阅读说明：本技术 一种纤溶激活物添加剂 (Fibrinolytic activator additive ) 是由 张善财 任江杰 于 2021-06-29 设计创作，主要内容包括：本发明公开了一种纤溶激活物添加剂及其制备方法,主要包括下述原料和步骤：a.按蝇蛆30%,黄粉虫10%,蚯蚓10%,蜗牛20%,动物内脏类30%的重量百分数配比选择原料,分离掉其它杂质,捣碎得到浆液；b.浓浆液加入磁处理水,重量百分比为：浓浆液30%,重磁水70%,放入高速搅拌机中搅拌,在不沉降状态下,分离杂质,得到清浆；c.清浆在孵育罐中保持37℃恒温孵育,孵育48小时至96小时,经过多层滤膜器过滤后,进入离心机,在3000rpm离心机里离心15min得到纯浆,取出纯浆放入－10℃的冷柜内预冷3小时；d.取出解冻,加入乙醇,调含醇量为体积比80％的乙醇,体积配比为：纯浆99%,乙醇1%,进入搅拌机拌匀,进入干燥机干燥为粉状,即得。(The invention discloses a fibrinolysis activator additive and a preparation method thereof, which mainly comprises the following raw materials and steps: a. selecting raw materials according to the weight percentage of 30 percent of fly maggots, 10 percent of yellow mealworms, 10 percent of earthworms, 20 percent of snails and 30 percent of animal viscera, separating other impurities, and mashing to obtain slurry; b. adding magnetic treatment water into the concentrated slurry, wherein the weight percentage is as follows: 30% of the concentrated slurry and 70% of the heavy magnetic water are put into a high-speed stirrer to be stirred, and impurities are separated under the condition of no sedimentation, so that clear slurry is obtained; c. incubating the clear pulp in an incubation tank at a constant temperature of 37 ℃, incubating for 48 to 96 hours, filtering by a multi-layer filter membrane, entering a centrifuge, centrifuging for 15min in the centrifuge with 3000rpm to obtain pure pulp, taking out the pure pulp, and placing the pure pulp in a refrigerator with the temperature of-10 ℃ for precooling for 3 hours; d. taking out, unfreezing, adding ethanol, adjusting the ethanol content to 80% of ethanol by volume ratio, and mixing the volume ratio: 99% of pure pulp and 1% of ethanol are put into a stirrer to be uniformly stirred and then are put into a dryer to be dried into powder, thus obtaining the product.)

1. A fibrinolysis activator additive mainly comprises the following raw materials in percentage by weight: 30% of fly maggots, 10% of yellow mealworms, 10% of earthworms, 20% of snails and 30% of animal viscera.

2. A preparation method of a fibrinolysis activator additive mainly comprises the following steps:

a. selecting raw materials according to the weight percentage of 30 percent of fly maggots, 10 percent of yellow mealworms, 10 percent of earthworms, 20 percent of snails and 30 percent of animal viscera, separating the raw materials in a screening tank to remove other impurities, and mashing the raw materials to obtain slurry;

b. adding magnetic treatment water into the concentrated slurry, wherein the weight percentage is as follows: 30% of the concentrated slurry and 70% of the heavy magnetic water are put into a high-speed stirrer to be stirred, and impurities are separated under the condition of no sedimentation, so that clear slurry is obtained;

c. incubating the clear pulp in an incubation tank at a constant temperature of 37 ℃, incubating for 48 to 96 hours, filtering the incubated clear pulp by a multilayer filter membrane, putting the filtered clear pulp into a centrifuge, centrifuging for 15min in the centrifuge with 3000rpm to obtain pure pulp, taking out the pure pulp, and putting the pure pulp into a refrigerator with the temperature of-10 ℃ for precooling for 3 hours;

d. taking out the pure pulp, unfreezing, adding ethanol, adjusting the ethanol content to 80% of ethanol by volume ratio, and mixing the volume ratio: mixing 99% pure pulp, 1% ethanol and ethanol-containing pulp in a stirrer, and drying in a dryer to obtain powder product.

Technical Field

The invention relates to a fibrinolytic activator additive, belonging to the technical field of biotechnology breeding.

Background

The selection of the feed is an important link of the livestock breeding industry, and most of the feed used in the livestock breeding at present is a mixture of animal meat and bone meal, soybean meal, corn, wheat and the like. Insufficient supply of feed raw materials has gradually become an important problem in the feed production process. The ratio of the weight gain of the livestock to the feed amount in the breeding process is a key point related to the breeding cost and the benefit, and the feed is fed for a certain amount of time, so that the larger the weight gain, particularly the muscle gain of the livestock is, the lower the cost is. In order to improve the proportion, there are not only several approaches, one is to explore different feed types and proportions, but the method is researched by a plurality of breeding scientific researchers, and basically has no space to be improved; secondly, the livestock with higher muscle content and faster growth gene is introduced or cultivated, but a huge amount of capital is consumed, and the cost is hard to bear by common breeding enterprises; finally, various additives are added into the feed to improve the absorption ratio of the livestock to the feed. However, the added additive must be safe and sanitary and meet the requirements of relevant standards, so the research on the aspect has certain development prospect and significance.

Disclosure of Invention

The invention aims to solve the technical problem of providing a fibrinolytic activator additive which is added into livestock feed to improve the content of fiber protein, promote the absorption and digestion of livestock, improve the feed conversion rate and activate specific immune response in intestinal tracts of livestock; in addition, the invention also provides a preparation method of the fibrinolysis activator additive.

The technical scheme of the invention is as follows: a fibrinolysis activator additive mainly comprises the following raw materials in percentage by weight: 30% of fly maggots, 10% of yellow mealworms, 10% of earthworms, 20% of snails and 30% of animal viscera.

A preparation method of a fibrinolysis activator additive mainly comprises the following steps:

a. selecting raw materials according to the weight percentage of 30 percent of fly maggots, 10 percent of yellow mealworms, 10 percent of earthworms, 20 percent of snails and 30 percent of animal viscera, separating the raw materials in a screening tank to remove other impurities, and mashing the raw materials to obtain slurry;

b. adding magnetic treatment water into the concentrated slurry, wherein the weight percentage is as follows: 30% of the concentrated slurry and 70% of the magnetic treated water are put into a high-speed stirrer to be stirred, and impurities are separated under the condition of no sedimentation, so that clear slurry is obtained;

c. incubating the clear pulp in an incubation tank at a constant temperature of 37 ℃, incubating for 48 to 96 hours, filtering the incubated clear pulp by a multilayer filter membrane, putting the filtered clear pulp into a centrifuge, centrifuging for 15min in the centrifuge with 3000rpm to obtain pure pulp, taking out the pure pulp, and putting the pure pulp into a refrigerator with the temperature of-10 ℃ for precooling for 3 hours;

d. taking out the pure pulp, unfreezing, adding ethanol, adjusting the ethanol content to 80% of ethanol by volume ratio, and mixing the volume ratio: mixing 99% pure pulp, 1% ethanol and ethanol-containing pulp in a stirrer, and drying in a dryer to obtain powder product.

The invention has the beneficial effects that:

1. the fibrinolytic activator additive prepared by the raw materials and the method has high activity: the activity of exo-beta-1, 4-glucanase reaches 37IU/mg, and the activity of endo-beta-1, 4-glucanase reaches 41 IU/mg; the activity of endo-beta-1, 4-xylanase is as high as 196 IU/mg.

2. The fibrinolysis activator additive prepared by the invention is added into feed, can crack crystalline cellulose, and acts on the surface of insoluble fiber to crack crystalline fiber chains and free the end parts of long-chain cellulose molecules, so that the cellulose chains are easy to hydrate.

3. The fibrinolysis activator additive prepared by the invention is added into feed, can decompose fiber and pectin in mash, fully release starch and protein, promote vigorous fermentation of yeast and increase productivity; reduce the viscosity of mash, accelerate the liquefaction and saccharification of raw materials, shorten the filtration time and reduce the power consumption.

The invention is added into livestock feed, can activate intestinal specific immune reaction, improve the conversion rate of vegetable protein in fiber-containing feed, degrade ammonia nitrogen and nitroso ammonia, decompose hydrogen sulfide and improve the absorption rate of ammonia.

Detailed Description

Example one

A preparation method of a fibrinolysis activator additive mainly comprises the following steps:

a. selecting 100kg of raw materials according to the weight of 30kg of fly maggots, 10kg of yellow mealworms, 10kg of earthworms, 20kg of snails and 30kg of animal viscera, separating the raw materials in a screening tank to remove other impurities, and mashing the raw materials to obtain slurry;

b. adding 150kg of magnetic treatment water into the concentrated slurry, putting the concentrated slurry into a high-speed stirrer for stirring, and separating impurities under the condition of no settlement to obtain 200kg of clear slurry;

c. incubating the clear slurry in an incubation tank at a constant temperature of 37 ℃, incubating for 48 hours, filtering the incubated clear slurry by a multilayer filter membrane, putting the filtered clear slurry into a centrifuge, centrifuging for 15min in the centrifuge with 3000rpm to obtain 140kg of pure slurry, taking out the pure slurry, and putting the pure slurry into a refrigerator with the temperature of-10 ℃ for precooling for 3 hours;

d. thawing the pure pulp, adjusting ethanol content to 80% by volume, adding 1.4L ethanol, stirring the ethanol-containing pulp in a stirrer, and drying in a dryer to obtain powder product.

Example two

A preparation method of a fibrinolysis activator additive mainly comprises the following steps:

a. selecting 100kg of raw materials according to the weight of 30kg of fly maggots, 10kg of yellow mealworms, 10kg of earthworms, 20kg of snails and 30kg of animal viscera, separating the raw materials in a screening tank to remove other impurities, and mashing the raw materials to obtain slurry;

b. adding 150kg of magnetic treatment water into the concentrated slurry, putting the concentrated slurry into a high-speed stirrer for stirring, and separating impurities under the condition of no settlement to obtain 200kg of clear slurry;

c. incubating the clear slurry in an incubation tank at a constant temperature of 37 ℃, incubating for 96 hours, filtering the incubated clear slurry by a multi-layer filter membrane, putting the filtered clear slurry into a centrifuge, and centrifuging for 15min in the centrifuge at 3000rpm to obtain 170kgPure pulp is taken out and put into a freezer with the temperature of minus 10 ℃ for precooling for 3 hours;

d. thawing the pure pulp, adjusting ethanol content to 80 vol% ethanol, adding 1.7L ethanol, stirring the ethanol-containing pulp in a stirrer, and drying in a dryer to obtain powder product.

In the first embodiment of the invention, 0.5% of the traditional feed is added, the traditional feed added with the invention, the imported feed and the traditional feed small fish are selected as samples, and the comprehensive benefit comparison of the grouper cultured in the 100 cubic meter net cage is carried out:

the comparison shows that when the traditional feed is added to breed the groupers in the net cage of 100 cubic meters, the comprehensive benefit is 87.5 percent higher than that of the groupers using the feed, and the benefit is 20 percent higher than that of the groupers using the imported feed;

in the second embodiment of the invention, 0.5 percent of the traditional feed is added, and the traditional feed, the imported puffed feed, the non-cured sinking material and the self-prepared feed are selected to be added for carrying out the comprehensive benefit comparison of each mu of prawn culture:

when the traditional feed added with the invention is used for culturing prawns, the comprehensive income per mu is increased by 50 percent compared with that of non-cured submerged materials, is increased by 28.5 percent compared with that of imported puffed materials, is increased by 3 times compared with self-batching materials, the feeding period is shortened, and the feeding frequency per year reaches 2-3 times which is higher than that of other three feeds.