阅读说明：本技术 一种犬用营养粉及其制备方法和应用 (Nutritional powder for dogs and preparation method and application thereof ) 是由 吴怡 彭晓培 胡金华 马东立 张泽宇 马曦 郑美大 夏黎明 于 2020-06-15 设计创作，主要内容包括：本发明提供了一种犬用营养粉及其制备方法和应用。具体地说,所述犬用营养粉包含如下组分或由如下组分组成：蛋黄粉、鸡肝粉、奶粉、啤酒酵母粉、葡萄糖、麦芽糊精、益生菌乳酸钙、磷酸氢钙、维生素C、维生素D3、酪蛋白磷酸肽和牛胶原蛋白。本发明还提供了一种犬用营养粉的制备方法以及其在犬饲喂中的应用。本发明能够显著促进犬的身体生长发育,强壮骨骼,增加体重,使犬的被毛看更顺滑、更有光泽。(The invention provides a nutritional powder for dogs and a preparation method and application thereof. Specifically, the nutritional powder for dogs comprises or consists of the following components: yolk powder, chicken liver powder, milk powder, beer yeast powder, glucose, maltodextrin, probiotic calcium lactate, calcium hydrophosphate, vitamin C, vitamin D3, casein phosphopeptide and bovine collagen. The invention also provides a preparation method of the nutritional powder for dogs and application of the nutritional powder in dog feeding. The invention can obviously promote the growth and development of the body of the dog, strengthen the skeleton, increase the weight and ensure that the fur of the dog looks smoother and glossier.)

1. A nutritional powder for dogs, wherein the nutritional powder comprises or consists of the following components: yolk powder, chicken liver powder, milk powder, beer yeast powder, glucose, maltodextrin, probiotic calcium lactate, calcium hydrophosphate, vitamin C, vitamin D3, casein phosphopeptide and bovine collagen.

2. The nutritional powder for dogs according to claim 1, wherein:

the nutritional powder for dogs comprises 1 to 3 parts by weight of egg yolk powder, 10 to 15 parts by weight of chicken liver powder, 2 to 5 parts by weight of milk powder, 5 to 8 parts by weight of beer yeast powder, 11 to 15 parts by weight of glucose, 11 to 15 parts by weight of maltodextrin, 10 to 14 parts by weight of probiotic fermented calcium lactate, 20 to 24 parts by weight of calcium hydrophosphate, 0.001 to 0.01 part by weight of vitamin D3, 0.5 to 1 part by weight of casein phosphopeptide and 1 to 2 parts by weight of bovine collagen.

3. The nutritional powder of claim 1 or 2, wherein:

the preparation method of the probiotic fermented calcium lactate comprises the following steps:

inoculating the activated lactobacillus suspension into a fermentation culture medium added with calcium carbonate for culture; and

extraction of calcium lactate from the culture: filtering the fermentation liquor to remove thalli and unreacted calcium carbonate, adding calcium oxide into the filtrate to adjust the pH value to 12-13, and adding MgSO₄Precipitating and polymerizing mycoprotein, filtering and crystallizing to obtain the probiotic fermented lactic acid.

4. The nutritional powder of any one of claims 1 to 3, wherein:

the content of the probiotic calcium lactate is preferably 11 to 13 parts by weight.

5. The nutritional powder of any one of claims 1 to 4, wherein:

the content of calcium hydrogen phosphate is preferably 21 to 23 parts by weight.

6. The nutritional powder of any one of claims 1 to 5, wherein:

the content of the vitamin D3 is preferably 0.005 to 0.008 parts by weight.

7. The nutritional powder of any one of claims 1 to 6, wherein:

the content of the casein phosphopeptide is preferably 0.6 to 0.8 parts by weight.

8. The nutritional powder of any one of claims 1 to 7, wherein:

the ratio of the probiotic calcium lactate to the calcium hydrogen phosphate is 1: 2-1: 1.88.

9. use of a nutritional powder for dogs to feed or prepare a feed for dogs, wherein:

the canine nutrition powder is the canine nutrition powder of any one of claims 1 to 8.

10. A method of making the canine nutritional powder of any one of claims 1 to 8, wherein the method comprises the steps of:

1) premixing: sieving probiotic calcium lactate, calcium hydrogen phosphate, milk powder, glucose, casein phosphopeptide, bovine collagen, chicken liver powder, yolk powder and maltodextrin with 100 mesh sieve to prevent agglomeration materials and impurities from mixing, and sieving and mixing the materials;

2) drying: putting the uniformly mixed materials into an oven for drying, and cooling the dried materials to room temperature;

3) total mixing: adding the vitamin C, the vitamin D3 and the beer yeast powder with the formula ratio into the cooled material, fully and uniformly mixing in a mixer, and sieving the uniformly mixed material by a 100-mesh sieve;

4) sterilizing: sterilizing before filling;

5) filling: transferring the sterilized powder to a filling line, and filling and sealing under a disinfection condition;

6) optional post-filling sterilization: and (4) sterilizing by adopting a pasteurization method after filling.

Technical Field

The invention relates to the technical field of pet food preparations, in particular to nutritional powder for dogs and a preparation method and application thereof.

Background

With the improvement of living standard and the development of society, more and more families are raising pets, especially pet dogs. According to the American Pet Products Association (APPA), nearly three quarters of American domestic pets are willing to spend a large amount of time and money for the young partners of the hairy antler to be happy. The pet industry has grown 2 times over the past 15 years and pet food has rapidly become a popular culture. How to raise the pet is a general concern for pet raising families.

Calcium supplementation for pets is nowadays receiving increasing attention. Calcium has important physiological functions for animals from young to adult and even old age, and is an essential element for ensuring the physical health of pets. Specifically, the aged pets have problems such as osteoporosis and bone deformation due to a decrease in calcium absorption ability and a large amount of calcium loss in the body caused by deterioration of physiological functions, and thus need to be supplemented with calcium on a daily basis. In addition, the calcium deficiency of pets may cause anorexia, listlessness, and anorexia, and may also cause digestive disorders such as diarrhea and constipation. Osteoporosis, susceptibility to fright, joint stiffness, pain, and bone deformation may occur in the middle of the period.

The juvenile period is the rapid growth period of pets, and once the calcium is insufficiently supplemented, the problems of slow bone development, bone deformation, unhealthy teeth and the like can be caused, so the calcium is necessarily supplemented.

Calcium supplementation can help tooth health: if the pet has the problems of tooth loss, tooth looseness and the like, proper calcium supplement can be selected to help strengthen the tooth health.

Restoration of nutrition in lactating bitches or queens: in the lactation period of the female dog or the female cat, a large amount of nutrient flows out, so that nutrition supplement, including calcium supplement, needs to be done to help the body and energy of the female dog or the female cat to recover as soon as possible.

Inorganic calcium (ionized calcium) is the earliest formulation, such as calcium carbonate, calcium hydrogen phosphate, calcium oxide, etc., which are present in high amounts, but they have one of the biggest drawbacks of requiring a large amount of gastric acid to participate. Before the calcium in the calcium preparation is absorbed, a large amount of gastric acid is needed to decompose the calcium and then the calcium can be absorbed by the body, but the large amount of gastric acid can stimulate the gastrointestinal tract. However, the absorption rate of the body to the calcium agent is only 5-20%, and the excessive calcium is excreted to the outside of the body through the kidney, and the risk of calculus is increased. The organic calcium does not need much gastric acid, has higher absorption rate and has much less irritation to the stomach.

Chinese patent application publication CN109984268A relates to a vitamin nutrient solution for calcium supplement for pets, which takes castor oil as solubilizer to prepare calcium and vitamin into liquid supplement.

Chinese patent application publication CN109497316A relates to a pet food for calcium supplement for dogs, which is prepared from lactobacillus acidophilus, calcium bicarbonate, vitamin D3, magnesium sulfate and corn starch.

Studies have shown that effective calcium supplementation focuses on more effective absorption, and none of the prior art provides calcium supplement formulations or foods that can enhance calcium absorption in dogs. In particular, the prior art has not found a calcium to phosphorus ratio that optimizes calcium absorption by pet dogs.

Disclosure of Invention

The inventor finds that different calcium sources, proper calcium-phosphorus ratios and proper calcium-phosphorus absorption promoting components can enhance the calcium absorption of dogs. Based on the findings, the invention provides, through experiments, in a first aspect, a nutritional powder for dogs, wherein the nutritional powder comprises egg yolk powder, chicken liver powder, milk powder, beer yeast powder, glucose, maltodextrin, probiotic calcium lactate, calcium hydrophosphate, vitamin C, vitamin D3, casein phosphopeptide and bovine collagen.

In a second aspect, the invention provides the use of a nutritional powder for dogs to feed puppies or to prepare a feed for dogs.

The present invention also provides in a third aspect a process for the preparation of a nutritional powder according to the first aspect of the invention, the process comprising the steps of:

1) premixing: sieving probiotic calcium lactate, calcium hydrogen phosphate, milk powder, glucose, casein phosphopeptide, bovine collagen, chicken liver powder, yolk powder and maltodextrin with 100-mesh sieve to prevent caking materials and impurities from being mixed, and uniformly mixing the materials after sieving:

2) drying: and (3) putting the uniformly mixed materials into an oven for drying, and cooling the dried materials to room temperature:

3) total mixing: adding the vitamin C, the vitamin D3 and the beer yeast powder with the formula ratio into the cooled material, fully and uniformly mixing in a mixer, and sieving the uniformly mixed material by a 100-mesh sieve:

4) sterilizing: sterilizing before filling:

5) filling: transferring the sterilized powder to a filling line, and filling and sealing under the sterilization condition:

6) optional post-filling sterilization: and (4) sterilizing by adopting a pasteurization method after filling.

Compared with the prior art, the invention has the following technical advantages: can obviously promote the growth and development of the body of the dog, strengthen the skeleton, increase the weight and ensure that the fur of the dog looks smoother and glossier.

Drawings

Fig. 1 is a graph showing the effect of the inventive nutritional powder for dogs and the control group on the coat condition of test dogs on days 2 and 5.

Fig. 2 is a graph showing the effect of the inventive nutrition powder for dogs and the blank and control groups on the coat state of test dogs on days 9 and 13.

Fig. 3 is a graph showing the effect of the inventive nutritional powder for dogs and the control group on the coat condition of test dogs on day 19.

Detailed Description

In order to solve one or more of the above technical problems in the prior art, the present invention provides, in a first aspect, a nutritional powder for dogs, wherein the nutritional powder comprises or consists of: yolk powder, chicken liver powder, milk powder, beer yeast powder, glucose, maltodextrin, probiotic calcium lactate, calcium hydrophosphate, vitamin C, vitamin D3, casein phosphopeptide and bovine collagen.

The yolk powder adopted by the invention is prepared by taking fresh eggs as raw materials through dozens of working procedures of cleaning, disinfection, egg beating, filtration, cooling, pasteurization, spray drying and the like, retains all nutrients of the yolk, contains amino acid, calcium, iron, phosphorus, vitamins, lecithin, taurine, lutein, zeaxanthin, DHA, EPA and the like required by animal organisms, and has the same nutrition with fresh yolk. The vitamin D belongs to fat-soluble vitamins, the absorption process of the vitamin D needs the participation of lipid, and the yolk powder provides a high-quality fat source for the effective absorption of the vitamin D.

The yolk powder content of the nutritional powder for dogs is 1 weight part to 3 weight parts, preferably 1.2 weight parts to 2.5 weight parts, and more preferably 1.5 weight parts to 2 weight parts.

The chicken liver powder adopted by the invention can be purchased commercially.

The content of the chicken liver powder in the nutritional powder for dogs is 10 to 15 parts by weight, preferably 11 to 14 parts by weight, and more preferably 12 to 13 parts by weight.

The milk powder adopted by the invention can be purchased commercially.

The content of the milk powder in the nutrition powder for dogs is 2 to 5 parts by weight, preferably 3 to 4 parts by weight, and more preferably 3.5 parts by weight.

The beer yeast powder used in the present invention is commercially available.

The content of the beer yeast powder in the dog nutrition powder is 5 to 8 parts by weight, preferably 6 to 7 parts by weight, and more preferably 6.5 parts by weight.

The glucose used in the present invention is commercially available.

The content of glucose in the nutritional powder for dogs is 11 to 15 parts by weight, preferably 12 to 14 parts by weight, and more preferably 13 parts by weight.

The maltodextrins useful in the present invention are commercially available.

The content of maltodextrin in the dog nutrition powder is 11 to 15 weight parts, preferably 12 to 14 weight parts, and more preferably 13 weight parts

The probiotic calcium lactate adopted by the invention has good water solubility and higher absorptivity than inorganic calcium, and simultaneously relieves the problem of canine constipation caused by inorganic calcium.

The content of the probiotic calcium lactate in the nutrition powder for dogs is 10 to 13 parts by weight, preferably 11 to 12 parts by weight, and more preferably 11.6 parts by weight.

The calcium lactate adopted by the invention is preferably probiotic fermented calcium lactate, and the probiotic fermented calcium lactate can retain a large amount of lactic acid bacteria metabolites (lactobacillin, polysaccharide, oligosaccharide, vitamin and the like), promote intestinal development and maintain intestinal health. In addition, the probiotic fermented calcium lactate has sour, fragrant and sweet smell, and has a good food calling effect on pets. Although the mechanism is not clear, the research of the invention finds that the calcium lactate composition produced by fermentation of probiotics has better growth promotion effect than calcium lactate from other sources. And the calcium lactate composition produced by fermenting with probiotics in a specific ratio is more suitable for promoting bone growth than other contents.

The preparation method of the probiotic fermented calcium lactate comprises the following steps:

1. activation of lactic acid bacteria: taking a ring of well-grown slant seeds, inoculating the seeds into a liquid activation culture medium, culturing in an incubator at 36 ℃, and continuously activating for 2 times for later use;

2. enrichment culture of lactic acid bacteria: under the aseptic condition, sucking a certain amount of activated bacterial suspension by a pipette, adding the bacterial suspension into an MRS culture medium, and culturing in a constant-temperature incubator;

3. acid production culture by lactic acid bacteria fermentation: adding 50mL of fermentation medium into a 250mL conical flask, sucking a certain amount of activated bacterial suspension by a pipette under the aseptic condition, adding the bacterial suspension into the fermentation medium, and placing the mixture into a constant-temperature incubator for culture;

4. preparing calcium lactate by a fermentation method: inoculating the activated bacterial suspension into a fermentation medium added with calcium carbonate, and culturing under the optimal condition obtained by the test;

5. extraction of calcium lactate: filtering the fermentation liquor obtained under the optimal condition to remove thalli and unreacted calcium carbonate, adding calcium oxide into the filtrate to adjust the pH value to 12-13, adding 0.4% MgSO4 to precipitate mycoprotein, stirring and reacting at 80 ℃ for 30min, carrying out suction filtration while the solution is hot to obtain a mother liquor of calcium lactate, heating and concentrating the mother liquor, and cooling, standing and crystallizing for 24 hours when the mass concentration of the calcium lactate in the mother liquor reaches 145-150 g/L; separating crystal and mother liquor, and concentrating, recrystallizing, and mixing crystals, wherein a large amount of calcium lactate is remained in the mother liquor due to the high solubility of calcium lactate in water.

The calcium hydrogen phosphate employed in the present invention is commercially available.

The content of the calcium hydrophosphate in the canine nutrition powder is 20 to 24 parts by weight, preferably 21 to 23 parts by weight, and more preferably 22 parts by weight.

The vitamin D3 adopted by the invention determines whether the organism has a strong skeletal development system or not, and promotes skeletal development.

The content of vitamin D3 in the nutritional powder for dogs is 0.001 to 0.01 weight part, preferably 0.005 to 0.008 weight part, and more preferably 0.006 weight part.

The content of casein phosphopeptide in the dog nutrition powder is 0.5 to 1 weight part, preferably 0.6 to 0.8 weight part, and more preferably 0.7 weight part.

The casein phosphopeptide adopted by the invention can generate synergistic effect when being used with calcium lactate, and can promote bone development.

Bovine collagen for use in the present invention is commercially available.

The content of the bovine collagen in the nutritional powder for dogs is 1 to 2 parts by weight, preferably 1.5 parts by weight.

The probiotics calcium lactate and calcium hydrogen phosphate provide calcium and phosphorus, and the ratio of the probiotics calcium lactate to the probiotics calcium hydrogen phosphate is 1: 2-1: 1.88, preferably 1.2: 1. if the ratio is outside, the calcium supplement effect on the dog may be reduced.

The ratio of the probiotic calcium lactate to the casein phosphopeptides in the invention is (10-14): (0.5-1), most preferably 12.75: 1. If the ratio is outside, no synergistic effect is produced, and it is difficult to maximally promote bone development.

The preparation method of the nutritional powder for dogs comprises the following steps:

1) premixing: sieving probiotic calcium lactate, calcium hydrogen phosphate, milk powder, glucose, casein phosphopeptide, bovine collagen, chicken liver powder, yolk powder and maltodextrin with 100-mesh sieve to prevent caking materials and impurities from being mixed, and uniformly mixing the materials after sieving:

2) drying: and (3) putting the uniformly mixed materials into an oven for drying, and cooling the dried materials to room temperature:

3) total mixing: adding the vitamin C, the vitamin D3 and the beer yeast powder with the formula ratio into the cooled material, fully and uniformly mixing in a mixer, and sieving the uniformly mixed material by a 100-mesh sieve:

4) sterilizing: sterilizing before filling:

5) filling: transferring the sterilized powder to a filling line, and filling and sealing under the sterilization condition:

6) optional post-filling sterilization: and (4) sterilizing by adopting a pasteurization method after filling.

Examples

The present invention will be further illustrated by the following examples, but the scope of the invention as claimed is not limited to these examples. The starting materials used in the examples are, unless otherwise indicated, conventional starting materials which are commercially available.

Preparation example

The following formula was used in the following table 1, 2kg of each nutritional powder was prepared:

TABLE 1 formulation of different nutritional powders

Nutritional powders A, B, C, D and E were prepared according to the above formulations A, B, C, D and E, respectively, the preparation method comprising the steps of:

1. premixing: sieving probiotic calcium lactate, calcium hydrogen phosphate, milk powder, glucose, casein phosphopeptide, bovine collagen, chicken liver powder, yolk powder and maltodextrin with 100 mesh sieve to prevent agglomeration materials and impurities from mixing, and sieving and mixing the materials;

2. drying: putting the uniformly mixed materials into an oven for drying at the drying temperature of 70 ℃ for 6 hours, and cooling the dried materials to room temperature;

3. total mixing: adding the vitamin C, the vitamin D3 and the beer yeast powder with the formula ratio into the cooled material, fully and uniformly mixing in a mixer, and sieving the uniformly mixed material by a 100-mesh sieve;

4. and (3) sterilization: sterilizing before filling;

5. filling: transferring the sterilized powder to a filling line, and respectively filling and sealing under the condition of opening a 2000-air-volume dynamic air sterilizer for 60 minutes (to avoid secondary pollution);

6. and (3) sterilizing after filling: and (4) after filling, sterilizing by adopting a pasteurization method (at 70-80 ℃ for 30-40 minutes).

The preparation method of the probiotic fermented calcium lactate comprises the following steps:

1. activation of lactic acid bacteria: taking a ring of well-grown slant seeds, inoculating the seeds into a liquid activation culture medium, culturing in an incubator at 36 ℃, and continuously activating for 2 times for later use;

2. enrichment culture of lactic acid bacteria: under the aseptic condition, sucking a certain amount of activated bacterial suspension by a pipette, adding the bacterial suspension into an MRS culture medium, and culturing in a constant-temperature incubator;

3. acid production culture by lactic acid bacteria fermentation: adding 50mL of fermentation medium into a 250mL conical flask, sucking a certain amount of activated bacterial suspension by a pipette under the aseptic condition, adding the bacterial suspension into the fermentation medium, and placing the mixture into a constant-temperature incubator for culture;

4. preparing calcium lactate by a fermentation method: inoculating the activated bacterial suspension into a fermentation medium added with calcium carbonate, and culturing under the optimal condition obtained by the test;

5. extraction of calcium lactate: filtering the fermentation liquor obtained under the optimal condition to remove thalli and unreacted calcium carbonate, adding calcium oxide into the filtrate to adjust the pH value to 12-13, adding 0.4% MgSO4 to precipitate mycoprotein, stirring and reacting at 80 ℃ for 30min, carrying out suction filtration while the solution is hot to obtain a mother liquor of calcium lactate, heating and concentrating the mother liquor, and cooling, standing and crystallizing for 24 hours when the mass concentration of the calcium lactate in the mother liquor reaches 145-150 g/L; separating crystal and mother liquor, and concentrating, recrystallizing, and mixing crystals, wherein a large amount of calcium lactate is remained in the mother liquor due to the high solubility of calcium lactate in water.

Test example 1

1 animal rearing

In the test, 15 beagle dogs (8 male dogs and 7 female dogs) are selected, all the beagle dogs are young dogs with the age of 8-10 months, and the initial weight difference is within 1 kg.

The test dogs are raised in a single cage, and are quantitatively fed with 300g of feed every day. The feed is fed for 1 time respectively in the morning and afternoon, and the water is freely drunk. Exercise time is available every day. Feces are cleared every day.

2. Design of experiments

According to the principle that the weight and sex ratio are similar, the test is divided into 3 groups, 4 blank control groups, 7 formula A groups and 4 formula B groups (control groups). The blank group was fed basic full-value dog food, formula a group was fed basic full-value dog food + formula a nutritional powder (2.8 g per time, 2 times per day), and formula B group (control group) was fed basic full-value dog food + formula B nutritional powder (2.8 g per time, 2 times per day), with the test beginning at 24 days 4 and 14 days 5 and continuing for 21 days in 2020.

See table 2 below.

TABLE 2 grouping of the tests

Note: 1. the addition amount of calcium is calculated according to the daily feed intake of 300g of young dogs.

3 sampling index and method

3.1 weight: body weights were weighed on days 0, 7, 14, 21 at the start of the test.

3.2 feed intake: feed intake was recorded 1 total time per dog per day,

3.3 case of pile: the condition of the hair of each dog was recorded 1 time by photographing every morning round, and the photograph required that the fullness and glossiness of the hair could be clearly seen.

3.4 skin condition: the skin state of each dog is recorded 1 time every morning round, and the back, chest and face of the dog are pulled out each time to observe whether the skin has erythema, erythra, dandruff and other abnormal conditions. Recorded in the table.

3.5 defecation cases: feces were recorded for each dog 1 time daily in the morning at the time of feces clearance and are recorded in the table.

3.6 mental states: the mental state of each dog was recorded 2 times each day during morning and evening rounds and scored.

3.7 selection of records: each dog was pulled daily in the morning on the induction diet 3 days prior to the trial and the first selected nutrition was recorded. After the traction test was completed, the above-mentioned routine recording was performed.

4. Results and discussion

4.1 body weight

The weights of the test dogs were recorded 4 times during the test period and the results are shown in table 3. The body weight changes on days 7, 14 and 21 of the test compared to before the start of the test (D0) are shown in table 4, and the body weight changes on each week of the test are shown in table 4. The body weight analysis conditions of the test dogs are shown in tables 5, 6 and 7.

Table 3 test dog body weight recording units: kg of

Description of the drawings: d0: the day before the start of the test; d7: day 7 of start of experiment; d14: day 14 after the start of the test, and so on.

Table 4 test dog body weight change units: kg of

Description of the drawings: d0: the day before the start of the test; d7: day 7 of start of experiment; d14: day 14 after the start of the test, and so on.

Table 5 mean body weight units of test dogs at different test stages: kg of

Group of	D0	D7	D14	D21
					Blank group	8.31	8.48	8.53	8.95
Formula A group	8.05	8.21	8.34	8.89
					Formulation BGroup of	9.03	9.15	9.25	9.55

Note: the data in the same column with different shoulder marks and lower case letters shows obvious difference; the data in the same row are marked with different capital letters, which indicates that the difference is extremely obvious; the difference is not significant if there is no or the same letter. The same applies below.

Table 6 average weight gain in units of test dogs at different test stages compared to initial body weight: kg of

Group of	D7-D0	D14-D0	D21-D0
				Blank group	0.16	0.21	0.64ab
Formula A group	0.16	0.29	0.84b
				Formulation group B	0.13	0.23	0.53a

Table 7 average weekly weight gain units for test dogs: kg of

Group of	D7-D0	D14-D7	D21-14
				Blank group	0.16	0.05A	0.43ab
Formula A group	0.16	0.13B	0.55b
				Formulation group B	0.13	0.10AB	0.30a

Statistical analysis is carried out on the data by using R language, and the result shows that the mean value difference of each group in the table 5 is not obvious; however, in table 6, there was a significant difference in the total weight gain over the entire trial period between formula a and formula B (control) (P0.0563); in table 7, the weight gain in the second week blank group was significantly lower than the formula a group (P ═ 0.0117), the weight gain in the third week formula B group (control) was significantly lower than the formula a group (P ═ 0.05),

as can be seen from table 5, the body weights of the dogs in each group did not differ significantly at each experimental stage. However, at the beginning of the test, the weight of formula B (control) was the greatest, weighing 0.98kg compared to formula A dogs, and at the end of the test, the difference was reduced to 0.66 kg. The blank weighed 0.26kg at the start of the test compared to formula a, and at the end of the test, the gap narrowed to 0.06 kg. These data indicate that the rate of weight gain during the trial was greater for formula a over the blank and formula B. The data in tables 6 and 7 also demonstrate that there is a significant difference between the groups from the point of view of weight gain. Table 6 shows that the total weight gain (sum of the weight gains over the 3-week trial period) was the most with nutritional powder type a and was significantly different from nutritional powder type B. Table 7 shows that at week 2 of the experiment, the week weight gain of the group of type a nutritional powders was the highest, and the difference from the blank group was very significant; at week 3 of the trial, the weekly weight gain of the nutritional powder group type a was still the greatest and was significantly different from that of the nutritional powder group type B.

From the body weight data, the type a nutritional powder performed best and the type B nutritional powder was the worst. The A-type nutrition powder is more suitable for calcium-phosphorus ratio, so that the A-type nutrition powder is considered to promote the utilization of minerals such as calcium, phosphorus and the like in dog food, and the weight of the dog is increased most quickly.

4.2 selection of records

The results of the nutrient selection test are shown in table 8. Statistical analysis is carried out on the data by using the R language, and the result shows that the palatability of different nutritional powders has obvious difference, and the palatability of the B type nutritional powder is obviously reduced (P ═ 0.04975). In table 8, the diets added with the type a nutritional powder were selected the most frequently, the basal diets were the worst, and the diets added with the type B nutritional powder were the worst.

TABLE 8 test dog selection daily ration

Daily ration	Basal dog food	Basic dog food + A type nutrition powder	Basic dog food + B type nutrition powder
				Number of selections	15ab	21a	10b

Note: the data in the same row with different shoulder marks and lower case letters shows obvious difference; those with the same letters indicate that the difference is not significant.

Based on this result, another type of selection test was performed next. We have bought the "Weishi" brand calcium tablet and "Kai Rui" brand calcium tablet from the market. One piece of the two calcium tablets and 2.0 g of the A-type nutrition powder and the B-type nutrition powder used in the test are respectively taken and placed in front of dogs to be eaten, only one dog in 15 dogs eats four kinds of calcium, but the other 14 dogs eat the nutrition powder. This demonstrates that the nutritional powder of the present invention is more palatable than tablets.

4.3 case of pile

See table 9. Except for slight depilation of individual dogs, abnormal conditions of other quilts were not observed. The blank group had a mild alopecia in 1 dog on day 5 of the test, in the formula a group on day 19 of the test, and in the formula B group (control group) in each of 1 dog on days 5 and 17. This is sufficient to indicate that the nutritional powder of the present invention is not harmful to beagle dogs.

From the images of the furred hairs (fig. 1, fig. 2, fig. 3), the formula a and formula B groups (control group) appeared smoother and glossier than the furred hairs of the placebo dogs. Of these, formula group a performed best. This indicates that calcium and phosphorus are also beneficial to the hair than a suitable nutritional powder.

TABLE 9 abnormal statistics of the piloerection of test dogs

Note: the coat status of one dog was scored as 1 dog per day.

4.4 skin Condition

From the recorded table, the skin was free of dandruff, red rash, red swelling and other abnormalities, except that individual dogs exhibited mild alopecia. Indicating that the nutritional powder of the present invention is not harmful to dogs.

4.5 fecal Condition

The fecal status of each group of test dogs is summarized in table 10. The abnormal condition of the feces of each group has no significant difference through the analysis of R language (P ═ 1). Throughout the test period, 1 dog in each of the blank groups showed brown hard stools on days 5, 16 and 19, 1 dog in each of the formula A groups showed brown hard stools on days 4, 11 and 17-18, and 1 dog in each of the formula B groups (control group) showed brown hard stools on days 4-7, 11 and 16.

In general, the nutritional powder has certain influence on the excrement of dogs, and is easy to cause hard stool, especially in a B type nutritional powder group with improper calcium and phosphorus ratios. But not to the health of the dog.

TABLE 10 statistics of abnormal stool status of test dogs

Note: fecal status of one dog a day was scored as 1 dog.

4.6 mental states

From the records, no mental abnormalities appeared in the dogs in each group. This indicates that the nutritional powder of the present invention is not harmful to beagle dogs. In the test process, all the dogs are active, namely active activities such as barking, walking, tail rocking, climbing and the like are available except blinking.

4.7 ingestion

From the record table, some dogs feed less than 300g per day. Over the entire experimental period, the blank group was fed to each dog with mean food intake of 297.9g per day, formula a was 298.6g, formula B (control) was 299.0g, and the data were analyzed by variance analysis in R language, which indicated that there was no significant difference in mean food intake for each group (P ═ 0.097). This indicates that the nutritional powder of the present invention is not harmful to beagle dogs.

5. Conclusion

The nutrition powder is harmless to dogs from the viewpoints of fur, skin, excrement, mental conditions and feed intake, and can be eaten for a long time.

From the point of weight, the growth promoting effect of the A-type nutritional powder is obvious. From the viewpoint of nutritional powder composition, the type a nutritional powder should be more beneficial to bones.

From the results of the selective experiments, the nutritional powder of the invention has good palatability. From the point of being fed, the palatability of the nutrition powder is higher than that of a full-value dog food (21 times vs.15 times).

Test example 2

1. Animal feeding

8 beagle dogs (4 male and 4 female) are young dogs with the age of 8-10 months, and the initial weight difference is within 1 kg. The tarsal joint of the left hind limb of each dog was artificially designed to be inflamed and swollen.

The test dogs are raised in a single cage, and are quantitatively fed with 300g of feed every day. The feed is fed for 1 time respectively in the morning and afternoon, and the water is freely drunk. Exercise time is available every day. Feces are cleared every day.

2. Design of experiments

According to the principle that the weight and sex proportion are similar, the test is divided into 4 groups, 1 male dog and 1 female dog in each group have 2 dogs, and different nutrition powder is added into the daily ration of each group of dogs respectively. Group a was fed basic full-rate dog food + formula a nutritional powder (2.8 g per day, 2 times per day), group C was fed basic full-rate dog food + formula C nutritional powder (2.8 g per day, 2 times per day), group D was fed basic full-rate dog food + formula D nutritional powder (2.8 g per day, 2 times per day), and group E was fed basic full-rate dog food + formula E nutritional powder (2.8 g per day, 2 times per day).

The test started on day 5, 15 and ended on day 5, 6, 2020 for 21 days.

See table below.

TABLE 11 test grouping

Note: 1. the test dog calculated the amount of calcium added according to the daily feed intake of 300 g.

3. Sampling index and method

Tarsal joint circumference: the circumference of the tarsal joint of the left hind limb of the dog was measured with a tape measure on days 0, 7, 14, and 21, respectively, from the start of the test.

4. Results and discussion

The circumference of the tarsal joint of the test dog was recorded 4 times during the test period and the results are shown in table 12.

Table 12 test canine tarsal joint circumference record units: mm is

Description of the drawings: d0: the day before the start of the test; d7: day 7 of start of experiment; d14: day 14 after the start of the test, and so on.

As can be seen from Table 12, there was a reduction in the degree of tarsal joint swelling in the left hind limb of each group of dogs, with the best recovery in group A dogs, the mean reduction in the swollen joint circumference over the entire trial period of 14.5mm, which far exceeded 8mm, 8.5mm, 10mm in the other groups. This indicates that formula a nutritional powder is more beneficial for bone growth.

From the composition of each group of nutrition powder, the addition amount of the probiotic fermented calcium lactate in the A-type nutrition powder is 12.5%, the C-type nutrition powder only uses 12.5% of common calcium lactate, and the addition amounts of the probiotic calcium lactate in the D-type nutrition powder and the E-type nutrition powder are 9% and 15% respectively. Among the test dogs, the reduction in the swollen tarsal joints of group C was minimal over the 21 day test period, indicating that the absorption and utilization of probiotic fermented calcium lactate by animals was superior to that of non-fermented calcium lactate. The reduction of tarsal joints of dogs in group D and E is less than that of dogs in group A in a 21-day test period, which indicates that the higher the contents of calcium and phosphorus in the daily ration, the more beneficial the bone marrow health of animals is, the calcium-phosphorus ratio in the daily ration and the proper dosage of probiotic fermented calcium lactate must be considered.

5. Conclusion

From the recovery condition of the tarsal joints of the swollen left hind limb of the beagle dog, the formula A nutrition powder is more beneficial to the bone health of the dog, which shows that the formula A is more scientific in proportion and more in line with the nutritional requirements of the dog.

Test example 2 fully demonstrates that suitable levels of probiotic calcium lactate have a better effect on bone health in dogs than ordinary calcium lactate, or unsuitable levels of probiotic calcium lactate.

Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit of the corresponding technical solutions of the embodiments of the present invention.