阅读说明：本技术 一种抗菌消毒纳米纤维医用敷料及制备方法 (Antibacterial and disinfectant nanofiber medical dressing and preparation method thereof ) 是由 曹鼎 魏文诗 胡水 冯拥军 舒心 于 2021-10-14 设计创作，主要内容包括：本发明公开了一种抗菌消毒纳米纤维医用敷料及制备方法,涉及医用敷料技术领域,该医用敷料包括敷芯层,为多层结构,具有至少两层抗菌层和至少两层吸液层交替排列；抗菌层为包括聚已内酯、聚乙二醇、丙三醇、海藻酸钠和抗菌药物的聚乳酸纳米复合纤维；吸液层为包括聚乙烯醇、聚乙二醇、抗菌活性成分、海藻酸钠、羧甲基壳聚糖和表皮细胞生长因子的聚乙烯醇/聚乙二醇纳米复合纤维。本发明具有抗菌层和吸液层多层结构,能同时实现温和抗菌修复和吸液促进伤口愈合,止血效果良好,透气性、舒适性和顺应性以及渗液吸收性优异,具有一定机械强度,揭除过程中能保持完整性,而且带来的痛感轻,使用后可降解对环境友好。(The invention discloses an antibacterial and disinfectant nanofiber medical dressing and a preparation method thereof, and relates to the technical field of medical dressings, wherein the medical dressing comprises a dressing layer which is of a multilayer structure and is provided with at least two antibacterial layers and at least two liquid absorption layers which are alternately arranged; the antibacterial layer is polylactic acid nano composite fiber containing polycaprolactone, polyethylene glycol, glycerol, sodium alginate and antibacterial drugs; the liquid absorbing layer is polyvinyl alcohol/polyethylene glycol nano composite fiber comprising polyvinyl alcohol, polyethylene glycol, antibacterial active ingredients, sodium alginate, carboxymethyl chitosan and epidermal cell growth factors. The invention has a multilayer structure of an antibacterial layer and a liquid absorption layer, can realize mild antibacterial repair and imbibition to promote wound healing, has good hemostatic effect, excellent air permeability, comfort, compliance and imbibition, has certain mechanical strength, can keep integrity in the removal process, brings light pain, and is degradable after use and environment-friendly.)

1. An antibacterial and disinfectant nanofiber medical dressing is characterized by comprising a dressing layer;

the core applying layer is of a multilayer structure and is provided with at least two antibacterial layers and at least two liquid absorbing layers which are alternately arranged;

the antibacterial layer is a polylactic acid nano composite fiber comprising polycaprolactone, polyethylene glycol, glycerol, sodium alginate and antibacterial drugs;

the liquid absorbing layer is polyvinyl alcohol/polyethylene glycol nano composite fiber comprising polyvinyl alcohol, polyethylene glycol, antibacterial active ingredients, sodium alginate, carboxymethyl chitosan and epidermal cell growth factors.

2. The medical dressing of claim 1, wherein the mass ratio of the polycaprolactone/polyethylene glycol mixture to the glycerol to the sodium alginate to the antibacterial drug is (70-90): (10-12): (8-12): (0.1 to 1).

3. The medical dressing of claim 2, wherein the mass ratio of polycaprolactone to polyethylene glycol is 1: 1. 4: 1. 6: 1. 10: 1 or 20: 1.

4. the medical dressing according to any one of claims 1 to 3, wherein the mass ratio of the polyvinyl alcohol/polyethylene glycol mixed solution to the antibacterial active ingredient to the sodium alginate to the carboxymethyl chitosan to the epidermal growth factor is (50-80): (10-15): (5-10): (1-2): (4-8).

5. The medical dressing of any one of claims 1-4, wherein the antibacterial drug is one or more of ciprofloxacin, ampicillin, oxacillin, levofloxacin, doxycycline, oxytetracycline, lomefloxacin, ibuprofen, ofloxacin;

the antibacterial active component is a periplaneta americana rehabilitation new liquid.

6. The medical dressing of any one of claims 1-5, wherein one outer surface of the dressing layer is a wicking layer.

7. The medical dressing of claim 6, further comprising a base layer;

the substrate layer is connected with the other outer surface of the cladding layer and is used for bearing the cladding layer.

8. The medical dressing of claim 6 or 7, further comprising a protective layer;

the protective layer is connected with one outer surface of the compress core layer and used for packaging the compress core layer.

9. A preparation method of an antibacterial and disinfectant nanofiber medical dressing is characterized by comprising the following steps:

mixing a polycaprolactone/polyethylene glycol mixture, glycerol, sodium alginate and an antibacterial drug according to a mass ratio of (70-90): (10-12): (8-12): (0.1-1) adding the mixture into a solvent, and magnetically stirring the mixture for 1.5-2.5 hours at the temperature of 30-60 ℃ to uniformly disperse the mixture to obtain a first mixture; adding the first mixture into a rotary bottle of a rotary evaporator, starting a condenser and a heating pot, raising the temperature of the first mixture to 55-65 ℃ after the temperature of the condenser is reduced to-15-25 ℃, and starting a vacuum pump and the rotary bottle for rotary distillation for 10-15 minutes; discharging when the temperature in the rotating bottle is reduced to 35-40 ℃ to obtain the spinning solution of the antibacterial layer;

mixing a polyvinyl alcohol/polyethylene glycol mixed solution, an antibacterial active ingredient, sodium alginate, carboxymethyl chitosan and an epidermal cell growth factor according to a mass ratio of (50-80): (10-15): (5-10): (1-2): (4-8), stirring for 30-35 minutes at the temperature of 35 ℃ to obtain a uniform and transparent liquid absorption layer spinning solution;

adding the antibacterial layer spinning solution into a first injector and then installing the first injector on a first injection pump of an electrostatic spinning instrument, adding the liquid absorbing layer spinning solution into a second injector and then installing the second injector on a second injection pump of the electrostatic spinning instrument, sequentially and circularly starting the first injection pump and the second injection pump to carry out electrostatic spinning, forming a sandwich layer with a multilayer structure, wherein the antibacterial layer and the liquid absorbing layer are alternately arranged, on a substrate layer on a collector, and stripping the sandwich layer and the substrate layer from the collector after spinning is finished;

covering the liquid absorbing layer of the core layer on the protective layer downwards, rolling by using a roller press, applying a force of 130-180N to compound the protective layer on the liquid absorbing layer, pressing and cutting the outer frame according to the size specification of the required medical dressing to obtain the antibacterial and disinfectant nanofiber medical dressing sheet, and packaging and sterilizing the sheet to obtain the finished antibacterial and disinfectant nanofiber medical dressing.

10. The method according to claim 9, wherein the step of preparing the polyvinyl alcohol/polyethylene glycol mixed solution comprises:

mixing polyvinyl alcohol and polyethylene glycol according to a mass ratio of 2: 1 is dissolved in hot water of 80-100 ℃ and stirred for 9-11 hours to prepare a 10-20 wt% polyvinyl alcohol/polyethylene glycol mixed solution.

Technical Field

The invention relates to the technical field of medical dressings, in particular to an antibacterial and disinfectant nanofiber medical dressing and a preparation method thereof.

Background

In recent years, degradable materials have attracted increasing attention, both from the viewpoint of environmental protection and from the viewpoint of convenience in use. In particular, biomedical materials are widely researched to avoid the need of secondary operations, drug release and enzyme carriers. Among them, the degradable polyester materials have been the hot spots due to their characteristics of good biocompatibility, good mechanical strength, innocuity and easy discharge of the degradation products, especially Polycaprolactone (PCL) has received great attention.

The traditional dressings commonly used in clinic at present comprise various gauzes, cotton pads, other synthetic dressings and the like. The traditional dressing has low cost, wide raw material sources, soft texture and strong liquid absorption capacity, can protect the wound surface and is still widely applied up to now. For example, film-type and foam-type dressings are generally used clinically. The film type dressing has transparent appearance and is convenient to observe, but the dressing is easy to cause the accumulation of the exudate under the film after being absorbed and saturated, possibly induces or aggravates infection, and is only suitable for relatively clean wound surfaces. The foam dressing has good protection effect, strong heat preservation and moisture preservation capabilities and light dressing, but some dressings need to be additionally provided with fixing materials due to poor adhesion, and the dressings are generally opaque and difficult to observe the condition of the wound surface.

The prior art has a Chinese patent with an authorization publication number of CN109758602B, and discloses a preparation method of an antibacterial PLA/PBC/CS composite dressing, wherein the dressing is obtained by carrying out electrostatic spinning on spinning solution, and the spinning solution comprises a suction-permeation composition consisting of PBC, PLA and CS and an antibacterial composition. The prepared composite dressing has good mechanical strength and short degradation time, and the waste basically has no pollution to the environment, but the composite dressing does not solve the defect of poor adhesion, can not be completely attached to the wound and can not prevent scar hyperplasia.

The prior art is granted with publication number CN113101405A, which discloses a "protective wound dressing", which is obtained by electrospinning a spinning solution containing a composition I consisting of a mixture of β -cyclodextrin and an alcohol extract of morinda officinalis, and a composition II consisting of modified coffee carbon nanoparticles and fengyuan. The prepared composite dressing has higher imbibition capability, strong bacteriostatic capability, can quickly heal wounds, prevent scar hyperplasia and reduce pigmentation near the wounds, but the defect that the dressing is difficult to completely peel off after imbibition is not solved.

Disclosure of Invention

Therefore, in order to overcome the defects, the embodiment of the invention provides an antibacterial and disinfectant nanofiber medical dressing and a preparation method thereof, wherein a multilayer antibacterial layer and a liquid absorption layer are compounded by adopting an alternate electrospinning method, and meanwhile, mild antibacterial repair and liquid absorption are realized to promote wound healing, the hemostatic effect is good, the air permeability, the comfort and the compliance are excellent, the liquid absorption is excellent, the mechanical strength is certain, the completeness can be kept in the removing process, the pain is light, and the dressing is degradable after use and is environment-friendly.

Therefore, the antibacterial and disinfectant nanofiber medical dressing comprises a dressing layer;

the core applying layer is of a multilayer structure and is provided with at least two antibacterial layers and at least two liquid absorbing layers which are alternately arranged;

the antibacterial layer is a polylactic acid nano composite fiber comprising polycaprolactone, polyethylene glycol, glycerol, sodium alginate and antibacterial drugs;

the liquid absorbing layer is polyvinyl alcohol/polyethylene glycol nano composite fiber comprising polyvinyl alcohol, polyethylene glycol, antibacterial active ingredients, sodium alginate, carboxymethyl chitosan and epidermal cell growth factors.

Preferably, the mass ratio of the polycaprolactone/polyethylene glycol mixture to the glycerol to the sodium alginate to the antibacterial drug is (70-90): (10-12): (8-12): (0.1 to 1).

Preferably, the mass ratio of the polycaprolactone to the polyethylene glycol is 1: 1. 4: 1. 6: 1. 10: 1 or 20: 1.

preferably, the mass ratio of the polyvinyl alcohol/polyethylene glycol mixed solution to the antibacterial active ingredient to the sodium alginate to the carboxymethyl chitosan to the epidermal cell growth factor is (50-80): (10-15): (5-10): (1-2): (4-8).

Preferably, the antibacterial drug is one or more than two of ciprofloxacin, ampicillin, oxacillin, levofloxacin, doxycycline, oxytetracycline, lomefloxacin, ibuprofen and ofloxacin;

the antibacterial active component is a periplaneta americana rehabilitation new liquid.

Preferably, one outer surface of the wicking layer is a liquid absorbent layer.

Preferably, a substrate layer is also included;

the substrate layer is connected with the other outer surface of the cladding layer and is used for bearing the cladding layer.

Preferably, a protective layer is further included;

the protective layer is connected with one outer surface of the compress core layer and used for packaging the compress core layer.

The preparation method of the antibacterial and disinfectant nanofiber medical dressing comprises the following steps:

mixing a polycaprolactone/polyethylene glycol mixture, glycerol, sodium alginate and an antibacterial drug according to a mass ratio of (70-90): (10-12): (8-12): (0.1-1) adding the mixture into a solvent, and magnetically stirring the mixture for 1.5-2.5 hours at the temperature of 30-60 ℃ to uniformly disperse the mixture to obtain a first mixture; adding the first mixture into a rotary bottle of a rotary evaporator, starting a condenser and a heating pot, raising the temperature of the first mixture to 55-65 ℃ after the temperature of the condenser is reduced to-15-25 ℃, and starting a vacuum pump and the rotary bottle for rotary distillation for 10-15 minutes; discharging when the temperature in the rotating bottle is reduced to 35-40 ℃ to obtain the spinning solution of the antibacterial layer;

mixing a polyvinyl alcohol/polyethylene glycol mixed solution, an antibacterial active ingredient, sodium alginate, carboxymethyl chitosan and an epidermal cell growth factor according to a mass ratio of (50-80): (10-15): (5-10): (1-2): (4-8), stirring for 30-35 minutes at the temperature of 35 ℃ to obtain a uniform and transparent liquid absorption layer spinning solution;

adding the antibacterial layer spinning solution into a first injector and then installing the first injector on a first injection pump of an electrostatic spinning instrument, adding the liquid absorbing layer spinning solution into a second injector and then installing the second injector on a second injection pump of the electrostatic spinning instrument, sequentially and circularly starting the first injection pump and the second injection pump to carry out electrostatic spinning, forming a sandwich layer with a multilayer structure, wherein the antibacterial layer and the liquid absorbing layer are alternately arranged, on a substrate layer on a collector, and stripping the sandwich layer and the substrate layer from the collector after spinning is finished;

covering the liquid absorbing layer of the core layer on the protective layer downwards, rolling by using a roller press, applying a force of 130-180N to compound the protective layer on the liquid absorbing layer, pressing and cutting the outer frame according to the size specification of the required medical dressing to obtain the antibacterial and disinfectant nanofiber medical dressing sheet, and packaging and sterilizing the sheet to obtain the finished antibacterial and disinfectant nanofiber medical dressing.

Preferably, the step of preparing the polyvinyl alcohol/polyethylene glycol mixed solution comprises:

mixing polyvinyl alcohol and polyethylene glycol according to a mass ratio of 2: 1 is dissolved in hot water of 80-100 ℃ and stirred for 9-11 hours to prepare a 10-20 wt% polyvinyl alcohol/polyethylene glycol mixed solution.

The antibacterial and disinfectant nanofiber medical dressing and the preparation method provided by the embodiment of the invention have the following advantages:

1. by preparing the sandwich layer multilayer structure with the antibacterial layers and the liquid absorption layers which are alternately arranged, the problem that the sandwich layer multilayer structure cannot be completely attached to a wound surface is effectively solved, and the sandwich layer multilayer structure is excellent in air permeability, comfort, compliance and liquid seepage absorbability and has certain mechanical strength.

2. The multilayer structure is combined with the addition of antibacterial substances, antibacterial active ingredients, epidermal cell growth factors and the like, and simultaneously realizes mild antibacterial repair and imbibition to promote wound healing, has good hemostatic effect and effectively avoids scar hyperplasia.

3. The multi-layer structure is combined with the problem that the viscosity is reduced after saturated absorption, so that the dressing is difficult to completely peel off after imbibing can be effectively avoided, the completeness can be kept in the peeling process, and the pain is slight.

4. The dressing components can be degraded after being used, so that the dressing is environment-friendly, 3 kinds of carcinogens of polyvinylpyrrolidone are avoided, and the dressing safety is higher.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.

Fig. 1 is a flowchart showing a specific example of a method for manufacturing an antibacterial and antiseptic nanofiber medical dressing according to example 1 of the present invention;

FIG. 2 is a schematic view showing a specific example of an electrospinning apparatus including a syringe pump No. one in example 1 of the present invention;

FIG. 3(a) shows that the mass ratio of PCL to PEG in example 1 of the present invention is 1: 1 fiber morphology of the antimicrobial layer;

FIG. 3(b) shows that the mass ratio of PCL to PEG in example 1 of the present invention is 4: 1 fiber morphology of the antimicrobial layer;

FIG. 3(c) shows that the mass ratio of PCL to PEG in example 1 of the present invention is 6: 1 fiber morphology of the antimicrobial layer;

FIG. 3(d) shows that the mass ratio of PCL to PEG in example 1 of the present invention is 10: 1 fiber morphology of the antimicrobial layer;

FIG. 3(e) shows that the mass ratio of PCL to PEG in example 1 of the present invention is 20: 1 fiber morphology of the antimicrobial layer;

FIG. 3(f) is a fiber morphology chart of an antibacterial layer of pure PCL in example 1 of the present invention;

FIG. 4 shows that the mass ratio of PCL to PEG in example 1 of the present invention is 20: 1, surface wetting process diagram of the antibacterial layer;

FIG. 5(a) is a fiber morphology view showing a specific example of the antibacterial layer of the antibacterial antiseptic nanofiber medical dressing in example 2 of the present invention;

fig. 5(b) is a fiber topography diagram of a specific example of the liquid absorbent layer of the antibacterial antiseptic nanofiber medical dressing in example 2 of the present invention.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In describing the present invention, it is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. The terms "comprises" and/or "comprising," when used in this specification, are intended to specify the presence of stated features, integers, steps, operations, elements, and/or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, and/or groups thereof. The term "and/or" includes any and all combinations of one or more of the associated listed items. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

In addition, the technical features involved in the different embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.

Example 1

The embodiment provides a preparation method of an antibacterial and disinfectant nanofiber medical dressing, which comprises the following steps as shown in fig. 1:

s1, preparing an antibacterial layer spinning solution:

mixing a Polycaprolactone (PCL)/polyethylene glycol (PEG) mixture, glycerol, sodium alginate and an antibacterial drug according to a mass ratio of (70-90): (10-12): (8-12): (0.1-1) adding the mixture into a solvent, and magnetically stirring the mixture for 1.5-2.5 hours at the temperature of 30-60 ℃ to uniformly disperse the mixture to obtain a first mixture; adding the first mixture into a rotary bottle of a rotary evaporator, starting a condenser and a heating pot, raising the temperature of the first mixture to 55-65 ℃ after the temperature of the condenser is reduced to-15-25 ℃, starting a vacuum pump and the rotary bottle, carrying out rotary distillation for 10-15 minutes, and carrying out rotary distillation on a solvent in the first mixture to carry out residual monomer; discharging when the temperature in the rotating bottle is reduced to 35-40 ℃ to obtain the spinning solution of the antibacterial layer;

s2, preparing a liquid absorbing layer spinning solution:

mixing a polyvinyl alcohol (PVA)/polyethylene glycol (PEG) mixed solution, an antibacterial active ingredient, sodium alginate, carboxymethyl chitosan and an epidermal cell growth factor according to a mass ratio of (50-80): (10-15): (5-10): (1-2): (4-8), stirring for 30-35 minutes at the temperature of 35 ℃ to obtain a uniform and transparent liquid absorption layer spinning solution;

s3, preparing a coating layer through electrostatic spinning:

adding the antibacterial layer spinning solution into the first injector and then installing the first injector on a first injection pump of an electrostatic spinning instrument, as shown in fig. 2, setting the spinning parameters of the first injection pump as follows: spinning distance is 15cm, propelling speed is 8mL/h, spinning voltage is 20kV, reciprocating distance of a stepping motor is 10cm, reciprocating speed is 1000 mm/min, a first injection pump is started to carry out electrostatic spinning after parameters are set, and spinning fibers (antibacterial layers) are collected on a substrate layer on a collector;

the liquid absorbing layer spinning solution is added into a second injector and then is installed on a second injection pump of the electrostatic spinning instrument, and the spinning parameters of the second injection pump are set as follows: the spinning distance is 15cm, the advancing speed is 8mL/h and the spinning voltage is 23kV, and spinning fibers (liquid absorption layers) are collected on a collector and on a substrate layer of the spinning antibacterial layer;

then changing into a first injection pump to carry out electrostatic spinning, collecting spinning fibers on a substrate layer which is on the collector and has been spun with the liquid absorbing layer and the antibacterial layer, spinning the first injection pump and then spinning the second injection pump, and so on to form a multilayer composite film with the liquid absorbing layers and the antibacterial layers which are alternately arranged by alternately spinning, namely a core applying layer, and stripping the core applying layer and the substrate layer from the collector after spinning;

s4, dressing forming:

covering the liquid absorbing layer of the core layer on the protective layer downwards, rolling by using a roller press, applying a force of 130-180N to compound the protective layer on the liquid absorbing layer, pressing and cutting the outer frame according to the size specification of the required medical dressing to obtain the antibacterial and disinfectant nanofiber medical dressing sheet, and packaging and sterilizing the sheet to obtain the finished antibacterial and disinfectant nanofiber medical dressing.

Preferably, the mass ratio of Polycaprolactone (PCL) to polyethylene glycol (PEG) is 1: 1. 4: 1. 6: 1. 10: 1 or 20: fig. 3(a) to 3(f) show fiber morphology of the antibacterial layers prepared with different mass ratios. As shown in fig. 4, the surface wetting process of the antibacterial layer (polycaprolactone (PCL)/polyethylene glycol (PEG) mass ratio of 20/1) composite fiber film is very excellent.

Preferably, the solvent is one or more of trifluoroacetic acid, hexafluoroisopropanol, a NaCl solution and dichloromethane.

Preferably, the antibacterial drug is one or more than two of ciprofloxacin, ampicillin, oxacillin, levofloxacin, doxycycline, oxytetracycline, lomefloxacin, ibuprofen and ofloxacin.

Preferably, the viscosity of the antibacterial layer spinning solution is 5000-.

Preferably, the step of preparing the polyvinyl alcohol (PVA)/polyethylene glycol (PEG) mixed solution includes:

mixing PVA and PEG according to a mass ratio of 2: 1 is dissolved in hot water at 80-100 ℃ and stirred for 9-11 hours to prepare 10-20 wt% of PVA/PEG mixed solution.

Preferably, the antibacterial active ingredient is periplaneta americana rehabilitation new liquid.

Preferably, the base layer is glassine.

Preferably, the spinning time of each antibacterial layer or each liquid absorption layer is 2-6 hours.

Preferably, the antibacterial layer and the liquid absorption layer of the core applying layer are respectively provided with 3-6 layers.

Preferably, the protective layer comprises a PU, PE, PVC, EPTFE or PET film.

According to the preparation method of the antibacterial disinfection nanofiber medical dressing, the problem that the dressing layer is not completely attached to the wound surface is effectively solved by preparing the dressing layer multilayer structure with the antibacterial layers and the liquid absorption layers which are alternately arranged, and scar hyperplasia is effectively avoided by adding the antibacterial active ingredients and the epidermal cell growth factors. And because of the existence of multilayer structure, can overcome the problem that can not laminate with the surface of a wound completely, also can make the viscidity reduce after applying the saturated absorption of sandwich layer, can effectively avoid the problem that the dressing is difficult to peel off completely after imbibition. In addition, the nano-scale dispersion of the imbibing agent and the sodium alginate-containing sustained-release particles is realized by utilizing an electrostatic spinning method, the volatilization of the sodium alginate in the production process of the imbibing layer can be reduced, in the using process, the designed antibacterial layer absorbs the liquid overflowing from the wound, and the sodium alginate-containing sustained-release particles are slowly softened and dissolved, so that the antibacterial dressing has a sustained-release effect, the air permeability is obviously improved, the influence on the respiration of the wound is avoided, and the wound healing is accelerated. The price is low, the processing and the forming are easy, the product specification can be customized according to the requirement, and the method has huge application value and wide market prospect.

The following is a detailed description by way of several specific examples.

Example one

S1, preparing an antibacterial layer spinning solution:

mixing a PCL/PEG mixture, glycerol, sodium alginate and an antibacterial drug according to a mass ratio of 80: 9: 10: 1, adding the mixture into a solvent, wherein the mass ratio of PCL to PEG is 1/1, the antibacterial drugs are ciprofloxacin, ampicillin and doxycycline, the solvent is a mixed solution of NaCl solution and hexafluoroisopropanol, and magnetically stirring the mixture for 2 hours at the temperature of between 30 and 60 ℃ to uniformly disperse the mixture to obtain a first mixture; adding the first mixture into a rotary bottle of a rotary evaporator, starting a condenser and a heating pot, starting a vacuum pump and the rotary bottle when the temperature of the condenser is reduced to-20 ℃ and the temperature of the first mixture is increased to 60 ℃, and carrying out rotary distillation for 10-15 minutes to carry out rotary distillation on the solvent in the first mixture and carry out residual monomer; discharging when the temperature in the rotating bottle is reduced to 35-40 ℃ to obtain the spinning solution of the antibacterial layer;

s2, preparing a liquid absorbing layer spinning solution:

mixing PVA and PEG according to a mass ratio of 2: dissolving the PVA/PEG mixed solution into hot water at the temperature of 80-100 ℃ and stirring for 10 hours to prepare a 15 wt% PVA/PEG mixed solution, mixing the PVA/PEG mixed solution, the American cockroach rehabilitation new solution, sodium alginate, carboxymethyl chitosan and epidermal cell growth factor according to the mass ratio of 70: 12: 8: 2: 8, mixing, and stirring for 30 minutes at the temperature of 35 ℃ to obtain a uniform and transparent imbibition layer spinning solution with the viscosity of 15000 CPS;

s3, preparing a coating layer through electrostatic spinning:

adding the antibacterial layer spinning solution into a first injector, then installing the first injector on a first injection pump of an electrostatic spinning instrument, setting a spinning distance of 15cm, a propelling speed of 8mL/h and a spinning voltage of 20kV, setting a reciprocating distance of a stepping motor to be 10cm, setting a reciprocating speed to be 1000 mm/min, then starting the first injection pump to carry out electrostatic spinning, collecting fibers on a substrate layer on a collector, wherein the substrate layer adopts glassine silicone oil paper;

adding the liquid absorbing layer spinning solution into a second injector, then installing the liquid absorbing layer spinning solution on a second injection pump of an electrostatic spinning instrument, setting the spinning distance to be 15cm, the advancing speed to be 8mL/h and the spinning voltage to be 23kV, setting the spinning time to be 2 hours, and collecting the fibers on a substrate layer on a collector, on which an antibacterial layer is spun;

then, changing into a first injection pump for spinning, collecting fibers on a substrate layer which is spun with a liquid absorbing layer and an antibacterial layer on a collector, spinning for the first time and then spinning for the second time, and repeating the steps for spinning alternately, wherein the antibacterial layer and the liquid absorbing layer are spun for 3 layers, the spinning time of each layer is 2 hours, and after the spinning is finished, a composite film coated with a core layer and the substrate layer is stripped from the collector;

s4, dressing forming:

covering the liquid absorbing layer applied to the core layer downwards on the protective layer, selecting a PET film as the protective layer, rolling the PET film by using a rolling machine, applying 150N force to compound the protective layer on the liquid absorbing layer, pressing and cutting the outer frame according to the size specification of the required medical dressing to prepare the antibacterial sterilizing nanofiber medical dressing sheet, and packaging and sterilizing the sheet to obtain the finished antibacterial sterilizing nanofiber medical dressing.

Example two

S1, preparing an antibacterial layer spinning solution:

mixing a PCL/PEG mixture, glycerol, sodium alginate and an antibacterial drug according to a mass ratio of 80: 9: 10: 1, adding the mixture into a solvent, wherein the mass ratio of PCL to PEG is 6/1, the antibacterial drugs are ciprofloxacin, ampicillin and doxycycline, the solvent is a mixed solution of NaCl solution and hexafluoroisopropanol, and magnetically stirring the mixture for 2 hours at the temperature of between 30 and 60 ℃ to uniformly disperse the mixture to obtain a first mixture; adding the first mixture into a rotary bottle of a rotary evaporator, starting a condenser and a heating pot, starting a vacuum pump and the rotary bottle when the temperature of the condenser is reduced to-20 ℃ and the temperature of the first mixture is increased to 60 ℃, and carrying out rotary distillation for 10-15 minutes to carry out rotary distillation on the solvent in the first mixture and carry out residual monomer; discharging when the temperature in the rotating bottle is reduced to 35-40 ℃ to obtain the spinning solution of the antibacterial layer;

s2, preparing a liquid absorbing layer spinning solution:

mixing PVA and PEG according to a mass ratio of 2: dissolving the PVA/PEG mixed solution into hot water at the temperature of 80-100 ℃ and stirring for 10 hours to prepare a 15 wt% PVA/PEG mixed solution, mixing the PVA/PEG mixed solution, the American cockroach rehabilitation new solution, sodium alginate, carboxymethyl chitosan and epidermal cell growth factor according to the mass ratio of 70: 12: 8: 2: 8, mixing, and stirring for 30 minutes at the temperature of 35 ℃ to obtain a uniform and transparent imbibition layer spinning solution with the viscosity of 15000 CPS;

s3, preparing a coating layer through electrostatic spinning:

adding the antibacterial layer spinning solution into a first injector, then installing the first injector on a first injection pump of an electrostatic spinning instrument, setting a spinning distance of 15cm, a propelling speed of 8mL/h and a spinning voltage of 20kV, setting a reciprocating distance of a stepping motor to be 10cm, setting a reciprocating speed to be 1000 mm/min, then starting the first injection pump to carry out electrostatic spinning, collecting fibers on a substrate layer on a collector, wherein the substrate layer adopts glassine silicone oil paper;

adding the liquid absorbing layer spinning solution into a second injector, then installing the liquid absorbing layer spinning solution on a second injection pump of an electrostatic spinning instrument, setting the spinning distance to be 15cm, the advancing speed to be 8mL/h and the spinning voltage to be 23kV, setting the spinning time to be 2 hours, and collecting the fibers on a substrate layer on a collector, on which an antibacterial layer is spun;

then, a first injection pump is replaced for spinning, fibers are collected on a base layer which is on a collector and is spun with a liquid absorbing layer and an antibacterial layer, a second spinning is carried out after the first spinning is finished, the spinning is carried out alternately in the same way, 4 layers of the antibacterial layer and the liquid absorbing layer are spun, the spinning time of each layer is 2 hours, and after the spinning is finished, a composite film coated with a core layer and the base layer is stripped from the collector;

s4, dressing forming:

covering the liquid absorbing layer applied to the core layer downwards on the protective layer, selecting a PET film as the protective layer, rolling the PET film by using a rolling machine, applying 150N force to compound the protective layer on the liquid absorbing layer, pressing and cutting the outer frame according to the size specification of the required medical dressing to prepare the antibacterial sterilizing nanofiber medical dressing sheet, and packaging and sterilizing the sheet to obtain the finished antibacterial sterilizing nanofiber medical dressing.

Example three

S1, preparing an antibacterial layer spinning solution:

mixing a PCL/PEG mixture, glycerol, sodium alginate and an antibacterial drug according to a mass ratio of 80: 9: 10: 1, adding the PCL/PEG with the mass ratio of 20/1, the antibacterial drugs of lomefloxacin, ibuprofen and ofloxacin and the solvent of a mixed solution of trifluoroacetic acid and hexafluoroisopropanol into a solvent, and magnetically stirring the mixture for 2 hours at the temperature of between 30 and 60 ℃ to uniformly disperse the mixture to obtain a first mixture; adding the first mixture into a rotary bottle of a rotary evaporator, starting a condenser and a heating pot, starting a vacuum pump and the rotary bottle when the temperature of the condenser is reduced to-20 ℃ and the temperature of the first mixture is increased to 60 ℃, and carrying out rotary distillation for 10-15 minutes to carry out rotary distillation on the solvent in the first mixture and carry out residual monomer; discharging when the temperature in the rotating bottle is reduced to 35-40 ℃ to obtain the spinning solution of the antibacterial layer;

s2, preparing a liquid absorbing layer spinning solution:

mixing PVA and PEG according to a mass ratio of 2: dissolving the PVA/PEG mixed solution into hot water at the temperature of 80-100 ℃ and stirring for 10 hours to prepare a 15 wt% PVA/PEG mixed solution, mixing the PVA/PEG mixed solution, the American cockroach rehabilitation new solution, sodium alginate, carboxymethyl chitosan and epidermal cell growth factor according to the mass ratio of 70: 12: 8: 2: 8, mixing, and stirring for 30 minutes at the temperature of 35 ℃ to obtain a uniform and transparent imbibition layer spinning solution with the viscosity of 15000 CPS;

s3, preparing a coating layer through electrostatic spinning:

adding the antibacterial layer spinning solution into a first injector, then installing the first injector on a first injection pump of an electrostatic spinning instrument, setting a spinning distance of 15cm, a propelling speed of 8mL/h and a spinning voltage of 20kV, setting a reciprocating distance of a stepping motor to be 10cm, setting a reciprocating speed to be 1000 mm/min, then starting the first injection pump to carry out electrostatic spinning, collecting fibers on a substrate layer on a collector, wherein the substrate layer adopts glassine silicone oil paper;

adding the liquid absorbing layer spinning solution into a second injector, then installing the liquid absorbing layer spinning solution on a second injection pump of an electrostatic spinning instrument, setting the spinning distance to be 15cm, the advancing speed to be 8mL/h and the spinning voltage to be 23kV, setting the spinning time to be 2 hours, and collecting the fibers on a substrate layer on a collector, on which an antibacterial layer is spun;

then, a first injection pump is replaced for spinning, fibers are collected on a base layer which is on a collector and is spun with a liquid absorbing layer and an antibacterial layer, spinning is carried out for the first time, then spinning is carried out for the second time, the spinning is carried out by the same method, the antibacterial layer and the liquid absorbing layer are respectively spun for 5 layers, the spinning time of each layer is 2 hours, and after the spinning is finished, a composite film coated with a core layer and the base layer is stripped from the collector;

s4, dressing forming:

covering the liquid absorbing layer applied to the core layer downwards on the protective layer, selecting a PET film as the protective layer, rolling the PET film by using a rolling machine, applying 150N force to compound the protective layer on the liquid absorbing layer, pressing and cutting the outer frame according to the size specification of the required medical dressing to prepare the antibacterial sterilizing nanofiber medical dressing sheet, and packaging and sterilizing the sheet to obtain the finished antibacterial sterilizing nanofiber medical dressing.

The performance test of the antibacterial disinfection nano-fiber medical dressing is as follows: (1) and (3) moisture absorption detection: and (3) respectively taking the antibacterial and disinfectant nanofiber medical dressings prepared in the first example, the second example and the third example of 5cm multiplied by 5cm, soaking the dressings in deionized water for 10 minutes, and measuring the weight of the antibacterial and disinfectant nanofiber medical dressings before and after soaking to obtain the water absorption. (2) Antibacterial property: determined according to the YY/T0471.5-2004 contact wound dressing test method, part 5, antibacterial procedure. The test data are as follows:

performance index	Example one	Example two	Example three
				Water absorption%	57.8	75.3	64.5
Antibacterial effect%	95.34	98.93	98.25
				Water vapour transmission rate g/m2×24h	2000	2800	2300

As can be seen from the table, the water absorption, antibacterial property and water vapor transmission rate of the three examples are all very good.

Example 2

The embodiment provides an antibacterial and disinfectant nanofiber medical dressing, which can be prepared by the preparation method of the antibacterial and disinfectant nanofiber medical dressing of embodiment 1, and comprises a dressing layer;

the core applying layer is of a multilayer structure and is provided with at least two antibacterial layers and at least two liquid absorbing layers which are alternately arranged; for example, a first antibacterial layer, a second liquid absorption layer, a third antibacterial layer, a fourth liquid absorption layer and the like are alternately formed into the core applying layer;

the antibacterial layer is a polylactic acid nano composite fiber comprising Polycaprolactone (PCL), polyethylene glycol (PEG), glycerol, sodium alginate and antibacterial drugs; preferably, the obtained fiber morphology is shown in fig. 5(a) by adopting electrostatic spinning preparation. When in use, the antibacterial drug in the dressing is slowly released, so that the stimulation to the skin is reduced on the premise of ensuring the antibacterial effect;

the liquid absorbing layer is polyvinyl alcohol/polyethylene glycol nano composite fiber comprising polyvinyl alcohol (PVA), polyethylene glycol (PEG), an antibacterial active ingredient, sodium alginate, carboxymethyl chitosan and epidermal cell growth factors; preferably, the obtained fiber morphology is shown in fig. 5(b) by adopting electrostatic spinning preparation. Preferably, one outer surface of the wicking layer is a liquid absorbent layer. When the dressing is used, the outermost liquid absorbing layer is contacted with skin, epidermal cell growth factors contained in the outermost liquid absorbing layer can promote cell growth, and the liquid absorbing layer is good in hygroscopicity, so that the dressing can promote wound healing, and the use air permeability and the comfort of the dressing are improved.

Preferably, the antimicrobial antiseptic nanofiber medical dressing further comprises a substrate layer;

the substrate layer is connected with the other outer surface of the cladding layer and is used for bearing the cladding layer; especially when electrospinning the antibacterial layer and the liquid absorbent layer in the wicking layer, the collected spun fibers are carried on the substrate layer. Preferably, the other outer surface may be an antimicrobial layer or a liquid absorbent layer.

Preferably, the antibacterial antiseptic nanofiber medical dressing further comprises a protective layer;

the protective layer is connected with one outer surface of the compress core layer, namely the liquid absorption layer, and is used for packaging the compress core layer to play a role in packaging and protecting.

Preferably, the mass ratio of the Polycaprolactone (PCL)/polyethylene glycol (PEG) mixture to the glycerol to the sodium alginate to the antibacterial drug is (70-90): (10-12): (8-12): (0.1 to 1).

Preferably, the mass ratio of the polyvinyl alcohol (PVA)/polyethylene glycol (PEG) mixed solution to the antibacterial active ingredient to the sodium alginate to the carboxymethyl chitosan to the epidermal cell growth factor is (50-80): (10-15): (5-10): (1-2): (4-8).

Preferably, the mass ratio of Polycaprolactone (PCL) to polyethylene glycol (PEG) is 1: 1. 4: 1. 6: 1. 10: 1 or 20: 1.

preferably, the solvent is one or more of trifluoroacetic acid, hexafluoroisopropanol, a NaCl solution and dichloromethane.

Preferably, the antibacterial drug is one or more than two of ciprofloxacin, ampicillin, oxacillin, levofloxacin, doxycycline, oxytetracycline, lomefloxacin, ibuprofen and ofloxacin.

Preferably, the step of preparing the polyvinyl alcohol (PVA)/polyethylene glycol (PEG) mixed solution includes:

mixing PVA and PEG according to a mass ratio of 2: 1 is dissolved in hot water at 80-100 ℃ and stirred for 9-11 hours to prepare 10-20 wt% of PVA/PEG mixed solution.

Preferably, the antibacterial active ingredient is periplaneta americana rehabilitation new liquid.

Preferably, the base layer is glassine.

Preferably, the antibacterial layer and the liquid absorption layer of the core applying layer are respectively provided with 3-6 layers.

Preferably, the protective layer comprises a PU, PE, PVC, EPTFE or PET film.

According to the antibacterial disinfection nanofiber medical dressing, the antibacterial layer and the liquid absorption layer which are alternately combined in a multi-layer mode are adopted, the polylactic acid nano composite fiber is prepared by the antibacterial layer through an electrostatic spinning method, and antibacterial substances in the dressing are slowly released when the dressing is used, so that stimulation to skin is reduced on the premise that the antibacterial effect is guaranteed; the liquid absorption layer is prepared into the polyvinyl alcohol/polyethylene glycol nano composite fiber by adopting an electrostatic spinning method, and the polyvinyl alcohol/polyethylene glycol nano composite fiber contains components for promoting cell growth, so that the hygroscopicity of the dressing can be increased, the wound healing can be promoted, and the use air permeability and the comfort of the dressing can be improved; and 3 kinds of carcinogens, namely polyvinylpyrrolidone, are avoided, so that the dressing is higher in safety; the antibacterial layer is good in design air permeability and good in hygroscopicity in the using process, the antibacterial active ingredients in the liquid absorption layer are uniformly dispersed and slowly released, the irritation to skin is small, the comfort is excellent, and the pain can be obviously relieved when the dressing is uncovered due to the multi-layer structure design.

It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.