阅读说明：本技术 一种天然肉桂精油消毒液及其制备方法 (Natural cinnamon essential oil disinfectant and preparation method thereof ) 是由 樊宏宇 陈波 钱胜红 于 2020-06-08 设计创作，主要内容包括：本发明提供了一种天然肉桂精油消毒液,按照重量百分比由以下原料组成：肉桂精油：1%～5.0%、比重调节剂：1%～4.5%、助表面活性剂：3%～8%、表面活性剂：4%～12%、去离子水：余量。本发明还提供了该消毒液的制备方法。本发明的消毒液能够杀灭多种致病菌、人畜可以安全使用、对皮肤无刺激性、无毒副作用、对环境无污染,且具有天然肉桂的气味。可以对皮肤、衣物、餐具、家居等物体表面进行消毒,满足人们的普遍需求。(The invention provides a natural cinnamon essential oil disinfectant, which comprises the following raw materials in percentage by weight: cinnamon essential oil: 1% -5.0%, specific gravity regulator: 1% -4.5%, cosurfactant: 3% -8%, surfactant: 4% -12%, deionized water: and (4) the balance. The invention also provides a preparation method of the disinfectant. The disinfectant disclosed by the invention can kill various pathogenic bacteria, can be safely used by people and livestock, has no irritation to skin, no toxic or side effect and no pollution to environment, and has the smell of natural cinnamon. Can disinfect the surfaces of objects such as skin, clothes, tableware, home furnishing and the like, and meets the general requirements of people.)

1. a natural cinnamon essential oil disinfectant comprises the following raw materials in parts by weight: cinnamon essential oil: 1% -5.0%, specific gravity regulator: 1% -4.5%, cosurfactant: 3% -8%, surfactant: 4% -12%, deionized water: and (4) the balance.

2. The method for preparing natural cinnamon essential oil disinfectant as claimed in claim 1, which is characterized by comprising the following steps:

1) adding cosurfactant into the cinnamon essential oil, and stirring until the cosurfactant is completely dissolved;

2) adding a specific gravity regulator, and stirring until the specific gravity regulator is completely dissolved;

3) adding a surfactant, and stirring until the surfactant is completely dissolved;

4) adding the rest of deionized water, and stirring until the system is clear.

3. Thirdly, the natural cinnamon essential oil disinfectant disclosed by the invention comprises the following steps of:

the specific gravity regulator in the step 1) is one or more selected from isopropyl palmitate, isopropyl myristate, isopropyl laurate and isopropyl stearate, and is preferably isopropyl laurate.

4. In particular, the specific gravity regulator is 1 to 4.5 percent by weight, preferably 2.4 percent by weight;

step 2) the cosurfactant disclosed by the invention is selected from one or a combination of more of isopropanol, glycerol and 1, 2-propylene glycol and ethanol, or ethanol is used alone, and preferably ethanol is used.

5. In particular, the cosurfactant is 3-8% by weight, preferably 4% by weight;

step 3) the surfactant provided by the invention is selected from one or more of PEG-40 hydrogenated castor oil (CO-40), polyethylene glycol hydrogenated castor oil (HEL-40) and castor oil polyoxyethylene ether (EL-40), and preferably PEG-40 hydrogenated castor oil (CO-40).

6. In particular, the cosurfactant is present in a proportion of 4% to 12% by weight, preferably 8% by weight.

Technical Field

The invention belongs to the field of medicines, and particularly relates to a natural cinnamon essential oil disinfectant and a preparation method thereof.

Background

Along with the continuous progress of society, the living standard of people is increasingly improved, and the health consciousness is also continuously enhanced. Meanwhile, with the rapid development of industry, the environment in which human beings live is also affected, and the occurrence of various novel bacteria and viruses increases the probability of human beings suffering from diseases. The active ingredients of the disinfection solution sold in the market at present mainly comprise peroxyacetic acid, 75% ethanol, formaldehyde, benzalkonium bromide and the like, and the disinfection solution has irritation and toxic and side effects and is not suitable for being generally used in places such as families and the like and being used for a long time. Therefore, an efficient and safe disinfectant suitable for long-term use in daily life is urgently needed to meet the requirements of people on sanitation and safety. Cinnamon is also known as cinnamon, cinnamon bark, cinnamon of the Lauraceae family (Latin's name: Cinnamomum cassia Presl), tropical evergreen arbor. Mainly distributed in Fujian, Taiwan, Hainan, Guangdong, Guangxi, Yunnan and other places. The essential oil obtained by distilling bark, branch and leaf of cinnamon has the effects of dispelling wind-damp, warming and strengthening spleen and stomach. It is commonly used for treating rheumatism, skin pruritus, yang collapse of spleen and stomach, cold limbs, slight pulse, cold accumulation of stomach and abdomen, abdominal pain, diarrhea, cold hernia and dolphin. Modern pharmacological research shows that the cinnamon oil has a killing effect on escherichia coli, staphylococcus aureus, bacillus subtilis, candida albicans and aspergillus flavus, and has a strong inhibiting effect on typhoid bacillus, paratyphoid bacillus, cholera bacillus, shigella, pneumococcus, bacillus anthracis, salmonella enteritidis, aerobacter, proteus and the like.

Cinnamon is also known as cinnamon, cinnamon bark, cinnamon of the Lauraceae family (Latin's name: Cinnamomum cassia Presl), tropical evergreen arbor. Mainly distributed in Fujian, Taiwan, Hainan, Guangdong, Guangxi, Yunnan and other places. The essential oil obtained by distilling bark, branch and leaf of cinnamon has the effects of dispelling wind-damp, warming and strengthening spleen and stomach. It is commonly used for treating rheumatism, skin pruritus, yang collapse of spleen and stomach, cold limbs, slight pulse, cold accumulation of stomach and abdomen, abdominal pain, diarrhea, cold hernia and dolphin. Modern pharmacological research shows that the cinnamon oil has a killing effect on escherichia coli, staphylococcus aureus, bacillus subtilis, candida albicans and aspergillus flavus, and has a strong inhibiting effect on typhoid bacillus, paratyphoid bacillus, cholera bacillus, shigella, pneumococcus, bacillus anthracis, salmonella enteritidis, aerobacter, proteus and the like.

However, because cinnamon essential oil is insoluble in water and easily oxidized in air, the application of cinnamon essential oil is greatly limited by the properties. Therefore, it is necessary to provide a disinfectant solution using cinnamon essential oil as a sterilization component, which has the advantages of simple components, uniform and stable properties, convenient preparation, low cost and no environmental pollution. Therefore, it is necessary to provide a disinfectant solution using cinnamon essential oil as a sterilization component, which has the advantages of simple components, uniform and stable properties, convenient preparation, low cost and no pollution to the environment.

Disclosure of Invention

The invention aims to solve the technical problem of overcoming the existing difficulty and provides a natural cinnamon essential oil disinfectant and a preparation method thereof. The disinfectant is prepared from the essential oil of natural plant cinnamon, can be safely used by people and animals, has no corrosiveness, no toxic or side effect, and no pollution to the environment, can disinfect the surfaces of objects such as skin, clothes, tableware, home furnishing and the like, and meets the general requirements of people.

Firstly, the invention is realized by adopting the following technical scheme:

a natural cinnamon essential oil disinfectant is prepared from the following components in percentage by weight:

name (R)	Content (wt.)
		Cinnamon essential oil	1~5%
Surface active agent	4~12%
		Specific gravity regulator	1~4.5%
Cosurfactant	3~8%
		Deionized water	Balance of

Secondly, the preparation method of the natural cinnamon essential oil disinfectant comprises the following steps:

1) adding cosurfactant into the cinnamon essential oil, and stirring until the cosurfactant is completely dissolved;

2) adding a specific gravity regulator, and stirring until the specific gravity regulator is completely dissolved;

3) adding a surfactant, and stirring until the surfactant is completely dissolved;

4) adding the rest of deionized water, and stirring until the system is clear.

Thirdly, the natural cinnamon essential oil disinfectant disclosed by the invention comprises the following steps of:

the specific gravity regulator in the step 1) is one or more selected from isopropyl palmitate, isopropyl myristate, isopropyl laurate and isopropyl stearate, and is preferably isopropyl laurate. In particular, the specific gravity regulator is 1 to 4.5 percent by weight, preferably 2.4 percent by weight;

step 2) the cosurfactant disclosed by the invention is selected from one or a combination of more of isopropanol, glycerol and 1, 2-propylene glycol and ethanol, or ethanol is used alone, and preferably ethanol is used. In particular, the cosurfactant is 3-5% by weight, preferably 8%;

step 3) the surfactant provided by the invention is selected from one or more of PEG-40 hydrogenated castor oil (CO-40), polyethylene glycol hydrogenated castor oil (HEL-40) and castor oil polyoxyethylene ether (EL-40), and preferably PEG-40 hydrogenated castor oil (CO-40). In particular, the cosurfactant is present in a proportion of 4% to 12% by weight, preferably 8% by weight.

The preparation method has the advantages of easy control, simplicity, practicability and low cost, and is suitable for industrial large-scale production.

Detailed Description

The present invention is further described below by way of examples, which are merely illustrative of the present invention and should not be construed as limiting the scope of the invention in any way. In addition, the reagents and raw materials in the embodiments can be obtained from commercial sources, and if the reagents and raw materials are inexhaustible, the instructions of daily chemical and pharmaceutical industry regulatory agencies, manufacturer specifications of corresponding instruments and reagents, and the like can be referred to.

The preparation method comprises the following steps: the specific addition of the components was carried out in accordance with the following specific examples.

1. The disinfectant preparation test adopts different components and proportions to carry out the test, and concretely comprises the following steps:

example 1

Cinnamon essential oil: 2.8%, ethanol: 4%, isopropyl laurate: 2.4%, PEG-40 hydrogenated castor oil (CO-40): 8% and deionized water: and (4) the balance.

Example 2

Cinnamon essential oil: 2.5%, ethanol: 5%, isopropyl palmitate: 3%, PEG-40 hydrogenated Castor oil (CO-40): 8% and deionized water: and (4) the balance.

Example 3

Cinnamon essential oil: 2% and ethanol: 3%, isopropyl laurate: 3% polyethylene glycol hydrogenated castor oil (HEL-40): 8% and deionized water: and (4) the balance.

Example 4

Cinnamon essential oil: 3% and ethanol: 4%, isopropyl laurate: 1%, isopropyl palmitate: 1.5%, PEG-40 hydrogenated castor oil (CO-40): 8% and deionized water: and (4) the balance.

Example 5

Cinnamon essential oil: 3% and ethanol: 3%, isopropyl alcohol: 1%, isopropyl laurate: 1.5%, isopropyl myristate: 1.5%, PEG-40 hydrogenated castor oil (CO-40): 8% and deionized water: and (4) the balance.

Example 6

Cinnamon essential oil: 1% and ethanol: 4% and glycerol: 1%, isopropyl myristate: 2%, isopropyl palmitate: 2%, PEG-40 hydrogenated Castor oil (CO-40): 6% of castor oil polyoxyethylene ether (EL-40): 2% and deionized water: and (4) the balance.

2. The quantitative sterilization test of the suspension of the disinfectant refers to the quantitative sterilization test of the suspension specified in 2008 edition 'sterilization technical code', and the sterilization effect of the disinfectant prepared in the steps on escherichia coli (ATCC 8099), staphylococcus aureus (ATCC 6538) and candida albicans (ATCC 10231) is detected, specifically as follows:

1) selecting three strains of staphylococcus aureus, escherichia coli and candida albicans to respectively prepare bacterial suspensions according to the sterilization technical specification, wherein the concentration of the bacterial suspension is 1 multiplied by 10⁸cfu/mL~5×10⁸cfu/mL for standby;

2) taking a sterile centrifuge tube, adding 0.5mL of bacterial suspension, adding 0.5mL of 0.3% calf serum albumin, uniformly mixing, sucking 4.0mL of the disinfectant prepared in each embodiment by using a pipette gun, injecting into the disinfectant, rapidly and uniformly mixing, and timing;

3) the test bacteria and the disinfectant are acted for each preset time, 0.5mL of mixed solution of the test bacteria and the disinfectant is respectively absorbed and added into 4.5mL of sterilized neutralizer, and the mixture is uniformly mixed;

4) adding neutralizer into the mixed solution of the test bacteria and the disinfectant for 10min, sucking 1.0mL of the mixed solution, measuring the number of viable bacteria according to a viable bacteria culture counting method, and inoculating 2 plates to each tube of test solution. If the number of colonies growing on the flat plate is more, serial 10-time dilution can be carried out, and viable bacteria culture counting is carried out;

5) all test samples are cultured in a thermostat at 37 ℃, and the results are observed for 24h of bacterial culture and 48h of fungal culture;

6) the test was repeated 3 times, and the viable bacteria concentration (cfu/mL) of each group was calculated and converted to a logarithmic value (N), and then the killing logarithmic value was calculated according to the following formula:

log Kill (KL) = log of mean active bacteria concentration of control group (N0) -log of mean active bacteria concentration of test group (Nx). If the killing log value of each time is more than or equal to 5.00, the disinfection is judged to be qualified.

The test results are as follows: