阅读说明：本技术 一种防治白盾蚧类害虫的农药增效剂和农药组合物 (Pesticide synergist and pesticide composition for preventing and treating white pelecypyriasis insects ) 是由 程东美 何景超 张志祥 周易 于 2021-08-17 设计创作，主要内容包括：本发明涉及农药技术领域,特别是涉及一种防治白盾蚧类害虫的农药增效剂和农药组合物,该农药增效剂所述农药增效剂包括植物油,所述植物油选自肉桂油、巴豆油、青蒿油、八角茴香油、香茅油中的一种或两种以上的混合物,该农药增效剂能有效地溶解白盾蚧类害虫的蜡沁物和蚧壳,提高杀虫药剂的杀虫速度和效果,并且具有抗耐药的优点；该农药组合物中的农药增效剂与白盾蚧类害虫杀虫药剂有效成分之间具有很好的协同增效作用,但无交互抗性,可以有效保持杀虫药剂的杀虫效果。(The invention relates to the technical field of pesticides, in particular to a pesticide synergist and a pesticide composition for preventing and treating white pellicularia, wherein the pesticide synergist comprises vegetable oil, the vegetable oil is one or a mixture of more than two of cinnamon oil, croton oil, sweet wormwood oil, anise oil and citronella oil, and the pesticide synergist can effectively dissolve waxy epipremnum of the white pellicularia and scale shells, improves the insecticidal speed and effect of an insecticide and has the advantage of drug resistance; the pesticide synergist in the pesticide composition has good synergistic interaction with the active ingredients of the pelteobagrus insect pesticide, but has no cross resistance, and can effectively maintain the insecticidal effect of the pesticide.)

1. A pesticide synergist for preventing and treating white pelecypyriasis insects is characterized in that: the pesticide synergist comprises vegetable oil, wherein the vegetable oil is selected from one or a mixture of more than two of cinnamon oil, croton oil, sweet wormwood oil, anise oil and citronella oil.

2. The pesticide synergist for controlling pelecypyriasis alba pests according to claim 1, characterized in that: the white pelecypyriasis insect is white pelecypyriasis insect and pseudowhite pelecypyriasis insect.

3. The pesticide synergist for controlling pelecypyriasis alba pests according to claim 1, characterized in that: the cinnamon oil, the croton oil, the sweet wormwood oil, the star anise oil and the citronella oil are plant essential oils.

4. The pesticide synergist for controlling pelecypyriasis alba pests according to claim 1, characterized in that: the pesticide synergist comprises the following raw materials in percentage by weight:

2 to 80 percent of vegetable oil

2 to 30 percent of surfactant

5 to 80 percent of solvent.

5. The pesticide synergist for controlling pelecypyriasis alba pests according to claim 4, characterized in that: the solvent is one or a mixture of more than two of acetonitrile, ethanol and acetone.

6. The pesticide synergist for controlling pelecypyriasis alba pests according to claim 4, characterized in that: the surfactant is one or a mixture of more than two of tween 80, castor oil, dimethyl sulfoxide, sorbitan monooleate and sucrose fatty acid ester.

7. A pesticidal composition characterized by: comprises a pelecypyrium pelteobagrus pest insecticide and the pesticide synergist of any one of claims 1 to 6.

8. The pesticidal composition according to claim 7, characterized in that: the Pestegia baishanensis pest insecticide is one or a mixture of more than two of cyhalothrin, spirotetramat and bifenazate.

9. The pesticidal composition according to claim 7, characterized in that: the weight percentage of the pesticide synergist in the pesticide composition is 2-90%.

Technical Field

The invention relates to the technical field of pesticides, in particular to a pesticide synergist and a pesticide composition for preventing and treating white pelecypyriasis.

Background

The pelagia insect belongs to Insecta, homoptera, and Lecanida, and has a true scale on the surface, which is composed of the previous-age periostracum and its secretion, and the main component is wax. Except for male adults, female and nymphs live in a fixed life. The female adults are mostly covered by shield-like mesochites, which are separated from the polypide.

Pelecyporus pelecypus and pseudopelecyporus pelecypus are both typical pelecyporus pelecypus, widely distributed around the world, and are harmful to gardens, vegetables, flowers, traditional Chinese medicinal materials and parts of crops. Nymphs and female adults are fixed on the branches and leaves to prick and absorb plant juice, so that the damage period is long. The pest bodies on trunks, branches or leaves of the damaged plants are densely distributed, the growth of the plants is seriously influenced, and the yield and the quality of economic crops such as fruit trees, tea leaves and the like are influenced. When the harm is serious, the plant grows slowly, the tree vigor is weak, the tender branches are dry, and even the whole plant dies. The pelteobagrus has thick wax shells in wax secretion form on the surface, and the liquid medicine is not easy to permeate, so that the prevention and treatment effect is poor. In order to improve the control effect, the dosage or the application frequency is increased in production, so that the problems of increase of pest resistance, serious residue, environmental pollution and the like occur.

The plant essential oil is an important secondary metabolite existing in different tissues of plants, is easy to dissolve in an organic solvent, and has a good dissolving effect on various waxiness. The current research shows that the plant essential oil has biological activity to various pests and pathogenic bacteria and has synergistic effect to various pesticides. However, the plant essential oils currently used for controlling pests are mainly terpenoids, aromatic compounds, aliphatic compounds and nitrogen-containing and sulfur-containing compounds, and the plant essential oils cannot have good synergistic effect on the scale insects, so that the scale insects cannot be better controlled.

Disclosure of Invention

The first purpose of the invention is to provide a pesticide synergist for preventing and controlling the white pelecypyriasis insect, which can effectively dissolve the wax insecticide of the white pelecypyriasis insect, improve the insecticidal speed and effect of the pesticide and have the advantage of drug resistance.

The first purpose of the invention is realized by the following technical scheme:

the pesticide synergist comprises vegetable oil, wherein the vegetable oil is one or a mixture of more than two of cinnamon oil, croton oil, sweet wormwood oil, anise oil and citronella oil.

The principle of the pesticide synergist for preventing and treating the pelecypocladium leucotrichum is as follows: the cinnamon oil, the croton oil, the sweet wormwood oil, the star anise oil and the citronella oil are rich in anethole, D-limonene, cinnamaldehyde and methyl salicylate, and the anethole, the D-limonene, the cinnamaldehyde and the methyl salicylate have excellent dissolving effect on waxy secretions of the white peltry insects, so that the cinnamon oil, the croton oil, the sweet wormwood oil, the star anise oil and the citronella oil in the vegetable oil can reduce or eliminate the waxy secretions of the white peltry insects, the penetration effect of the insecticide on the body walls of the white peltry insects is improved, the insecticide is enabled to be rapidly contacted with insects, and the prevention and control effect is improved; and the cinnamon oil not only has a dissolving effect, but also has a poisoning effect on the white pelecypodium pests.

Further, the pelecypoid insects (Aulacaspis) are insects of the genus pelecypus and insects of the genus Pseudopelecypocladium (Pseudoaulacaspis).

Further, the cinnamon oil, the croton oil, the artemisia oil, the star anise oil and the citronella oil are plant essential oils. The plant essential oil has more effective components, and is more beneficial to improving the synergistic effect.

Further, the pesticide synergist comprises the following raw materials in percentage by weight:

2 to 80 percent of vegetable oil

2 to 30 percent of surfactant

5 to 80 percent of solvent.

The surfactant can reduce the surface tension of the pesticide synergist and improve the adhesive force of the pesticide synergist, thereby improving the control effect; the solvent can uniformly mix the raw material components.

Further, the solvent is one or a mixture of any two or more of acetonitrile, ethanol and acetone. Acetonitrile, ethanol and acetone are used as solvents to have better dissolving effects on cinnamon oil, the croton oil, the artemisia oil, the star anise oil and the citronella oil.

Further, the surfactant is one or a mixture of any two or more of tween 80, castor oil, dimethyl sulfoxide, sorbitan monooleate and sucrose fatty acid ester. These surfactants are cheaper and more readily available and are effective in reducing surfactant levels.

Furthermore, the pesticide synergist for preventing and treating the white pelecypyriasis insects can be used for preventing and treating the white pelecypyriasis insects of fruit trees, vegetables, tea, traditional Chinese medicinal materials and various crops.

The pesticide synergist for preventing and treating the white pelecypyriasis insects has the following beneficial effects:

(1) the cinnamon oil, the croton oil, the sweet wormwood oil, the star anise oil and the citronella oil are rich in anethole, D-limonene, cinnamaldehyde and methyl salicylate, and the anethole, the D-limonene, the cinnamaldehyde and the methyl salicylate have excellent dissolving effect on waxy secretions of the white peltry insects, so that the cinnamon oil, the croton oil, the sweet wormwood oil, the star anise oil and the citronella oil in the vegetable oil can reduce or eliminate waxy secretions of the white peltry insects, the penetration effect of the insecticide on the body walls of the white peltry insects is improved, the insecticide is enabled to be in contact with insect bodies quickly, and the prevention and control effect is improved; and the cinnamon oil not only has a dissolving effect, but also has a poisoning effect on the white pelecypodium pests.

(2) The vegetable oil provided by the invention can eliminate waxy secretions of the white pelage insects, reduce the adsorption and retention of the waxy secretions on the surface of the pelage insects to fat-soluble insecticide, increase the contact speed and contact surface of the insecticide and the insect body, improve the dosage of the insect body, and further improve the action speed and the prevention and treatment effect of the insecticide on the white pelage insects.

(3) The pesticide synergist provided by the invention improves the insecticidal effect by dissolving the paradoxus pelteobagrus insect wax secretion, is not easy to generate drug resistance, can effectively reduce the using amount of an insecticide, prevents the pelteobagrus insect from generating drug resistance to the pesticide, and achieves the purposes of reducing the amount and improving the efficiency.

(4) The pesticide synergist belongs to vegetable oil, can reduce the surface tension of the pesticide after being mixed with the pesticide synergist, increases the expanded diameter and the residence time of the pesticide on the surface of a plant, increases the contact probability of the nymph which is hatched initially and the pesticide, and improves the pesticide effect.

(5) The pesticide synergist disclosed by the invention is prepared from plant extracts, is safe to people and livestock, and is a safe and residue-free pesticide auxiliary agent, and the environment is not polluted.

(6) The invention has the advantages of abundant natural resources of raw materials, simple processing and low requirement on equipment, and is suitable for large-scale production and application.

The second purpose of the invention is realized by the following technical scheme:

provides a pesticide composition, which comprises a peltate insect pesticide and the pesticide synergist.

The reaction principle of the raw materials is as follows: the cinnamon oil, the croton oil, the sweet wormwood oil, the star anise oil and the citronella oil are rich in anethole, D-limonene, cinnamaldehyde and methyl salicylate, and the anethole, the D-limonene, the cinnamaldehyde and the methyl salicylate have excellent dissolving effect on waxy secretions of the white peltry insects, so that the cinnamon oil, the croton oil, the sweet wormwood oil, the star anise oil and the citronella oil in the vegetable oil can reduce or eliminate the waxy secretions of the white peltry insects, the penetration effect of the insecticide on the body walls of the white peltry insects is improved, the insecticide is enabled to be rapidly contacted with insects, and the prevention and control effect is improved; the synergistic effect is generated between the pesticide synergist and the pelteobagrus insect pesticide, and the synergistic effect can effectively increase the insecticidal effect when the pesticide synergist and the pelteobagrus insect pesticide are combined with each other and compared with the single use of the pesticide synergist and the pelteobagrus insect pesticide.

Further, the Pestegia baibeiensis insecticide is one or a mixture of more than two of cyhalothrin, spirotetramat and bifenazate. Cyhalothrin, spirotetramat and bifenazate are low-toxic pesticides and are beneficial to environmental protection.

Further, the weight percentage of the pesticide synergist in the pesticide composition is 2-90%.

The pesticide composition has the beneficial effects that:

(1) the pesticide synergist can dissolve wax secretion outside the body wall of the white pelecypodium pest, increase the penetration of the pesticide to the body wall of the white pelecypodium, and improve the pesticide effect of the pesticide.

(2) The pesticide synergist has good synergistic interaction with the active ingredients of the pesticide, but has no cross resistance, and can effectively maintain the insecticidal effect of the pesticide.

Drawings

The invention is further illustrated by means of the attached drawings, but the embodiments in the drawings do not constitute any limitation to the invention, and for a person skilled in the art, other drawings can be derived on the basis of the following drawings without inventive effort.

Fig. 1 is a comparison graph of the dissolution effects of cinnamon essential oil, croton essential oil, sweet wormwood essential oil, star anise essential oil and citronella essential oil on the wax secretion of the female adult of the gecko bainieri in experiment 1, wherein the concentration of the vegetable oil and the 95% alcohol is 1: 1.

Fig. 2 is a comparison graph of the dissolution effects of cinnamon essential oil, croton essential oil, sweet wormwood essential oil, star anise essential oil and citronella essential oil on the wax secretion of the female adult of the gecko bainieri in experiment 1, wherein the concentration of the vegetable oil and the 95% alcohol is 1: 10.

Detailed Description

The invention is further described with reference to the following examples and the accompanying drawings.

Example 1

The embodiment discloses a pesticide synergist for preventing and controlling white peltry pests, which comprises vegetable oil, wherein the vegetable oil is selected from a mixture of cinnamon oil, croton oil and sweet wormwood oil.

The pesticide synergist for preventing and treating the white pelecypyriasis insects has the following effects: the cinnamon oil, the croton oil, the sweet wormwood oil, the star anise oil and the citronella oil have a remarkable dissolving effect on the waxy secretions of the white peltry insects, the protection effect of the waxy secretions on insect bodies can be reduced or eliminated, the adsorption and retention of the waxy secretions on the white peltry insects on a fat-soluble insecticide are reduced, the contact speed and the contact surface of a medicament and the insect bodies are increased, the dosage of the insect bodies is increased, and the action speed and the prevention and control effect of the insecticide on the white peltry insects are remarkably improved. Moreover, after the vegetable oil and the composition in the pesticide synergist are mixed with the pesticide, the surface tension of the liquid medicine can be reduced, the expanding diameter and the residence time of the liquid medicine on the surface of the plant can be increased, and the contact probability of the initially hatched nymph and the pesticide can be increased.

In this embodiment, the pelecypyriasis alba insects are pelecypyriasis alba insects and pseudopelecypyriasis alba insects.

In this embodiment, the cinnamon oil, the croton oil, the artemisia oil, the star anise oil and the citronella oil are plant essential oils.

In the embodiment, the pesticide synergist comprises the following raw materials in percentage by weight:

2% of vegetable oil, 30% of surfactant and 68% of solvent.

In this example, the solvent is a mixture of acetonitrile.

In this example, the surfactant is a mixture of tween 80 and castor oil.

Example 2

The embodiment discloses a pesticide synergist for preventing and treating white pellicularia pests, which comprises vegetable oil, wherein the vegetable oil is a mixture of sweet wormwood oil, star anise oil and citronella oil.

The pesticide synergist for preventing and treating the white pelecypyriasis insects has the following effects: the cinnamon oil, the croton oil, the sweet wormwood oil, the star anise oil and the citronella oil have a remarkable dissolving effect on the waxy secretions of the white peltry insects, the protection effect of the waxy secretions on insect bodies can be reduced or eliminated, the adsorption and retention of the waxy secretions on the white peltry insects on a fat-soluble insecticide are reduced, the contact speed and the contact surface of a medicament and the insect bodies are increased, the dosage of the insect bodies is increased, and the action speed and the prevention and control effect of the insecticide on the white peltry insects are remarkably improved. Moreover, after the vegetable oil and the composition in the pesticide synergist are mixed with the pesticide, the surface tension of the liquid medicine can be reduced, the expanding diameter and the residence time of the liquid medicine on the surface of the plant can be increased, and the contact probability of the initially hatched nymph and the pesticide can be increased.

In this embodiment, the pelecypyriasis alba insects are pelecypyriasis alba insects and pseudopelecypyriasis alba insects.

In this embodiment, the cinnamon oil, the croton oil, the artemisia oil, the star anise oil and the citronella oil are plant essential oils.

In the embodiment, the pesticide synergist comprises the following raw materials in percentage by weight:

80% of vegetable oil, 15% of surfactant and 5% of solvent.

In this example, the solvent is acetone.

In this example, the surfactant is a mixture of tween 80, castor oil, dimethyl sulfoxide, sorbitan monooleate, and sucrose fatty acid ester.

Example 3

The embodiment discloses a pesticide synergist for preventing and controlling white peltry pests, which comprises vegetable oil, wherein the vegetable oil is selected from a mixture of cinnamon oil, croton oil, sweet wormwood oil, star anise oil and citronella oil.

The pesticide synergist for preventing and treating the white pelecypyriasis insects has the following effects: the cinnamon oil, the croton oil, the sweet wormwood oil, the star anise oil and the citronella oil have a remarkable dissolving effect on the waxy secretions of the white peltry insects, the protection effect of the waxy secretions on insect bodies can be reduced or eliminated, the adsorption and retention of the waxy secretions on the white peltry insects on a fat-soluble insecticide are reduced, the contact speed and the contact surface of a medicament and the insect bodies are increased, the dosage of the insect bodies is increased, and the action speed and the prevention and control effect of the insecticide on the white peltry insects are remarkably improved. Moreover, after the vegetable oil and the composition in the pesticide synergist are mixed with the pesticide, the surface tension of the liquid medicine can be reduced, the expanding diameter and the residence time of the liquid medicine on the surface of the plant can be increased, and the contact probability of the initially hatched nymph and the pesticide can be increased.

In this embodiment, the pelecypomus pest is a pelecypomus and/or pelecypomus pseudopelecypomus pest.

In this embodiment, the cinnamon oil, the croton oil, the artemisia oil, the star anise oil and the citronella oil are plant essential oils.

In the embodiment, the pesticide synergist comprises the following raw materials in percentage by weight:

18% of vegetable oil, 2% of surfactant and 80% of solvent.

In this embodiment, the solvent is one or a mixture of two or more of acetonitrile, ethanol, and acetone.

In this embodiment, the surfactant is a mixture of dimethyl sulfoxide, sorbitan monooleate, and sucrose fatty acid ester.

Example 4

The embodiment discloses a pesticide composition, which comprises a pelecypyriasis alba pest insecticide and the pesticide synergist in the embodiment 1.

The pesticide composition has the following effects and effects: the cinnamon oil, the croton oil, the sweet wormwood oil, the star anise oil and the citronella oil have a remarkable dissolving effect on the waxy secretions of the white pelteobagrus pests, can reduce or eliminate the protective effect of the waxy secretions on the pests, and improve the insecticidal effect of the insecticide; the vegetable oil of the pesticide synergist and the effective components of the pesticide have good synergistic interaction effect, but no cross resistance, and the insecticidal effect of the pesticide can be effectively maintained.

In this embodiment, the insecticide for pelteobagrus insect is a mixture of cyhalothrin and bifenazate.

In this example, the weight percentage of the pesticide synergist in the pesticide composition was 2%.

Example 5

The embodiment discloses a pesticide composition, which comprises a pelecypyriasis alba pest insecticide and the pesticide synergist in the embodiment 2.

The pesticide composition has the following effects and effects: the sweet wormwood oil and the croton oil can dissolve wax secretion outside the body wall of the cycado cyrtonema pekinensis, and the croton oil increases the penetration of the insecticide on the body wall of the cycado cyrtonema pekinensis and improves the insecticidal effect of the insecticide; the plant essential oil and the effective components of the insecticide have good synergistic effect, but have no cross resistance, and can effectively maintain the insecticidal effect of the insecticide.

In this embodiment, the insecticide for the white peltate pest is a mixture of cyhalothrin, spirotetramat and bifenazate.

In this example, the weight percentage of the pesticide synergist in the pesticide composition was 90%.

Example 6

The embodiment discloses a pesticide composition, which comprises a pelecypyriasis alba pest insecticide and the pesticide synergist in the embodiment 3.

The pesticide composition has the following effects and effects: the sweet wormwood oil and the croton oil can dissolve wax secretion outside the body wall of the cycado cyrtonema pekinensis, and the croton oil increases the penetration of the insecticide on the body wall of the cycado cyrtonema pekinensis and improves the insecticidal effect of the insecticide; the plant essential oil and the effective components of the insecticide have good synergistic effect, but have no cross resistance, and can effectively maintain the insecticidal effect of the insecticide.

In this embodiment, the insecticide for pelagic insect is cyhalothrin.

In this example, the weight percentage of the pesticide synergist in the pesticide composition is 80%.

Performance testing

To clearly illustrate the technical effects of the present invention, the following pesticidal agents were carried out.

Description of the materials

A test insect source: female adults of the cycadopsis guineensis (cycadopsis between Zeonella and Hohong Mulberry laboratory in Caesar agricultural engineering institute, Caesar agricultural engineering institute backdoor) were selected from female adults with a white and full mesochite, no holes and no black spots.

Essential oil for testing: citronella oil, cinnamon oil, anise oil, croton oil, and sweet wormwood oil were purchased from Jiangxi Yi Shen botanical flavors GmbH.

Test insecticide: 10% lambda-cyhalothrin was purchased from Shanghai Yue chemical Co., Ltd.

Experimental methods

Experiment 1: the pesticide synergistic agent of the invention has the dissolving effect on the waxy secretions of the cycado beige

The test adopts a dropping method to test the dissolving effect on the wax secretion of the female adult scale. Collecting cycas revoluta lobules with a test object, rejecting unqualified test insects by using an insect needle, and placing the lobules in a culture dish with qualitative filter paper. Diluting the essential oil of the test plant with alcohol to gradient concentration (v: v: essential oil: 95% alcohol: 1:0, 1:1, 1:10, 1:100, 1:1000), sucking 0.1 μ L of the liquid medicine drop on the anterior chest and back plate of the insect body by using an adjustable pipette, continuously observing the dissolving effect of the essential oil on the scale shell, and taking a picture. Each concentration of the drug solution was repeated 10 times, and the control was treated with an equal volume of alcohol.

Experiment 2: the contact toxicity of cinnamon oil to cycado ferri in the pesticide synergistic agent is measured

Cinnamon oil with good dissolution on female adults is selected for activity determination, and an immersion method is adopted in the test. According to the preliminary test, the essential oil to be tested is diluted to 200, 100, 50, 25, 12.5mg/mL with alcohol for standby. Selecting cycads with cycads of cycad. Then gently soaking the leaf of the insect in the medicinal liquid for 5s, taking out, obliquely airing, placing in a culture dish with a diameter of 9cm and a moisture-keeping pad with filter paper, covering, marking, and placing in an incubator with a temperature (26 +/-2) DEG C, a relative humidity of 70%, an illumination intensity of 2000lx and a light-dark ratio of 14 h: 10 h. After 48h, the death condition of the tested insects is investigated, and the insects are killed by using the shrinkage and hardening of the bodies and the overflowing of the tissue-free liquid after the insects are pricked by using the insect needles to open the shield pieces. Repeating the above steps for 3 times, and performing alcohol treatment for preparing medicinal liquid. Calculating mortality, correcting mortality, a virulence regression equation, correlation coefficients and the like.

Experiment 4: 10% high-efficiency cyhalothrin for treating cycadoContact toxicity assay

The pyrethroid was diluted with 0.1% tween 80 in water and alcohol in 5 successive concentration gradients: 200. 100, 50, 25, 12.5. mu.g/mL. Soaking the leaf solution with the tested insects for 5s by using tweezers, taking out, obliquely airing, placing in a culture dish filled with moisturizing filter paper, covering and marking, and placing in an incubator with the temperature (26 +/-2) DEG C, the relative humidity of 70%, the illumination intensity of 2000lx and the light-dark ratio of 14 h: 10 h. After 48h, the death condition of the tested insects is investigated, and the death is judged by using the phenomena that the insect bodies shrink, become hard, and the tissue fluid does not overflow after the insect needles are pricked by picking the shield pieces with the insect needles. Meanwhile, the essential oil with equal concentration and the Tween 80 pure mother liquor are used as a control, and each treatment is repeated three times.

Experiment 5: the pesticide synergistic agent and the cyhalothrin are used together to measure the contact toxicity of the cycado cyrtothrix

Diluting the essential oil with alcohol, preparing a mother liquor with the concentration of 400mg/mL (the diluted concentration of cinnamon oil is 50mg/mL) by using a 0.1% Tween 80 aqueous solution, and then diluting the pyrethrin into 5 continuous concentration gradients by using an auxiliary agent solution: 200. 100, 50, 25, 12.5. mu.g/mL. Soaking the leaf solution with the tested insects for 5s by using tweezers, taking out, obliquely airing, placing in a culture dish filled with moisturizing filter paper, covering and marking, and placing in an incubator with the temperature (26 +/-2) DEG C, the relative humidity of 70%, the illumination intensity of 2000lx and the light-dark ratio of 14 h: 10 h. After 48h, the death condition of the tested insects is investigated, and the death is judged by using the phenomena that the insect bodies shrink, become hard, and the tissue fluid does not overflow after the insect needles are pricked by picking the shield pieces with the insect needles. Meanwhile, the essential oil with equal concentration and the Tween 80 pure mother liquor are used as a control, and each treatment is repeated three times.

Experimental data processing and statistical method

Calculating mortality and corrected mortality of each treatment, performing data analysis statistics with "Excle 2010", finding regression line, LC₅₀And 95% confidence limits.

The mortality rate (%) - (number of test insects-number of live insects after drug)/number of test insects × 100.

Corrected mortality (%) — treatment mortality-control mortality)/(100-control mortality) × 100.

Results and analysis

Analysis of results for experiment 1:

as can be seen from fig. 1 and fig. 2, the citronella oil, the cinnamon oil, the star anise oil, the croton oil and the sweet wormwood oil in the pesticide synergistic agent have different degrees of dissolution on wax secretion of the female adult of the pernicia magna, when the alcohol concentration of the essential oil is 1:1, the cinnamon oil has the strongest solubility, the sweet wormwood oil has the worst effect, and the dissolution performance sequence is as follows: cinnamon oil, croton essential oil, artemisia oil, star anise oil and citronella oil. When the alcohol concentration of the essential oil is 1:10, the cinnamon oil has the strongest solubility, the sweet wormwood oil has the worst effect, and the sequence of the solubility performance is as follows: cinnamon oil, croton essential oil, star anise oil, citronella oil and artemisia oil.

Analysis of the results for experiment 2:

TABLE 1 Activity of cinnamon oil on female Endocarpium ferrugineum adults

The activity of cinnamon oil on female adults of the cycadopsis ferruginea is shown in table 1, and the oral mortality rate of the cycadis is 73.62% when the concentration of the cinnamon oil is 200mg/mL, which indicates that the cinnamon oil has good lethal activity on the cycadis ferruginea. From table 1, the toxicity equation of cinnamon oil to female adult cycado is calculated as Y-3.358 +0.9463x, the correlation coefficient is 0.9741, and the lethal medium concentration LC₅₀It was 54.33. + -. 14 mg/mL. The cinnamon oil can dissolve the wax secretion of the surface of the scale gecko and has good lethal activity. The star anise oil, the citronella oil, the sweet wormwood oil and the croton essential oil have good dissolving effect on wax secretion on the surface layer of the insect body, but have no obvious lethal activity on the cycado.

Analysis of the results for experiment 3 and experiment 4:

TABLE 3 synergistic effect of essential oil in combination with cyhalothrin on female adults of Lecanicillium cyrtomii

Although the synergistic insecticidal effect brought by the citronella oil, the cinnamon oil and the star anise oil as the pesticide synergist is illustrated by taking the citronella oil, the cinnamon oil and the star anise oil as examples, the data of the test croton oil and the sweet wormwood oil are not given here, the croton oil and the sweet wormwood oil are rich in anethole, D-limonene, cinnamaldehyde and methyl salicylate as the croton oil and the sweet wormwood oil are analyzed by a gas chromatography-mass spectrometer, and therefore the croton oil and the sweet wormwood oil are known to have the synergistic effect.

The synergistic effect results of the citronella oil, the cinnamon oil, the star anise oil and the cyhalothrin are shown in table 3, and if the CTC of the compound agent is 80-120 according to a combined toxicity calculation method, the synergistic effect is shown as an additive effect; if the CTC is more than 120, the effect is synergistic; CTC < 80, the antagonism is indicated. The cinnamon oil, the star anise oil and the citronella oil have synergistic effect on the cyhalothrin, wherein the synergistic effect of the cinnamon oil and the star anise oil is obvious, the co-toxicity coefficient is 273.50 and 285.59, the co-toxicity coefficient is obviously higher than that of the citronella oil on the cyhalothrin, and the co-toxicity coefficient is 140.32.

Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention, and not for limiting the protection scope of the present invention, although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.