阅读说明：本技术 适于蛋鸡育雏、育成期阶段使用或肉鸡使用的鸡饲料添加剂组合物和应用 (Chicken feed additive composition suitable for laying hens in brooding and growing periods or broilers and application ) 是由 王友明 邵强 商煜波 何骥远 李奎 徐建宏 孙佰玲 许赟浩 吴吉安 于 2021-09-03 设计创作，主要内容包括：本发明公开了一种适于蛋鸡育雏、育成期阶段使用或肉鸡使用的鸡饲料添加剂组合物,其包含凝结芽孢杆菌、复合中药和α-单月桂酸甘油酯。本发明同时公开了一种含有上述添加剂的鸡饲料。本发明将复合中药、凝结芽孢杆菌和α-GML联用能够通过提高蛋鸡免疫性能,增强肠道屏障功能,促进消化达到促进生长,降低死亡的效果。(The invention discloses a chicken feed additive composition suitable for laying hens in brooding and growing periods or for broilers, which comprises bacillus coagulans, a composite traditional Chinese medicine and alpha-glycerol monolaurate. The invention also discloses a chicken feed containing the additive. The invention combines the compound traditional Chinese medicine, the bacillus coagulans and the alpha-GML together, and can improve the immunity of the laying hens, enhance the intestinal barrier function, promote digestion, and achieve the effects of promoting growth and reducing death.)

1. A chicken feed additive composition suitable for laying hens in brooding and growing periods or for broiler chickens is characterized by comprising bacillus coagulans, a compound traditional Chinese medicine and alpha-glycerol monolaurate.

2. The chicken feed additive composition suitable for laying hens in brooding, growing periods or broiler chickens of claim 1, wherein the mass ratio of the bacillus coagulans to the composite traditional Chinese medicine to the alpha-glycerol monolaurate is 1 (3-10) to (0.5-2).

3. The chicken feed additive composition as claimed in claim 1, wherein the compound Chinese medicine is mainly composed of flos Lonicerae, fructus forsythiae, radix Isatidis, Coptidis rhizoma, Scutellariae radix, fructus Gardeniae, radix Codonopsis, Atractylodis rhizoma, pericarpium Citri Tangerinae, fructus crataegi, rhizoma Dioscoreae, Ginseng radix, radix Ophiopogonis, and radix astragali.

4. The chicken feed additive composition suitable for laying hens in brood, growing periods or broilers of claim 3, wherein the composite traditional Chinese medicine comprises the following components in parts by weight, calculated on 100 parts by weight:

honeysuckle flower: 1-5 parts of

Fructus forsythiae: 1-5 parts of

Radix isatidis: 5-10 parts of

Scutellaria baicalensis: 1-5 parts of

Gardenia: 1-5 parts of

Codonopsis pilosula: 5-15 parts of

White atractylodes rhizome: 1-5 parts of

Dried orange peel: 5-10 parts of

Hawthorn fruit: 1-5 parts of

Chinese yam: 1-5 parts of

Ginseng: 20-35 parts of

Zhe ophiopogon root: 10-25 parts of

Astragalus root: 15-30 parts.

5. The chicken feed additive composition suitable for laying hens in brood, growing period or broiler chicken of claim 1, wherein the Bacillus coagulans is strain WS4-1 with CGMCC NO. 7023.

6. The chicken feed additive composition of claim 1, wherein the bacillus coagulans sample comprises bacillus coagulans 3.14 x 10¹⁰cfu/g and its metabolites, spore formation 85.2%.

7. A chicken feed, which is characterized by comprising the chicken feed additive composition suitable for laying hens in brooding, growing periods or broilers as claimed in any one of claims 1 to 5.

8. The chicken feed according to claim 7, wherein the amount of the chicken feed additive composition added is 0.1-2% of the basic ration.

9. The chicken feed of claim 7, wherein the chicken feed is used in broiler chicken, laying hen brood time and laying hen brood time.

Technical Field

The invention belongs to the technical field of livestock feed research, and particularly relates to a chicken feed additive composition suitable for laying hens in brooding and growing periods or broilers and application thereof.

Background

At present, under the conditions of scale, intensification and high density, laying hens are easily stimulated by various stress factors, and at the early stage of laying hen culture, the laying hens are weak in disease resistance and more susceptible to the threat of various bacterial and viral diseases, so that series of problems such as growth obstruction, immunity inhibition, intestinal dysfunction and the like are caused, and the breeding benefit and success or failure are seriously influenced. The health degree of the laying hens at the initial culture stage has great influence on the subsequent production performance. In the past, antibiotics and some medicines are often relied on in the seedling raising and breeding stages, and the use of the antibiotics and the medicines can harm human health. Therefore, how to develop an antibiotic-free technology in the seedling raising and breeding stages and ensure the smooth breeding is urgent.

The bacillus coagulans is a bacterium which can grow spores and produce lactic acid, has all the functions of promoting intestinal microecological balance, stimulating immunity, keeping organism health, promoting digestion and the like of common probiotics, and has the biological characteristics of strong stress resistance (dryness resistance, gastric acid resistance, easy storage, high temperature and high pressure resistance), field planting and the like. The subject group obtained high-yield and stable bacillus coagulans strains and established an efficient fermentation production process. The viable count of the bacillus is 3.0-4.0 multiplied by 1011cfu/g, and the spore rate is more than 85%.

alpha-GML is a novel feed additive, has multiple functions of emulsifying property, bacteriostasis and virus resistance, regulating lipid metabolism, enhancing immunity, regulating intestinal flora structure and the like, and has no corrosivity, bad smell and taste. The alpha-GML is a surfactant simultaneously having a hydrophilic group and a hydrophobic group, and is added into the feed, so that oil droplets in the feed can be dispersed into fine oil droplets, the contact area of lipase and the fine oil droplets is increased, and the digestion and absorption of fat are promoted. The alpha-GML also has the functions of bacteriostasis and antivirus, and has obvious inhibiting effect on fat envelope viruses such as blue ear disease and the like in livestock and poultry. The alpha-GML can also improve the lipid metabolism of the liver of an animal body and slow down or even treat the aggravation of the fatty liver. The alpha-GML can enhance the immune system of animals and promote the proliferation and differentiation of T lymphocytes. The alpha-GML can also promote the proliferation of beneficial bacteria in the intestinal tract and inhibit the proliferation of harmful bacteria. Is an ideal antibiotic substitute product and has wide development prospect.

Disclosure of Invention

The invention provides a chicken feed additive composition suitable for laying hens in brooding stages, laying hens in growing periods or broilers (including brooding stages and growing periods).

The invention also provides a chicken feed additive containing the composition.

A chicken feed additive composition suitable for laying hens in brooding and growing period and for broiler chickens comprises Bacillus coagulans, compound Chinese medicine and alpha-glycerol monolaurate (alpha-GML).

Preferably, the mass ratio (weight ratio) of the bacillus coagulans, the compound traditional Chinese medicine and the alpha-glycerol monolaurate is 1 (3-10) to 0.5-2. Further preferably 1 (4-6) and (0.8-1.2). Still more preferably 1:5: 1.

Preferably, the compound traditional Chinese medicine mainly comprises honeysuckle, fructus forsythiae, radix isatidis, coptis chinensis, scutellaria baicalensis, gardenia, codonopsis pilosula, bighead atractylodes rhizome, pericarpium citri reticulatae, hawthorn, Chinese yam, ginseng, radix ophiopogonis thunbergii and astragalus mongholicus.

Preferably, the composite traditional Chinese medicine comprises the following components in parts by weight based on 100 parts of the composite medicine:

honeysuckle flower: 1-5 parts of

Fructus forsythiae: 1-5 parts of

Radix isatidis: 5-10 parts of

Scutellaria baicalensis: 1-5 parts of

Gardenia: 1-5 parts of

Codonopsis pilosula: 5-15 parts of

White atractylodes rhizome: 1-5 parts of

Dried orange peel: 5-10 parts of

Hawthorn fruit: 1-5 parts of

Chinese yam: 1-5 parts of

Ginseng: 20-35 parts of

Zhe ophiopogon root: 10-25 parts of

Astragalus root: 15-30 parts.

Preferably, the bacillus coagulans is the strain WS4-1 with the conservation number of CGMCC NO. 7023.

Preferably, the Bacillus coagulans sample contains Bacillus coagulans 3.14X 10¹⁰cfu/g and its metabolites, spore formation 85.2%.

The invention also provides a chicken feed which contains the chicken feed additive composition which is suitable for laying hens in brooding, growing periods or broilers.

Preferably, the addition amount of the chicken feed additive composition is 0.1-2%. Preferably, the addition amount of the chicken feed additive composition is further preferably 0.1 to 0.3%. Specifically, the addition amounts of the bacillus coagulans, the compound traditional Chinese medicine and the alpha-monolaurin are respectively 0.01-0.03%, 0.05-0.2% and 0.01-0.03% of the basic ration.

In the invention, the immunity of the immune system of an animal body is improved by introducing the alpha-GML, and the alpha-GML has an enhancement effect on the immune system for the following reasons: alpha-GML has two-way regulation effect on inflammatory reaction, and lower dosage of alpha-GML has the effect of stimulating the proliferation and differentiation of T cells in the healthy state of animals. T lymphocytes are rapidly proliferated and differentiated to generate mainly by regulating signaling pathways of IL-2, TNF-beta and INF-gamma. The bacillus coagulans can play a role of an immune adjuvant at an antigen recognition part of a digestive tract, so that related lymphoid tissues are in a higher immune monitoring state, thereby accelerating the development of immune organs, the maturation of an immune system, the increase of T lymphocytes and B lymphocytes, the enhancement of phagocytic and secretory functions of mononuclear-macrophages, and the improvement of humoral and cellular immune levels. The Chinese herbal medicine contains various nutrient substances such as protein, amino acid, vitamin, grease, resin, saccharide, plant pigment, macroelements, trace elements and the like, and also contains a large amount of substances such as organic acids, alkaloids, polysaccharide, volatile oil, wax, glycoside, tannin and the like, and unknown growth promoting factors and immune active substances. The combined use of the three shows better effect.

Meanwhile, the alpha-GML has the function of improving the barrier function of intestinal mucosa, can be used as an energy supply substance by intestinal epithelial cells, can promote the growth of the villus epithelial cells and accelerate the replacement of the villus epithelial cells; the alpha-GML can also promote the proliferation of a plurality of intestinal beneficial bacteria, so that the intestinal flora can accelerate the decomposition of carbohydrate to generate short-chain fatty acid, the short-chain fatty acid can be utilized by villus epithelial cells, the expression of the tight junction protein is improved, and the integrity of the intestinal barrier is maintained. And produces a complementary effect in bacillus coagulans.

Test results show that the survival rate of laying hens in brooding period and growing period can be remarkably improved and the death rate can be remarkably reduced by adding the compound traditional Chinese medicine, the bacillus coagulans and the alpha-GML. The analysis result can predict that the composition consisting of the compound traditional Chinese medicine, the bacillus coagulans and the alpha-GML can promote the repair or maintain the integrity of the intestinal tract tissue structure of the chicken, can efficiently regulate and control the balance of the intestinal flora of the livestock and poultry, and can reduce the toxic and side effects of harmful bacteria and reduce the death.

Experiments show that the feed additive provided by the invention has the advantages that IgM, IL-2 and IFN-gamma test groups in the immunity indexes of 16-week-old laying hens are respectively improved by 13.65% (P is less than 0.05), 44.87% (P is less than 0.05) and 82.03% (P is less than 0.05), and the difference is very obvious.

The test result of the influence of the combination of the compound traditional Chinese medicine, the bacillus coagulans and the alpha-GML on the intestinal barrier of the Jingbai No. 1 laying hen shows that aiming at the indexes of AKP and sIgA of 16-week-old laying hens, the test group respectively improves 10.38 percent (P is less than 0.05) and 11.62 percent (P is less than 0.05), and the difference is very obvious.

In conclusion, the compound traditional Chinese medicine, the bacillus coagulans and the alpha-GML are combined, so that the effects of improving the immunity of the laying hens, enhancing the intestinal barrier function and promoting digestion to promote growth and reduce death can be achieved.

Detailed Description

The invention is further illustrated by the following examples:

taking out raw materials: bacillus coagulans (strain WS4-1 obtains China general microbiological culture Collection centerThe strain preservation number of CGMCC No.7023) is determined by plant protection and microorganism research institute of agricultural academy of sciences of Zhejiang province, and the batch of the product (batch number: 2019031203) contains Bacillus coagulans 3.14X 10¹⁰cfu/g and its metabolites, spore formation 85.2%.

alpha-GML was produced and supplied by daily use auxiliary of Shandong Shanghai, Jiaxing, lot No. 20190113 as a white powder. It is obtained by directly carrying out esterification reaction on lauric acid and glycerol under the action of a catalyst. The content of alpha-GML of the batch is 91.5 percent according to the self test of the company.

The traditional Chinese medicine is prepared by scientifically combining and processing heat-clearing and detoxifying medicines and spleen-tonifying and appetite-promoting medicines, and mainly comprises honeysuckle, fructus forsythiae, isatis root, coptis root, scutellaria baicalensis, gardenia, codonopsis pilosula, bighead atractylodes rhizome, dried orange peel, hawthorn, Chinese yam, ginseng, thunberg lilyturf root and astragalus (the medicines are purchased in the market and provided by Honglide pharmaceutical industry limited company of the Bo of Anhui).

The concrete weight portions are as follows:

honeysuckle flower: 2 parts of (1);

fructus forsythiae: 3 parts of a mixture;

radix isatidis: 7 parts;

scutellaria baicalensis: 2 parts of (1);

gardenia: 3 parts of a mixture;

codonopsis pilosula: 9 parts of (1);

white atractylodes rhizome: 2 parts of (1);

dried orange peel: 8 parts of a mixture;

hawthorn fruit: 3 parts of a mixture;

chinese yam: 2 parts of (1);

ginseng: 24 parts of (1);

zhe ophiopogon root: 15 parts of (1);

astragalus root: and 20 parts.

And (3) test period: 4/month 1 to 8/month 4 in 2019, with a period of 16 weeks.

Brooding stage 0-8 weeks, and breeding stage 9-16 weeks.

A place: Cixi-Daaiji corporation Cixi-Xinpu laying hen farm 1# brooding house

Animal selection and design of experiments

1. 2000 1 day old, Jingbai No. 1 laying hens were selected and randomly divided into 2 treatment groups of 5 replicates each, with 200 replicates each. The control group was fed with basal diet (no antibiotic feed, supplemented with bacillus subtilis), the test group was fed with bacillus coagulans, traditional Chinese medicine and GML supplemented with basal diet, and the specific test groups are shown in the following table:

table 1 experimental group design

2. Basal diet composition and nutritional level

The basic diet is prepared according to the nutritional requirements of NRC (1994) laying hens and by combining production practice, and the weight composition and the nutritional level of the basic diet are shown in a table 2.

TABLE 2 basic diet composition and nutritional levels (air-dried basis)

The premix is provided by Zhejiang university. In the table, "egg + cystine" means methionine + cystine.

3. Feeding management

A ground free-ranging mode is adopted, during the test period, a specially-assigned person is responsible for raising, natural ventilation and free drinking are realized, the hurdle is kept clean and sanitary, and the immunization program is carried out according to the conventional method.

4. Slaughter and sample collection

One day before the test of 16 weeks, 12 chicken carotid arteries in each group were randomly bled and serum was naturally separated out, centrifuged and subpackaged.

Second, collecting experimental data and analyzing experimental results

The experimental data were subjected to preliminary statistical analyses using Excel, followed by analysis of variance using SPSS (16.0) statistical software, and multiple comparisons using least significant difference method (LSD). P <0.05 was used as a criterion for significance of difference, and the results were expressed as mean ± standard deviation (X ± SD).

1. Determination of influence of combination of compound traditional Chinese medicine, bacillus coagulans and alpha-GML on growth performance of Jingbai No. 1 laying hen

The determination method comprises the following steps: each repetition is a statistical unit, and the weight and death condition of each repeated laying hen are regularly recorded every day during the test period. The results are shown in Table 3:

TABLE 3 influence of the combination of Chinese medicinal composition, Bacillus coagulans and alpha-GML on the growth performance of Jingbai No. 1 laying hen

Note: the data in the same row indicate that the difference is obvious when the lower case letters are marked on the shoulder marks (P is less than 0.05), and the difference is not obvious when no letters or the same letters indicate that the difference is not obvious (P is more than or equal to 0.05). The same applies below.

As can be seen from Table 3: at the end of the age of 8 weeks and 16 weeks, the body weight of the test group was increased by 7.98% (P < 0.05) and 5.40% (P < 0.05) respectively compared with the control group. The daily weight gain of the test group is respectively increased by 8.75 percent (P is less than 0.05), 3.69 and 5.53(P is less than 0.05) compared with the control group at 0-8 weeks, 9-16 weeks and 0-16 weeks, and the test group is slightly increased but has no significant difference (P is more than 0.05) compared with the control group at 9-16 weeks. The mortality rates of the test group and the control group are respectively reduced by 30.56% (P < 0.05), 42.86(P < 0.05) and 35.09% (P < 0.05) in 0-8 weeks, 9-16 weeks and 0-16 weeks.

The test result shows that the addition of the compound traditional Chinese medicine, the bacillus coagulans and the alpha-GML can obviously improve the survival rate of the Jingbai No. 1 laying hen in brooding period and breeding period and obviously reduce the death rate. The analysis result can predict that the composition consisting of the compound traditional Chinese medicine, the bacillus coagulans and the alpha-GML can promote the repair or maintain the integrity of the intestinal tract tissue structure of the chicken, can efficiently regulate and control the balance of the intestinal flora of the livestock and poultry, and can reduce the toxic and side effects of harmful bacteria and reduce the death.

2. Influence of combination of compound traditional Chinese medicine, bacillus coagulans and alpha-GML on serum immunity index of Jingbai No. 1 laying hen

The detection method comprises the following steps: the measurement of chicken immunoglobulin A (IgA), chicken immunoglobulin G (IgG), and chicken immunoglobulin M (IgM) in serum was performed at the second national hospital in Hangzhou using a fully automated biochemical analyzer (BECKMAN COULTER, model IMMAGE 800). Chicken interferon-gamma (IFN-gamma), interleukin 2(IL-2) and interleukin 4(IL-4) in serum are all measured by a kit method, the kit is purchased from Nanjing to build a biotechnology company Limited, and the preparation of reagents and related experimental operations are carried out according to the steps of a specification.

The main apparatus is as follows: a multifunctional continuous spectrum microplate reader model SpectraMax M5, Molecular Devices, usa; high-speed refrigerated centrifuge, model 5417R, Eppendorf, Germany, etc.

The results are shown in Table 4:

TABLE 4 influence of combination of Chinese medicinal composition, Bacillus coagulans and alpha-GML on serum immunity index of Jingbai No. 1 laying hen

As can be seen from Table 4: in the immunity indexes of 16-week-old laying hens, IgM, IL-2 and IFN-gamma test groups are respectively improved by 13.65% (P is less than 0.05), 44.87% (P is less than 0.05) and 82.03% (P is less than 0.05) compared with a control group, and the difference is very obvious.

3. Influence of combination of compound traditional Chinese medicine, bacillus coagulans and alpha-GML on intestinal barrier of Jingbai No. 1 laying hen

The detection method comprises the following steps: the measured intestinal tract barrier digestion function indexes are alkaline phosphatase (AKP) and diamine oxidase (DAO) in serum. The intestinal barrier immune function index is secretory immunoglobulin A (sIgA). The determination is carried out by adopting a kit method, the kit is purchased from Nanjing to build a biotechnology limited company, and the preparation of the reagent and the experimental operation are carried out according to the steps of the instruction.

The main apparatus is as follows: a multifunctional continuous spectrum microplate reader model SpectraMax M5, Molecular Devices, usa; ultraviolet spectrophotometer, model UV-2000, pharmacia, Sweden; tissue grinders, model T165-48, shanghai jing xin test science and technology ltd; high-speed refrigerated centrifuge, model 5417R, Eppendorf, Germany.

The results are shown in Table 5:

TABLE 5 influence of combination of Chinese medicinal composition, Bacillus coagulans and alpha-GML on intestinal barrier of Jingbai No. 1 laying hen

As can be seen from Table 4: compared with a control group, indexes of AKP and sIgA of 16-week-old laying hens are respectively improved by 10.38% (P is less than 0.05) and 11.62% (P is less than 0.05), and the difference is very obvious.

According to the analysis, the combination of the compound traditional Chinese medicine, the bacillus coagulans and the alpha-GML can improve the immunity of the laying hens, enhance the intestinal barrier function and promote digestion to achieve the effects of promoting growth and reducing death.