阅读说明：本技术 一种具有分液功能的多联排管盖 (Multi-row pipe cover with liquid separating function ) 是由 李�东 于 2021-10-22 设计创作，主要内容包括：本发明公开了一种具有分液功能的多联排管盖,涉及试剂管技术领域。通过一步加样工作,实现将试剂加入到多联排的试剂管中,从而节省操作人员工作量,解决了大批量加注时存在的操作人员工作强度大、工作效率低的问题。多联排管盖包括管盖,所述管盖内设有分液模块以及于分液模块连通的加液单元,所述分液模块包括若干依次设置且互相连通的分液单元；所述分液单元包括底部开设有离心孔的储液池,相邻储液池的顶部通过微流控通道保持连通。通过离心力将分液模块中的样本液分流到每个试剂管中,从而避免操作人员将相同的试剂加入到多个试剂管中,节省了操作人员的工作量。(The invention discloses a multi-row tube cover with a liquid separating function, and relates to the technical field of reagent tubes. Through one-step application of sample work, realize adding reagent to the reagent pipe that ally oneself with row more to save operating personnel work load, solved the operating personnel working strength who exists when filling in batches big, problem that work efficiency is low. The multi-connected pipe discharging cover comprises a pipe cover, a liquid separating module and a liquid adding unit communicated with the liquid separating module are arranged in the pipe cover, and the liquid separating module comprises a plurality of liquid separating units which are sequentially arranged and communicated with each other; the liquid separating unit comprises liquid storage tanks with centrifugal holes at the bottoms, and the tops of adjacent liquid storage tanks are communicated through a microfluidic channel. Through the centrifugal force with divide the sample liquid reposition of redundant personnel in the liquid module to every reagent pipe to avoid operating personnel to add the same reagent in a plurality of reagent pipes, saved operating personnel's work load.)

1. The utility model provides a many allies oneself with calandria lid with divide liquid function, its characterized in that, includes tube cap (7), be equipped with in tube cap (7) and divide the liquid module and in the liquid feeding unit that divides liquid module intercommunication, divide the liquid module to include a plurality of minute liquid units (8) that set gradually and communicate each other.

2. The cover with the function of separating liquid of claim 1, wherein the separating unit (8) comprises liquid reservoirs (5) with centrifugal holes (501) at the bottom, and the tops of adjacent liquid reservoirs (5) are kept communicated through the microfluidic channel (4).

3. The multi-row pipe cover with the liquid separation function according to claim 2, wherein a rotary channel is further formed in the middle of the centrifugal hole (501), and the depth of the centrifugal hole (501) is 0.1 mm-50 mm.

4. The multi-row tube cover with the liquid separating function according to claim 1, wherein the liquid adding unit comprises a sample adding hole (9) and an exhaust hole (10), and the sample adding hole (9) and the exhaust hole (10) are respectively positioned at two ends of the liquid separating module and are communicated with the liquid separating units (8) positioned at the two ends.

5. The cover of the multi-row pipe with the function of separating liquid as claimed in claim 4, wherein the cross section of the sample adding hole (9) and the cross section of the vent hole (10) are both circular, and the bottoms of the sample adding hole (9) and the vent hole (10) are both provided with waves or spurs (901).

6. The multi-row tube cover with the liquid separation function according to claim 4, wherein the tube cover (7) comprises a tube cover main body (2) and a cover plate (1), the sample adding holes (9) and the exhaust holes (10) are respectively arranged at two ends of the cover plate (1), the liquid storage tank (5) is arranged on the tube cover main body (2), the microfluidic channel (4) is arranged on the tube cover main body (2) or the cover plate (1), and the sample adding holes (9) and the exhaust holes (10) are respectively communicated with the microfluidic channels (4) at two ends of the tube cover main body (2) or the cover plate (1).

7. The multi-row pipe cover with the liquid separating function according to any one of claims 1 to 6, wherein the pipe cover (7) is a strip-shaped pipe cover, one or more groups of liquid adding units and liquid separating modules are arranged, the liquid adding units and the liquid separating modules in each group are distributed in a straight line shape, and the central lines of the liquid adding units and the liquid separating modules in each group are coincident with or parallel to the central line of the pipe cover (7).

8. The cover of a multi-gang duct with the function of separating liquid according to any of claims 1-6, wherein the separating unit (8) further comprises a blocking valve (3) arranged in the microfluidic channel (4), and the width of the blocking valve (3) is smaller than that of the microfluidic channel (4).

9. The cover of the multi-row pipe with the function of separating liquid as claimed in any one of claims 1 to 6, wherein the reservoir (5) is a bucket reservoir, the top of the bucket reservoir is communicated with the microfluidic channel (4), and the centrifugal hole (501) is located at the center of the bottom of the bucket reservoir.

10. The cover of the multi-row pipe with the function of separating liquid as claimed in any one of claims 1 to 6, wherein the cross-sectional area of the microfluidic channel (4) is 0.1mm²~50 mm²The liquid storage tank (5) can store liquid with the volume of 0.1-200 mu L, the centrifugal hole (501) is round, and the radius is 0.1-5 mm.

Technical Field

The invention relates to the technical field of reagent tubes, in particular to a multi-row tube cover with a liquid separating function.

Background

The row of concatenating is a laboratory consumptive material commonly used, and it is a consumptive material that links together a plurality of reagent pipings, can make things convenient for the experimenter to take, operate etc. when experimental work load is very big, and the row of concatenating generally divide into 2 parts, and partly is the pipe shaft, and partly is the tube cap. The common multi-row comprises eight rows, 96 pore plates and 384 pore plates.

In the experimental process, operators face the situation that the same reagent is added into a plurality of reagent tubes, and the operators are required to add the same reagent into a plurality of reagent tubes in a plurality of commercialized detection reagent kits, so that the repeated work of the operators is increased, particularly in medical institutions and detection institutions, and the situation is more.

Disclosure of Invention

Aiming at the problems, the invention provides the multi-row tube cover with the liquid separating function, and the reagent is added into the multi-row reagent tubes through one-step sample adding work, so that the workload of operators is saved, and the problems of high working strength and low working efficiency of the operators during mass sample adding are solved.

The technical scheme of the invention is as follows: the multi-connection calandria cover comprises a pipe cover 7, a liquid separating module and a liquid adding unit communicated with the liquid separating module are arranged in the pipe cover 7, and the liquid separating module comprises a plurality of liquid separating units 8 which are sequentially arranged and mutually communicated. Through set up the liquid feeding unit in the tube cap and divide the liquid module, can make the tube cap have the function of dividing the liquid, during the application of sample, it is fixed with a plurality of reagent pipes with the tube cap, operating personnel is through the one-step application of sample to the tube cap of liquid with the liquid feeding unit afterwards, put into centrifuge with tube cap and reagent pipe centrifugation after the application of sample, will divide the sample liquid in the liquid module to divide to every reagent pipe through centrifugal force, thereby avoid operating personnel to add the same reagent to a plurality of reagent pipes, operating personnel's work load has been saved.

Further, the liquid separating unit 8 comprises liquid storage pools 5 with centrifugal holes 501 formed at the bottoms, and the tops of adjacent liquid storage pools 5 are kept communicated through the microfluidic channels 4. After the sample liquid passed through the application of sample of liquid unit to the tube cap, the liquid reserve tank was added to the micro-fluidic channel of sample liquid through a plurality of intercommunications, and there is the reagent pipe that needs to add the sample liquid every liquid reserve tank below, blocks up the liquid application unit of tube cap afterwards, puts into centrifugal device with tube cap and reagent pipe again and carries out the centrifugation, can make the sample liquid in the liquid reserve tank enter into the reagent pipe through the centrifugation hole through the effect of centrifugal force, accomplishes and divides the liquid.

Furthermore, a rotary channel is further formed in the middle of the centrifugal hole 501, and the depth of the centrifugal hole 501 is 0.1 mm-50 mm. In other words, the centrifugal hole 501 may have a certain depth; in addition, in order to increase the air resistance, a certain rotation can be provided.

Further, the liquid adding unit comprises a sample adding hole 9 and an exhaust hole 10, wherein the sample adding hole 9 and the exhaust hole 10 are respectively located at two ends of the liquid separating module and communicated with the liquid separating units 8 located at the two ends. Because the sample adding hole is communicated with the air vent through the plurality of communicated liquid separating units, when the sample liquid is added into the tube cover through the sample adding hole, air in the liquid separating unit is exhausted from the air vent and is replaced by the sample liquid.

Further, the cross sections of the sampling hole 9 and the exhaust hole 10 are both circular, and the bottoms of the sampling hole 9 and the exhaust hole 10 are both provided with waves or spurs 901. The radius scope that adds appearance hole and exhaust hole is 0.01mm to 5mm, all set up wave or spur structure bottom adding appearance hole and exhaust hole, can make sample liquid add from the arbitrary one end of tube cap, when adding sample liquid simultaneously, because wave or spur structure, when carrying out the liquid feeding through the liquid-transfering gun, the rifle head of liquid-transfering gun has certain space with the bottom that adds appearance hole or exhaust hole, make sample liquid can not blockked up, thereby be favorable to the application of sample liquid.

Further, the tube cap 7 comprises a tube cap main body 2 and a cover plate 1, the two ends of the cover plate 1 are respectively provided with a sample adding hole 9 and an exhaust hole 10, the liquid storage tank 5 is arranged on the tube cap main body 2, the microfluidic channel 4 is arranged on the tube cap main body 2 or the cover plate 1, and the sample adding hole 9 and the exhaust hole 10 are respectively communicated with the microfluidic channels 4 at the two ends of the tube cap main body 2 or the cover plate 1. The tube cover is divided into a tube cover main body and a cover plate, and the microfluidic channel is arranged on the tube cover main body or the cover plate, so that the liquid adding unit or the liquid separating unit in the tube cover can be easily cleaned when being blocked.

Further, tube cap 7 is the bar tube cap, and the quantity of liquid feeding unit and branch liquid module has a set of or multiunit, and the liquid feeding unit of every group all is a style of calligraphy with dividing the liquid module and distributing, and the liquid feeding unit of every group all coincides or is parallel with 7 central lines of tube cap with dividing the liquid module central line. The strip-shaped pipe cover enables the liquid adding unit and the liquid separating module to be in a linear distribution structure, so that liquid adding is easier. The liquid adding unit and the liquid separating module can be arranged along the central line of the strip-shaped pipe cover and also can be arranged on one side of the central line of the pipe cover. Or a plurality of groups of liquid adding units and liquid separating modules which are parallel or coincident with the central line of the pipe cover can be arranged on the pipe cover. The liquid feeding unit of a set of or multiunit with divide liquid module, the central line of every group all coincides or is parallel with the tube cap central line, set up multiunit liquid feeding unit and divide liquid module and can add the sample liquid that kind and application of sample unit quantity are the same at the tube cap, carry out centrifugation afterwards, can further reduce loaded down with trivial details nature when dividing liquid.

Further, the liquid separation unit 8 further comprises a blocking valve 3 arranged in the microfluidic channel 4, and the width of the blocking valve 3 is smaller than that of the microfluidic channel 4. Because the thickness of the separating valve is smaller than that of the microfluidic channel, a gap is reserved between the separating valve and the microfluidic channel, and the separating valve with the thickness smaller than that of the microfluidic channel is arranged, on one hand, the sample liquid can be smoothly filled, on the other hand, when the sample liquid is subjected to centrifugal liquid separation, the separating valve has a separating effect on the sample liquid, and the amount of the sample liquid obtained by each reagent tube is more average.

Further, the width of the microfluidic channel 4 gradually decreases from the position of the reservoir 5 to the position of the blocking valve 3. Therefore, the sample liquid is retained in the liquid storage tank when the sample liquid is added, and the microfluidic channel plays a certain role in separating the liquid from the sample liquid, so that the liquid separation is more even.

Further, the liquid storage tank 5 is a bucket type liquid storage tank, the top of the bucket type liquid storage tank is communicated with the microfluidic channel 4, and the centrifugal hole 501 is located in the center of the bottom of the bucket type liquid storage tank. Set up the bucket type liquid storage tank and can make easier when dividing liquid, during centrifugal separation, sample liquid in the bucket type liquid storage tank gets into the test tube through the centrifugation hole.

Further, the cross-sectional area of the microfluidic channel 4 is 0.1mm²~50 mm²The liquid storage tank 5 can store liquid with a volume of 0.1-200 μ L, the centrifugal hole 501 is circular, and the radius is 0.1-5 mm.

The invention has the following beneficial effects:

one, through set up the liquid feeding unit in the tube cap and divide the liquid module, can make the tube cap have the function of dividing the liquid, during the application of sample, it is fixed with a plurality of reagent pipes with the tube cap, operating personnel is through the one-step application of sample to the tube cap of liquid feeding unit with the sample liquid afterwards, put into centrifuge with tube cap and reagent pipe centrifugation after the application of sample, will divide the sample liquid that divides in the liquid module to every reagent pipe through centrifugal force, thereby avoid operating personnel to add the same reagent to a plurality of reagent pipes, operating personnel's work load has been saved.

And secondly, the tube cover is divided into a tube cover main body and a cover plate, and the microfluidic channel is arranged on the tube cover main body or the cover plate, so that the liquid adding unit or the liquid separating unit in the tube cover can be easily cleaned when being blocked.

And the liquid adding units and the liquid separating modules are distributed in a straight line shape, so that liquid adding and liquid separating are easier. The liquid separating unit can be arranged along the central line of the strip-shaped pipe cover and also can be arranged on one side of the central line of the pipe cover. And a plurality of groups of liquid adding units and liquid separating modules which are arranged side by side can also be arranged on the pipe cover. Set up multiunit liquid feeding unit and divide the liquid module and can add the sample liquid that kind and application of sample unit quantity are the same at the tube cap, carry out centrifugation once afterwards, loaded down with trivial details nature when can further reducing dividing liquid.

And fourthly, by arranging the blocking valve with the width smaller than that of the microfluidic channel, when the sample liquid is subjected to centrifugal liquid separation, the blocking valve has a blocking effect on the sample liquid, so that the amount of the sample liquid obtained by each reagent tube is more average.

And fifthly, the area of the cross section of the microfluidic channel from the middle part to the two ends is gradually reduced, so that the sample liquid is retained in the liquid storage tank when the sample liquid is added, and the microfluidic channel plays a certain role in separating the liquid when the sample liquid is separated, so that the liquid separation is more average.

Sixthly, all set up wave or spur structure bottom adding the appearance hole and exhausting hole, can be so that sample liquid can add from the arbitrary one end of tube cap, when adding sample liquid simultaneously, because wave or spur structure, when carrying out the liquid feeding through the liquid-transfering gun, the rifle head of liquid-transfering gun has certain space with the bottom of appearance hole or exhausting hole for sample liquid can not blockked up, thereby is favorable to the application of sample liquid.

Drawings

FIG. 1 is a schematic front view of the present invention;

FIG. 2 is a schematic front view of a dispensing unit of the present invention;

FIG. 3 is a schematic top view of a dispensing unit of the present invention;

FIG. 4 is a schematic diagram of a microfluidic channel of the present invention disposed on a cover plate;

FIG. 5 is a schematic view of a microfluidic channel of the present invention disposed on a cap body;

FIG. 6 is a schematic view of a well structure according to the present invention;

FIG. 7 is an enlarged view of a portion of FIG. 2 at A;

FIG. 8 is a schematic diagram of an optimized structure of a centrifugal hole,

FIG. 9 is a schematic diagram of a further optimized centrifugal hole structure.

Reference numerals:

1-cover plate, 2-tube cap main body, 3-block valve, 4-microfluidic channel, 5-liquid storage pool, 501-centrifugal hole, 6-test tube sleeve, 7-tube cap, 8-liquid separation unit, 9-sample adding hole, 901-spurt, 10-vent hole and 11-reagent tube.

Detailed Description

In order to clearly explain the technical features of the present patent, the following detailed description of the present patent is provided in conjunction with the accompanying drawings.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "longitudinal", "lateral", "horizontal", "inner", "outer", "front", "rear", "top", "bottom", and the like indicate orientations or positional relationships that are based on the orientations or positional relationships shown in the drawings, or that are conventionally placed when the product of the present invention is used, and are used only for convenience in describing and simplifying the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus should not be construed as limiting the invention.

In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "open," "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

The invention is explained in detail below with reference to the figures and with reference to embodiments:

referring to fig. 1 and 2, a multi-row tube cap with liquid separating function comprises a tube cap 7, wherein a liquid adding unit and a liquid separating module are arranged in the tube cap 7, the liquid separating module comprises a plurality of liquid separating units 8 which are sequentially arranged and communicated with each other, and the liquid adding unit is communicated with the liquid separating units 8. Through set up the liquid feeding unit in tube cap 7 and divide the liquid module, can make tube cap 7 have the function of dividing the liquid, during the application of sample, fix tube cap 7 and a plurality of reagent pipe 11, operating personnel is at any time through the one step application of sample of liquid to tube cap 7 of liquid feeding unit, put into centrifuge with tube cap 7 and reagent pipe 11 after the application of sample in centrifugation, will divide the sample liquid in the liquid module to divide to every reagent pipe 11 through centrifugal force, thereby avoid operating personnel to add the same reagent in a plurality of reagent pipes 11, operating personnel's work load has been saved. The liquid separating unit 8 of this embodiment is eight in quantity, take multigene locus detection to carry out the PCR test as an example, test hundreds of cases and even thousands of people's samples, everyone's sample need add respectively in 8 different pipes, the PCR reagent that can survey 8 gene loci has been added in the pipe according to traditional application of sample method, everyone's sample need add in 8 different pipes, just need operate 8 times, add 8000 times if 1 thousand samples, but, adopt the multiple gang calandria lid 7 that has liquid separating function of this design, can only add once, can divide in 8 different pipes, make the work load of dividing the liquid reduce 8 times, personnel's work load has been saved greatly.

The liquid separating unit 8 comprises a microfluidic channel 4 and a liquid storage tank 5, the microfluidic channel 4 of the liquid separating unit 8 is communicated with each other, the top of the liquid storage tank 5 is communicated with the microfluidic channel 4, and the bottom of the liquid storage tank 5 is provided with a centrifugal hole 501. After the sample liquid added the sample to tube cap 7 through the liquid feeding unit, the liquid storage tank 5 was added to the micro-fluidic channel 4 through a plurality of intercommunications to the sample liquid, there is reagent pipe 11 that needs to add the sample liquid in every liquid storage tank 5 below, block up the liquid feeding unit of tube cap 7 afterwards, put into centrifugal device with tube cap 7 and reagent pipe 11 again and carry out the centrifugation, can make the sample liquid in the liquid storage tank 5 enter into reagent pipe 11 through centrifugation hole 501 through the effect of centrifugal force, accomplish and divide liquid.

The centrifugal hole 501 shown in fig. 2 and 7 may also have a certain depth, as shown in fig. 8; in addition, to increase the air resistance, there may be a certain amount of rotation, as shown by the circular area in FIG. 9. The total length or depth of the centrifugal hole 501 may be 0.1mm to 50 mm.

The liquid adding unit comprises a sample adding hole 9 and an exhaust hole 10, wherein the sample adding hole 9 and the exhaust hole 10 are respectively located at two ends of the liquid separating module and communicated with the liquid separating units 8 located at the two ends. Since the sample addition hole 9 and the air discharge hole 10 are communicated through the plurality of communicated liquid separation units 8, when the sample liquid is added to the tube cap 7 through the sample addition hole 9, air in the liquid separation unit 8 is discharged from the air discharge hole 10 and replaced by the sample liquid.

The pipe cap 7 is the bar pipe cap, and the quantity of liquid feeding unit and branch liquid module has a set of or multiunit, and the liquid feeding unit of every group all is a style of calligraphy with dividing the liquid module and distributing, and the liquid feeding unit of every group all coincides or is parallel with 7 central lines of pipe cap with dividing liquid module central line. The strip-shaped pipe cover 7 enables the liquid adding unit and the liquid separating module to be in a linear distribution structure, so that liquid adding is easier. The liquid separating unit 8 can be arranged along the central line of the strip-shaped pipe cover 7, and can also be arranged on one side of the central line of the pipe cover 7. A plurality of groups of liquid adding units and liquid separating modules which are arranged side by side can also be arranged on the tube cover 7. The liquid feeding unit of a set of or multiunit with divide liquid module, the central line of every group all coincides or is parallel with 7 central lines of tube cap, set up multiunit liquid feeding unit and divide liquid module and can add the sample liquid that kind and application of sample unit quantity are the same at tube cap 7, carry out centrifugation afterwards, loaded down with trivial details nature when can further reduce dividing liquid.

Referring to fig. 4 and 5, the tube cap 7 includes a tube cap main body 2 and a cover plate 1, the sample adding hole 9 and the air vent 10 are respectively disposed at two ends of the cover plate 1, the liquid storage tank 5 is disposed on the tube cap main body 2, the microfluidic channel 4 is disposed on the tube cap main body 2 or the cover plate 1, and the sample adding hole 9 and the air vent 10 are respectively communicated with the microfluidic channels 4 disposed at two ends of the tube cap main body 2 or the cover plate 1. The tube cover 7 is divided into the tube cover main body 2 and the cover plate 1, and the microfluidic channel 4 is arranged on the tube cover main body 2 or the cover plate 1, so that the liquid adding unit or the liquid separating unit 8 in the tube cover 7 can be easily cleaned when being blocked.

As shown in fig. 2 and 7, the liquid separation unit 8 further includes a blocking valve 3, and a width or a thickness of the blocking valve 3 is smaller than a width or a thickness of the microfluidic channel 4, so that a gap is left between the blocking valve 3 and the microfluidic channel 4, and when the sample liquid is subjected to centrifugal liquid separation, the blocking valve 3 has a blocking effect on the sample liquid, so that the amount of the sample liquid separated by each reagent tube 11 is more average.

The liquid storage tank 5 is a bucket type liquid storage tank 5, the top of the bucket type liquid storage tank 5 is communicated with the microfluidic channel 4, and the centrifugal hole 501 is positioned in the center of the bottom of the bucket type liquid storage tank 5. The bucket type liquid storage tank 5 is arranged so that liquid separation is easier, and during centrifugal separation, sample liquid in the bucket type liquid storage tank 5 enters the test tube through the centrifugal hole 501.

As shown in fig. 3, the cross-section of the microfluidic channel 4 gradually decreases in area from the middle to both ends. The area of the cross section of the microfluidic channel 4 from the middle part to the two ends is gradually reduced, so that the sample liquid is retained in the liquid storage tank 5 when the sample liquid is added, and the microfluidic channel 4 plays a certain role in blocking when the sample liquid is separated, so that the liquid separation is more even.

As shown in fig. 6, the cross sections of the sample adding hole 9 and the vent hole 10 are both circular, and the bottoms of the sample adding hole 9 and the vent hole 10 are both provided with a wave or a spur 901 structure. The radius scope of adding appearance hole 9 and exhaust hole 10 is 0.01mm to 5mm, all set up wave or bur 901 structure bottom adding appearance hole 9 and exhaust hole 10, can make sample liquid can add from the arbitrary one end of tube cap 7, when adding sample liquid simultaneously, because wave or bur 901 structure, when carrying out the liquid feeding through the liquid-transfering gun, the rifle head of liquid-transfering gun has certain space with the bottom of adding appearance hole 9 or exhaust hole 10, make sample liquid can not blockked up, thereby be favorable to the application of sample liquid.

The cross-sectional area of the microfluidic channel 4 is 0.1mm²~50mm²The liquid storage tank 5 can store liquid with a volume of 0.1-200 μ L, the centrifugal hole 501 is circular, and the radius is 0.1-5 mm.

The working principle of the invention is as follows:

when the device is used, a sample liquid is added into the tube cover 7 through the sample adding hole 9 by the pipette, and because the bottom of the sample adding hole 9 is provided with a wave or bulge structure, a gap is reserved between the gun head of the pipette and the bottom of the sample adding hole 9, so that the sample liquid is prevented from being blocked when in sample adding, because the sample adding hole 9 is communicated with the air vent hole 10 through the liquid separating unit 8, air in the liquid separating unit 8 is discharged from the air vent hole 10 when in sample adding, because the cross-sectional area of the microfluidic channel 4 of the liquid separating unit 8 is gradually reduced from the middle part to the end part when in sample adding, the sample liquid can be more easily retained in the liquid storage pool 5, after the sample adding is completed, the air in the liquid separating unit 8 is replaced by the sample liquid, then the sample adding hole 9 and the air vent hole 10 are blocked, then the tube cover 7 and the reagent tube 11 are placed into a centrifuge for centrifugation, and because of the centrifugal force, the sample liquid in the liquid storage pool 5 in the liquid separating unit 8 enters the reagent tube 11 through the centrifugal hole 501, during centrifugation, the separating valve 3 is arranged in the microfluidic channel 4 of the liquid separating unit 8, and the width of the microfluidic channel 4 is gradually reduced from the position of the liquid storage tank 5 to the position of the separating valve 3. Therefore, when centrifugal separation is performed, the sample liquid is blocked, so that the sample liquid entering each reagent tube 11 is relatively balanced.

While the invention has been described in terms of its preferred embodiments, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention.