阅读说明：本技术 异噁唑类化合物在杀虫剂中的应用 (Application of isoxazole compound in pesticide ) 是由 开振鹏 凌烨 邵万策 于 2021-08-13 设计创作，主要内容包括：本发明涉及异噁唑类化合物在杀虫剂中的应用,其中,异噁唑类化合物具体为异噁唑类化合物FB23,或异噁唑类化合物FB23-2,其结构式分别如下所示：与现有技术相比,本发明拓宽了化合物FB23和FB23-2的应用领域,首次将能诱导害虫同类相食的化合物运用于害虫绿色防控中,用于防治多种农业害虫,尤其对草地贪夜蛾、斜纹夜蛾、小菜蛾及甜菜夜蛾具有显著的防治效果。(The invention relates to an application of an isoxazole compound in a pesticide, wherein the isoxazole compound is specifically an isoxazole compound FB23 or an isoxazole compound FB23-2, and the structural formulas of the isoxazole compound FB23 and the isoxazole compound FB23-2 are respectively shown as follows:)

1. The application of an isoxazole compound in pesticides is characterized in that the isoxazole compound has the following structural formula:

2. the application of the isoxazole compound in the pesticide as claimed in claim 1, wherein the isoxazole compound is used for preparing the pesticide for controlling Spodoptera frugiperda.

3. The use of an isoxazole compound as claimed in claim 1 in insecticides, wherein the isoxazole compound is used for preparing insecticides for controlling prodenia litura.

4. The use of the isoxazole compound in the pesticide according to claim 1, wherein the isoxazole compound is used for preparing the pesticide for controlling diamondback moth.

5. The application of the isoxazole compound in the pesticide as claimed in claim 1, wherein the isoxazole compound is used for preparing the pesticide for controlling beet armyworm.

6. The application of an isoxazole compound in pesticides is characterized in that the isoxazole compound has the following structural formula:

7. the application of the isoxazole compound in the pesticide as claimed in claim 6, wherein the isoxazole compound is used for preparing the pesticide for controlling Spodoptera frugiperda.

8. The application of the isoxazole compound in the pesticide as claimed in claim 6, wherein the isoxazole compound is used for preparing the pesticide for controlling prodenia litura.

9. The isoxazole compound is used as the pesticide according to claim 6, and is characterized by being used for preparing the pesticide for controlling diamondback moth.

10. The application of the isoxazole compound in the pesticide as claimed in claim 6, wherein the isoxazole compound is used for preparing the pesticide for controlling beet armyworm.

Technical Field

The invention belongs to the technical field of pesticides, and relates to application of isoxazole compounds in pesticides.

Background

Cannibalism with the same category is a phenomenon widely existing and distributed in nature. There are various manifestations of congeneric food: eating the same kind is the most direct behavior, which generally occurs when individuals with larger body size eat smaller body size; secondly, this behavior is manifested in eating similar unhatched eggs and larvae, which usually occur in multiple biological populations developing metamorphously, requiring rapid nutrient absorption to develop into adults; again, the mutual attack behavior between species in the same species, which occurs between species in which both parties compete for food or invade the same kind of nest, may result in both abortions and both parties attacking and dying. Cannibalism between species is an adaptive feature evolved due to food deficiency, and since many generations of individuals are exposed to a poorly nourished environment for a long time, the selected population evolves a more efficient way of hunting for food, which is adaptable, has sufficient genetic variation, and evolves in a short time. This may also explain that even under conditions of adequate food, species may be prone to cannibalism.

Cannibalism is documented in many orders of insects, including orthoptera, blattaria, hemiptera, coleoptera, hymenoptera, lepidoptera and diptera. Cannibalism is particularly common among lepidopteran insects. When the larvae reach a higher larval density, they will disperse and eventually assume a uniform spatial distribution. Studies have reported that plants decrease their food consumption by defending to induce increased self-killing behavior of insects. Therefore, a novel approach is provided for green prevention and control of pests by developing pesticides capable of inducing pests to eat like.

The compounds FB23 and FB23-2 are used in life science and medical research, but their use as pesticides has not been reported.

Disclosure of Invention

The invention aims to provide a novel and green pest control technology, namely an application of isoxazole compounds capable of inducing pests to eat like the same phase in pesticides.

The purpose of the invention can be realized by the following technical scheme:

an application of isoxazole compounds in pesticides is disclosed, wherein the isoxazole compounds are specifically isoxazole compounds FB23(CAS registration number: 2243736-35-6) or isoxazole compounds FB23-2(CAS registration number: 2243736-45-8), and the structural formulas of the isoxazole compounds are respectively shown as follows:

furthermore, the isoxazole compound can be used for preparing pesticides and can be applied to agricultural pest control.

Furthermore, the isoxazole compound can be used for preparing pesticides for controlling Spodoptera frugiperda.

Furthermore, the isoxazole compound can be used for preparing a pesticide for controlling prodenia litura.

Furthermore, the isoxazole compound can be used for preparing pesticides for controlling diamondback moths.

Furthermore, the isoxazole compound can be used for preparing pesticides for controlling beet armyworm.

The insecticidal mechanism is different from other commercial insecticides, and isoxazole compounds FB23 and FB23-2 can induce lepidoptera pest larvae to cannibalism with each other, reduce population density and achieve the aim of preventing and controlling the lepidoptera pests. Therefore, a novel approach for green prevention and control of pests is provided, and the insecticidal composition has very obvious effect on spodoptera frugiperda, prodenia litura, plutella xylostella and spodoptera exigua.

Compared with the prior art, the invention has the following characteristics:

1) the invention widens the application field of the compounds FB23 and FB23-2, and applies the compounds FB23 and FB23-2 to pesticides for the first time, in particular applies the compounds which can induce pests to eat like one another to green prevention and control of the pests for the first time;

2) compared with the pesticide mainly acting on a pest nervous system in the prior art, the isoxazole compounds FB23 and FB23-2 can induce pests to eat at the same class, so that the control of various agricultural pests can be realized, and the control effect on Spodoptera frugiperda, Spodoptera litura, plutella xylostella and Spodoptera exigua is particularly remarkable.

Detailed Description

The present invention will be described in detail with reference to specific examples. In the present embodiment, the technical solution of the present invention is used as a precondition for implementation, and a detailed implementation manner and a specific operation process are given, but the scope of the present invention is not limited to the following embodiments.

Compounds FB23 and FB23-2 in the following examples are available from Selleck Chemicals; the insects were purchased from Biopesticide Co., Ltd, Keyun, Henan province, and bred in the laboratory.

The test insect feeding conditions are as follows: the feed is purchased from biological pesticide of cloud of Henan province, and the water source is tap water, the temperature is 28 + -1 deg.C, the relative humidity is 50-70%, and the light cycle is L14: D10.

Example 1:

and (3) determining the influence of the compounds FB23 and FB23-2 on cannibalism behavior of pests.

1. The test method comprises the following steps:

the method reported in the reference of this example (Nat Ecol Evol 2017,1:1205-1207) measures the effects of compounds FB23 and FB23-2 on feeding behavior of Spodoptera frugiperda, Spodoptera litura, Plutella xylostella and Spodoptera exigua, and specifically comprises the following steps:

selecting a plurality of normal and healthy 4-instar larvae and normal and healthy 3-instar larvae, and respectively putting one 4-instar larva and one 3-instar larva into the goblet with enough food; the experimental group (set with 5 biological replicates) and the blank control group were set with 10 cups as one group. The compound was diluted to 10 with acetone^-5-10^-7M, separately dropped on the dorsal forethorax of 4-instar larvae in the experimental cups at a dose of 5. mu.L/body by the dropping method. The blank control was spotted with the same dose of acetone. Observing and recording whether cannibalism behaviors occur in the cup within 24 h.

2. Analysis and discussion of results:

according to observation of a 24-hour cannibalism experiment, the compounds FB23 and FB23-2 are found to have an enhancing effect on cannibalism behavior of Spodoptera frugiperda, Spodoptera litura, plutella xylostella and Spodoptera exigua. Tables 1 and 2 show the incidence of eating behavior of the same species of spodoptera frugiperda, spodoptera litura, plutella xylostella and spodoptera exigua treated with compounds FB23 and FB23-2 at different concentrations within 24 h.

TABLE 1 incidence of cannibalism behavior (%)% in 24h of Compound FB 23-treated pests

	Spodoptera frugiperda	Prodenia litura	Diamondback moth	Beet armyworm
					10-5M treatment	94±2	72±6	84±10	78±8
10-6M treatment	52±6	36±4	28±2	26±2
					10-7M treatment	12±2	8±4	0	0
Blank control	0	0	0	0

TABLE 2 incidence of cannibalism behavior in 24h of Compound FB23-2 treated pests

	Spodoptera frugiperda	Prodenia litura	Diamondback moth	Beet armyworm
					10-5M treatment	86±8	92±5	78±6	84±8
10-6M treatment	48±4	62±4	36±4	32±4
					10-7M treatment	8±2	10±4	12±2	6±2
Blank control	0	0	0	0

Experimental results show that the compounds FB23 and FB23-2 have certain promotion effects on cannibalism behavior of Spodoptera frugiperda, Spodoptera litura, Plutella xylostella and Spodoptera exigua. Larvae of 4 th instar who have cannibalism will eat all 3 rd instar within 24 hours. The group of 4-instar larvae still eat feed after cannibalism, which shows that the action mechanism of the drug does not make the larvae refuse to eat but induces the larvae to generate cannibalism.

In the field, pests with different pest ages coexist, and the compounds FB23 and FB23-2 can induce the pests to have cannibalism behavior, so that old pests can eat low-age pests, and the aims of reducing pest density and protecting crops are fulfilled.

The embodiments described above are described to facilitate an understanding and appreciation of the invention by those skilled in the art. It will be readily apparent to those skilled in the art that various modifications to these embodiments may be made, and the generic principles described herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present invention is not limited to the above embodiments, and those skilled in the art should make modifications and alterations without departing from the scope of the present invention.