阅读说明：本技术 一种具有祛眼袋功效的眼霜组合物及其制备方法 (Eye cream composition with pouch removing effect and preparation method thereof ) 是由 杨帆 周紫燕 祝希宇 郭苗 周正 郭阳 于 2021-09-26 设计创作，主要内容包括：本发明公开了一种具有祛眼袋功效的眼霜组合物及其制备方法。该具有祛眼袋功效的眼霜组合物包含的原料组分有：1.0-35.0％的眼部祛眼袋功效成分、5.0-40.0％的润肤剂、0.5-30.0％的乳化剂、0.5-20.0％的填充剂、0.1-10.0％的增稠剂、0.3-5.0％的防腐剂、0.01-1.0％的日用香精及去离子水余量。所述眼部祛眼袋功效成分是由长心卡帕藻提取物、十四烷基氨基丁酰缬氨酰胺基丁酸脲三氟乙酸盐、二肽二氨基丁酰苄基酰胺二乙酸盐和乙酰基四肽-5组成。本发明的祛眼袋功效成分从诱发眼袋的眼部皮肤松弛和水肿因素入手,能有效减缓可见的眼部眼袋突出问题,以带来更具魅力的面貌。(The invention discloses an eye cream composition with an eye pouch removing effect and a preparation method thereof. The eye cream composition with the effect of removing the pouch comprises the following raw material components: 1.0-35.0 percent of eye pouch-removing effective component, 5.0-40.0 percent of emollient, 0.5-30.0 percent of emulsifier, 0.5-20.0 percent of filler, 0.1-10.0 percent of thickener, 0.3-5.0 percent of preservative, 0.01-1.0 percent of daily essence and the balance of deionized water. The eye pouch-removing effective component comprises Kappaphycus alvarezii extract, tetradecyl aminobutyryl valinamide butyric urea trifluoroacetate, dipeptide diaminobutyryl benzylamide diacetate and acetyl tetrapeptide-5. The pouch-removing effective component provided by the invention starts with factors of pouch-inducing eye skin relaxation and edema, and can effectively alleviate visible eye pouch protrusion problems, so as to bring more attractive appearance.)

1. An eye cream composition with an effect of removing pouches is characterized in that: based on the total weight of the composition, the composition consists of the following raw materials:

1.0-35.0% of an eye pouch-removing effective component, wherein the eye pouch-removing effective component comprises: kappaphycus alvarezii extract 0.1-30%, tetradecylaminobutylamidobutanamide urea trifluoroacetate 0.1-30%, dipeptide diaminobutyrylbenzylamide diacetate 0.1-30%, and acetyl tetrapeptide-50.1-30%;

5.0-40.0% of an emollient;

0.5-30.0% of emulsifier;

0.5-20.0% of a filler;

0.1-10.0% of a thickening agent;

0.3-5.0% of preservative;

0.01-1.0% of daily essence and the balance of deionized water.

2. The eye cream composition with the pouch removing effect as claimed in claim 1, wherein: the composition comprises the following components in percentage by weight based on the total weight of the composition:

1.0-25.0% of an eye pouch-removing effective component, wherein the eye pouch-removing effective component comprises: kappaphycus alvarezii extract 0.5-20%, tetradecylaminobutylamidobutanamide urea trifluoroacetate 0.5-20%, dipeptide diaminobutyrylbenzylamide diacetate 0.5-20%, and acetyl tetrapeptide-50.5-20%;

3. the eye cream composition with the pouch removing effect as claimed in claim 1, wherein: the eye pouch-removing effective components comprise:

1-10% of Kappaphycus alvarezii extract, 1-10% of tetradecyl amino butyryl valinamide butyrate trifluoroacetate, 1-10% of dipeptide diaminobutyryl benzylamide diacetate and 51-10% of acetyl tetrapeptide.

4. The eye cream composition with the pouch removing effect as claimed in claim 1, wherein: the eye pouch-removing effective components comprise:

kappaphycus alvarezii extract 0.5-5%, tetradecylaminobutylamidobutanamide urea trifluoroacetate 0.5-5%, dipeptide diaminobutyrylbenzylamide diacetate 0.5-5%, and acetyl tetrapeptide-50.5-5%.

5. The eye cream composition with the pouch removing effect as claimed in claim 1, wherein:

the emollient is at least one selected from caprylic capric triglyceride, squalane, avocado oil, shea butter, jojoba oil, glycerol, butanediol, hexanediol, 1, 2-pentanediol, panthenol, sodium hyaluronate, tocopherol acetate, polydimethylsiloxane, dimethiconol, PEG-11 methyl ether dimethicone, C12-16 alcohol, phytosterol/octyldodecyl lauroyl glutamate, hydrogenated polyisobutene, rapeseed oil, polydecene, soybean oil, olive oil, isododecane, isohexadecane, stearyl alcohol and behenyl alcohol;

the emulsifier is selected from at least one of olive oil, palmitic acid, cholesterol, PEG-7 esters, PEG-8 caprylic/capric glycerides, sorbitan olive oleate, polysorbate-20, octyldodecanol, laureth-23, stearic acid, hydrogenated lecithin, oleyl erucate, steareth-21, palmitol and PEG-75 stearate and mixtures of glyceryl stearate and steareth-20, polysorbate-80, soya lecithin PC60, mixtures of glyceryl stearate and PEG-75 stearate, poloxamer 407, PEG-30 dipolyhydroxystearate, ceteareth-25;

the filler is selected from at least one of titanium dioxide, mica, boron nitride, polymethylsilsesquioxane, diatomite, kaolin, cellulose acetate, aluminum octyl succinate starch, talcum powder, zinc sulfate and volcanic ash;

the thickening agent is selected from at least one of hydroxyethyl cellulose, 1, 3-propylene glycol, xanthan gum, carbomer, acrylic acid (ester)/copolymer, polymer EMT-10, polyacrylate cross-linked polymer-6, sodium acrylate/sodium acryloyldimethyl taurate copolymer, acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, acryloyldimethyl taurate/VP copolymer, polyethylene and gum arabic;

the antiseptic is at least one selected from phenoxyethanol, sodium benzoate, triclosan and chlorphenesin.

6. The preparation method of the eye cream composition with the effect of removing the bags under the eyes as claimed in any one of claims 1 to 5, which is characterized by comprising the following process steps:

1) wetting and completely dispersing the thickening agent in water to obtain a mixed solution;

2) adding eye pouch removing functional components, an emollient, a filler and a preservative into the mixed solution obtained in the step 1), heating to 65 ℃, and homogenizing at 3000r/min for 3 minutes to completely disperse the eye pouch removing functional components;

3) adding an emulsifier and a daily essence into the step 2), homogenizing at 3000r/min for 3 minutes to completely emulsify the mixture, and heating to 65 ℃ to obtain the eye cream composition with the effect of removing the pouches in the eyes.

7. A cosmetic characterized by: the cosmetic comprises the eye cream composition with the function of removing the pouch, which is prepared by the preparation method of claim 6.

8. The cosmetic of claim 7, wherein: the cosmetic is in the form of a solvent dispersion or suspension, or in the form of emulsified particles, or in the form of a polymer coating.

Technical Field

The invention relates to a cosmetic composition and a preparation method thereof, belongs to the technical field of daily chemicals, and particularly relates to an eye pouch removing eye cream composition containing various eye pouch removing functional components and a preparation method thereof.

Background

Under-eye bags are a skin problem that seriously affects the beauty of the face and are also a sign that predicts aging. The occurrence of bags under the eyes is mainly due to two factors: on the one hand, the eye skin loses elasticity with age, the eye muscles are also relaxed, wrinkles are formed on the eyelid, and fat lining the eye orbit is transferred from the eye cavity and accumulated on the eyelid to form eye bags. Yet another important reason is that the lymphatic circulation is weakened and the permeability of the capillaries is increased, resulting in edema and thus formation of pouches. In life, the people have irregular work and rest habits, and often stay up all night, so that the sleep is insufficient, and long-term fatigue causes unsmooth eye microcirculation and pouch generation. In addition, the lack of water in the eye skin is also strongly related to the appearance of loose pouches. Under-eye bags are a difficult cosmetic technique to mask the face, severely affecting the individual's mental appearance.

However, the skin care products for removing the pouches in the current market often have various defects of unobvious pouch removing effect, long effective period, high sensitization and the like. In the current research, although some components are applied to cosmetics singly or pairwise, the combination of a plurality of components with the pouch removing effect is not applied to eye cream, the pouch removing effect is exerted synergistically, and the effects of fading black eye circles on eye lines and the like are achieved.

Accordingly, there is an increasing need for non-invasive, effective and risk-free cosmetic formulations that can reduce and minimize the appearance of ocular skin defects, thereby providing a perpetual youthful and vibrant appearance. How to provide an eye cream skin care product with better pouch removing performance is a technical problem which needs to be solved urgently by technical personnel in the field at present.

Disclosure of Invention

In order to solve the problems in the prior art, the invention aims to provide an eye pouch-removing eye cream composition which contains multiple functional components and can synergistically increase the pouch-removing effect.

The invention also aims to provide a preparation method of the eye cream composition with the function of removing the bags under the eyes.

The technical scheme adopted by the invention is as follows.

The eye cream composition with the effect of removing the bags under the eyes comprises the following raw material components in percentage by weight:

1.0-35.0% of an eye pouch-removing effective component, which comprises: kappaphycus alvarezii extract 0.1-30%, tetradecylaminobutylamidobutanamide urea trifluoroacetate 0.1-30%, dipeptide diaminobutyrylbenzylamide diacetate 0.1-30%, and acetyl tetrapeptide-50.1-30%;

5.0-40.0% of an emollient;

0.5-30.0% of emulsifier;

0.5-20.0% of a filler;

0.1-10.0% of a thickening agent;

0.3-5.0% of preservative;

0.01-1.0% of daily essence;

the balance of deionized water.

Further, the eye cream composition with the function of removing the pouch on the eyes comprises the following components in parts by weight:

1.0-25.0% of an eye pouch-removing effective component, which comprises: kappaphycus alvarezii extract 0.5-20%, tetradecylaminobutylamidobutanamide urea trifluoroacetate 0.5-20%, dipeptide diaminobutyrylbenzylamide diacetate 5-20%, and acetyl tetrapeptide-50.5-20%;

further, the eye pouch removing effective component comprises the following raw material components:

kappaphycus alvarezii extract 0.5-10%, tetradecylaminobutylamidobutanamide urea trifluoroacetate 0.5-10%, dipeptide diaminobutyrylbenzylamide diacetate 0.5-10%, and acetyl tetrapeptide-50.5-10%.

Further, the eye pouch removing effective component comprises the following raw material components:

kappaphycus alvarezii extract 0.5-5%, tetradecylaminobutylamidobutanamide urea trifluoroacetate 0.5-5%, dipeptide diaminobutyrylbenzylamide diacetate 0.5-5%, and acetyl tetrapeptide-50.5-5%.

Further, the eye pouch removing effective component comprises the following raw material components:

kappaphycus alvarezii extract 0.5-3%, tetradecylaminobutylamidobutanamide urea trifluoroacetate 0.5-3%, dipeptide diaminobutyrylbenzylamide diacetate 0.5-3%, and acetyl tetrapeptide-50.5-3%.

Further, the eye cream composition with the pouch removing effect on the eyes comprises the following components in parts by weight:

2.5-9.0% of an eye pouch-removing effective component, which comprises: 1-5% of Kappaphycus alvarezii extract, 0.5-1.0% of tetradecyl amino butyryl valinamide butyrate trifluoroacetate, 0.5-1.0% of dipeptide diaminobutyryl benzylamide diacetate and 50.5-1.5% of acetyl tetrapeptide;

further, the emollient is selected from at least one of caprylic capric triglyceride, squalane, avocado oil, shea butter, jojoba oil, glycerin, butylene glycol, hexylene glycol, 1, 2-pentanediol, panthenol, sodium hyaluronate, tocopherol acetate, polydimethylsiloxane, dimethiconol, PEG-11 methyl ether dimethicone, C12-16 alcohol, phytosterol/octyldodecyl lauroyl glutamate, hydrogenated polyisobutene, rapeseed oil, polydecene, soybean oil, olive oil, isododecane, isohexadecane, stearyl alcohol, and behenyl alcohol. The content thereof is selected from the range of 5-30%, preferably the range of 8-15%.

The emulsifier is selected from at least one of olive oil, palmitic acid, cholesterol, PEG-7 esters, PEG-8 caprylic/capric glycerides, sorbitan olive oleate, polysorbate-20, octyldodecanol, laureth-23, stearic acid, hydrogenated lecithin, oleyl erucate, steareth-21, palmitol and PEG-75 stearate and mixtures of glyceryl stearate and steareth-20, polysorbate-80, soya lecithin PC60, mixtures of glyceryl stearate and PEG-75 stearate, poloxamer 407, PEG-30 dipolyhydroxystearate, ceteareth-25. The content is selected from the range of 0.5-15%, preferably 1-10%.

The filler is at least one selected from titanium dioxide, mica, boron nitride, polymethylsilsesquioxane, diatomite, kaolin, cellulose acetate, aluminum starch octylsuccinate, talcum powder, zinc sulfate and volcanic ash. The content is selected from the range of 0.2-20%, preferably from the range of 0.5-15%, and most preferably from the range of 1% -4%.

The thickening agent is at least one selected from hydroxyethyl cellulose, 1, 3-propylene glycol, xanthan gum, carbomer, acrylic acid (ester)/copolymer, polymer EMT-10, polyacrylate cross-linked polymer-6, sodium acrylate/sodium acryloyldimethyl taurate copolymer, acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, acryloyldimethyl taurate/VP copolymer, polyethylene and arabic gum. The content is in the range of 0.5-8%, preferably in the range of 1-5%.

The antiseptic is at least one selected from phenoxyethanol, sodium benzoate, triclosan and chlorphenesin.

In addition, the invention also discloses a preparation method of the eye cream composition with the effect of removing the pouch of the eyes, which comprises the following process steps:

1) wetting and completely dispersing the thickening agent in water to obtain a mixed solution;

2) adding eye pouch removing functional components, an emollient, a filler and a preservative into the mixed solution obtained in the step 1), heating to 65 ℃, and homogenizing at 3000r/min for 3 minutes to completely disperse the mixed solution composition;

3) adding an emulsifier and a daily essence into the mixed liquid composition formed in the step 2), homogenizing at 3000r/min for 3 minutes to completely emulsify the mixture, and heating to 65 ℃ to obtain the eye cream composition with the eye pouch removing effect on eyes.

Specifically, the eye cream composition with the pouch removing effect for the eyes has a pH value within a range of 4-10, preferably a pH value within a range of 5-8, and most preferably a pH value within a range of 5-7.

Specifically, the eye cream composition with the pouch removing effect for the eyes can be in a solvent dispersion or suspension mode, can also be in the form of emulsified particles or polymer wrapping mode.

Specifically, the cosmetic of the eye pouch-removing effect composition is a skin care product, in particular emulsion, essence, cream, eye cream, body lotion, gel and sun block.

By means of the technical scheme, the invention has the following advantages and beneficial technical effects:

1) the invention contains various effective components with the effects of reducing swelling and improving skin relaxation, such as kappaphycus alvarezii extract, myristylamino butyryl valinamide butyrate trifluoroacetate, dipeptide diaminobutyryl benzylamide diacetate, acetyl tetrapeptide-5 and the like, and can play a role in reducing eye bags on eye skin.

2) The eye pouch-removing efficacy component composition is selected from Kappaphycus alvarezii extract, tetradecyl amino butyryl valinamide butyric urea trifluoroacetate, dipeptide diaminobutyryl benzylamide diacetate and acetyl tetrapeptide-5, and cosmetics can effectively remove eye pouches through preferable combination and efficacy test, and the most preferable composition proportion is found.

3) Compared with most of cosmetics sold on the market, the pouch removing eye cream composition provided by the embodiment of the invention has a remarkable synergistic effect, is suitable for more people with different skin types by using the eye cream, is wide in application range, and is obviously reduced in pouch and obviously improved in skin elasticity after being used by a user. The components for relieving swelling and removing pouch of the eye of the invention cooperate with each other to achieve the efficacy of relieving swelling and removing pouch safely and effectively.

4) The eye pouch removing eye cream composition can reduce lower eyelid edema, provides a plurality of ideal benefits for eye care, and brings younger face.

Drawings

FIG. 1 is a schematic view showing reinforced collagen fibers of a pouch-removing composition;

FIG. 2 is a graph showing the expression of reinforced proteoglycan of the pouch-removing effect composition;

FIG. 3 is a graph showing a comparison of the before and after pouch volume values using the eye cream composition of example 1;

FIG. 4 shows relative change in pouch volume before and after use of the eye cream composition of example 1;

fig. 5 shows the rate of change of pouch volume before and after using the eye cream composition of example 1.

Detailed Description

The invention discloses an eye cream composition with an eye pouch removing effect and a preparation method thereof. The eye pouch-removing cream composition comprises the following raw material components:

1.0-35.0 percent of eye pouch-removing effective component, 5.0-40.0 percent of emollient, 0.5-30.0 percent of emulsifier, 0.5-20.0 percent of filler, 0.1-10.0 percent of thickener, 0.3-5.0 percent of preservative, 0.01-1.0 percent of daily essence and the balance of deionized water.

The eye pouch-removing effective component is prepared by mixing Kappaphycus alvarezii extract, tetradecyl amino butyryl valinamide butyric urea trifluoroacetate, dipeptide diaminobutyryl benzylamide diacetate and acetyl tetrapeptide-5.

Kappaphycus alvarezii is a red algae growing in tropical or subtropical regions, such as Hainan, Xisha Islands, Taiwan and the like. The extract contains carrageenan as main ingredient, and also contains abundant amino acids (such as glycine, leucine, glutamic acid, etc.) and small amount of polyphenols. Therefore, the Kappaphycus alvarezii extract has better hygroscopicity, improves the stratum corneum structural integrity of dry skin and the biosynthesis function of epidermis, provides sufficient water and nutrient minerals for the skin, is beneficial to keeping the water level of the skin all day long, regulates the dynamic balance of the skin and improves the phenomenon of water deficiency and relaxation of the skin.

The preparation method of the Kappaphycus alvarezii extract comprises the following steps:

1) selecting algae: selecting fresh Kappaphycus alvarezii, removing impurities such as straw sticks, silt and the like attached to the leaves of the Kappaphycus alvarezii, soaking in a 75% ethanol solution for 15-30 min, and airing for later use;

2) drying and powdering: drying the kappaphycus alvarezii pretreated in the step 1) in an environment of 50-80 ℃ for 36-48 hours until the water content in the algae leaves is reduced to below 8%, taking out the dried kappaphyalvarezii alvarezii, powdering, and sieving with a sieve of 80-200 meshes to obtain algae powder;

3) foaming and ultrasonic treatment: mixing and stirring the algae powder in the step 2) with water according to a mass ratio of 1:20, soaking for 8 hours to obtain a mixed solution, placing the mixed solution in an ultrasonic cell crusher, and carrying out ultrasonic treatment for 6-8 hours under the condition of 200-500W of power to obtain an ultraphonic solution of kappaphycus alvarezii;

4) and (3) enzymolysis and fermentation: adding citric acid into the algae ultrasonic solution obtained in the step 3), adjusting the pH value of the solution to 4.0-6.0, and adding 6 multiplied by 10 into the algae ultrasonic solution⁴～8×10⁴U/L pectase and 0.1X 10⁴～1×10⁴Carrying out enzymolysis on U/L cellulase for 3-5 hours at the temperature of 40-60 ℃; heating the algae ultrasonic solution subjected to enzymolysis to boiling, inactivating enzymes for 5-20 minutes, and cooling to room temperature to obtain an algae enzymolysis solution;

5) filtering, distilling and collecting under reduced pressure: adding 90% ethanol into the algae enzymolysis liquid in the step 4), extracting for 12h at 30 ℃, filtering, taking the filtrate, distilling under reduced pressure, and removing the ethanol in the solution to obtain the Kappaphycus alvarezii extract.

The tetradecyl amino butyryl valinamide butyrate urea trifluoroacetate can promote the generation of basement membrane glycan and decorin glycan of a dermis layer, endogenously promote the generation of epidermal and dermal hyaluronic acid, help skin absorb water from the body and the skin surface layer, also can enhance the long-time water retention capacity of the skin, can improve the quality of collagen fibers after long-term use, and has the effects of reshaping and firming the skin. Can act on the dermis layer, and can improve the skin relaxation phenomenon by promoting the synthesis of hyaluronic acid of natural skin and increasing the synthesis of collagen fiber.

Dipeptide diaminobutyrylbenzylamide diacetate is a small peptide that mimics the activity of the snake venom toxin Waglerin I, acts on the postsynaptic membrane, and is a reversible antagonist of the muscle nicotinic acetylcholine receptor (nmAChR). Dipeptide diaminobutyrylbenzylamide diacetate binds to the epsilon subunit of nmAChR, blocking the binding of acetylcholine to the receptor, eventually leading to receptor blockade, in which sodium ions cannot be taken up, depolarization cannot occur, neuronal excitation transmission is blocked, and muscle is thus relaxed. Therefore, the skin can be squeezed by the muscle, and the loose state of the skin around the eyes can be relieved.

Acetyl tetrapeptide-5 has two routes of pouch removal, the first being the inhibition of glycosylation of the skin around the eye. Glycosylation often results in abnormal, chaotic cross-linking of collagen fibers. Rather than allowing all collagen fibers to work synergistically in a uniform, organized manner, glycation results in a reduction in the elasticity of the skin. Acetyl tetrapeptide-5 can increase vascular permeability and effectively eliminate edema. Acetyl tetrapeptide-5 counteracts this situation by binding to one of the proteins involved in glycosylation, preventing that protein from binding to the next substance required for continued glycosylation, thereby stopping glycosylation on its trajectory. The second approach is to reduce the amount of fluid that accumulates in the area by altering the permeability of the blood vessels in the area, and by reducing the permeability of the blood vessels in the area.

The composition with the effect of removing the bags in the eyes consists of kappaphycus alvarezii extract, tetradecyl amino butyryl valinamide butyrate trifluoroacetate, dipeptide diaminobutyryl benzylamide diacetate and acetyl tetrapeptide-5. The Kappaphycus alvarezii extract in the composition provides sufficient moisture and nutrient minerals for skin, maintains skin elasticity, ensures skin smoothness and elasticity, retains moisture and water, nourishes skin, and prevents skin from dryness and laxity. The acetyl tetrapeptide-5 can diminish inflammation and eliminate eye edema by changing vascular permeability, and then the tetradecyl amino butyryl valinamide butyric urea trifluoroacetate, the dipeptide diaminobutyryl benzyl amide diacetate and the acetyl tetrapeptide-5 exert the effects of tightening skin and improving skin elasticity, and the eye pouch dispelling effect is improved by the synergistic effect, and meanwhile, the eye fine wrinkles and melanin deposition fading effect are achieved.

The pouch-removing effective component starts from factors of pouch-inducing eye skin relaxation and edema, and can effectively alleviate visible eye pouch protrusion problem, so as to bring more attractive appearance.

The present invention is illustrated by the following examples, which are provided for the purpose of further illustration only and are not intended to limit the scope of the present invention. Other insubstantial modifications and adaptations of the present invention can be made without departing from the scope of the present invention.

Unless otherwise specified, the following percentages (%) are mass percentages. All the raw material components of the invention are commercial products.

Examples 1 to 4

An eye cream composition with an eye pouch removing effect is prepared into eye pouch removing eye cream, and the content of the raw material components is shown in the following table 1.

Table 1 raw material component ratios (% by mass) of examples 1 to 4

Comparative examples 1 to 8

An eye cream composition with an eye pouch removing effect is prepared into eye pouch removing eye cream, wherein the content of the raw material components in comparative examples 1-8 is shown in the following table 2.

TABLE 2 raw material composition ratio of comparative examples 1 to 8 (% by mass)

The preparation method of the eye cream composition with the effect of removing the bags under the eyes comprises the following process steps:

1) wetting and completely dispersing the thickening agent in water to obtain a mixed solution;

2) adding eye pouch removing functional components, an emollient, a filler and a preservative into the mixed solution obtained in the step 1), heating to 65 ℃, and homogenizing at 3000r/min for 3 minutes to completely disperse the eye pouch removing functional components;

3) adding an emulsifier and a daily essence into the step 2), homogenizing at 3000r/min for 3 minutes to completely emulsify the mixture, and heating to 65 ℃ to obtain the eye cream composition with the effect of removing the pouches in the eyes.

The pH values of examples 1 to 4 were 6.0 to 7.0, respectively.

Test example for Effect verification

Effect raw Material-enhanced proteoglycan expression Effect test example 1

1) Test samples: the composition with the effect of removing the bags under the eyes in the embodiment 1 comprises kappaphycus alvarezii extract, tetradecyl aminobutyroylvalinamide butyric urea trifluoroacetate, dipeptide diaminobutyroylbenzylamide diacetate and acetyl tetrapeptide-5; two functional components are formed; the specific composition is shown in table 3.

TABLE 3 efficacy raw material fortification proteoglycan expression effect test component ratio (unit: concentration. mu.M)

2) The test method comprises the following steps: tested on Normal Human Fibroblasts (NHF). Cells were cultured for three days in the absence of calf serum (FCS) and expression of decorin and basement membrane glycans was measured.

3) And (3) testing results:

the mechanism of strengthening collagen fibers by the pouch-removing effect composition is shown in figure 1, and decorin and basilemonase are two important proteoglycans in the dermis. Their protein fraction will bind to the collagen fibers, while the glycosaminoglycan fraction will separate and fill the spaces between the collagen fibers. Before the functional composition is added, the collagen fiber pores of the dermis layer of the skin are uneven, and the expression quantity of decorin and basement membrane polysaccharide with filling function is limited; after the pouch removing composition is added, the levels of decorin and basilemannan are obviously improved, the connection of collagen fibers is enhanced, fine lines caused by fiber breakage are reduced, gaps among collagen fibers are filled with glycosaminoglycan, the pore diameter of the fibers is recovered to be normal, the collagen fibers are healthier, and the skin is recovered to be in a young state.

Experimental results as shown in fig. 2, the effect of the efficacy composition of synergistic example 1 on the expression of both proteoglycans was greater than that of the efficacy composition of both efficacy. Specifically, the efficacy composition increases the expression level of basement membrane polysaccharides by 70%, increases the expression level of decorin by 77%, and the expression level of the two proteoglycans is higher than that of the efficacy raw materials combined in pairs. Through the synergistic effect of the functional compositions, the expression of two proteins with sugar is obviously increased, so that the collagen fiber is thinner, and the fiber quality and strength are better.

Pouch-removing effect test example 2

1) The tester: 32 healthy people (20-45 years old), female: the face has no obvious redness, skin damage, scars and other conditions;

2) test samples and sites: example 1; the periphery of the eye;

3) testing an instrument: primos is used for facial image acquisition and wrinkle and pouch analysis;

4) testing indexes are as follows: the pouch volume; wherein, the rate of change is (post-use measurement-pre-use measurement) ÷ pre-use measurement × 100%;

the test results are shown in FIGS. 3-5 and tables 4-6 below:

table 4 shows the comparison of the volume values of the anterior and posterior pouches using the eye cream composition of example 1

	Before use	After 7 days of use	After 14 days of use	After 28 days of use	After 42 days of use
						Example 1	73.74	66.13	64.3	67.97	61.32
Blank example	74.89	81.15	73.16	75.89	75.25

Compared with the prior use: after 7 days of sample application, the pouch volume in the sample area was significantly reduced by 7.61; after 14 days of sample application, the pouch volume in the sample area was significantly reduced by 9.44; there was no significant decrease in pouch volume in the sample area by 5.77 days after using the sample; the pouch volume in the sample area was significantly reduced by 12.42 days after using the sample.

Table 5 change value before use of the eye cream composition of example 1 compared to before use

	After 7 days of use	After 14 days of use	After 28 days of use	After 42 days of use
					Example 1	-7.61	-9.44	-5.77	-12.42
Blank example	6.26	-1.73	1.00	0.36

Table 6 rate of change before use of the eye cream composition of example 1

	After 7 days of use	After 14 days of use	After 28 days of use	After 42 days of use
					Example 1	-10.32％	-12.80％	-7.83％	-16.85％
Blank example	8.36％	-2.31％	1.34％	0.48％

Meanwhile, the calculation results of the change rate show that: after 7 days of sample application, the pouch volume in the sample area was significantly reduced by 10.32%, and the pouch volume in the control area was not significantly increased by 8.36%; after 14 days of sample application, the pouch volume in the sample area was significantly reduced by 12.80%, and the pouch volume in the control area was not significantly reduced by 2.31%; after 28 days of sample application, the pouch volume in the sample area was significantly reduced by 7.83%, and the pouch volume in the control area was not significantly increased by 1.34%; after 42 days of sample application, the pouch volume in the sample area was significantly reduced by 16.85%, and the pouch volume in the control area was not significantly increased by 0.48%.

As can be seen from the data in FIGS. 3-5 and tables 4-6, the pouch volume reducing effect of example 1 of the present invention is significant, and the pouch removing effective ingredient used in the present invention has a very good effect.

Ophthalmologist clinical evaluation test 3

1. The tester: 30 healthy people (20-45 years old), female: the face has no obvious redness, skin damage, scars and other conditions;

2. testing parts: the periphery of the eye;

3. the evaluation criteria are as follows in table 7:

TABLE 7 ophthalmic clinical evaluation score criteria Table

4. The test results are shown in table 8 below:

TABLE 8 results of ophthalmic clinical evaluation

During the use of the samples, 0 subjects had adverse reactions at the sites of eyelids, lid margin and eyelashes, palpebral conjunctiva, cornea, anterior chamber, light reflex, and the like.

Safety verification test example 4

1) The number of tested persons: and (5) trial sample formula for 30 healthy people (20-45 years old).

2) Testing parts: the curved side of the forearm;

3) the test method comprises the following steps: the selection area is not more than 50mm²A suitable spot tester with a depth of about 1mm for closed spot testThe test method comprises adding about 0.020-0.025 mL (g) of the test substance into a spot tester. The patch tester is applied to the curved side of the forearm of the subject, the subject is removed 24 hours later, the skin reaction is observed 0.5 hour, 24 hours and 48 hours after the removal, and the result is recorded according to the skin reaction grading standard in the technical Specification for cosmetic safety 2015.

4) The evaluation criteria are as follows in table 9:

TABLE 9 grading Standard of skin reactions

The results show that: the result of the human skin patch experiment shows that the score grades of 0.5h, 24h and 48h of 3 observation time points of 30 subjects after the patch is removed are all 0. The subject did not cause adverse skin reactions to the subjects of this batch as specified in the technical Specification for cosmetic safety (2015 edition).

Synergistic test example 5

1) The tester: 90 healthy people (20 ~ 45 years old), 9 groups, 10 people of every group, women: the face has no obvious redness, skin damage, scars and other conditions; example 1 and comparative examples 1 to 8 were used, respectively;

2) testing parts: the periphery of the eye;

3) testing an instrument: primos is used for facial image acquisition and wrinkle and pouch analysis;

4) testing indexes are as follows: pouch volume.

The test results are shown in tables 10-11 below:

TABLE 10 comparison of before and after eye pouch volume values using the eye cream compositions of example 1 and comparative examples 1-8

TABLE 11 change in pouch volume before and after use of the eye cream compositions of example 1 and comparative examples 1 to 8

	After 7 days of use	After 14 days of use	After 28 days of use	After 42 days of use
					Example 1	-7.06	-8.96	-10.07	-12.54
Comparative example 1	-4.53	-4.81	-5.06	-5.37
					Comparative example 2	-4.28	-4.52	-4.84	-5.03
Comparative example 3	-4.11	-4.32	-4.79	-4.91
					Comparative example 4	-4.47	-4.76	-4.93	-5.12
Comparative example 5	-4.02	-4.29	-4.67	-4.85
					Comparative example 6	-3.59	-3.78	-4.05	-4.11
Comparative example 7	-3.37	-3.64	-3.96	-4.05
					Comparative example 8	-3.86	-3.97	-4.16	-4.23

The pouch-removing effect test was performed on example 1 and comparative examples 1 to 8, and the test results are shown in tables 10 to 11.

As can be seen from table 10, after 7 days of use, the pouch volume sizes are ranked as: comparative examples 7>6>8>5>3>2>4>1> example 1; after 14 days of use, pouch volume sizes were ranked as: comparative example 1>2>4>3> example 1; after 28 days of use, pouch volume sizes were ranked as: comparative examples 7>6>8>5>3>2>4>1> example 1; after 42 days of use, pouch volume sizes were ranked as: comparative examples 7>6>8>5>3>2>4>1> example 1.

As can be seen from table 11, after 7 days of use, the reduction in pouch volume values are ranked as: example 1> comparative example 1>4>2>3>5>8>6> 7; after 14 days of use, the reduction in pouch volume values were ranked as: example 1> comparative example 1>4>2>3>5>8>6> 7; after 28 days of use, the pouch volume reduction values were ranked as: example 1> comparative example 1>4>2>3>5>8>6> 7; the pouch volume reduction values are ordered as: example 1> comparative example 1>4>2>3>5>8>6> 7.

The results of each period of use show that the pouch volume reduction values of example 1 are significantly greater than those of the control group, which indicates that the four effective components of the invention have synergistic pouch removing effects.

The eye pouch removing effect composition consisting of the kappaphycus alvarezii extract, the tetradecyl amino butyryl valinamide trifluoroacetate dipeptide diaminobutyryl benzylamide diacetate and the acetyl tetrapeptide-5 can synergistically exert the eye pouch removing effect and realize mutual synergism. Firstly, the kappaphycus alvarezii extract can effectively replenish water, moisturize, relieve dry skin relaxation and inhibit melanin generation; the acetyl tetrapeptide-5 can diminish inflammation and eliminate eye edema by changing vascular permeability, and then the tetradecyl amino butyryl valinamide butyric urea trifluoroacetate, the dipeptide diaminobutyryl benzyl amide diacetate and the acetyl tetrapeptide-5 exert the effects of tightening skin and improving skin elasticity, and the eye pouch dispelling effect is improved by the synergistic effect, and meanwhile, the eye fine wrinkles and melanin deposition fading effect are achieved.

In conclusion, the pouch removing effect compound has a relatively obvious pouch removing effect through the synergistic effect of the components, and the pouch removing effect is more obvious than that of the pouch removing effect of the combination of two or three components.

The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, so that any simple modification, equivalent change and modification made to the above embodiment according to the technical spirit of the present invention are within the scope of the technical solution of the present invention.