阅读说明：本技术 一种含硝虫硫磷的杀虫组合物 (Insecticide composition containing nitre sulfur and phosphorus ) 是由 郑敬敏 冯建雄 刘欢 于 2021-09-16 设计创作，主要内容包括：本发明属于杀虫剂领域,具体涉及一种含有硝虫硫磷的杀虫组合物。有效活性成分包括硝虫硫磷和吡丙醚,硝虫硫磷和吡丙醚的质量比是20:1-1：10。该组合物在实际应用中可制成农药上允许的多种剂型,比如乳油、水乳剂、微乳剂、可湿性粉剂、水分散粒剂、可分散液剂、微囊悬浮剂等。该组合物对农作物害虫具有一定的增效作用,包括白粉虱、蓟马、介壳虫、木虱、潜叶蛾、草地贪夜蛾、玉米螟、粘虫、斜纹夜蛾、甜菜夜蛾、小菜蛾等,可以有效延缓害虫抗性,减少农药使用量,保护环境。(The invention belongs to the field of pesticides, and particularly relates to a pesticide composition containing nitrobenfuron. The effective active ingredients comprise the components of the nitenpyram and the pyriproxyfen, and the mass ratio of the nitenpyram to the pyriproxyfen is 20:1-1: 10. the composition can be prepared into various formulations allowed by pesticides in practical application, such as missible oil, aqueous emulsion, microemulsion, wettable powder, water dispersible granules, dispersible liquid, microcapsule suspending agent and the like. The composition has a certain synergistic effect on crop pests, comprises whitefly, thrips, scale insects, psyllids, leaf miners, spodoptera frugiperda, ostrinia nubilalis, armyworms, prodenia litura, asparagus caterpillar, plutella xylostella and the like, can effectively delay the resistance of the pests, reduce the using amount of pesticides and protect the environment.)

1. An insecticidal composition containing nitrofos, which is characterized in that: the effective active ingredients comprise the components of the nitenpyram and the pyriproxyfen, and the mass ratio of the nitenpyram to the pyriproxyfen is 20:1-1: 10.

2. the insecticidal composition containing nitrofos according to claim 1, characterised in that: the mass ratio of the nitrofos to the pyriproxyfen is 20:1-1: 8.

3. the insecticidal composition containing nitrofos according to claim 1, characterised in that: the mass ratio of the nitrofos to the pyriproxyfen is 6:1-3: 1.

4. the insecticidal composition containing nitrofos according to claim 1, characterised in that: the total weight percentage of the nifedipine and the pyriproxyfen in the composition is 1 to 90 percent.

5. The insecticidal composition containing nitrofos according to claim 1, characterised in that: the formulation of the composition is missible oil, aqueous emulsion, microemulsion, wettable powder, water dispersible granules, dispersible liquid and microcapsule suspending agent.

6. Use of the insecticidal composition containing nitrofos according to any one of claims 1-4 for controlling agricultural pests.

7. The use according to claim 5, wherein the agricultural pests are whitefly, thrips, scale insects, psyllids, leaf miners, spodoptera frugiperda, ostrinia nubilalis, armyworm, prodenia litura, spodoptera exigua, plutella xylostella.

Technical Field

The invention relates to an insecticidal composition containing nitre sulfur and phosphorus, belonging to the technical field of pesticide compounding.

Background

The nifedipine is an organophosphorus insecticide and acaricide independently developed by chemical research and design institute of Sichuan province, has both stomach toxicity and contact poisoning activity, and has better permeability in plant bodies. It belongs to an acetylcholine ester inhibitor in insect bodies and causes death of pests by blocking nerve conduction of pests. At present, the nitenpyram is registered on the citrus arrowhead scale for use, and researches show that the nitenpyram has outstanding control effects on pests such as citrus red spider, arrowhead scale, cotton bollworm, vegetable oriental tobacco budworm, diamond back moth, rice planthopper, rice thrips and the like.

Pyriproxyfen, also known as pyriproxyfen, has the action mechanism of inhibiting the activity of insect allay and interfering the biosynthesis of ecdysone, and can be used for dry control of various pests such as homoptera, thysanoptera, diptera, lepidoptera and the like. Especially has good ovicidal action to various pests with piercing-sucking and rasping-sucking mouthparts, has inhibiting effect on pupation and eclosion of mosquito and fly larvae, and is also a good sanitary pesticide. It has the characteristics of high efficiency, small dosage, long lasting period, safety to crops, low toxicity to fish, small influence on ecological environment and the like.

The pest resistance problem is always a key problem in the process of plant chemical protection, the pest resistance problem is increasingly prominent due to the influence of factors such as increase of the usage amount of pesticides and unscientific use of pesticides, and the main method for solving the resistance problem is to use pesticide blending to delay the pest resistance at present. In addition, the composition is used together to enhance the effect, so that the dosage per unit area can be reduced, the cost is reduced, and the environment is protected.

Disclosure of Invention

The invention aims to provide an insecticidal composition with synergistic effect, which comprises the following components:

(1) a first active ingredient: nitenpyram;

(2) the second active ingredient: pyriproxyfen

The invention also aims to provide application of the composition in preventing and controlling pests and mites on crops.

In order to achieve the purpose, the technical scheme of the invention is as follows:

the insecticidal composition containing pyriproxyfen has the weight ratio of the first active ingredient to the second active ingredient of 20:1-1:10, preferably 6:1-3: 1. In the insecticidal composition, the total weight of the effective components accounts for 1-90% of the weight of the pesticide composition, and the balance is an auxiliary agent.

The pyriproxyfen-containing insecticidal composition can be prepared into different formulations suitable for agricultural production according to a method known by a person skilled in the art, and the preferable formulations include missible oil, aqueous emulsion, microemulsion, wettable powder, water dispersible granules, dispersible liquid, microcapsule suspending agent and the like.

For emulsifiable concentrates, the auxiliaries which can be used are: emulsifiers such as Nongru 500#, Nongru 700#, Nongru 2201, span-60 #, emulsifiers T-60, TX-10, Nongru 1601#, Nongru 600# and Nongru 400 #; solvents such as xylene, mineral spirits, toluene, biodiesel, methyl esterified vegetable oils, N-methyl pyrrolidone; cosolvents such as ethyl acetate, sec-butyl acetate, methanol, dimethylformamide, cyclohexanone, acetone, methyl ethyl ketone; stabilizers such as triphenyl phosphite, epichlorohydrin, acetic anhydride.

For aqueous emulsions, the auxiliaries which can be used are: emulsifier such as nonylphenol polyoxyethylene ether phosphate, triphenylethylphenol polyoxyethylene ether phosphate, Nongru No. 700, Nongru No. 2201, span-60, Tween-60, TX-10, Nongru 1601, Nongru No. 600, Nongru No. 400; one or more solvents such as xylene, toluene, cyclohexanone and solvent oil; one or more stabilizers such as triphenyl phosphite, epichlorohydrin and epoxidized soybean oil; an antifreezing agent: one or more of ethylene glycol, propylene glycol, glycerol, urea, and inorganic salts such as sodium chloride; thickening agent such as one or more of xanthan gum, polyvinyl alcohol, bentonite, and magnesium aluminum silicate; the antiseptic is one or more of formaldehyde, benzoic acid, and sodium benzoate, and the water is deionized water.

For microemulsions, the auxiliaries which can be used are: emulsifier Nongru 500#, Nongru 700#, Nongru 2201, span-60 #, Tween 60#, TX-10, Nongru 1601, Nongru 600#, Nongru 400 #; the auxiliary emulsifier is selected from methanol, isopropanol, n-butanol, and ethanol; the solvent is selected from cyclohexanone, N-methyl pyrrolidone, xylene, toluene and solvent oil; the stabilizer is selected from triphenyl phosphite and epoxy chloropropane; the water is deionized water.

For wettable powders, the auxiliaries which can be used are: dispersing agents such as one or more of polycarboxylate, lignosulfonate, polyoxyethylene polyoxypropylene ether block copolymer, alkyl naphthalene formaldehyde condensate sulfonate, alkylphenol polyoxyethylene ether phosphate, fatty alcohol polyoxyethylene ether phosphate and alkylphenol polyoxyethylene ether sulfonate; wetting agents such as one or more of alkyl sulfate, alkyl sulfonate, alkyl naphthalene sulfonate; the filler is selected from one or more of ammonium sulfate, urea, sucrose, glucose, diatomaceous earth, kaolin, white carbon black, light calcium carbonate, pulvis Talci, attapulgite, and pottery clay.

For water dispersible granules, the following additives can be used: a dispersant such as one or more of polycarboxylate, lignosulfonate, alkylnaphthalene sulfonate; wetting agents such as one or more of alkyl sulfate, alkyl sulfonate, naphthalene sulfonate; disintegrating agent such as one or more of ammonium sulfate, sodium sulfate, polyvinylpyrrolidone, starch and its derivatives, and bentonite; binder such as one or more of starch, glucose, polyvinyl alcohol, polyethylene glycol, sodium carboxymethylcellulose, and sucrose; the filler is one or more of diatomite, kaolin, white carbon black, light calcium carbonate, talcum powder, attapulgite and pottery clay.

For dispersible agents, the auxiliaries that can be used are: solvents such as one or more of dimethyl phthalate, dibutyl phthalate, ethyl acetate, methyl benzoate, acetone, alkyl pyrrolidone, ethanol, isopropanol, isobutanol; emulsifier such as one or more of Nongru 500#, Nongru 700#, Nongru 2201, span-60 #, emulsifier T-60, polyoxyethylene octylphenol ether, Nongru 1601#, Nongru 600# and Nongru 400 #; vegetable oil methyl ester such as palm oil methyl ester, coconut oil methyl ester, soybean oil methyl ester, and methyl oleate.

For suspension of microcapsules, the auxiliaries which can be used are: high molecular capsule wall material such as one or more of polyfunctional acyl halide polyamine, gelatin, acacia, modified milk protein, and sodium alginate; dispersing agent such as fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene formaldehyde condensate sulfate, and alkylbenzene sulfonate; solvent such as one or more of fatty acid ester, oleum Lini, and methyl oleate; emulsifiers such as one or more of benzyl dimethyl phenol polyoxyethylene ether, phenethyl phenol polyoxyethylene polypropylene ether, and ethylene oxide-propylene oxide block copolymer; pH regulator such as sodium hydroxide and one or more of hydrochloric acid, citric acid, and sorbic acid; defoaming agents such as one or more of C8-10 fatty alcohols, amides, silicone defoamers, hexanols, butanols, octanols; the water is deionized water.

The types of the auxiliary agents of various application formulations of the pesticide composition and the production process belong to the prior known technology, and are not described again.

The invention can achieve the following technical effects:

1. the two compounded active ingredients have different action mechanisms, and can delay the generation of drug resistance of pests;

2. after the two compounds are compounded, the synergistic effect is obvious, the using amount of pesticides can be reduced, and the cost is reduced.

Detailed Description

Indoor toxicity activity of mixing of the nitenpyram and the pyriproxyfen on the trialeurodes vaporariorum and the thrips is illustrated by indoor toxicity measurement.

1. Indoor toxicity determination method for trialeurodes vaporariorum by mixing of nitrofos and pyriproxyfen

The test method comprises the following steps: firstly, preparing the liquid medicine to be detected into the required concentration for standby. Firstly, beating fresh cucumber leaves into butterfly leaves, then immersing the beaten butterfly leaves in a liquid medicine to be detected for 10s, taking out and drying the butterfly leaves, flatly spreading the butterfly leaves with the front faces downward into a flat-bottom glass tube filled with agar by using tweezers, then putting the glass tube filled with the butterfly leaves on host cucumber leaves, slightly beating the cucumber leaves to enable whiteflies to enter the glass tube, enabling the whiteflies to climb into the butterfly leaves at the bottom of the tube by about 25 heads of the glass tube, downwards arranging the tube openings, enabling the whiteflies to climb into the glass tube openings, plugging the glass tube openings by using tampons, checking the death condition of the test insects in each tube after treatment for 48h, respectively recording the number of the test insects and the number of dead insects, and calculating the corrected death rate of each treatment.

2. Indoor toxicity determination of mixing of nitrofos and pyriproxyfen on thrips

The test method comprises the following steps: firstly, preparing the liquid medicine to be detected into the required concentration for standby. Soaking cotton leaf with thrips in the medicinal liquid for 8s, taking out, sucking off the excessive medicinal liquid with filter paper, transferring the cotton thrips nymph to normal condition for breeding, repeating the soaking for 4 times, and repeating the soaking for 15 times, wherein the treatment without medicament is used as blank control. And (5) checking the death condition of the test insects after 24h, respectively recording the number of the test insects and the number of the dead insects, and calculating the corrected death rate of each treatment.

Using co-virulent linesThe combined action of the insecticidal composition containing the nitre-phos provided by the invention on the trialeurodes vaporariorum and the thrips is evaluated by a numerical method, a regression equation is solved according to the logarithm of the concentration of the medicament and the control effect rate value, and LC (liquid chromatography) of each treatment is calculated₅₀And (4) obtaining the co-toxicity coefficient. The combined action of the compound medicament on trialeurodes vaporariorum and thrips is evaluated by the co-toxicity coefficient>120 is expressed as synergistic effect, the co-toxicity coefficient is expressed as additive effect between 80 and 120, and the co-toxicity coefficient<80 is shown as antagonistic. The test results are shown in tables 1 and 2.

Mortality (%) = number of dead insects (eggs)/number of total insects (eggs) treated × 100

Corrected mortality (%) = (treatment mortality-blank)/(1-blank mortality) × 100

TABLE 1 toxicity test results of mixing of Nitrophos and pyriproxyfen against Trialeurodes vaporariorum nymph

As can be seen from the table 1, the synergistic effect is shown on the nymphs of the trialeurodes vaporariorum when the compounding ratio of the nitrafenthion to the pyriproxyfen is 20:1-1:10, wherein the co-toxicity coefficient is the largest when the compounding ratio is 3:1, and the synergistic effect is most obvious.

TABLE 1 toxicity test results of mixing of Nitrophos and pyriproxyfen against thrips larvae

As can be seen from the table 2, the synergistic effect is shown on the thrips larvae when the compounding ratio of the nifedipine to the pyriproxyfen is 20:1-1:8, wherein the co-toxicity coefficient is the largest when the compounding ratio is 4:1, and the synergistic effect is most obvious.

The present invention is further illustrated by the following formulation examples.

Formulation example 1

30% of nitrothion, 10% of pyriproxyfen, 7% of N-methyl pyrrolidone, 5% of cyclohexanone, 6% of alkylaryl polyoxyethylene polyoxypropylene ether, 6.5% of calcium dodecylbenzene sulfonate, 3% of sorbitan fatty acid ester polyoxyethylene ether and 100% of No. 200 solvent oil. The above-mentioned raw materials are mixed, stirred and dissolved completely so as to obtain 40% nitro insect sulfur phosphorus pyriproxyfen emulsifiable concentrate.

Formulation example 2

30% of nitrobenfop, 10% of pyriproxyfen, 3% of tween-60 #, 3% of agricultural emulsion 700#, 5% of tween-60 #, 5% of propylene glycol, 5% of solvent oil S-20010%, 2% of toluene, 2% of xanthan gum, 2% of sodium benzoate and deionized water added to 100%. The raw materials are mixed and emulsified at high speed in a shearing way to prepare the 30 percent aqueous emulsion of the nifediphos-pyriproxyfen.

Formulation example 3

30% of nitrobenfop, 10% of pyriproxyfen, 5% of cyclohexanone, 8% of N, N-dimethylacetamide, 6% of N-butyl alcohol, 5% of calcium dodecyl benzene sulfonate, 5% of alkylaryl polyoxyethylene polyoxypropylene ether, 5% of glycerol laurate polyoxyethylene ether and deionized water to make up to 100%. Mixing the above materials, and dissolving to obtain oil phase. Adding the oil phase into deionized water under stirring to obtain the 40% nitre sulfur-phosphorus-pyriproxyfen microemulsion.

Formulation example 4

40% of nitrothion, 10% of pyriproxyfen, 6% of sodium dibutylnaphthalene sulfonate, 8% of alkylphenol polyoxyethylene ether phosphate, 3% of sodium dodecyl sulfate and enough white carbon black to 100%. The materials are mixed evenly according to the proportion, and after superfine airflow pulverization, the 50 percent wettable powder of the nifediphos-pyriproxyfen can be prepared.

Formulation example 5

40% of nitrofos, 10% of pyriproxyfen, 7% of polycarboxylate, 7% of sodium lignosulphonate, 5% of methylene sodium bis-methyl naphthalene sulfonate, 3% of sodium dodecyl sulfate, 15% of glucose and 100% of kaolin. The materials are uniformly mixed according to a proportion, superfine airflow crushing is carried out, and then drying and screening are carried out after rotary extrusion granulation, thus obtaining the 50 percent aqueous dispersion granule of the nifediphos and the pyriproxyfen.

Biological example 1: field pesticide effect test for controlling trialeurodes vaporariorum

The inventor carried out the efficacy test of the formulation example 1, the formulation example 2, the formulation example 3, the formulation example 4, the formulation example 5 and the control medicament for controlling the trialeurodes vaporariorum on cucumber in 2021, the test was carried out in the greenhouse of cucumber, and the test results are shown in table 3.

The test method comprises the following steps: every small area is 3X 6 square meter, and its periphery has protection rows, every treatment is repeated for 4 times, and every small area is randomly arranged, and its blank comparison is also set. The pesticide is applied when the trialeurodes vaporariorum is active and enough larvae are on each cucumber plant, and the pesticide application method is spraying. The base number was investigated before the treatment, and investigation was carried out once for each of 1d, 3d, 7d, and 15d after the treatment. During investigation, 10 cucumbers are fixed at each cell, 10 leaves are fixed at the upper, middle and lower parts of each cucumber, and the number of the live adults on the 10 cucumber plants in each cell is counted. And simultaneously observing whether the phenomenon of phytotoxicity occurs.

The drug effect calculation method comprises the following steps:

oral cavity decline rate (%) = (number of insects before application-number of insects after application)/number of insects before application × 100

Control effect (%) = (treatment area population reduction rate-control area population reduction rate)/(1-control area population reduction rate) × 100

TABLE 3 control Effect on greenhouse whitefly in cucumber

As can be seen from the table 3, the control effect of the compound preparation on the greenhouse trialeurodes vaporariorum in 1 day, 3 days, 7 days and 15 days after the pesticide is applied exceeds the control effect of each single agent, and the compound preparation has obvious synergistic effect on the control effect of the greenhouse trialeurodes vaporariorum.

During the test period, the cucumber with all the test agents grows safely without causing the phenomenon of phytotoxicity.

Biological example 2: field efficacy test for controlling thrips

The inventor carries out field efficacy tests on the cotton thrips by using the preparation example 1, the preparation example 2, the preparation example 3, the preparation example 4, the preparation example 5 and the control medicament in 2021, and the test results are shown in Table 4.

The test method comprises the following steps: the test cells are 20 square meters, the treatment is repeated for 4 times, blank contrast is additionally arranged, random blocks of the cells are arranged, and the cultivation conditions of the cells are uniform and consistent. Base number investigation was conducted before treatment, and application was conducted when the base number of thrips was not less than 300, the application method was spraying, and investigation was conducted once at 3d, 7d, and 14d after treatment. Sampling at five points in each cell, fixing 20 leaves on the middle upper part of the cotton, and investigating the number of live insects.

The drug effect calculation method comprises the following steps:

oral cavity decline rate (%) = (number of insects before application-number of insects after application)/number of insects before application × 100

Control effect (%) = (treatment area population reduction rate-control area population reduction rate)/(1-control area population reduction rate) × 100

TABLE 4 prevention and treatment of thrips on Cotton

As can be seen from Table 4, the control effect of the compound preparation on the cotton thrips in 3 days, 7 days and 14 days after the application exceeds the control effect of each single agent, and the compound preparation has obvious synergistic effect on the control effect of the cotton thrips.

During the test period, all the test agents are safe to the growth of cotton and have no phytotoxicity.

From the above examples, it can be known that the combination of the nifedipine and the pyriproxyfen according to the weight ratio of 20:1-1:10 can be made into various agriculturally acceptable dosage forms. Has obvious control effect on agricultural pests, obvious synergistic effect, reduced dosage and medication cost, can delay the generation of drug resistance of the pests, and is safe to crops.