280 285
Pro Asn Phe Val Leu Gln Val Met Ala Glu Ala Ile Arg Ala Ala Arg
290 295 300
Pro His Glu Leu Ala Arg Met Arg Met Asp Asp Asn Leu Thr Phe Phe
305 310 315 320
Glu Asp Ala Met Gly Ser Cys Glu Arg Ile Leu Arg Thr Pro Ile Pro
325 330 335
Leu Ser Tyr Thr Arg His Thr Ser Arg Phe Leu Leu Val Trp Leu Ile
340 345 350
Leu Leu Pro Phe Thr Leu Trp Ala Ala Tyr Ser Trp Phe Ala Val Ile
355 360 365
Leu Ser Gly Ile Phe Ala Phe Leu Met Phe Gly Ile Asp Glu Ile Gly
370 375 380
Val Gln Ile Glu Glu Pro Phe Gly Ile Leu Pro Leu Glu Ala Ala Gly
385 390 395 400
Ala Thr Ile Glu Arg Asn Ile Arg Glu Leu Leu Ala Arg Asn Tyr Glu
405 410 415
Val Ala Ser Leu Val His Gln Gln Asn Leu Pro Val Gly Leu Gly Asp
420 425 430
Ser Arg Gly Lys Gln Pro Ala Gly Ser Ser Pro Ala Pro Ala Gly Gly
435 440 445
Leu Gly Gly Thr Gly Gly Ser Ala Val Ser Leu Ile Gly Leu Ala Ala
450 455 460
Glu Gly Glu Thr Glu Asp Phe Gly Glu Met Thr Gly Ala Ser Pro Ala
465 470 475 480
Asp Ala Ala Ala Val Asp Ala Glu Gly Lys Leu Gly Gly Gly Ala Arg
485 490 495
Pro Ser Ile Ile Arg Leu Ser Ala Thr Ala Gly Gly Ala Ala Pro Ala
500 505 510
Ala Ser Pro Arg Arg Val Gln Phe Ala Asp Asp Ser Thr Val Ile Asp
515 520 525
Val Arg Ser Ser Asp Gly Gly Ala Val Gly Ala Arg Pro Val Val Ile
530 535 540
Ser Ala Ala Ala Pro Gly Gly Leu Asp Tyr Arg Arg Gln Pro Leu Leu
545 550 555 560
Leu Glu Val Glu Leu Ala Ser Gly Ala Ser Pro Leu Ile Ser Pro Arg
565 570 575
Asp Ser Pro Ser Ser Ser Tyr Ser Ser Trp Leu His Asp
580 585
<210> 95
<211> 490
<212> PRT
<213> Micractinium conductrix
<400> 95
Met Pro Ala Ala Leu Cys Thr Gly Ser Ala Met Gln Leu Gln Arg Arg
1 5 10 15
Ser Pro Val Thr Ala Ala Ala Pro Ala Ala Ala Ala Gln Arg Leu Val
20 25 30
Ala Pro Ala Ala Ala Ala Ala Pro Gln His Ala Ala Ser Leu Arg Cys
35 40 45
Ser Leu Arg Ser Thr Phe Pro Gly Gly Ala Ser Ile Ser Ser Val Ala
50 55 60
Ala Leu Arg Thr Ser Ala Gly Arg Arg Ser Leu His Val Ala Gly Ala
65 70 75 80
Val Gly Pro Asn Glu Lys Arg Ala Gln Ile Gln Gln Gln Arg Leu Asp
85 90 95
Ser Leu Ser Lys Ile Asp Val Leu Phe Asp Glu Glu Leu Lys Ala Leu
100 105 110
Asp Thr Asn Leu Val Lys Glu Leu Val Ala Asp Asp Phe Lys Glu Gln
115 120 125
Asp Arg Lys Lys Leu Arg Val Val Phe Asp Phe Asp Arg Trp Gln Arg
130 135 140
His Arg Ser Ser Ser Arg Tyr Leu Arg His Met Thr Gly Leu Phe Thr
145 150 155 160
Ser Arg Thr Leu Lys Trp Met Gly Ala Pro Leu Val Cys Thr Leu Ser
165 170 175
Val Ala Thr Ala Val Gly Ile Tyr Tyr Thr Ala Ala Glu Met Gly Met
180 185 190
Val Pro Asp Ile Pro Asp Ile Lys Thr Asn Ala Val Phe Gly Leu Thr
195 200 205
Ser Phe Ala Leu Ala Asn Leu Leu Val Leu Thr Thr Asn Thr Ala Tyr
210 215 220
Gln Arg Phe Asp Glu Ala Arg Lys Met Trp Gly Leu Ile Val Asn Arg
225 230 235 240
Ser Arg Asp Met Thr Arg Gln Gly Leu Ala Tyr Ile Pro Glu Asp Gln
245 250 255
Pro Glu Leu Gln Glu Met Phe Cys Arg Trp Val Val Ala Tyr Ser Arg
260 265 270
Ser Leu Met Cys His Leu Arg Ala Gly Glu Asn Leu Glu Arg Glu Leu
275 280 285
Leu Gly Lys Leu Pro Glu Ser Glu Leu Arg Ala Leu Leu Thr Ala Thr
290 295 300
His Arg Pro Asn Phe Thr Asn Gln Val Ile Ala Gln Ile Leu Lys Arg
305 310 315 320
Ala Asp Leu Pro Gly Ala Glu Val Lys Pro Trp Asp Ser Thr Ala Asn
325 330 335
Val Lys Ala Ser Ala Phe Val Arg Met Asp Glu His Leu Thr Val Phe
340 345 350
Ala Asp Val Thr Gly Gly Cys Glu Arg Ile Leu Arg Thr Pro Val Pro
355 360 365
Leu Met Tyr Ser Arg His Thr Ser Arg Met Leu Thr Ile Trp Leu Ser
370 375 380
Phe Leu Pro Phe Ser Leu Trp Asp Ala Cys His Trp Trp Thr Val Pro
385 390 395 400
Val Thr Gly Leu Val Ala Tyr Leu Leu Leu Gly Ile Lys Glu Ile Gly
405 410 415
Leu Thr Val Glu Glu Pro Phe Ser Ile Leu Pro Leu Glu Lys Ile Cys
420 425 430
Asp Thr Ile Glu Ser Asn Val Trp Glu Leu His Ser Thr His Ser Ala
435 440 445
Gln Gly Ala Ala Ala Ser Arg Arg Arg Ser Ala Ala Thr Arg Asp Ala
450 455 460
Thr Gln Ser Met Asp Ala Glu Asp Leu Val Ser Ile Ala Ala Ala Val
465 470 475 480
Asp Ala Gly Ala Met Pro Ala Pro Ala Gln
485 490
<210> 96
<211> 460
<212> PRT
<213> Micractinium conductrix
<400> 96
Met Arg Leu Leu Pro Gly Cys Arg Arg Pro Gly Ala Ala His Gly Thr
1 5 10 15
Ala Ala Ser Leu Gly Cys Arg Pro Gln Arg Gln Arg Leu Arg Arg Ala
20 25 30
Pro Leu Ala Thr Ala Gln Pro Ser Asp Gly Asp Ser Ser Ser Ser Ser
35 40 45
Asn Gly Thr Gly Leu Leu Ser Lys Val Pro Ala Val Leu Lys Ser Val
50 55 60
Asp Glu Asp Ala Val Tyr Val Tyr Asp Ser Gly Ser Asp Asp Val Pro
65 70 75 80
Leu Asp Ile Leu Leu Leu Pro Leu Asp Arg Asp Asp Tyr Lys Glu Asp
85 90 95
Ser Arg Arg His Phe Arg Thr Val Phe Asp His Asn Arg Trp Ala Ala
100 105 110
His Arg Ser Thr Arg Arg Tyr Val Arg His Val Leu Gly Met Thr Ser
115 120 125
Ser Arg Met Val Arg Gly Leu Ala Ala Pro Leu Phe Phe Val Ala Gly
130 135 140
Ile Ser Ala Ala Val Cys Ala Gly His Gln Ala Ala Glu Ala Gly Leu
145 150 155 160
Leu Pro Leu Pro Glu Val Ala His Gln Leu Gln Met Arg Ser Thr Glu
165 170 175
Pro Phe Ala Leu Thr Ser Phe Ala Leu Ser Leu Leu Leu Val Phe Arg
180 185 190
Thr Asn Ser Ser Tyr Ser Arg Trp Leu Asp Ala Arg Lys Asn Trp Gly
195 200 205
Leu Val Val Thr Arg Ser Arg Asp Leu Val Arg Gln Gly Ile Thr His
210 215 220
Ile Pro His Ser Glu Arg Ala Leu Ile Asp Leu Leu Cys Arg Trp Thr
225 230 235 240
Ala Ala Phe Ser Arg Ser Leu Met Ala His Leu Arg Ala Asp Val Asp
245 250 255
Ala Glu Ala Glu Leu Arg Arg Val Leu Arg Pro Ala Glu Ala Glu Ala
260 265 270
Ala Leu Ala Ala Glu His Arg Pro Gly Tyr Cys Leu Gln Val Leu Ser
275 280 285
Glu Ile Ile Lys Asp Ala Gln Leu Glu Arg Pro His Ala Ala Ala Thr
290 295 300
Ala Ala Ala Ala Thr Ala Arg Pro Gly Gln Ala Ala Lys Pro Thr Arg
305 310 315 320
Leu Asp Gly Gly Ala Ala Cys Arg Met Asp Glu Asn Leu Thr Ala Leu
325 330 335
Glu Asp Met Val Gly Gly Cys Glu Arg Ile Leu Arg Thr Pro Ile Pro
340 345 350
Leu Ser Tyr Thr Arg His Thr Ser Arg Phe Met Met Ile Phe Leu Ser
355 360 365
Leu Leu Pro Phe Ala Leu Trp Glu Ser Cys Arg Trp Ala Thr Val Pro
370 375 380
Ala Val Thr Ile Ile Ser Phe Leu Leu Leu Gly Ile Glu Glu Ile Gly
385 390 395 400
Val Thr Ile Glu Glu Pro Phe Ser Ile Leu Pro Leu Glu Gln Leu Cys
405 410 415
Asp Val Ile Glu Lys Asn Val Trp Glu Leu His Arg Cys His Val Gln
420 425 430
Pro Ala Ser Ala Ala Ile Asp Gly Ser Gly Ser Arg Pro Leu Thr Ala
435 440 445
Ala Gly Val Val Ala Ala Ala Arg Leu Ala Glu Asp
450 455 460
<210> 97
<211> 511
<212> PRT
<213> Micractinium conductrix
<400> 97
Met Arg Thr Glu Pro Gln Thr Arg Leu Ala Thr Gly Gly Ala Ala Thr
1 5 10 15
Gln Arg Arg Arg Ser Ala Pro Val Val Ala Gly Ser Ala Ala Gly Leu
20 25 30
Pro Pro Pro Ser Pro Phe Ala Ser Ser Arg Ser Gly Ala Arg Thr Gln
35 40 45
Arg Gly Ala Ala Ser Pro Glu Gln Arg Glu Val Thr Trp Ser Leu Glu
50 55 60
Asp Ala Gly Ala Asp Met Thr Ile Asn Pro Asp Arg Glu Ala Gly Arg
65 70 75 80
Arg Leu Met Arg Asp Leu Ser Asn Phe Ser His Arg Arg Trp Ala Phe
85 90 95
His Arg Ser Thr Asp Arg Tyr Trp Arg His Leu Ala Gly Ile Phe Gln
100 105 110
Ser Arg Ile Gly Arg Gln Leu Phe Val Pro Leu Ser Cys Ala Gly Gly
115 120 125
Thr Ala Ala Ala Ile Cys Ala Tyr Glu Thr Leu Leu Arg Asp Gly Ala
130 135 140
Leu Pro Ala Tyr Phe Pro Ser Phe Val Leu Pro Ser Leu Pro Phe Asp
145 150 155 160
Phe Thr Ala Phe Ala Leu Ser Leu Leu Leu Val Phe Arg Thr Asn Thr
165 170 175
Ser Tyr Asp Arg Trp Gln Ala Ala Ile Asn Ala Trp Gly Asp Ile Ser
180 185 190
Thr Arg Ser Arg Asp Thr Leu Arg Gln Leu Leu Ala Tyr Thr Ser Ala
195 200 205
Thr Pro Ala Ala Ala Ala Val Ala Arg Asp Thr Ser Ala Ala Pro Leu
210 215 220
His Gly Ala Gly Gly Ser Gly Ala Ala Ala Ala Ser Ala Ala Gln Thr
225 230 235 240
Thr Ala Ala Ala Gly Lys Trp Leu Val Ala Phe Ser Arg Ser Leu Lys
245 250 255
Ala Gln Leu Thr Glu Asp Ser Asp Leu Gln Ala Glu Leu Lys Gly Val
260 265 270
Leu Thr Ala Gly Glu Leu Glu Leu Leu Thr Ala Ala Tyr His Arg Pro
275 280 285
Ser Phe Ala Leu Ala Val Leu Ser Glu Leu Val Ala Ala Ala Pro Val
290 295 300
Arg Asp Ser Gln Arg Ala Arg Leu Asp Glu Asn Leu Thr Thr Leu Glu
305 310 315 320
Asp Met Val Gly Gly Cys Glu Arg Leu Leu Arg Thr Pro Ile Pro Leu
325 330 335
Ser Tyr Thr Arg His Thr Ser Arg Phe Met Val Ile Trp Leu Ser Cys
340 345 350
Leu Pro Leu Cys Leu Trp Asn Ser Cys Gly Trp Gly Thr Val Pro Leu
355 360 365
Thr Val Val Ile Ser Phe Leu Leu Leu Gly Ile Glu Glu Ile Gly Val
370 375 380
Ala Ile Glu Glu Pro Phe Ser Ile Met Pro Leu His Glu Met Cys Ala
385 390 395 400
Glu Met Glu Cys Gly Leu Ala Asp Ile Leu Glu Gln Ala Ala Pro Ser
405 410 415
Lys Arg Ala Ala Ala Gln Ala Ala Ala Ala Ala Ala Leu Thr Ala Ala
420 425 430
Ser Ala Ala Val Gly Gly Ser Ala Asp Ala Pro Ala Asp Ala Ser Gly
435 440 445
Gly Gly Gly Gly Gly Gly Ser Thr Ala Gly Ala Ala Pro Ala Pro Ala
450 455 460
Leu Gly Val Pro Ala Ala Ala Ala Asn Ser Val Ala Pro Phe Val Leu
465 470 475 480
Gly Met Thr Ala Gln Arg Leu Arg Arg Pro Leu Ser Ala Thr Pro Arg
485 490 495
Pro Met Asp Ala Ile Phe Cys Ser Met Asp Asp Pro Asp Ala Arg
500 505 510
<210> 98
<211> 523
<212> PRT
<213> Micractinium conductrix
<400> 98
Met Pro Arg Thr Glu Asn Ser Tyr Thr His Gly Ala Arg Gly Ser Pro
1 5 10 15
Ser Leu Asp Pro Glu Gly Ser Pro Leu Thr Gln His Ala Arg Asn Ser
20 25 30
Ser Leu Gln Asn Leu Ala Arg Leu Pro Gly Val Ala Arg Ala Lys Leu
35 40 45
Gln Asn Leu Ser Gln Thr Lys Phe Val Arg Tyr Ile Glu Asp Asn Asp
50 55 60
Asp Gly Ser Arg Asp Glu Asn Asp Ile Tyr Arg Gln His Pro Gly Asp
65 70 75 80
Gln Val Phe Thr Phe Glu Arg Trp Asn Lys His Arg Asp Ser Phe Arg
85 90 95
Tyr Ala Lys His Leu Ala His Met Phe Ser Ser Arg Val Phe Lys Gln
100 105 110
Leu Leu Gly Pro Val Leu Cys Val Ser Ala Leu Ala Leu Ala Val Gly
115 120 125
Val Tyr Glu Thr Leu Val Ala Ala Gly Lys Leu Pro Gly His Trp Pro
130 135 140
His Val Thr Leu Ala Leu Gly Gln Gly Phe Asn Leu Thr Ala Phe Ala
145 150 155 160
Leu Ser Leu Leu Leu Val Phe Arg Thr Asn Ser Ser Tyr Asp Arg Trp
165 170 175
Trp Glu Ala Arg Lys Leu Trp Gly Gly Val Val Asn Arg Thr Arg Asp
180 185 190
Phe Val Arg Gln Ser Leu Val Phe Phe Arg Asp Glu Asp Ala His Leu
195 200 205
Arg Glu Leu Leu Ala Lys Trp Thr Ile Ala Phe Pro His Val Leu Met
210 215 220
Cys His Leu Arg Glu His Gly Asp Val Ala Lys Ala Val Ala Met Met
225 230 235 240
Ala Glu Ile Val Arg Thr Ala Arg Pro Pro Glu Leu Cys Arg Met Arg
245 250 255
Met Asp Glu Asn Leu Ser Phe Phe Glu Asp Ala Met Gly Ser Met Glu
260 265 270
Arg Ile Leu Arg Thr Pro Ile Pro Leu Ser Leu Val Trp Leu Ile Leu
275 280 285
Leu Pro Phe Thr Leu Trp Ala Ala Tyr Ser Trp Phe Ser Val Leu Leu
290 295 300
Ser Gly Ile Phe Ala Phe Leu Met Phe Gly Ile Asp Glu Ile Gly Val
305 310 315 320
Gln Ile Glu Glu Pro Phe Gly Ile Leu Pro Leu Glu Ala Ile Ser Ser
325 330 335
Thr Ile Glu Arg Asn Ile Arg Glu Leu Leu Ala Arg Asn Asp Glu Val
340 345 350
Val Gly Leu Val Ala Gln Ala Ala Gln Ala Gly Ala Val Ala Ala Ala
355 360 365
Ala Ala Ala Ala Ala Asp Val Ala Val Ala Ala Leu Pro Gln Gly Gly
370 375 380
Gln His Asp Ala Asp Ala Ala Thr Val Leu Ala Val Pro Gly Asp Glu
385 390 395 400
Phe Gly Glu Met Val Ala Ala Gly Ser Pro Val Gly Lys Glu Pro Ser
405 410 415
Ala Gly Asn Leu Ile Thr Leu Thr Ala Ser Ser Gly Glu Ala Ala Ala
420 425 430
Ala Ala Ala Ala Ala His His His Leu His Ala Ala Ala Arg Arg Val
435 440 445
Gln Phe Gly Gly Ala Thr Val Ile Glu Val Gly Gly Ser Asp Glu Glu
450 455 460
Ala Ala Gly Gly Gly Gly Gly Ser Pro Leu Gly Ser Pro Ser His Ala
465 470 475 480
His Ser Pro His Arg Ala Leu Asn Arg Gln Ala Ser Met Val Glu Val
485 490 495
Glu Leu Ala Ser Gly Ala Ser Pro Leu Leu Ser Pro Thr Asp Gly Ser
500 505 510
Ser Ala Pro Val Tyr Ser Asn Trp Leu Arg Asp
515 520
<210> 99
<211> 425
<212> PRT
<213> Micractinium conductrix
<400> 99
Met Thr Leu Leu Gln Gln Ala Val Pro Ser Arg Val Val Val Gly Gly
1 5 10 15
Arg Gln Pro Arg Gly Ile Gly Ser Val Leu Lys Pro Leu Arg Thr Ser
20 25 30
Cys Arg Pro Ala Lys Ala Ala Arg Gly Arg Gln Ala Leu Pro Gln Ala
35 40 45
Ser Leu Gln Pro Ala Val Ala Ala Gly Thr Ala Ala Pro Pro Pro Pro
50 55 60
Ser Tyr Val Ala Ser Ala Asn Asp Glu Arg Met Glu Ala Ser Arg Val
65 70 75 80
Glu Met Arg Ala Val Phe Asp Ala Asp Arg Trp Ile Met His Arg Ser
85 90 95
Thr Asp Arg Tyr Phe Arg His Leu Trp Gly Leu Pro Ser Ser Arg Val
100 105 110
Leu Gln Gly Leu Phe Lys Pro Ile Gly Tyr Ile Leu Ala Ile Ser Ala
115 120 125
Ala Val Cys Ala Tyr Gly Thr Ala Gln Ala Ala Gly Ala Leu Pro Ala
130 135 140
Thr Leu Pro Leu Leu Ala Ser Ala Ile Lys Leu Lys Glu Leu Phe Gly
145 150 155 160
Met Thr Ser Phe Ala Leu Ser Leu Leu Leu Val Phe Arg Thr Asn Ala
165 170 175
Ser Tyr Ala Arg Trp Asp Glu Gly Arg Lys Met Trp Gly Met Val Leu
180 185 190
Asn Arg Thr Arg Asn Ile Cys Arg Leu Gly Leu Ala Trp Ile Gly Asp
195 200 205
Asp Lys Arg Glu Leu Arg Ser Met Leu Cys Arg Trp Ala Pro Ala Phe
210 215 220
Ser Lys Thr Leu Met Cys His Leu Arg Lys Gly Glu Asp Leu Arg Glu
225 230 235 240
Glu Leu Glu Gly Ile Leu Leu Pro His Glu Ile Glu Gly Val Leu Arg
245 250 255
Ala Ser His Arg Pro Asn Tyr Val Leu Gln Val Leu Ala Gln Ile Val
260 265 270
Arg Thr Ala Gly Leu Pro Thr Ala Ala Thr Leu Arg Met Glu Asp Asp
275 280 285
Leu Thr Asn Phe Gly Asp Ser Leu Gly Gly Cys Glu Arg Leu Leu Arg
290 295 300
Thr Pro Ile Pro Leu Phe Tyr Thr Arg His Thr Ser Arg Phe Leu Met
305 310 315 320
Ile Trp Leu Thr Phe Leu Pro Ala Thr Leu Trp Pro Ala Cys Gly Leu
325 330 335
Leu Thr Leu Pro Leu Val Phe Leu Ile Ser Phe Leu Leu Leu Gly Val
340 345 350
Asp Lys Ile Gly Val Ser Ile Glu Glu Pro Phe Ser Ile Leu Ala Leu
355 360 365
Glu Val Ile Ala Gly Thr Ala Leu Ala Asn Val Arg Glu Leu Val Ala
370 375 380
Met His Glu Asp Thr Ala Ala Gly Val Ala Ala Ala Ala Asn Gly Asn
385 390 395 400
Gly Gly Ala Ala Gly Ala Pro Ala Glu Ala Asp Val Ser Ala Ala His
405 410 415
Met Val Thr Leu Ala Gly Ala Ala Ala
420 425
<210> 100
<211> 490
<212> PRT
<213> Micractinium conductrix
<400> 100
Met Ser Ala Leu Leu Arg Ala Arg Val Ser Ser Phe Leu Ala Gly Val
1 5 10 15
Ala Val Ala Gly Val Phe Gly Val Tyr Gln Leu Arg Gly Asp Val Ala
20 25 30
Glu Gly Gln Ala Gln Leu Leu Glu Gln Thr Lys Lys Tyr Thr Ser Gly
35 40 45
Leu Glu Ala Arg Val Ala Glu Leu Glu Ala Ser Val Ala Arg Leu Gln
50 55 60
Lys Ser Ser Leu Leu Leu Ala Ser Arg Pro Leu Gly Ala Gly Leu Val
65 70 75 80
Gly Leu Arg Ser Ser Gln Gly Gly Leu Glu Arg Arg Arg Arg Ala Ser
85 90 95
Ala Arg Val Ala Ala Val Ala Ser Pro Val Gly Asp Ile Glu Gly Arg
100 105 110
Lys Pro Thr Val Asp Glu Arg Arg Glu Ser Asp Leu Lys Glu Leu Arg
115 120 125
Ser Val Phe Asp Phe Ala Ala Trp Lys Arg His Arg Ser Ser Asp Arg
130 135 140
Tyr Trp Arg His Met Thr Gly Leu Trp Asp Ser Arg Thr Leu Asp Trp
145 150 155 160
Val Gly Pro Pro Leu Thr Tyr Val Met Ser Val Ala Ala Leu Val Cys
165 170 175
Leu Tyr His Ala Ala Ala Asp Ala Gly Tyr Val Ala Glu Val Ile Pro
180 185 190
Glu Leu Lys Pro Ala Ala Thr Ala Pro Phe Ser Leu Thr Ser Phe Ala
195 200 205
Leu Ser Thr Leu Leu Val Leu Arg Thr Asn Thr Ser Tyr Gln Arg Trp
210 215 220
Asp Glu Ala Arg Lys Met Trp Gly Leu Ile Val Asn Arg Ser Arg Asp
225 230 235 240
Ile Ser Arg Gln Ala Leu Gly Tyr Ile Pro Ala Thr Gln Pro Glu Leu
245 250 255
Gln Asp Met Phe Ile Arg Trp Val Val Ala Tyr Ser Arg Ser Leu Met
260 265 270
Cys His Leu Arg Ala Gly Glu Asp Leu Glu Lys Glu Leu Lys Asp Thr
275 280 285
Leu Gly Pro Glu Glu Leu Lys Ala Leu Met Ala Ser Ala His Arg Pro
290 295 300
Asn Tyr Thr Val Gln Val Leu Thr Ser Ile Ile Lys Ala Ala Gln Leu
305 310 315 320
Pro Gly Glu Pro Ile Ser Asn Tyr Asn Ser Thr Ala Asn Val Lys Ala
325 330 335
Ser Ala Ala Phe Arg Met Asp Glu Asn Leu Thr Cys Phe Ala Asp Val
340 345 350
Thr Gly Gly Cys Glu Arg Ile Leu Arg Thr Pro Val Pro Leu Met Tyr
355 360 365
Thr Arg His Asn Ser Arg Phe Leu Met Ile Trp Leu Thr Leu Leu Pro
370 375 380
Leu Thr Leu Trp Asp Ser Cys His Trp Phe Thr Met Pro Val Thr Gly
385 390 395 400
Ile Val Ala Phe Leu Leu Leu Gly Ile Lys Glu Ile Gly Val Ile Val
405 410 415
Glu Glu Pro Phe Ser Ile Leu Pro Leu Glu Lys Ile Cys Asp Thr Ile
420 425 430
Glu Gly Asn Val Arg Glu Leu His Ala Thr His Ser Ala Asp Ala Ile
435 440 445
Ser Ala Gln Leu Ala Val Ala Asn Ala Gln Asn Ser Gly Ala Ala Ser
450 455 460
Gly Gly Val Asp Ala Asp Thr Leu Val Asn Thr Val Val Pro Ala Val
465 470 475 480
Thr Leu Arg Lys Pro Ala Val Ala Ala Leu
485 490
<210> 101
<211> 1322
<212> PRT
<213> Micractinium conductrix
<400> 101
Met Gly Ala Ser Thr Asp Asp Ser Ala Ala Met Ala Asp His Pro Gly
1 5 10 15
Arg Thr Pro Gly Gly Arg Pro Pro Pro Leu Ser Ser Ser Val Gly Gly
20 25 30
Ser Ser Arg Ala Lys Ala Met Leu Ala Met Ala Thr Gly Gly Gly Gly
35 40 45
Lys Ala Lys Thr Arg Thr Pro Ser Gly Arg Pro Thr Pro Pro Pro Ile
50 55 60
Thr Phe Glu Arg Arg Lys Lys Arg Pro Gln Ser Glu Pro Gly Asp Gly
65 70 75 80
Gly Gly Gly Glu Ala Ala Ala Leu Gly Gly Glu Thr Thr Pro Lys Arg
85 90 95
Gln Arg Thr Pro Ala Gly Ala Asp Cys Pro Ser Val Pro Val Ala Ala
100 105 110
Pro Arg Pro Ala Ser Leu Leu Ala Ala Arg Pro Gly Ser Ala Ala Gly
115 120 125
Ser Ala Gly Asp Ala Pro Arg Leu Thr Cys Tyr Ser Asn Ala Gln Arg
130 135 140
Leu Arg Glu Leu Ala Ala Glu Gly Gly Asp Ala Leu Ala Arg Arg Arg
145 150 155 160
Ala Ala Leu Gly Gly Ser Gly Phe Ile His Arg Arg Pro Ser Ser Gly
165 170 175
Gly Ser Leu Gly Arg Met Gln Gly Leu Ser Arg Leu Pro Ala Gly Met
180 185 190
Met Asn Leu Gly Asn Thr Cys Tyr Leu Asn Ala Val Leu Gln Val Leu
195 200 205
Leu Ser Leu Pro Ser Phe Val Ala Asp Leu Arg His Gly Arg Ala Ala
210 215 220
Leu Glu Ala Ala Gly Gln Pro Leu Ala Pro Ser Gly Val Tyr Ser Ala
225 230 235 240
Leu Leu Glu Cys Ala Ala Ala Arg Glu Arg Gly Phe Glu Arg Tyr Leu
245 250 255
Thr Pro Ala Ser Leu Lys Arg Ala Leu Asp Ala Ala Ser Ser Ala Phe
260 265 270
His Gly Thr Leu Gln Gln Asp Ala His Glu Leu Phe Cys Ser Leu Leu
275 280 285
Asp Ala Ala Gln Gly Glu Val Leu Ala Arg Glu Val Ala Arg Leu Gly
290 295 300
Arg Thr Arg Val Ala Val Ser Glu Thr Ala Asp Pro Gly Ala Arg Asn
305 310 315 320
Phe Gly Phe Ala Met Gln His Glu Leu Val Cys Gly Ala Cys Gly His
325 330 335
Thr Ser Gln Leu Val Glu Gln Tyr Thr His Leu Ser Leu Glu Leu Pro
340 345 350
Pro Ala Gln Pro Gly Gly Thr Ala Ala Cys Ser Val Ala Ser Leu Leu
355 360 365
Arg Asp Tyr Phe Lys Glu Glu Gln Val Glu Lys Ala Cys Glu Ala Cys
370 375 380
Gly Gly Gly Gly Val Ala His Thr Leu Arg His Ala Val Lys Arg Leu
385 390 395 400
Pro Arg Val Leu Val Leu His Leu Lys Arg Phe Lys Ile Ser Trp Ser
405 410 415
Ala Glu Gln Gln Arg Val Val Cys Gln Lys Val His Thr Gly Val Gly
420 425 430
Met Pro Glu Thr Val Gln Leu Gly Arg Leu Leu Ala Ala Gly Pro Ala
435 440 445
Pro Pro Leu Leu Gly Gly Gln Gln Leu Gly Ala Gly Lys Glu Asn Glu
450 455 460
Gln Leu Glu Ala Asn Ala Gly Val Val Gly Ser Ala Ala Gln Gln Gln
465 470 475 480
Gln Gln Gln Gln Gln Arg Gly Asp Asp Ser Val Ala Lys Gly Leu Arg
485 490 495
Met Arg Glu Gln Ala Gly Phe Ser Phe Tyr Ser Gly Gly Gly Gly Ala
500 505 510
Thr Pro Val Ala Ala Ser Ala Leu Leu Ser Pro Gly Ala Leu His Ser
515 520 525
Pro Thr Leu Arg Arg Arg Leu Gly Thr Pro Ala Ala Pro Lys Leu Ser
530 535 540
Asn Ile Val Val Asp Asn Asp Asp Ala Val Leu Gln Arg Val Leu Ala
545 550 555 560
Glu Ser Leu Arg Glu Gln Gln Pro Gly Gly Gly Gln Leu Pro Gly Gly
565 570 575
Thr Ala Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln
580 585 590
Gln Gln Gln Asp Gly Pro Glu Leu Ser Leu Gly Pro Gly Gly Cys Leu
595 600 605
Gln Arg His Ser Thr Glu Gly Ala Glu Cys Pro Leu Ile Arg Cys Gly
610 615 620
Ser Pro Gly Leu Arg Gln Ala Glu Ala Ala Pro Leu Leu Leu Pro Glu
625 630 635 640
Val Ala Ala Ala Ala Ala Ala Gly Ser Ala Pro Ala Gly Gly Cys His
645 650 655
Lys Leu Glu Asp Gln Trp Phe Thr Gly Phe Arg Arg Gly Ser Pro Pro
660 665 670
Ala Ala Pro Met Ala Ala Glu Gln Glu Ala Ala Glu Leu Gln Arg Val
675 680 685
Leu Asp Met Ser Arg Arg Glu His Glu Arg Gln Ala Ala Ala Val Ala
690 695 700
Val Arg Ser Ser Ser Ala Ala Gly Ser Ala Ala Gln Gln Glu Glu Ala
705 710 715 720
Asp Leu Leu Arg Ala Ile Glu Leu Ser Met Gln Glu Gln Arg Gly Lys
725 730 735
Gln Pro Thr Ser Thr Gln Pro Gln Gln His Gly Gly Ser Gln Gly Thr
740 745 750
Ala Gly Gly Asp Ser Ser Gly Gly Ala Gly Gly Ala Ala Ala Gly Ala
755 760 765
Ala Ala Pro Gly Ala Ser Ala Ala Ala Ala Ala Ala Ala Ala Ala Val
770 775 780
Thr Glu Gln Phe Asp Ala Gly Glu Ala Gly Lys Ala Glu Glu Ala Ala
785 790 795 800
Ser Ala Val Gln Ser Val Ala Pro His Gly Asp Asp Gly Val Glu Leu
805 810 815
Val Ala Asp Ala Gly Gly Ala Ala Pro Ala Pro Leu His Ala Gln Ile
820 825 830
Val Glu Asp Asn Asp Ala Gln Leu Ala Ala Gln Arg Ala Gln Gln Ala
835 840 845
Gln His Gly Ser Ser Gly Ala Ser Ala Asp Gln Gln Ala Ala Ala Glu
850 855 860
Val Thr Asp Ala Ala Glu Ala Ala Ala Gly Ala Glu Glu Lys Ala Ala
865 870 875 880
Ala Ala Ala Ala Val Arg Ser Gly Lys Pro Pro Ala Ala Glu Tyr Arg
885 890 895
Leu His Ala Val Val Asn His Lys Gly Pro Ala Ala Ser Cys Gly His
900 905 910
Phe Thr Ser Asp Ile Arg Asp Ala Ala Ser Gly Val Trp His Arg Phe
915 920 925
Asp Asp Ser Leu Ala Ser Arg Ile Ala Gly Trp Glu Ala Arg Ser Ser
930 935 940
Glu Lys Gln Arg Glu Cys Tyr Leu Leu Phe Tyr Thr Gly Thr Val Ala
945 950 955 960
Gly Leu Ile Tyr Ser Asp Arg Asp Glu Gly Lys Glu Phe Arg Leu Ala
965 970 975
Asp Phe Arg Thr Val Tyr Thr Phe Glu Ser Trp Ala Ala His Arg Ser
980 985 990
Thr Met Arg Tyr Leu Arg His Phe Met Gly Met Phe Arg Ser Arg Val
995 1000 1005
Leu Cys Gly Leu Arg Val Pro Leu Leu Leu Ile Ala Leu Glu Ala Thr
1010 1015 1020
Leu Val Cys Ser Tyr Glu His Ala Leu Lys Asn Gly Leu Leu Pro Ala
1025 1030 1035 1040
Phe Cys Arg Thr Ile Gln Ile Arg Ala Pro Met Leu Phe Ser Leu Ser
1045 1050 1055
Ser Phe Ala Leu Ser Leu Leu Leu Val Phe Arg Thr Asn Gln Ser Tyr
1060 1065 1070
Ala Arg Phe Asp Glu Gly Arg Arg Leu Trp Ser Val Met Ala Asn Arg
1075 1080 1085
Cys Arg Asp Val Met Arg Gln Ala Cys Ser Tyr Leu Ser Leu Gly Pro
1090 1095 1100
Gln Asn Ala Ala Leu Leu Ala Thr Leu Ser Arg Trp Met Gln Ala Phe
1105 1110 1115 1120
Pro Trp Val Met Lys Asn Tyr Val Arg Glu Ser Lys Ser Trp His Ala
1125 1130 1135
Asp Leu Gly Ser Leu Leu Arg Pro Ala Glu Leu Ala Ala Leu Leu Asp
1140 1145 1150
Thr Arg Asn Pro Pro Leu Leu Thr Leu Gln Val Ile Thr Glu Ile Leu
1155 1160 1165
Asp Arg Met Pro Ile Ser Thr Lys Gln Arg Leu Arg Leu Asp Ile Phe
1170 1175 1180
Met Thr Glu Leu Ser Asp Met Val Gly Ala Ser Glu Arg Leu Ile Thr
1185 1190 1195 1200
Thr Pro Ile Pro Leu Ser Tyr Thr Arg His Thr Ala Arg Phe Leu Ile
1205 1210 1215
Thr Trp Leu Val Leu Thr Pro Phe Thr Ile Phe Ser Ser Cys Gly Trp
1220 1225 1230
Val Thr Ile Phe Ser Ser Val Leu Leu Ala Phe Phe Leu Leu Thr Ile
1235 1240 1245
Glu Glu Ile Gly Val Thr Ile Glu Glu Pro Phe Asn Ile Leu Pro Leu
1250 1255 1260
Glu Ala Ile Asn Thr Arg Val Cys Ala Asp Ile Ala Arg Tyr Pro Gln
1265 1270 1275 1280
Lys Ile Asp Thr Val Val Ser Leu Val Asp Asp Ala Thr Gly Thr Gln
1285 1290 1295
Arg Pro Gln Gln Gln Pro Pro Phe Gln Pro Val Ala Glu Phe Val Ser
1300 1305 1310
Thr Ala Ala Thr Ser Asp Asn Gly Asp Ser
1315 1320
<210> 102
<211> 1327
<212> PRT
<213> Micractinium conductrix
<400> 102
Met Gly Ala Ser Thr Asp Asp Ser Ala Ala Met Ala Asp His Pro Gly
1 5 10 15
Arg Thr Pro Gly Gly Arg Pro Pro Pro Leu Ser Ser Ser Val Gly Gly
20 25 30
Ser Ser Arg Ala Lys Ala Met Leu Ala Met Ala Thr Gly Gly Gly Gly
35 40 45
Lys Ala Lys Thr Arg Thr Pro Ser Gly Arg Pro Thr Pro Pro Pro Ile
50 55 60
Thr Phe Glu Arg Arg Lys Lys Arg Pro Gln Ser Glu Pro Gly Asp Gly
65 70 75 80
Gly Gly Gly Glu Ala Ala Ala Leu Gly Gly Glu Thr Thr Pro Lys Arg
85 90 95
Gln Arg Thr Pro Ala Gly Ala Asp Cys Pro Ser Val Pro Val Ala Ala
100 105 110
Pro Arg Pro Ala Ser Leu Leu Ala Ala Arg Pro Gly Ser Ala Ala Gly
115 120 125
Ser Ala Gly Asp Ala Pro Arg Leu Thr Cys Tyr Ser Asn Ala Gln Arg
130 135 140
Leu Arg Glu Leu Ala Ala Glu Gly Gly Asp Ala Leu Ala Arg Arg Arg
145 150 155 160
Ala Ala Leu Gly Gly Ser Gly Phe Ile His Arg Arg Pro Ser Ser Gly
165 170 175
Gly Ser Leu Gly Arg Met Gln Gly Leu Ser Arg Leu Pro Ala Gly Met
180 185 190
Met Asn Leu Gly Asn Thr Cys Tyr Leu Asn Ala Val Leu Gln Val Leu
195 200 205
Leu Ser Leu Pro Ser Phe Val Ala Asp Leu Arg His Gly Arg Ala Ala
210 215 220
Leu Glu Ala Ala Gly Gln Pro Leu Ala Pro Ser Gly Val Tyr Ser Ala
225 230 235 240
Leu Leu Glu Cys Ala Ala Ala Arg Glu Arg Gly Phe Glu Arg Tyr Leu
245 250 255
Thr Pro Ala Ser Leu Lys Arg Ala Leu Asp Ala Ala Ser Ser Ala Phe
260 265 270
His Gly Thr Leu Gln Gln Asp Ala His Glu Leu Phe Cys Ser Leu Leu
275 280 285
Asp Ala Ala Gln Gly Glu Val Leu Ala Arg Glu Val Ala Arg Leu Gly
290 295 300
Arg Thr Arg Val Ala Val Ser Glu Thr Ala Asp Pro Gly Ala Arg Asn
305 310 315 320
Phe Gly Phe Ala Met Gln His Glu Leu Val Cys Gly Ala Cys Gly His
325 330 335
Thr Ser Gln Leu Val Glu Gln Tyr Thr His Leu Ser Leu Glu Leu Pro
340 345 350
Pro Ala Gln Pro Gly Gly Thr Ala Ala Cys Ser Val Ala Ser Leu Leu
355 360 365
Arg Asp Tyr Phe Lys Glu Glu Gln Val Glu Lys Ala Cys Glu Ala Cys
370 375 380
Gly Gly Gly Gly Val Ala His Thr Leu Arg His Ala Val Lys Arg Leu
385 390 395 400
Pro Arg Val Leu Val Leu His Leu Lys Arg Phe Lys Ile Ser Trp Ser
405 410 415
Ala Glu Gln Gln Arg Val Val Cys Gln Lys Val His Thr Gly Val Gly
420 425 430
Met Pro Glu Thr Val Gln Leu Gly Arg Leu Leu Ala Ala Gly Pro Ala
435 440 445
Pro Pro Leu Leu Gly Gly Gln Gln Leu Gly Ala Gly Lys Glu Asn Glu
450 455 460
Gln Leu Glu Ala Asn Ala Gly Val Val Gly Ser Ala Ala Gln Gln Gln
465 470 475 480
Gln Gln Gln Gln Gln Arg Gly Asp Asp Ser Val Ala Lys Gly Leu Arg
485 490 495
Met Arg Glu Gln Ala Gly Phe Ser Phe Tyr Ser Gly Gly Gly Gly Ala
500 505 510
Thr Pro Val Ala Ala Ser Ala Leu Leu Ser Pro Gly Ala Leu His Ser
515 520 525
Pro Thr Leu Arg Arg Arg Leu Gly Thr Pro Ala Ala Pro Lys Leu Ser
530 535 540
Asn Ile Val Val Asp Asn Asp Asp Ala Val Leu Gln Arg Val Leu Ala
545 550 555 560
Glu Ser Leu Arg Glu Gln Gln Pro Gly Gly Gly Gln Leu Pro Gly Gly
565 570 575
Thr Ala Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln
580 585 590
Gln Gln Gln Asp Gly Pro Glu Leu Ser Leu Gly Pro Gly Gly Cys Leu
595 600 605
Gln Arg His Ser Thr Glu Gly Ala Glu Cys Pro Leu Ile Arg Cys Gly
610 615 620
Ser Pro Gly Leu Arg Gln Ala Glu Ala Ala Pro Leu Leu Leu Pro Glu
625 630 635 640
Val Ala Ala Ala Ala Ala Ala Gly Ser Ala Pro Ala Gly Gly Cys His
645 650 655
Lys Leu Glu Asp Gln Trp Phe Thr Gly Phe Arg Arg Gly Ser Pro Pro
660 665 670
Ala Ala Pro Met Ala Ala Glu Gln Glu Ala Ala Glu Leu Gln Arg Val
675 680 685
Leu Asp Met Ser Arg Arg Glu His Glu Arg Gln Ala Ala Ala Val Ala
690 695 700
Val Arg Ser Ser Ser Ala Ala Gly Ser Ala Ala Gln Gln Glu Glu Ala
705 710 715 720
Asp Leu Leu Arg Ala Ile Glu Leu Ser Met Gln Glu Gln Arg Gly Lys
725 730 735
Gln Pro Thr Ser Thr Gln Pro Gln Gln His Gly Gly Ser Gln Gly Thr
740 745 750
Ala Gly Gly Asp Ser Ser Gly Gly Ala Gly Gly Ala Ala Ala Gly Ala
755 760 765
Ala Ala Pro Gly Ala Ser Ala Ala Ala Ala Ala Ala Ala Ala Ala Val
770 775 780
Thr Glu Gln Phe Asp Ala Gly Glu Ala Gly Lys Ala Glu Glu Ala Ala
785 790 795 800
Ser Ala Val Gln Ser Val Ala Pro His Gly Asp Asp Gly Val Glu Leu
805 810 815
Val Ala Asp Ala Gly Gly Ala Ala Pro Ala Pro Leu His Ala Gln Ile
820 825 830
Val Glu Asp Asn Asp Ala Gln Leu Ala Ala Gln Arg Ala Gln Gln Ala
835 840 845
Gln His Gly Ser Ser Gly Ala Ser Ala Asp Gln Gln Ala Ala Ala Glu
850 855 860
Val Thr Asp Ala Ala Glu Ala Ala Ala Gly Ala Glu Glu Lys Ala Ala
865 870 875 880
Ala Ala Ala Ala Val Arg Ser Gly Lys Pro Pro Ala Ala Glu Tyr Arg
885 890 895
Leu His Ala Val Val Asn His Lys Gly Pro Ala Ala Ser Cys Gly His
900 905 910
Phe Thr Ser Asp Ile Arg Asp Ala Ala Ser Gly Val Trp His Arg Phe
915 920 925
Asp Asp Ser Leu Ala Ser Arg Ile Ala Gly Trp Glu Ala Arg Ser Ser
930 935 940
Glu Lys Gln Arg Glu Cys Tyr Leu Leu Phe Tyr Thr Gly Thr Val Ala
945 950 955 960
Gly Leu Ile Tyr Ser Asp Arg Asp Glu Gly Lys Glu Phe Arg Leu Ala
965 970 975
Asp Phe Arg Thr Val Tyr Thr Phe Glu Ser Trp Ala Ala His Arg Ser
980 985 990
Thr Met Arg Tyr Leu Arg His Phe Met Gly Met Phe Arg Ser Arg Val
995 1000 1005
Leu Cys Gly Leu Arg Val Pro Leu Leu Leu Ile Ala Leu Glu Ala Thr
1010 1015 1020
Leu Val Cys Ser Tyr Glu His Ala Leu Lys Asn Gly Leu Leu Pro Ala
1025 1030 1035 1040
Phe Cys Arg Thr Ile Gln Ile Arg Ala Pro Met Leu Phe Ser Leu Ser
1045 1050 1055
Ser Phe Ala Leu Ser Leu Leu Leu Val Phe Arg Thr Asn Gln Ser Tyr
1060 1065 1070
Ala Arg Phe Asp Glu Gly Arg Arg Leu Trp Ser Val Met Ala Asn Arg
1075 1080 1085
Cys Arg Asp Val Met Arg Gln Ala Cys Ser Tyr Leu Ser Leu Gly Pro
1090 1095 1100
Gln Asn Ala Ala Leu Leu Ala Thr Leu Ser Arg Trp Met Gln Ala Phe
1105 1110 1115 1120
Pro Trp Val Met Lys Asn Tyr Val Arg Glu Ser Lys Ser Trp His Ala
1125 1130 1135
Asp Leu Gly Ser Leu Leu Arg Pro Ala Glu Leu Ala Ala Leu Leu Asp
1140 1145 1150
Thr Arg Asn Pro Pro Leu Leu Thr Leu Gln Val Ile Thr Glu Ile Leu
1155 1160 1165
Asp Arg Met Pro Ile Ser Thr Lys Gln Arg Leu Arg Leu Asp Ile Phe
1170 1175 1180
Met Thr Glu Leu Ser Asp Met Val Gly Ala Ser Glu Arg Leu Ile Thr
1185 1190 1195 1200
Thr Pro Ile Pro Leu Ser Tyr Thr Arg His Thr Ala Arg Phe Leu Ile
1205 1210 1215
Thr Trp Leu Val Leu Thr Pro Phe Thr Ile Phe Ser Ser Cys Gly Trp
1220 1225 1230
Val Thr Ile Phe Ser Ser Val Leu Leu Ala Phe Phe Leu Leu Thr Ile
1235 1240 1245
Glu Glu Ile Gly Val Thr Ile Glu Glu Pro Phe Asn Ile Leu Pro Leu
1250 1255 1260
Glu Ala Ile Asn Thr Arg Ala Ile Asn Thr Arg Val Cys Ala Asp Ile
1265 1270 1275 1280
Ala Arg Tyr Pro Gln Lys Ile Asp Thr Val Val Ser Leu Val Asp Asp
1285 1290 1295
Ala Thr Gly Thr Gln Arg Pro Gln Gln Gln Pro Pro Phe Gln Pro Val
1300 1305 1310
Ala Glu Phe Val Ser Thr Ala Ala Thr Ser Asp Asn Gly Asp Ser
1315 1320 1325
<210> 103
<211> 333
<212> PRT
<213> 小立碗藓
<400> 103
Met His Arg Asn Ser Val Tyr Gly His Gly Glu Trp Lys Lys His Lys
1 5 10 15
Ser Ser Trp Arg His Val Arg His Val Cys Thr Ile Leu Ser Ser Gly
20 25 30
Leu Ile Arg Ala Ile Gly Pro Pro Val Phe Leu Cys Thr Leu Val Ser
35 40 45
Val Phe Val Ala Val Ile Asn His Cys Val Asp Asn Gly Val Leu Pro
50 55 60
Ser Trp Phe Pro Val Leu Lys Val Ala Thr Leu Pro Phe Thr Leu Thr
65 70 75 80
Ser Pro Val Leu Ala Leu Leu Leu Val Phe Arg Thr Asn Thr Ser Tyr
85 90 95
Gln Arg Phe Asp Glu Ala Arg Lys Ala Trp Gly Ser Asn Val Asn Arg
100 105 110
Ala Arg Asp Leu Ala Arg Gln Ala Leu Thr Trp Ile Arg Asn Pro Gly
115 120 125
Asp Ser Lys Lys Leu Gln Cys Leu Leu Arg Tyr Thr Lys Ala Tyr Ser
130 135 140
Phe Cys Leu Met His His Leu Arg Glu Glu Gly Cys Leu Arg Lys Glu
145 150 155 160
Leu Glu Ala Thr Ile Val Asn Glu Glu Glu Val Glu Cys Val Met Asn
165 170 175
Ser Lys Asn Arg Pro Ile Trp Val Leu Gln Val Ile Ser Asp Ile Ile
180 185 190
Asn Glu Cys Gln Ile Thr Pro Trp Glu Arg Ile Ala Met Asp Lys Asn
195 200 205
Ile Thr Gln Phe His Asp Asn Val Gly Ala Cys Glu Arg Ile Phe Lys
210 215 220
Thr Pro Ile Pro Val Ala Tyr Thr Arg Leu Thr Ser Arg Val Leu Thr
225 230 235 240
Leu Trp His Leu Val Leu Pro Phe Ala Leu Trp Glu Thr Cys Gly Trp
245 250 255
His Thr Ile Thr Val Ser Phe Val Ser Ser Ala Ala Leu Phe Tyr Ile
260 265 270
Glu Glu Val Gly Val Met Ile Glu Glu Pro Phe Ser Ile Leu Ala Leu
275 280 285
Ser Thr Ile Cys Thr Gly Ile Val Ala Ala Leu Glu Gly Leu Ser Asn
290 295 300
Ala His Thr Asp Ala Leu Ile Leu Val Trp Gly Ile Val Glu Gln Gln
305 310 315 320
Trp Ser Thr Lys Ala Asp His Leu Met Ile Asn Leu Lys
325 330
<210> 104
<211> 402
<212> PRT
<213> 小立碗藓
<400> 104
Met His Arg Asn Thr Val Tyr Gly His Asp Glu Trp Arg Arg His Lys
1 5 10 15
Ser Ser Trp Arg His Ala Arg His Val Ile Ser Ile Ala Ala Ser Gly
20 25 30
Val Ile Ala Ala Leu Gly Pro Pro Val Leu Leu Ser Thr Ile Thr Ala
35 40 45
Val Phe Val Thr Val Ile Asn His Gly Val Gln His Met Leu Val Ala
50 55 60
Ser Trp Val Pro Tyr Leu Lys Val Ser Pro Ile Pro Phe Thr Phe Ile
65 70 75 80
Ser Pro Val Leu Ala Phe Leu Leu Val Phe Arg Thr Asn Ser Ser Tyr
85 90 95
Gln Arg Phe Asp Glu Ala Arg Lys Val Trp Gly Ser Asn Val Asn Arg
100 105 110
Cys Arg Asp Leu Ala Arg Gln Ala Leu Ser Trp Ile Lys Asn Pro Glu
115 120 125
Asp Ala Ala Arg Leu Glu Cys Leu Leu Arg Phe Leu Lys Ala Tyr Pro
130 135 140
Tyr Tyr Leu Lys Leu His Leu Thr Gln Glu Gly Pro Ser Ser Ser Thr
145 150 155 160
Thr Ser Glu Ile Lys Asp Ile Leu Lys Asp Glu Glu Phe His Lys Val
165 170 175
Ser Leu Val Gln Asn Gln Pro Ile Tyr Val Leu Gln Val Ile Ser Glu
180 185 190
Ile Ile Ser Gln Cys His Ile Pro Asn Trp Glu Lys Ile Cys Met Asp
195 200 205
Ala Asn Leu Thr Gln Phe His Asp Asn Val Gly Ala Cys Glu Arg Ile
210 215 220
Phe Lys Thr Pro Ile Pro Ile Ala Tyr Thr Arg Met Thr Ser Arg Met
225 230 235 240
Leu Ile Met Trp His Leu Ala Leu Pro Tyr Gly Leu Trp Asn Asp Cys
245 250 255
Arg Trp Leu Thr Ile Pro Ala Thr Phe Met Ser Ser Ala Ala Leu Phe
260 265 270
Tyr Ile Glu Gln Val Gly Val Val Ile Glu Glu Pro Phe Cys Ile Leu
275 280 285
Ala Leu Asp Ser Ile Cys Gly Gly Ile Arg Ser Ala Ile Asp Met Phe
290 295 300
Arg Ala Thr Arg Glu Asp Val Phe Leu Leu Thr Glu Ser Ile Arg Ser
305 310 315 320
Glu His Pro Lys Lys Thr Asp Thr Thr Lys Asn Leu Glu Gln Ile Ile
325 330 335
Glu Met Glu Ser Met Asp Ser Asp Pro Leu Glu Glu Thr Ser Lys Glu
340 345 350
Asp Glu Val Met Leu Gly Leu Gly Arg Ser Lys Ser Tyr Lys Ser Tyr
355 360 365
Ser Gly Arg Lys Phe Phe Asp Lys Ser Arg Ser His Ser His Ser Thr
370 375 380
Thr Ser Leu Tyr Gly Asp Leu Asn Val Gly Phe Ser Lys Leu Lys Ser
385 390 395 400
Val Ser
<210> 105
<211> 353
<212> PRT
<213> 小立碗藓
<400> 105
Met His Arg Asn Thr Val Tyr Gly His Thr Glu Trp Arg Arg His Lys
1 5 10 15
Ser Cys Trp Arg His Ser Arg His Leu Ser Ser Ile Phe Ser Ser Gly
20 25 30
Val Ile Phe Thr Leu Gly Pro Pro Val Ile Leu Cys Thr Leu Val Ala
35 40 45
Thr Leu Val Ser Val Ile Asn His Leu Val Gln Asp Arg Arg Leu Pro
50 55 60
Lys Trp Met Pro Leu Leu His Val Ala Ser Leu Pro Phe Thr Leu Thr
65 70 75 80
Ala Pro Val Leu Ala Leu Leu Leu Val Phe Arg Thr Asn Ala Ser Tyr
85 90 95
Ser Arg Phe Asp Glu Ala Arg Lys Ala Trp Gly Ser Asn Val Asn Arg
100 105 110
Thr Arg Asp Leu Ala Arg Gln Ala Leu Thr Trp Ile Arg Met Pro Cys
115 120 125
Asp Ala Pro Lys Leu His Cys Leu Leu Arg His Ile Lys Ala Tyr Ser
130 135 140
Leu Cys Leu Lys Asp His Met Thr Glu Asp Asn Thr Leu Arg Glu Glu
145 150 155 160
Leu Thr Ala Val Leu Glu Pro Ser Glu Val Asp Cys Ala Met Ser Ser
165 170 175
Gln His Arg Pro Asn Tyr Ile Leu Gln Val Met Ser Glu Leu Ile Ser
180 185 190
Gln Cys Gln Ile Ser Gln Trp Glu Lys Ile Thr Met Asp Lys Asn Ile
195 200 205
Thr Ala Phe His Asp Asn Val Gly Ala Cys Glu Arg Ile Phe Lys Thr
210 215 220
Pro Ile Pro Leu Ala Tyr Thr Arg Leu Thr Ser Arg Met Leu Met Phe
225 230 235 240
Trp His Leu Ala Leu Pro Val Gly Leu Trp Asn Thr Cys Gly Trp Leu
245 250 255
Thr Ile Pro Val Ser Phe Met Ser Ala Ala Ala Leu Phe Tyr Ile Glu
260 265 270
Glu Val Gly Val Leu Ile Glu Glu Pro Phe Trp Ile Leu Pro Leu Leu
275 280 285
Ser Ile Ser Gln Gly Ile Cys Ser Ala Val Asp Gly Leu Tyr Val Ala
290 295 300
His Lys Glu Ala Ser Met Leu Trp Ser Ile His Gln Pro Thr Asn Val
305 310 315 320
Asn Asp His Val Ile Ile Ser Phe Asp Gly Ser Arg Ser Ala Gly Ile
325 330 335
Tyr Gln His Asn Asn Ile Asp Val Lys Phe Ser Arg Val Asn Ser Val
340 345 350
Gly
<210> 106
<211> 353
<212> PRT
<213> 小立碗藓
<400> 106
Met His Arg Asn Lys Val Tyr Gly His Ala Glu Trp Ala Lys His Lys
1 5 10 15
Ser Ser Trp Arg His Gly Arg His Leu Met Ser Ile Leu Ser Ser Gly
20 25 30
Val Ile Ser Ala Val Gly Pro Pro Val Leu Ala Cys Thr Leu Leu Ala
35 40 45
Thr Phe Val Thr Val Phe Asn Tyr Phe Val Lys Val Gly Arg Leu Pro
50 55 60
Asn Trp Ile Pro Ile Leu Glu Val Ser Ser Leu Pro Phe Thr Leu Thr
65 70 75 80
Ser Ser Val Leu Ser Leu Leu Leu Val Phe Arg Thr Asn Ser Ser Tyr
85 90 95
Asn Arg Phe Asp Glu Ala Arg Lys Ile Trp Gly Ser Asn Val Asn Arg
100 105 110
Thr Arg Asp Leu Ala Arg Gln Ala Leu Ser Trp Ile Arg Ser Pro Ala
115 120 125
Asp Ala Tyr Lys Leu Ser Cys Leu Leu Arg His Ile Lys Ala Tyr Pro
130 135 140
Phe Ser Leu Lys Asp His Leu Thr Glu Asp Phe Ile Leu Lys Asp Glu
145 150 155 160
Leu Asp Gln Ile Leu Glu Pro Gln Glu Leu Glu Ala Leu Met Ala Thr
165 170 175
Lys His Arg Pro Asn Tyr Ile Leu Gln Val Leu Ser Glu Leu Val Asp
180 185 190
Lys Cys Asn Leu Ser Glu Trp Glu Lys Met Ala Met Asp Glu Asn Ile
195 200 205
Thr Thr Phe His Asp Asn Val Gly Ala Cys Glu Arg Ile Leu Lys Thr
210 215 220
Pro Ile Pro Leu Ala Tyr Thr Leu Val Thr Ser Arg Phe Leu Ile Leu
225 230 235 240
Trp His Leu Val Leu Pro Phe Ala Leu Trp Ala Thr Cys His Trp Leu
245 250 255
Thr Ile Pro Val Thr Phe Leu Thr Ala Thr Ala Leu Phe Tyr Ile Glu
260 265 270
Glu Val Gly Val Leu Ile Glu Glu Pro Phe Trp Ile Leu Pro Leu Leu
275 280 285
Ser Ile Ser Asp Gly Ile Val Ala Ala Leu Asp Gly Leu Ala Ala Ala
290 295 300
His Lys Thr Ser Ser Leu Leu Trp Arg Leu His Gln Pro Asn Lys Asp
305 310 315 320
His Val Val Gln Ile Thr Phe Glu Thr Ser Arg Ser Asp Gly Phe Arg
325 330 335
Lys Ser Arg Asp Val Gly His Val Leu Leu Thr Arg Met Asn Ser Ile
340 345 350
Ala
<210> 107
<211> 391
<212> PRT
<213> 小立碗藓
<400> 107
Met Phe Trp Lys His Pro Ser Gly Arg Thr Ala Ser Ser Glu Gly Phe
1 5 10 15
Val Ile Lys Pro Pro Asp Ser Val Gln Gln Glu Gln Gln Ser Val Asp
20 25 30
Thr Ala Gly Phe Ser Leu Leu Phe His Pro Asn Ala Leu Ser His Lys
35 40 45
His Lys Val Ser Asp Glu Lys Val Ala Trp Gly Glu Thr Ser Lys Gly
50 55 60
Gly Val Ser Gly Gly Gly Gly Gly Gly Ser Val Thr Leu Glu Phe Phe
65 70 75 80
Ser Phe Lys Asn Ala Ser Gln Arg Ser Val Trp Ala Arg Arg Met Gly
85 90 95
Leu His Lys Ser Ser Trp Arg His Gly Arg His Ile Gln Thr Ala Phe
100 105 110
Ser Thr Gly Val Ile Ser Ser Ile Ile Pro Arg Val Phe Phe Cys Thr
115 120 125
Leu Ile Ser Val Leu Val Thr Ile Phe Asn His Ala Val Met Glu Gly
130 135 140
Val Leu Pro His Trp Val Pro Ser Leu Arg Val Pro Thr Leu Pro Met
145 150 155 160
Ser Leu Thr Ala Pro Val Leu Ser Leu Leu Leu Val Phe Arg Thr Asn
165 170 175
Ser Ser Tyr Asn Arg Leu Asp Glu Ala Arg Lys Ala Trp Gly Ser Asn
180 185 190
Val Asn Arg Thr Arg Asp Val Ser Arg Gln Ala Leu Ser Trp Ile Cys
195 200 205
Asp Pro Asp Asp Ala Asp Lys Leu Gln Ser Leu Leu Arg His Ile Lys
210 215 220
Ala Phe Ser Tyr Cys Leu Lys Asp His Leu Thr Gln Glu Asn Leu Leu
225 230 235 240
Gln Glu Glu Leu Ala Arg Val Leu Glu Pro Arg Glu Val Glu Leu Val
245 250 255
Leu Lys Ser Ser His Arg Pro Asn Tyr Val Leu His Val Met Ser Asp
260 265 270
Thr Ile Lys His Cys Arg Ile Ser Lys Trp Glu Ser Lys Ser Met Asp
275 280 285
Arg Asn Ile Thr Gln Phe His Asp Asn Val Gly Ala Cys Glu Arg Leu
290 295 300
Phe Lys Thr Pro Ile Pro Val Ala Tyr Thr Arg Met Ile Ser Arg Phe
305 310 315 320
Leu Ser Ile Trp His Phe Leu Leu Pro Leu Ala Leu Trp Asn Ser Cys
325 330 335
Arg Trp Leu Thr Ile Pro Val Thr Phe Val Ser Gly Val Gly Leu Phe
340 345 350
Cys Ile Glu Glu Val Gly Val Leu Ile Glu Asp Pro Ser Thr Ser Leu
355 360 365
Leu Cys Cys Gln Ser Ala Met Ala Phe Pro Gln Leu Trp Met Ala Ser
370 375 380
Ala Arg Leu Met Lys Glu Leu
385 390
<210> 108
<211> 518
<212> PRT
<213> 产油微藻
<400> 108
Met Pro Gly Ala Leu Ala Thr Ser Leu Asp Glu Pro Asp Arg Pro Gln
1 5 10 15
Arg Ser Val Ser Lys Thr Leu Lys Arg Ala Val Ser Ser Tyr Phe Val
20 25 30
Pro Arg Gly Arg Phe Lys Lys Leu Leu Glu Ser Ala Glu Asn Asp Asn
35 40 45
Glu Ala Asp Val Tyr Arg Arg Asn Ser Gly His Arg Val Phe Thr His
50 55 60
Ala Asp Trp Ala Val His Arg Lys Pro Ser Arg Tyr Ala Arg His Leu
65 70 75 80
Ala Leu Ile Phe Ala Ser Arg Ile Phe Arg Ser Leu Ile Pro Pro Ile
85 90 95
Thr Ala Val Thr Leu Ile Ser Thr Leu Val Gly Val Tyr Glu Thr Leu
100 105 110
Leu Lys Glu His Arg Leu Pro Ala Gln Trp Pro His Val Thr Leu Ala
115 120 125
Leu Gly Gln Gly Phe Gln Leu Thr Ala Phe Ala Leu Ser Leu Leu Leu
130 135 140
Val Phe Arg Thr Asn Ser Ala Tyr Gly Arg Trp Trp Glu Ala Arg Met
145 150 155 160
Leu Trp Gly Ser Leu Thr Asn Arg Thr Arg Asp Phe Val Arg Gln Cys
165 170 175
Leu Ala Phe Phe Pro Glu Asp Ala Lys Arg Leu Arg Glu Ala Ala Val
180 185 190
Leu Trp Thr Ile Ala Leu Pro Tyr Val Val Lys Ala His Leu Arg Glu
195 200 205
Gly Asn Arg Leu Glu Ser Glu Leu Glu Gly Ile Leu Thr Arg Asp Glu
210 215 220
Val Asp Ala Leu Thr Gly Ala Thr His Arg Pro Cys Phe Val Leu Ala
225 230 235 240
Ala Leu Ser Gln Val Val Ala Ala Ala Gly Leu Asp Pro Gln Leu Arg
245 250 255
Gln Arg Leu Asp Thr Asn Leu Thr Ala Phe Glu Asp Cys Ile Gly Gly
260 265 270
Cys Glu Arg Ile Leu Arg Thr Pro Ile Pro Gln Ser Tyr Thr Arg His
275 280 285
Thr Ser Arg Phe Leu Met Ile Trp Leu Ile Met Met Pro Phe Thr Leu
290 295 300
Trp Ala Ala Tyr Ser Trp Val Ser Ile Leu Leu Ser Gly Leu Phe Ala
305 310 315 320
Tyr Leu Met Leu Gly Ile Asp Glu Ile Gly Val Gln Ile Glu Glu Pro
325 330 335
Phe Gly Val Leu Pro Leu Gly Ser Phe Cys Ala Thr Ile Glu Arg Asn
340 345 350
Leu Arg Glu Leu His Tyr Ser Asn Pro Glu Ala Arg Phe Ala Ala Leu
355 360 365
Pro Trp Leu Val Pro Ala Ser Pro Thr Cys Ser Arg Ala Ser Gly Ser
370 375 380
Cys Asp Gly Ala Asp Met Ala Ala Gly Met Asp Asp Ile Asp Pro Val
385 390 395 400
Ala Gly Lys Leu Pro His Leu Asn Gly Ala Pro Gln Ala Gly Gln Pro
405 410 415
Arg Val Tyr Lys His Thr Ser Asp Ala Ala Glu Leu Ala Ala Gly His
420 425 430
Val Arg Phe Ala His Arg Trp Leu Glu Pro His Pro His Leu Ile His
435 440 445
Ser Pro Val His Pro His His Pro Asn His His His Gly His Gly His
450 455 460
Gly His Gly Ala Gln Glu His Gly Arg Gly Ala Phe His Gln Thr Ser
465 470 475 480
Thr Leu His Ala Asp Val Glu Asp Gln Val Val His Pro Ala Ser Tyr
485 490 495
Pro Thr Ala Ala Arg His Thr Pro Pro His Arg Ser Trp Phe Gly Gly
500 505 510
Arg Trp Val Glu Glu Asp
515
<210> 109
<211> 412
<212> PRT
<213> 风铃辣椒
<400> 109
Met Thr Asn Pro Arg Ser Leu Phe Asn Ile Gln Ser Pro Thr Asn Ala
1 5 10 15
Ser Leu Ser Ser His Phe Thr Leu Lys Lys Pro Ser Lys Leu Leu Ser
20 25 30
Tyr Lys Lys Leu Thr Phe Cys Ser Phe Lys Val Leu Cys Ser Ser Gln
35 40 45
Pro Ser Lys Ala Pro Gln Asn Ser Asn Leu Thr Ser Thr Leu Val Ser
50 55 60
Ile Leu Arg Val Ile Pro Asp Trp Ala Asp Lys Ile Gln Glu Gly Gly
65 70 75 80
Met Arg Lys Lys Arg Ser Leu Tyr Lys His Glu Thr Trp Val Gln His
85 90 95
Arg Ser Ser Leu Arg His Leu Arg His Leu Phe Ser Ser Phe Asn Ser
100 105 110
Arg Val Val Leu Ser Leu Ile Pro Pro Val Ile Ala Phe Thr Ser Phe
115 120 125
Ala Phe Val Ile Ala Ser Tyr Asn Ser Ala Val Ser Leu His Trp Leu
130 135 140
Pro Glu Phe Phe Pro Leu Leu Arg Ala Ser Pro Gln Pro Tyr Gln Leu
145 150 155 160
Thr Ala Pro Ala Leu Ala Leu Leu Leu Val Phe Arg Thr Glu Ala Ser
165 170 175
Tyr Ala Arg Phe Glu Thr Gly Lys Lys Ala Trp Thr Asn Val Ile Ala
180 185 190
Gly Thr Asn Asp Phe Ala Arg Gln Val Ile Thr Ala Cys Val Asp Lys
195 200 205
Arg Asp Gly Val Leu Lys Glu Ala Leu Leu Gln Tyr Ile Met Ala Phe
210 215 220
Pro Val Ala Leu Lys Cys His Ile Val Tyr Gly Ser Asp Leu Ala Ser
225 230 235 240
Asp Leu Lys Asn Leu Leu Glu Ala Asp Asp Leu Ala Val Val Leu Ser
245 250 255
Ser Lys His Arg Pro Arg Cys Ile Ile Gly Phe Ile Ser Gln Ser Leu
260 265 270
Gln Ser Leu Asn Leu Glu Gly Thr Lys Leu Ser Gln Leu Glu Ser Lys
275 280 285
Ile Ser Cys Phe His Glu Gly Ile Gly Val Cys Glu Gln Leu Ile Gly
290 295 300
Ile Pro Ile Pro Leu Ser Tyr Thr Arg Leu Thr Ser Arg Phe Leu Val
305 310 315 320
Leu Trp His Leu Thr Leu Pro Ile Ile Leu Trp Asp Asp Cys His Trp
325 330 335
Leu Val Val Pro Ala Thr Phe Ile Ser Ala Ala Ser Leu Phe Cys Ile
340 345 350
Glu Glu Val Gly Val Leu Ile Glu Glu Pro Phe Pro Met Leu Ala Leu
355 360 365
Asp Glu Leu Cys Gln Arg Val His Asp Asn Ile Gln Glu Ala Met Ala
370 375 380
Ser Glu Lys Gln Ile Gln Glu Arg Leu Asn Thr Lys Arg Lys Arg His
385 390 395 400
Phe Ser Glu Asn Ser Pro Asn Gly Trp Pro Thr Ser
405 410
<210> 110
<211> 435
<212> PRT
<213> 胶球藻 C-169
<400> 110
Met Ile Ala Thr Ile His Thr Leu Tyr Tyr Gln Ala Gly Leu Gln Lys
1 5 10 15
Gly Phe Gln Thr Pro Arg Pro Arg Trp Arg Lys Leu His Tyr Ser Arg
20 25 30
Ala Pro Gly Lys Leu Ser Arg Asn Leu Leu Val Arg Cys Gln Glu Thr
35 40 45
Asp Ile Glu Trp Val Glu Arg Ala Ala Ile Gln Arg Glu Ala Trp Lys
50 55 60
Glu Glu Ser Arg Lys Tyr Arg Arg Val Thr Phe Thr Tyr Asp Asp Trp
65 70 75 80
Val Ala His Arg Ser Thr Thr Arg Tyr Ser Arg His Leu Ala Gly Ile
85 90 95
Leu Asp Ser Arg Ile Phe Arg Gly Leu Leu Pro Thr Leu Thr Ala Val
100 105 110
Met Met Val Ala Thr Phe Val Ser Val Tyr Glu Thr Leu Arg Glu Ala
115 120 125
Gly Ile Leu Ile Pro His Asp Trp Val Gly Leu Thr Val Glu Ala Gly
130 135 140
Gln Ala Phe Asn Leu Thr Ser Phe Ala Leu Ser Leu Leu Leu Val Phe
145 150 155 160
Arg Thr Asn Glu Ser Tyr Ser Arg Trp Leu Glu Ala Arg Lys Ala Trp
165 170 175
Thr Asn Ile Val Thr Arg Ser Arg Asp Phe Ala Arg Gln Gly Leu Ser
180 185 190
Trp Leu Ser Gly Asp Lys Gln Arg Glu Ser Met Leu Glu Arg Trp Thr
195 200 205
Ile Ala Phe Ile Lys Cys Thr Met Ala His Val Arg Glu Glu Cys Asp
210 215 220
Leu Gln Ser Leu Leu Glu Asp Val Leu Glu Ala His Glu Leu Glu Gln
225 230 235 240
Leu Met Ala Ala Gln His Lys Pro Asn Phe Val Leu His Val Leu Ser
245 250 255
Glu Leu Val Trp Gly Ala Asn Leu Met Glu Gly Gln Ala Met Arg Met
260 265 270
Asp Glu Ala Leu Thr Val Phe Gly Glu Gln Val Gly Thr Cys Glu Arg
275 280 285
Leu Leu Lys Thr Pro Ile Pro Leu Ser Tyr Thr Arg His Thr Ser Arg
290 295 300
Phe Leu Val Met Trp Leu Ala Phe Leu Pro Phe Ser Leu Trp Asp Ala
305 310 315 320
Cys His Trp Val Thr Ile Pro Ala Ser Gly Ile Ile Ala Phe Leu Leu
325 330 335
Leu Gly Ile Glu Glu Ile Gly Val Gln Ile Glu Glu Pro Phe Gly Ile
340 345 350
Leu Pro Leu Gly Ala Trp Ile Leu Phe His Thr Ile Glu Ala Asp Ser
355 360 365
Val Ile Asp Pro Gln Ser Phe Asp Cys Thr Ile Gln Gln Ser Val Lys
370 375 380
Gly Ala Leu Ser Leu Ala Leu Leu Leu Arg Met Thr Gly Cys Ala Arg
385 390 395 400
Arg Ala Ile Gln Arg Cys Cys Cys Gly Arg Asp Gln Thr Asp Gly Ala
405 410 415
Arg His Glu Cys Ala Ser Ser Gly Gly Gly Arg Pro Glu Arg Glu Arg
420 425 430
Cys Lys Arg
435
<210> 111
<211> 474
<212> PRT
<213> 胶球藻 C-169
<400> 111
Met Ser Arg Leu Asn His His Ala Leu Phe Glu Asp His Val Val Thr
1 5 10 15
Ala Ala Asp Trp Tyr His Leu Arg Ser Trp Arg Arg His Leu Pro Asp
20 25 30
Leu Thr Trp Val Thr Val Leu Ser Gly Phe Trp Leu Trp Val Val Tyr
35 40 45
Phe Ala Leu Ile Ala Val Leu Ile Gly Leu Tyr His Thr Val Leu Val
50 55 60
Pro Arg Gly Ala Pro Asp Trp Pro Pro Lys Asn Val Ala Ser Val Leu
65 70 75 80
Tyr Gln Pro Phe Ala Ile Thr Ser Phe Ala Leu Ala Leu Leu Met Val
85 90 95
Phe Arg Thr Asn Ser Ser Tyr Ala Arg Trp Trp Glu Ala Arg Thr Val
100 105 110
Trp Gly Gln Val Phe Asn Val Thr Arg Asn Leu Val Arg Gln Ala Asp
115 120 125
Ala Trp Phe Gly Glu Glu Asp Val Ala Ala Phe Gln Met Leu Val Arg
130 135 140
Trp Cys Ala Ala Ala Gly Tyr Ile Leu Gln Ala His Leu Cys Thr Ala
145 150 155 160
Lys Leu Gly Pro Glu Ala Glu Gly Leu Leu His Pro Glu Glu Leu Lys
165 170 175
Leu Leu Ala Ser Trp Glu His Arg Pro Ile Cys Ala Gly Gln Val Leu
180 185 190
Ser Ser Ile Val Ala Ser Ala Val Lys Asp Ser Gln Leu Arg Ala Ala
195 200 205
Met Asp Asp Gln Ile Ala Thr Tyr Ile Asn Asp Ala Gly Ala Cys Glu
210 215 220
Arg Ile Gln Arg Thr Cys Ile Pro Phe Cys Tyr Thr Arg His Thr Ser
225 230 235 240
Arg Phe Leu Ile Leu Trp Leu Thr Phe Leu Pro Phe Ala Leu Trp Glu
245 250 255
Ile Cys Gly Trp Ala Ser Pro Val Ala Glu Ala Val Leu Ala Phe Leu
260 265 270
Leu Met Gly Val Glu Asn Ile Gly Ile Gln Ile Glu Glu Pro Phe His
275 280 285
Val Leu Pro Met His Ser Tyr Cys Ala Val Ile Ala Lys Asn Ala Leu
290 295 300
Glu Val Ala Arg Glu Arg Lys Gly Leu Asp Leu Phe Val Glu Glu Ala
305 310 315 320
Leu Arg Arg Glu Leu Cys Met Glu Pro Leu Gly Lys Leu Glu Asn Gly
325 330 335
Gly Ala Gly Ala Asp Ser Asn Val Phe Val Arg Pro Ser Gly Ala Ala
340 345 350
Gly Val Ser His Lys Ala Ser Pro Arg Pro Thr Asn Pro Ser Leu Arg
355 360 365
Val Arg Cys Gln Ser His Arg His Asn Ala Asp Pro Ser Leu His Glu
370 375 380
Ala Arg Gly Val Phe Gln Met Val Pro Gln Arg Gly Glu Ala Thr Leu
385 390 395 400
Leu Asp Ala Arg Asp Ala Leu Glu Thr Cys Ser Ala Leu Asn Asp Ala
405 410 415
Ser Ser Lys Glu Ala Cys Tyr Ala Thr Phe Gly Cys Asp Gly Arg Arg
420 425 430
Val Glu Lys Tyr Phe Gly Ala Val Glu Val Leu Glu Thr Ala Trp Glu
435 440 445
Gln Val Lys Glu Glu Glu Val Glu Asp Asp Asp Glu Phe Val Glu Glu
450 455 460
Asn Leu Ser Pro Trp Pro Trp Asp Gly Arg
465 470
<210> 112
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 112
gacaccaaga ccatcctggc 20
<210> 113
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 113
aacagaactg cagaggtccc g 21
<210> 114
<211> 17
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 114
cggtgcccat gagctcc 17
<210> 115
<211> 17
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 115
gccactaacc ggcccaa 17
<210> 116
<211> 18
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 116
aatcccgtcc atgtcgct 18
<210> 117
<211> 18
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 117
cggcttgtga ggacctcg 18
<210> 118
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 118
gccagaagga ctcgtacgtt 20
<210> 119
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 119
cgccagagtc cagcacgata 20
<210> 120
<211> 48
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 120
gctactcaca acaagcccag ttatgcagat gcaagcaaac cgttcgtc 48
<210> 121
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 121
gagccaccca gatctccgtt cttgcgctcc ccacccatgg 40
<210> 122
<211> 40
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 122
gctactcaca acaagcccag ttatggccac tggtcagacc 40
<210> 123
<211> 37
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 123
gagccaccca gatctccgtt tctccttgtc tccgcac 37
<210> 124
<211> 46
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 124
gctactcaca acaagcccag ttatgcaagt cagcaaggtt ccctcg 46
<210> 125
<211> 38
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 125
gagccaccca gatctccgtt ccggggcgag atgcgcac 38
<210> 126
<211> 90
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 126
ctagtgggag cgagttgcaa ggcatatctc gctgatcggc accatggggg tggtggtgat 60
cagcgctata tgttttgcaa ctcgctcccg 90
<210> 127
<211> 90
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 127
ctagcgggag cgagttgcaa aacatatagc gctgatcacc accaccccca tggtgccgat 60
cagcgagata tgccttgcaa ctcgctccca 90
<210> 128
<211> 90
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 128
ctagtgagag cgtgttgcaa ggcatatctc gctgatcggc accatggggg tggtggtgat 60
cagcgctata tgttttgcaa cacgctctcg 90
<210> 129
<211> 90
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 129
ctagcgagag cgtgttgcaa aacatatagc gctgatcacc accaccccca tggtgccgat 60
cagcgagata tgccttgcaa cacgctctca 90
<210> 130
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 130
gctgtgtggc attgaggaga 20
<210> 131
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 131
ggatgaggct gatgagtccg 20
<210> 132
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 132
acggtctacg acttccctca 20
<210> 133
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 133
ttggatcacg tgggattggg 20
<210> 134
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 134
aagtcagcaa ggttccctcg 20
<210> 135
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 135
tgaatgagcc tagcgggttg 20
<210> 136
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 136
atgtgctgtc cgtggctttc 20
<210> 137
<211> 22
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 137
cagaccttga ccatcttgtc cc 22
<210> 138
<211> 18
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 138
tgccccttct cagcacgt 18
<210> 139
<211> 18
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 139
actgcctcac actcccct 18
<210> 140
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 140
gcaccaatca tgtcaagcct 20
<210> 141
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 141
gacgttacag cacacccttg 20