阅读说明：本技术 一种含松油烯-4醇的生物制剂与生物杀虫剂混合使用方法 (Method for using biological preparation containing terpinen-4-ol and biological insecticide in mixing mode ) 是由 任启均 曹亭 杨廉勇 于 2021-10-25 设计创作，主要内容包括：本发明公开了一种含松油烯-4醇的生物复合制剂及其制备方法,包括松油烯-4醇0.01-100重量份、含氰基的邻苯二甲酰胺类化合物0.01-100重量份、十二烷基硫酸钠1-10重量份、木质素磺酸钠1-10重量份、硫酸铵1-10重量份和阿拉伯胶1-10重量份。本发明含松油烯-4醇的生物复合制剂具有增效的灭杀效果,特别适用于灭杀甘蓝小菜蛾。(The invention discloses a biological compound preparation containing terpinene-4 alcohol and a preparation method thereof, which comprises 0.01-100 parts by weight of terpinene-4 alcohol, 0.01-100 parts by weight of cyano-containing phthalic acid amide compounds, 1-10 parts by weight of sodium dodecyl sulfate, 1-10 parts by weight of sodium lignosulfonate, 1-10 parts by weight of ammonium sulfate and 1-10 parts by weight of Arabic gum. The biological compound preparation containing terpinen-4-ol has a synergistic killing effect, and is particularly suitable for killing cabbage plutella xylostella.)

1. A terpinen-4-ol containing biocomposite formulation comprising:

0.01-100 parts of terpinene-4-ol, 0.01-100 parts of cyano-containing phthalic diamide compounds, 1-10 parts of sodium dodecyl sulfate, 1-10 parts of sodium lignosulfonate, 1-10 parts of ammonium sulfate and 1-10 parts of Arabic gum.

2. The terpinen-4-ol-containing biocomposite preparation as claimed in claim 1, wherein the terpinen-4-ol is present in an amount of 0.1 to 10 parts by weight, the cyano-containing phthalic acid amide compound is present in an amount of 0.1 to 10 parts by weight, the sodium lauryl sulfate is present in an amount of 4 parts by weight, the sodium lignosulfonate is present in an amount of 2 parts by weight, the ammonium sulfate is present in an amount of 4 parts by weight, and the gum arabic is present in an amount of 2 parts by weight.

3. The bio-composite formulation containing terpinen-4-ol according to claim 1 or 2, wherein the terpinen-4-ol is prepared from cinnamomum camphora.

4. The terpinen-4-ol-containing biocomposite formulation as claimed in claims 1 to 3, wherein the phthalic acid diamide compound is cyhalodiamide.

5. A method for using the terpinen-4 alcohol-containing biocomposite preparation as claimed in any one of claims 1 to 4, wherein the method comprises spraying the terpinen-4 alcohol-containing biocomposite preparation as claimed in any one of claims 1 to 4 to plants to kill plant pests.

6. The method of using the terpinen-4 alcohol-containing biocomposite preparation of claim 4, wherein the plant is cabbage.

7. The use method of the terpinen-4-ol-containing biocomposite preparation as claimed in any one of claims 4 to 6, wherein the plant pest is diamond back moth.

Technical Field

The invention relates to the field of agricultural application, in particular to a biological compound preparation containing terpinene-4-ol and a using method thereof.

Background

Cabbage (Brassica oleracea L.): is annual or biennial herb of Brassicaceae and Brassica, and biennial herb, and is frosted. Short and strong annual stem flesh, no branching, green or grayish green. The basal leaves are thick and are wrapped layer by layer into spheroids, oblate spheres, milky white or light green; the biennial stem has branches with cauline leaves. The top end of the basal leaf is round, the basal part is narrowed to be a very short petiole with wide fins, and the edge is provided with wavy and unobvious sawteeth; the upper cauline leaves are oval or long round oval, and the base embraces the stem; the uppermost leaf is round, 4.5 cm long and 1 cm wide, and embraces the stem. The raceme grows from the top and the axilla; the flower is light yellow, and the diameter is 2-2.5 cm; the length of the flower stalk is 7-15 mm; the sepals are upright and are in a linear long circle shape; the petals are wide, elliptical, inverted oval or nearly circular, the top ends are slightly deficient, the base parts are narrowed into claws, and the length of the claws is 5-7 mm. The silique is cylindrical, the two sides are slightly flattened, the midrib is protruded, and the beak is conical; the fruit stalks are thick and spread vertically. Seeds are spherical, brown. The flowering period is 4 months, and the fruit period is 5 months.

In the cultivation process of the cabbages, the plutella xylostella is a major worldwide vegetable pest and mainly damages brassicaceous vegetables such as cabbages, cauliflowers, cabbages, green vegetables, rapes, radishes and the like. The generation number is large in the year, the damage time is long, each insect state of the plutella xylostella can be seen all the year round in Huaning county, the phenomenon of overwintering and overwintering is not obvious, the low-age larvae before 2 years old are drilled into the mesophyll to take food, only the epidermis of the leaf is left after the food is taken, and transparent spots are formed, so that the Chinese cabbage is called as 'skylight opening'. The 3-4 instar larvae damage leaves to form notches or holes, and become net-shaped when serious, thus seriously affecting the quality of vegetables. Vegetable growers are quite difficult to control, not only increasing the cost in the aspect of control, but also influencing the quality of vegetable products.

Cinnamomum camphora (with the academic name of Camphorum longipaniticulatum (Gamble) N. Chao ex H.W.Li) is evergreen arbor of Lauraceae, with height of 20 m and diameter at breast height of 50 cm; the bark is gray, the branch is cylindrical, hairless, the young branch is fine, the leaves are intergrowth, oval or elliptical, the tip is suddenly short and gradually sharp to long and gradually sharp, the upper surface is dark green, bright, the lower surface is grey green, dark, the two surfaces are hairless, the leaf stalk is flat and back convex, light green, the cone inflorescence axillary, is fine, the branch is fine and weak, each branch is 3-7 flowers of the polypodium, the shaft is hairless, the flower is light yellow, fragrance is provided, the stalk is fine, the flower quilt is cone-shaped, the flower quilt is oval, the filament is white and soft, the filament is glandless, the anther is long round, the ovary is oval, the young fruit is spherical, the flower is in 5-6 months, and the fruit is yielded in 7-9 months.

Yibin is the primary resource of the cinnamomum camphora, and by the end of 2018, 47 ten thousand mu of cinnamomum camphora forest base in the whole market has the crude cinnamomum camphora oil yield of over 70 percent of the whole country, the yibin crude cinnamomum camphora has high oil yield and good oil quality, and belongs to a non-inspection product in the international market.

At present, the main methods for extracting the crude camphor oil comprise a CO2 supercritical extraction method, a water vapor steam extraction method, an extraction method and the like, and target products are extracted from the crude camphor oil through the extraction methods, wherein terpinene-4 alcohol is one of the target products.

The existing terpinen-4 alcohol is generally used as an essential oil component, and other purposes are not reported.

Disclosure of Invention

In view of the above problems, in one aspect, the present invention provides a terpinen-4-ol-containing biocomposite preparation having a synergistic effect of killing cabbage plutella xylostella.

A terpinen-4-ol containing biocomposite formulation comprising:

0.01-100 parts of terpinene-4-ol, 0.01-100 parts of cyano-containing phthalic diamide compounds, 1-10 parts of sodium dodecyl sulfate, 1-10 parts of sodium lignosulfonate, 1-10 parts of ammonium sulfate and 1-10 parts of Arabic gum.

In one or more specific embodiments of the present application, the terpinen-4-ol is 0.1 to 10 parts by weight, the cyano-containing phthalic acid diamide compound is 0.1 to 10 parts by weight, the sodium dodecyl sulfate is 4 parts by weight, the sodium lignosulfonate is 2 parts by weight, the ammonium sulfate is 4 parts by weight, and the gum arabic is 2 parts by weight.

In one or more specific embodiments herein, the terpinen-4-ol is prepared from sassafras.

In one or more specific embodiments herein, the phthalic acid diamide compound is cyhalodiamide.

In one aspect, the invention also provides a method for using the biological compound preparation containing terpinen-4-ol.

The application method of the biological compound preparation containing terpinene-4 alcohol is characterized in that the biological compound preparation containing terpinene-4 alcohol is sprayed on plants to kill plant pests.

In one or more specific embodiments of the present application, the plant is brassica oleracea.

In one or more specific embodiments of the present application, the plant pest is diamondback moth.

The invention principle and the beneficial effects of the application are as follows:

the cyhalodiamide and the terpinen-4-ol are mixed to form the biological compound preparation, and surprisingly, the synergistic effect of killing the cabbage plutella xylostella is achieved.

Detailed Description

The present invention will be further explained below.

The examples provided herein are merely to further illustrate the invention and should not be construed as limiting the invention in any way.

It will be apparent to those skilled in the art that the materials and methods of operation used in the present invention are well known in the art, unless otherwise specified, in the following.

In the present invention, parts by weight means measured in units of weight, such as 1 part by weight means: when the weight is Kg, 1 part by weight means 1 Kg; when the weight is g, 1 part by weight means 1g, and the like, and is not limited to a specific Kg or g.

In the following examples, cyhalodiamide, terpinen-4-ol, sodium dodecyl sulfate, sodium lignosulfonate, ammonium sulfate are all commercially available, wherein terpinen-4-ol is prepared by extracting crude camphor oil.

Example 1

10 parts of cyhalodiamide, 0.1 part of terpinene-4 alcohol, 4 parts of sodium dodecyl sulfate, 2 parts of sodium lignosulfonate, 4 parts of ammonium sulfate and 2 parts of Arabic gum are uniformly mixed, and the mixture is added into water to prepare a composition solution 1 with 10ppm of cyhalodiamide and 0.1ppm of terpinene-4 alcohol as active ingredients for later use.

Example 2

Taking 10 parts by weight of cyhalodiamide, 4 parts by weight of sodium dodecyl sulfate, 2 parts by weight of sodium lignosulfonate, 4 parts by weight of ammonium sulfate and 2 parts by weight of Arabic gum, uniformly mixing, adding the mixture into water, and preparing a composition solution 2 with 10ppm of cyhalodiamide as an active ingredient for later use.

Example 3

Taking 0.1 part by weight of terpinene-4 alcohol, 4 parts by weight of sodium dodecyl sulfate, 2 parts by weight of sodium lignosulfonate, 4 parts by weight of ammonium sulfate and 2 parts by weight of Arabic gum, uniformly mixing, adding the mixture into water, and preparing a composition solution 3 with 0.1ppm of terpinene-4 alcohol as an active ingredient for later use.

Example 4

Taking 10 parts by weight of cyhalodiamide, 1 part by weight of terpinene-4 alcohol, 4 parts by weight of sodium dodecyl sulfate, 2 parts by weight of sodium lignosulfonate, 4 parts by weight of ammonium sulfate and 2 parts by weight of Arabic gum, uniformly mixing, adding the mixture into water, and preparing a composition solution 4 with 10ppm of cyhalodiamide and 1ppm of terpinene-4 alcohol as active ingredients for later use.

Example 5

Taking 10 parts by weight of cyhalodiamide, 4 parts by weight of sodium dodecyl sulfate, 2 parts by weight of sodium lignosulfonate, 4 parts by weight of ammonium sulfate and 2 parts by weight of Arabic gum, uniformly mixing, adding the mixture into water, and preparing a composition solution 5 with 10ppm of cyhalodiamide as an active ingredient for later use.

Example 6

Taking 1 part by weight of terpinene-4 alcohol, 4 parts by weight of sodium dodecyl sulfate, 2 parts by weight of sodium lignosulfonate, 4 parts by weight of ammonium sulfate and 2 parts by weight of Arabic gum, uniformly mixing, adding the mixture into water, and preparing a composition solution 6 with 1ppm of terpinene-4 alcohol as an active ingredient for later use.

Example 7

Taking 6 parts by weight of cyhalodiamide, 5 parts by weight of terpinene-4 alcohol, 4 parts by weight of sodium dodecyl sulfate, 2 parts by weight of sodium lignosulfonate, 4 parts by weight of ammonium sulfate and 2 parts by weight of Arabic gum, uniformly mixing, adding the mixture into water, and preparing a composition solution 7 with 10ppm of cyhalodiamide and 5ppm of terpinene-4 alcohol as active ingredients for later use.

Example 8

Taking 6 parts by weight of cyhalodiamide, 4 parts by weight of sodium dodecyl sulfate, 2 parts by weight of sodium lignosulfonate, 4 parts by weight of ammonium sulfate and 2 parts by weight of Arabic gum, uniformly mixing, adding the mixture into water, and preparing a composition solution 8 with 10ppm of cyhalodiamide as an active ingredient for later use.

Example 9

5 parts by weight of terpinene-4 alcohol, 4 parts by weight of sodium dodecyl sulfate, 2 parts by weight of sodium lignosulfonate, 4 parts by weight of ammonium sulfate and 2 parts by weight of Arabic gum are taken and uniformly mixed, and the mixture is added into water to prepare a composition solution 9 with 5ppm of terpinene-4 alcohol as an active ingredient for later use.

Example 10

The method comprises the following steps of uniformly mixing 1 part by weight of cyhalodiamide, 10 parts by weight of terpinene-4 alcohol, 4 parts by weight of sodium dodecyl sulfate, 2 parts by weight of sodium lignosulfonate, 4 parts by weight of ammonium sulfate and 2 parts by weight of Arabic gum, and adding the mixture into water to prepare a composition solution 10 with 1ppm of cyhalodiamide and 10ppm of terpinene-4 alcohol as active ingredients for later use.

Example 11

Taking 1 part by weight of cyhalodiamide, 4 parts by weight of sodium dodecyl sulfate, 2 parts by weight of sodium lignosulfonate, 4 parts by weight of ammonium sulfate and 2 parts by weight of Arabic gum, uniformly mixing, adding the mixture into water, and preparing a composition solution 11 with 1ppm of cyhalodiamide as an active ingredient for later use.

Example 12

Taking 10 parts by weight of terpinene-4 alcohol, 4 parts by weight of sodium dodecyl sulfate, 2 parts by weight of sodium lignosulfonate, 4 parts by weight of ammonium sulfate and 2 parts by weight of Arabic gum, uniformly mixing, adding the mixture into water, and preparing a composition solution 12 with 10ppm of terpinene-4 alcohol as an active ingredient for later use.

Example 13

Taking 0.1 part by weight of cyhalodiamide, 10 parts by weight of terpinene-4 alcohol, 4 parts by weight of sodium dodecyl sulfate, 2 parts by weight of sodium lignosulfonate, 4 parts by weight of ammonium sulfate and 2 parts by weight of Arabic gum, uniformly mixing, and adding the mixture into water to prepare a composition solution 13 with 0.1ppm of cyhalodiamide and 10ppm of terpinene-4 alcohol serving as active ingredients for later use.

Example 14

Taking 0.1 part by weight of cyhalodiamide, 4 parts by weight of sodium dodecyl sulfate, 2 parts by weight of sodium lignosulfonate, 4 parts by weight of ammonium sulfate and 2 parts by weight of Arabic gum, uniformly mixing, adding the mixture into water, and preparing a composition solution 14 with 0.1ppm of cyhalodiamide as an active ingredient for later use.

Example 15

Taking 10 parts by weight of terpinene-4 alcohol, 4 parts by weight of sodium dodecyl sulfate, 2 parts by weight of sodium lignosulfonate, 4 parts by weight of ammonium sulfate and 2 parts by weight of Arabic gum, uniformly mixing, adding the mixture into water, and preparing a composition solution 15 with 10ppm of terpinene-4 alcohol as an active ingredient for later use.

Example 16

Selecting healthy cabbage without insect pest, inoculating 50 larvae of diamondback moth to each cabbage, and spraying composition solution 1-15 and pure water (as blank control). Wherein, each composition solution is a treatment, each treatment corresponds to 10 cabbages, and the spraying amount of each treatment is consistent. After 7 days of each treatment, the mortality was investigated and finally calculated (3 replicates were taken as an average), and the results are shown in table 1 below, where in table 1 the theoretical insecticidal rate is the insecticidal rate calculated using the Colby formula.

TABLE 1

As can be seen from Table 1, the blending of the cyhalodiamide with terpinen-4-ol as the active ingredients has a synergistic effect of killing cabbage moths.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.