阅读说明：本技术 一种提高储存期鸡蛋相对密度的方法及应用 (Method for improving relative density of eggs in storage period and application ) 是由 李留安 毛帅强 秦顺义 于晓雪 于亚萍 孙英峰 张建斌 于 2021-09-23 设计创作，主要内容包括：本发明公开了一种提高储存期鸡蛋相对密度的方法及应用。该利用蛋鸡体内对叶酸和茶多酚的代谢和转化作用,将特定的饲料添加剂(25-40：60-75的叶酸和茶多酚)以0.4%-1.25%的添加量添加于蛋鸡饲料中,饲喂蛋鸡20-30天,能够提高储存期鸡蛋的相对密度,进而提升鸡蛋的经济价值。实验结果显示：在存储第20(相对密度为1.07)和30天(相对密度为1.073)时,试验组鸡蛋的相对密度均极显著高于对照组。提高了储存期鸡蛋的相对密度,进而达到延长鸡蛋保质期的目的。(The invention discloses a method for improving the relative density of eggs in a storage period and application. The feed additive (25-40: 60-75 folic acid and tea polyphenol) is added into the feed of the laying hens in an amount of 0.4-1.25% by utilizing the metabolism and conversion of folic acid and tea polyphenol in the laying hens, and the relative density of eggs in the storage period can be improved by feeding the laying hens for 20-30 days, so that the economic value of the eggs is improved. The experimental results show that: the relative density of the eggs in the test group was significantly higher than that in the control group at both 20 (relative density of 1.07) and 30 days (relative density of 1.073) of storage. The relative density of the eggs in the storage period is improved, and the purpose of prolonging the shelf life of the eggs is further achieved.)

1. A method for improving the relative density of eggs in the storage period is characterized in that specific feed additives are added into layer feed, and the specific feed additives comprise the following components in parts by weight of 25-40: 60-75 percent of folic acid and tea polyphenol, and the addition amount is 0.4-1.25 percent of the weight of the common feed.

2. The method for increasing the relative density of eggs on storage according to claim 1, wherein the laying hens are fed with a general feed supplemented with 0.4% to 1.25% of a specific feed additive for 20 to 30 days.

3. Use of the method of claim 1 for increasing the relative density of eggs during storage.

Technical Field

The invention belongs to the field of livestock and poultry product production in animal husbandry, and particularly relates to a method for improving the relative density of eggs in a storage period.

Background

Eggs are one of the best nutrition sources, each hundred grams of eggs contain 12.8 g of protein, and the digestibility of the protein can reach 98 percent; each hundred grams of eggs contain 11-15 g of fat, and are easily digested and absorbed by the organism; the egg is rich in lecithin, sterols, egg yolk, calcium, phosphorus, iron, vitamin A, vitamin D and vitamin B complex. Therefore, the eggs are an ideal nutrition bank. Under the condition of ordinary storage, the relative density of eggs is gradually reduced along with the prolonging of storage time, the relative density of fresh eggs is between 1.08 and 1.09, and the relative density of old eggs is between 1.02 and 1.06; the freshness of the eggs can be identified by measuring the relative density of the eggs, and the relative density of the eggs has a positive correlation with the quality and the shelf life of the eggs. Therefore, the relative density of the eggs in the storage period is improved, the purpose of prolonging the quality guarantee period of the eggs is further achieved, and the production practice is particularly urgent; the method has important application value and production practice significance for improving the economic benefit of laying hen breeding and promoting the healthy development of poultry industry.

The invention achieves the purpose of improving the relative density of eggs in the storage period by using the metabolism and the conversion of folic acid and tea polyphenol in the bodies of laying hens through a method for regulating and controlling the nutrition of the feed. Until now, no similar patent or research report is found.

Disclosure of Invention

The invention aims to provide a method for improving the relative density of eggs in storage period; the method can prolong the shelf life of eggs, and is helpful for improving the economic benefit of laying hen breeding.

In order to achieve the purpose, the invention discloses the following technical scheme:

a method for improving the relative density of eggs in the storage period is characterized in that specific feed additives are added into layer feed, and the specific feed additives comprise the following components in parts by weight of 25-40: 60-75% folic acid and tea polyphenols; the addition amount of the specific feed additive is 0.4-1.25% of the weight of the common feed. The method comprises the following steps: a specific feed additive is added into a common feed for laying hens. As a limitation: the addition amount of the specific feed additive in the common feed for the laying hens is 0.2-1.25% of the weight of the feed. As a further limitation: the feeding scheme is that the laying hens are fed with feed containing 0.2-1.25% of specific feed additives for 20-30 days, and the laying hens are fed with free food and water, and are subjected to conventional management and immunization. The invention proposes that the feeding management method of the laying hens and the preparation of the feed can be determined according to related national regulations and feeding standards.

The invention further discloses application of the method in improving the relative density of eggs in a storage period, and experimental results show that: the invention can effectively improve the relative density of eggs in the storage period, and the relative density of the eggs in the test group is remarkably higher than that in the control group when the eggs are stored for 20 days (the relative density is 1.07) and 30 days (the relative density is 1.073). The relative density of the eggs in the storage period is improved, and the purpose of prolonging the shelf life of the eggs is further achieved.

The method for improving the relative density of the eggs in the storage period disclosed by the invention has the beneficial effects that:

the aim of improving the relative density of eggs in the storage period is fulfilled by utilizing the metabolism and conversion effects of the laying hens on folic acid and tea polyphenol, so that the aim of prolonging the quality guarantee period of the eggs is fulfilled, and the economic value of the eggs is remarkably improved.

The invention mainly solves the problem of improving the relative density of eggs in the storage period. The method mainly aims to examine the influence of the screening of the effective addition amount of folic acid and tea polyphenol in the feed and the feeding time on the relative density of eggs in the storage period, and has the main difficulty that the screening of the effective addition amount of folic acid and the determination of the feeding time are determined, which is the most important creative research content in the invention, so that the following tests and researches are carried out:

firstly, determining the effective dosage range of folic acid in the laying hen feed. The influence of folic acid with different concentrations of 10, 20, 40, 80, 160, 320, 600, 1200, 1800g/kg and the like added into the feed on the relative density of eggs in the storage period is examined; as a result, it was found that when the amount of folic acid added to the feed for layer hens was less than 80g/kg, no effect of increasing the relative density of eggs during storage was exhibited (see Table 1); when the additive amount of the folic acid in the laying hen feed exceeds 600g/kg, the folic acid does not have the effect of improving the relative density of eggs in the storage period, so that the effective dose range of the folic acid in the laying hen feed is finally determined to be 80-600 g/kg.

TABLE 1 influence of feed with different concentrations of Folic acid on relative density of eggs during storage

Note: "+" indicates significant difference from the control group ((C))P< 0.05 >, "-" indicates no significant difference from the control group: (P>0.05）。

Regarding the determination of feeding time, we examined the effect of folic acid at concentrations of 10, 20, 40, 80, 160, 320, 600, 1200, 1800g/kg on the relative density of eggs during storage for 10, 20, 30 days respectively; the feeding time was determined to be 20-30 days (see table 1).

Secondly, the ratio of the three components in the specific additive is determined. The mixture ratio of the two components is screened according to the table 2, and the mixture ratio is 25-40% (w/w) of folic acid and 60-75% (w/w) of tea polyphenol.

TABLE 2 screening of the proportions of the ingredients in the additives

Finally, the specific feed additive for improving the relative density of the eggs in the storage period is determined to be composed of 25-40 parts by weight: 60-75% folic acid and tea polyphenols.

Finally, the amount of specific additives added to the feed is determined. The dosage of the additive is determined to be 0.4-1.25% according to the effective dosage range of folic acid in the laying hen feed and the comprehensive consideration of cost factors.

Detailed Description

The invention is described below by means of specific embodiments. Unless otherwise specified, the technical means used in the present invention are well known to those skilled in the art. In addition, the embodiments should be considered illustrative, and not restrictive, of the scope of the invention, which is defined solely by the claims. It will be apparent to those skilled in the art that various changes or modifications in the components and amounts of the materials used in these embodiments can be made without departing from the spirit and scope of the invention. The raw materials and reagents used in the present invention are commercially available, and for example, folic acid, tea polyphenol and the like are commercially available.

Examples 1 to 6

Method for improving relative density of eggs in storage period

Examples 1 to 6 are methods for increasing the relative density of eggs in the storage period, which are methods for feeding laying hens by adding a specific feed additive to a feed for laying hens; except for the composition ratios of the specific feed additives and the additive amounts of the additives in each example (table 3).

Table 3 examples 1-6 compositions and amounts of specific feed additives in feed

Example 7

Verification test of method for improving storage period egg relative density

Certain free-range laying hen farm in Tianjin City

(1) Practice of animals and groups

Selecting healthy northern China firewood chicken laying hens of 35 weeks old as 90 feathers, and randomly dividing the feathers into a control group and a test group, wherein each group has 3 repetitions, and each repetition has 15 feathers.

(2) Daily ration and feeding management

Feeding basic ration for the control group, and matching the nutrition required by the laying hens according to NRC and the chicken feeding standard in China; the test group added 1.25% of a specific feed additive (the specific feed additive comprises folic acid and tea polyphenol in a weight ratio of 25: 75) to the basic ration; all groups of laying hens are kept in cages indoors, and free eating and drinking water are adopted in the test process, and conventional immunization is carried out; after the group is pre-tested for 1 week by feeding basic ration, feeding corresponding test ration for 20 days.

(3) Sample collection and index determination

At 20 days of the experiment, 20 eggs were randomly selected from each group for testing the relative density of eggs during storage. And respectively detecting the relative density of the eggs after being stored for 20 and 30 days at normal temperature.

(4) Test effects

The test results are shown in Table 4, and show that the relative density of the eggs in the test group is remarkably higher than that in the control group on 20 th and 30 th days of storage (P﹤0.01）。

TABLE 41.25 Effect of specific feed additives on the relative Density of eggs on storage

Note: means significant difference from the control group: (P< 0.05) < showing extremely significant difference from control group >P﹤0.01）。

And (4) conclusion: the invention can effectively improve the relative density of eggs in the storage period, ensure the freshness of the eggs and further achieve the purpose of prolonging the shelf life of the eggs.