阅读说明：本技术 强效抗衰老的枇杷花提取物在制备抗衰老化妆品中的应用 (Application of loquat flower extract with strong anti-aging effect in preparation of anti-aging cosmetics ) 是由 龚盛昭 郑丹阳 黎晓燕 林彤彤 陈杰生 于 2021-08-06 设计创作，主要内容包括：本发明公开一种强效抗衰老的枇杷花提取物在制备抗衰老化妆品中的应用,属于化妆品领域。该枇杷花提取物的制备方法为：将枇杷花粉碎,加入提取溶剂(乙醇与水的混合溶液),用常温高压均质法提取,得到提取液,经离心分离后,离心液用大孔树脂吸附,用乙醇水溶液洗脱,洗脱液减压浓缩后加入1,3-丁二醇,制得枇杷花提取物。利用该方法获得的枇杷花提取物天然安全,能强效促进人胚肺成纤维细胞增殖和生长,强效清除自由基,抑制酪氨酸酶活性,减少黑色素生成,加快皮肤表层细胞更新,延缓肌肤衰老,添加于抗衰老化妆品中,制出性质稳定、效果良好的抗衰老化妆品,抗衰老功效显著。(The invention discloses application of a loquat flower extract with strong anti-aging effect in preparing anti-aging cosmetics, and belongs to the field of cosmetics. The preparation method of the loquat flower extract comprises the following steps: pulverizing flos Eriobotryae, adding extraction solvent (mixed solution of ethanol and water), homogenizing at normal temperature under high pressure to obtain extractive solution, centrifuging, adsorbing centrifugate with macroporous resin, eluting with ethanol water solution, concentrating eluate under reduced pressure, and adding 1, 3-butanediol to obtain flos Eriobotryae extract. The loquat flower extract obtained by the method is natural and safe, can strongly promote the proliferation and growth of human embryonic lung fibroblasts, strongly remove free radicals, inhibit tyrosinase activity, reduce melanin generation, accelerate the renewal of skin surface cells and delay skin aging, and is added into an anti-aging cosmetic to prepare the anti-aging cosmetic with stable property and good effect, and the anti-aging effect is remarkable.)

1. Application of loquat flower extract with strong anti-aging effect in preparing anti-aging cosmetics.

2. Use according to claim 1, characterized in that:

the addition amount of the loquat flower extract with strong anti-aging effect in the anti-aging cosmetic is 0.1-25% by mass.

3. Use according to claim 2, characterized in that:

the addition amount of the loquat flower extract with strong anti-aging effect in the anti-aging cosmetic is 1-10% by mass.

4. Use according to claim 3, characterized in that:

the addition amount of the loquat flower extract with strong anti-aging effect in the anti-aging cosmetic is 4.5-6% by mass.

5. Use according to any one of claims 1 to 4, characterized in that:

the anti-aging cosmetic comprises the following components: aqueous preparations, emulsions, creams, gels, powders, powder-forming preparations, facial masks, sprays, and essences.

6. Use according to any one of claims 1 to 4, characterized in that:

the anti-aging cosmetic is anti-aging nutrition cream and comprises the following components in parts by mass: 2.5% of cetearyl olive oleate, 0.8% of sorbitan olive oleate, 0.6% of cetearyl glucoside, 4.0% of caprylic capric glyceride, 4.0% of isopropyl palmitate, 2.0% of shea butter, 3.0% of hydrogenated polydecene, 4.0% of cyclopolydimethylsiloxane, 0.15% of methylparaben, 0.10% of propylparaben, 6.0% of loquat flower extract, 0.1% of allantoin, 0.50% of dextran, 0.15% of xanthan gum, 0.02% of disodium EDTA, 10.0% of glycerol, 0.05% of sodium hyaluronate, 0.20% of phenoxyethanol, 0.15% of essence, and water up to 100%;

the anti-aging cosmetic is anti-aging emulsion and comprises the following components in percentage by mass: 0.50% of cetostearyl alcohol, 0.30% of shea butter, 0.15% of dioctyl carbonate, 0.10% of cyclomethicone, 3.00% of glyceryl polyacrylate, 3.00% of C14-22 alcohol, 1.00% of C12-20 alkyl glucoside, 1.00% of cetearyl glucoside, 1.50% of di-C12-13 alcohol malate, 0.10% of EDTA-2Na, 1.00% of D-pantothenic acid, 1.00% of glyceryl polyacrylate, 3.00% of butanediol, 0.50% of carbomer, 1.00% of aloe vera leaf extract, 4.50% of loquat flower extract and deionized water added to 100%;

the anti-aging cosmetic is anti-aging mask liquid and comprises the following components in parts by mass:

0.03% of EDTA-2Na, 0.20% of hydroxyethyl cellulose, 2.80% of glycerol polyacrylate, 0.12% of xanthan gum, 7.00% of 1, 3-butanediol, 0.08% of sodium hyaluronate, 1.00% of aloe barbadensis leaf extract, 5.00% of loquat flower extract, 0.30% of menthone, 0.50% of 1, 2-hexanediol, 0.20% of phenoxyethanol and deionized water to 100%;

the anti-aging cosmetic is anti-aging gel, and comprises the following components in parts by mass:

0.02 percent of EDTA-2Na, 0.10 percent of allantoin, 0.50 percent of DL-panthenol, 1.00 percent of natural betaine, 5.00 percent of PEG-26 glyceryl ether, 2.00 percent of glyceryl polyacrylate, 6.00 percent of glycerin, 0.10 percent of sodium hyaluronate, 0.30 percent of carbomer U21, 0.25 percent of acrylamide dimethyl taurate/VP copolymer, 3.00 percent of 1, 3-butanediol, 0.30 percent of carnation ketone, 0.50 percent of 1, 2-hexanediol, 0.20 percent of phenoxyethanol,COOLING COMPLEX 0.50%, beta-glucan 1.00%, loquat flower extract 4.50%, maltooligosaccharide glucoside 1.00%, hydrogenated starch hydrolysate 1.00%, ethanol 5.00%, hydrogenated castor oil CO40 0.30%, essence 0.03%, aminomethyl propanol 0.18%, deionized water to 100%.

7. Use according to any one of claims 1 to 4, characterized in that:

the preparation method of the loquat flower extract with strong anti-aging effect comprises the following steps:

firstly, pulverizing loquat flowers, sieving the pulverized loquat flowers by using a sieve with 20-120 meshes, and mixing the sieved loquat flowers and the sieve to obtain loquat flower powder;

secondly, according to the mass ratio of liquid to material of 2-20: 1, adding an extraction solvent, soaking for 0.5-6 h, and then extracting at normal temperature by using a high-pressure homogenizing extractor under the extraction pressure of 2-20 MPa to obtain an extracting solution; the extraction solvent is a mixed solution of ethanol and water;

thirdly, after the extracting solution is subjected to centrifugal separation, adsorbing the centrifugate by using macroporous resin, and eluting by using ethanol water solution with the volume fraction of 40-95%;

fourthly, concentrating the eluent under reduced pressure until the mass ratio of the concentrated solution to the loquat pollen is 1: 1, adding 1, 3-butanediol with the same mass as the concentrated solution to obtain the loquat flower extract.

8. Use according to claim 7, characterized in that:

screening by using a 40-100-mesh sieve;

the mass ratio of the liquid to the material in the second step is 5-15: 1;

the extraction solvent is a mixed solution of ethanol and water, and the volume fraction of the mixed solution is 20-60% ethanol water solution;

the extraction pressure in the second step is 4-10 MPa;

and step three, eluting with ethanol water solution with the volume fraction of 50-95%.

9. Use according to claim 8, characterized in that:

screening by using a 40-80-mesh sieve;

the mass ratio of the liquid to the material in the second step is 10-15: 1;

the extraction solvent is a mixed solution of ethanol and water, and the volume fraction of the mixed solution is 40-60% ethanol water solution;

the extraction pressure in the second step is 4-6 MPa;

and step three, eluting with ethanol water solution with the volume fraction of 70%.

10. Use according to claim 7, characterized in that:

soaking for 2-3 hours;

the radius of the centrifugation in the third step is 8-12 cm;

centrifuging at the normal temperature for 15-60 min at 500-4000 rpm;

in the third step, the centrifugate is absorbed by AB-8 macroporous absorbent resin;

the normal temperature is 15-40 ℃.

Technical Field

The invention relates to a traditional Chinese medicine extract, in particular to application of a loquat flower extract with strong anti-aging effect in preparing anti-aging cosmetics, and belongs to the field of cosmetics.

Background

Skin aging is a normal phenomenon, which is always present from birth, and as a result, in addition to the physiological function of skin, it is most important to change the appearance of human beings. People often judge the age, identity and status of a person by some extrinsic features of the skin and hair. Aging causes beauty and color reduction, and also has negative effects on human psychology, so that people all want to keep youthful appearance and youthful and eternal appearance.

Preventing and delaying skin aging is an important subject of beauty skin science. The development of anti-aging active substances with obvious effect, safety, stability and good absorption becomes a hot spot of the current beauty industry, and the anti-aging active substances of natural traditional Chinese medicines with plant sources are the main research directions.

Disclosure of Invention

In order to overcome the defects of the prior art, the invention mainly aims to provide the application of the loquat flower extract with strong anti-aging effect in preparing anti-aging cosmetics. The flos Eriobotryae extract is natural and safe, and has effects of strongly activating skin cell proliferation and growth, inhibiting tyrosinase, and scavenging free radicals in vivo; can be used in anti-aging cosmetics to prepare anti-aging cosmetics with stable properties and good effect.

The purpose of the invention is realized by the following technical scheme:

the invention provides application of a loquat flower extract with strong anti-aging effect in preparing anti-aging cosmetics.

The addition amount of the loquat flower extract with strong anti-aging effect in the anti-aging cosmetic is 0.1-25% by mass, preferably 1-10% by mass, and further 4.5-6% by mass.

The anti-aging cosmetic comprises the following components: aqueous preparations, emulsions, creams, gels, powders, powder-forming preparations, facial masks, sprays, and essences.

Specifically, the invention provides anti-aging nutrient cream which comprises the following components in parts by mass:

name of raw materials	Mass fraction (%)
		Cetearyl olive oleate	2.5
Sorbitan Olive oleate	0.8
		Cetearyl glucoside	0.6
Caprylic capric acid glyceride	4.0
		Palmitic acid isopropyl ester	4.0
Shea butter	2.0
		Hydrogenated polydecene	3.0
Cyclopolydimethylsiloxane	4.0
		Hydroxy phenyl methyl ester	0.15
Propyl hydroxybenzoate	0.10
		Water (W)	To 100 of
Loquat flower extract	6.0
		Allantoin	0.1
Glucan	0.50
		Chinese gum	0.15
EDTA disodium salt	0.02
		Glycerol	10.0
Hyaluronic acid sodium salt	0.05
		Phenoxyethanol	0.20
Essence	0.15

Specifically, the invention provides an anti-aging emulsion which comprises the following components in parts by mass:

specifically, the invention provides an anti-aging mask liquid which comprises the following components in parts by mass:

name of raw materials	Mass fraction (%)
		Deionized water	To 100 of
EDTA-2Na	0.03
		Hydroxyethyl cellulose	0.20
Glycerol polyacrylates	2.80
		Xanthan gum	0.12
1, 3-butanediol	7.00
		Hyaluronic acid sodium salt	0.08
Aloe barbadensis leaf extract	1.00
		Loquat flower extract	5.00
Xinxian Ketone	0.30
		1, 2-hexanediol	0.50
Phenoxyethanol	0.20

Specifically, the invention provides anti-aging gel which comprises the following components in parts by mass:

the preparation method of the loquat flower extract with strong anti-aging effect comprises the following steps:

firstly, pulverizing loquat flowers, sieving the pulverized loquat flowers by using a sieve with 20-120 meshes, and mixing the sieved loquat flowers and the sieve to obtain loquat flower powder;

secondly, according to the mass ratio of liquid to material of 2-20: 1, adding an extraction solvent, soaking for 0.5-6 h, and then extracting at normal temperature by using a high-pressure homogenizing extractor under the extraction pressure of 2-20 MPa to obtain an extracting solution; the extraction solvent is a mixed solution of ethanol and water;

thirdly, after the extracting solution is subjected to centrifugal separation, adsorbing the centrifugate by using macroporous resin, and eluting by using ethanol water solution with the volume fraction of 40-95%;

fourthly, concentrating the eluent under reduced pressure until the mass ratio of the concentrated solution to the loquat pollen is 1: 1, adding 1, 3-butanediol with the same mass as the concentrated solution to obtain the loquat flower extract.

Preferably, in the step I, screening by using a 40-100-mesh sieve; and further sieving the mixture by using a 40-80-mesh sieve.

Preferably, the mass ratio of the liquid to the material in the step II is 5-15: 1; further 10-15: 1.

preferably, the soaking time in the step II is 2-3 h.

Preferably, the extraction solvent in the second step is a mixed solution of ethanol and water, and the volume fraction of the mixed solution is 20-60% ethanol water solution; furthermore, the volume fraction is 40-60% of ethanol water solution.

Preferably, the extraction pressure in the step II is 4-10 MPa; further 4 to 6 MPa.

Preferably, the radius of the centrifugation in the third step is 8-12 cm.

Preferably, the centrifugation conditions in the third step are 500-4000 rpm and normal temperature centrifugation for 15-60 min; further centrifuging at 3000rpm and normal temperature for 20-40 min.

Preferably, in the third step, the centrifugate is absorbed by AB-8 macroporous absorbent resin.

Preferably, in the step III, ethanol water solution with volume fraction of 50-95% is used for elution; further, the eluate was eluted with 70% by volume of an aqueous ethanol solution.

The normal temperature in the invention is 15-40 ℃.

Compared with the prior art, the invention has the following advantages and effects:

(1) loquat flower is flower of Eriobotrya japonica (Thunb.) lindl of Rosaceae, and has effects of dispelling pathogenic wind, relieving cough, and dredging nasal orifice. It is commonly used to treat wind syndrome of the head, nasal obstruction, nasal discharge, chronic cough due to asthenia, and bloody sputum. The invention innovatively discovers that the extract of the loquat flower also has the effects of strongly activating the growth of skin cells, inhibiting tyrosinase, removing free radicals in vivo and the like through research, and is a very good anti-aging active substance.

In order to obtain the best extraction effect, the invention firstly carries out scientific experiments on the extraction solvent, uses water, ethanol, 1, 3-butanediol, PEG400, petroleum ether and the like as the extraction solvent respectively, and finds that the ethanol aqueous solution has the best effect. The heating extraction method, the microwave extraction method, the ultrasonic extraction method, the supercritical extraction method and the high-pressure homogenization extraction method are respectively adopted, and the result shows that the high-pressure homogenization extraction method has the best effect. A great deal of scientific research is carried out on the extraction conditions.

In order to obtain the best separation and purification effect, membrane separation and macroporous resin separation are respectively adopted, and as a result, the activity of the extract is obviously improved after the membrane separation and the macroporous resin separation are found, and the activity of the extract obtained by the macroporous resin separation is better, and the conductivity of the extract is less than 300 mu S/cm and is much lower than that of the extract obtained by a membrane separation method (about 1200 mu S/cm). The scientific experiments of the separation of the extracts are respectively carried out by using the types of macroporous resins of AB-8, X-5, D101, LSA-20 and the like, and the results show that the effect is the best after the treatment by the AB-8 macroporous absorption resin. The eluents were examined for 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95% and 100% ethanol by volume fraction, respectively, and it was found that 70% ethanol elution gave the best results of the extract.

The extraction and separation processes are carried out at normal temperature to ensure that the active ingredients are not deactivated by heat treatment.

(2) The loquat flower extract obtained by the method can strongly promote the proliferation and growth of human embryonic lung fibroblasts, strongly remove free radicals, inhibit the activity of tyrosinase, reduce the generation of melanin, accelerate the renewal of skin surface cells and delay skin aging, and has obvious anti-aging effect when being added into cosmetics.

Drawings

FIG. 1 is the effect of loquat flower extract on cell growth curves.

Detailed Description

The present invention will be described in further detail with reference to examples and drawings, but the present invention is not limited thereto.

The test methods in the following examples, in which specific experimental conditions are not specified, are generally performed according to conventional experimental conditions or according to the experimental conditions recommended by the manufacturer. The materials, reagents and the like used are, unless otherwise specified, reagents and materials obtained from commercial sources.

EXAMPLE 1 preparation of loquat flower extract (I)

1kg of dried loquat flower is taken, the loquat flower is crushed, screened by a 60-mesh sieve and mixed to obtain loquat flower powder;

secondly, according to the mass ratio of liquid to material of 12: 1 adding an extraction solvent, soaking for 2.5h, and extracting at normal temperature by using a high-pressure homogenizing extractor under the extraction pressure of 5MPa to obtain an extracting solution; the extraction solvent is ethanol water solution with the volume fraction of 50 percent;

③ centrifuging the extract (centrifugation radius is 10cm, centrifugation condition is 3000rpm, normal temperature centrifugation is 30min), adsorbing the centrifugate by AB-8 macroporous adsorbent resin (purchased from Tianjin Praded Biotech limited) and eluting by ethanol water solution with volume fraction of 70%;

fourthly, concentrating the eluent under reduced pressure until the mass ratio of the concentrated solution to the loquat pollen is 1: 1, adding 1, 3-butanediol with the same mass as the concentrated solution to obtain the loquat flower extract (I).

Example 2 preparation of loquat flower extract (II)

1kg of dried loquat flower is taken, the loquat flower is crushed, screened by a 80-mesh sieve and mixed to obtain loquat flower powder;

secondly, according to the mass ratio of liquid to material of 15: 1, adding an extraction solvent, soaking for 2 hours, and then extracting at normal temperature by using a high-pressure homogenizing extractor under the extraction pressure of 4MPa to obtain an extracting solution; the extraction solvent is ethanol water solution with the volume fraction of 40 percent;

③ centrifuging the extract (centrifugation radius is 12cm, centrifugation condition is 3000rpm, normal temperature centrifugation is 20min), adsorbing the centrifugate by AB-8 macroporous adsorption resin, and eluting by ethanol water solution with volume fraction of 70%;

fourthly, concentrating the eluent under reduced pressure until the mass ratio of the concentrated solution to the loquat pollen is 1: 1, adding 1, 3-butanediol with the same mass as the concentrated solution to obtain the loquat flower extract (II).

Example 3 preparation of loquat flower extract (III)

1kg of dried loquat flower is taken, the loquat flower is crushed, screened by a 40-mesh sieve and mixed to obtain loquat flower powder;

secondly, according to the mass ratio of liquid to material of 10: 1 adding an extraction solvent, soaking for 3 hours, and then extracting at normal temperature by using a high-pressure homogenizing extractor under the extraction pressure of 6MPa to obtain an extracting solution; the extraction solvent is ethanol water solution with the volume fraction of 60%;

③ centrifuging the extract (centrifugation radius is 8cm, centrifugation condition is 3000rpm, normal temperature centrifugation is 40min), adsorbing the centrifugate by AB-8 macroporous adsorption resin, and eluting by ethanol water solution with volume fraction of 70%;

fourthly, concentrating the eluent under reduced pressure until the mass ratio of the concentrated solution to the loquat pollen is 1: 1, adding 1, 3-butanediol with the same mass as the concentrated solution to obtain the loquat flower extract (III).

Test example 1 Effect of loquat flower extract on cell proliferation

The MTT experiment detection principle is as follows: succinate dehydrogenase in mitochondria of living cells can reduce exogenous MTT (thiazole blue) into water-insoluble blue-violet crystalline formazan and deposit in the cells, but dead cells do not have the function; then, the formazan in the cells is dissolved by DMSO, and the measurement is carried out at 570nm by a microplate reader; since the production amount of formazan is generally proportional to the number of living cells, the number of living cells can be reflected by the OD number measured by the microplate reader.

Suspending MRC5 cells (selected from MRC5 cells, normal human embryonic lung fibroblasts, conventional commercial product) at 5 × 10³Inoculating the loquat flowers into a 96-well cell culture plate per well, adding 100 mu L of fresh MEM culture medium containing the loquat flower extract of the embodiment 1-3 with the mass fraction of 0.1% after the cells adhere to the wall, and arranging 3 multiple wells for each component; constant temperature of 37 ℃ and 5% CO₂After culturing for 48h in the incubator, adding 10 mu L of MTT solution containing 5 mg/mL; terminating the culture after 4h, sucking out the culture medium in the holes, and adding 150 mu L of DMSO into each hole to fully dissolve the formazan ; the absorbance of each well was then measured using a microplate reader. At the same time, zero-setting wells (culture medium, MTT, DMSO) and control wells (cells, containing the same volume as the extract) are providedDMSO medium, MTT, DMSO). The results are shown in Table 1.

TABLE 1

Experimental project	Example 1	Example 2	Example 3	Blank control
					MRC5 cell viability	131％	132％	130％	100％

The result shows that the loquat flower extract of the invention obviously promotes the proliferation of MRC5 cells and has obvious anti-aging effect.

Test example 2 Effect of loquat flower extract on cell growth Curve

To further verify the long-term effect of the loquat flower extract on cell viability, the long-term growth relationship of MRC5 cells was determined in terms of days and PD (population doubling) using the loquat flower extract of example 1, which accurately describes the growth of cells throughout the culture. The MRC5 cells were selected as normal human embryonic lung fibroblasts that had only limited ability to divide in vitro and then entered the senescence stage. The PD number is calculated as Y-Y0 + log₂Nt/N0, wherein N0 is primordial cellThe number of cells was determined as Nt and the number of PD in the case of primary cells as Y0.

The long-term growth curve of MRC5 cells was plotted on the abscissa of the number of days in culture and the ordinate of the PD number, and the results are shown in FIG. 1.

As can be seen from fig. 1, the PD number of the loquat flower extract-treated cells entering the plateau phase was significantly higher than that of the control group, which reflects that the loquat flower extract plays a role in significantly delaying the entry of MRC5 cells into the senescence process.

Test example 3 Effect of loquat flower extract on tyrosinase and free radical

1. Preparation of the test specimens

The loquat flower extract was prepared as a sample solution with a concentration of 1%, and the following experiment was performed.

2. Evaluation of tyrosinase Activity inhibitory ability

Adding samples according to the table 2, comparing without adding tyrosinase as a control, and placing the solution in a water bath at 37 ℃ for constant temperature for 10 min; tyrosinase (110U/mL) was added, and the solution was incubated in a water bath at 37 ℃ for 10min, and then the absorbance value was measured at 475 nm.

TABLE 2

The relative inhibition rate (I, inhibition rate for short, the same below) of the sample on tyrosinase was calculated according to the formula (1).

In the formula: a. the₁、A₂、A₃、A₄The absorbance of the solutions of groups 1 to 4, respectively.

3. Evaluation of superoxide anion radical scavenging ability

4.5mL of Tris-HCl buffer (0.05 mol/L, pH 8.2) was preheated in a water bath at 25 ℃ for 20 min. Then adding 1mL of sample and 0.4mL of 25mmol/L pyrogallol solution, mixing uniformly, reacting in a water bath at 25 ℃ for 5min, and adding 1.0mL of 8mol/L HCl to terminate the reaction. Absorbance values were measured at 299nm with Tris-HCl buffer as a reference. Blank control 1mL of sample in solvent was used instead of sample. The clearance (D) was calculated according to equation (2).

Superoxide anion radical scavenging rate (%) ([ 1- (A)₂/A₁)]×100％ (2)

In the formula A₁Absorbance values for the blank; a. the₂Is the absorbance value of the sample.

4. Evaluation of hydroxyl radical scavenging ability

Sequentially adding 2mmol/L FeSO into a 25mL colorimetric tube₄ 3mL，1mmol/L H₂O₂3mL, shaking up, then adding 3mL of salicylic acid with the concentration of 6mmol/L, shaking up, heating in a water bath at 37 ℃ for 15min, taking out, and measuring the absorbance at 510 nm; adding the samples to be tested respectively, shaking up, continuing to heat in water bath for 15min, taking out and testing the absorbance at 510 nm. The following formula is the clearance rate of hydroxyl radical (. OH) of the sample to be tested:

hydroxyl radical clearance (%) ═ a₁－A₂－(A₁－A₃)]/A₁×100％ (3)

In the formula A₁The absorbance value of the reaction system before adding the medicine is obtained; a. the₂The absorbance value of the system after eliminating OH for the medicine; a. the₃Absorbance value of the system after clearing-OH for blank control.

5. Results of the experiment

The anti-aging function evaluation test is shown in table 3.

TABLE 3

Experimental project	Example 1	Example 2	Example 3
				Inhibition of tyrosinase activity/%)	82.3	83.1	81.5
For superoxide anion removal%	93.1	91.6	92.3
				Hydroxy radical scavenging rate/%)	92.9	91.9	91.2

Experiments show that the loquat flower extract can effectively remove free radicals, has good tyrosinase activity inhibition effect and has obvious anti-aging function.

Application example 1 anti-aging nutrient cream

Table 4 shows the formula of the anti-aging cream containing the loquat flower extract of the present invention.

TABLE 4

The preparation method comprises the following steps: dissolving phase A at 80 deg.C, mixing phase B at 80 deg.C, adding phase B before emulsifying cyclomethicone, adding phase B during emulsifying, homogenizing for 5min, cooling to 50 deg.C, adding phase C, homogenizing for 2 min, aging for 24 hr, and packaging.

Application example 2 anti-aging emulsion

Table 5 shows the formula of an anti-aging emulsion containing the loquat flower extract of the present invention.

TABLE 5

The preparation method comprises the following steps:

dispersing the uniform phase C in advance for later use; putting the phase A component into an oil phase pot, heating to 80 ℃, and stirring until the phase A component is completely dissolved; putting the phase B component into an emulsifying pot, heating to 80 ℃, and stirring to dissolve until the phase B component is completely transparent; keeping the temperature of an emulsifying pot at 80 ℃, controlling the stirring speed to be in a vacuum foamless state of 300r/min, and slowly pumping the phase A; homogenizing at 3000r/min, and emulsifying at 80 deg.C for 10 min; after homogenizing, putting the C phase component into an emulsifying pot, keeping the C phase component in a vacuum state, stirring uniformly under the condition of 400r/min, and cooling; adding the heat sensitive component D phase component into an emulsifying pot at 50 ℃, and stirring until the components are uniformly mixed; cooling to 38 deg.C, discharging, filling and sealing.

Application example 3 anti-aging facial mask solution

Table 6 shows the formula of an anti-aging emulsion mask solution containing the loquat flower extract of the present invention.

TABLE 6

The preparation method comprises the following steps: dispersing the phase B in advance for later use; putting the phase A component and the dispersed and uniform phase B into an emulsifying pot, heating to 80 ℃, stirring to dissolve until the components are completely transparent, and then cooling; cooling to 45 deg.C, adding the C phase active substance and D phase into emulsifying pot, stirring and dissolving to mix well; cooling to 38 deg.C, discharging, filling and sealing.

Application example 4 anti-aging gel

Table 7 shows an anti-aging gel formulation containing the loquat flower extract of the present invention.

TABLE 7

The preparation method comprises the following steps: putting the raw material of the component A into a manufacturing pot, heating to 70 ℃, starting stirring and homogenizing at 3000rpm for 10min until the raw material is uniformly dispersed. Cooling to 45 deg.C, adding the pre-mixed and dissolved component B and the pre-mixed and dissolved component C, stirring for dissolving completely, vacuum degassing, filtering and discharging.

Application comparative example 1 ordinary emulsion

The results are in accordance with Table 5 except that loquat flower extract (I) was not contained.

Effect example 1 human body trial evaluation experiment

1. Experimental methods

Human body trial evaluation was performed using the emulsions prepared in example 2 and comparative example 1, respectively. 50 volunteers of 40-50 years old are selected and all female. The application method was to wash the face twice a day (once in the morning and evening), the emulsion prepared in application example 2 was applied to the left face, and the emulsion prepared in application comparative example 1 was applied to the right face. The experimental period was two months, and the skin color and wrinkles of the left and right faces were observed after two months of use.

2. Results of the experiment

After two months of use, the skin color of the left face using the lotion prepared according to application example 2 was significantly better than the skin color of the right face using the lotion prepared according to application comparative example 1 for 43 users, as determined by VISA skin detector and comparison with the skin color before use, and the wrinkles on the left face were significantly improved and the wrinkles on the right face were not improved, and the effective rate was 86%. Wherein, comprehensive evaluation is carried out from humidity retention, glossiness, compactness and anti-wrinkle effect, wherein the highest score is 5, the lowest score is 1, and the score higher than 3.5 is improved.

The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.