阅读说明：本技术 一种防治茶黄螨的新型化学药剂 (Novel chemical agent for preventing and treating tea yellow mites ) 是由 杨祥燕 蔡元保 于 2021-09-13 设计创作，主要内容包括：本发明涉及农药技术领域,具体涉及一种防治茶黄螨的新型化学药剂。所述新型化学药剂的有效成分由乙唑螨腈、丁氟螨酯或乙螨唑与滨蒿内酯二元复配而成。本发明新型化学药剂中成有效成分在进行复配时,表现出协同增效作用,与单剂相比,可以提高对茶黄螨的防治效果,从而可以降低农药的施用量,降低防治成本和农药残留。另外,本发明新型化学药剂可以延缓害螨抗药性的产生,在一定程度上可以保证其防治效果,进而可以延长化合物的使用寿命。(The invention relates to the technical field of pesticides, and particularly relates to a novel chemical agent for preventing and treating tea yellow mites. The effective components of the novel chemical agent are formed by binary combination of pyrazotocast ethyl, cyflumetofen or etoxazole and scoparone. The effective components in the novel chemical agent show synergistic effect when being compounded, and compared with a single agent, the novel chemical agent can improve the control effect on tea yellow mites, thereby reducing the application amount of pesticides and reducing the control cost and pesticide residues. In addition, the novel chemical agent can delay the generation of drug resistance of harmful mites, can ensure the control effect to a certain extent, and further can prolong the service life of the compound.)

1. A novel chemical agent for preventing and treating yellow tea mites is characterized in that the effective components of the novel chemical agent are formed by binary combination of pyrazotocast ethyl, cyflumetofen or etoxazole and scoparone.

2. The novel chemical agent for controlling yellow tea mites as claimed in claim 1, wherein the mass ratio of ethizole mite nitrile to scoparone is 1-40: 40-1.

3. The novel chemical agent for controlling yellow tea mites as claimed in claim 1, wherein the mass ratio of cyflumetofen to scoparone is 1-15: 60-1.

4. The novel chemical agent for controlling yellow tea mites as claimed in claim 1, wherein the mass ratio of etoxazole to scoparone is 1-20: 5-1.

Technical Field

The invention relates to the technical field of pesticides, and particularly relates to a novel chemical agent for preventing and treating tea yellow mites.

Background

Theaflavia genus Acarina order Tarsonemus family, is a polyphagic pest. Tea yellow mites generally damage macadimia nuts, and are usually deposited on the back surfaces and tender tips of leaves of macadimia nut plants by adults and nymphs, and juice of the leaves and the tender tips of the macadimia nuts is absorbed by a piercing-sucking mouthpart, so that the leaves of the macadimia nut plants are yellow, fall off, have weakened growth vigor and fall flowers and fruits, the yield and the fruit quality of the macadimia nuts are reduced, and the tea yellow mites are usually used as one of key control objects in the process of planting the macadimia nuts. Chemical agents such as 2.5 percent bifenthrin emulsifiable solution, 25 percent fenisobromolate emulsifiable solution or 20 percent dicofol emulsifiable solution are usually selected to prevent and control macadamia nut yellow mites. However, with the generation of drug resistance of yellow tea mite, the control effect of the existing chemical agents is gradually reduced, so that it is necessary to develop new chemical agents for controlling yellow tea mite.

The scoparone (scoparone) has the chemical name of 6, 7-dimethoxy coumarin, is a coumarin compound, and has better acaricidal activity. Application number CN201510777356.0 discloses the use of scoparone as acaricide, which can be used for killing tetranychus cinnabarinus, tetranychus urticae koch, panonychus citri, panonychus ulmi koch, tetranychus theobromus and tetranychus crataegi.

The chemical agents with single component are compounded, the screened compounded combination with the synergistic effect can be used for developing novel agents, and compared with the single agent, the compounded combination with the synergistic effect can improve the control effect, reduce the application amount of pesticides and is beneficial to delaying the generation of drug resistance. The inventor discovers that remarkable synergistic effect is shown when the scoparone and the difenoconazole, the cyflumetofen or the etoxazole are compounded in a certain mass ratio range through a large number of indoor activity tests, and the composition can be used for developing novel chemical agents. At present, no relevant report is found.

Disclosure of Invention

The invention aims to provide a novel chemical agent for preventing and treating yellow tea mites, which aims to solve the problem that the prevention and treatment effects of the existing chemical agent on the yellow tea mites are gradually reduced along with the generation of drug resistance of macadimia nuts.

In order to achieve the purpose, the invention provides the following technical scheme:

the effective components of the novel chemical agent are formed by binary combination of ethiprole, cyflumetofen or etoxazole and scoparone.

Preferably, the mass ratio of the ethacrylonitrile to the scoparone is 1-40: 40-1.

Preferably, the mass ratio of the cyflumetofen to the scoparone is 1-15: 60-1.

Preferably, the mass ratio of etoxazole to scoparone is 1-20: 5-1.

Compared with the prior art, the invention has the following beneficial effects:

(1) the effective components in the novel chemical agent show synergistic effect when being compounded, and compared with a single agent, the novel chemical agent can improve the control effect on tea yellow mites, thereby reducing the application amount of pesticides and reducing the control cost and pesticide residues.

(2) The novel chemical agent can delay the generation of drug resistance of pest mites, can ensure the control effect to a certain extent, and further can prolong the service life of the compound.

Detailed Description

The following detailed description of specific embodiments of the invention is provided, but it should be understood that the scope of the invention is not limited to the specific embodiments.

Examples: indoor bioactivity test after scoparone compounding

Reagent to be tested: 98% of scoparone technical, 98% of ethacrylonitrile technical, 97% of cyflumetofen technical and 96% of etoxazole technical.

For testing mites: tea yellow mites collected from macadimia nut plants were bred in laboratories using macadimia nut young leaves, and female adult mites of consistent physiological status were selected as test insects.

The test method comprises the following steps: (refer to pesticide laboratory bioassay Standard insecticide part 12: Tetranychus urticae slide dipping method)

1. Cutting the double-sided adhesive tape into 2cm long, sticking the double-sided adhesive tape to one end of a glass slide, sticking 30 heads of each glass slide on the double-sided adhesive tape, placing the glass slide into a container filled with wet sponge, covering the container with a cover, and placing the container at the temperature of (25 +/-1) ° C. Microscopic examination was performed after 2h to remove dead and injured individuals and to replenish 30 heads per slide.

2. Dissolving the raw materials with acetone, diluting with 0.1% Tween-80, respectively preparing single-dose mother liquor, setting multiple groups of ratios, and setting 6 gradient mass concentrations for each single dose and each group of ratio mixture according to an equal difference method;

3. soaking the glass slide in the liquid medicine, slightly shaking for 5S, taking out, sucking off the redundant liquid medicine by using absorbent paper, placing the liquid medicine in a white magnetic disk padded with wet sponge, and covering the white magnetic disk with a plastic film with good light transmittance. Each treatment was repeated 4 times and a treatment without agent was blanked.

4. Placing a container containing the treated test insects at the temperature of (25 +/-1) DEG C, wherein the photoperiod is L: and D ═ 16: 8) h. Investigating the death condition of the test insects after 48h, respectively recording the total number of the insects and the number of the dead insects of each treatment, calculating the corrected death rate of each treatment according to the total number of the insects and the number of the dead insects, performing regression analysis on the log values of the concentrations of each treatment medicament and the corrected death rate probability values of each treatment by using DPS software, and calculating LC of each treatment medicament₅₀And calculating the co-toxicity coefficient (CTC value) of the mixture according to the Sun Yunpei method.

P ═ K/N × 100, where P is mortality (%), K is dead number of worms, and N is total number of treated worms;

P₁＝(P_t－P₀)/(1－P₀) In which P is₁To correct for mortality (%), P_tTo address mortality (%), P₀Blank control mortality (%);

measured toxicity index (ATI) ═ standard medicament LC₅₀Test agent LC₅₀)×100；

Theoretical virulence index (TTI) ═ a agent virulence index × percentage of a in the mixture + B agent virulence index × percentage of B in the mixture;

co-toxicity coefficient (CTC) × 100 [ measured toxicity index (ATI) of the mixture)/Theoretical Toxicity Index (TTI) of the mixture ].

Criteria are divided according to joint action: the co-toxicity coefficient (CTC) is more than or equal to 120 and shows a synergistic effect; the co-toxicity coefficient (CTC) is less than or equal to 80, and the antagonism is shown; 80< co-toxicity coefficient (CTC) <120 showed additive effect. The results are shown in tables 1-3.

TABLE 1 indoor bioactivity assay of ethiazole mite nitrile and scoparone formulation on macadamia nut tea yellow mite

Name and proportion of the medicament	LC50(mg/L)	ATI	TTI	CTC
					Chlorobipyr	1.32	100.00	--	--
Scoparone	283.12	0.47	--	--
					Ethiazole mite nitrile 1: scoparone 40	28.61	4.61	2.89	159.43
Ethiazole mite nitrile 1: scoparone 30	15.18	8.70	3.68	236.49
					Ethiazole mite nitrile 1: scoparone 20	13.95	9.46	5.21	181.76
Ethiazole mite nitrile 1: scoparone 15	6.48	20.37	6.69	304.62
					Ethiazole mite nitrile 1: scoparone 10	1.74	75.86	9.51	797.31
Ethiazole mite nitrile 1: scoparone 5	4.72	27.97	17.06	163.97
					Ethiazole mite nitrile 1: scoparone 1	1.13	116.81	50.23	232.54
Ethiazole mite nitrile 5: scoparone 1	0.54	244.44	83.41	293.06
					Ethiazole mite nitrile 10: scoparone 1	0.41	321.95	90.95	353.98
Ethiazole mite nitrile 15: scoparone 1	0.66	20.00	93.78	213.27
					Ethiazole mite nitrile 20: scoparone 1	0.38	347.37	95.26	364.65
Ethiazole mite nitrile 30: scoparone 1	0.21	628.57	96.79	649.42
					Ethiazole mite nitrile 40: scoparone 1	0.78	169.3	97.57	173.44

As can be seen from Table 1, the mass ratio of the ethacrylonitrile to the scoparone is 1-40: the cotoxicity coefficients within the range of 40-1 are all larger than 120, namely the biological activity of the macadamia nut yellow tea mite is shown as a synergistic effect after the pyraclonil and the scoparone are compounded. Especially when the mass ratio is 1: when the dosage is 10, the co-toxicity coefficient reaches 797.31, and the synergistic effect is particularly obvious.

TABLE 2 indoor bioactivity assay of tebufenpyrad and scoparone formulation on macadamia nut yellow tea mites

As can be seen from Table 2, the mass ratio of the cyflumetofen to the scoparone is 1-15: the co-toxicity coefficients within the range of 60-1 are all larger than 120, namely the synergistic effect is shown on the biological activity of the macadamia nut yellow tea mites after the cyflumetofen and the scoparone are compounded. Especially when the mass ratio is 7: when 1, the co-toxicity coefficient reaches 846.44, and the synergistic effect is particularly obvious.

TABLE 3 indoor bioactivity assay of etoxazole and scoparone for compounded pair of macadamia nut yellow tea mites

As can be seen from Table 3, etoxazole and scoparone are mixed at a mass ratio of 1-20: the cotoxicity coefficients within the range of 5-1 are all larger than 120, namely the etoxazole and the scoparone are compounded to show a synergistic effect on the biological activity of the macadimia nut yellow tea mites.

In conclusion, when the pyraclonil, the cyflumetofen or the etoxazole and the scoparone are compounded in a certain mass ratio range, the co-toxicity coefficients are all higher than 120, the synergistic effect is obvious, the control effect on the macadimia nut yellow tea mites can be improved, and the development of novel chemical agents can be facilitated.

The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.