阅读说明：本技术 一种应用于生物降解试验的测试系统 (Testing system applied to biodegradation test ) 是由 张乐 苏来 于 2020-10-16 设计创作，主要内容包括：本发明公开了一种应用于生物降解试验的测试系统,包括CO-(2)去除单元和反应单元,反应单元包含反应瓶和CO-(2)收集瓶,所述反应瓶下方安装有磁力搅拌器,所述反应瓶内含有一个转子；CO-(2)去除单元包括有一个气泵,所述气泵与二级填料柱相连接,所述填料柱之间通过导气管相连接,填料柱内有足量脱脂棉,所述填料柱由固定装置固定,本发明集成了CO-(2)去除单元和反应单元,系统简单,可操作性强,便于实验室内自己组装,为用于评估新化学物质生物降解试验(CO-(2)产生法)提供便利。(The invention discloses a testing system applied to a biodegradation test, which comprises CO 2 A removal unit and a reaction unit, the reaction unit comprising a reaction flask and CO 2 The magnetic stirrer is arranged below the reaction bottle, and the reaction bottle is internally provided with a rotor; CO 2 2 The removing unit comprises an air pump, the air pump is connected with two stages of packing columns, the packing columns are connected through air guide tubes, sufficient absorbent cotton is arranged in the packing columns, and the packingThe column is fixed by a fixing device, and the invention integrates CO 2 The removal unit and the reaction unit have simple system and strong operability, are convenient to assemble in a laboratory and are used for evaluating the biodegradation test (CO) of new chemical substances 2 Production method) provides convenience.)

1. A testing system for biodegradation test comprises CO₂A removal unit (2), the CO₂The air pump contained in the removing unit (2) conveys air to a secondary filling column (13), absorbent cotton (9) is filled above and below the secondary filling column (13), the secondary filling column (13) and the absorbent cotton (9) are separated by a sieve plate (10) to ensure that the secondary filling column is not polluted, the two secondary filling columns (13) are fixed by a fixing device (11), an air guide pipe is connected above the two secondary filling columns (13), a fixing knob (8) is arranged between the two secondary filling columns (13) to control the fixing degree, and the air is conveyed to be filled with Ba (OH) after the reaction of the secondary filling columns (13)₂The water washing bottle and the water-containing water washing bottle, the water-containing water washing bottle conveys the gas after reaction into the reaction unit (1) after the flow of the gas is controlled by the flowmeter, and the reaction unit (1) comprises CO₂An absorption bottle (3), a reaction bottle (4), a magnetic stirrer (5) arranged below the reaction bottle (4), and a reaction tankA stirrer (6) is arranged in the reaction bottle (4), and a feeding pipe (7) with a cover is arranged above the reaction bottle.

2. The system of claim 1, wherein the CO is selected from the group consisting of₂The absorption bottles (3) are divided into an upper group and a lower group, the middle groups are connected by an air duct, and the upper group is CO₂The absorption bottle (3) can carry out absorption for 2 times.

3. The testing system for biodegradation tests according to claim 1, characterized in that said reaction flask (4) is equipped with said capped feeding tube (7) to allow feeding without opening the flask.

4. The testing system applied to the biodegradation test according to claim 1, characterized in that the secondary packing column (13) is filled with porous soda lime which can sufficiently absorb CO in air₂And water.

5. The testing system for biodegradation tests according to claim 1, characterized in that said reaction flask (4) contains the reaction suspension (microorganisms, test substances) required by the test, and the contents are kept in suspension by said magnetic stirrer (5) and said stirrer (6).

Technical Field

The invention relates to the field of biodegradation tests, in particular to a test system applied to a biodegradation test.

Background

Biodegradation means that the material is continuously discharged from the body in the process of tissue growing in through the actions of dissolution, enzymolysis, cell phagocytosis and the like in the body, and the repaired tissue completely replaces the position of the implanted material, so that the material has no residual property in the body.

The process of biodegradation can be divided into three stages: biodegradation, biological disruption and absorption. Biodegradation is sometimes described as surface level degradation, changing the mechanical, physical and chemical properties of the material, which occurs when the material is exposed to non-biological factors in an outdoor environment, and causing further degradation of the material by weakening its structure. Some abiotic factors influencing these initial changes are usually atmospheric pressure, light intensity, temperature and chemical substances in the environment, although biological disruption usually occurs in the decomposition stage of the biodegradation process, but may be parallel to the biodegradation in some cases, and then products generated by the biodegradation are integrated into microbial cells in the absorption stage, some products generated by the disintegration are easy to transport in the cells through membrane carriers, but other compounds still must carry out biotransformation reaction to generate products which can be transported in the cells, because the biodegradable materials are extracted from the organisms without depending on petroleum, and can be decomposed automatically after being discarded, thereby reducing the pollution to the environment, following the current trend of global product environmental protection, meeting the requirements of most countries with serious environmental pollution for environment governance, when various biodegradable materials are chemically reacted with respective substrates, no generation of redundant pollutants, positive and positive effect on environmental condition control,

the existing testing system for biodegradation tests cannot accurately control CO₂The gas content absorption degree, and need frequently uncap the filler, cause the gas to receive the pollution to reduce test system's work efficiency, and reduced test system's test accuracy.

Disclosure of Invention

The present invention is directed to a testing system for biodegradation tests, which solves the above problems of the prior art.

In order to achieve the purpose, the invention provides the following technical scheme: a test system applied to biodegradation test comprises CO₂Removal unit and reactionA reaction unit comprising a reaction flask and CO₂The magnetic stirrer is arranged below the reaction bottle, and the reaction bottle is internally provided with a rotor; CO 2₂The removing unit comprises an air pump, the air pump is connected with two stages of packing columns, the packing columns are connected through air guide tubes, sufficient absorbent cotton is arranged in the packing columns, the packing columns are fixed by a fixing device, are suspended above the air pump and then are washed by water (used for absorbing Ba (OH) possibly brought in₂) Finally, the prepared CO is removed through a flow dividing device₂Air was delivered to each test apparatus at which time appropriate flow rates were used to remove CO₂Air is delivered to the reaction bottle A (taking the set of test devices as an example), the reaction bottle A contains reaction suspension liquid (such as microorganisms, test substances and the like) required by the test, the magnetic stirring is used for keeping the suspension of the contained substances, the reaction bottle is provided with a capped feeding pipe for feeding, gas is transmitted to an absorption bottle A1 through a pipeline after passing through the reaction bottle, and the absorption bottle A1 absorbs generated carbon dioxide and then performs secondary absorption through an absorption bottle A2 to ensure the sufficiency of absorption.

The biodegradation curve of the test substance can be drawn by only periodically measuring the inorganic carbon content in the absorption bottles A1 and A2 in the test process.

Preferably, the magnetic stirrer rotor arranged in the reaction bottle is made of pure steel.

Preferably, the conveying pipeline comprises a flowmeter for regulating the gas flow required by the test.

Preferably, a fixing knob is arranged between the two-stage filling tanks.

Preferably, a feeding pipe is arranged in the reaction bottle.

Preferably, the CO is₂The absorption bottles are divided into an upper group and a lower group, and can be absorbed for 2 times.

The invention has the technical effects and advantages that:

(1) the invention integrates CO₂The removal unit and the reaction unit have simple system and strong operability, are convenient to assemble in a laboratory and are used for evaluating new chemical substancesBiodegradation test (CO)₂Production method) provides convenience.

(2) CO of the invention₂The removal unit adopts a soda lime filler mode as an absorbent, and compared with the traditional absorption liquid mode, the removal unit is convenient to operate, safe and reliable. The unit is furthermore equipped with CO₂And an efficiency indicating system is removed, so that the effectiveness of the unit is improved.

(3) The reaction bottle is internally provided with the feeding tube, so that the sample can be added without opening the bottle, and the operation is convenient.

(4) The invention is based on CO₂The absorption bottle divide into about two sets of, can carry out 2 times and absorb, can improve and absorb the sufficiency to improve the test accuracy of whole test system.

Drawings

Fig. 1 is a schematic structural view of the whole of the present invention.

In the figure: 1. a reaction unit; 2. CO 2₂A removal unit; 3. CO 2₂An absorption bottle; 4. a reaction bottle; 5. a magnetic stirrer; 6. a stirrer; 7. a capped feed tube; 8. fixing the knob; 9. absorbent cotton; 10. a sieve plate; 11. a fixing device; 12. a flow meter; 13. and (5) a secondary packed column.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The invention provides a test system applied to a biodegradation test, as shown in figure 1, comprising CO₂Removal unit 2, the CO₂The air pump contained in the removing unit 2 conveys air to the secondary filling columns 13, the upper part and the lower part of each secondary filling column 13 are filled with absorbent cotton 9, the secondary filling columns 13 and the absorbent cotton 9 are separated by the sieve plate 10 to ensure that the absorbent cotton is not polluted, the two secondary filling columns 13 are fixed by the fixing device 11, and air guide pipes are arranged above the two secondary filling columns 13The pipes are connected, a fixing knob 8 is arranged between two secondary packing columns 13 to control the fixing degree, and after the reaction of the secondary packing columns 13 is finished, air is conveyed into the secondary packing columns and is filled with Ba (OH)₂The water washing bottle and the water-containing water washing bottle, the water-containing water washing bottle conveys the gas after the reaction into the reaction unit 1 after the flow of the gas is controlled by the flowmeter.

The reaction unit 1 contains CO₂An absorption bottle 3, a reaction bottle 4, a magnetic stirrer 5 arranged below the reaction bottle 4, a stirrer 6 arranged in the reaction bottle 4, a feeding pipe 7 with a cover arranged above the reaction bottle, and CO₂The absorption bottle 3 is divided into an upper group and a lower group, the middle is connected with an air duct, and the upper part is CO₂The absorption bottle 3 can absorb for 2 times, thereby ensuring the absorption sufficiency and improving the test precision.

The working principle of the invention is as follows: when in use, air is pumped into the secondary packing column 13 (absorbent cotton 9 in the packing column can filter out dust and particulate matters), and the porous soda lime can fully absorb CO in the air₂And water) to obtain CO-removed product after absorption₂Air, then passed through a clear glass vial containing barium hydroxide solution, and the CO in air can be characterized by whether a white precipitate is produced₂Removal efficiency (if precipitation occurs, CO is indicated)₂Not removed, the filling replaced, and then rinsed with water (to absorb Ba (OH) that may be carried over)₂) Finally, the prepared CO is removed through a flow dividing device₂The air is fed to the test apparatus, each of the feed lines containing a flow meter 12 for regulating the flow of gas required for the test, in which case the appropriate flow rate is provided for removing CO₂Air is delivered to a reaction bottle 4, reaction suspension liquid (microorganism and test substance) required by the test is contained in the reaction bottle 4, the contained substance is kept suspended by a magnetic stirrer 5 and a stirrer 6, in addition, the reaction bottle 4 is provided with a feeding pipe 7 with a cover, the feeding can be carried out without opening the bottle, and gas is transmitted to CO through a pipeline after passing through the reaction bottle 4₂Absorption bottle 3, Via CO₂After carbon dioxide generated by absorption in the absorption bottle 3 is absorbed, another unused CO passes through the upper part₂The absorption bottle 3 performs secondary absorption to ensure the sufficiency of absorption. The CO is measured regularly in the test process₂The inorganic carbon content in the absorption flask 3,the biodegradation curve of the test substance can be plotted.

In the description of the present invention, unless otherwise expressly specified or limited, the terms "disposed," "mounted," "connected," and "secured" are to be construed broadly, e.g., as meaning fixedly connected, detachably connected, or integral to; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

The standard parts used by the invention can be purchased from the market, and the special-shaped parts can be customized according to the description and the description of the attached drawings.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.