阅读说明：本技术 一种全自动样本玻片处理仪及样本玻片处理方法 (Full-automatic sample slide treatment instrument and sample slide treatment method ) 是由 王涛 李东文 叶莘 曹小强 何杰 陈燕慈 黄萌 林顺迎 于 2021-09-09 设计创作，主要内容包括：本发明涉及生物技术领域,尤其是涉及一种全自动样本玻片处理仪及样本玻片处理方法。本发明提供的全自动样本玻片处理仪,包括：工作台、用于装载样本玻片的玻片架、控制模块和与控制模块电连接的机械臂；工作台包括反应区,反应区内设置有多个反应槽,一个反应槽用于盛放一种反应液；机械臂设置在工作台上,且控制模块控制机械臂将玻片架依次送入每个反应槽中,从而自动完成样本玻片的杂交前预处理和/或杂交后洗脱。使用时,向每个反应槽内均注入反应液,将样本玻片放置在玻片架上,并将玻片架固定在机械臂上,然后通过控制模块控制机械臂将玻片架依次送入每个反应槽中,从而自动完成样本玻片的杂交前预处理和/或杂交后洗脱。(The invention relates to the technical field of biology, in particular to a full-automatic sample slide treatment instrument and a sample slide treatment method. The invention provides a full-automatic sample slide treatment instrument, which comprises: the device comprises a workbench, a slide rack for loading sample slides, a control module and a mechanical arm electrically connected with the control module; the workbench comprises a reaction area, a plurality of reaction tanks are arranged in the reaction area, and one reaction tank is used for containing a reaction liquid; the mechanical arm is arranged on the workbench, and the control module controls the mechanical arm to sequentially send the slide racks into each reaction tank, so that pretreatment before hybridization and/or elution after hybridization of the sample slides are automatically completed. When the device is used, reaction liquid is injected into each reaction tank, a sample slide is placed on the slide rack, the slide rack is fixed on the mechanical arm, and then the mechanical arm is controlled by the control module to sequentially send the slide rack into each reaction tank, so that pretreatment before hybridization and/or elution after hybridization of the sample slide are automatically completed.)

1. A fully automated specimen slide processing apparatus, comprising: the device comprises a workbench (1), a slide rack (2) for loading a sample slide, a control module and a mechanical arm (3) electrically connected with the control module;

the workbench (1) comprises a reaction area (4), a plurality of reaction tanks are arranged in the reaction area (4), and one reaction tank is used for containing a reaction liquid;

the mechanical arm (3) is arranged on the workbench (1), and the control module controls the mechanical arm (3) to sequentially send the slide racks (2) into each reaction tank, so that pretreatment before hybridization and/or elution after hybridization of a sample slide are automatically completed.

2. The fully automatic sample slide processor according to claim 1, wherein said reaction chambers are provided with ten, respectively, a first reaction chamber (5), a second reaction chamber (6), a third reaction chamber (7), a fourth reaction chamber (8), a fifth reaction chamber (9), a sixth reaction chamber (10), a seventh reaction chamber (11), and an eighth reaction chamber (12), a ninth reaction chamber (13), and a tenth reaction chamber (14);

the first reaction tank (5), the second reaction tank (6), the third reaction tank (7), the fourth reaction tank (8), the fifth reaction tank (9), the sixth reaction tank (10), the seventh reaction tank (11) and the eighth reaction tank (12) are used for pre-hybridization pretreatment of a sample slide;

the ninth reaction tank (13) and the tenth reaction tank (14) are used for elution after hybridization of a sample slide.

3. The fully automatic sample slide processor according to claim 2, wherein the first reaction tank (5), the second reaction tank (6), the third reaction tank (7), the fourth reaction tank (8), the fifth reaction tank (9), the sixth reaction tank (10), the seventh reaction tank (11), and the eighth reaction tank (12) are arranged in sequence in a circle, a square, a trapezoid, a straight line or a plurality of rows.

4. The fully automatic sample slide processor according to claim 3, wherein the first reaction tank (5), the second reaction tank (6), the third reaction tank (7), the fourth reaction tank (8), the fifth reaction tank (9), the sixth reaction tank (10), the seventh reaction tank (11), and the eighth reaction tank (12) are sequentially arranged in two rows along a length direction of the work stage (1);

one row is provided with first reaction tank (5), second reaction tank (6), third reaction tank (7) and fourth reaction tank (8), another row is provided with fifth reaction tank (9), sixth reaction tank (10), seventh reaction tank (11) with eighth reaction tank (12), just first reaction tank (5) with eighth reaction tank (12) are corresponding.

5. The fully automated sample slide processing instrument of claim 2, further comprising a temperature control system electrically connected to the control module, the temperature control system for controlling the temperature of the reaction chamber.

6. The fully automated sample slide processor of claim 5, wherein the temperature control system comprises a heating module, a cooling module, and a temperature sensor, the heating module, the cooling module, and the temperature sensor all electrically connected to the control module;

the heating module and the temperature sensor are arranged on the first reaction tank (5), the second reaction tank (6) and the ninth reaction tank (13), and the heating module, the refrigerating module and the temperature sensor are arranged on the tenth reaction tank (14).

7. The fully automated specimen slide processing apparatus according to claim 1, further comprising a liquid discharge system electrically connected to the control module, the plurality of reaction vessels each communicating with the liquid discharge system, the control module controlling the liquid discharge system to discharge the reaction liquid in each of the reaction vessels.

8. The full-automatic sample slide processor according to claim 7, wherein the drainage system comprises a drainage pump, a confluence device, a plurality of drainage solenoid valves (18) and a plurality of drainage pipes which are equal to the number of the reaction tanks and are independent from each other, one reaction tank is communicated with the confluence device through one drainage pipe, each drainage pipe is provided with the drainage solenoid valve (18), and the drainage port of the confluence device is communicated with the drainage pump;

the liquid discharge pump and the liquid discharge electromagnetic valve (18) are electrically connected with the control module.

9. The fully automated specimen slide processor of claim 1, wherein the workstation (1) further comprises a reagent zone (15), a reagent vial (16) being disposed within the reagent zone (15);

the full-automatic sample slide processor also comprises a liquid injection system electrically connected with the control module, one reagent bottle (16) is communicated with a plurality of reaction grooves through the liquid injection system, or the reagent bottle (16) is communicated with the reaction grooves in a one-to-one mode through the liquid injection system, or the plurality of reagent bottles (16) are communicated with one reaction groove through the liquid injection system;

the control module controls the liquid injection system to inject the reaction liquid in the reagent bottle (16) into the reaction tank communicated with the reaction liquid.

10. The fully automated specimen slide processor of claim 9, wherein the reagent bottles (16) are provided in three, and the reaction wells are provided in at least three;

the liquid injection system comprises three mutually independent liquid injection pipes, and the reagent bottles (16) are communicated with the reaction tank one by one through the liquid injection pipes;

every all be provided with infusion pump (20) and notes liquid solenoid valve (17) on the notes liquid pipe, infusion pump (20) with annotate liquid solenoid valve (17) all with control module electricity is connected.

11. The fully automatic sample slide processor according to any one of claims 1 to 10, wherein a holding cavity (30) is formed in the working platform, the working platform (1) is provided with a number of first mounting holes (24) equal to the number of the reaction grooves, the first mounting holes (24) are communicated with the holding cavity (30), and the reaction grooves are one-to-one mounted in the first mounting holes (24).

12. The fully automated specimen slide processor of claim 11, further comprising a waterproof pad (33), the waterproof pad (33) defining a second mounting hole (32) that mates with the first mounting hole (24);

the waterproof pad (33) is installed on the workbench, and the first installation hole (24) corresponds to the second installation hole (32).

13. The fully automated sample slide processing instrument of claim 11, further comprising a heat dissipation system (27) electrically connected to the control module, the heat dissipation system (27) configured to dissipate heat from the receiving chamber (30).

14. The fully automated sample slide processing apparatus according to any one of claims 1 to 10, further comprising a protective housing (26), wherein the protective housing (26) is covered on the working platform (1), and an instrument door (25) is arranged on the protective housing (26).

15. A sample slide processing method characterized by performing the following steps using the fully automatic sample slide processing apparatus according to any one of claims 1 to 14:

pre-hybridization pretreatment and/or post-hybridization elution:

reaction liquid is respectively injected into the reaction tanks;

placing the sample slide on the slide rack (2) and mounting the slide rack (2) on the mechanical arm (3);

the control module controls the mechanical arm (3) to sequentially send the slide racks (2) into each reaction tank, so that pretreatment before hybridization and/or elution after hybridization of the sample slide are completed.

Technical Field

The invention relates to the technical field of biology, in particular to a full-automatic sample slide treatment instrument and a sample slide treatment method.

Background

The fluorescence in situ hybridization technique is to label a nucleic acid probe with a reporter molecule (such as a fluorescent dye, biotin, digoxigenin, etc.), then hybridize the probe with a target DNA, and if the two are homologous complementary, a hybrid of the target DNA and the nucleic acid probe can be formed. The reporter molecule can then be used to perform qualitative, quantitative or relative localization analysis of the target DNA under a fluorescence microscope.

In the prior art, the main procedures of the fluorescence in situ hybridization technology are as follows: preparing a sample, pretreating, dripping a probe, hybridizing, eluting after hybridization and counterstaining, wherein the hybridization is carried out by adopting a temperature control device, and the sample is only put into the device for operation without other operations; the preparation of the sample, the probe dripping and the counterstaining are carried out by manual treatment; and the pretreatment and the elution after hybridization are operated by adopting a device capable of heating and preserving heat, the intermediate process has multiple steps, the sample needs to be repeatedly soaked and cleaned in different reagent environments, and the sample can be sent to a microscope for scanning observation and analysis after all the procedures are completed in sequence.

In the process of elution after pretreatment or hybridization, the sample is required to be soaked in at least two different test solution environments, the device has no automatic switching function, the sample can be switched one by only depending on the manual work, the process can bring the individual error of manual operation, the specific error comprises inconsistent speed of manually taking and placing the sample, inconsistent order of taking and placing, inconsistent time of stay between taking and placing and the like, the individual error of the manual operation can influence the hybridization experiment result to a certain extent, the hybridization experiment result is inaccurate, human resources are occupied greatly, the reaction time of each process in the whole pretreatment and elution process after hybridization is short, the whole-process tracking operation of people is required, and the labor is seriously wasted.

Disclosure of Invention

The invention aims to provide a full-automatic sample slide treatment instrument and a sample slide treatment method, which are used for releasing the labor force of personnel, improving the experiment repeatability and reducing the difference between batches.

The invention provides a full-automatic sample slide treatment instrument, which comprises: the device comprises a workbench, a slide rack for loading sample slides, a control module and a mechanical arm electrically connected with the control module;

the workbench comprises a reaction area, a plurality of reaction tanks are arranged in the reaction area, and one reaction tank is used for containing a reaction liquid;

the mechanical arm is arranged on the workbench, and the control module controls the mechanical arm to sequentially send the slide racks into each reaction tank, so that pretreatment before hybridization and/or elution after hybridization of the sample slide are automatically completed.

As a further technical scheme, the reaction tanks are ten and are respectively a first reaction tank, a second reaction tank, a third reaction tank, a fourth reaction tank, a fifth reaction tank, a sixth reaction tank, a seventh reaction tank, an eighth reaction tank, a ninth reaction tank and a tenth reaction tank;

the first reaction tank, the second reaction tank, the third reaction tank, the fourth reaction tank, the fifth reaction tank, the sixth reaction tank, the seventh reaction tank and the eighth reaction tank are used for pretreatment before hybridization of a sample slide;

the ninth reaction tank and the tenth reaction tank are used for elution after hybridization of a sample slide.

As a further technical solution, the first reaction tank, the second reaction tank, the third reaction tank, the fourth reaction tank, the fifth reaction tank, the sixth reaction tank, the seventh reaction tank and the eighth reaction tank are sequentially arranged in a circle, a square, a trapezoid, a straight line or a plurality of rows.

As a further technical solution, the first reaction tank, the second reaction tank, the third reaction tank, the fourth reaction tank, the fifth reaction tank, the sixth reaction tank, the seventh reaction tank and the eighth reaction tank are sequentially arranged in two rows along the length direction of the worktable;

one row is provided with first reaction tank second reaction tank third reaction tank with fourth reaction tank, another row is provided with fifth reaction tank sixth reaction tank seventh reaction tank with the eighth reaction tank, just first reaction tank with the eighth reaction tank corresponds.

As a further technical solution, the fully automatic sample slide processing instrument further comprises a temperature control system electrically connected with the control module, and the temperature control system is used for controlling the temperature of the reaction tank.

As a further technical scheme, the temperature control system comprises a heating module, a refrigerating module and a temperature sensor, wherein the heating module, the refrigerating module and the temperature sensor are all electrically connected with the control module;

the heating module and the temperature sensor are arranged on the first reaction tank, the second reaction tank and the ninth reaction tank, and the heating module, the refrigerating module and the temperature sensor are arranged on the tenth reaction tank.

As a further technical scheme, the full-automatic sample slide treatment instrument further comprises a liquid discharge system electrically connected with the control module, the reaction grooves are all communicated with the liquid discharge system, and the control module controls the liquid discharge system to discharge the reaction liquid in each reaction groove.

As a further technical scheme, the liquid discharge system comprises a liquid discharge pump, a confluence device, liquid discharge electromagnetic valves and liquid discharge pipes which are equal in number to the reaction tanks and are independent of each other, one reaction tank is communicated with the confluence device through one liquid discharge pipe, each liquid discharge pipe is provided with the liquid discharge electromagnetic valve, and a liquid discharge port of the confluence device is communicated with the liquid discharge pump;

the liquid discharge pump and the liquid discharge electromagnetic valve are electrically connected with the control module.

As a further technical scheme, the workbench further comprises a reagent area, wherein a reagent bottle is arranged in the reagent area;

the full-automatic sample slide treatment instrument also comprises a liquid injection system electrically connected with the control module, one reagent bottle is communicated with the reaction grooves through the liquid injection system, or the reagent bottle is communicated with the reaction grooves in a one-to-one mode through the liquid injection system, or the reagent bottles are communicated with one reaction groove through the liquid injection system;

the control module controls the liquid injection system to inject the reaction liquid in the reagent bottle into the reaction tank communicated with the reagent bottle.

As a further technical scheme, the number of the reagent bottles is three, and the number of the reaction tanks is at least three;

the liquid injection system comprises three mutually independent liquid injection pipes, and the reagent bottles are communicated with the reaction tank one to one through the liquid injection pipes;

every all be provided with the priming pump on the notes liquid pipe and annotate the liquid solenoid valve, the priming pump with annotate the liquid solenoid valve all with control module electricity is connected.

As a further technical scheme, an accommodating cavity is formed in the workbench, first mounting holes with the same number as the reaction grooves are formed in the workbench and communicated with the accommodating cavity, and the reaction grooves are mounted in the first mounting holes in a one-to-one mode.

As a further technical scheme, the waterproof structure also comprises a waterproof pad, wherein the waterproof pad is provided with a second mounting hole matched with the first mounting hole;

the waterproof pad is installed on the workbench, and the first installation hole corresponds to the second installation hole.

As a further technical scheme, the full-automatic sample slide processor further comprises a heat dissipation system electrically connected with the control module, and the heat dissipation system is used for dissipating heat of the accommodating cavity.

As a further technical scheme, the full-automatic sample slide treatment instrument further comprises a protective shell, wherein the protective shell is covered on the workbench, and an instrument door is arranged on the protective shell.

The invention provides a sample slide processing method, which utilizes the full-automatic sample slide processor to carry out the following steps:

pre-hybridization pretreatment and/or post-hybridization elution:

reaction liquid is respectively injected into the reaction tanks;

placing the sample slide on a slide rack and mounting the slide rack on a robotic arm;

the control module controls the mechanical arm to sequentially send the slide racks into each reaction tank, so that pretreatment before hybridization and/or elution after hybridization of the sample slides are completed.

Compared with the prior art, the full-automatic sample slide treatment instrument and the sample slide treatment method provided by the invention have the technical advantages that:

the invention provides a full-automatic sample slide treatment instrument, which comprises: the device comprises a workbench, a slide rack for loading sample slides, a control module and a mechanical arm electrically connected with the control module; the workbench comprises a reaction area, a plurality of reaction tanks are arranged in the reaction area, and one reaction tank is used for containing a reaction liquid; the mechanical arm is arranged on the workbench, and the control module controls the mechanical arm to sequentially send the slide racks into each reaction tank, so that pretreatment before hybridization and/or elution after hybridization of the sample slides are automatically completed.

When the device is used, reaction liquid is injected into each reaction tank, a sample slide is placed on a slide rack, the slide rack is fixed on a mechanical arm, and then the mechanical arm is controlled by a control module to sequentially send the slide rack into each reaction tank, so that pretreatment before hybridization and/or elution after hybridization of the sample slide are automatically completed; when the reaction liquid in the reaction tank is used for the pretreatment before hybridization of the sample slide, namely, the pretreatment before hybridization of the sample slide is automatically completed, when the reaction liquid in the reaction tank is used for the elution after hybridization of the sample slide, namely, the elution after hybridization of the sample slide is automatically completed, and when the reaction liquid in the reaction tank is used for the pretreatment before hybridization and the elution after hybridization of the sample slide, namely, the pretreatment before hybridization and the elution after hybridization of the sample slide are automatically completed. The mechanical arm is controlled by the control module to sequentially send the slide racks into each reaction tank, so that errors caused by manual operation are reduced, accuracy of hybridization experiment results is improved, labor force of personnel is liberated, experiment repeatability is improved, and batch difference is reduced.

The sample slide processing method provided by the invention utilizes the full-automatic sample slide processing instrument, so that the technical advantages and effects achieved by the sample slide processing instrument comprise the technical advantages and effects achieved by the sample slide processing instrument, and are not described in detail herein.

Additional features and advantages of the invention will be set forth in the detailed description which follows.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.

FIG. 1 is an exploded view of a fully automated specimen slide processing apparatus provided by an embodiment of the present invention;

FIG. 2 is a front view of an instrument door provided in accordance with an embodiment of the present invention, shown open;

FIG. 3 is a front view of an instrument door according to an embodiment of the present invention;

FIG. 4 is a side cross-sectional view of a fully automated sample slide processor provided in accordance with an embodiment of the present invention;

FIG. 5 is a side rear view of a fully automated specimen slide processor provided in accordance with an embodiment of the present invention;

FIG. 6 is an internal view of a table provided by an embodiment of the present invention;

FIG. 7 is a top view of a table provided in accordance with an embodiment of the present invention;

FIG. 8 is an exploded view of a normal temperature reaction tank according to an embodiment of the present invention;

FIG. 9 is an exploded view of a dual-valve normal temperature reaction tank according to an embodiment of the present invention;

fig. 10 is a schematic structural view of a waterproof pad according to an embodiment of the present invention.

Icon: 1-a workbench; 2-a slide rack; 3, a mechanical arm; 4-a reaction zone; 5-a first reaction tank; 6-a second reaction tank; 7-a third reaction tank; 8-a fourth reaction tank; 9-a fifth reaction tank; 10-a sixth reaction tank; 11-a seventh reaction tank; 12-an eighth reaction tank; 13-ninth reaction tank; 14-tenth reaction tank; 15-reagent zone; 16-a reagent bottle; 17-liquid injection electromagnetic valve; 18-liquid outlet electromagnetic valve; 19-a base; 20-a liquid injection pump; 21-industrial control computer; 22-a pretreatment region; 23-post-hybridization elution zone; 24-a first mounting hole; 25-instrument gate; 26-a protective housing; 27-a heat dissipation system; 28-a trough body; 29-a power module; 30-a containment chamber; 31-hanging hook; 32-a second mounting hole; 33-waterproof cushion.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

In addition, technical solutions between various embodiments may be combined with each other, but must be realized by a person skilled in the art, and when the technical solutions are contradictory or cannot be realized, such a combination should not be considered to exist, and is not within the protection scope of the present invention.

The present invention will be described in further detail below with reference to specific embodiments and with reference to the attached drawings.

The specific structure is shown in fig. 1 to 10.

The present embodiment provides a fully automatic sample slide processing instrument, comprising: workstation 1, a slide frame 2 for loading sample slide, control module and the arm 3 of being connected with the control module electricity, be provided with the couple 31 that is used for articulating slide frame 2 on the arm 3, workstation 1 includes reaction zone 4, be provided with a plurality of reaction tanks in the reaction zone 4, every reaction tank is used for holding a reaction liquid, control module control arm 3 sends into slide frame 2 in proper order in every reaction tank, thereby wash after preliminary treatment and/or the hybridization before the automatic hybridization of accomplishing sample slide. The worktable 1 comprises a table top and a base 19, the table top comprises a reaction area 4, the mechanical arm 3 is arranged on the table top, and the full-automatic sample slide treatment instrument also comprises a power module 29 which is electrically connected with the control module and a power module 29 which provides electric energy for the control module.

It should be noted that, after the reaction tanks are filled with the reaction solution, one reaction tank corresponds to one process in the pretreatment before hybridization or the elution after hybridization of the sample slide, and since the pretreatment before hybridization or the elution after hybridization of the sample slide needs to be soaked in the environment of a plurality of reaction solutions, the plurality of reaction solutions needed in the pretreatment before hybridization or the elution after hybridization of the sample slide are respectively filled into different reaction tanks, so that only one reaction solution exists in one reaction tank.

When the device is used, a reaction liquid is injected into each reaction tank, a sample slide is placed on the slide rack 2, the slide rack 2 is fixed on the hook 31 of the mechanical arm 3, and then the mechanical arm 3 is controlled by the control module to sequentially send the slide racks 2 into each reaction tank, so that pretreatment before hybridization and/or elution after hybridization of the sample slide are automatically completed; when the reaction liquid in the reaction tank is used for the pretreatment before hybridization of the sample slide, namely, the pretreatment before hybridization of the sample slide is automatically completed, when the reaction liquid in the reaction tank is used for the elution after hybridization of the sample slide, namely, the elution after hybridization of the sample slide is automatically completed, and when the reaction liquid in the reaction tank is used for the pretreatment before hybridization and the elution after hybridization of the sample slide, namely, the pretreatment before hybridization and the elution after hybridization of the sample slide are automatically completed. In sending into every reaction tank in proper order slide frame 2 through control module control arm 3, on the one hand, because control module can accurate control arm 3's operating duration and the time that gets into in the reaction tank, consequently can reduce the error that manual operation caused, improve the accuracy of hybridization experimental result, liberation personnel's labour simultaneously, on the other hand improves experiment repeatability, reduces batch-to-batch difference.

In an optional technical solution of this embodiment, nine reaction tanks are provided, which are respectively a first reaction tank 5, a second reaction tank 6, a third reaction tank 7, a fourth reaction tank 8, a sixth reaction tank 10, a seventh reaction tank 11, an eighth reaction tank 12, a ninth reaction tank 13, and a tenth reaction tank 14.

The reaction zone 4 comprises a pre-hybridization pretreatment zone 22 and a post-hybridization elution zone 23, and a first reaction tank 5, a second reaction tank 6, a third reaction tank 7, a fourth reaction tank 8, a sixth reaction tank 10, a seventh reaction tank 11 and an eighth reaction tank 12 which are used for pre-hybridization treatment of the sample slide are arranged in the pre-hybridization pretreatment zone 22; the ninth reaction tank 13 and the tenth reaction tank 14 for elution after hybridization of the slide specimen are provided in the post-hybridization elution zone 23.

In this embodiment, the pretreatment before hybridization of the sample slide requires seven kinds of reaction solutions to soak, and therefore, the first reaction tank 5, the second reaction tank 6, the third reaction tank 7, the fourth reaction tank 8, the sixth reaction tank 10, the seventh reaction tank 11, and the eighth reaction tank 12 for respectively accommodating the seven kinds of reaction solutions are correspondingly disposed in the pretreatment area 22 before hybridization, wherein, since one of the seven kinds of reaction solutions needs to soak twice, the robot arm 3 needs to send the slide rack 2 into the reaction tank containing the reaction solution twice.

In this embodiment, since the elution of the sample slide after hybridization requires the immersion of two reaction solutions, a ninth reaction tank 13 and a tenth reaction tank 14 for accommodating the two reaction solutions respectively are provided in the elution zone 23 after hybridization.

According to the arrangement, the full-automatic sample slide treatment instrument can automatically complete pretreatment before hybridization and elution after hybridization of a sample slide, on one hand, errors caused by manual operation in two procedures in the fluorescence in situ hybridization technology are reduced, accuracy of hybridization experiment results is improved, labor force of workers in the two procedures in the fluorescence in situ hybridization technology is liberated, on the other hand, experiment repeatability in the two procedures in the fluorescence in situ hybridization technology is improved, and batch difference is reduced.

In an alternative embodiment of this embodiment, the plurality of reaction chambers further includes a fifth reaction chamber 9, the fifth reaction chamber 9 is disposed in the pre-hybridization pretreatment region 22 for pre-hybridization pretreatment of the sample slide, because one of seven reaction solutions required by pretreatment before hybridization of the sample slide needs to be soaked twice, therefore, the fifth reaction tank 9 is added, so that the reaction liquid which needs to be soaked twice can be contained in the two reaction tanks, thus, the mechanical arm 3 only needs to send the slide racks 2 into the first reaction tank 5, the second reaction tank 6, the third reaction tank 7, the fourth reaction tank 8, the fifth reaction tank 9, the sixth reaction tank 10, the seventh reaction tank 11 and the eighth reaction tank 12 in sequence, the pretreatment before hybridization of the sample slide can be automatically completed, so that the running track of the mechanical arm 3 is simple, the mechanical arm does not need to enter a reaction tank twice, and the cross contamination is prevented.

In an optional technical scheme of this embodiment, the first reaction tank 5, the second reaction tank 6, the third reaction tank 7, the fourth reaction tank 8, the fifth reaction tank 9, the sixth reaction tank 10, the seventh reaction tank 11, and the eighth reaction tank 12 are sequentially arranged in a circle, a square, a trapezoid, a straight line or a plurality of rows.

Specifically, a first reaction tank 5, a second reaction tank 6, a third reaction tank 7, a fourth reaction tank 8, a fifth reaction tank 9, a sixth reaction tank 10, a seventh reaction tank 11 and an eighth reaction tank 12 are all arranged in the middle of the workbench 1, and are sequentially arranged in two rows along the length direction of the workbench 1; one row is provided with a first reaction tank 5, a second reaction tank 6, a third reaction tank 7 and a fourth reaction tank 8, the other row is provided with a fifth reaction tank 9, a sixth reaction tank 10, a seventh reaction tank 11 and an eighth reaction tank 12, and the first reaction tank 5 corresponds to the eighth reaction tank 12. The ninth reaction tank 13 and the tenth reaction tank 14 are also provided along the longitudinal direction of the table 1, and the tenth reaction tank 14 is located close to the outside. The length direction of the workbench 1 means that a person stands in front of the workbench 1, and the left and right directions of the person are the length direction of the workbench 1 at the moment.

So set up, compact structure, improve the space utilization of workstation 1, it is relatively less to make workstation 1 can make, simultaneously first reaction tank 5, second reaction tank 6, third reaction tank 7, fourth reaction tank 8, fifth reaction tank 9, sixth reaction tank 10, seventh reaction tank 11 and eighth reaction tank 12 are arranged in order, when making arm 3 drive slide frame 2 motion, can remove in order, make the motion route shortest, reduce the time of removing the extravagant time of in-process, improve the work efficiency of full-automatic sample slide treatment appearance, can soak in getting into next reaction tank sooner, improve the reaction effect of sample slide, the condition of crossing has also been avoided the arm operation route to appear and lead to the reaction solution drippage in the different reaction tanks to leak into other reaction tanks and cause the pollution, and influence hybridization quality.

The present embodiment is not limited thereto, and the first reaction tank 5, the second reaction tank 6, the third reaction tank 7, the fourth reaction tank 8, the fifth reaction tank 9, the sixth reaction tank 10, the seventh reaction tank 11, and the eighth reaction tank 12 may be arranged in any form as long as the requirements are satisfied. It should be noted that, when the pre-treatment region 22 before hybridization is provided with the first reaction tank 5, the second reaction tank 6, the third reaction tank 7, the fourth reaction tank 8, the sixth reaction tank 10, the seventh reaction tank 11 and the eighth reaction tank 12, the pre-treatment region may be circular, square, trapezoidal, linear, multi-row or irregular, or may be provided in two rows as long as the requirements are met.

In an optional technical solution of this embodiment, the fully automatic sample slide processing apparatus further includes a temperature control system electrically connected to the control module, and the temperature control system is configured to control a temperature of the reaction tank. In the required reaction liquid of preliminary treatment before the sample slide hybridization or elution after the hybridization, some reaction liquid can have better reaction with the sample slide at normal atmospheric temperature, still some reaction liquid reaction efficiency can improve or the dissolving rate of reaction liquid is fast under the environment that is different from the normal atmospheric temperature, and the setting of temperature control system makes control module can pass through temperature control system, and then the temperature of control reaction tank, improves the efficiency and the effect of preliminary treatment before the hybridization or elution after the hybridization like.

In an optional technical scheme of this embodiment, the temperature control system includes a heating module, a refrigeration module and a temperature sensor, and the heating module, the refrigeration module and the temperature sensor are all electrically connected with the control module; heating modules and temperature sensors are arranged on the first reaction tank 5, the second reaction tank 6 and the ninth reaction tank 13 to form a heating reaction tank, and a heating module, a refrigerating module and a temperature sensor are arranged on the tenth reaction tank 14 to form a heating and refrigerating reaction tank.

In this embodiment, when the sample slide is pretreated before hybridization and eluted after hybridization, the first reaction tank 5, the second reaction tank 6 and the ninth reaction tank 13 are provided with a heating module and a temperature sensor, the control module controls the heating module to heat the first reaction tank 5, the second reaction tank 6 and the ninth reaction tank 13, and the feedback control module is performed through the temperature sensor until the temperature is heated to a preset temperature, so that the reaction effect of the reaction solution in the first reaction tank 5, the second reaction tank 6 and the ninth reaction tank 13 is better. The control module controls the heating module to set the heating ranges of the first reaction tank 5, the second reaction tank 6 and the ninth reaction tank 13 at any temperature between room temperature and 80 ℃, and can heat the reaction tanks to the set temperature within 15 minutes and stably, the deviation value is +/-1 ℃, and the uniformity is less than or equal to 2 ℃.

The tenth reaction tank 14 is provided with a heating module and a refrigerating module, the control module controls the heating module or the refrigerating module to heat or refrigerate the tenth reaction tank 14, and the feedback control module is carried out through the temperature sensor until the tenth reaction tank is heated to a preset temperature or refrigerated to the preset temperature, so that the reaction effect of the reaction liquid in the tenth reaction tank 14 and the sample slide is improved. The control module controls the heating module to set the heating range of the tenth reaction tank 14 to be selectable from room temperature to 40 ℃, the set temperature can be reached and stabilized within 10 minutes, the deviation value is +/-1 ℃, and the uniformity is less than or equal to 2 ℃; the control module controls the refrigeration module to set the refrigeration range of the tenth reaction tank 14 between 0 ℃ and 40 ℃, and the refrigeration is started from 40 ℃ to reach below 25 ℃ within 10 minutes.

In the optional technical scheme of this embodiment, full-automatic sample slide treatment instrument still includes the flowing back system of being connected with the control module electricity, has all seted up the leakage fluid dram on every reaction tank, and the leakage fluid dram on every reaction tank all communicates with the flowing back system, and the control module control flowing back system is discharged the reaction liquid in every reaction tank. So, realize the automatic flowing back of reaction tank, practice thrift the manpower, raise the efficiency, degree of automation is high.

In the optional technical scheme of this embodiment, the liquid discharge system includes a liquid discharge pump, a confluence device, liquid discharge electromagnetic valves 18 and liquid discharge pipes which are equal in number to the reaction tanks and are independent of each other, one reaction tank is communicated with the confluence device through one liquid discharge pipe, each liquid discharge pipe is provided with a liquid discharge electromagnetic valve 18, and a liquid discharge port of the confluence device is communicated with the liquid discharge pump; the liquid discharge pump and the liquid discharge electromagnetic valve 18 are electrically connected with the control module.

Specifically, the liquid discharge system comprises ten liquid discharge pipes which are mutually independent, and a first reaction tank 5, a second reaction tank 6, a third reaction tank 7, a fourth reaction tank 8, a fifth reaction tank 9, a sixth reaction tank 10, a seventh reaction tank 11, an eighth reaction tank 12, a ninth reaction tank 13, a tenth reaction tank 14 are communicated with the ten liquid discharge pipes in a one-to-one manner; ten drain pipes all communicate with the inlet of collection flow device, play liquid solenoid valve 18 is provided with ten, be provided with one on every drain pipe and go out liquid solenoid valve 18, collection flow device's leakage fluid dram and flowing back pump intercommunication, control module can control first reaction tank 5, second reaction tank 6, third reaction tank 7, fourth reaction tank 8, fifth reaction tank 9, sixth reaction tank 10, seventh reaction tank 11, eighth reaction tank 12, ninth reaction tank 13 and tenth reaction tank 14 and carry out the flowing back through controlling the opening and closing of going out liquid solenoid valve 18 and flowing back pump.

The embodiment is not limited to this, it may also be that each drain pipe is provided with a drain pump and a drain electromagnetic valve 18, the control module controls the simultaneous opening and closing of the drain electromagnetic valve 18 and the drain pump on each drain pipe to respectively control the first reaction tank 5, the second reaction tank 6, the third reaction tank 7, the fourth reaction tank 8, the fifth reaction tank 9, the sixth reaction tank 10, the seventh reaction tank 11, the eighth reaction tank 12, the ninth reaction tank 13 and the tenth reaction tank 14 to drain liquid, the control is independent of each other, and a specific liquid drainage sequence and time can be selected according to requirements.

Among the optional technical scheme of this embodiment, workstation 1 still includes reagent district 15, is provided with reagent bottle 16 in the reagent district 15, and reagent bottle 16 can be provided with one or more, has held a reaction liquid in every reagent bottle 16, and full-automatic sample slide treatment appearance still includes the notes liquid system of being connected with the control module electricity, and a reagent bottle 16 communicates with a plurality of reaction tanks through annotating the liquid system, or reagent bottle 16 communicates one to one through annotating liquid system and reaction tank, or a plurality of reagent bottles 16 pass through annotate liquid system and a reaction tank intercommunication, control module control annotates the reaction liquid injection in reagent bottle 16 into the reaction tank rather than communicating with the control module control liquid system, realizes the automation of reaction tank and annotates liquid, and annotates the liquid volume controllable, practices thrift the manpower, raises the efficiency, and degree of automation is high.

In this embodiment, preferably, the number of the reagent bottles 16 may be the same as the number of the reaction tanks, and the reagent bottles are communicated one by one through the liquid injection system, so that all the reaction tanks realize the automatic liquid injection function; the number of the reagent bottles 16 can be less than that of the reaction tanks, and the reagent bottles are communicated one by one through a liquid injection system, so that the reaction tanks communicated one by one with the reagent bottles 16 all realize an automatic liquid injection function.

Specifically, the reagent bottles 16 are provided with three reagent bottles, namely a first reagent bottle, a second reagent bottle and a third reagent bottle, the liquid injection system comprises three liquid injection pipes which are mutually independent, liquid injection ports are respectively formed in the sixth reaction tank 10, the seventh reaction tank 11 and the eighth reaction tank 12, and the liquid injection ports of the first reagent bottle and the sixth reaction tank 10, the liquid injection ports of the second reagent bottle and the seventh reaction tank 11 and the liquid injection ports of the third reagent bottle and the eighth reaction tank 12 are communicated in a one-to-one mode through the three liquid injection pipes; every annotate and all be provided with infusion pump 20 and annotate liquid solenoid valve 17 on the liquid pipe, annotate liquid pump 20 and annotate liquid solenoid valve 17 and all be connected with control module electricity, control module opens and close through the liquid solenoid valve 17 of annotating on every notes liquid pipe and annotate liquid pump 20 the while, can control first reagent bottle to annotate liquid in sixth reaction tank 10, second reagent bottle annotates liquid in seventh reaction tank 11 and third reagent bottle annotates liquid in eighth reaction tank 12 respectively. The liquid injection device is mutually independently controlled, and specific liquid injection sequence and time can be selected according to requirements.

Wherein the third reaction tank 7, the fourth reaction tank 8 and the fifth reaction tank 9 are all normal temperature reaction tanks; the liquid injection electromagnetic valve 17 and the liquid outlet electromagnetic valve 18 are arranged on the sixth reaction tank 10, the seventh reaction tank 11 and the eighth reaction tank 12, so that the reaction tanks are double-valve normal-temperature reaction tanks.

In the optional technical scheme of this embodiment, be formed with in the workstation and hold chamber 30, set up the first mounting hole 24 the same with the reaction tank quantity on the workstation 1, and first mounting hole 24 with hold chamber 30 intercommunication, the one-to-one installation of reaction tank is in first mounting hole 24.

Specifically, the workbench 1 is provided with first mounting holes 24 with the same number as the reaction tanks, namely ten first mounting holes 24, the pre-hybridization pretreatment region 22 is internally provided with eight first mounting holes 24 which are arranged in two rows, four in each row and aligned with each other, the first reaction tank 5, the second reaction tank 6, the third reaction tank 7, the fourth reaction tank 8, the fifth reaction tank 9, the sixth reaction tank 10, the seventh reaction tank 11 and the eighth reaction tank 12 are correspondingly arranged in the eight first mounting holes 24 in the pre-hybridization pretreatment region 22 one by one, the post-hybridization elution region 23 is internally provided with two first mounting holes 24, the ninth reaction tank 13 and the tenth reaction tank 14 are correspondingly arranged in the two first mounting holes 24 in the post-hybridization elution region 23 one by one, the reaction tanks are arranged in the first mounting holes 24, so that the reaction tanks are more stable relative to the workbench 1, and are embedded in the first mounting holes 24 and positioned below the table top of the workbench 1, the whole reaction tank is not exposed and is beautiful.

This embodiment is preferred, the reaction tank includes cell body 28 and turn-ups, wherein first reaction tank 5, second reaction tank 6, third reaction tank 7, fourth reaction tank 8, fifth reaction tank 9, sixth reaction tank 10, seventh reaction tank 11 and eighth reaction tank 12 all are provided with the little turn-ups that is used for the overlap joint on workstation 1, ninth reaction tank 13 and tenth reaction tank 14 all are provided with the big turn-ups that is used for the overlap joint on workstation 1, the setting up of little turn-ups and big turn-ups makes the width of two rows of reaction tanks in the preliminary treatment zone 22 before the hybridization equal with the width of reaction tank in the elution zone 23 after the hybridization, overall structure is pleasing to the eye.

In an optional technical scheme of this embodiment, the waterproof structure further includes a waterproof pad 33, and the waterproof pad 33 is provided with a second mounting hole 32 matched with the first mounting hole 24; the waterproof pad 33 is installed on the work table, and the first installation hole 24 corresponds to the second installation hole 32.

In this embodiment, the table top of the workbench 1 is provided with the same number of waterproof pads 33 as the number of the reaction tanks, one waterproof pad 33 is provided with one second mounting hole 32, one waterproof pad 33 is mounted at each first mounting hole 24, and each first mounting hole 24 corresponds to the second mounting hole 32 on the corresponding waterproof pad 33; or a waterproof pad 33 is arranged on the table top of the workbench 1, the waterproof pad 33 is provided with second mounting holes 32 with the number equal to that of the reaction tanks, and the first mounting holes 24 correspond to the second mounting holes 32 one by one.

In the optional technical scheme of this embodiment, full-automatic sample slide treatment instrument still includes the cooling system 27 that is connected with the control module electricity, control module control cooling system 27 dispels the heat to holding chamber 30, the part of the first mounting hole 24 of reaction tank embedding, heating module and refrigeration module all are located and hold chamber 30, cooling system 27 is located and holds chamber 30, the part that holds chamber 30 to the reaction tank embedding, heating module, refrigeration module, hold power module 29 and the control module in the chamber 30 and dispel the heat, prevent that the reaction tank that the temperature is high from passing through the space and transferring heat and influencing the low reaction tank of temperature, heating module and refrigeration module self are overheated and hold power module 29 and the control module in the chamber 30 high temperature.

In an optional technical solution of this embodiment, the full-automatic sample slide processing apparatus further includes a protective casing 26, the protective casing 26 covers the workbench 1, and an instrument door 25 is disposed at a front end of the protective casing 26. The upper end of instrument door 25 articulates at the roof front end of protecting crust, opens full-automatic sample slide through upwards overturning instrument door 25 and handles the appearance, makes reaction zone 4, reagent district 15 and arm 3 expose, makes things convenient for the experiment operation, opens full-automatic sample slide through upwards overturning instrument door 25 and handles the appearance, covers the mesa of whole workstation 1, plays the guard action to the facility on the workstation 1.

In this embodiment, the front end surface of the workbench 1 is provided with an industrial personal computer 21, the industrial personal computer 21 includes a control module, and a specific control flow of the control module can be set through a touch screen of the industrial personal computer 21.

When the experimental reaction is started, namely the full-automatic sample slide processor automatically carries out pretreatment before hybridization or elution after hybridization, the instrument door 25 is closed, and the external environment interference is prevented. In this embodiment, instrument door 25 is glass door or ya keli plastics door, and is preferred, and instrument door 25 can see inside behavior for dark transparent yakeli door can see through instrument door 25, can prevent again that external light from causing the influence to internal reaction.

In an optional technical scheme of the embodiment, the full-automatic sample slide treatment instrument further comprises an alarm system, the alarm system is electrically connected with the control module, when the reaction tank is heated to a set temperature and reaction liquid needs to be added, the alarm system gives an alarm to prompt a worker to add the reaction liquid, when the control module controls the mechanical arm 3 to complete a pretreatment before hybridization or an elution process after hybridization of a sample slide, the alarm system gives an alarm to prompt the worker to complete a reaction; when the control module controls the mechanical arm 3 to carry out pretreatment before hybridization or elution after hybridization, a fault occurs or other conditions that the continuous work cannot be carried out, the alarm system gives an alarm to prompt a worker that the fault occurs.

The sample slide processing method provided by the embodiment utilizes the fully automatic sample slide processing instrument to perform the following steps:

pre-hybridization pretreatment and/or post-hybridization elution:

reaction liquid is respectively injected into the reaction tanks;

placing a sample slide on the slide rack 2 and mounting the slide rack 2 onto the robotic arm 3;

the control module controls the mechanical arm 3 to sequentially send the slide racks 2 into each reaction tank, so that pretreatment before hybridization and/or elution after hybridization of the sample slide are completed.

Specifically, pretreatment before hybridization:

the first reaction tank 5 injects the first reaction liquid, the second reaction tank 6 injects the second reaction liquid, the third reaction tank 7 injects the third reaction liquid, the fourth reaction tank 8 injects the fourth reaction liquid and the fifth reaction tank 9 injects the fifth reaction liquid;

injecting a sixth reaction liquid into the first reagent bottle, injecting a seventh reaction liquid into the second reagent bottle, and injecting an eighth reaction liquid into the third reagent bottle;

the control module controls the heating module on the first reaction tank 5 to heat the first reaction tank 5 to a stable temperature to be reacted and keep the temperature, and the heating module on the second reaction tank 6 heats the second reaction tank 6 to a stable temperature to be reacted and keep the temperature;

placing a sample slide on the slide rack 2 and mounting the slide rack 2 onto the robotic arm 3;

the control module controls the liquid injection pump 20 and the liquid injection electromagnetic valve 17 to inject the sixth reaction liquid in the first reagent bottle into the sixth reaction tank 10 through the liquid injection pipe, inject the seventh reaction liquid in the second reagent bottle into the seventh reaction tank 11 through the liquid injection pipe, and inject the eighth reaction liquid in the third reagent bottle into the eighth reaction tank 12 through the liquid injection pipe;

the control module controls the mechanical arm 3 to sequentially send the slide frame 2 into a first reaction tank 5, a second reaction tank 6, a third reaction tank 7, a fourth reaction tank 8, a fifth reaction tank 9, a sixth reaction tank 10, a seventh reaction tank 11 and an eighth reaction tank 12, so that pretreatment before hybridization of a sample slide is completed;

the control module controls the liquid discharge system to discharge the reacted reaction liquid from the first reaction tank 5, the second reaction tank 6, the third reaction tank 7, the fourth reaction tank 8, the fifth reaction tank 9, the sixth reaction tank 10, the seventh reaction tank 11 and the eighth reaction tank 12, namely the control module controls the liquid discharge pump and the liquid discharge electromagnetic valve 18 to discharge the reaction liquid in the first reaction tank 5, the second reaction tank 6, the third reaction tank 7, the fourth reaction tank 8, the fifth reaction tank 9, the sixth reaction tank 10, the seventh reaction tank 11 and the eighth reaction tank 12, and simultaneously manually sends the sample slide to the probe drop and hybridization;

when the slide rack enters the fifth reaction tank 9, the control module controls the liquid injection pump 20 and the liquid injection electromagnetic valve 17 on the liquid injection pipe of the first reagent bottle to automatically inject the sixth reaction liquid in the first reagent bottle into the sixth reaction tank 10, and the control module controls the liquid injection pump 20 and the liquid injection electromagnetic valve 17 on the liquid injection pipe of the second reagent bottle to automatically inject the seventh reaction liquid in the second reagent bottle 16 into the seventh reaction tank 11;

when the slide rack enters the sixth reaction tank 10, the control module controls a liquid injection pump 20 and a liquid injection electromagnetic valve 17 on a liquid injection pipe of the third reagent bottle to automatically inject the eighth reaction liquid in the third reagent bottle into the eighth reaction tank 12;

and (3) eluting after hybridization:

injecting a first eluent into the ninth reaction tank 13, injecting a second eluent into the tenth reaction tank 14, controlling a heating module on the ninth reaction tank 13 by the control module to heat the ninth reaction tank 13 to a preset temperature and keep the preset temperature, heating a tenth reaction tank 14 to the preset temperature and keep the preset temperature by the heating module on the tenth reaction tank 14, stirring the second eluent in the tenth reaction tank 14 until the second eluent is completely dissolved, and controlling a refrigeration module on the tenth reaction tank 14 to reduce the temperature of the tenth reaction tank 14 to be lower than the room temperature by the control module after the second eluent is completely dissolved;

placing the sample slide on which the probe slide and hybridization have been completed on a slide frame 2, and mounting the slide frame 2 on a robot arm 3;

the control module controls the mechanical arm 3 to sequentially send the slide rack 2 into the ninth reaction tank 13 and the tenth reaction tank 14, so that elution after hybridization of the sample slide is completed;

the control module controls the liquid discharge system to discharge the reacted first eluent and second eluent from the ninth reaction tank 13 and the tenth reaction tank 14 respectively, namely, the control module controls the liquid discharge pump and the liquid discharge electromagnetic valve 18 to discharge the first eluent in the ninth reaction tank 13 and the second eluent in the tenth reaction tank 14.

It should be noted that, the sample slide enters each reaction tank and stays for a period of time for reaction, and the specific stay time is determined according to actual requirements.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.