阅读说明：本技术 一种微生物和植物共生的营养基质配方及制备工艺 (Formula and preparation process of nutrient medium for symbiosis of microorganisms and plants ) 是由 郑殿峰 冯乃杰 沈雪峰 赵黎明 母德伟 余明龙 于 2021-09-17 设计创作，主要内容包括：本发明公开了一种微生物和植物共生的营养基质配方,由以下材料的重量份的成分组成：磷酸10g-30g、硫酸钾10g-30g、硫酸锌10g-30g、尿素10g-30g、木质素苯磺酸钙10g-30g、氯化钠10g-30g、氨水10－30g、7水硫酸镁10g-30g、7水硫酸亚铁10g-30g、农用氨基酸10g-30g、葡萄糖10g-30g、褐煤20g-100g、麦饭石20g-100g、沸石粉20g-100g、磷石膏20g-100g、腐殖土20g-100g和硅藻土20g-100g。该微生物和植物共生的营养基的制备工艺,能够用于筛选在土壤中活性强,对作物生长发育有益的细菌、放线菌和真菌等微生物,也可以检测现有微生物肥料的有效性,主要利用微生物和植物可以共生的基质,添加微生物和植物生长所必须的营养物质,制备可以同时培养微生物和植物,研究微生物及其代谢物对植物生长发育的影响的共同培养基质。(The invention discloses a nutrient substrate formula for symbiosis of microorganisms and plants, which comprises the following components in parts by weight: 10-30 g of phosphoric acid, 10-30 g of potassium sulfate, 10-30 g of zinc sulfate, 10-30 g of urea, 10-30 g of calcium lignosulphonate, 10-30 g of sodium chloride, 10-30 g of ammonia water, 10-30 g of 7-water magnesium sulfate, 10-30 g of 7-water ferrous sulfate, 10-30 g of agricultural amino acid, 10-30 g of glucose, 20-100 g of lignite, 20-100 g of medical stone, 20-100 g of zeolite powder, 20-100 g of phosphogypsum, 20-100 g of humus and 20-100 g of diatomite. The preparation process of the microbial and plant symbiotic nutrient medium can be used for screening microbes such as bacteria, actinomycetes and fungi which have strong activity in soil and are beneficial to the growth and development of crops, and can also be used for detecting the effectiveness of the existing microbial fertilizer.)

1. A nutrient substrate formula for symbiosis of microorganisms and plants is characterized by comprising the following materials in parts by weight: 10-30 g of phosphoric acid, 10-30 g of potassium sulfate, 10-30 g of zinc sulfate, 10-30 g of urea, 10-30 g of calcium lignosulphonate, 10-30 g of sodium chloride, 10-30 g of ammonia water, 10-30 g of 7-water magnesium sulfate, 10-30 g of 7-water ferrous sulfate, 10-30 g of agricultural amino acid, 10-30 g of glucose, 20-100 g of lignite, 20-100 g of medical stone, 20-100 g of zeolite powder, 20-100 g of phosphogypsum, 20-100 g of humus and 20-100 g of diatomite.

2. The microbial and plant symbiotic nutrient medium formula according to claim 1, characterized by comprising the following components in parts by weight: 30-60 g of phosphoric acid, 30-60 g of potassium sulfate, 30-60 g of zinc sulfate, 30-60 g of urea, 30-60 g of calcium lignosulphonate, 30-60 g of sodium chloride, 30-60 g of ammonia water, 30-60 g of 7-water magnesium sulfate, 30-60 g of 7-water ferrous sulfate, 30-60 g of agricultural amino acid, 30-60 g of glucose, 40-140 g of lignite, 40-140 g of medical stone, 40-140 g of zeolite powder, 40-140 g of phosphogypsum, 40-140 g of humus soil and 40-140 g of diatomite.

3. The microbial and plant symbiotic nutrient medium formula according to claim 1, characterized by comprising the following components in parts by weight: 40-70 g of phosphoric acid, 40-70 g of potassium sulfate, 40-70 g of zinc sulfate, 40-70 g of urea, 40-70 g of calcium lignosulphonate, 40-70 g of sodium chloride, 40-70 g of ammonia water, 40-70 g of 7-water magnesium sulfate, 40-70 g of 7-water ferrous sulfate, 40-70 g of agricultural amino acid, 40-70 g of glucose, 60-280 g of lignite, 60-280 g of medical stone, 60-280 g of zeolite powder, 60-280 g of phosphogypsum, 60-280 g of humus soil and 60-280 g of diatomite.

4. The microbial and plant symbiotic nutrient medium formula according to claim 1, characterized by comprising the following components in parts by weight: 50-85 g of phosphoric acid, 50-85 g of potassium sulfate, 50-85 g of zinc sulfate, 50-85 g of urea, 50-85 g of calcium lignosulphonate, 50-85 g of sodium chloride, 50-85 g of ammonia water, 50-85 g of 7-water magnesium sulfate, 50-85 g of 7-water ferrous sulfate, 50-85 g of agricultural amino acid, 50-85 g of glucose, 80-350 g of lignite, 80-350 g of medical stone, 80-350 g of zeolite powder, 80-350 g of phosphogypsum, 80-350 g of humus soil and 80-350 g of diatomite.

5. The microbial and plant symbiotic nutrient medium formula according to claim 1, characterized by comprising the following components in parts by weight: 60-100 g of phosphoric acid, 60-100 g of potassium sulfate, 60-100 g of zinc sulfate, 60-100 g of urea, 60-100 g of calcium lignosulphonate, 60-100 g of sodium chloride, 60-100 g of ammonia water, 60-100 g of 7-water magnesium sulfate, 60-100 g of 7-water ferrous sulfate, 60-100 g of agricultural amino acid, 60-100 g of glucose, 100-400 g of lignite, 100-400 g of medical stone, 100-400 g of zeolite powder, 100-400 g of phosphogypsum, 100-400 g of humus soil and 100-400 g of diatomite.

6. A microbial and plant symbiotic nutritional matrix formulation according to claim 1, characterized in that: the pH value of the microbial and plant symbiotic nutrient medium is 5.0-7.5 (depending on the pH value of soil to be applied).

7. The process for preparing a formulation of a microbiologically and phytosymbiotic nutritional substrate according to any one of claims 1 to 6, comprising the following specific steps:

(1) adding 1g-100g of ammonia water in each Kg of nutrient medium symbiotic with microorganisms and plants into 10g-400g of lignite, stirring, airing and grinding.

(2) Adding 1g-100g of urea in each Kg of nutrient medium symbiotic with the microorganisms and the plants into 1g-100g of phosphoric acid to generate chemical reaction to generate urea phosphate which can be commonly utilized by the plants and the microorganisms, stirring, airing and grinding.

(3) And (3) mixing the products obtained in the step (1) and the step (2) with the rest substances, and uniformly stirring to obtain the nutrient substrate for symbiosis of microorganisms and plants.

(4) Taking a certain amount of nutrient medium symbiotic with microorganisms and plants, putting into a container such as a bowl, and sterilizing for later use.

(5) Soaking the plant seeds in 0.1 percent potassium permanganate solution for 10 minutes for disinfection, and then sucking the plant seeds with sterile paper for later use.

(6) Digging a nutrient medium at the center of the pot on a clean bench by using a sterile spoon for 4 cm, inoculating microorganisms to be researched or inspected, covering the medium for 1 cm, sowing sterilized seeds, and covering the medium for 3 cm.

(7) Placing the cultivation pot for inoculation and sowing in an aseptic or ventilated bright environment, pouring sterile water, keeping the relative water content at 70-80%, and culturing.

8. The process for preparing a formulation of nutrient medium symbiotic with microorganisms and plants according to claim 7, wherein the lignite obtained in (1) is ground and stirred with ammonia water by using an integrated filtering, ventilating and stirring device, the drying temperature of the ground and stirred lignite is set to 60 ℃, and the drying place is set inside a special drying room.

9. The process for preparing a microbially and phytosymbiotic nutritional matrix formulation according to claim 7, wherein: the temperature of the urea and the phosphoric acid in the step (2) is set to 80 ℃ during mixing, stirring and grinding, the urea and the phosphoric acid need to be dried on the inner side of a drying room at the temperature of 65 ℃ after chemical reaction, and after the nutrient substrate formed by symbiosis of microorganisms and plants is generated, an aseptic container is used for placing, and a professional sterilization instrument is used for performing sterilization treatment.

10. The process for preparing a microbially and phytosymbiotic nutritional matrix formulation according to claim 7, wherein: the cultivation pot for inoculating and sowing the bacteria is made of ceramic, is placed in a cultivation base at the temperature of 18-25 ℃, and needs to be irrigated with sterile water in a four-side ventilated environment.

Technical Field

The invention relates to the technical field of nutrient medium formulas, in particular to a nutrient medium formula for symbiosis of microorganisms and plants, a preparation process and a preparation method thereof.

Background

As for soil, the soil is a component of the nature, the inside of the soil contains certain moisture, and the moisture in the soil can play a circulating function through the soil, the soil mainly comprises three substances of solid, liquid and gas, wherein the solid substances comprise mineral substances, microorganisms, organic matters and the like of the soil, the liquid is the moisture in the soil, and the gas is mainly air shuttled between soil gaps, along with the development of science and technology, people research and analyze the soil of crops, and due to the huge pollution of air by the chemical industry, the microorganisms such as bacteria, actinomycetes, fungi and the like in the soil are in an unbalanced state, so researchers improve the soil through the nutrient medium symbiotic with the microorganisms and plants, but the existing nutrient medium symbiotic with the microorganisms and the plants still has defects in the preparation process, such as:

the preparation of the existing nutrient medium is inconvenient for simultaneously culturing microorganisms and plants and for co-culturing the medium under the influence of the microorganisms and the metabolites thereof on the growth and development of the plants, so that scientific researchers usually need to separately culture the microorganisms and the plants, and the scientific researchers are inconvenient for exactly knowing and analyzing, and the problem that some microorganisms such as bacteria, actinomycetes, fungi and the like which are lacked in the normal growth process of the plants are not beneficial to the cultivation and growth of the plants is solved;

in order to solve the above problems, innovative design based on the original polymer material is urgently needed.

Disclosure of Invention

The invention aims to provide a formula of a nutrient substrate for symbiosis of microorganisms and plants, a preparation process and a preparation method thereof, and aims to solve the problems that the preparation of the nutrient substrate in the current market is inconvenient for simultaneous culture of the microorganisms and the plants, and is inconvenient for joint culture of the influence of the microorganisms and the metabolites thereof on the growth and development of the plants, so that researchers usually need to separately culture the microorganisms and the plants, the researchers are inconvenient to exactly understand and analyze, and the microorganisms such as bacteria, actinomycetes, fungi and the like which are lacking in the normal growth process of the plants are not beneficial to the cultivation and growth of the plants.

In order to achieve the purpose, the invention provides the following technical scheme: a nutrient medium formula for symbiosis of microorganisms and plants comprises the following components in parts by weight: 10-30 g of phosphoric acid, 10-30 g of potassium sulfate, 10-30 g of zinc sulfate, 10-30 g of urea, 10-30 g of calcium lignosulphonate, 10-30 g of sodium chloride, 10-30 g of ammonia water, 10-30 g of 7-water magnesium sulfate, 10-30 g of 7-water ferrous sulfate, 10-30 g of agricultural amino acid, 10-30 g of glucose, 20-100 g of lignite, 20-100 g of medical stone, 20-100 g of zeolite powder, 20-100 g of phosphogypsum, 20-100 g of humus and 20-100 g of diatomite.

Preferably, the formula of the nutrient substrate for symbiosis of microorganisms and plants comprises the following components in parts by weight: 30-60 g of phosphoric acid, 30-60 g of potassium sulfate, 30-60 g of zinc sulfate, 30-60 g of urea, 30-60 g of calcium lignosulphonate, 30-60 g of sodium chloride, 30-60 g of ammonia water, 30-60 g of 7-water magnesium sulfate, 30-60 g of 7-water ferrous sulfate, 30-60 g of agricultural amino acid, 30-60 g of glucose, 40-140 g of lignite, 40-140 g of medical stone, 40-140 g of zeolite powder, 40-140 g of phosphogypsum, 40-140 g of humus soil and 40-140 g of diatomite.

Preferably, the formula of the nutrient substrate for symbiosis of microorganisms and plants comprises the following components in parts by weight: 40-70 g of phosphoric acid, 40-70 g of potassium sulfate, 40-70 g of zinc sulfate, 40-70 g of urea, 40-70 g of calcium lignosulphonate, 40-70 g of sodium chloride, 40-70 g of ammonia water, 40-70 g of 7-water magnesium sulfate, 40-70 g of 7-water ferrous sulfate, 40-70 g of agricultural amino acid, 40-70 g of glucose, 60-280 g of lignite, 60-280 g of medical stone, 60-280 g of zeolite powder, 60-280 g of phosphogypsum, 60-280 g of humus soil and 60-280 g of diatomite.

Preferably, the formula of the nutrient substrate for symbiosis of microorganisms and plants comprises the following components in parts by weight: 50-85 g of phosphoric acid, 50-85 g of potassium sulfate, 50-85 g of zinc sulfate, 50-85 g of urea, 50-85 g of calcium lignosulphonate, 50-85 g of sodium chloride, 50-85 g of ammonia water, 50-85 g of 7-water magnesium sulfate, 50-85 g of 7-water ferrous sulfate, 50-85 g of agricultural amino acid, 50-85 g of glucose, 80-350 g of lignite, 80-350 g of medical stone, 80-350 g of zeolite powder, 80-350 g of phosphogypsum, 80-350 g of humus soil and 80-350 g of diatomite.

Preferably, the formula of the nutrient substrate for symbiosis of microorganisms and plants comprises the following components in parts by weight: 60-100 g of phosphoric acid, 60-100 g of potassium sulfate, 60-100 g of zinc sulfate, 60-100 g of urea, 60-100 g of calcium lignosulphonate, 60-100 g of sodium chloride, 60-100 g of ammonia water, 60-100 g of 7-water magnesium sulfate, 60-100 g of 7-water ferrous sulfate, 60-100 g of agricultural amino acid, 60-100 g of glucose, 100-400 g of lignite, 100-400 g of medical stone, 100-400 g of zeolite powder, 100-400 g of phosphogypsum, 100-400 g of humus soil and 100-400 g of diatomite.

Preferably, the microbial and plant symbiotic nutrient medium has a pH value of 5.0-7.5 (depending on the pH value of the soil to be applied).

A preparation process of a nutrient substrate formula for symbiosis of microorganisms and plants comprises the following specific steps:

(1) adding 1g-100g of ammonia water in each Kg of nutrient medium symbiotic with microorganisms and plants into 10g-400g of lignite, stirring, airing and grinding.

(2) Adding 1g-100g of urea in each Kg of nutrient medium symbiotic with the microorganisms and the plants into 1g-100g of phosphoric acid to generate chemical reaction to generate urea phosphate which can be commonly utilized by the plants and the microorganisms, stirring, airing and grinding.

(3) And (3) mixing the products obtained in the step (1) and the step (2) with the rest substances, and uniformly stirring to obtain the nutrient substrate for symbiosis of microorganisms and plants.

(4) Taking a certain amount of nutrient medium symbiotic with microorganisms and plants, putting into a container such as a bowl, and sterilizing for later use.

(5) Soaking the plant seeds in 0.1 percent potassium permanganate solution for 10 minutes for disinfection, and then sucking the plant seeds with sterile paper for later use.

(6) Digging a nutrient medium at the center of the pot on a clean bench by using a sterile spoon for 4 cm, inoculating microorganisms to be researched or inspected, covering the medium for 1 cm, sowing sterilized seeds, and covering the medium for 3 cm.

(7) Placing the cultivation pot for inoculation and sowing in an aseptic or ventilated bright environment, pouring sterile water, keeping the relative water content at 70-80%, and culturing.

Preferably, the lignite in (1) is subjected to integrated filtering and air exchange stirring equipment in a device for grinding and stirring with ammonia water, the air drying temperature of the ground and stirred lignite is set to be 60 ℃, and the air drying place is set to be inside a special drying room.

Preferably, the temperature of the urea and the phosphoric acid in the step (2) is set to 80 ℃ during mixing, stirring and grinding, the urea and the phosphoric acid need to be dried inside a drying room at the temperature of 65 ℃ after chemical reaction, and after the nutrient medium symbiotic with the microorganisms and the plants is generated, the urea and the phosphoric acid are placed in an aseptic container and sterilized by using a professional sterilization instrument.

Preferably, the cultivation pot for inoculation and seeding in (7) is made of ceramic, the cultivation pot is placed in a cultivation base with the temperature of 18-25 ℃, and the cultivation pot needs to be watered with sterile water in a four-side ventilated environment.

Compared with the prior art, the invention has the beneficial effects that: the formula and the preparation process of the nutrient medium for symbiosis of the microorganisms and the plants can be used for screening the microorganisms such as bacteria, actinomycetes, fungi and the like which have strong activity in soil and are beneficial to the growth and development of crops, and can also be used for detecting the effectiveness of the existing microbial fertilizer.