阅读说明：本技术 一种含有提高皮肤弹性脂质体的面霜组合物及其制备方法 (A facial cream composition containing liposome for improving skin elasticity and its preparation method ) 是由 杨帆 周紫燕 李沫 郭苗 周正 于 2021-11-12 设计创作，主要内容包括：本发明公开了一种含有提高皮肤弹性脂质体的面霜组合物及其制备方法。该面霜包含：0.1-16份的提高皮肤弹性的脂质体、0.1-28份的润肤剂、0.12-14.5份的乳化剂、0.15-10.5份的填充剂、0.24-22.4份的增稠剂、0.01-5份的防腐剂、0.02-3份的EDTA-2NA、0.015-0.65份的pH调节剂、0.02-0.5份的日用香精,余量为去离子水。所述提高皮肤弹性脂质体的主要组分包含：齿缘墨角藻提取物、棕榈酰三肽-5/盐生杜氏藻/透明质酸钠/维他命原B5复合物、燕窝酸。本发明包含提高皮肤弹性脂质体的面霜组合物其通过各组分的同时复配可以增强皮肤弹性和柔软性,使用脂质体作为功效成分的递送载体,不仅能保持功效成分的稳定性,也能够加强功效成分的透皮效率,增加了该脂质体提高皮肤弹性的能力。(The invention discloses a cream composition containing liposome for improving skin elasticity and a preparation method thereof. The face cream comprises: 0.1-16 parts of liposome for improving skin elasticity, 0.1-28 parts of emollient, 0.12-14.5 parts of emulsifier, 0.15-10.5 parts of filler, 0.24-22.4 parts of thickener, 0.01-5 parts of preservative, 0.02-3 parts of EDTA-2NA, 0.015-0.65 parts of pH regulator, 0.02-0.5 parts of daily essence and the balance of deionized water. The main components of the skin elasticity improving liposome comprise: fucus alveolii extract, palmitoyl tripeptide-5/Dunaliella salina/sodium hyaluronate/provitamin B5 complex, and nidulanic acid. The cream composition containing the liposome for improving the skin elasticity can enhance the skin elasticity and softness by compounding the components at the same time, and the liposome is used as a delivery carrier of the functional components, so that the stability of the functional components can be maintained, the transdermal efficiency of the functional components can be enhanced, and the capability of the liposome for improving the skin elasticity is increased.)

1. A cream composition containing liposome for improving skin elasticity is characterized by comprising the following components in parts by weight:

0.1-16 parts of liposome for improving skin elasticity;

84-99.9 parts of cosmetic auxiliary material matrix.

2. The cream composition containing skin elasticity improving liposomes according to claim 1, wherein the ratio of the main components of the skin elasticity improving liposomes is, in parts by weight: 0.1-6.3 parts of fucus extract, 0.1-4.4 parts of palmitoyl tripeptide-5/dunaliella salina/sodium hyaluronate/provitamin B5 complex, and 0.1-5.5 parts of cubilose acid.

3. The cream composition containing skin elasticity improving liposomes according to claim 1, wherein the cosmetic adjuvant base is composed of the following components in parts by weight:

0.1-28 parts of an emollient;

0.12-14.5 parts of emulsifier;

0.15-10.5 parts of a filler;

0.24-22.4 parts of thickening agent;

0.01-5 parts of preservative;

0.02-3 parts of EDTA-2 NA;

0.015-0.65 parts of pH regulator;

0.02-0.5 parts of daily essence;

the balance being deionized water.

4. The cream composition containing skin elasticity improving liposomes according to claim 1, wherein the cream composition is composed of the following components in parts by weight:

0.5-8 parts of liposome for improving skin elasticity, which mainly comprises the following components: 0.1-2 parts of fucus extract, 0.2-4 parts of palmitoyl tripeptide-5/dunaliella salina/sodium hyaluronate/provitamin B5 complex, and 0.1-2.4 parts of cubilose acid;

1.5-20.5 parts of an emollient;

2.2-10.2 parts of emulsifier;

0.55-8 parts of a filler;

4-20 parts of a thickening agent;

0.01-1 part of preservative;

0.02-1.5 parts of EDTA-2 NA;

0.05-0.5 part of pH regulator;

0.04-0.42 part of daily essence;

the balance being deionized water.

5. The cream composition containing skin elasticity enhancing liposomes according to any one of claims 1 to 4, wherein the skin elasticity enhancing liposomes are contained in the following main components in mass ratio:

fucus serratus extract: palmitoyl tripeptide-5/dunaliella salina/sodium hyaluronate/provitamin B5 complex: 1, bird's nest acid: (0.5-2.6): (0.1 to 0.5); wherein palmitoyl tripeptide-5: dunaliella salina: sodium hyaluronate: provitamin B5 complex ═ 1: (1-4): (1-4): (1-2.5).

6. The cream composition containing skin elasticity improving liposomes according to any one of claims 1 to 4, wherein the preparation method of the skin elasticity improving liposomes comprises the steps of:

1) weighing a certain amount of fucus rudis extract, palmitoyl tripeptide-5/dunaliella salina/sodium hyaluronate/provitamin B5 complex, and cubilose acid in phosphate buffer solution, and dissolving with ultrasound;

2) weighing a certain amount of phospholipid, dissolving cholesterol in a proper amount of diethyl ether, pouring the solution obtained in the step 1) into a diethyl ether solution of the phospholipid and the cholesterol, homogenizing at a high speed for 3-10 minutes, and removing the diethyl ether at 30 ℃;

3) adding a certain amount of mannitol, carrying out ultrasonic emulsification, filtering with a 0.22-micron filter membrane, cooling the filtrate to-20 ℃ at the speed of 1-3 ℃/min, and carrying out freeze drying for 24-48 h to obtain the liposome for improving the skin elasticity.

7. The cream composition comprising liposomes for improving skin elasticity of claim 6, wherein: the phospholipid comprises: natural phospholipids, such as soybean phospholipid, lecithin, cephalin; synthetic phospholipids, such as dipalmitoylphosphatidylcholine, dipalmitoylphosphatidylethanolamine, distearoylphosphatidylcholine; the mass ratio of the phospholipid to the cholesterol is (1-6): 1.

8. the cream composition comprising liposomes for improving skin elasticity of claim 6, wherein: the prepared liposome has the particle size of 0.025-0.2 μm.

9. The cream composition comprising liposomes for improving skin elasticity of claim 3, wherein:

the emollient is selected from one or more of squalane, ethylhexyl isononanoate, isohexadecane, cetyl ethylhexanoate, tocopherol acetate, polydimethylsiloxane, hexyldecanol, trehalose, octyldodecanol lauroyl glutamate, phytosterol, xylitol, hydrogenated polyisobutene, jojoba oil, polydecene, cetostearyl glucoside and behenyl alcohol;

the emulsifier is one or more selected from behenyl alcohol polyether-25, PEG-100 stearate, sorbitan olivetoleate, polysorbate 20, polysorbate-80, isocetyl stearyl alcohol polyether-20, cetearyl alcohol polyether-30, arachidyl alcohol, oleyl alcohol polyether-2, and glyceryl stearate;

the filler is selected from one or more of titanium dioxide, mica powder, talcum powder and silica;

the thickening agent is selected from one or more of acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, carbomer, agar, pectin, hyaluronic acid, xanthan gum and alginic acid;

the preservative is selected from one or more of phenoxyethanol, p-hydroxybenzoate, 1, 2-pentanediol, sodium benzoate, methylparaben and sorbic acid;

the pH regulator is selected from one or two of lactic acid and arginine.

10. The method for preparing a cream composition containing liposomes for improving skin elasticity according to any one of claims 1 to 9, wherein: the method comprises the following steps:

1) wetting and completely dispersing the thickening agent in water to obtain a mixed solution;

2) adding emollient, filler, preservative and EDTA-2NA into the mixed solution in the step 1), heating to 65 ℃, and homogenizing at 2500-;

3) adding an emulsifier, a daily essence and a pH regulator into the step 2), homogenizing at 2500-.

Technical Field

The invention relates to a skin care product and a preparation method thereof, belongs to the technical field of daily chemicals, and particularly relates to a cream composition containing liposome for improving skin elasticity and a preparation method thereof.

Background

With the increase of age, the collagen and the elastin in the facial skin of the people can be gradually reduced to cause facial relaxation, the skin loses elasticity, and the problems of facial support structure collapse, skin relaxation, wrinkles and the like are brought along.

The two main substances influencing the elasticity of the skin are collagen and elastin in the dermis, and the collagen fiber and the elastic fiber formed by the two substances are interwoven with each other to form an elastic reticular supporting structure in the skin, so that the skin is full and elastic. When aging occurs, the collagen fibers and the elastic fibers are broken and oxidized, the net structure is damaged, and then the skin loses elasticity and becomes loose and shriveled.

Meanwhile, when the skin is in a water-deficient state for a long time, the elasticity of epidermal cells can be lost; long-term irradiation of ultraviolet rays destroys collagen fibers and elastic fibers, and causes the skin to be flaccid, aged, wrinkled, and the like. Finally, bad daily work and rest are also one of the reasons for skin looseness, and when the people are in short sleep for a long time, fatigue and smoking are caused, cell repair capacity and metabolism capacity are weakened, so that collagen and elastin of the skin are lost.

To solve the problem of the impaired skin elasticity caused by these complex factors, scientific allocation of some effective components is required. In addition, since proteins supporting the skin are mainly located in the dermis layer of the skin, how to effectively deliver the efficacy ingredients into the skin local is also a problem to be solved in the industry.

Disclosure of Invention

The invention aims to provide a cream composition containing skin elasticity improving liposome, which can promote the synthesis of skin collagen and improve the skin elasticity and is greatly favored by consumers.

Another object of the present invention is to provide a method for preparing a cream composition containing liposomes for improving skin elasticity.

In order to achieve the purpose, the invention adopts the following technical scheme:

a cream composition containing skin elasticity improving liposome comprises the following components in parts by weight:

0.1-16 parts of liposome for improving skin elasticity;

84-99.9 parts of cosmetic auxiliary material matrix.

Further, the skin elasticity improving liposome comprises the following main components in parts by weight: 0.1-6.3 parts of fucus extract, 0.1-4.4 parts of palmitoyl tripeptide-5/dunaliella salina/sodium hyaluronate/provitamin B5 complex, and 0.1-5.5 parts of cubilose acid.

Further, the cosmetic auxiliary material matrix consists of the following components:

0.1-28 parts of an emollient;

0.12-14.5 parts of emulsifier;

0.15-10.5 parts of a filler;

0.24-22.4 parts of thickening agent;

0.01-5 parts of preservative;

0.02-3 parts of EDTA-2 NA;

0.015-0.65 parts of pH regulator;

0.02-0.5 parts of daily essence;

the balance being deionized water.

Preferably, the cream composition containing the skin elasticity improving liposome is characterized by comprising the following components in parts by weight:

0.5-8 parts of liposome for improving skin elasticity, which mainly comprises the following components: 0.1-2 parts of fucus extract, 0.2-4 parts of palmitoyl tripeptide-5/dunaliella salina/sodium hyaluronate/provitamin B5 complex, and 0.1-2.4 parts of cubilose acid;

1.5-20.5 parts of an emollient;

2.2-10.2 parts of emulsifier;

0.55-8 parts of a filler;

4-20 parts of a thickening agent;

0.01-1 part of preservative;

0.02-1.5 parts of EDTA-2 NA;

0.05-0.5 part of pH regulator;

0.04-0.42 part of daily essence;

the balance being deionized water.

Preferably, the cream composition containing the skin elasticity improving liposome comprises the following components in parts by weight:

5-7 parts of liposome for improving skin elasticity;

8-15 parts of an emollient;

3-8 parts of an emulsifier;

1-4 parts of a filler;

10-15 parts of a thickening agent;

0.6-1 part of preservative;

0.5-1 part of EDTA-2 NA;

0.3-0.5 parts of pH regulator;

0.1-0.4 parts of daily essence;

the balance being deionized water.

Preferably, the liposome containing skin elasticity improving liposome comprises the following main components in mass ratio: palmitoyl tripeptide-5/dunaliella salina/sodium hyaluronate/provitamin B5 complex: 1, bird's nest acid: (0.5-2.6): (0.1-0.5). Wherein palmitoyl tripeptide-5: dunaliella salina: sodium hyaluronate: provitamin B5 complex ═ 1: (1-4): (1-4): (1-2.5).

Preferably, the method for preparing the liposome containing skin elasticity improving agent comprises the following steps:

1) weighing a certain amount of fucus rudis extract, palmitoyl tripeptide-5/dunaliella salina/sodium hyaluronate/provitamin B5 complex, and cubilose acid in phosphate buffer solution, and dissolving with ultrasound;

2) weighing a certain amount of phospholipid, dissolving cholesterol in a proper amount of diethyl ether, pouring the solution obtained in the step 1) into a diethyl ether solution of the phospholipid and the cholesterol, homogenizing at a high speed for 3-10 minutes, and removing the diethyl ether at 30 ℃;

3) adding a certain amount of mannitol, carrying out ultrasonic emulsification, filtering with a 0.22-micron filter membrane, cooling the filtrate to-20 ℃ at the speed of 1-3 ℃/min, and carrying out freeze drying for 24-48 h to obtain the liposome for improving the skin elasticity.

Preferably, in the method for preparing the liposome containing the skin elasticity improving agent, the phospholipid comprises: natural phospholipids, such as soybean phospholipid, lecithin, cephalin; synthetic phospholipids such as dipalmitoylphosphatidylcholine, dipalmitoylphosphatidylethanolamine, distearoylphosphatidylcholine and the like. The mass ratio of the phospholipid material to the cholesterol is (1-6): 1.

specifically, the liposome prepared by the preparation method of the liposome for improving the skin elasticity has the particle size of 0.025-0.2 μm.

Preferably, the cream composition containing skin elasticity improving liposome, the emollient is selected from one or more of squalane, ethylhexyl isononanoate, isohexadecane, cetyl ethyl hexanoate, tocopherol acetate, polydimethylsiloxane, hexyldecanol, trehalose, octyldodecanol lauroyl glutamate, phytosterol, xylitol, hydrogenated polyisobutene, jojoba oil, polydecene, cetostearyl glucoside and behenyl alcohol.

The emulsifier is one or more selected from behenyl alcohol polyether-25, PEG-100 stearate, sorbitan olivetoleate, polysorbate 20, polysorbate-80, isocetyl stearyl alcohol polyether-20, cetearyl alcohol polyether-30, arachidyl alcohol, oleyl alcohol polyether-2, and glyceryl stearate;

the filler is selected from one or more of titanium dioxide, mica powder, talcum powder and silica;

the thickening agent is selected from one or more of acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, carbomer, agar, pectin, hyaluronic acid, xanthan gum and alginic acid;

the preservative is selected from one or more of phenoxyethanol, p-hydroxybenzoate, 1, 2-pentanediol, sodium benzoate, methylparaben and sorbic acid;

the pH regulator is selected from one or two of lactic acid and arginine.

Preferably, the method for preparing the cream composition containing the liposome for improving skin elasticity comprises the following steps:

1) wetting and completely dispersing the thickening agent in water to obtain a mixed solution;

2) adding emollient, filler, preservative and EDTA-2NA into the mixed solution in the step 1), heating to 65 ℃, and homogenizing at 2500-;

3) adding an emulsifier, a daily essence and a pH regulator into the step 2), homogenizing at 2500-.

By means of the technical scheme, the invention has the following beneficial effects:

1) the innovative functional components capable of improving the skin elasticity mainly comprise: fucus alveolii extract, palmitoyl tripeptide-5/Dunaliella salina/sodium hyaluronate/provitamin B5 complex, and nidulanic acid. The three functional components are compounded simultaneously, so that the synthesis of skin collagen and elastin can be promoted, a supporting structure in a skin dermis layer is protected, and meanwhile, moisture can be provided for the skin, so that the skin is full and elastic.

2) The preparation method of the liposome for improving the skin elasticity is a freeze-drying method, and the fucus rudis extract, the palmitoyl tripeptide-5/dunaliella salina/sodium hyaluronate/provitamin B5 compound and the bird's nest acid which are effective components are wrapped by the liposome and then used, so that the effective components can enter the skin more easily to take effect, and the utilization rate of the effective components is improved.

Drawings

FIG. 1 is a graph showing the effect of inhibiting nonenzymatic glycosylation using the liposomes of example 1;

FIG. 2 is the change in the skin elasticity R2 value after use of example 1;

FIG. 3 is the change in the skin elasticity R5 value after use of example 1;

FIG. 4 is the change in the skin elasticity R7 value after use of example 1;

FIG. 5 is the change in skin elasticity test value after the use of example 1 and comparative examples 1 to 7.

Detailed Description

The invention discloses a face cream composition containing liposome for improving skin elasticity. The facial cream comprises the following raw material components in parts by weight: 0.1-16 parts of skin elasticity improving liposome, 0.1-28 parts of emollient, 0.12-14.5 parts of emulsifier, 0.15-10.5 parts of filler, 0.24-22.4 parts of thickener, 0.01-5 parts of preservative, 0.02-3 parts of EDTA-2NA, 0.015-0.65 parts of pH regulator, 0.02-0.5 parts of daily essence and the balance of deionized water. All the raw material components are commercially available products.

The main components of the skin elasticity improving liposome comprise: fucus alveolii extract, palmitoyl tripeptide-5/Dunaliella salina/sodium hyaluronate/provitamin B5 complex, and nidulanic acid. The cream composition containing the liposome for improving the skin elasticity can enhance the skin elasticity and softness by compounding the components at the same time, wherein the fucus extract at the edge of the teeth can provide certain nutrition for the skin and promote the synthesis of collagen; the palmitoyl tripeptide-5/dunaliella salina/sodium hyaluronate/provitamin B5 compound can supplement skin moisture and hyaluronic acid to increase skin plumpness; the cubilose acid can effectively remove excessive free radicals in the skin so as to protect collagen and elastin in the dermis layer of the skin.

In addition, the liposome is used as a delivery carrier of the functional components, so that the stability of the functional components can be maintained, the transdermal efficiency of the functional components can be enhanced, and the capability of the composition for improving the skin elasticity is increased.

The invention discloses a face cream composition containing skin elasticity improving liposome. The cream composition mainly comprises the following functional components for improving the skin elasticity: fucus serratus extract, palmitoyl tripeptide-5/Dunaliella salina/sodium hyaluronate/provitamin B5 complex, and nidulanic acid, which have the effects of promoting skin collagen synthesis and improving skin elasticity. Through the preferable combination and efficacy test, the most preferable composition proportion is found to obtain the efficacies of synergistically enhancing, beautifying and firming the face and repairing the skin.

(ii) Fucus serratus extract

Fucus serratus is distributed in the North Atlantic ocean, growing from the semi-tidal level to low water levels, and is a well-known algae available to humans. The Fucus serratus extract is rich in various essential amino acids, and can be used in cosmetics for providing nutrition to skin. The Fucus serratus extract can maintain skin health, participate in collagen synthesis, promote cell metabolism, and make skin compact and elastic.

(II) palmitoyl tripeptide-5/Dunaliella salina/sodium hyaluronate/provitamin B5 complex

The hyaluronic acid of the dermal layer acts as an outer frame for structural proteins (e.g., collagen, elastin) and skin cells. The reduction of hyaluronic acid in the dermis results in loss of turgor and laxity of the skin, as well as reduced skin hydration. The selected effect compound can effectively supplement hyaluronic acid for skin, repair cells, smoothen skin, promote skin moisture retention, and achieve the effects of remarkably removing wrinkles and instantly making skin soft and elastic.

Wherein, palmitoyl tripeptide-5: dunaliella salina: sodium hyaluronate: provitamin B5 complex ═ 1: (1-4): (1-4): (1-2.5).

③ bird's nest acid

The bird's nest acid is widely existed in animal tissues, and is also contained in a plurality of foods, can participate in various skin cell physiological functions, improves the skin of people, and is favored by a plurality of skin care products nowadays. The cubilose acid can promote the synthesis of structural protein in a dermis layer, can effectively inhibit non-enzymatic glycosylation reaction, effectively protects the supporting structures in the dermis layer of the skin, namely collagen and elastin, reduces the elasticity of the skin, and enables the skin to be compact and smooth.

Examples 1 to 3

A facial cream composition containing skin elasticity improving liposome comprises the following raw material components in proportion as shown in the following table 1.

TABLE 1 raw material component ratios (unit: parts by mass) of examples 1 to 3

The process for preparing the cream containing the liposome for improving skin elasticity of example 1 includes the following steps:

1) preparing the skin-elasticity-improving liposome a: weighing 750mg of fucus rudis extract, 562mg of palmitoyl tripeptide-5/dunaliella salina/sodium hyaluronate/provitamin B5 compound and 375mg of cubilose acid in 20mL of phosphate buffer solution, and dissolving by ultrasonic; weighing 2500mg of phospholipid and 500mg of cholesterol, dissolving in 20mL of diethyl ether, pouring the solution in the phosphoric acid buffer solution dissolved with the functional components into the diethyl ether solution of the phospholipid, homogenizing at high speed for 5 minutes, and removing the diethyl ether at 30 ℃; adding 2% mannitol, ultrasonic emulsifying, filtering with 0.22 μm filter membrane, and freeze drying the filtrate at 3 deg.C/min to-20 deg.C for 40h to obtain liposome with skin elasticity improving effect, wherein the particle diameter of liposome is 0.13 + -0.03 μm.

2) Wetting and completely dispersing the thickening agent in water to obtain a mixed solution;

3) adding the emollient, the filler, the preservative and the EDTA-2NA into the mixed solution obtained in the step 2), heating to 65 ℃, and homogenizing at 3200r/min for 6 minutes to completely disperse the mixed solution;

4) adding an emulsifier, a daily essence and a pH regulator into the step 3), homogenizing at 3200r/min for 6 minutes to completely emulsify the mixture, and adding the liposome a while stirring and cooling to obtain the cream composition containing the skin elasticity improving liposome.

The preparation process of the cream containing the liposome for improving skin elasticity of the above example 2 includes the following steps:

1) preparing the skin elasticity improving liposome b: weighing 150mg of fucus rudis extract, 112mg of palmitoyl tripeptide-5/dunaliella salina/sodium hyaluronate/provitamin B5 complex and 75mg of cubilose acid in 10mL of phosphate buffer solution, and dissolving by ultrasonic; weighing 500mg of phospholipid and 250mg of cholesterol, dissolving in 10mL of diethyl ether, pouring the solution in the phosphoric acid buffer solution dissolved with the functional components into the diethyl ether solution of the phospholipid, homogenizing at high speed for 3 minutes, and removing the diethyl ether at 30 ℃; adding 1% mannitol, ultrasonic emulsifying, filtering with 0.22 μm filter membrane, and freeze drying the filtrate at 1 deg.C/min to-20 deg.C for 24 hr to obtain liposome with skin elasticity improving effect, wherein the liposome particle diameter is 0.15 + -0.04 μm.

2) Wetting and completely dispersing the thickening agent in water to obtain a mixed solution;

3) adding the emollient, the filler, the preservative and the EDTA-2NA into the mixed solution obtained in the step 2), heating to 65 ℃, and homogenizing at 3000r/min for 3 minutes to completely disperse the mixed solution;

4) adding an emulsifier, a daily essence and a pH regulator into the step 3), homogenizing at 3000r/min for 3 minutes to completely emulsify the mixture, and adding the liposome b while stirring and cooling to obtain the cream composition containing the skin elasticity improving liposome.

The process for preparing the cream containing the liposome for improving skin elasticity of the above example 3 includes the following steps:

1) preparing the skin-elasticity-improving liposome c: weighing 730mg of fucus rudis extract, 548mg of palmitoyl tripeptide-5/dunaliella salina/sodium hyaluronate/provitamin B5 compound and 365mg of cubilose acid in 30mL of phosphate buffer solution, and ultrasonically dissolving; weighing 3000mg of phospholipid and 1000mg of cholesterol, dissolving in 30mL of diethyl ether, pouring the solution in the phosphoric acid buffer solution dissolved with the functional components into the diethyl ether solution of the phospholipid, homogenizing at high speed for 8 minutes, and removing the diethyl ether at 30 ℃; adding 3% mannitol, ultrasonic emulsifying, filtering with 0.22 μm filter membrane, and freeze drying the filtrate at 2 deg.C/min to-20 deg.C for 48h to obtain liposome with skin elasticity improving effect, wherein the liposome particle diameter is 0.11 + -0.03 μm.

2) Wetting and completely dispersing the thickening agent in water to obtain a mixed solution;

3) adding the emollient, the filler, the preservative and the EDTA-2NA into the mixed solution obtained in the step 2), heating to 65 ℃, and homogenizing at 3500r/min for 8 minutes to completely disperse the mixed solution;

4) adding an emulsifier, a daily essence and a pH regulator into the step 3), homogenizing at 3500r/min for 8 minutes to completely emulsify the mixture, and adding the liposome c while stirring and cooling to obtain the cream composition containing the skin elasticity improving liposome.

Test example for Effect verification

1. Safety test

Test samples: example 1;

the test method comprises the following steps: and (4) testing the safety by adopting a third-party detection mechanism. The selection area is not more than 50mm²And a suitable patch device having a depth of about 1mm, wherein about 0.02 to about 0.025ml (g) of the test substance is applied to the patch device in a closed patch test. Sticking the spot tester to the forearm of the subject, removing the test substance 24h later, observing skin reaction 0.5h, 24h and 48h after removal, and recording according to skin reaction grading standard in' cosmetic safety technical regulation 2015As a result, the coating composition was cured.

TABLE 2 Patch test results

Remarking: grading standard of skin reaction

Level 0: negative reaction;

level 1: carrying out suspicious reaction; only faint erythema;

and 2, stage: weak positive reaction (erythema reaction); erythema, infiltration, edema, and possibly papules;

and 3, level: strong positive reaction (herpes reaction); erythema, infiltration, edema, pimples, herpes; the reaction may be beyond the test area;

4, level: very strong response (fusional herpes response); erythema, severe infiltration, edema, fusional herpes, and response beyond the test area.

As can be seen from table 2 above, the cream prepared showed no irritation and passed safety.

2. Stability test

Test samples: example 1;

the test method comprises the following steps: and (5) observing the appearance color, viscosity and pH value for three months according to internal control standard.

Table 3 stability test results

Time

45℃

40℃

37℃

25℃

5℃

-15℃

CYCLE

Illumination of light

1W

√

√

√

√

√

√

√

√

2W

√

√

√

√

√

√

√

√

3W

√

√

√

√

√

√

√

√

4W

√

√

√

√

√

√

√

√

2M

√

√

√

√

√

√

√

√

3M

√

√

√

√

√

√

√

√

As can be seen from the above table 3, the prepared cream has no significant changes in color, viscosity and pH value within three months under high and low temperature or illumination conditions, which indicates that the cream has better stability.

3. Efficacy testing of liposomes for improving skin elasticity

Test samples: the skin elasticity-improving liposome of example 1;

the test method comprises the following steps: and (3) adopting an in-vitro non-enzymatic glycosylation reaction experiment, and measuring the inhibition rate of the sample on non-enzymatic glycosylation by using a fluorescence spectrophotometer.

And (3) testing results: as shown in FIG. 1, the inhibition rate of the liposomes to non-enzymatic glycosylation gradually increased with the increase of the concentration of the liposomes, and reached 74% at the use amount of 5%. The liposome can obviously reduce non-enzymatic glycosylation reaction, reduce the damage of collagen and elastin at the local part of the skin, and has the functions of maintaining the skin supporting effect and protecting the elasticity of the skin.

4. Evaluation of skin elasticity-improving effect of cream

Test samples: example 1 (experimental group) and a base cream (control group: cream composition without liposome coating, other components content same as experimental group);

the test method comprises the following steps: the face cream is applied to the whole face after face cleaning every day, and the sunscreen product is applied to the whole face in the morning. The test was carried out in a room at 20-22 ℃ and 40-60% relative humidity, and the subjects had to be acclimated for 15 minutes in the same environment.

The results were tested at the following 4 time periods, respectively: before use of the sample (D0), after use of the sample for 14 days (D14), after use of the sample for 28 days (D28) and after use of the sample for 56 days (D56).

The detection items are as follows:

use ofMPA580 measures skin elasticity, with closer measurements to 1 indicating better skin elasticity. (R2 is the ratio of the amount of springback of the skin without negative pressure to the maximum amount of stretching during negative pressure; R5 is the ratio of the elastic portion of the skin recovery process during the first cycle of the skin test to the elastic portion of the skin recovery process during the application of negative pressure; R7 is the ratio of the elastic portion of the skin recovery process during the first cycle of the skin test to the maximum amount of stretching of the skin during this cycle).

And (3) testing results:

as shown in fig. 2 to 4 and tables 4 to 6, the significance of the R2, R5 and R7 values of the experimental group is greater than that of the control group, which indicates that the prepared cream can effectively improve the skin elasticity. The three components are wrapped by the liposome and then used simultaneously, so that the synthesis of skin collagen and elastin can be promoted, a supporting structure in the dermis layer of the skin is protected, and meanwhile, moisture can be provided for the skin, so that the skin is full and elastic.

TABLE 4 skin elasticity R2 values

Time	D0	D14	D28	D56
					Experimental group	0.569	0.593	0.635	0.686
Control group	0.556	0.558	0.574	0.587

TABLE 5 skin elasticity R5 values

Time	D0	D14	D28	D56
					Experimental group	0.516	0.586	0.612	0.674
Control group	0.521	0.536	0.547	0.565

TABLE 6 skin elasticity R7 values

Time	D0	D14	D28	D56
					Experimental group	0.366	0.391	0.426	0.512
Control group	0.310	0.346	0.323	0.378

5. Synergistic experiment of cream composition

The raw material components of the cream composition are in the proportion and content shown in the following table 7.

TABLE 7 ingredient proportion table (unit: parts by mass) of cream in comparative examples 1 to 7

The preparation process of the face cream comprises the following steps:

1) wetting and completely dispersing the thickening agent in water to obtain a mixed solution;

2) adding main functional components, an emollient, a filler, a preservative and EDTA-2NA into the mixed solution obtained in the step 1), heating to 65 ℃, and homogenizing at 3200r/min for 6 minutes to completely disperse the mixed solution;

3) adding an emulsifier, a daily essence and a pH regulator into the step 2), homogenizing at 3200r/min for 6 minutes to completely emulsify, stirring and cooling to obtain the cream composition.

The effects of example 1 and comparative examples 1 to 7 were verified (evaluation of skin elasticity improvement effect), and the experimental procedures and results were as follows:

1. the tester: 80 healthy women (25-45 years old) divided into 8 groups of 10 persons each; each group used the same formulation.

2. Use of: the face cream is applied to the whole face after face cleaning every day, and the sunscreen product is applied to the whole face in the morning. The test was carried out in a room at 20-22 ℃ and 40-60% relative humidity, and the subjects had to be acclimated for 15 minutes in the same environment. The results were tested at the following 4 time periods, respectively: before use of the sample (D0), after use of the sample for 14 days (D14), after use of the sample for 28 days (D28) and after use of the sample for 56 days (D56).

3. The detection items are as follows: use ofMPA580 measures skin elasticity, with closer measurements to 1 indicating better skin elasticity. (R2 is the ratio of the amount of springback of the skin without negative pressure to the maximum amount of stretching during negative pressure; R5 is the ratio of the elastic portion of the skin recovery process during the first cycle of the skin test to the elastic portion of the skin recovery process during the application of negative pressure; R7 is the ratio of the elastic portion of the skin recovery process during the first cycle of the skin test to the maximum amount of stretching of the skin during this cycle).

Δ R2 ═ R2 (measured after 8 weeks) -R2 (measured at week 0). Δ R5 ═ R5 (measured after 8 weeks) -R5 (measured at week 0). Δ R7 ═ R7 (measured after 8 weeks) -R7 (measured at week 0).

TABLE 8 test group Effect

As seen from the results of table 8 and fig. 5, the skin elasticity ability was improved: example 1> comparative examples 1 to 7.

The cream composition containing the liposome for improving the skin elasticity can effectively improve the skin elasticity, and has two main reasons that three functional components, namely, a fucus rudis extract, a palmitoyl tripeptide-5/dunaliella salina/sodium hyaluronate/provitamin B5 compound and bird's nest acid are compounded and selected, so that the skin cream composition can promote the synthesis of skin collagen and elastin, protect a supporting structure in a skin dermis layer, provide moisture for the skin and enable the skin to be full and elastic, wherein the fucus rudis extract can provide certain nutrition for the skin and promote the synthesis of collagen; the palmitoyl tripeptide-5/dunaliella salina/sodium hyaluronate/provitamin B5 compound can supplement skin moisture and hyaluronic acid to increase skin plumpness; the cubilose acid can effectively remove excessive free radicals in the skin so as to protect collagen and elastin in the dermis layer of the skin.

In addition, the liposome is used as a delivery carrier of the functional components, so that the stability of the functional components can be maintained, the transdermal efficiency of the functional components can be enhanced, and the capability of the liposome for improving the skin elasticity is increased.

The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, so that any simple modification, equivalent change and modification made to the above embodiment according to the technical spirit of the present invention are within the scope of the technical solution of the present invention.