阅读说明：本技术 防治黄栌枯萎病的方法 (Method for preventing and treating cotinus coggygria wilt ) 是由 王永林 李奇岩 郭瑞峰 唐晨 宋立州 田呈明 李学武 于 2021-08-26 设计创作，主要内容包括：本发明涉及林业病害防治技术领域,具体而言,涉及一种防治黄栌枯萎病的方法,包括以下步骤,对黄栌植株的根部施加第一抗黄栌枯萎病药剂；以及对黄栌植株的树干注射第二抗黄栌枯萎病药剂；所述第一抗黄栌枯萎病药剂为枯草芽孢杆菌溶液,所述第二抗黄栌枯萎病药剂为咪鲜胺溶液或多菌灵溶液。相比较单一的防治方法,本发明提供的防治黄栌枯萎病的方法,具有更佳的防治效果。(The invention relates to the technical field of forestry disease control, in particular to a method for controlling cotinus coggygria fusarium wilt, which comprises the following steps of applying a first cotinus coggygria fusarium wilt resistant agent to the root of a cotinus coggygria plant; and injecting a second smoke tree blast resistant agent into the trunk of the smoke tree plant; the first anti smoke tree blight medicament is bacillus subtilis solution, the second anti smoke tree blight medicament is prochloraz solution or carbendazim solution. Compared with a single prevention and treatment method, the method for preventing and treating the cotinus coggygria fusarium wilt has better prevention and treatment effect.)

1. A method for preventing and treating cotinus coggygria wilt is characterized by comprising the following steps:

applying a first agent against cotinus coggygria blight to the roots of the cotinus coggygria plant; and

injecting a second cotinus coggygria blast resistant agent into the trunk of the cotinus coggygria plant;

the first anti smoke tree blight medicament is bacillus subtilis solution, the second anti smoke tree blight medicament is prochloraz solution or carbendazim solution.

2. The method for preventing and treating cotinus coggygria wilt disease of claim 1, wherein the concentration of bacillus subtilis in said bacillus subtilis solution is between 0.17 and 0.25 billion spores/mL.

3. The method for preventing and treating cotinus coggygria wilt disease of claim 1, wherein the concentration of the prochloraz active ingredient in the prochloraz solution is 0.450mg/mL to 0.563 mg/mL.

4. The method of claim 1, wherein said concentration of said polymyxa mobility ingredient in said carbendazim solution is from 0.50mg/mL to 0.625 mg/mL.

5. The method of preventing cotinus coggygria wilt disease of claim 1, wherein said first anti-cotinus coggygria wilt disease agent is a bacillus subtilis solution and said second anti-cotinus coggygria wilt disease agent is a carbendazim solution.

6. The method of claim 1, wherein said step of applying said first agent to said root of said smoke tree is applying said first agent 2 times at a rate of from about 400mL/cm (trunk diameter) to about 600mL/cm (trunk diameter).

7. The method of claim 6, wherein the time interval between two consecutive administrations is between 10 days and 15 days.

8. The method for preventing and treating cotinus coggygria wilt disease of any one of claims 1-7, wherein said step of injecting said second agent against cotinus coggygria wilt disease into said tree is to inject said second agent against cotinus coggygria wilt disease into said tree for 2-5 times, wherein said second agent is administered in an amount of 200mL/cm (trunk ground diameter) to 250mL/cm (trunk ground diameter).

9. The method of claim 8, wherein the time interval between two consecutive administrations is between 10 days and 15 days.

10. The method for preventing and treating cotinus coggygria wilt disease of any one of claims 1-7 and 9, wherein the injection of the second agent against cotinus coggygria wilt disease into the trunk of the cotinus coggygria plant is performed at a location of 0-80 cm from the branch-off end of the diseased branch.

Technical Field

The invention relates to the technical field of forestry disease control, in particular to a method for controlling cotinus coggygria wilt.

Background

Cotinus coggygria Scop, plants of Cotinus coggygria of Anacardiaceae, deciduous shrubs or small trees are important tree species for landscaping, ornamental tree species, barren mountain forestation and farmland protection forests in China, and have ornamental, medicinal, ecological and economic values. The cotinus coggygria is a main tree species of Beijing red-leaf landscape, and leaves of the cotinus coggygria can turn red in autumn to attract a large number of citizen red-leaf landscape.

At present, the common coggygria fusarium wilt seriously affects the health of the common coggygria and red-leaf landscape construction, and the disease is seriously generated in Beijing Xiangshan, Ba Da Ling and the like. In Xiangshan, the incidence rate is as high as 100%. The cotinus coggygria wilt is a systemic infectious disease, which causes root system decay, leaf etiolation necrosis, branch withering and even whole plant death, and the cotinus coggygria wilt becomes a main biological disaster affecting the health of cotinus coggygria and the ecological construction of red leaf landscape, and the control of epidemic and disaster formation of the disease becomes one of the most main tasks for ensuring the health of cotinus coggygria.

The pathogenic bacteria of the Cotinus coggygria wilt disease is Verticillium dahliae, and the bacteria colonize vascular bundles, so the spray control effect is extremely poor; aiming at the problem that the operability of treatment methods such as soil disinfection, fumigation, insolation and the like in the forest land is extremely low, the existing control method mainly adopts single root irrigation, and the control effect is poor.

Disclosure of Invention

Based on this, the present invention provides a more effective method for the control of cotinus coggygria blight.

On one hand, the invention provides a method for preventing and treating cotinus coggygria wilt, which comprises the following steps:

applying a first agent against cotinus coggygria blight to the roots of the cotinus coggygria plant; and

injecting a second cotinus coggygria blast resistant agent into the trunk of the cotinus coggygria plant;

the first anti smoke tree blight medicament is bacillus subtilis solution, the second anti smoke tree blight medicament is prochloraz solution or carbendazim solution.

In one embodiment, the concentration of Bacillus subtilis in the Bacillus subtilis solution is between 0.17 and 0.25 billion spores/mL.

In one embodiment, the concentration of the prochloraz active ingredient in the prochloraz solution is 0.450 mg/mL-0.563 mg/mL.

In one embodiment, the concentration of the carbendazim component in the carbendazim solution is 0.50mg/mL to 0.625 mg/mL.

In one embodiment, the first anti-cotinus coggygria wilt agent is a bacillus subtilis solution and the second anti-cotinus coggygria wilt agent is a carbendazim solution.

In one embodiment, the step of applying the first agent to the roots of the cotinus coggygria plant is applying the first agent to the roots of the cotinus coggygria plant 2 times at a dose of from 400mL/cm (trunk ground diameter) to 600mL/cm (trunk ground diameter).

In one embodiment, the time interval between two consecutive administrations is from 10 days to 15 days.

In one embodiment, the step of injecting the second anti-cotinus coggygria wilt agent into the trunk of the cotinus coggygria plant comprises injecting the second anti-cotinus coggygria wilt agent into the trunk of the cotinus coggygria plant for 2-5 times, wherein the dosage applied in each time is 200mL/cm (trunk ground diameter) to 250mL/cm (trunk ground diameter).

In one embodiment, the time interval between two consecutive administrations is from 10 days to 15 days.

In one embodiment, the injection of the second anti-cotinus coggygria wilt agent into the trunk of the cotinus coggygria plant is at a location of 0-80 cm from the branch-branching end of the diseased branch.

Compared with the prior art, the invention has at least the following beneficial effects:

the method for preventing and treating the cotinus coggygria fusarium wilt provided by the invention adopts a comprehensive prevention and treatment method combining root application and trunk injection to prevent and treat the cotinus coggygria fusarium wilt, on one hand, the root irrigation of bacillus subtilis is adopted to reduce the quantity of verticillium dahliae and microcolonies in soil, the infection of pathogenic bacteria to the cotinus coggygria root is greatly weakened, and meanwhile, the trunk injection is adopted to convey prochloraz or carbendazim to cotinus coggygria vascular bundle, so that the damage and blockage of the pathogenic bacteria to the cotinus coggygria tree body are weakened, and the absorption of water and mineral substances tends to be normal. The method can treat both principal and secondary aspects of diseases, and the combined use of the bacillus subtilis and the prochloraz or the bacillus subtilis and the carbendazim has better drug effect and more effective prevention and treatment effect on the smoke tree woodland blight. Compared with a single prevention and treatment method, the method for preventing and treating the cotinus coggygria fusarium wilt has better prevention and treatment effect, obviously reduces the relative disease index, more effectively reduces the damage of the cotinus coggygria fusarium wilt to the cotinus coggygria, and improves the ornamental value and the ecological value of the cotinus coggygria.

Detailed Description

Reference will now be made in detail to embodiments of the invention, one or more examples of which are described below. Each example is provided by way of explanation, not limitation, of the invention. In fact, it will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the scope or spirit of the invention. For instance, features illustrated or described as part of one embodiment, can be used on another embodiment to yield a still further embodiment.

It is therefore intended that the present invention cover the modifications and variations of this invention provided they come within the scope of the appended claims and their equivalents. Other objects, features and aspects of the present invention are disclosed in or are apparent from the following detailed description. It is to be understood by one of ordinary skill in the art that the present discussion is a description of exemplary embodiments only, and is not intended as limiting the broader aspects of the present invention.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

The terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature.

Other than as shown in the operating examples, or where otherwise indicated, all numbers expressing quantities of ingredients, physical and chemical properties, and so forth used in the specification and claims are to be understood as being modified in all instances by the term "about". For example, unless indicated to the contrary, the numerical parameters set forth in the foregoing specification and attached claims are approximations that can be suitably varied by those skilled in the art in seeking to obtain the desired properties utilizing the teachings disclosed herein. The use of numerical ranges by endpoints includes all numbers within that range and any range within that range, for example, 1 to 5 includes 1, 1.1, 1.3, 1.5, 2, 2.75, 3, 3.80, 4, and 5, and the like.

The embodiment of the invention provides a method for preventing and treating cotinus coggygria wilt, which comprises the following steps:

applying a first agent against cotinus coggygria blight to the roots of the cotinus coggygria plant; and

injecting a second cotinus coggygria blast resistant agent into the trunk of the cotinus coggygria plant;

the first anti smoke tree blight medicament is bacillus subtilis solution, the second anti smoke tree blight medicament is prochloraz solution or carbendazim solution.

In some embodiments, the concentration of bacillus subtilis in the bacillus subtilis solution is any value between 0.17 and 0.25 billion spores/mL. The bacillus subtilis solution can be obtained by diluting various medicament forms such as bacillus subtilis wettable powder, bacillus subtilis suspending agent, bacillus subtilis aqueous emulsion, bacillus subtilis water dispersible granules or bacillus subtilis granules with water. In some embodiments, the bacillus subtilis aqueous suspension may be prepared by mixing bacillus subtilis wettable powder and water in a mass ratio of 1: (400-600), wherein the content of the bacillus subtilis in the bacillus subtilis wettable powder is 100 hundred million spores/g.

In some embodiments, the concentration of the prochloraz active ingredient in the prochloraz solution is any value between 0.450mg/mL and 0.563 mg/mL. The prochloraz solution can be obtained by diluting various medicament forms such as prochloraz wettable powder, prochloraz suspending agent, prochloraz aqueous emulsion, prochloraz water dispersible granules or prochloraz granules with water. In some embodiments, the prochloraz solution is prepared from a prochloraz aqueous emulsion and water in a mass ratio of 1: (800-1000), wherein the mass ratio of the active components of the prochloraz in the prochloraz aqueous emulsion is 45%.

In some embodiments, the concentration of the carbendazim component in the carbendazim solution is any value between 0.50mg/mL and 0.625 mg/mL. The carbendazim solution can be obtained by diluting various medicament forms such as carbendazim wettable powder, carbendazim suspending agent, carbendazim water emulsion, carbendazim water dispersible granule or carbendazim granule and the like with water. In some embodiments, the carbendazim solution is formed by mixing carbendazim wettable powder and water in a mass ratio of 1 (800-1000), and the mass percentage content of the carbendazim flexible ingredient in the carbendazim wettable powder is 50%.

In some more preferred embodiments, said first anti-cotinus coggygria wilt agent is a bacillus subtilis solution and said second anti-cotinus coggygria wilt agent is a carbendazim solution. The damage of the blight to the cotinus coggygria can be effectively reduced by applying the bacillus subtilis at the root and combining a pesticide application combination mode of injecting carbendazim into the trunk.

In some embodiments, the step of applying the first agent to the roots of the cotinus coggygria plant is applying the first agent to the roots of the cotinus coggygria plant 2 times at a dose of from 400mL/cm (trunk ground diameter) to 600mL/cm (trunk ground diameter).

Preferably, the first agent is administered to the roots of the cotinus coggygria plant at a time interval of between 10 days and 15 days between successive administrations.

In some embodiments, the step of injecting the second anti-cotinus coggygria blast agent into the trunk of the cotinus coggygria plant is injecting the second anti-cotinus coggygria blast agent into the trunk of the cotinus coggygria plant 2 times at a dose of 200g/cm (trunk diameter) to 250g/cm (trunk diameter).

Preferably, the time interval between two consecutive administrations is 10 to 15 days when the second smoke tree blight resistant agent is injected into the trunk of the smoke tree plant.

Preferably, the injection position of the second smoke tree blight resistant agent to the trunk of the smoke tree plant is 0-80 cm away from the branch branching end of the diseased branch. The distance is a vertical distance relative to the ground.

Generally, the diameter of the tree trunk of the cotinus coggygria plant is more than 10 cm.

The method for controlling cotinus coggygria wilt of the present invention will be described in further detail with reference to the following examples. The present invention is intended to be further described in detail to assist those skilled in the art and researchers to further understand the present invention, and the technical conditions and the like do not limit the present invention. Any modification made within the scope of the claims of the present invention is within the scope of the claims of the present invention. The examples, which are not specifically illustrated, employ drugs and equipment, all of which are conventional in the art. The experimental procedures, in which specific conditions are not indicated in the examples, were carried out according to conventional conditions, such as those described in the literature, in books, or as recommended by the manufacturer.

The test site is located in a cotinus coggygria planting area in a Shanshan park in Beijing, the test site is a cotinus coggygria planting area which is green and is located in the east of the Jing Cui lake of Shanshan, is located above the ridge, has the altitude of 125-200 m, slopes towards the northeast, the slopes are 3-30 degrees, the stand type is a cotinus coggygria artificial mixed forest, and the cotinus coggygria with different ages exist.

Before thawing to germination in spring, applying Yufeng 99 plant protection in soil around the cotinus coggygria planting field (except for applying Yufeng 99 plant protection, other fertilizers capable of increasing the growth vigor of cotinus coggygria plants are all suitable for the application of the fertilizer, and the fertilizer has the function of improving the growth vigor of the plants, promoting the growth of branches and leaves of the cotinus coggygria and enhancing the growth vigor of the branches and leaves of the cotinus coggygria.

Example 1

1. Preparing anti-blight medicament solution

(1) Adding bacillus subtilis suspension (100 hundred million spores/gram) into water, mixing with the water, stirring for dissolving, and uniformly dispersing to prepare the bacillus subtilis aqueous suspension, namely preparing the bacillus subtilis aqueous suspension with the weight ratio of 500 to 500, wherein the weight ratio of the bacillus subtilis aqueous suspension to the water is 1: 500.

(2) Adding 45% of prochloraz aqueous emulsion into water, mixing with the water, stirring for dissolving, and uniformly dispersing to prepare 800 times of prochloraz diluent, wherein the weight ratio of the 45% of prochloraz aqueous emulsion to the water is 1: 800.

2. Processing method

50 sick cotinus coggygria with similar tree height, age and growth vigor are selected in a test field and divided into 4 treatment groups and 1 control group, wherein each group comprises 10 trees. The treatment with chemicals was started for each of the 4 treatment groups in 8 months of 2020.

(1) Irrigating roots in 8 last ten days, and performing root irrigation for 2 times in total, wherein the time interval between the root irrigation for the 1 st time and the root irrigation for the 2 nd time is 10 days. The root irrigation agent is selected from 500-time aqueous suspension of bacillus subtilis prepared in step 1, the dosage of the agent is 500mL of the 500-time aqueous suspension of bacillus subtilis applied to each centimeter according to the ground diameter of a trunk, namely the dosage of the agent is 500mL/cm (the ground diameter of the trunk) of each tree, namely the dosage of the 500-time aqueous suspension of bacillus subtilis applied to each time is 500mL if the ground diameter of a cotinus coggygria trunk is 1cm, and the dosage of the 500-time aqueous suspension of bacillus subtilis applied to each time is 5000mL if the ground diameter of the cotinus coggygria trunk is 10 cm. Generally, the diameter of the trunk is larger than 10 cm.

(3) Injecting the pesticide into the trunk in the last ten days of 9 months, filling 800 times of the prochloraz diluent into a tree infusion bag, injecting the pesticide into the trunk base which is about 80cm away from the lower end of the bifurcation of the diseased branch, adopting a hanging needle method, injecting one bag of pesticide for about 10 days at a time, and replacing the other bag after the injection is finished for 5 times. The injection amount of the drug is 200mL of prochloraz 800 times diluent per centimeter of the ground diameter of a trunk, namely, the injection dose per tree is 200mL/cm (the ground diameter of the trunk), namely if the ground diameter of the cotinus coggygria trunk is 1cm, the injection amount per time of prochloraz 800 times diluent is 200mL, and if the ground diameter of the cotinus coggygria trunk is 10cm, the injection amount per time of prochloraz 800 times diluent is 2000 mL. Generally, the diameter of the trunk is larger than 10 cm.

Cotinus coggygria plants without any treatment were used as a placebo.

Example 2

1. Preparing anti-blight medicament solution

(1) Adding bacillus subtilis suspension (100 hundred million spores/gram) into water, mixing with the water, stirring for dissolving, and uniformly dispersing to prepare the bacillus subtilis aqueous suspension, namely preparing the bacillus subtilis aqueous suspension with the weight ratio of 500 to 500, wherein the weight ratio of the bacillus subtilis aqueous suspension to the water is 1: 500.

(2) Adding 50% carbendazim wettable powder into water, mixing with water, stirring for dissolving, and dispersing uniformly to prepare 800 times of carbendazim diluent, wherein the weight ratio of 50% carbendazim to water is 1: 800.

2. Processing method

50 sick cotinus coggygria with similar tree height, age and growth vigor are selected in a test field and divided into 4 treatment groups and 1 control group, wherein each group comprises 10 trees. The treatment with chemicals was started for each of the 4 treatment groups in 8 months of 2020.

(1) Irrigating roots in 8 last ten days, and performing root irrigation for 2 times in total, wherein the time interval between the root irrigation for the 1 st time and the root irrigation for the 2 nd time is 15 days. The root irrigation agent is selected from 500-fold aqueous suspension of bacillus subtilis prepared in step 1, and the dosage of the agent is 500mL of 500-fold aqueous suspension of bacillus subtilis applied per centimeter of the ground diameter of the tree trunk, namely the dosage of 500mL/cm (the ground diameter of the tree trunk) of each tree, namely 500mL of 500-fold aqueous suspension applied per time if the ground diameter of the tree trunk of the cotinus coggygria is 1cm, and 5000mL of 500-fold aqueous suspension applied per time if the ground diameter of the tree trunk of the cotinus coggygria is 10 cm. Generally, the diameter of the trunk is larger than 10 cm.

(2) Injecting the trunk with the pesticide in the last ten days of 9 months, filling 800 times of the carbendazim diluent into a tree infusion bag, injecting the trunk base which is about 80cm away from the lower end of the bifurcation of the diseased branch, adopting a method of hanging a hanging needle, injecting one bag of pesticide for about 10 days, and replacing the other bag after the injection is finished for 5 times. 200mL of carbendazim 800-time diluent is injected into each centimeter of trunk diameter of the medicament, namely the injection dosage of each tree is 200mL/cm (trunk diameter), namely if the diameter of the cotinus coggygria trunk is 1cm, the injection dosage of the carbendazim 800-time diluent is 200mL, and if the diameter of the cotinus coggygria trunk is 10cm, the injection dosage of the carbendazim 800-time diluent is 2000 mL. Generally, the diameter of the trunk is larger than 10 cm.

Cotinus coggygria plants without any treatment were used as a placebo.

Comparative example 1

1. Preparing anti-blight medicament solution

Adding bacillus subtilis suspension (100 hundred million spores/gram) into water, mixing with the water, stirring for dissolving, and uniformly dispersing to prepare the bacillus subtilis aqueous suspension, namely preparing the bacillus subtilis aqueous suspension with the weight ratio of 500 to 500, wherein the weight ratio of the bacillus subtilis aqueous suspension to the water is 1: 500.

2. Processing method

80 diseased cotinus coggygria with similar tree height, age and growth vigor are selected in a test field and divided into 2 treatment groups and 1 control group, wherein each group comprises 20 trees. The treatment of 2 treatment groups with the medicament was started in 8 months of 2020.

Irrigating roots in 8 last ten days, and performing root irrigation for 2 times in total, wherein the time interval between the root irrigation for the 1 st time and the root irrigation for the 2 nd time is 15 days. The root irrigation agent is selected from 500 times of aqueous suspension of bacillus subtilis prepared in the step 1, the dosage of the agent is 500g of the aqueous suspension of the bacillus subtilis applied to each centimeter according to the ground diameter of a trunk, namely the dosage of the agent is 500g/cm (the ground diameter of the trunk) for each tree, namely the dosage of 500g of the aqueous suspension of the bacillus subtilis applied to each time is 500g if the ground diameter of the cotinus coggygria trunk is 1cm, and the dosage of 500g of the aqueous suspension of the bacillus subtilis applied to each time is 5000g if the ground diameter of the cotinus coggygria trunk is 10 cm. Generally, the diameter of the trunk is larger than 10 cm.

Cotinus coggygria plants without any treatment were used as a placebo.

Comparative example 2

1. Preparing anti-blight medicament solution

Adding 25% of azoxystrobin suspending agent into water, mixing with water, stirring for dissolving, and dispersing uniformly to prepare azoxystrobin 500 times of diluent, wherein the weight ratio of the 25% of azoxystrobin suspending agent to water is 1: 500.

2. Processing method

Comparative example 2 the treatment method was substantially the same as in comparative example 1, except that the 500-fold aqueous suspension of the drug bacillus subtilis was replaced with 500-fold diluent of azoxystrobin, and the application rate was unchanged.

Cotinus coggygria plants without any treatment were used as a placebo.

Comparative example 3

1. Preparing anti-blight medicament solution

Adding 50% carbendazim wettable powder into water, mixing with water, stirring for dissolving, and dispersing uniformly to prepare 800 times of carbendazim diluent, wherein the weight ratio of 50% carbendazim to water is 1: 800.

2. Processing method

80 diseased cotinus coggygria with similar tree height, age and growth vigor are selected in a test field and divided into 2 treatment groups and 1 control group, wherein each group comprises 20 trees. The treatment of 2 treatment groups with the medicament was started in the first 9 th of 2020.

Filling the dilution of 800 times of 50% carbendazim into a tree infusion bag, injecting at the base of a trunk which is about 80cm away from the lower end of the bifurcation of a diseased branch, and continuously injecting to the bottom of 10 months. The continuous injection is that one bag of medicine can be injected for about 10 days by adopting a method of hanging a needle, and the other bag is replaced after the injection is finished. The total injection amount of the medicament is 200g of carbendazim 800-time diluent per centimeter of the ground diameter of a trunk, namely, the injection dose is 200g/cm (the ground diameter of the trunk) each time, namely, if the ground diameter of the cotinus coggygria trunk is 1cm, the injection dose of the carbendazim 800-time diluent per time is 200g, and if the ground diameter of the cotinus coggygria trunk is 10cm, the injection dose of the carbendazim 800-time diluent per time is 2000 g. Generally, the diameter of the trunk is larger than 10 cm.

Cotinus coggygria plants without any treatment were used as a placebo.

Comparative example 4

1. Preparing anti-blight medicament solution

Adding 45% of prochloraz aqueous emulsion into water, mixing with the water, stirring for dissolving, and uniformly dispersing to prepare 800 times of prochloraz diluent, wherein the weight ratio of the 45% of prochloraz aqueous emulsion to the water is 1: 800.

2. Processing method

Comparative example 4 the treatment method was substantially the same as in comparative example 3, except that 800-fold dilution of the injection carbendazim was replaced with 800-fold dilution of prochloraz, and the application rate was unchanged.

Cotinus coggygria plants without any treatment were used as a placebo.

Comparative example 5

1. Preparing anti-blight medicament solution

(1) Adding 25% of azoxystrobin suspending agent into water, mixing with water, stirring for dissolving, and dispersing uniformly to prepare azoxystrobin 500 times of diluent, wherein the weight ratio of the 25% of azoxystrobin suspending agent to water is 1: 500.

(2) Adding 45% of prochloraz aqueous emulsion into water, mixing with the water, stirring for dissolving, and uniformly dispersing to prepare 800 times of prochloraz diluent, wherein the weight ratio of the 45% of prochloraz aqueous emulsion to the water is 1: 800.

2. Processing method

50 sick cotinus coggygria with similar tree height, age and growth vigor are selected in a test field and divided into 4 treatment groups and 1 control group, wherein each group comprises 10 trees. The treatment with chemicals was started for each of the 4 treatment groups in 8 months of 2020.

(1) Irrigating roots in 8 last ten days, and performing root irrigation for 2 times in total, wherein the time interval between the root irrigation for the 1 st time and the root irrigation for the 2 nd time is 10 days. The root irrigation agent is selected from the azoxystrobin 500-fold diluent prepared in the step 1, the using amount of the agent is 500mL of the azoxystrobin 500-fold diluent applied to each centimeter according to the ground diameter of a trunk, namely the using amount of the agent is 500mL/cm (the ground diameter of the trunk) for each tree, namely if the ground diameter of a cotinus coggygria trunk is 1cm, the using amount of the azoxystrobin 500-fold diluent applied to each time is 500mL, and if the ground diameter of the cotinus coggygria trunk is 10cm, the using amount of the agent is 5000 mL. Generally, the diameter of the trunk is larger than 10 cm.

(2) Injecting the pesticide into the trunk in the last ten days of 9 months, filling 800 times of the prochloraz diluent into a tree infusion bag, injecting the pesticide into the trunk base which is about 80cm away from the lower end of the bifurcation of the diseased branch, adopting a hanging needle method, injecting one bag of pesticide for about 10 days at a time, and replacing the other bag after the injection is finished for 5 times. The injection amount of the drug is 200mL of prochloraz 800 times diluent per centimeter of the ground diameter of a trunk, namely, the injection dose per tree is 200mL/cm (the ground diameter of the trunk), namely if the ground diameter of the cotinus coggygria trunk is 1cm, the injection amount per time of prochloraz 800 times diluent is 200mL, and if the ground diameter of the cotinus coggygria trunk is 10cm, the injection amount per time of prochloraz 800 times diluent is 2000 mL. Generally, the diameter of the trunk is larger than 10 cm.

Cotinus coggygria plants without any treatment were used as a placebo.

Comparative example 6

1. Preparing anti-blight medicament solution

(1) Adding 25% of azoxystrobin suspending agent into water, mixing with water, stirring for dissolving, and dispersing uniformly to prepare azoxystrobin 500 times of diluent, wherein the weight ratio of the 25% of azoxystrobin suspending agent to water is 1: 500.

(2) Adding 50% carbendazim wettable powder into water, mixing with water, stirring for dissolving, and dispersing uniformly to prepare 800 times of carbendazim diluent, wherein the weight ratio of 50% carbendazim to water is 1: 800.

2. Processing method

50 sick cotinus coggygria with similar tree height, age and growth vigor are selected in a test field and divided into 4 treatment groups and 1 control group, wherein each group comprises 10 trees. The treatment with chemicals was started for each of the 4 treatment groups in 8 months of 2020.

(1) Irrigating roots in 8 last ten days, and performing root irrigation for 2 times in total, wherein the time interval between the root irrigation for the 1 st time and the root irrigation for the 2 nd time is 15 days. The drug for root irrigation is 500-fold diluent of azoxystrobin prepared in step 1, the drug dosage is 500mL of azoxystrobin 500-fold diluent applied to each centimeter according to the ground diameter of a trunk, namely the drug dosage of each tree is 500mL/cm (the ground diameter of the trunk), namely if the ground diameter of a cotinus coggygria trunk is 1cm, the amount of azoxystrobin 500-fold diluent applied to each tree is 500mL, and if the ground diameter of the cotinus coggygria trunk is 10cm, the amount of azoxystrobin 500-fold diluent applied to each tree is 5000 mL. Generally, the diameter of the trunk is larger than 10 cm.

(2) And (3) in the last ten days of 9 months, filling 800 times of diluent of carbendazim into a tree infusion bag, injecting at the base of the trunk which is about 80cm away from the lower end of the branch of the diseased branch, and continuously injecting to the bottom of 10 months. The continuous injection is that one bag of medicine can be injected for about 10 days by adopting a method of hanging a needle, and the other bag is replaced after the injection is finished. The total injection amount of the medicament is 200mL of 800-time carbendazim diluent per centimeter of the ground diameter of a trunk, namely, the injection dose per time is 200mL/cm (the ground diameter of the trunk), namely if the ground diameter of the cotinus coggygria trunk is 1cm, the injection dose per time is 200mL of 800-time carbendazim diluent, and if the ground diameter of the cotinus coggygria trunk is 10cm, the injection dose per time is 2000mL of 800-time carbendazim diluent. Generally, the diameter of the trunk is larger than 10 cm.

Cotinus coggygria plants without any treatment were used as a placebo.

The classification criteria for Cotinus coggygria blight are shown in Table 1.

TABLE 1 Cotinus coggygria blast classification criteria

After the cotinus coggygria plants of examples 1-2 and comparative examples 5-6 are subjected to root irrigation for 10 days, the severity of the disease of the cotinus coggygria is investigated and recorded, the cotinus coggygria is investigated once every 10 days for 3 times, the disease index, the prevention and treatment effect and the relative prevention and treatment effect are calculated, and statistical analysis is carried out by applying SPASS 26.0 software. Wherein the disease index, the prevention and treatment effect and the relative prevention and treatment effect are calculated according to the following formulas, and the investigation and calculation results are shown in table 2.

Relative control effect-control effect of control group

TABLE 2

After the cotinus coggygria plants in the comparative examples 1 and 2 are applied for 10 days after root irrigation for the second time, the severity of the disease of the cotinus coggygria is investigated and recorded, 1 investigation is carried out every 10 days, 3 investigations are carried out totally, the disease index, the prevention and treatment effect and the relative prevention and treatment effect are calculated, and statistical analysis is carried out by applying SPASS 26.0 software. Wherein the disease index, the prevention and treatment effect and the relative prevention and treatment effect are calculated according to the above formula, and the investigation and calculation results are shown in table 3.

TABLE 3

The cotinus coggygria plants of the comparative example 3 and the comparative example 4 started to investigate and record the severity of the onset of the cotinus coggygria after 20 days of injection, were investigated 3 times every 10 days, and the disease index, the prevention and treatment effect and the relative prevention and treatment effect were calculated, and statistical analysis was performed using the spars 26.0 software. Wherein the disease index, the prevention and treatment effect and the relative prevention and treatment effect are calculated according to the above formula, and the investigation and calculation results are shown in table 4.

TABLE 4

Therefore, the method for preventing and treating the cotinus coggygria fusarium wilt has better prevention and treatment effects.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present invention, so as to understand the technical solutions of the present invention specifically and in detail, but not to be understood as the limitation of the protection scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. It should be understood that the technical solutions provided by the present invention, which are obtained by logical analysis, reasoning or limited experiments, are within the scope of the appended claims. Therefore, the protection scope of the patent of the invention is subject to the content of the appended claims, and the description can be used for explaining the content of the claims.