阅读说明：本技术 胶原纤维口香糖的制备方法 (Preparation method of collagen fiber chewing gum ) 是由 赵换英 宋立国 马龙 崔浩冉 李智超 于 2021-10-11 设计创作，主要内容包括：本发明涉及胶原蛋白新应用技术领域,具体涉及一种胶原纤维口香糖的制备方法。本发明包括：(1)将胶原纤维与辅料混合；(2)将混合后的胶原团经过滤机梳理纤维,然后置于冷库中贮存；(3)将步骤(2)所得的冷藏的胶原团压制成胶原片；(4)将胶原片放置于中和液中进行中和,然后进行干燥,表面形成膜,且边缘没有发干,即制得所述的胶原纤维口香糖；所述的辅料包括多糖胶体和TG酶。本发明设计科学合理,操作简单易行,充分拓展了胶原纤维的用途,制备的胶原基口香糖咀嚼感强,可吞咽,易清理。(The invention relates to the technical field of new application of collagen, in particular to a preparation method of collagen fiber chewing gum. The invention comprises the following steps: (1) mixing the collagen fibers with auxiliary materials; (2) combing the mixed collagen clusters with a filter to obtain fibers, and then storing the fibers in a refrigeration house; (3) pressing the refrigerated collagen clusters obtained in the step (2) into a collagen sheet; (4) placing the collagen sheet in a neutralizing solution for neutralization, and then drying to form a film on the surface without drying the edge, thereby preparing the collagen fiber chewing gum; the auxiliary materials comprise polysaccharide colloid and TG enzyme. The invention has scientific and reasonable design and simple and easy operation, fully expands the application of the collagen fiber, and the prepared collagen-based chewing gum has strong chewing feeling, can be swallowed and is easy to clean.)

1. A method of preparing a collagen fiber chewing gum, comprising: the method comprises the following steps:

(1) mixing the collagen fibers with auxiliary materials;

(2) combing the mixed collagen clusters with a filter to obtain fibers, and then storing the fibers in a refrigeration house;

(3) pressing the refrigerated collagen clusters obtained in the step (2) into a collagen sheet;

(4) placing the collagen sheet in a neutralizing solution for neutralization, and then drying to prepare the collagen fiber chewing gum;

the auxiliary materials comprise polysaccharide colloid and TG enzyme.

2. The method for preparing a collagen fiber chewing gum according to claim 1, wherein: the preparation method of the collagen fiber comprises the following steps:

cutting cattle second-layer skin, pickling with lime, washing with lime and acidifying, cleaning the skin material, washing to remove fiber interstitium, and rolling into slices by a defibrator to obtain the collagen fiber.

3. The method for preparing a collagen fiber chewing gum according to claim 1, wherein: the polysaccharide colloid is chitosan or CMC.

4. The method for preparing a collagen fiber chewing gum according to claim 1, wherein: in the step (2), the filtering pressure is 8-12MPa, and the filter cake is stored in a refrigerator at the temperature of 6-10 ℃.

5. The method for preparing a collagen fiber chewing gum according to claim 1, wherein: in the step (3), the thickness of the collagen sheet is 1-2 mm.

6. The method for preparing a collagen fiber chewing gum according to claim 1, wherein: in the step (4), neutralization is carried out in a neutralization solution for 2 to 4 seconds.

7. The method for preparing a collagen fiber chewing gum according to claim 1, wherein: in the step (4), the neutralization solution contains 1-3 wt% of baking soda and 12-18 wt% of glycerol solution.

8. The method for preparing a collagen fiber chewing gum according to claim 1, wherein: in step (4), drying is carried out at 35-45 ℃ for 5-15 minutes.

9. The method for preparing a collagen fiber chewing gum according to claim 1, wherein: the auxiliary materials also comprise a sweetening agent, peppermint oil and an emulsifying agent.

10. The method for preparing a collagen fiber chewing gum according to claim 1, wherein: the obtained collagen fiber chewing gum has water content of 88-89 wt% and pH of 5-6.

Technical Field

The invention relates to the technical field of new application of collagen, in particular to a preparation method of collagen fiber chewing gum.

Background

In the production of collagen casings, large amounts of collagen dough are used. The collagen clusters can produce some unqualified products in the production process, and the collagen clusters which are not beneficial to producing the collagen casing can be used for producing other byproducts. The collagen cluster contains collagen fibers, which have strong water absorption and toughness and are soft.

At present, most of chewing gum is made of natural gum or glycerin resin as gum base, syrup, mint, sweetener and the like are added for blending and pressing, and the chewing gum is chewed in the mouth. CN105580968A discloses a chewing gum containing collagen peptides, but the gum base is still a natural gum or a synthetic resin, and said collagen peptides are not said collagen fibers.

Disclosure of Invention

The technical problem to be solved by the invention is as follows: the preparation method of the collagen fiber chewing gum overcomes the defects of the prior art, has scientific and reasonable design and simple and easy operation, fully expands the application of the collagen fiber, and the prepared collagen fiber chewing gum has strong chewing feeling, can be swallowed and is easy to clean.

The preparation method of the collagen fiber chewing gum comprises the following steps:

(1) mixing the collagen fibers with auxiliary materials;

(2) combing the mixed collagen clusters with a filter to obtain fibers, and then storing the fibers in a refrigeration house;

(3) pressing the refrigerated collagen clusters obtained in the step (2) into a collagen sheet;

(4) placing the collagen sheet in a neutralizing solution for neutralization, and then drying to form a film on the surface without drying the edge, thereby preparing the collagen fiber chewing gum;

the auxiliary materials comprise polysaccharide colloid and TG enzyme.

The preparation method of the collagen fiber (similar to the previous process steps for preparing the collagen casing) comprises the following steps:

cutting cattle second-layer skin, pickling with lime (primary alkali expansion of collagen fiber), washing with lime and acidification (continuous acid expansion of collagen fiber), cleaning the skin, washing to remove fiber interstitium, and rolling into thin sheets by a defibrator to obtain the collagen fiber.

Preferably, the polysaccharide colloid is chitosan or CMC.

Preferably, in the step (2), the filtration pressure is 8-12MPa, and the filter cake is stored in a refrigerator at 6-10 ℃.

Preferably, in the step (3), the thickness of the collagen sheet is 1-2 mm.

Preferably, in step (4), neutralization is carried out in a neutralization solution for 2 to 4 seconds.

Preferably, in the step (4), the neutralization solution contains 1-3 wt% of baking soda and 12-18 wt% of glycerol solution.

Preferably, in step (4), drying is carried out at 35-45 ℃ for 5-15 minutes.

The auxiliary materials also comprise a sweetening agent, peppermint oil, an emulsifying agent, potassium sorbate and the like.

The collagen fiber chewing gum prepared by the invention has the water content of 88-89% by mass and the pH value of 5-6.

Pure collagen fibers are difficult to be mixed and kneaded into a uniform state, the polysaccharide colloid is added, so that collagen clusters can be better fused together, and the polysaccharide colloid also has good film forming property and is convenient for later-stage molding and fixing; the TG enzyme added in the invention can enable collagen molecules to be cross-linked with each other and intra-molecule to increase the strength and stability of the collagen.

In the preparation process, the temperature is high, and the collagen is easy to deform and sticky, so that the collagen clusters after the fibers are combed are stored in a refrigeration house; since the collagen has a pH of 2 to 3, the collagen sheet is neutralized by being placed in a neutralizing solution, specifically, sodium bicarbonate, and glycerin is soaked for the purpose of moisturizing and reducing the stickiness of the collagen dough surface.

Compared with the prior art, the invention has the following beneficial effects:

(1) the preparation method of the collagen fiber chewing gum has scientific and reasonable design and simple and easy operation, and fully expands the application of the collagen fiber;

(2) the collagen-based chewing gum prepared by the invention has elasticity in fresh mouth, particularly strong chewing feeling and no tooth sticking; with the prolonging of chewing time, the temperature in the oral cavity can lead the collagen fiber to have certain decomposability and have a slightly soft feeling;

(3) the collagen-based chewing gum prepared by the invention can be swallowed and also can be spitted, the collagen can be decomposed and absorbed in vivo as a nutrient substance after being swallowed, and the collagen can be easily cleaned even if being spitted out, and has poor adhesion with the ground.

Detailed Description

The present invention is further illustrated by the following examples.

Example 1

A method of preparing a collagen fiber chewing gum comprising the steps of:

cutting the cattle second-layer leather into small blocks of 15 cm, adding lime water accounting for 20% of the weight of the leather into the small blocks, wrapping ash in the lime water to pickle the leather for 20 days (primary alkali expansion of collagen fibers), washing the ash, and then adding hydrochloric acid to acidify the leather (continuous acid expansion of the collagen fibers), wherein the pH value of the acid liquid is 1.0 until the acidification is complete. Then washing to remove the fibrous interstitium. And rolling into sheets by a defibrator to obtain the collagen fibers. The collagen fiber water content is 81%.

Mixing the obtained collagen fiber with adjuvants, kneading to obtain collagen cluster, mixing CMC, sweetener, TG enzyme, glyceryl stearate and potassium sorbate in water, and mixing with collagen fiber. The collagen fiber is firstly added into the mixing and kneading machine, and the auxiliary materials are sprayed into the collagen fiber in the mixing and kneading process in a spraying mode.

Every 2500kg of collagen cluster contains 2000kg of collagen fiber, 200kg of water and 300kg of mixed auxiliary materials. The adjuvant comprises CMC 1.5kg, sweetener 30kg, TG enzyme 7.5kg, glyceryl stearate 0.9kg, and potassium sorbate 2.5 g.

Carding the mixed collagen clusters through a filter to obtain fibers, wherein the filtering pressure is 8MPa, and storing the fibers in a refrigerator at 6 ℃; the refrigerated collagen pellet was compressed into a collagen sheet having a thickness of 1 mm.

And (3) placing the collagen sheet in a neutralizing solution for neutralizing for 2 seconds, wherein the neutralizing solution contains 1 wt% of baking soda and 12 wt% of glycerol solution, and then drying at 35-45 ℃ for 15 minutes to form a film on the surface without drying the edges, so that the collagen fiber chewing gum is prepared.

The prepared collagen fiber chewing gum has smooth appearance surface, uniform color, 88 mass percent of water and 5 pH.

Example 2

A method of preparing a collagen fiber chewing gum comprising the steps of:

cutting the cattle second-layer leather into small blocks of 15 cm, adding lime water accounting for 20% of the weight of the leather into the small blocks, wrapping ash in the lime water to pickle the leather for 20 days (primary alkali expansion of collagen fibers), washing the ash, and then adding hydrochloric acid to acidify the leather (continuous acid expansion of the collagen fibers), wherein the pH value of the acid liquid is 1.0 until the acidification is complete. Then washing to remove the fibrous interstitium. And rolling into sheets by a defibrator to obtain the collagen fibers. The collagen fiber has a water content of 83%.

Mixing the obtained collagen fiber with adjuvants, kneading to obtain collagen cluster, mixing chitosan, oleum Menthae Dementholatum, TG enzyme, glyceryl stearate and potassium sorbate in water, and mixing with collagen fiber. The collagen fiber is firstly added into the mixing and kneading machine, and the auxiliary materials are sprayed into the collagen fiber in the mixing and kneading process in a spraying mode.

Every 2500kg of collagen cluster contains about 2000kg of collagen fiber, 200kg of water and 300kg of mixed auxiliary materials. The auxiliary materials comprise 1.8kg of chitosan, 25kg of peppermint oil, 7.5kg of TG enzyme, 0.9kg of glyceryl stearate and 2.5g of potassium sorbate.

Carding the mixed collagen clusters through a filter to obtain fibers, wherein the filtering pressure is 10MPa, and storing the fibers in a refrigerator at the temperature of 8 ℃; the refrigerated collagen pellet was compressed into a collagen sheet having a thickness of 2 mm.

And (3) placing the collagen sheet in a neutralizing solution for neutralization for 3 seconds, wherein the neutralizing solution contains 2 wt% of baking soda and 15 wt% of glycerol solution, and then drying at 45 ℃ for 5 minutes to form a film on the surface without drying the edges, so that the collagen fiber chewing gum is prepared.

The prepared collagen fiber chewing gum has smooth appearance surface, uniform color, 89% of water mass content and 6 of pH.

Example 3

A method of preparing a collagen fiber chewing gum comprising the steps of:

cutting the cattle second-layer leather into small blocks of 15 cm, adding lime water accounting for 20% of the weight of the leather into the small blocks, wrapping ash in the lime water to pickle the leather for 20 days (primary alkali expansion of collagen fibers), washing the ash, and then adding hydrochloric acid to acidify the leather (continuous acid expansion of the collagen fibers), wherein the pH value of the acid liquid is 1.0 until the acidification is complete. Then washing to remove the fibrous interstitium. And rolling into sheets by a defibrator to obtain the collagen fibers. The collagen fiber has 85% of water.

Mixing the obtained collagen fiber with adjuvants, kneading to obtain collagen cluster, mixing CMC, oleum Menthae Dementholatum, TG enzyme, glyceryl stearate and potassium sorbate in water, and mixing with collagen fiber. The collagen fiber is firstly added into the mixing and kneading machine, and the auxiliary materials are sprayed into the collagen fiber in the mixing and kneading process in a spraying mode.

Every 2500kg of collagen cluster contains about 2000kg of collagen fiber, 200kg of water and 300kg of mixed auxiliary materials. The auxiliary materials comprise 2.0kg of CMC, 25kg of peppermint oil, 8.0kg of TG enzyme, 0.9kg of glyceryl stearate and 2.5g of potassium sorbate.

Carding the mixed collagen clusters into fibers by a filter under the filtering pressure of 12MPa, and storing in a cold storage at 10 ℃; the refrigerated collagen pellet was compressed into a collagen sheet having a thickness of 2 mm.

And (3) placing the collagen sheet in a neutralizing solution for neutralization for 4 seconds, wherein the neutralizing solution contains 3 wt% of baking soda and 18 wt% of glycerol solution, and then drying at 40 ℃ for 10 minutes to form a film on the surface without drying the edges, so that the collagen fiber chewing gum is prepared.

The prepared collagen fiber chewing gum has smooth appearance surface, uniform color, 88 mass percent of water and 5 pH.

Comparative example 1

The other process parameters and steps are the same as in example 1, except that:

no polysaccharide colloid was added in this comparative example.

The final chewing gum is loose in structure and not easy to form.

Comparative example 2

The other process parameters and steps are the same as in example 1, except that:

no TG enzyme was added to this comparative example.

The final prepared chewing gum had a slightly less chewy feel.

Comparative example 3

The other process parameters and steps are the same as in example 1, except that:

during neutralization, no glycerol was added.

The final chewing gum is sticky and adheres to the packaging paper.

The chewing gum made by the example has smooth appearance and uniform color.

And (3) detecting the adhesive force: the device (2017203230729 device for detecting the surface adhesive force of collagen) in the patent is utilized to place the prepared micelle in a groove, a chuck on a tensile machine clamps a rope, the rope is connected with a chassis, the diameter of the chassis is smaller than that of the groove, the chassis just contacts the micelle placed in the groove by setting the downward moving distance of the chuck on the tensile machine, then the tensile machine is clicked to carry out the tensile test, the upper chuck automatically moves upwards to be separated from the micelle, the detected value is read, and the result is shown in the table 1.

TABLE 1

Item	Example 1	Example 2	Example 3	Comparative example 1	Comparative example 2	Comparative example 3
							Value of viscosity, N	0.52	0.45	0.54	0.55	0.6	3.2

And (3) scoring the chewing mouthfeel:

randomly searching 10 consumers of 20-30 years old and 10 consumers of 30-40 years old, voting the chewing mouth feel of the chewing gum prepared by the invention and the commercial chewing gum, and the voting result is shown in table 2.

TABLE 2