阅读说明：本技术 一种清痘修护面膜及其制备方法 (Acne-clearing repairing mask and preparation method thereof ) 是由 陈勇 邱威 罗兴义 冯晓月 王春晓 于 2021-10-19 设计创作，主要内容包括：本发明提供了一种清痘修护面膜,包括水、皮肤调理剂、保湿剂、增稠剂和pH调节剂,所述皮肤调理剂包括表达十三肽的酵母菌发酵产物滤液、水解原花青素、神经酰胺、二(月桂酰胺谷氨酰胺)赖氨酸钠、甘草酸二钾中的一种或几种。研究结果表明本发明所述面膜具有保湿、控油、祛痘印、改善炎症性丘疹、去红、改善皮肤角质层细胞剥落和皮损中的功效。的作用,可以应用于皮肤美容、皮肤护理等皮肤修复产品。(The invention provides an acne-clearing repairing mask which comprises water, a skin conditioner, a humectant, a thickening agent and a pH regulator, wherein the skin conditioner comprises one or more of yeast fermentation product filtrate for expressing tridecapeptide, hydrolyzed procyanidine, ceramide, sodium bis (lauramide glutamine) lysine and dipotassium glycyrrhizinate. Research results show that the mask disclosed by the invention has the effects of moisturizing, controlling oil, removing acne marks, improving inflammatory pimples and red skin, and improving the peeling of skin stratum corneum cells and skin lesions. Can be applied to skin repair products for skin beauty, skin care and the like.)

1. An acne-clearing repairing mask comprises water, a skin conditioner, a humectant, a thickening agent and a pH regulator, and is characterized in that the skin conditioner comprises one or more of yeast fermentation product filtrate for expressing tridecapeptide, hydrolyzed procyanidine, ceramide, sodium bis (lauramide glutamine) lysine and dipotassium glycyrrhizinate.

2. The acne removal facial mask according to claim 1, wherein the yeast fermentation product filtrate expressing tridecapeptide is selected from one or more of a galactose yeast fermentation product filtrate and a Pichia pastoris fermentation lysate filtrate.

3. The acne-removing face mask according to claim 1, wherein the ceramide is selected from one or more of ceramide 2, ceramide 3 and ceramide 6 II.

4. The acne-removing face mask according to claim 1, wherein the humectant is one or more selected from glycerin, polyethylene glycol 8, sodium hyaluronate and 1, 2-hexanediol; the thickening agent is selected from one or more of hydroxyethyl cellulose, xanthan gum and carbomer; the pH regulator is arginine.

5. The acne-removing facial mask according to claim 1, wherein the skin conditioner further comprises one or more of glycerin, caprylic/capric triglyceride, octyldodecanol and hydrogenated lecithin.

6. The acne-removing face mask according to any one of claims 1-5, which is characterized by comprising the following components in parts by weight:

70.0 to 90.0 portions of water, 0.1 to 30.0 portions of skin conditioner, 0.1 to 20 portions of humectant, 0.01 to 2.0 portions of thickening agent and 0.01 to 2.0 portions of pH regulator.

7. The acne-removing repairing mask as claimed in claim 6, which is characterized by comprising the following components in parts by weight: 70.0-90.0 parts of water, 8-30.0 parts of skin conditioner, 1.0-20 parts of humectant, 0.2-2.0 parts of thickening agent and 0.3-2.0 parts of pH regulator.

8. A method for preparing the acne-removing repairing mask as claimed in any one of claims 1-7, wherein the water, humectant and thickener are added into a water phase pot, stirred uniformly and heated to 80-85 ℃, pumped into an emulsifying pot, stirred and insulated for 0.1-1 hour, cooled to 50 ℃, added with skin conditioner and pH regulator, stirred uniformly, and cooled to 35 ℃ continuously and discharged.

9. Use of a mask according to any one of claims 1 to 7 in the manufacture of a skin cosmetic or skin care product.

10. The use of claim 9, wherein said product has the effects of moisturizing, controlling oil, removing acne marks, ameliorating inflammatory papules, removing red blood, ameliorating skin cuticle cell flaking and skin lesions.

Technical Field

The invention belongs to the technical field of daily cosmetics, and particularly relates to an acne-removing repairing mask and a preparation method thereof.

Background

Acne is commonly called as whelk, is a chronic inflammatory dermatosis which involves hair follicle sebaceous glands and is mainly clinically manifested by comedo, papule, nodule, pustule, cyst and the like. It is commonly found in the abundant sebaceous glands of the head, face, neck, chest and back. The causes of acne are mainly: (1) endocrine factors: after puberty, levels of androgens, particularly testosterone, rise rapidly in the body, which also promotes the sebaceous glands to become active and produce a large amount of oil. (2) Plugging of pilosebaceous ducts: the duct of the pilosebaceous gland is hyperkeratotic, the aperture of the duct becomes small, narrow or blocked, and cells and sebum shed from the hair follicle wall can not be normally discharged to form the white head acne which is commonly called closed acne. (3) Microbial infection: under the accumulation of cells shed from the hair follicle wall and sebum, propionibacterium acnes, the first bacterium, multiplies, leading to an inflammatory response (4) inflammatory factors: propionibacterium acnes can stimulate keratinocytes to up-regulate the expression of TLR2 gene, activate NF-kB signal pathway and increase the expression of inflammatory factors IL-6, IL-8 and TNF-alpha in cells; in addition, Propionibacterium acnes can produce polypeptides that chemotact neutrophils, activate complement, and cause leukocytes to release various enzymes, exacerbating inflammation. (5) Genetic factors.

In addition, acne can be induced or exacerbated by damage to the stratum corneum in the epidermis of the skin caused by dietary irritation, irregular work and rest, environmental pollution, sun exposure, stress, obesity, and improper cosmetic use. For example, the skin has low water content due to strong ultraviolet rays, dry and cold climate, allergy, damaged acid-base state of the skin and other factors in daily life, so that cells are damaged, the normal metabolism of the cells is influenced, the imbalance of water and oil balance is caused, and the deposition of pigments is accelerated; dust and suspended matters in the air block skin pores, so that normal cell metabolism is influenced; modern people have high living pressure, fast living rhythm, insufficient sleep, frequent staying up all night, no love for drinking water, frequent eating of spicy and irritating food, low fruit intake and other bad living habits influence the metabolism of cells to be in a tense state for a long time, all cause skin problems and induce acne.

The facial mask can keep sufficient nutrition and moisture of facial skin, so that nutrient substances in the facial mask can effectively permeate into the skin, the metabolism of epithelial tissue cells is promoted, the formation of papules, pustules and cysts is reduced or even avoided, and the facial skin is clean and moist.

Disclosure of Invention

The invention provides an acne-removing and repairing facial mask which can be applied to skin beautifying and skin nursing, is particularly suitable for the conditions of dry skin and the like in the beautifying and nursing, and has the functions of moisturizing, antioxidation and anti-inflammation.

The specific technical scheme of the invention is as follows:

an acne-removing repairing mask comprises water, a skin conditioner, a humectant, a thickening agent and a pH regulator, wherein the skin conditioner comprises one or more of yeast fermentation products expressing tridecapeptide, hydrolyzed procyanidine, ceramide, sodium dilauramide glutamine lysine and dipotassium glycyrrhizinate.

The tridecapeptide is a bioactive polypeptide obtained by microbial fermentation, the amino acid sequence of the tridecapeptide is CysHeArgValCysTyrArgGlyIleCys (SEQ ID No: 1), the tridecapeptide has the main effects of resisting bacteria and diminishing inflammation, promoting wound healing, repairing skin, reducing scar formation, improving skin and mucous membrane immunity and inhibiting skin bacterial growth, and can be used for treating acne, dermatitis and skin injury. Can be purchased commercially or prepared by the method disclosed in Chinese patent document CN108852900A, namely a tridecapeptide-containing antibacterial facial mask and a preparation method thereof.

The yeast fermentation product filtrate for expressing the tridecapeptide can be selected from one or more of galactose yeast-like bacteria fermentation product filtrate and pichia pastoris fermentation lysate filtrate.

The ceramide is preferably one or more of ceramide 2, ceramide 3 and ceramide 6 II.

The humectant is selected from one or more of glycerol, polyethylene glycol 8, sodium hyaluronate and 1, 2-hexanediol; the thickening agent is selected from one or more of hydroxyethyl cellulose, xanthan gum and carbomer; the pH regulator is arginine.

Preferably, the skin conditioning agent further comprises one or more of glycerin, caprylic/capric triglyceride, octyldodecanol, and hydrogenated lecithin.

In a specific example of the invention, the acne-removing protective mask comprises water, yeast fermentation product filtrate expressing tridecapeptide, hydrolyzed procyanidin, ceramide 2, ceramide 3, ceramide 6II, sodium di (lauramide glutamine) lysine, dipotassium glycyrrhizinate, glycerol, polyethylene glycol 8, sodium hyaluronate, 1, 2-hexanediol, caprylic/capric triglyceride, octyldodecanol, hydrogenated lecithin, hydroxyethyl cellulose, xanthan gum, carbomer and arginine.

Preferably, the acne-removing and repairing mask disclosed by the invention comprises the following components in parts by weight: 70.0 to 90.0 portions of water, 0.1 to 30.0 portions of skin conditioner, 0.1 to 20 portions of humectant, 0.01 to 2.0 portions of thickening agent and 0.01 to 2.0 portions of pH regulator. Preferably, the acne-removing and repairing mask comprises the following components in parts by weight: 70.0-90.0 parts of water, 8-30.0 parts of skin conditioner, 1.0-20 parts of humectant, 0.2-2.0 parts of thickening agent and 0.3-2.0 parts of pH regulator.

The acne-removing repairing mask disclosed by the invention can be prepared by adopting the following method:

adding water, humectant and thickener into water phase pot, stirring, heating to 80-85 deg.C, pumping into emulsifying pot, stirring, maintaining the temperature for 0.1-1 hr, cooling to 50 deg.C, adding skin conditioner and pH regulator, stirring, cooling to 35 deg.C, and discharging.

And (3) soaking a membrane cloth into the prepared mask liquid by adopting the prior art to obtain the application type mask.

The invention also aims to provide application of the mask in preparing skin care products for skin beautifying, skin care and acne removal.

The invention has the advantages that:

ceramide is a sphingolipid composed of sphingosine long-chain base and fatty acid. Ceramide (Ceramide) is a kind of phospholipid with Ceramide as a skeleton, mainly comprising Ceramide phosphorylcholine and Ceramide phosphorylethanolamine, the phospholipid is a main component of a cell membrane, 40-50% of sebum in a horny layer is composed of Ceramide, and the Ceramide is a main part of an intercellular matrix and plays an important role in keeping the moisture balance of the horny layer. The skin-activating essence cosmetic containing ceramide can enhance the anti-aging function of skin, keep the skin elastic, smooth and delicate, and reduce the formation of facial wrinkles.

The tridecapeptide is bioactive polypeptide obtained by microbial fermentation, has the main effects of resisting bacteria and diminishing inflammation, promoting wound healing, repairing skin, reducing scar formation, improving skin and mucous membrane immunity, and inhibiting skin bacterial growth, and can be used for treating acne, dermatitis and skin injury. Therefore, the product can be used in medicine and cosmetics, and is a good raw material for functional cosmetics.

The detection result shows that the mask disclosed by the invention can obviously improve the skin percutaneous water loss, has the effects of controlling oil and fading acne marks, and obviously improves the skin problems of inflammatory pimples, skin redness, cuticle cell peeling, skin damage and the like by using the mask disclosed by the invention for 28 consecutive days by 33 healthy male and female subjects in China. The subjective evaluation results showed that: the whole using feeling is better, and after 28 days of use, the comprehensive evaluation of the testee is satisfied with the mask of the invention.

Drawings

Fig. 1 shows the skin moisture loss rate through skin after using the mask of the present invention, indicating the test results ("+" indicates significant difference, "+" 0.001 ≦ P <0.01, "+" P < 0.001).

Fig. 2 shows the results of the skin oil content test using the mask of the present invention ("+" indicates significant difference, P is 0.01. ltoreq. P < 0.05).

Figure 3 is the skin melanin value (pox mark) after using the mask of the present invention ("+" indicates significant differences, "+" P < 0.001).

Figure 4 is the results of a skin erythrosin value (inflammatory papules) test using the mask of the present invention ("x" indicates a significant difference, "x" P < 0.001).

Fig. 5 shows the results of the red area ratio test using the mask of the present invention ("+" indicates significant difference, "+" P < 0.001).

FIG. 6 shows an example of red area improvement (Cross polarized light pattern) in a subject using the mask of the present invention.

Figure 7 shows the results of the stratum corneum cell exfoliation test using the mask of the present invention ("+" indicates significant differences, "+" P < 0.001).

Fig. 8 shows the results of the evaluation of FENC by the dermatologist after using the mask of the present invention ("+" indicates significant differences, "+" P < 0.001).

Fig. 9 shows an example of skin damage improvement (Standard 2 illuminant mode) of a subject using the mask of the present invention.

Detailed Description

The following examples illustrate specific steps of the present invention, but are not intended to limit the invention.

Terms used in the present invention generally have meanings commonly understood by those of ordinary skill in the art, unless otherwise specified. The present invention is described in further detail below with reference to specific examples and with reference to the data. It will be understood that these examples are intended to illustrate the invention and are not intended to limit the scope of the invention in any way.

In the following examples, various procedures and methods not described in detail are conventional methods well known in the art.

Example 1 preparation of mask of the invention

Table 1 formulation 1

According to the formula 1, the water, the humectant and the thickening agent in corresponding parts by weight are weighed and added into a water phase pot, the mixture is heated to 80-85 ℃ after being uniformly stirred, and the mixture is pumped into an emulsifying pot, stirred and kept warm for 0.1-1 hour. Cooling to 50 deg.C, adding skin conditioner and pH regulator, stirring, cooling to 35 deg.C, and discharging.

Example 2 preparation of mask of the present invention

TABLE 2 formulation 2

According to the formula 2, the water, the humectant and the thickening agent in corresponding parts by weight are weighed and added into a water phase pot, the mixture is heated to 80-85 ℃ after being uniformly stirred, and the mixture is pumped into an emulsifying pot, stirred and kept warm for 0.1-1 hour. Cooling to 50 deg.C, adding skin conditioner and pH regulator, stirring, cooling to 35 deg.C, and discharging.

Example 3 preparation of mask of the present invention

Table 3 formulation 3

According to the formula 3, the water, the humectant and the thickening agent in corresponding parts by weight are weighed and added into a water phase pot, the mixture is heated to 80-85 ℃ after being uniformly stirred, and the mixture is pumped into an emulsifying pot, stirred and kept warm for 0.1-1 hour. Cooling to 50 deg.C, adding skin conditioner and pH regulator, stirring, cooling to 35 deg.C, and discharging.

Example 4 preparation of mask of the present invention

Table 4 formulation 4

According to the formula 4, the water, the humectant and the thickening agent in corresponding parts by weight are weighed and added into a water phase pot, the mixture is heated to 80-85 ℃ after being uniformly stirred, and the mixture is pumped into an emulsifying pot, stirred and kept warm for 0.1-1 hour. Cooling to 50 deg.C, adding skin conditioner and pH regulator, stirring, cooling to 35 deg.C, and discharging.

Example 5 preparation of mask of the present invention

TABLE 5 formulation 5

According to the formula 5, the water, the humectant and the thickening agent in corresponding parts by weight are weighed and added into a water phase pot, the mixture is heated to 80-85 ℃ after being uniformly stirred, and the mixture is pumped into an emulsifying pot, stirred and kept warm for 0.1-1 hour. Cooling to 50 deg.C, adding skin conditioner and pH regulator, stirring, cooling to 35 deg.C, and discharging.

Example 6 preparation of mask of the present invention

Table 6 formulation 6

According to the formula 6, the water, the humectant and the thickening agent in corresponding parts by weight are weighed and added into a water phase pot, the mixture is heated to 80-85 ℃ after being uniformly stirred, and the mixture is pumped into an emulsifying pot, stirred and kept warm for 0.1-1 hour. Cooling to 50 deg.C, adding skin conditioner and pH regulator, stirring, cooling to 35 deg.C, and discharging.

Example 7 evaluation of the efficacy of the mask of the present invention

1. The test method comprises the following steps: the method refers to a method for testing the oil control efficacy of the T/ZHCA 002-2018 cosmetic.

2. Subject: 33 healthy males and females ranged in age from 19-45 years with a mean age of 31.09 years. Inclusion criteria were: oily skin (forehead oil content is greater than or equal to 90 μ g/cm2, at least 1 inflammatory papule, 1 acne, and at least one black pox mark on face).

3. Mask sample: the masks of examples 1-6 of the present invention. 4. The using method comprises the following steps: after a subject cleans the face and refreshes the skin, a proper amount of the facial mask is applied on the face, stays for 10 to 15 minutes, is cleaned by clean water, is used once every two days, and is applied for 15 to 20 minutes each time. The use period is 28 days.

4. Test flow

D0：

1) Registering the information of the testee, screening the face by a laboratory technician (the screening fraction of lactic acid is more than or equal to 3 points, the fat content of the forehead is more than or equal to 90 mu g/cm2), and signing an informed consent after the selection and elimination standards are finished;

2) the subject cleans the face with the face cleaning product, and wipes the skin dry with dry facial tissue, sits still for 30min in a laboratory at a temperature of 21 + -1 deg.C and a humidity of 50 + -10% RH;

3) test subject skin base value: testing the skin percutaneous water loss rate, the oil content, the hemachrome content of the pox part and the melanin content of the pox mark part of a test subject at the cheek part by a laboratory technician, photographing by Visia-CR, testing the skin cuticle cell exfoliation by VC20, and evaluating the quantity of facial acnes and pimples by an expert;

4) the laboratory technician instructing the subject to use the product according to the product use requirements and providing written test precautions and instructions for use;

5) the subjects received the product and used a diary and left the laboratory.

D7：

1) The subject was asked by phone to use the product for 7 days with or without facial discomfort.

D14：

1) The test subject visits again, cleans the face with the face cleaning product, and wipes the skin with dry facial tissue, sits still for 30min in a laboratory with the temperature of 21 + -1 deg.C and the humidity of 50 + -10% RH;

2) test subject D14 skin data values: testing the skin percutaneous water loss rate, the oil content, the hemachrome content of the pox part and the melanin content of the pox mark part of a test subject at the cheek part by a laboratory technician, photographing by Visia-CR, testing the skin cuticle cell exfoliation by VC20, and evaluating the quantity of facial acnes and pimples by an expert;

3) the subject filled out a questionnaire at D14;

4) the subject signs off.

D21：

1) The phone asks the subject for 21 days of use of the product, with or without facial discomfort.

D28：

1) The test subject visits again, cleans the face with the face cleaning product, and wipes the skin with dry facial tissue, sits still for 30min in a laboratory with the temperature of 21 + -1 deg.C and the humidity of 50 + -10% RH;

2) test subject D28 skin data values: testing the skin percutaneous water loss rate, the oil content, the hemachrome content of the pox part and the melanin content of the pox mark part of a test subject at the cheek part by a laboratory technician, photographing by Visia-CR, testing the cheek cuticle peeling parameter by VC20, and evaluating the quantity of facial acnes and pimples by an expert;

3) the subject filled out a questionnaire at D28;

4) subjects returned the product and used a diary.

4. The test items are shown in table 7.

TABLE 7

5. Test results

The results show that the facial masks prepared in examples 1-6 can effectively improve the water loss of skin through skin in the above test items, have the effects of controlling oil and lightening acne marks, and remarkably improve the skin problems of inflammatory pimples, skin redness, cuticle cell peeling, skin damage and the like. For the sake of economy, the test results of the mask prepared in example 5 will not be described. 1) The skin transdermal water loss rate refers to the test results as shown in table 8 and fig. 1.

TABLE 8

Note: the improvement rate (%) (base value-N day test value using mask)/base value × 100%.

The results show that the skin percutaneous water loss rate is remarkably reduced after 14 days and 28 days of using the facial mask disclosed by the invention, and the improvement rate is 7.97% and 7.64% respectively.

2) The results of the skin oil content test are shown in table 9 and fig. 2.

TABLE 9

Note: the improvement rate (%) (base value-N day test value using mask)/base value × 100%.

The results show that the skin oil content is remarkably reduced after 14 days and 28 days of using the facial mask disclosed by the invention, and the improvement rates are respectively 2.03% and 8.9%.

3) The skin melanin value (pox mark) test results are shown in table 10 and figure 3.

Watch 10

Note: the improvement rate (%) (base value-N day test value using mask)/base value × 100%.

The results show that the skin melanin value (acne mark) is remarkably reduced after 14 days and 28 days of using the facial mask disclosed by the invention, and the improvement rate is 4.95% and 10.37% respectively.

4) The results of the skin erythrocin value (inflammatory papules) test are shown in table 11 and figure 4.

TABLE 11

Note: the improvement rate (%) (base value-N day test value using mask)/base value × 100%.

The results show that the skin erythrocin value (inflammatory papules) is remarkably reduced after 14 days and 28 days of using the facial mask disclosed by the invention, and the improvement rate is 8.12% and 15.79% respectively.

5) The red zone area ratio test results are shown in table 12 and fig. 5 and 6.

TABLE 12

Note: the improvement rate (%) (base value-N day test value using mask)/base value × 100%.

The results show that the red area ratio is remarkably reduced after 14 days and 28 days by using the mask disclosed by the invention, and the improvement rate is 20.06% and 54.66% respectively.

6) The results of the stratum corneum cell exfoliation test are shown in table 13 and fig. 7.

Watch 13

Note: the improvement rate (%) (base value-N day test value using mask)/base value × 100%.

The results show that the exfoliating of the skin cuticle cells is obviously reduced after 14 days and 28 days of the mask of the invention, and the improvement rate is 10.08 percent and 19.40 percent respectively.

7) The dermatologist evaluation FENC is shown in table 14 and fig. 8 and 9.

TABLE 14

Note: the improvement rate (%) (base value-N day test value using mask)/base value × 100%.

The results show that the skin damage (inflammatory pimple + open acne + closed acne) is obviously improved after 14 days and 28 days of using the facial mask provided by the invention, and the improvement rate is 8.87% and 24.81% respectively.

8) The subject self-evaluation results are shown in table 15.

Watch 15

	Feeling after using the product	D14 satisfaction degree	D28 satisfaction degree
				1	The product has reduced greasy feeling on facial skin	93.9％	96.97％
2	The product can smooth out the initial pox	84.8％	96.97％
				3	The product can relieve acne area discomfort	72.7％	96.97％
4	The face acne is obviously reduced	72.7％	96.97％
				5	The acne on face is obviously improved	78.8％	93.94％
6	The product has no tight feeling after being used	87.9％	100.00％
				7	Has no irritation after using the product	93.9％	100.00％
8	The product has no redness after being used	97.0％	100.00％
				9	Product mildness	97.0％	100.00％
10	Whether you want to go on to buy the product again		100% will like

Items 1 to 5: score 1 indicates very different agreement, score 2 indicates comparatively different agreement, score 3 is general, score 4 indicates comparatively identical agreement, and score 5 indicates very different agreement.

Items 6 to 9: score 1 indicates very poor, score 2 indicates poor, score 3 is normal, score 4 indicates good, and score 5 indicates very good. Item 10: score 1 indicates yes and score 2 indicates no.

Items 1 to 5: d14 satisfaction and D28 satisfaction were reported as the percentage of "4 out of the better agreement" and "5 out of the better agreement" after 14 and 28 days of product use.

Items 6 to 9: d14 satisfaction and D28 satisfaction were percentages of "better than 4" and "very good than 5" after 14 and 28 days of product use.

Item 10: d28 satisfaction was the percentage of "2 points willing" after 28 days of product use.

To summarize:

the detection result shows that the mask disclosed by the invention can obviously improve the skin percutaneous water loss, has the effects of controlling oil and fading acne marks, and obviously improves the skin problems of inflammatory pimples, skin redness, cuticle cell peeling, skin damage and the like by using the mask disclosed by the invention for 28 consecutive days by 33 healthy male and female subjects in China. The subjective evaluation results showed that: the whole using feeling is better, and after 28 days of use, the comprehensive evaluation of the testee is satisfied with the mask of the invention.

Example 8 toxicology testing of masks of the invention

First, acute eye irritation/Corrosion test

1. The test substance: the mask samples described in example 5.

2. Animal and feeding environment: japanese big ear white rabbit, male, general grade, provided by Beijing gold shepherd Yang laboratory animal Breeding, Inc., license number: SCXK (Kyoto) 2020 + 0002, 3 in total, the body weight is 2.6-2.9 kg. Animals were acclimated in a general environment animal room for 3 days and the test was performed after confirming the health of the animals. The temperature of the animal room is 20-26 ℃, the relative humidity is 40-70%, and the light and the shade alternate after 12 hours of illumination. The animal feed is purchased from Beijing, Australian cooperative feed Co., Ltd, license number SCXK (Jing) 2019-. The drinking water is tap water. License number for experimental animals: SYXK (Kyoto) 2018-.

3. The test method comprises the following steps: both eyes of the animals were examined 24 hours before the test, and the animals selected were all free from eye irritation symptoms, corneal defects and conjunctival lesions. 0.1mL of the test object is dripped into the animal left eye conjunctival sac, the upper eyelid and the lower eyelid are passively closed for 1 second, the test object is dripped into the test object for 24 hours without flushing, and the right eye is not treated and is used as self control. The eyes of the animals were examined at 1 hour, 24 hours, 48 hours, and 72 hours after instillation of the test substance, and the eyes of all animals were further examined with sodium fluorescein at the time of 24 hours examination. The test is scored according to the scoring standard in the "acute eye irritation/corrosiveness test" of technical Specification for safety of cosmetics (2015 edition), and the eye irritation intensity of the test object is determined based on the highest integral mean value and recovery time evaluation of the irritation response of the cornea, iris or conjunctiva of the animal at the observation time points of 24 hours, 48 hours or 72 hours after the test object is given.

4. And (4) conclusion: test results show that the mask provided by the invention can be used for treating white eyes without eyes of Japanese big ears under the condition of no flushing

And (4) irritation.

Two or more skin irritation/Corrosion tests

1. The test substance: the mask samples described in example 5.

2. Animal and feeding environment: japanese big ear white rabbit, male, general grade, provided by Changyang west mountain farm in Beijing city, license number: SCXK (Jing) 2016-. The animals are adapted to the ordinary environment animal room of the unit for 3 days, animal quarantine is completed, and the test is carried out after the animal health is confirmed. The temperature of the animal room is 20-26 ℃, the relative humidity is 40-70%, and the light and the shade alternate after 12 hours of illumination. The animal feed is purchased from Beijing, Australian cooperative feed Co., Ltd, license number SCXK (Jing) 2019-. License number for experimental animals: SYXK (Kyoto) 2018-.

3. The test method comprises the following steps: 24 hours before the test, the two side hairs of the spine of the animal are removed, 0.5mL of the test object is taken from the left side and the right side of the hair removing range of 3cm multiplied by 3cm., the test object is smeared on the skin on the left side, the smearing range is 2.5cm multiplied by 2.5cm, the test object is smeared for 1 time every day, the smearing is continuously carried out for 14 days, and the skin on the right side is taken as a control. Starting on the next day, the hair was removed before each application and washed with warm water to remove residual test substance. After 1 hour, the results were observed and scored according to the technical Specification for cosmetic safety (2015 edition), and the control and test areas were treated in the same manner.

After the experiment is finished, the average integral of each animal every day is calculated according to the following formula, and the skin irritation intensity is judged according to the highest integral average value.

4. And (4) conclusion: test results show that the facial mask provided by the invention has no irritation to repeated skin irritation of Japanese white rabbits with big ears.

Example 924 h skin occlusion Patch test

The test method comprises the following steps: refer to technical Specifications for cosmetic safety (2015 edition).

The test substance: the mask according to example 5 of the present invention.

Negative control: and (5) a filter disc.

Subject completion status: a total of 32 subjects (of which 1 was withdrawn) were enrolled and finally 31 subjects completed the trial, 7 of which were male, 24 female, and aged 22-60 years.

The qualified spot test device with the area not more than 50mm2 and the depth of about 1mm is selected. The test substance is added to the plaque test device in an amount of about 0.020g to about 0.025g (solid or semi-solid) or 0.020mt to about 0.025mL (liquid). The patch test device with the test substance and the negative control was applied to the curved side of the forearm of the subject, and the patch test device was removed 24 hours later. Skin reactions were observed according to the standard of table 16 at 30 minutes (after disappearance of indentation), 24 hours, and 48 hours after removal of the test article plaque test device, respectively, and the observation results were recorded.

TABLE 16 skin closed type class Patch test skin response grading Standard

The test results are shown in table 17. The test results show that no skin reaction occurs in 31 subjects at 0.5h,24h and 48h after the test object spot tester is removed, and the grading grades are all 0.

TABLE 17 skin Enclosed Patch test results