阅读说明：本技术 观察用细胞培养装置 (Cell culture device for observation ) 是由 王亮 盛嘉安 于 2021-09-06 设计创作，主要内容包括：本发明提供了一种观察用细胞培养装置,包括培养皿主体、培养皿适配板、水浴盖板以及滑盖,培养皿主体包括壳体及与布置在壳体内侧且与壳体相连的立板,立板的外侧面与壳体之间形成第一容纳空间,立板的内侧面与壳体的部分围成第二容纳空间；培养皿适配板匹配安装到第二容纳空间中并通过立板所具有的立板边沿支撑,滑盖的两侧可滑动的安装在壳体两侧的滑槽,水浴盖板安装在立板顶端,滑盖上设置有加热玻璃且具有打开和关闭两种状态,在关闭状态时加热玻璃处于通电状态,在打开状态时加热玻璃处于断电状态,本发明通过滑动实现触点接触的方式实现加热玻璃通断电进而实现对培养皿加热,可保持稳定的样品温度,结构简单,实用性强。(The invention provides a cell culture device for observation, which comprises a culture dish main body, a culture dish adapter plate, a water bath cover plate and a sliding cover, wherein the culture dish main body comprises a shell and a vertical plate which is arranged on the inner side of the shell and connected with the shell, a first accommodating space is formed between the outer side surface of the vertical plate and the shell, and a second accommodating space is enclosed by the inner side surface of the vertical plate and the shell; the culture dish adapter plate is installed in the second containing space in a matched mode and supported by the edge of the vertical plate, the two sides of the sliding cover can be installed on the sliding grooves on the two sides of the shell in a sliding mode, the water bath cover plate is installed at the top end of the vertical plate, the heating glass is arranged on the sliding cover and has two states of opening and closing, the heating glass is in a power-on state in the closing state, and the heating glass is in a power-off state in the opening state.)

1. A cell culture device for observation is characterized by comprising a culture dish main body (1), a culture dish adapter plate (2), a water bath cover plate (3) and a sliding cover (4);

the culture dish main body (1) comprises a shell (101) and a vertical plate (102) which is arranged on the inner side of the shell (101) and connected with the shell (101), wherein a first accommodating space (103) is formed between the outer side surface of the vertical plate (102) and the shell (101), and a second accommodating space (104) is enclosed by the inner side surface of the vertical plate (102) and the part of the shell (101);

the culture dish adapting plate (2) can be matched and installed in the second accommodating space (104) and is supported by a vertical plate edge (105) extending towards the inner side from the bottom end of the vertical plate (102), and the culture dish adapting plate (2) is used for installing a culture dish;

the top ends of two sides of the shell (101) are respectively provided with sliding chutes (1011) which are oppositely arranged, two sides of the sliding cover (4) are slidably arranged in the two sliding chutes (1011), and the water bath cover plate (3) is arranged at the top end of the vertical plate (102) and is arranged below the sliding cover (4);

the sliding cover (4) has two states of opening and closing, the heating glass (5) is arranged on the sliding cover (4), when the sliding cover (4) is in the closed state, the heating glass (1) is in the power-on state, and when the sliding cover (4) is in the open state, the heating glass (1) is in the power-off state.

2. The cell culture device for observation according to claim 1, wherein a magnetic attraction structure (41) is provided at an end of the sliding cover (4), and a structure contact (1012) matching with the magnetic attraction structure (41) is provided on the housing (101);

when the sliding cover (4) is in a closed state, the magnetic attraction structure (41) is connected with the structure contact (1012).

3. The cell culture apparatus for observation according to claim 1, further comprising a control mechanism, a first temperature sensor, a second temperature sensor, and a heater;

the first accommodating space (103) is used for containing water, the heater is arranged outside the shell (101) and faces the first accommodating space (103), and the first temperature sensor is arranged in the first accommodating space (103);

the first temperature sensor, the second temperature sensor, the heater and the heating glass (5) are respectively electrically connected with the control mechanism.

4. The observation cell culture apparatus according to claim 3, wherein the control means is capable of controlling the current flowing through the heater and the heating glass (5) based on a preset target temperature range and temperature information obtained from the first temperature sensor and the second temperature sensor.

5. The observation cell culture apparatus according to claim 3, wherein the first housing space (103) is a U-shaped space and surrounds the outside of the second housing space (104);

the shell (101) is provided with a first flow passage (106) communicated with the U-shaped space, wherein the first flow passage (106) is used for supplying air.

6. The observation cell culture apparatus according to claim 5, wherein the first flow channel (106) is connected to a first valve at one end outside the housing (101), and the control mechanism is in signal connection with the first valve.

7. The cell culture apparatus for observation according to claim 1, wherein an observation window (108) is provided in the housing (101), and the observation window (108) is used for observing the liquid level in the first accommodation space (103).

8. The observation cell culture apparatus according to claim 1, further comprising a microscope adapter base plate (6), wherein the culture dish body (1) is fittingly mounted in a C-shaped space provided in the microscope adapter base plate (6) and supported by a base plate rim (61) inside the microscope adapter base plate (6).

9. The cell culture device for observation according to claim 1, wherein one or more notches (7) are arranged at the top edge of the vertical plate (102) at intervals so that the first accommodating space (103) is communicated with the second accommodating space (104) through the notches (7) when the water bath cover plate (3) is covered on the top end of the vertical plate (102).

10. The cell culture apparatus for observation according to claim 1, wherein the petri dish adapter plate (2) comprises a single-well as a multi-well structure.

Technical Field

The invention relates to the technical field of cell culture, in particular to a cell culture device for observation.

Background

Cell culture refers to a method for simulating in vivo environment in vitro to enable the cell to survive, grow and reproduce and maintain main structure and function, and is also called cell cloning technology, and cell culture technology is also called cloning technology, and is an essential process for the whole field of bioengineering.

Present cell culture equipment on the market all accompanies microscope observation when cultivateing to ensure that cell culture process normally goes on, in the culture process of cell, cell culture receives the influence of environment very strong, not only needs sterile environment, still need to change the air to suitable temperature and timely, but need exchange with outside air in order to prevent that inside carbon dioxide concentration is too high, then can lead to inside air temperature fluctuation during the air exchange, lead to cell death or slow down the speed of cultivation, therefore how to realize the constant temperature or the temperature within a certain range is invariable to seem to be especially important.

Patent document CN212451447U discloses a cell culture equipment for microscope, including the cell culture jar, the inner chamber diapire fixed mounting of cell culture jar has the piece that generates heat, the inside wall fixed mounting of cell culture jar has the go-between, the inboard fixed mounting of go-between has the box that holds, the inner chamber diapire fixed mounting who holds the box has the storage rack, the top movable mounting of storage rack has the culture dish, the inside wall fixed mounting who holds the box has the top and the culture dish is located the ultraviolet lamp in the coplanar, the bottom intercommunication that holds the box has the heating pipe of bottom and cell culture jar's inner chamber diapire intercommunication, the fan groove with the heating pipe intercommunication is seted up to the inside of cell culture jar, the bottom intercommunication of cell culture jar has the air-supply line with fan groove intercommunication, but this design temperature regulation degree of automation is low.

Disclosure of Invention

In view of the drawbacks of the prior art, it is an object of the present invention to provide a cell culture apparatus for observation.

The cell culture device for observation provided by the invention comprises a culture dish main body, a culture dish adapter plate, a water bath cover plate and a sliding cover;

the culture dish main body comprises a shell and a vertical plate which is arranged on the inner side of the shell and connected with the shell, wherein a first accommodating space is formed between the outer side surface of the vertical plate and the shell, and a second accommodating space is formed by the inner side surface of the vertical plate and the part of the shell in a surrounding manner;

the culture dish adapter plate can be installed in the second accommodating space in a matched mode and supported by the edge of the vertical plate extending towards the inner side through the bottom end of the vertical plate, and the culture dish adapter plate is used for installing a culture dish;

the top ends of the two sides of the shell are respectively provided with oppositely arranged sliding grooves, the two sides of the sliding cover can be slidably arranged in the two sliding grooves, and the water bath cover plate is arranged at the top end of the vertical plate and is arranged below the sliding cover;

the sliding cover is provided with two states of opening and closing, the heating glass is arranged on the sliding cover, when the sliding cover is in the closing state, the heating glass is in the power-on state, and when the sliding cover is in the opening state, the heating glass is in the power-off state.

Preferably, the end part of the sliding cover is provided with a magnetic attraction structure, and the shell is provided with a structure contact matched with the magnetic attraction structure;

when the sliding cover is in a closed state, the magnetic attraction structure is connected with the structure contact.

Preferably, the device further comprises a control mechanism, a first temperature sensor, a second temperature sensor and a heater;

the first accommodating space is used for accommodating water, the heater is arranged outside the shell and faces the first accommodating space, and the first temperature sensor is arranged in the first accommodating space;

the first temperature sensor, the second temperature sensor, the heater and the heating glass are respectively electrically connected with the control mechanism.

Preferably, the control mechanism is capable of controlling the heater and the heating glass electrifying current according to a preset target temperature interval and temperature information obtained from the first temperature sensor and the second temperature sensor.

Preferably, the first accommodating space is a U-shaped space and surrounds the outside of the second accommodating space;

the shell is provided with a first flow passage communicated with the U-shaped space, wherein the first flow passage is used for supplying air.

Preferably, a first valve is connected to one end of the first flow passage outside the housing, and the control mechanism is in signal connection with the first valve.

Preferably, an observation window is arranged on the shell and used for observing the height of the liquid level in the first accommodating space.

Preferably, the culture dish comprises a culture dish body, wherein the culture dish body can be matched and installed in the C-shaped space of the microscope adapter bottom plate and is supported by the bottom plate edge on the inner side of the microscope adapter bottom plate.

Preferably, one or more gaps arranged at intervals are formed in the edge of the top end of the vertical plate, so that the first accommodating space is communicated with the second accommodating space through the gaps when the water bath cover plate covers the top end of the vertical plate.

Preferably, the culture dish adapter plate comprises a single well as a multi-well structure.

Compared with the prior art, the invention has the following beneficial effects:

1. according to the invention, the on-off of the heating glass is realized by the contact mode through sliding, so that the culture dish is subjected to radiation heating, the stable sample temperature can be kept corresponding to the change of the external environment, the structure is simple, and the practicability is strong.

2. The control mechanism can control the current of the heater and the heating glass according to the preset target temperature interval and the temperature information obtained from each temperature sensor, so as to stably and automatically control the ambient temperature, and the temperature can be controlled within +/-0.1 ℃ of the target temperature, thereby improving the accuracy of temperature control.

3. The invention realizes the contact of the conductive contact by adopting the magnetic attraction structure of the structure contact and the magnetic attraction structure, realizes the wireless power supply of the slide cover to the heating glass, and simultaneously solves the problem that the microscope structure limits the height of the flip cover.

4. The invention solves the problem of adapting to culture dishes with different specifications by designing the structure of the adapting plate of the culture dish, and has good universality.

5. The microscope adapter bottom plate structure is adopted, so that the microscope adapter bottom plate structure can be matched with a microscope for observation, and the use is convenient.

6. The invention adopts a water tank heating type heating structure and a relatively closed space as a whole, so that the temperature and the humidity in the culture dish are more stable.

Drawings

Other features, objects and advantages of the invention will become more apparent upon reading of the detailed description of non-limiting embodiments with reference to the following drawings:

FIG. 1 is a schematic view of the overall structure of the present invention, wherein one side of the sliding cover is shown;

FIG. 2 is a schematic view of the overall structure of the present invention, wherein one side of a single well plate adapter plate is shown;

FIG. 3 is a schematic view of the structure of the main body of the culture dish;

FIG. 4 is a schematic diagram of the structure of a 96-well plate;

FIG. 5 is a schematic view of a slider;

FIG. 6 is a schematic structural view of a microscope adapter base plate;

FIG. 7 is a schematic view of the water bath cover plate;

FIG. 8 is a schematic structural diagram of a D60 single-well plate;

FIG. 9 is a schematic structural diagram of a D35 double-well plate adapter;

fig. 10 is a schematic view of a structure contact.

The figures show that:

culture dish main body 1 casing 101

2 chutes 1011 of culture dish adapter plate

Water bath cover plate 3 structure contact 1012

Slide cover 4 power contact 1013

Magnetic attraction structure 41 vertical plate 102

First accommodation space 103 of metal conductive contact 42

Second accommodating space 104 of heating glass 5

Microscope adapter base plate 6 riser edge 105

Floor edge 61 first flow channel 106

Gap 7 second flow channel 107

Observation window 108 of sensor mounting hole 8

Detailed Description

The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that it would be obvious to those skilled in the art that various changes and modifications can be made without departing from the spirit of the invention. All falling within the scope of the present invention.

The invention provides a cell culture device for observation, which comprises a culture dish main body 1, a culture dish adapter plate 2, a water bath cover plate 3 and a sliding cover 4, as shown in figures 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10, the culture dish body 1 comprises a housing 101 and a vertical plate 102 arranged inside the housing 101 and connected to the housing 101, the vertical plate 102 is preferably integrally connected to the housing 101, wherein a first accommodating space 103 is formed between the outer side surface of the vertical plate 102 and the shell 101, the first accommodating space 103 is used for accommodating water, the inner side surface of the vertical plate 102 and a part of the shell 101 enclose a second accommodating space 104, the culture dish adapter plate 2 can be fittingly installed in the second accommodating space 104 and is supported by a vertical plate edge 105 extending towards the inner side from the bottom end of the vertical plate 102, and the culture dish adapter plate 2 is used for installing a culture dish.

The top ends of the two sides of the housing 101 are respectively provided with sliding grooves 1011 which are oppositely arranged, the two sides of the sliding cover 4 are slidably arranged in the two sliding grooves 1011, the water bath cover plate 3 is arranged at the top end of the vertical plate 102 and arranged below the sliding cover 4, a certain distance is arranged between the top end of the vertical plate 102 and the sliding cover 4, the distance is larger than the thickness of the water bath cover plate 3 so as to allow the water bath cover plate 3 to be covered on the upper part of the vertical plate 102, the edge of the top end of the vertical plate 102 is provided with one or more notches 7 which are arranged at intervals so as to enable the first accommodating space 103 to be communicated with the second accommodating space 104 through the notch 7 when the water bath cover plate 3 is covered on the top end of the vertical plate 102, and water vapor generated by heating the water in the first accommodating space 103 can enter the second accommodating space 104 through the notch 7 so as to ensure that the environment inside the second accommodating space 104 is kept warm and humidified, thereby providing a suitable external environment for the cells.

The sliding cover 4 has two states of opening and closing, the heating glass 5 is arranged on the sliding cover 4, when the sliding cover 4 is in the closing state, the heating glass 1 is in the power-on state, and when the sliding cover 4 is in the opening state, the heating glass 1 is in the power-off state. In a preferred embodiment, the housing 101 is provided with a structure contact 1012, the end portion of the sliding cover 4 is provided with a magnetic attraction structure 41 adapted to the structure contact 1012, as shown in fig. 5 and 10, when the sliding cover 4 is in a closed state, the magnetic attraction structure 41 can be magnetically attracted to the structure contact 1012, the middle portion of the magnetic attraction structure 41 is provided with a metal conductive contact 42, the metal conductive contact 42 is in contact communication with the power contact 1013 on the structure contact 1012 to achieve electric conduction of the heating glass 1, the magnetic attraction structure 41 and the structure contact 1012 preferably include permanent magnet materials, and the two opposite sides of the two structures have opposite magnetism and thus can achieve magnetic attraction.

The water heater also comprises a control mechanism, a first temperature sensor, a second temperature sensor and a heater, wherein the heater is arranged on the bottom end face outside the shell 101 and is opposite to the first accommodating space 103 positioned inside the shell 101, the control mechanism controls the heater to heat, heat is transferred to the first accommodating space 103 through the shell 101 so as to heat water in the first accommodating space 103, heat preservation and humidification of the environment are realized, the heater is preferably an electric heating wire, the space can be saved, and the heating efficiency is high.

Further, as shown in fig. 3, the first temperature sensor is disposed in the first receiving space 103, the second temperature sensor is disposed in the sensor mounting hole 8, the first temperature sensor accurately reflects the temperature of water, the second temperature sensor can reflect the temperature of the solution in the culture dish, and the cables connected to the first temperature sensor and the second temperature sensor preferably transmit the temperature detection signal through the aviation plug mounted on the housing 101. First temperature sensor, second temperature sensor, heater, heating glass 5 respectively with control mechanism electricity is connected, control mechanism can be according to the interval of predetermined target temperature and follow the temperature information control that first temperature sensor, second temperature sensor obtained heater, 5 circular telegram electric current's of heating glass size, and then realize the regulation and control to the regional temperature of culture dish, and then make temperature control at the target temperature interval. The invention can set different target temperature intervals aiming at culture dishes with different heights so as to realize stable temperature regulation and control, thereby meeting the requirements of actual products.

The first accommodating space 103 is a U-shaped space and surrounds the outside of the second accommodating space 104, so that heated water surrounds the culture dish and is easier to transfer temperature. The housing 101 has a first flow channel 106 and a second flow channel 107 which are communicated with the U-shaped space, wherein the first flow channel 106 is used for supplying gas to ensure the required gas in the second accommodating space 104, the gas is discharged at the gap position of the device, and the second flow channel 107 is used for filling cells in the culture dish. In practical applications, the number of the second flow channels 107 is preferably two, one is in and one is out, and the two flow channels are preferably powered by the peristaltic pump, and the control mechanism can control the start and stop of the peristaltic pump and the specific start and stop time, and can be set in the system, so that the replacement of the internal cells can be realized without opening the sliding cover 4. The housing 101 is further provided with an overflow port through which water in the first receiving space 103 can flow out after exceeding a certain level.

The first flow channel 106 is connected to a first valve at one end outside the housing 101, and the control mechanism is in signal connection with the first valve. The control mechanism controls the opening of the first valve to realize that gas with different concentrations is introduced into the shell 101 to achieve a cell culture environment, and the gas concentration can be controlled to be +/-0.2%. The water can be directly added by opening the sliding cover 4.

An observation window 108 is arranged on the shell 101, and scales are arranged on the observation window 108 and used for observing the height of the liquid level in the first accommodating space 103.

The culture dish comprises a microscope adapter bottom plate 6, wherein the microscope adapter bottom plate 6 is of a C-shaped structure as a whole, and as shown in FIG. 6, the culture dish body 1 can be installed in the C-shaped space of the microscope adapter bottom plate 6 in a matching mode and is supported by a bottom plate edge 61 on the inner side of the microscope adapter bottom plate 6. Microscope adapter bottom plate 6 can be convenient let extension observe on the microscope, has improved the convenience of using.

The culture dish adapting plate 2 comprises a single-hole and multi-hole structure, and can be matched with different culture dishes, such as a D60 single hole, and a D35 single hole or double holes, so that the universality of the product is greatly improved.

In the description of the present application, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience in describing the present application and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present application.

The foregoing description of specific embodiments of the present invention has been presented. It is to be understood that the present invention is not limited to the specific embodiments described above, and that various changes or modifications may be made by one skilled in the art within the scope of the appended claims without departing from the spirit of the invention. The embodiments and features of the embodiments of the present application may be combined with each other arbitrarily without conflict.