阅读说明：本技术 一种子宫宫膜修复凝胶 (Uterine endometrium repair gel ) 是由 徐丽君 张可研 杨维 于 2021-08-13 设计创作，主要内容包括：本发明提供一种子宫宫膜修复凝胶,涉及修复凝胶领域。该一种子宫宫膜修复凝胶,包括以下组分,按重量计算：磷酸和磷酸氢二钠所组成的缓冲液15～25份、壳寡糖纳米银1～5份、甘油3～10份、脂氧素0.1～0.2份、维生素C 0.2～0.5份、维生素B-20.1～0.3份,所述修复凝胶的制备方向如下：在室温常压下向缓冲液中依次加入甘油、维生素C、维生素B-2、脂氧素均匀搅拌,再加入壳寡糖纳米银后充分搅拌后获得修复凝胶。通过合理搭配,获得对宫膜修复效果好的凝胶。(The invention provides a uterine endometrium repair gel, and relates to the field of repair gels. The uterine endometrium repair gel comprises the following components in parts by weight: 15-25 parts of buffer solution consisting of phosphoric acid and disodium hydrogen phosphate, 1-5 parts of chitosan oligosaccharide nano-silver, 3-10 parts of glycerol, 0.1-0.2 part of lipoxin, 0.2-0.5 part of vitamin C, and vitamin B 2 0.1-0.3 parts of repairing gel, wherein the preparation direction of the repairing gel is as follows: sequentially adding glycerol, vitamin C and vitamin B into buffer solution at room temperature and normal pressure 2 And lipoxin are evenly stirred, and then chitosan oligosaccharide nano silver is added and fully stirred to obtain the repairing gel. By reasonable matching, the gel with good effect of repairing the uterine membrane is obtained.)

1. The uterine endometrium repair gel is characterized by comprising the following components in parts by weight: 15-25 parts of buffer solution consisting of phosphoric acid and disodium hydrogen phosphate, 1-5 parts of chitosan oligosaccharide nano-silver, 3-10 parts of glycerol, 0.1-0.2 part of lipoxin, 0.2-0.5 part of vitamin C, and vitamin B₂0.1-0.3 parts of repairing gel, wherein the preparation direction of the repairing gel is as follows:

sequentially adding glycerol, vitamin C and vitamin B into buffer solution at room temperature and normal pressure₂And lipoxin are evenly stirred, and then chitosan oligosaccharide nano silver is added and fully stirred to obtain the repairing gel.

2. The uterine membrane repair gel according to claim 1, wherein: the buffer solution is prepared according to the content of phosphoric acid and disodium hydrogen phosphate, so that the pH value of the buffer solution is 7-8.

3. The uterine membrane repair gel according to claim 1, wherein: the relative molecular mass of the chitosan oligosaccharide is 2000-3000 Da.

4. The uterine membrane repair gel according to claim 1, wherein: the buffer configurations shown are as follows: adding deionized water into a preparation container, then adding phosphoric acid and disodium hydrogen phosphate, detecting the pH of the solution by using test paper after the solution is recovered to normal temperature, then adding phosphoric acid and disodium hydrogen phosphate, and filtering after meeting the required pH, wherein the obtained filtrate is a buffer solution for later use.

5. The uterine membrane repair gel according to claim 1, wherein: the chitosan oligosaccharide nano-silver is prepared as follows: 1) crosslinking chitosan oligosaccharide and lipoic acid by using EDC [1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride ] and NHS (N-hydroxysuccinimide) to obtain a chitosan oligosaccharide lipoic acid polymer, firstly preparing a 5% chitosan oligosaccharide aqueous solution, then adding 1% EDC and 0.5% NHS, dissolving 1% lipoic acid in 15 times of absolute ethyl alcohol by volume ratio until the mixture is completely dissolved, then adding the solution, stirring in a water bath at 40 ℃ for 24 hours, placing the mixture in a molecular weight cut-off 2000Da dialysis bag after stirring, dialyzing with deionized water for 3 days, changing water once every 12 hours, adding absolute ethyl alcohol into a dialysate retentate, carrying out rotary evaporation, and then freezing the dried chitosan oligosaccharide lipoic acid polymer; 2) preparing a nano silver solution, namely mixing 10 parts of 1mmol/L silver nitrate solution and 2 parts of 401mmol/L sodium citrate solution, slowly dripping the mixture into 30 parts of 10mmol/L sodium borohydride solution, quickly stirring for 30min, and aging for 2h to obtain the nano silver solution; 3) preparing the freeze-dried chitosan oligosaccharide lipoic acid polymer into 2mg/mL aqueous solution, slowly dripping 20 parts of chitosan oligosaccharide lipoic acid polymer solution into the nano-silver solution prepared in the last step, quickly stirring, putting into a dialysis bag with the molecular weight cutoff of 10000Da for dialysis, and finally freeze-drying to obtain the chitosan oligosaccharide modified nano-silver.

Technical Field

The invention relates to the technical field of repair gel, in particular to uterine endometrium repair gel.

Background

The uterus is one of female internal genital organs, is a cavity organ, is positioned in the center of a pelvic cavity, is in an inverted pear shape, and is slightly flat in the front and slightly protruded in the back. The uterus may have diseases such as cervicitis, cervical tumor, endometriosis, adenomyosis and uterine dysplasia. Especially after the uterine curettage, the damage to the uterus is large, and the uterus membrane is quickly repaired by the medicine.

The uterus is in vivo and, when drugs are used for repair, the drugs are basically injected into the uterus. The injected drug does not work effectively due to the rejection of the body. Therefore, the conventional medicament selects the endometrial acellular matrix of the same animal to reduce the degree of rejection of the body. For example, the Chinese patent discloses a hydrogel for repairing endometrial injury and a preparation method thereof, wherein the application number is '201811299403.5', the hydrogel improves the repairing effect of uterine endometrium by using an endometrial acellular matrix of the same animal, the mode is difficult in selecting raw materials, meanwhile, the endometrial acellular matrix has certain activity, and the storage time of the drug is limited.

And the pH value of the vagina is generally less than 4.5, and the drug performance can be influenced by the external pH value when the vagina is administrated, so that the drug effect is greatly reduced.

Disclosure of Invention

Technical problem to be solved

Aiming at the defects of the prior art, the invention provides a uterus endometrium repair gel which is a medicine with good uterus endometrium repair effect and easily obtained raw materials.

(II) technical scheme

In order to achieve the purpose, the invention is realized by the following technical scheme: a uterus endometrium repair gel comprises the following components by weight: 15-25 parts of buffer solution consisting of phosphoric acid and disodium hydrogen phosphate, 1-5 parts of chitosan oligosaccharide nano-silver, 3-10 parts of glycerol, 0.1-0.2 part of lipoxin, 0.2-0.5 part of vitamin C, and vitamin B₂0.1-0.3 parts of repairing gel, wherein the preparation direction of the repairing gel is as follows:

sequentially adding glycerol, vitamin C and vitamin B into buffer solution at room temperature and normal pressure₂And lipoxin are evenly stirred, and then chitosan oligosaccharide nano silver is added and fully mixed to obtain the repairing gel.

Preferably, the buffer solution is prepared according to the content of the phosphoric acid and the disodium hydrogen phosphate, so that the pH value of the buffer solution is 7-8.

Preferably, the relative molecular mass of the chitosan oligosaccharide is 2000-3000 Da.

Preferably, the buffer is prepared as follows: adding deionized water into a preparation container, then adding phosphoric acid and disodium hydrogen phosphate, detecting the pH of the solution by using test paper after the solution is recovered to normal temperature, then adding phosphoric acid and disodium hydrogen phosphate, and filtering after meeting the required pH, wherein the obtained filtrate is a buffer solution for later use.

Preferably, the chitosan oligosaccharide nano silver is prepared as follows: 1) crosslinking chitosan oligosaccharide and lipoic acid by using EDC [1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride ] and NHS (N-hydroxysuccinimide) to obtain a chitosan oligosaccharide lipoic acid polymer, firstly preparing a 5% chitosan oligosaccharide aqueous solution, then adding 1% EDC and 0.5% NHS, dissolving 1% lipoic acid in 15 times of absolute ethyl alcohol by volume ratio until the mixture is completely dissolved, then adding the solution, stirring in a water bath at 40 ℃ for 24 hours, placing the mixture in a molecular weight cut-off 2000Da dialysis bag after stirring, dialyzing with deionized water for 3 days, changing water once every 12 hours, adding absolute ethyl alcohol into a dialysate retentate, carrying out rotary evaporation, and then freezing the dried chitosan oligosaccharide lipoic acid polymer; 2) preparing a nano silver solution, namely mixing 10 parts of 1mmol/L silver nitrate solution and 2 parts of 401mmol/L sodium citrate solution, slowly dripping the mixture into 30 parts of 10mmol/L sodium borohydride solution, quickly stirring for 30min, and aging for 2h to obtain the nano silver solution; 3) preparing the freeze-dried chitosan oligosaccharide lipoic acid polymer into 2mg/mL aqueous solution, slowly dripping 20 parts of chitosan oligosaccharide lipoic acid polymer solution into the nano-silver solution prepared in the last step, quickly stirring, putting into a dialysis bag with the molecular weight cutoff of 10000Da for dialysis, and finally freeze-drying to obtain the chitosan oligosaccharide modified nano-silver.

(III) advantageous effects

The invention provides a uterine endometrium repair gel. The method has the following beneficial effects:

1. the buffer solution is added to ensure the consistency of the medicine and the uterine cavity environment, and the buffer system reduces the influence of the vaginal environment on the medicine during administration, so that the medicine has long-acting effect and the uterine membrane repair effect is improved.

2. According to the invention, the chitosan oligosaccharide lipoic acid polymer is used for modifying the nano-silver, so that the stability of the nano-silver is increased, the antibacterial effect is improved, and the uterine inflammation can be repaired.

3. According to the invention, the uterine membrane repair is realized through biological activities of chitosan oligosaccharide molecules, such as cell damage repair, immunity enhancement, free radical removal and the like.

4. The materials in the ratio are easy to obtain.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The first embodiment is as follows:

the embodiment of the invention provides a uterine endometrium repair gel which comprises the following components in parts by weight: 15 parts of buffer solution consisting of phosphoric acid and disodium hydrogen phosphate, wherein the buffer solution is matched with the contents of the phosphoric acid and the disodium hydrogen phosphate, so that the pH range of the buffer solution is 7-8, the pH value of cervical secretion in the middle of a physiological cycle is 7-8.5, and the consistency of the medicine and a body fluid environment is ensured. In addition, the pH value of the normal vagina is less than 4.5, and a buffer solution is prepared, so that the gel is not influenced by the acidic environment of the vagina when being administered, the relatively stable pH value can be kept for a long time, and the effect of the gel effect is improved;

2 parts of chitosan oligosaccharide nano silver. The nano silver has strong and durable antibacterial property, and is easy to agglomerate and settle to influence the antibacterial stability due to small particle size and large specific surface area. The chitosan oligosaccharide is oligosaccharide with the polymerization degree of 2-20, which is obtained by performing biological enzymolysis or chemical degradation on chitosan, has biological activities of resisting oxidation, protecting cells, enhancing immunity and the like, and has good water solubility and easy preparation and absorption by a human body. Chitosan oligosaccharide also has certain bacteriostatic and anti-inflammatory effects, but is more limited. The chitosan oligosaccharide lipoic acid polymer with good stability is obtained by grafting lipoic acid with chitosan oligosaccharide, then covalent chelation is carried out on the surface of nano-silver by disulfide bonds in the chitosan oligosaccharide lipoic acid polymer, and surface modification is carried out on nano-silver particles, so that the chitosan oligosaccharide nano-silver with stable performance is obtained. The chitosan oligosaccharide nano silver has the advantages that the antibacterial property of the nano silver and the antibacterial property of the chitosan oligosaccharide are cooperated, so that the antibacterial efficiency is improved, and meanwhile, the biological activity of the chitosan oligosaccharide is introduced;

5 parts of glycerol, wherein the glycerol has no toxicity to human bodies, can improve the penetration capacity of the medicine to the skin, improve the medicine effect, promote the dissolution of vitamins and the like, has good lubricating capacity, and can discharge the repairing gel from the human body and promote the discharge of damaged tissues from the human body if the human body has rejection;

0.3 part of lipoxin, wherein in the existing research, the lipoxin has the function of treating inflammatory or autoimmune diseases, and can reduce the inflammation probability in repairing the damage of uterine membranes;

0.4 part of vitamin C, wherein the vitamin C is necessary for antibody and collagen formation, tissue repair (including certain redox actions), immune function maintenance, vessel integrity maintenance, non-heme iron absorption promotion and the like, and can improve the wound repair efficiency;

vitamin B₂0.1 part, vitamin B2 has the main physiological function of acting as coenzyme promoterCan promote metabolism and has anti-inflammatory effect.

The preparation direction of the repairing gel is as follows:

the buffer was prepared as follows: adding deionized water into a preparation container, then adding phosphoric acid and disodium hydrogen phosphate, detecting the pH of the solution by using test paper after the solution is recovered to normal temperature, then adding phosphoric acid and disodium hydrogen phosphate, and filtering after meeting the required pH, wherein the obtained filtrate is a buffer solution for later use;

the chitosan oligosaccharide nano silver is prepared as follows: 1) crosslinking chitosan oligosaccharide and lipoic acid by using EDC [1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride ] and NHS (N-hydroxysuccinimide) to obtain a chitosan oligosaccharide lipoic acid polymer, firstly preparing a 5% chitosan oligosaccharide aqueous solution, then adding 1% EDC and 0.5% NHS, dissolving 1% lipoic acid in 15 times of absolute ethyl alcohol by volume ratio until the mixture is completely dissolved, then adding the solution, stirring in a water bath at 40 ℃ for 24 hours, placing the mixture in a molecular weight cut-off 2000Da dialysis bag after stirring, dialyzing with deionized water for 3 days, changing water once every 12 hours, adding absolute ethyl alcohol into a dialysate retentate, carrying out rotary evaporation, and then freezing the dried chitosan oligosaccharide lipoic acid polymer; 2) preparing a nano silver solution, namely mixing 10 parts of 1mmol/L silver nitrate solution and 2 parts of 401mmol/L sodium citrate solution, slowly dripping the mixture into 30 parts of 10mmol/L sodium borohydride solution, quickly stirring for 30min, and aging for 2h to obtain the nano silver solution; 3) preparing the freeze-dried chitosan oligosaccharide lipoic acid polymer into 2mg/mL aqueous solution, slowly dripping 20 parts of chitosan oligosaccharide lipoic acid polymer solution into the nano-silver solution prepared in the last step, quickly stirring, putting into a dialysis bag with the molecular weight cutoff of 10000Da for dialysis, and finally freeze-drying to obtain the chitosan oligosaccharide modified nano-silver.

Sequentially adding glycerol, vitamin C and vitamin B into buffer solution at room temperature and normal pressure₂And lipoxin is uniformly stirred, and then chitosan oligosaccharide nano silver is added and fully stirred to obtain the repairing gel.

The buffer solution is prepared according to the content of the phosphoric acid and the disodium hydrogen phosphate, so that the pH value of the buffer solution is 7-8.

Example two:

the embodiment of the invention provides a uterine endometrium repair gel which comprises the following components in parts by weight: 20 parts of buffer solution consisting of phosphoric acid and disodium hydrogen phosphate, wherein the buffer solution is matched with the contents of the phosphoric acid and the disodium hydrogen phosphate, so that the pH range of the buffer solution is 7-8, the pH value of cervical secretion in the middle of a physiological cycle is 7-8.5, and the consistency of the medicine and a body fluid environment is ensured. In addition, the pH value of the normal vagina is less than 4.5, and a buffer solution is prepared, so that the gel is not influenced by the acidic environment of the vagina when being administered, the relatively stable pH value can be kept for a long time, and the effect of the gel effect is improved;

3 parts of chitosan oligosaccharide nano silver. The nano silver has strong and durable antibacterial property, and is easy to agglomerate and settle to influence the antibacterial stability due to small particle size and large specific surface area. The chitosan oligosaccharide is oligosaccharide with the polymerization degree of 2-20, which is obtained by performing biological enzymolysis or chemical degradation on chitosan, has biological activities of resisting oxidation, protecting cells, enhancing immunity and the like, and has good water solubility and easy preparation and absorption by a human body. Chitosan oligosaccharide also has certain bacteriostatic and anti-inflammatory effects, but is more limited. The chitosan oligosaccharide lipoic acid polymer with good stability is obtained by grafting lipoic acid with chitosan oligosaccharide, then covalent chelation is carried out on the surface of nano-silver by disulfide bonds in the chitosan oligosaccharide lipoic acid polymer, and surface modification is carried out on nano-silver particles, so that the chitosan oligosaccharide nano-silver with stable performance is obtained. The chitosan oligosaccharide nano silver has the advantages that the antibacterial property of the nano silver and the antibacterial property of the chitosan oligosaccharide are cooperated, so that the antibacterial efficiency is improved, and meanwhile, the biological activity of the chitosan oligosaccharide is introduced;

5 parts of glycerol, wherein the glycerol has no toxicity to human bodies, can improve the penetration capacity of the medicine to the skin, improve the medicine effect, promote the dissolution of vitamins and the like, has good lubricating capacity, and can discharge the repairing gel from the human body and promote the discharge of damaged tissues from the human body if the human body has rejection;

0.2 part of lipoxin, wherein in the existing research, the lipoxin has the function of treating inflammatory or autoimmune diseases, and can reduce the inflammation probability in repairing the damage of uterine membranes;

0.2 part of vitamin C, wherein the vitamin C is necessary for antibody and collagen formation, tissue repair (including certain redox actions), immune function maintenance, vessel integrity maintenance, non-heme iron absorption promotion and the like, and can improve the wound repair efficiency;

vitamin B₂0.2 portion of vitamin B2, the main physiological function of which is to promote metabolism as a coenzyme and has the function of diminishing inflammation.

The preparation direction of the repairing gel is as follows:

the buffer was prepared as follows: adding deionized water into a preparation container, then adding phosphoric acid and disodium hydrogen phosphate, detecting the pH of the solution by using test paper after the solution is recovered to normal temperature, then adding phosphoric acid and disodium hydrogen phosphate, and filtering after meeting the required pH, wherein the obtained filtrate is a buffer solution for later use;

the chitosan oligosaccharide nano silver is prepared as follows: 1) crosslinking chitosan oligosaccharide and lipoic acid by using EDC [1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride ] and NHS (N-hydroxysuccinimide) to obtain a chitosan oligosaccharide lipoic acid polymer, firstly preparing a 5% chitosan oligosaccharide aqueous solution, then adding 1% EDC and 0.5% NHS, dissolving 1% lipoic acid in 15 times of absolute ethyl alcohol by volume ratio until the mixture is completely dissolved, then adding the solution, stirring in a water bath at 40 ℃ for 24 hours, placing the mixture in a molecular weight cut-off 2000Da dialysis bag after stirring, dialyzing with deionized water for 3 days, changing water once every 12 hours, adding absolute ethyl alcohol into a dialysate retentate, carrying out rotary evaporation, and then freezing the dried chitosan oligosaccharide lipoic acid polymer; 2) preparing a nano silver solution, namely mixing 10 parts of 1mmol/L silver nitrate solution and 2 parts of 401mmol/L sodium citrate solution, slowly dripping the mixture into 30 parts of 10mmol/L sodium borohydride solution, quickly stirring for 30min, and aging for 2h to obtain the nano silver solution; 3) preparing the freeze-dried chitosan oligosaccharide lipoic acid polymer into 2mg/mL aqueous solution, slowly dripping 20 parts of chitosan oligosaccharide lipoic acid polymer solution into the nano-silver solution prepared in the last step, quickly stirring, putting into a dialysis bag with the molecular weight cutoff of 10000Da for dialysis, and finally freeze-drying to obtain the chitosan oligosaccharide modified nano-silver.

Sequentially adding glycerol, vitamin C and vitamin B into buffer solution at room temperature and normal pressure₂And lipoxin is uniformly stirred, and then chitosan oligosaccharide nano silver is added and fully stirred to obtain the repairing gel.

The buffer solution is prepared according to the content of the phosphoric acid and the disodium hydrogen phosphate, so that the pH value of the buffer solution is 7-8.

Example three:

the embodiment of the invention provides a uterine endometrium repair gel which comprises the following components in parts by weight: 25 parts of buffer solution consisting of phosphoric acid and disodium hydrogen phosphate, wherein the buffer solution is matched with the contents of the phosphoric acid and the disodium hydrogen phosphate, so that the pH range of the buffer solution is 7-8, the pH value of cervical secretion in the middle of a physiological cycle is 7-8.5, and the consistency of the medicine and a body fluid environment is ensured. In addition, the pH value of the normal vagina is less than 4.5, and a buffer solution is prepared, so that the gel is not influenced by the acidic environment of the vagina when being administered, the relatively stable pH value can be kept for a long time, and the effect of the gel effect is improved;

5 parts of chitosan oligosaccharide nano silver, wherein the nano silver has strong and durable antibacterial property, and is easy to agglomerate and settle to influence the antibacterial stability due to small particle size and large specific surface area. The chitosan oligosaccharide is oligosaccharide with the polymerization degree of 2-20, which is obtained by performing biological enzymolysis or chemical degradation on chitosan, has biological activities of resisting oxidation, protecting cells, enhancing immunity and the like, and has good water solubility and easy preparation and absorption by a human body. Chitosan oligosaccharide also has certain bacteriostatic and anti-inflammatory effects, but is more limited. The chitosan oligosaccharide lipoic acid polymer with good stability is obtained by grafting lipoic acid with chitosan oligosaccharide, then covalent chelation is carried out on the surface of nano-silver by disulfide bonds in the chitosan oligosaccharide lipoic acid polymer, and surface modification is carried out on nano-silver particles, so that the chitosan oligosaccharide nano-silver with stable performance is obtained. The chitosan oligosaccharide nano silver has the advantages that the antibacterial property of the nano silver and the antibacterial property of the chitosan oligosaccharide are cooperated, so that the antibacterial efficiency is improved, and meanwhile, the biological activity of the chitosan oligosaccharide is introduced;

5 parts of glycerol, wherein the glycerol has no toxicity to human bodies, can improve the penetration capacity of the medicine to the skin, improve the medicine effect, promote the dissolution of vitamins and the like, has good lubricating capacity, and can discharge the repairing gel from the human body and promote the discharge of damaged tissues from the human body if the human body has rejection;

0.2 part of lipoxin, wherein in the existing research, the lipoxin has the function of treating inflammatory or autoimmune diseases, and can reduce the inflammation probability in repairing the damage of uterine membranes;

0.2 part of vitamin C, wherein the vitamin C is necessary for antibody and collagen formation, tissue repair (including certain redox actions), immune function maintenance, vessel integrity maintenance, non-heme iron absorption promotion and the like, and can improve the wound repair efficiency;

vitamin B₂0.3 portion of vitamin B2, the main physiological function of which is to promote metabolism as a coenzyme and has the function of diminishing inflammation.

The preparation direction of the repairing gel is as follows:

the buffer was prepared as follows: adding deionized water into a preparation container, then adding phosphoric acid and disodium hydrogen phosphate, detecting the pH of the solution by using test paper after the solution is recovered to normal temperature, then adding phosphoric acid and disodium hydrogen phosphate, and filtering after meeting the required pH, wherein the obtained filtrate is a buffer solution for later use;

the chitosan oligosaccharide nano silver is prepared as follows: 1) crosslinking chitosan oligosaccharide and lipoic acid by using EDC [1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride ] and NHS (N-hydroxysuccinimide) to obtain a chitosan oligosaccharide lipoic acid polymer, firstly preparing a 5% chitosan oligosaccharide aqueous solution, then adding 1% EDC and 0.5% NHS, dissolving 1% lipoic acid in 15 times of absolute ethyl alcohol by volume ratio until the mixture is completely dissolved, then adding the solution, stirring in a water bath at 40 ℃ for 24 hours, placing the mixture in a molecular weight cut-off 2000Da dialysis bag after stirring, dialyzing with deionized water for 3 days, changing water once every 12 hours, adding absolute ethyl alcohol into a dialysate retentate, carrying out rotary evaporation, and then freezing the dried chitosan oligosaccharide lipoic acid polymer; 2) preparing a nano silver solution, namely mixing 10 parts of 1mmol/L silver nitrate solution and 2 parts of 401mmol/L sodium citrate solution, slowly dripping the mixture into 30 parts of 10mmol/L sodium borohydride solution, quickly stirring for 30min, and aging for 2h to obtain the nano silver solution; 3) preparing the freeze-dried chitosan oligosaccharide lipoic acid polymer into 2mg/mL aqueous solution, slowly dripping 20 parts of chitosan oligosaccharide lipoic acid polymer solution into the nano-silver solution prepared in the last step, quickly stirring, putting into a dialysis bag with the molecular weight cutoff of 10000Da for dialysis, and finally freeze-drying to obtain the chitosan oligosaccharide modified nano-silver.

Sequentially adding glycerol, vitamin C and vitamin B into buffer solution at room temperature and normal pressure₂And lipoxin is uniformly stirred, and then chitosan oligosaccharide nano silver is added and fully stirred to obtain the repairing gel.

The buffer solution is prepared according to the content of the phosphoric acid and the disodium hydrogen phosphate, so that the pH value of the buffer solution is 7-8.

Example four:

selecting a plurality of female mice, wherein the female mice have the weight of 250 +/-20 g, culturing for 3-7 days, and adding epimedium into the feed of the mice to promote the oestrus of the mice and improve the activity of a breeding system.

Female mice with poor vitality in the breeding are removed and then grouped, and at least 5 female mice are randomly selected in each group. Mechanical endometrial lesions were developed in female mice using mechanical uterine curettage: first, 10% chloral hydrate was used for anesthesia, the lower abdomen was disinfected with 75% alcohol, the skin was cut at 1cm of the upper end of the urethra into the abdominal cavity, the uterus was exposed, and the endometrium was treated with a uterine curettage tool, simulating clinical human endometrium curettage, ensuring that the treatment pattern of each female mouse was consistent, and then the wound was sutured.

Then, 1.5ml of the gel produced in example one, 1.5ml of the gel produced in example two, 1.5ml of the gel produced in example three, 2.0ml of the gel produced in example three, 2.5ml of the gel produced in example three, and 1.5ml of physiological saline were injected into the uterus of the female rat, and then the female rat was normally raised for 2 to 3 days, followed by anatomical observation of the uterus of the female rat, and judgment was made as follows:

group of	Mode of production	Injection volume (ml)	Uterine membrane	Recovery situation
					Experimental group 1	Practice ofExample one	1.5	No inflammation and edema	Good effect
Experimental group 2	Example two	1.5	No inflammation and edema	Good effect
					Experimental group 3	EXAMPLE III	1.5	No inflammation and edema	Good effect
Experimental group 4	EXAMPLE III	2.0	No inflammation and edema	Superior food
					Experimental group 5	EXAMPLE III	2.5	No inflammation and edema	Good effect
Control group	Physiological saline	1.5	Inflammation and edema	In general

Note: the recovery condition is comprehensively judged according to the vitality of the female mouse during the feeding period, such as food intake, activity and the estimated recovery time of the uterine membrane.

The repair gel prepared by the invention has the antibacterial and anti-inflammatory effects, the drug dosage is reasonably given, the repair speed of the uterine membrane can be increased, and the best effect cannot be achieved by too much or too little dosage.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.