阅读说明：本技术 一种制备人工软骨的方法 (Method for preparing artificial cartilage ) 是由 傅利锋 傅锦汀 王政 于 2021-09-17 设计创作，主要内容包括：本发明公开了一种制备人工软骨的方法,包括：在搅拌下向聚乙烯醇溶液中加入聚乙二醇溶液形成白色凝胶；对白色凝胶进行离心处理；将离心处理后的白色凝胶加入有机溶剂中继续离心处理,干燥后得到聚乙烯醇/聚乙二醇人工软骨。以聚乙烯醇溶液与聚乙二醇溶液为原料得到凝胶,并通过离心作用增加聚乙二醇溶液的渗透压提高交联度,然后将凝胶放入有机溶剂中离心脱除水分,能改善凝胶的力学性能,提高人工软骨的强度。(The invention discloses a method for preparing artificial cartilage, which comprises the following steps: adding polyethylene glycol solution into polyvinyl alcohol solution under stirring to form white gel; centrifuging the white gel; and adding the white gel after the centrifugal treatment into an organic solvent for continuous centrifugal treatment, and drying to obtain the polyvinyl alcohol/polyethylene glycol artificial cartilage. The gel is obtained by taking the polyvinyl alcohol solution and the polyethylene glycol solution as raw materials, the osmotic pressure of the polyethylene glycol solution is increased through the centrifugal action to improve the crosslinking degree, and then the gel is put into an organic solvent to centrifugally remove water, so that the mechanical property of the gel can be improved, and the strength of the artificial cartilage is improved.)

1. A method of preparing artificial cartilage, comprising the steps of:

step 1, adding a polyethylene glycol solution into a polyvinyl alcohol solution under stirring to form white gel;

step 2, carrying out centrifugal treatment on the white gel;

and 3, adding the centrifuged white gel into an organic solvent, continuously centrifuging, and drying to obtain the polyvinyl alcohol/polyethylene glycol artificial cartilage.

2. The method for preparing artificial cartilage according to claim 1, wherein the mass fraction of the polyvinyl alcohol solution in step 1 is 5% -15%, the mass fraction of the polyethylene glycol solution is 15% -45%, and the volume ratio of the polyvinyl alcohol to the polyethylene glycol solution is 3-10: 1.

3. the method as claimed in claim 1, wherein the stirring speed in step 1 is 200-400r/min and the stirring time is 5-10 min.

4. The method for preparing artificial cartilage according to claim 1, wherein the specific process in step 3 is as follows: and adding the centrifuged white gel into an organic solvent for centrifuging for multiple times, replacing the organic solvent after each centrifuging, and drying to obtain the polyvinyl alcohol/polyethylene glycol artificial cartilage.

5. The method for preparing artificial cartilage according to claim 4, wherein the parameters of the centrifugation in the steps 2-3 are: the rotating speed is 2000-4000r/min, and the centrifugation time is 5-30 min.

6. The method for preparing artificial cartilage according to claim 1, wherein the organic solvent in step 3 is one or more of ethanol, acetone and isopropanol.

7. The method for preparing artificial cartilage according to claim 1, wherein the drying temperature in the step 3 is 25-60 ℃.

Technical Field

The invention belongs to the technical field of medical materials, and relates to a method for preparing artificial cartilage.

Background

Hydrogels have received much attention from researchers due to their good applications in sensors, tissue engineering, drug delivery, and superabsorbent materials. In addition, the hydrogel is used as a hydrophilic polymer chain network, and a fluid interface layer formed by a liquid film permeating from the surface of the hydrogel and a solvated flexible polymer can play a good lubricating effect.

The polyvinyl alcohol hydrogel has a pore structure similar to that of cartilage, has good hydrophilicity, excellent biocompatibility and tribological performance, and becomes an ideal material for artificial cartilage. Natural cartilage has greater toughness and a unique fluid-pressurized structure, as compared to which pure polyvinyl alcohol gel lacks sufficient strength.

Disclosure of Invention

The invention aims to provide a method for preparing artificial cartilage, which solves the problem of low gel strength of pure polyvinyl alcohol in the prior art.

The technical scheme adopted by the invention is that the method for preparing the artificial cartilage comprises the following steps:

step 1, adding a polyethylene glycol solution into a polyvinyl alcohol solution under stirring to form white gel;

step 2, carrying out centrifugal treatment on the white gel;

and 3, adding the centrifuged white gel into an organic solvent, continuously centrifuging, and drying to obtain the polyvinyl alcohol/polyethylene glycol artificial cartilage.

The invention is also characterized in that:

in the step 1, the mass fraction of the polyvinyl alcohol solution is 5-15%, the mass fraction of the polyethylene glycol solution is 15-45%, and the volume ratio of the polyvinyl alcohol to the polyethylene glycol solution is 3-10: 1.

the stirring speed in the step 1 is 200-400r/min, and the stirring time is 5-10 min.

The specific process of the step 3 is as follows: and adding the centrifuged white gel into an organic solvent for centrifuging for multiple times, replacing the organic solvent after each centrifuging, and drying to obtain the polyvinyl alcohol/polyethylene glycol artificial cartilage.

The parameters of the centrifugal treatment in the step 2-3 are as follows: the rotating speed is 2000-4000r/min, and the centrifugation time is 5-30 min.

The organic solvent in the step 3 is one or a combination of ethanol, acetone and isopropanol.

The drying temperature in step 3 is 25-60 ℃.

The invention has the beneficial effects that:

the invention relates to a method for preparing artificial cartilage, which takes a polyvinyl alcohol solution and a polyethylene glycol solution as raw materials to obtain gel, increases the osmotic pressure of the polyethylene glycol solution by centrifugation to improve the crosslinking degree, and then puts the gel into an organic solvent to remove water by centrifugation, thereby improving the mechanical property of the gel and improving the strength of the artificial cartilage.

Drawings

FIG. 1 is a graph showing the results of a friction test according to an embodiment of a method for preparing artificial cartilage according to the present invention;

FIG. 2 is a graph showing the results of hardness test according to an example of a method for preparing artificial cartilage according to the present invention.

Detailed Description

The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.

A method of preparing artificial cartilage comprising the steps of:

step 1, adding 15-45 mass percent of polyethylene glycol solution into 5-15 mass percent of polyvinyl alcohol solution under stirring to form white bulk gel, wherein the stirring speed is 200-400r/min, the stirring time is 5-10min, and the volume ratio of the polyvinyl alcohol to the polyethylene glycol solution is 3-10: 1;

step 2, carrying out centrifugal treatment on the white bulk gel at the rotating speed of 2000-4000r/min for 5-30min so as to further carry out permeation crosslinking under the action of centrifugal force;

step 3, adding the centrifuged white gel into an organic solvent for continuous centrifugation for 2-6 times, replacing the organic solvent after each centrifugation, wherein the rotation speed is 2000-4000r/min and the time is 5-30min each time, and then drying at 25-60 ℃ to obtain the polyvinyl alcohol/polyethylene glycol artificial cartilage; the organic solvent is one or more of ethanol, acetone and isopropanol.

The principle of the method for preparing the artificial cartilage is as follows: adding the polyvinyl alcohol solution into the polyethylene glycol solution which is continuously stirred, wherein the stirring process is used for promoting the crosslinking of the two solutions. However, the formed gel is not sufficiently cross-linked because the cross-linking of the surface prevents the solution of polyethylene glycol from permeating, and the centrifugal force of the centrifuge can increase the osmotic pressure of the polyethylene glycol solution to increase the degree of cross-linking. The crosslinked gel network contains a large amount of water, a layer of film can be formed on the surface of the gel to influence the volatilization of the water in the gel by common drying, the gel is put into an organic solvent for centrifugation, and the water in the gel can be effectively removed by carrying out multiple times of centrifugation treatment by using the organic solvent. The network structure of the gel can be adjusted by extracting water in the gel with an organic solvent.

According to the mode, the method for preparing the artificial cartilage comprises the steps of taking a polyvinyl alcohol solution and a polyethylene glycol solution as raw materials to obtain gel, increasing osmotic pressure of the polyethylene glycol solution through a centrifugal effect to improve the crosslinking degree, and then putting the gel into an organic solvent to remove water through centrifugation, so that the mechanical property of the gel can be improved, and the strength of the artificial cartilage is improved.

Example 1

Adding 10% by mass of polyvinyl alcohol solution into 30% by mass of polyethylene glycol solution under stirring, fully stirring at the stirring speed of 300 r/min for 8 min to form white gel, and centrifuging the white gel mixed solution at the rotating speed of 3000 r/min for 10 min. Removing water solution from the centrifuged gel, adding ethanol, continuously rotating at 4000 rpm for 20 min each time, and repeating for 2 times after ethanol replacement. Drying at 25 deg.C after centrifugation to obtain polyvinyl alcohol/polyethylene glycol artificial cartilage.

The artificial cartilage obtained in this example was subjected to a reciprocating friction test using a friction tester under dry friction at a fixed speed of 1 cm/sec and a fixed load of 5 newtons, and the gel surface was subjected to a hardness test using a shore durometer, which was repeated three times for each test, and the average value was taken for analysis. The error line is obtained by the standard deviation of the error line, the friction test results and the hardness test results are respectively shown in figure 1 and figure 2, the polyvinyl alcohol/polyethylene glycol artificial cartilage after centrifugal treatment by three organic solvents has low average friction coefficient, and compared with pure polyvinyl alcohol hydrogel, the hardness of the artificial cartilage is improved by 177.4%, and the artificial cartilage has excellent mechanical properties.

Example 2

Adding a polyethylene glycol solution with the mass fraction of 15% into a polyvinyl alcohol solution with the mass fraction of 5% under stirring, fully stirring at the stirring speed of 400r/min for 8 min to form white gel, and centrifuging the white gel mixed solution at the rotating speed of 3000 r/min for 20 min. Removing the water solution from the centrifuged gel, adding acetone, continuously rotating at 4000 rpm for 10 minutes each time, and repeating the process for 3 times after replacing acetone. Drying at 60 deg.C after centrifugation to obtain polyvinyl alcohol/polyethylene glycol artificial cartilage.

Example 3

Adding 15% by mass of polyvinyl alcohol solution into 30% by mass of polyethylene glycol solution under stirring, fully stirring at the stirring speed of 300 r/min for 8 min to form white gel, and centrifuging the white gel mixed solution at the rotating speed of 2000 r/min for 15 min. Removing the water solution from the centrifuged gel, adding acetone, continuing to rotate at the speed of 4000 revolutions per minute for 30 minutes each time, and repeating the process for 2 times after the acetone is replaced by isopropanol. Drying at 25 deg.C after centrifugation to obtain polyvinyl alcohol/polyethylene glycol artificial cartilage.

Example 4

Adding 10% by mass of polyvinyl alcohol solution into 45% by mass of polyethylene glycol solution under stirring, fully stirring at the stirring speed of 300 r/min for 8 min to form white gel, and centrifuging the white gel mixed solution at the rotation speed of 4000r/min for 30 min. Removing water solution from the centrifuged gel, adding isopropanol, continuously rotating at 4000 rpm for 20 min each time, and repeating for 2 times after isopropanol is replaced. Drying at 50 deg.C after centrifugation to obtain polyvinyl alcohol/polyethylene glycol artificial cartilage.

Example 5

Adding 10% by mass of polyvinyl alcohol solution into 30% by mass of polyethylene glycol solution under stirring, fully stirring at the stirring speed of 300 r/min for 8 min to form white gel, and centrifuging the white gel mixed solution at the rotating speed of 3000 r/min for 20 min. Removing the water solution from the centrifuged gel, adding acetone, continuing to rotate at the speed of 4000 revolutions per minute, centrifuging for 30 minutes each time, and repeating for 4 times after replacing the acetone. Drying at 40 deg.C after centrifugation to obtain polyvinyl alcohol/polyethylene glycol artificial cartilage.