阅读说明：本技术 一种防治红托竹荪黄水病病害的有效药及施药方法 (Effective drug for preventing and treating yellow water disease of dictyophora rubrovolvata and application method ) 是由 田风华 张军 袁潇潇 方晔 彭科琴 谭廷俊 王勇 赵志博 徐彦军 巩玉辉 陈翠翠 于 2021-10-12 设计创作，主要内容包括：本发明提供一种防治红托竹荪黄水病病害的有效药及施药方法,属于食用菌栽培技术领域。本发明的有效药配方如下①中生菌素1.30～1.70g、②甲基硫菌灵0.40～0.60mL、③氨基寡糖素0.40～0.60mL、④丙环唑0.08～0.12mL、⑤咪鲜胺锰盐0.40～0.60g、⑥甲氰菊酯0.80～1.20mL、水0.80～1.20L。本发明的施药过程包括：(1)病害调查和诊断(2)环境条件控制(3)发病竹蛋、竹花清理(4)防治药剂配制(5)防治药剂喷施(6)喷施后栽培管理；本发明能有效控制控由该病原致使红托竹荪引发的黄水病。(The invention provides an effective medicine for preventing and treating yellow water disease of dictyophora rubrovolvata and a medicine application method, belonging to the technical field of edible fungus cultivation. The effective medicine formula of the invention comprises 1.30-1.70 g of zhongshengmycin, 0.40-0.60 mL of thiophanate-methyl, 0.40-0.60 mL of amino-oligosaccharin, 0.08-0.12 mL of propiconazole, 0.40-0.60 g of prochloraz manganese, 0.80-1.20 mL of fenpropathrin and 0.80-1.20L of water. The application process of the invention comprises the following steps: (1) disease investigation and diagnosis (2) environmental condition control (3) diseased bamboo eggs and bamboo flowers cleaning (4) prevention and control agent preparation (5) prevention and control agent spraying (6) cultivation management after spraying; the invention can effectively control and control the yellow water disease caused by the pathogen.)

1. An effective drug for preventing and treating yellow water disease of dictyophora rubrovolvata is characterized in that the formula of the effective drug is as follows:

name (R) Content of effective component Dosage of Middle school bacterin 12% 1.30～1.70g ② thiophanate-methyl 500 g/l 0.40～0.60mL ③ amino-oligosaccharin 5% 0.40～0.60mL Propiconazole 250 g/l 0.08～0.12mL Prochloraz manganese salt 50% 0.40～0.60g Fenpropathrin 20% 0.80～1.20mL Water (W) 0.80～1.20L

。

2. The effective drug for controlling yellow water disease of dictyophora rubrovolvata according to claim 1, characterized in that the formula of the effective drug is as follows:

name (R) Content of effective component Dosage forms Dosage of Middle school bacterin 12% Wettable powder 1.30～1.70g ② thiophanate-methyl 500 g/l Suspending agent 0.40～0.60mL ③ amino-oligosaccharin 5% Aqueous preparation 0.40～0.60mL Propiconazole 250 g/l Emulsifiable concentrate 0.08～0.12mL Prochloraz manganese salt 50% Wettable powder 0.40～0.60g Fenpropathrin 20% Emulsifiable concentrate 0.80～1.20mL Water (W) 0.80～1.20L

。

3. The effective drug for controlling yellow water disease of dictyophora rubrovolvata according to claim 1, characterized in that the formula of the effective drug is as follows:

name (R) Content of effective component Dosage forms Dosage of Middle school bacterin 12% Wettable powder 1.40～1.60g ② thiophanate-methyl 500 g/l Suspending agent 0.45～0.55mL ③ amino-oligosaccharin 5% Aqueous preparation 0.45～0.55mL Propiconazole 250 g/l Emulsifiable concentrate 0.09～0.11mL Prochloraz manganese salt 50% Wettable powder 0.45～0.55g Fenpropathrin 20% Emulsifiable concentrate 0.90～1.10mL Water (W) 0.90～1.10L

。

4. The effective drug for controlling Dictyophora rubrovalvata yellow water disease according to any one of claims 1 to 3, characterized in that the dosing sequence of the effective drug at the time of formulation is: adding zhongshengmycin, thiophanate-methyl, amino-oligosaccharin, propiconazole, prochloraz manganese salt and fenpropathrin into water in sequence.

5. A method for applying an effective drug for preventing and treating yellow water disease of Dictyophora rubrovalvata is characterized by mainly comprising the following steps:

(1) cleaning the bamboo eggs and the bamboo flowers which are attacked: immediately cleaning diseased mushrooms and rotten mushrooms on a mushroom bed after the yellow water disease occurs; before the agent is sprayed, all bamboo eggs which are seriously rotten in the middle and later stages of diseases and dictyophora rubrovolvata with opened bamboo flowers in a cultivation field are comprehensively cleaned so as to reduce the number of pathogens, control the pathogens to continuously infect and prevent the bamboo flowers from being polluted by the agent;

(2) preparing a medicament: according to the proportion, adding zhongshengmycin, thiophanate-methyl, amino-oligosaccharin, propiconazole, prochloraz manganese salt and fenpropathrin into water in corresponding proportion, and stirring or shaking and mixing uniformly;

(3) spraying a liquid medicine: the prepared agent is sprayed on the bamboo egg area with the yellow water disease of Dictyophora rubrovalvata, and the agent is sprayed on the bamboo egg covered by the agent spray cage or the surface of the bamboo egg is suspended by the liquid medicine.

6. The method for applying the effective drug for controlling yellow rot disease of dictyophora rubrovolvata according to claim 5, characterized in that the diagnostic standard for the yellow rot disease before application is as follows:

the early symptom of the yellow water disease is that light red small drops are secreted on the surfaces of the fungus skins of the fruiting bodies and mostly occur at the junction of the bamboo eggs and the soil side of the bamboo eggs, the drops are gradually enlarged and deepened, the drops of exudates of the bamboo eggs begin to turn red, the whole body is yellow water, and the drops spread outwards from the center of the disease and are festered; along with the occurrence of diseases, a large number of springtails are gathered on the surface of the liquid drops, and the springtails suck liquid substances secreted by the bamboo eggs, carry pathogens to adjacent bamboo eggs, cause re-infection and quickly spread to the whole mushroom bed and even a mushroom shed; in the process, various bacteria, fungi, slime bacteria, nematodes, insects, mites and the like are bred, secondary rot diseases occur at the later stage of the yellow water disease of dictyophora rubrovolvata, the surface of the diseased part presents a green mildew symptom, the whole mushroom shed is quickly spread, and the fungus eggs cannot normally grow to shed the mushroom shed; the disease is manifested by the above symptoms and disease phenomena, and can be determined as the yellow water disease.

7. The method for applying the effective drug for controlling yellow rot disease of dictyophora rubrovolvata according to claim 5, characterized in that the environmental conditions after the diagnosis of yellow rot disease are controlled as follows: once the yellow water disease is found, the ventilation needs to be quickly enhanced, the temperature is reduced to 18-23 ℃, the mushroom bed is not humidified, and the soil moisture of the mushroom bed is controlled.

8. The method for applying the effective drug for controlling the yellow rot disease of dictyophora rubrovolvata according to claim 5, characterized in that in the step (3), the shed is closed for 1-2 hours after spraying, the disease control condition is observed 24-36 hours after spraying, the blackening or scabbing of the surface of the diseased tissue is taken as a standard, and if the diseased area is serious, the drug needs to be sprayed again.

9. The method for applying the effective drug for controlling the Dictyophora rubrovalvata yellow water disease according to claim 5, wherein cultivation management after application is as follows: controlling the temperature at 18-23 deg.C, air humidity at 70-80%, and keeping soil humidity until the surface is not dry, and ensuring that water can not be accumulated in the soil of mushroom bed or water is deficient, and water is required to be drained smoothly when planting in forest; timely picking mushrooms every day, and cleaning mushroom beds in time after picking mushrooms to avoid other diseases caused by untimely introduction of insects or microorganisms; the daily administration is carried out by diluting the crude drug at 1/3 concentration and spraying the solution every 25 days.

Technical Field

The invention belongs to the technical field of edible fungus cultivation, and particularly relates to a capsule coated yeast for preventing and treating dictyophora phalloidea

Saccharomycopsis phalluaeAn effective prescription and a medicine application method for causing Dictyophora rubrovalvata yellow water disease caused by pathogens.

Background

Dictyophora rubrovolvata is an important and special rare edible fungus in Guizhou province, and is protected by national geographic identification products, namely Dictyophora phalloidea which belongs to Basidiomycota and Agaric mycota of Agaricuses, Phallales, Phallaceae, DictyophoraPhallus. Because of having beautiful outward appearance, often by its name: "Huang Cai in the fungus" is called "flower of fungus" and "mountain delicacies".

Dictyophora rubrovalvata is planted in large areas in Guizhou, Sichuan and other places in China, and the economic value is high. The health-care food is rich in nutrition, contains 18 amino acids and 20% of protein, 2.6% of crude fat, 60.4% of carbohydrate, vitamins B1, B12 and C, D and the like which are necessary for a human body, can enhance and improve the immune function of the human body, reduce cholesterol and improve the nerve function when being eaten frequently, and has various medicinal functions and wide research and utilization prospects on malignant tumors, hepatitis B, diabetes and the like.

The Dictyophora rubrovalvata is cultivated in an earth covering mode, the period reaches 4-10 months, the Dictyophora rubrovalvata is susceptible to environmental microorganisms, diseases occur, the outer skin of fungus eggs cracks, edema, withering, mold breeding and rotting phenomena occur, the yellow water disease is the most serious disease, the Dictyophora rubrovalvata becomes disaster-caused diseases, the yellow water disease incidence rate reaches 60-80% in partial areas, and the partial areas are even in no use.

In more than 30 years, the disease causes great economic loss and restricts the development of dictyophora industriolata because the pathogenesis of the dictyophora industriolata yellow water disease is not determined and no targeted high-efficiency control measures are taken. The research group defines that the Dictyophora rubrovalvata yellow water disease is Dictyophora rubrovalvata capsule coated yeast for the first timeSaccharomycopsis phalluaeOn the basis of fungal diseases caused by pathogens, research on the occurrence rule of the diseases and establishment of efficient prevention and control measures are carried out, disease early warning and green comprehensive prevention and control technical support are provided for efficient cultivation, quality improvement and synergy of dictyophora rubrovolvata, the industrial revolution of rural areas in Guizhou province is promoted, and the work of poverty elimination and hardness elimination is assisted.

Disclosure of Invention

The technical problem to be solved by the invention is to solve the membrane-covered yeast with dictyophora phalloideaSaccharomycopsis phalluaeProvides an effective medicine and a medicine application method for Dictyophora rubrovalvata yellow water disease caused by fungal pathogens.

The technical scheme of the invention is as follows: an effective drug for preventing and treating yellow water disease of dictyophora rubrovolvata, which comprises the following formula:

name (R)	Content of effective component	Dosage of
			Middle school bacterin	12%	1.30～1.70g
② thiophanate-methyl	500 g/l	0.40～0.60mL
			③ amino-oligosaccharin	5%	0.40～0.60mL
Propiconazole	250 g/l	0.08～0.12mL
			Prochloraz manganese salt	50%	0.40～0.60g
Fenpropathrin	20%	0.80～1.20mL
			Water (W)		0.80～1.20L

Further, the formula of the effective drug of the invention is as follows:

name (R)	Content of effective component	Dosage forms	Dosage of
				Middle school bacterin	12%	Wettable powder	1.30～1.70g
② thiophanate-methyl	500 g/l	Suspending agent	0.40～0.60mL
				③ amino-oligosaccharin	5%	Aqueous preparation	0.40～0.60mL
Propiconazole	250 g/l	Emulsifiable concentrate	0.08～0.12mL
				Prochloraz manganese salt	50%	Wettable powder	0.40～0.60g
Fenpropathrin	20%	Emulsifiable concentrate	0.80～1.20mL
				Water (W)			0.80～1.20L

An effective drug application method for preventing and treating Dictyophora rubrovalvata yellow water disease mainly comprises the following steps:

(1) cleaning the bamboo eggs and the bamboo flowers which are attacked: immediately cleaning diseased mushrooms and rotten mushrooms on a mushroom bed after the yellow water disease occurs; before the agent is sprayed, all bamboo eggs which are seriously rotten in the middle and later stages of diseases and dictyophora rubrovolvata with opened bamboo flowers in a cultivation field are comprehensively cleaned so as to reduce the number of pathogens, control the pathogens to continuously infect and prevent the bamboo flowers from being polluted by the agent;

(2) preparing a medicament: according to the proportion, adding zhongshengmycin, thiophanate-methyl, amino-oligosaccharin, propiconazole, prochloraz manganese salt and fenpropathrin into water in corresponding proportion, and stirring or shaking and mixing uniformly;

(3) spraying a liquid medicine: the prepared agent is sprayed on the bamboo egg area with the yellow water disease of Dictyophora rubrovalvata, and the agent is sprayed on the bamboo egg covered by the agent spray cage or the surface of the bamboo egg is suspended by the liquid medicine.

In the method, once the yellow water disease is found, the ventilation is rapidly enhanced, the temperature is conditionally reduced to 18-23 ℃, the space humidity is reduced, the mushroom bed is not humidified, and the soil humidity of the mushroom bed is controlled.

Further, the greenhouse is closed for 1-2 hours after the pesticide is applied, the disease control condition is observed 24-36 hours after the pesticide is applied, the blackening or scabbing of the surface of the diseased tissue is better controlled, and the pesticide needs to be sprayed again if the diseased area is serious.

Furthermore, after the prevention and treatment agent is sprayed, the temperature is controlled to be 18-23 ℃, the air humidity is controlled to be about 70-80%, the soil humidity is not dry on the surface, and the purposes of low temperature, low humidity and good ventilation are achieved as far as possible. Water can not be accumulated in the mushroom bed soil or water is deficient, and water can be drained smoothly when the mushroom bed is planted in a forest. The mushrooms need to be picked in time every day, and the mushroom beds need to be cleaned in time after the mushrooms are picked, so that other diseases caused by untimely introduction of insects or microorganisms such as mushroom mosquitoes, mushroom flies and the like to the mushrooms are avoided. The spray is sprayed once every 25 days after the 1/3 diluted solution is prepared according to the above drug concentration in daily management. Meanwhile, managers need to carry the pesticide spray can at any time to check the occurrence condition of diseases in time, and the pesticide is sprayed on a small amount of or just occurring bamboo eggs or on recurrent bamboo eggs at fixed points again after prevention, so that the diseases are prevented from happening again.

The inventor points out that: the early symptom of the yellow water disease is that light red small drops are secreted on the surfaces of the fungus skins of the fruiting bodies and are mostly generated at the junction of the bamboo eggs and the soil side of the bamboo eggs (careful observation is needed), then the drops are gradually enlarged and deepened, and the drops of exudates of the bamboo eggs begin to turn red, are yellow water-shaped integrally and spread outwards from the center of disease occurrence and are festered. Along with the occurrence of diseases, a large number of springtails gather on the surface of the liquid drops, the springtails suck liquid substances secreted by bamboo eggs, carry pathogens to adjacent bamboo eggs, cause re-infection and quickly spread to the whole mushroom bed; in the process, various bacteria, fungi, slime, nematodes, insects (mainly flea worms, mushroom mosquitoes, mushroom flies, slugs), mites and the like are bred, secondary rot diseases of dictyophora rubrovolvata yellow water disease in the later period of pathogenesis are caused, the surface of the pathogenesis part presents a green mildew symptom, the whole mushroom shed is quickly spread, and the fungus eggs cannot normally grow to shed. The disease is manifested by the above symptoms and disease phenomena, and can be determined as the yellow water disease.

The advantages of the invention are as follows: on the premise that the first invention identifies the pathogen of Dictyophora rubrovolvata yellow water disease as the first person, according to the pathogen Dictyophora rubrovolvata envelope-covering yeastSaccharomycopsis phalluaeThe screening of the effective control agent provided by the invention can effectively control the continuous infection and the expanded infection of the Dictyophora rubrovalvata yellow water disease according to the application steps, and can also effectively prevent the occurrence of diseases. Meanwhile, the formula medicament in the control medicament can be directly purchased and obtained in the market, has simple medicament preparation process and easy operation, and is suitable for practical application of almost all units such as individual farmers, large, medium and small enterprises and the like.

The invention has the beneficial effects that: preventing diseases. Secondly, the pathogenic bacteria can be prevented from continuously infecting the dictyophora rubrovolvata, the diseased parts of the bamboo eggs are changed into 'dry scar' shapes from water stain shapes, and the bamboo eggs recover physiological health and continue to grow and develop. The expanding infection of pathogens to dictyophora rubrovolvata is prevented, and when the pathogens are sprayed, insects including springtails, mushroom mosquitoes, mushroom flies, slugs, mites and the like are also killed, so that the carrying and propagation of the pathogens by pests are greatly reduced, and the expanding of dictyophora rubrovolvata yellow rot is prevented. The application of the medicine formula provided by the invention can obtain effective prevention and treatment effect even under the condition that the yellow water disease of dictyophora rubrovolvata is serious, and the bamboo flowers can still bloom after the physiological function of the dictyophora rubrovolvata is gradually recovered, thereby realizing the yield.

Drawings

FIG. 1 is a comparison between before and after application at the initial stage of occurrence of a disease in example 1 of the present invention;

FIG. 2 is a comparison of before and after application in the middle and later stages of disease occurrence in example 2 of the present invention;

FIG. 3 is a graph showing a comparison of the effect on fruiting of the presence or absence of control after the occurrence of a disease in example 3 of the present invention.

Detailed Description

Aims at the dictyophora indusiata capsule coated yeastSaccharomycopsis phalluaeAn effective medicine for treating Dictyophora rubrovalvata yellow water disease caused by fungal pathogen and its application method are disclosedThe preparation method comprises the following steps:

the formula is as follows:

the application step comprises:

1. disease investigation and diagnosis: the early symptom of the yellow water disease is that light red small drops are secreted on the surface of the fungus skin of the fruiting body and are mostly generated at the junction of the bamboo eggs and the soil side of the bamboo eggs (careful observation is needed), then the drops are gradually enlarged and deepened, and the drops of exudates of the bamboo eggs begin to turn red, are yellow water-shaped as a whole and spread outwards from the center of the disease and are festered. Along with the occurrence of diseases, a large number of springtails gather on the surface of the liquid drops, the springtails suck liquid substances secreted by bamboo eggs, carry pathogenic agents on adjacent bamboo eggs, cause re-infection and quickly spread to the whole mushroom bed; in the process, various bacteria, fungi, slime, nematodes, insects (mainly flea worms, mushroom mosquitoes, mushroom flies and slugs), mites and the like are bred, secondary saprophytic diseases are caused in the later period of the yellow water disease of dictyophora rubrovolvata, the disease surface presents a green mold symptom, the whole mushroom shed is quickly spread, and the fungus eggs cannot normally grow to shed. The disease is manifested by the above symptoms and disease phenomena, and can be determined as the yellow water disease.

2. And (3) controlling environmental conditions: once the yellow water disease is found, the ventilation is rapidly enhanced, the temperature is conditionally reduced to 18-23 ℃, the space humidity is reduced, the mushroom bed is not humidified, and the soil of the mushroom bed is controlled to be moist.

3. Cleaning the bamboo eggs and the bamboo flowers which are attacked: the method is characterized in that diseased mushrooms and rotten mushrooms on mushroom beds are cleaned immediately after the yellow water disease occurs, particularly, all bamboo eggs which are seriously rotten in the middle and later stages of disease occurrence and dictyophora rubrovolvata with bamboo flowers already bloomed in a cultivation field are cleaned completely before the agent is sprayed, so that the pathogen number is reduced, the continuous infection of the bamboo eggs is controlled, and the bamboo flowers are prevented from being polluted by the agent.

4. Preparing a control medicament: according to the formula provided by the invention, zhongshengmycin, thiophanate-methyl, amino-oligosaccharin, propiconazole, prochloraz manganese and fenpropathrin are added into water according to corresponding proportion in sequence, and are stirred or vibrated to be uniformly mixed.

5. Spraying a control agent: the prepared agent is utilized to carry out comprehensive agent spraying on the bamboo egg area with the onset of the Dictyophora rubrovolvata and the vicinity thereof by means of agricultural tools such as a manual pneumatic spray bottle, an electric knapsack sprayer and the like under the protection of sanitary protective equipment such as a mask, gloves and the like, and the agent is sprayed to the bamboo egg covered by the agent spray cage or the surface of the bamboo egg is suspended by the liquid medicine. The shed can be closed for 1-2 hours after spraying, the disease control condition is observed 24-36 hours after spraying, the blackening or scabbing of the surface of the diseased tissue is better controlled, and the pesticide is sprayed again if the diseased area is serious.

6. And (3) cultivation management after spraying: after the prevention and treatment agent is sprayed, the temperature is controlled to be 18-23 ℃, the air humidity is controlled to be about 70-80%, the soil humidity is not dry on the surface, and the purposes of low temperature, low humidity and good ventilation are achieved as far as possible. Water can not be accumulated in the mushroom bed soil or water is deficient, and water can be drained smoothly when the mushroom bed is planted in a forest. The mushrooms need to be picked in time every day, and the mushroom beds need to be cleaned in time after the mushrooms are picked, so that other diseases caused by untimely introduction of insects or microorganisms such as mushroom mosquitoes, mushroom flies and the like to the mushrooms are avoided. The spray is sprayed once every 25 days after the 1/3 diluted solution is prepared according to the above drug concentration in daily management. Meanwhile, managers need to check the occurrence condition of the diseases in time, and sometimes spray the medicine at fixed points again when encountering a small amount of or just occurring bamboo eggs or recurrent bamboo eggs after prevention and treatment, so as to avoid the reoccurrence of the diseases.

Example 1:

the application step comprises:

1. disease investigation and diagnosis: a large number of light red small drops are secreted and exuded at the junction of Dictyophora rubrovolvata and on the soil-near side of the bamboo egg (carefully observed), and the color and volume of the small drops gradually deepen along with the continuous disease. The disease in this case was determined to be Dictyophora rubrovalvata yellow water disease.

2. And (3) controlling environmental conditions: if the yellow water disease is found, the ventilation is rapidly enhanced, the temperature is conditionally reduced to 18-23 ℃, the space humidity is reduced, the mushroom bed is not humidified, and the soil of the mushroom bed is controlled to be moist.

3. Cleaning the bamboo eggs and the bamboo flowers which are attacked: the method is characterized in that diseased mushrooms and rotten mushrooms on mushroom beds are cleaned immediately after the yellow water disease occurs, particularly, all bamboo eggs which are seriously rotten in the middle and later stages of disease occurrence and dictyophora rubrovolvata with bamboo flowers already bloomed in a cultivation field are cleaned completely before the agent is sprayed, so that the pathogen number is reduced, the continuous infection of the bamboo eggs is controlled, and the bamboo flowers are prevented from being polluted by the agent.

4. Preparing a control medicament: according to the formula provided by the invention, 1.50g of zhongshengmycin, 0.50mL of thiophanate-methyl, 0.50mL of amino-oligosaccharin, 0.10mL of propiconazole, 0.50g of prochloraz manganese and 1.00mL of fenpropathrin are sequentially added into 1.00L of water, and the mixture is stirred or vibrated to be uniformly mixed.

5. Spraying a control agent: the prepared agent is utilized to carry out comprehensive agent spraying on the bamboo egg area with the onset of the Dictyophora rubrovolvata and the vicinity thereof by means of agricultural tools such as a manual pneumatic spray bottle, an electric knapsack sprayer and the like under the protection of sanitary protective equipment such as a mask, gloves and the like, and the agent is sprayed to the bamboo egg covered by the agent spray cage or the surface of the bamboo egg is suspended by the liquid medicine. The shed can be closed for 1-2 hours after spraying, the disease control condition is observed 24-36 hours after spraying, the blackening or scabbing of the surface of the diseased tissue is better controlled, and the pesticide is sprayed again if the diseased area is serious.

6. And (3) cultivation management after spraying: after the prevention and treatment agent is sprayed, the temperature is controlled to be 18-23 ℃, the air humidity is about 75%, the soil humidity is not dry on the surface, and the purposes of low temperature, low humidity and good ventilation are achieved as far as possible. Water can not be accumulated in the mushroom bed soil or water is deficient, and water can be drained smoothly when the mushroom bed is planted in a forest. The mushrooms need to be picked in time every day, and the mushroom beds need to be cleaned in time after the mushrooms are picked, so that other diseases caused by untimely introduction of insects or microorganisms such as mushroom mosquitoes, mushroom flies and the like to the mushrooms are avoided. The spray is sprayed once every 25 days after the 1/3 diluted solution is prepared according to the above drug concentration in daily management. Meanwhile, managers need to carry the pesticide spraying pot at any time, and the pesticide spraying is carried out on a small amount of bamboo eggs which are just generated or recur after the pesticide spraying, so that the recurrence of diseases is avoided.

After the effective medicine is applied, the effect is shown as figure 1, the left figure is that before prevention and treatment, a large amount of light red small drops seep from the surface of the bamboo eggs; the right picture shows that after the control, the disease is effectively controlled, yellow liquid drops on the surface are not formed again, and the surface of the bamboo egg shows dry and comfortable signs.

Example 2:

the application step comprises:

1. disease investigation and diagnosis: the surface of Dictyophora rubrovalvata bamboo egg presents water stain-shaped scab with the area of about 4mm multiplied by 4mm, tiny liquid drops are accompanied in the center of the scab, and rotten symptoms appear on the edge of the scab. The disease in this case was determined to be Dictyophora rubrovalvata yellow water disease.

2. And (3) controlling environmental conditions: if the yellow water disease is found, the ventilation is rapidly enhanced, the temperature is conditionally reduced to 18-23 ℃, the space humidity is reduced, the mushroom bed is not humidified, and the soil of the mushroom bed is controlled to be moist.

3. Cleaning the bamboo eggs and the bamboo flowers which are attacked: the method is characterized in that diseased mushrooms and rotten mushrooms on mushroom beds are cleaned immediately after the yellow water disease occurs, particularly, all bamboo eggs which are seriously rotten in the middle and later stages of disease occurrence and dictyophora rubrovolvata with bamboo flowers already bloomed in a cultivation field are cleaned completely before the agent is sprayed, so that the pathogen number is reduced, the continuous infection of the bamboo eggs is controlled, and the bamboo flowers are prevented from being polluted by the agent.

4. Preparing a control medicament: according to the formula provided by the invention, 1.30g of zhongshengmycin, 0.40mL of thiophanate-methyl, 0.40mL of amino-oligosaccharin, 0.08mL of propiconazole, 0.40g of prochloraz manganese and 0.80mL of fenpropathrin are sequentially added into 0.80L of water, and the mixture is stirred or vibrated to be uniformly mixed.

5. Spraying a control agent: the prepared agent is utilized to carry out comprehensive agent spraying on the bamboo egg area with the onset of the Dictyophora rubrovolvata and the vicinity thereof by means of agricultural tools such as a manual pneumatic spray bottle, an electric knapsack sprayer and the like under the protection of sanitary protective equipment such as a mask, gloves and the like, and the agent is sprayed to the bamboo egg covered by the agent spray cage or the surface of the bamboo egg is suspended by the liquid medicine. The shed can be closed for 1-2 hours after spraying, the disease control condition is observed 24-36 hours after spraying, the blackening or scabbing of the surface of the diseased tissue is better controlled, and the pesticide is sprayed again if the diseased area is serious.

6. And (3) cultivation management after spraying: after the prevention and treatment agent is sprayed, the temperature is controlled to be 18-23 ℃, the air humidity is controlled to be about 75-80%, the soil humidity is not dry on the surface, and the purposes of low temperature, low humidity and good ventilation are achieved as far as possible. Water can not be accumulated in the mushroom bed soil or water is deficient, and water can be drained smoothly when the mushroom bed is planted in a forest. The mushrooms need to be picked in time every day, and the mushroom beds need to be cleaned in time after the mushrooms are picked, so that other diseases caused by untimely introduction of insects or microorganisms such as mushroom mosquitoes, mushroom flies and the like to the mushrooms are avoided. The spray is sprayed once every 25 days after the 1/3 diluted solution is prepared according to the above drug concentration in daily management. Meanwhile, managers need to carry the pesticide spraying pot at any time, and the pesticide spraying is carried out on a small amount of bamboo eggs which are just generated or recur after the pesticide spraying, so that the recurrence of diseases is avoided.

After the effective medicine is applied, the effect is shown in figure 2, the left figure is before prevention, the bamboo eggs start to rot from the center of the yellow water disease to the outer edge, and the yellow water drops flow through the trace; the right picture shows that after the prevention and treatment, the disease part has already been dry scar, the yellow water flowing trace disappears, and the yellow water disease is effectively controlled.

Example 3:

the application step comprises:

1. disease investigation and diagnosis: a large amount of yellow water seeps out of the surface of the Dictyophora rubrovalvata bamboo eggs, and the eggs are in a water stain shape and accompanied with obvious rotten symptoms. The breeding of mushroom mosquitoes, mites and the like is accompanied around the bamboo eggs, and diseases spread in the whole mushroom shed. The disease in this case was determined to be Dictyophora rubrovalvata yellow water disease.

2. And (3) controlling environmental conditions: once the yellow water disease is found, the ventilation is rapidly enhanced, the temperature is conditionally reduced to 18-23 ℃, the space humidity is reduced, the mushroom bed is not humidified, and the soil of the mushroom bed is controlled to be moist.

3. Cleaning the bamboo eggs and the bamboo flowers which are attacked: the method is characterized in that diseased mushrooms and rotten mushrooms on mushroom beds are cleaned immediately after the yellow water disease occurs, particularly, all bamboo eggs which are seriously rotten in the middle and later stages of disease occurrence and dictyophora rubrovolvata with bamboo flowers already bloomed in a cultivation field are cleaned completely before the agent is sprayed, so that the pathogen number is reduced, the continuous infection of the bamboo eggs is controlled, and the bamboo flowers are prevented from being polluted by the agent.

4. Preparing a control medicament: according to the formula provided by the invention, 1.70g of zhongshengmycin, 0.60mL of thiophanate-methyl, 0.60mL of amino-oligosaccharin, 0.12mL of propiconazole, 0.60g of prochloraz manganese and 1.20mL of fenpropathrin are sequentially added into 1.20L of water, and the mixture is stirred or vibrated to be uniformly mixed.

5. Spraying a control agent: the prepared agent is utilized to carry out comprehensive agent spraying on the bamboo egg area with the onset of the Dictyophora rubrovolvata and the vicinity thereof by means of agricultural tools such as a manual pneumatic spray bottle, an electric knapsack sprayer and the like under the protection of sanitary protective equipment such as a mask, gloves and the like, and the agent is sprayed to the bamboo egg covered by the agent spray cage or the surface of the bamboo egg is suspended by the liquid medicine. The shed can be closed for 1-2 hours after spraying, the disease control condition is observed 24-36 hours after spraying, the blackening or scabbing of the surface of the diseased tissue is better controlled, and the pesticide is sprayed again if the diseased area is serious.

6. And (3) cultivation management after spraying: after the prevention and treatment agent is sprayed, the temperature is controlled to be 18-23 ℃, the air humidity is controlled to be about 70-80%, the soil humidity is not dry on the surface, and the purposes of low temperature, low humidity and good ventilation are achieved as far as possible. Water can not be accumulated in the mushroom bed soil or water is deficient, and water can be drained smoothly when the mushroom bed is planted in a forest. The mushrooms need to be picked in time every day, and the mushroom beds need to be cleaned in time after the mushrooms are picked, so that other diseases caused by untimely introduction of insects or microorganisms such as mushroom mosquitoes, mushroom flies and the like to the mushrooms are avoided. The spray is sprayed once every 25 days after the 1/3 diluted solution is prepared according to the above drug concentration in daily management. Meanwhile, managers need to carry the pesticide spraying pot at any time, and the pesticide spraying is carried out on a small amount of bamboo eggs which are just generated or recur after the pesticide spraying, so that the recurrence of diseases is avoided.

After the diseases occur, the fruiting is affected after the effective medicine is applied or not, the effect is shown in figure 3, the left figure shows that no prevention and control measures are taken after the diseases occur, the yellow water disease is aggravated, the bamboo eggs are festered in a large area, and the breeding of mould, mushroom mosquitoes and the like is accompanied, so that the bamboo eggs in the whole area are completely harvested; the right picture shows that prevention and treatment measures are taken in time after the disease occurs, the disease is effectively controlled, the bamboo eggs are recovered to be healthy, the epidermis shows dry and dry scars, and the bamboo eggs can bloom normally.