阅读说明：本技术 一种减重组合物及其在制备减肥产品中的应用 (Weight-reducing composition and application thereof in preparation of weight-reducing product ) 是由 高江涛 邵明亮 梁馨文 刘建明 于 2021-09-01 设计创作，主要内容包括：本发明属于功能食品技术领域,提供了一种减重组合物及其在制备减肥产品中的应用,减重组合物包括植物多酚和益生元,所述植物多酚和益生元的质量比为1:9～9:1。所述植物多酚为虎杖提取物、接骨木莓提取物、欧洲越橘提取物、菝葜多酚、巴西莓提取物中的一种或两种。所述益生元为低聚甘露糖、低聚木糖、低聚半乳糖、水苏糖中的任意两种或三种益生元组合。本发明针对无法定向调节肠道内瘦子菌这一技术问题,将几种植物多酚与益生元进行结合,在增殖肠道瘦子菌的同时,也对胖子菌的生长有抑制作用,实现肠道内微生态的定向调控。通过天然来源的益生元和植物多酚对肠道菌群进行调控,使得机体实现良好减重效果。(The invention belongs to the technical field of functional foods and provides a weight-reducing composition and application thereof in preparing a weight-reducing product, wherein the weight-reducing composition comprises plant polyphenol and prebiotics, and the mass ratio of the plant polyphenol to the prebiotics is 1: 9-9: 1. The plant polyphenol is one or two of rhizoma Polygoni Cuspidati extract, herba Sambuci Williamsii extract, Vaccinium myrtillus extract, rhizoma Smilacis chinensis polyphenol, and Brazil berry extract. The prebiotics are any two or three of oligomannose, xylo-oligosaccharide, galacto-oligosaccharide and stachyose. Aiming at the technical problem that the lean bacterial in the intestinal tract can not be directionally regulated, the invention combines a plurality of plant polyphenols and prebiotics, so that the growth of the fat seed bacteria is inhibited while the intestinal tract lean bacterial is proliferated, and the directional regulation of the micro-ecology in the intestinal tract is realized. The intestinal flora is regulated and controlled by prebiotics and plant polyphenol which are natural sources, so that the body can realize good weight loss effect.)

1. A weight reducing composition characterized by: the plant polyphenol and prebiotics are contained, and the mass ratio of the plant polyphenol to the prebiotics is 1: 9-9: 1.

2. A weight reducing composition according to claim 1, wherein: the plant polyphenol is one or two of rhizoma Polygoni Cuspidati extract, herba Sambuci Williamsii extract, Vaccinium myrtillus extract, rhizoma Smilacis chinensis polyphenol, and Brazil berry extract.

3. A weight reducing composition according to claim 1, wherein: the prebiotics are any two or three of oligomannose, xylo-oligosaccharide, galacto-oligosaccharide and stachyose.

4. A weight reducing composition according to claim 2, wherein: the purity of a single plant polyphenol is not less than 20%, and the mass ratio of any two plant polyphenols is 1: 9-9: 1.

5. A weight reducing composition according to claim 3, wherein: the content of single prebiotics is not less than 85%, the weight ratio of any two prebiotics is 1: 9-9: 1, the weight ratio of any three prebiotics is 1:1: 8-8: 1: 1.

6. Use of a weight reducing composition according to any one of claims 1-5 in the manufacture of a weight reducing product.

7. Use of a weight reducing composition according to claim 6 in the preparation of a weight reducing product, wherein: the weight-reducing product is a food, a beverage or a pharmaceutical preparation.

8. Use of a weight reducing composition according to claim 7 in the manufacture of a weight reducing product, wherein: the medicinal preparation is soft capsule, pill or microcapsule.

Technical Field

The invention belongs to the technical field of functional foods, and particularly relates to a main weight-reducing composition and application thereof in preparing a weight-reducing product.

Background

With the development of economic society and the improvement of the living standard of people, the incidence rate of obesity also increases year by year. Prevention and treatment of obesity has become a major health challenge facing modern society. In recent years, numerous studies have shown that the intestinal microflora is closely associated with obesity. The Jeffrey Gordon teaches that intestinal bacteria of fat mice and intestinal bacteria of another lean mice with characteristic abundance ratio are transplanted into the intestinal tract of sterile mice, and the surprising discovery that the fat weight of the sterile mice transplanted with the intestinal bacteria of the fat mice is increased by 20 percent compared with the fat weight of the sterile mice transplanted with the intestinal bacteria of the lean mice after two weeks shows that the inevitable relationship exists between the intestinal flora and the obesity.

The specific species of the intestinal flora associated with obesity can be broadly classified into lean-daughter bacteria and fat-daughter bacteria according to their effects on the host. Leptospirillum is a group of beneficial bacteria that improve obesity by enhancing metabolic absorption in the host. Mainly comprises Bacteroides (A), (B)Bacteroidetes) Bifidobacterium (b)Bifidobacterium) Genus Ackermansia (a)Akkermansia) Mainly, etc., whereinAkkermansia muciniphilaThe effect of abundance of Bacteroides thetaiotaomicron (Ikemansia) and Bacteroides thetaiotaomicron (Bacteroides) on obesity is particularly significant.Akkermansia muciniphilaThe bacteria have good regulating effect on hyperglycemia and obesity by regulating and controlling the thickness of mucus in the intestinal tract, maintaining the integrity of intestinal tract barriers and other regulating effects on endocrine; bacteroides thetaiotaomicron is especially useful in improving nutrient availability and polysaccharide utilization. The fat bacteria mainly comprise Desulfurvibrio (B)Desulfovibrio) Enterobacter (a)Enterobacter) And the like, which are harmful flora capable of secreting endotoxin and causing inflammation and metabolic problems to the body. Such as Enterobacter cloacae (Enterobacter) can activate inflammation and insulin resistance in mice, activate genes that synthesize fat, and cause obesity in hosts.

Numerous studies currently focus on prebiotic proliferation of leptospirillumAkkermansia muciniphilaBacteroides thetaiotaomicron and bifidobacterium to enable the host to achieve the weight reduction effect. However, because the affinity of most intestinal bacteria to common prebiotics is similar, when one prebiotic is used, the proliferation of the lean germ group and the proliferation of the fat germ group can be caused, and the rapid obesity can be caused. Therefore, the search for a composition capable of directionally proliferating the growth of the leptospirillum is an important research direction for improving the intestinal flora.

Disclosure of Invention

The object of the present invention is to provide a weight reducing composition which overcomes the above mentioned technical problems of the prior art.

The invention also aims to provide the application of the weight-reducing composition in preparing a weight-reducing product, and the weight-reducing composition can improve intestinal flora to ensure that a host achieves a good weight-reducing effect.

Therefore, the technical scheme provided by the invention is as follows:

a weight-losing composition comprises plant polyphenol and prebiotics, wherein the mass ratio of the plant polyphenol to the prebiotics is 1: 9-9: 1.

The plant polyphenol is one or two of rhizoma Polygoni Cuspidati extract, herba Sambuci Williamsii extract, Vaccinium myrtillus extract, rhizoma Smilacis chinensis polyphenol, and Brazil berry extract.

The prebiotics are any two or three of oligomannose, xylo-oligosaccharide, galacto-oligosaccharide and stachyose.

The purity of a single plant polyphenol is not less than 20%, and the mass ratio of any two plant polyphenols is 1: 9-9: 1.

The content of single prebiotics is not less than 85%, the weight ratio of any two prebiotics is 1: 9-9: 1, the weight ratio of any three prebiotics is 1:1: 8-8: 1: 1.

Application of a weight reducing composition in preparing weight reducing product is provided.

The weight-reducing product is a food, a beverage or a pharmaceutical preparation.

The medicinal preparation is soft capsule, pill or microcapsule.

The invention has the beneficial effects that:

the weight-reducing composition provided by the invention aims at the technical problem that the lean bacterial in the intestinal tract can not be directionally regulated, combines a plurality of plant polyphenols and prebiotics, has an inhibiting effect on the growth of the fat seed bacteria while proliferating the intestinal tract lean bacterial, and realizes the directional regulation of the micro-ecology in the intestinal tract. The intestinal flora is regulated and controlled by prebiotics and plant polyphenol which are natural sources, so that the body can realize good weight loss effect.

The plant polyphenol and prebiotics combination can be used as a safe and harmless weight-losing functional component and can be used in various end products such as liquid beverages, solid beverages, meal replacement bars, candies, fermented milk and the like.

The following will be described in further detail with reference to the accompanying drawings.

Drawings

FIG. 1 is an in vitro effect of prebiotics and plant polyphenols on lean germ;

FIG. 2 is an in vitro effect of prebiotics and plant polyphenols on Scaphius sp;

FIG. 3 is a graph of the in vitro effect of different example weight loss compositions on leptospira;

FIG. 4 is a graph of the in vitro effect of different example weight loss compositions on Sterculia adiposa

Figure 5 is the effect of the weight loss composition of example 2 on the rate of weight gain in mice;

figure 6 example 2 profile of the effect of weight loss composition on intestinal flora in mice.

Detailed Description

The following description of the embodiments of the present invention is provided for illustrative purposes, and other advantages and effects of the present invention will become apparent to those skilled in the art from the present disclosure.

The exemplary embodiments of the present invention will now be described with reference to the accompanying drawings, however, the present invention may be embodied in many different forms and is not limited to the embodiments described herein, which are provided for complete and complete disclosure of the present invention and to fully convey the scope of the present invention to those skilled in the art. The terminology used in the exemplary embodiments illustrated in the accompanying drawings is not intended to be limiting of the invention. In the drawings, the same units/elements are denoted by the same reference numerals.

Unless otherwise defined, terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Further, it will be understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the relevant art and will not be interpreted in an idealized or overly formal sense.

Example 1:

the embodiment provides a weight-losing composition, which comprises plant polyphenol and prebiotics, wherein the mass ratio of the plant polyphenol to the prebiotics is 1: 9-9: 1.

Wherein the plant polyphenol is one or two of rhizoma Polygoni Cuspidati extract, herba Sambuci Williamsii extract, Vaccinium myrtillus extract, rhizoma Smilacis chinensis polyphenol, and Brazil berry extract.

The prebiotics are any two or three of oligomannose, xylo-oligosaccharide, galacto-oligosaccharide and stachyose.

The mechanism of the invention is as follows:

by combining a plurality of plant polyphenols with prebiotics, the growth of the fat seed bacteria is inhibited while the intestinal lean seed bacteria are proliferated, and the directional regulation and control of the micro-ecology in the intestinal tract are realized. The intestinal flora is regulated and controlled by prebiotics and plant polyphenol which are natural sources, so that the body can realize good weight loss effect.

Example 2:

on the basis of example 1, the present example provides a weight-reducing composition, which includes plant polyphenol and prebiotics at a mass ratio of 1: 1.

In the embodiment, the plant polyphenol is sarsaparilla polyphenol and acai berry extract, and the weight parts of the plant polyphenol are 20 parts; the prebiotics are low polymannan and stachyose, and the weight parts of the prebiotics are 20 parts.

Example 3:

on the basis of example 1, the present example provides a weight-reducing composition, which includes plant polyphenol and prebiotics at a mass ratio of 6: 1.

In the embodiment, the plant polyphenol is sarsaparilla polyphenol and acai berry extract, and the weight parts are 10 parts and 20 parts respectively; the prebiotics are oligomannose and stachyose, and the weight parts are 3 parts and 2 parts respectively.

Example 4:

on the basis of example 1, the present example provides a weight-reducing composition, which includes plant polyphenol and prebiotics at a mass ratio of 1: 8.

In the embodiment, the plant polyphenol is sarsaparilla polyphenol and acai berry extract, and the weight parts are 3 parts and 2 parts respectively; the prebiotics are low polymannan and stachyose, and the weight parts of the prebiotics are 20 parts.

Example 5:

on the basis of example 1, the present example provides a weight-reducing composition, which includes plant polyphenol and prebiotics at a mass ratio of 3: 8.

In this example, the plant polyphenol is elderberry extract and vaccinium myrtillus extract, 10 parts and 5 parts by weight respectively; the prebiotics are galacto-oligosaccharide and stachyose, and the weight parts are 3 parts and 2 parts respectively.

In-vitro test for respectively researching influence of prebiotics and plant polyphenol on intestinal lean seed bacteria and fat seed bacteria

1. Prebiotic materials for testing

Oligomannose (85%, produced by west Annoy health Biotechnology, Inc.), Longli xylooligosaccharide (95%, Shandong Longli Biotechnology, Inc.), stachyose (94.17%, Xian Tianmei Biotechnology, Inc.), and galactooligosaccharide (90%, Shandong Bailong Chuang Biotechnology, Inc.).

2. Test strains

Lean bacterial: bacteroides thetaiotaomicron: (Bacteroides thetaiotaomicronATCC 29148) Ackermanella muciniphila (Akkermansia muciniphila 、CICC24917）

C, boat-fruited sterculia seed bacteria: bacillus desulphurosusDesulfovibrio desulfuricans subsp. DesulfuricansATCC 7757), Enterobacter cloacae: (Enterobacter cloacae、 CICC10450）

Lean and fat bacteria are purchased from China center for culture Collection of microorganisms and China institute of food fermentation industry.

3. Culture medium

BHI Medium (BHI)Akkermansia muciniphila、Bacteroides thetaiotaomicron basal medium): 12.5g of dehydrated calf brain extract powder, 5g of dehydrated calf heart extract powder, 10.0g of peptone, 2.0g of glucose, 20.0g of agar, 2g of mucin and 1000ml of distilled water, wherein the pH value is 6.8-7.4

Nutrient agar medium (basic medium for vibrio desulfurizati and enterobacter cloacae): 10.0g of beef extract, 10.0g of peptone, 10.0g of glucose, 5.0g of NaCl, 15.0-20.0 g of agar, 1.0L of distilled water and pH7.0

Carbon-deficient culture medium: removing a carbon source from a basic culture medium corresponding to the strain to obtain a carbon-deficient culture medium;

treatment group medium: the corresponding treated group medium was prepared by adding 2% by weight of the test substance to the carbon-deficient medium.

4. Influence experiment:

respectively inoculating the cultured primary bacterial suspension into a basic culture medium, a carbon-deficient culture medium and a treatment group culture medium according to the inoculation amount of 3-5%, wherein the liquid loading amount of the culture medium is 90% of the volume of the test tube, sealing, and performing constant-temperature standing culture in an anaerobic bag incubator at 37 ℃.

5. Bacterial growth assay

After 48h incubation, the OD of the cells was measured at a wavelength of 600nm using a spectrophotometer.

6. Results of the experiment

1) The proliferation effect of single prebiotics, prebiotic combinations, single polyphenols and polyphenol combinations on lean seeds is shown in figure 1: (1) in terms of prebiotics, stachyose and oligomannose have a significant proliferative effect on leptospirillum relative to other prebiotics. And after the two prebiotics with better proliferation effects are compounded, the proliferation effect can be further improved. The proliferation effect of the composite prebiotics on Akkermansia muciniphila is improved by 25.86 percent compared with the single prebiotics with the optimal proliferation effect (the low polymannan mannose is used as a carbon source for culturing for 48h, and the OD value is 0.719), and the proliferation effect on the bacteroides thetaiotaomicron is improved by 42.38 percent compared with the single prebiotics with the optimal proliferation effect (stachyose is used as a carbon source for culturing for 48h, and the OD value is 0.512); (2) in the aspect of plant polyphenol, most of single polyphenols have no obvious proliferation effect on the lean bacteria, and the smilax polyphenol and the acai berry in the polyphenol composition have a certain proliferation effect on the lean bacteria, but the proliferation effect is not obvious compared with the combined proliferation effect of prebiotics.

2) The effect of single prebiotics, prebiotic combinations, single polyphenols and polyphenol combinations on the sterculia sp, results are shown in figure 2: (1) different prebiotics and prebiotic combinations have certain proliferation effects on fat bacterium vibrio desulphurizing bacillus and enterobacter cloacae, wherein the proliferation effect of galactooligosaccharides is most obvious; (2) the plant polyphenol has no proliferation effect on the fat bacteria, wherein the combination of the smilax polyphenol and the acai berry has a certain inhibition effect on the fat bacteria. Compared with the growth amount of the boat-fruited fruit fungi in the blank control (the OD value of the enterobacter cloacae cultured for 24h is 0.335, and the OD value of the vibrio desulfurated for 24h is 0.303), the growth amount of the enterobacter cloacae cultured by taking the sarsaparilla polyphenol and the babi berry as carbon sources is reduced by 69.85%, the growth amount of the vibrio desulfurated is reduced by 58.08%, and the inhibition effect is obvious.

In conclusion, the prebiotics have obvious proliferation effect on the leptospirillum, the plant polyphenol has no proliferation effect on the stersporum, and part of polyphenol combination can inhibit the stersporum. Plant polyphenol and prebiotics can be organically combined to adjust the proportion of lean seed bacteria and fat seed bacteria in intestinal tract.

Second, efficacy evaluation of prebiotic and polyphenol weight loss composition

1. In vitro evaluation of weight loss compositions

The weight-loss compositions of examples 2-5 were evaluated for in vitro effects, respectively, wherein the combinations of example 2 (mannose oligomer: stachyose: sarsaprophenol: acai berry extract =20: 20: 20: 20) had significant effects on the proliferation of leptin (see fig. 3), increased by 112.37% on Akkermansia muciniphila compared to "mannose oligomer + stachyose" and 87.10% on bacteroides thetaiotaomicron compared to "mannose oligomer + stachyose". (ii) a

The weight-reducing composition of example 2 also had a good inhibitory effect on obese bacteria. As shown in figure 4, the inhibition effect on enterobacter cloacae is improved by 37.81% compared with that on "smilax polyphenol + acai berry", and the inhibition effect on vibrio desulphurized is improved by 51.82% compared with that on "smilax polyphenol + acai berry".

It can be seen that by combining prebiotics and plant polyphenols according to the formulation of example 2, not only the proliferation effect of prebiotic combination on lean bacterial cells is improved, but also the inhibition effect on fat bacterial cells is improved. The prebiotics and the polyphenol have obvious effect on the directional proliferation of the leptospirillum, the growth amount of beneficial groups in a community is increased, the nutrition acquisition of the growth of the leptospirillum is indirectly hindered, and the inhibition effect is further expanded.

1. Effect of weight loss composition on mouse body weight and intestinal flora

Based on the above test results, the in vivo effect was evaluated by conducting a mouse intestinal flora influence experiment on the weight-loss composition of example 2 screened in vitro. The specific flow is as follows

(1) Laboratory animal

C57BL/6J mice, were housed under SPF-class conditions, with no more than 6 mice housed per cage. The mice are raised in a strictly controlled environment, the normal circadian rhythm is maintained, the day time is 12 hours and the night time is 12 hours, the light-off time is 7 o' clock at night,

(2) experiment grouping

48 male C57BL/6J mice, 5 weeks old, were picked up and acclimatized for one week, during which time sufficient water and normal diet were given. One week later, all mice were randomly divided into four groups, one group was fed with a high fat diet containing 45% fat as HFD/(-) group, and the other three groups were fed with 3g of oligosaccharide and stachyose composition (1: 1 by weight), 3g of sarsaprophenol + bayberry (1: 1 by weight), and 3g of prebiotic composition of example 2 as "oligomannose + stachyose", "sarsaprophenol + bayberry", and HFD/(example 2) group, respectively. Four groups of mice were exposed to unlimited water and food. After 12 weeks of feeding, the fecal flora structure of the mice was analyzed and the weight changes of the mice were monitored during the feeding period.

(3) Mouse feces collection

Preparing EP tubes sterilized by high-temperature steam, sterile cotton swabs, ice boxes, marking pens, gloves and the like.

The mice were grabbed, left-hand baoding (held stationary), and right-hand EP tubes were held to collect the mouse feces. The hair of the mice is avoided. If the mouse is not defecation in time, conduct gentle Rou on the abdomen of the mouse with the index finger of the right hand, and wait for a moment.

Feces were collected, labeled, immediately placed on ice, and frozen at-80 ℃ for 1 hour until fecal DNA extraction was performed.

(4) Fecal DNA extraction

Preparing a sterilized 2mLEP tube, preparing an ice box, and preheating a water bath kettle at 70 ℃.

180-220mg of feces were weighed per sample and placed on ice.

Add 1mL of InhibitEXBuffer to each sample and continue vortex for 1min until the samples are completely mixed. Bulk samples can be broken up manually with the aid of a 200 μ L pipette tip.

Water bath at 70 deg.C for 5min, and centrifuging for 15 s.

The solid particles in the feces were precipitated by centrifugation at full speed (20,000 Xg, hereinafter all at full speed) for 1 min.

Aspirate 15. mu.L of proteinase K into a new EP tube.

Pipetting 600. mu.L of the supernatant from step (5) into an EP tube containing proteinase K. (8) Add 200. mu.L of Bufferal, vortex for 15 s.

Water bath at 70 deg.C for 10min, and centrifuging for 15 s.

Add 200. mu.L of absolute ethanol and vortex well.

The lysates were carefully added to a QIAamp spin column, capped, and centrifuged for 1 min. The collection tube is discarded and replaced with a new collection tube.

The lid of the spin column was carefully opened, and 500. mu.L of BufferAW1 was added and the mixture was centrifuged for 1min for washing. The collection tube is discarded and replaced with a new collection tube.

The lid of the spin column was carefully opened, and 500. mu.L of BufferAW2 was added and the mixture was centrifuged for 1min for washing. The collection tube is discarded and replaced with a new collection tube.

Carefully open the spin column lid and then close it to volatilize the liquid. The collection tube was discarded and replaced with a new one and centrifuged for 3min to remove the participating liquid.

Place the column in a labeled EP tube, add 200. mu.L of buffer ATE to the membrane, incubate for 1min at room temperature, and centrifuge for 1 min.

The DNA concentration and purity were determined by Nanodrop.

DNA size and degradation were checked by 1% agarose gel electrophoresis (180V, 18 min).

(5) 16S rRNA amplicon sequencing and analysis

The DNA samples were sent to Conbo Biotech, Beijing for 16SrRNA gene amplicon sequencing.

The gene universal primers 515F (5' -GTGCCAGCMGCCGCGGTAA-3 and 806R (5' -GGACTACHVGGGTWTCTAAT-3') amplify the V4 region of the 16srRNA gene, DNA agarose gel electrophoresis, gel recovery and purification are carried out, and the purity concentration of DNA is detected.

Direct sequencing linker addition by TruSeqDNAPCR-FreeSamplePreparation kit, without PCR amplification, to construct sequencing library

Sequencing company analyzes the raw data according to the bar code of the sequencing sample

(6) Results of the experiment

As shown in figure 5, mice were fed "oligomannose + stachyose" and HFD/(example 2) with significantly lower body weight growth rates during feeding than the unfed groups HFD/(-) and "sarsaprophenol + bacon". After 12 weeks, the average body weight of the HFD/(example 1) group mice was reduced by about 23.2% compared to the HFD/(-) group. The composition of example 2 had a significant weight loss effect in mice, as shown in table 1.

Table 1 example 2 effects on body weight of mice

Distribution of intestinal flora

The results of the detection of the mouse intestinal flora are shown in FIG. 6, and compared with HFD/(-) -, "oligomannose + stachyose" and "sarsaprophenol + bacon berry", the results of the detection of the mouse intestinal flora in HFD/(example 2) mouse intestinal flora are shown in FIG. 6Bacteroides、AkkermansiaIs raised in whichAkkermansiaThe increase is most obvious, and the relative abundance is increased from 4% to 26%; sterculia scaphigeraDesulfovibrioThe relative abundance is reduced from 4% to below the 1% level. The composition in example 2 has obvious effect of regulating the ratio of intestinal lean bacteria to intestinal fat bacteria in high-fat diet mice.

2. Determination of weight-reducing and fat-reducing effects of weight-reducing composition on human body

Control group: 20 persons are respectively 10 men and 10 women, and have normal diet; experimental groups: total 60 persons, divided into 3 groups of 20 persons each (10 men, 10 women). In addition to the normal diet, 3g of oligosaccharide and stachyose composition (1: 1 by weight), 3g of sarsaparillo + bayberry (1: 1 by weight), and 3g of prebiotic composition of example 2, which was recorded as "oligomannose + stachyose", "sarsaparillo + bayberry" and HFD/(example 2) were taken before lunch every day.

Days of the experiment: 30 days; the weight loss and fat loss were measured after 30 days, and the measurement data are shown in Table 2.

Table 2 example 2 test of weight loss and fat loss effects in human body

The experimental results show that the weight loss rate of the group "HFD/(example 2)" is about 6% and the fat loss rate is about 3% for the same food intake, and the group has significant differences compared with the group "mannose oligomer + stachyose", "sarsaprophenol + acai berry" and the control group. The prebiotic composition of HFD/(example 2) group has good weight-reducing and fat-reducing effects in human body.

The above examples are merely illustrative of the present invention and should not be construed as limiting the scope of the invention, which is intended to be covered by the claims and any design similar or equivalent to the scope of the invention.