阅读说明：本技术 一种环保高效的乳酸链球菌素的提取方法 (Environment-friendly and efficient extraction method of nisin ) 是由 宋志倩 王绘砖 常国栋 王婷 于 2021-03-01 设计创作，主要内容包括：本发明提供了一种环保高效的乳酸链球菌素的提取方法,包括如下步骤：A、一次陶瓷膜过滤：将乳酸链球菌素发酵液进行一次陶瓷膜过滤浓缩,得到第一浓缩液；B、二次陶瓷膜过滤：将步骤A中得到的第一浓缩液加水稀释至原发酵液体积的0.4-0.6倍后进行酸化,采用蒸汽加热后进行二次陶瓷膜过滤,得到第二浓缩液；C、陶瓷膜浓缩：将第二浓缩液采用200道尔顿的陶瓷膜浓缩,得到第三浓缩液；D、喷干：得到乳酸链球菌素成品。本发明制备的乳酸链球菌素成品含量在1000IU/mg以上,符合成品质量标准要求,收率在97％以上,高于目前其他工艺80％平均收率水平。(The invention provides an environment-friendly and efficient extraction method of nisin, which comprises the following steps: A. primary ceramic membrane filtration: performing primary ceramic membrane filtration and concentration on nisin fermentation liquor to obtain a first concentrated solution; B. secondary ceramic membrane filtration: b, adding water into the first concentrated solution obtained in the step A to dilute the first concentrated solution to 0.4-0.6 time of the volume of the original fermentation liquor, then acidifying, heating by adopting steam, and then carrying out secondary ceramic membrane filtration to obtain a second concentrated solution; C. ceramic membrane concentration: concentrating the second concentrated solution with 200 Dalton ceramic membrane to obtain third concentrated solution; D. and (3) spray drying: and obtaining the finished product of nisin. The content of the finished product of the nisin prepared by the invention is more than 1000IU/mg, the nisin meets the requirement of the quality standard of the finished product, and the nisin has the yield of more than 97 percent, which is higher than the average yield level of 80 percent of other prior processes.)

1. An environment-friendly and efficient extraction method of nisin is characterized by comprising the following steps: the method comprises the following steps:

A. primary ceramic membrane filtration: performing primary ceramic membrane filtration and concentration on nisin fermentation liquor to obtain a first concentrated solution;

B. secondary ceramic membrane filtration: b, adding water into the first concentrated solution obtained in the step A to dilute the first concentrated solution to 0.4-0.6 time of the volume of the original fermentation broth, then acidifying, heating by adopting steam, then carrying out secondary ceramic membrane filtration, wherein the adopted ceramic membrane is a 2000 Dalton membrane, and stopping concentration when the concentration rate is lower than 20-25L/h to obtain a second concentrated solution;

C. ceramic membrane concentration: concentrating the second concentrated solution obtained in the step B by adopting a ceramic membrane of 200 daltons to obtain a third concentrated solution;

D. and (3) spray drying: and D, adding edible salt according to the external titer and the dry solid content of the third concentrated solution chamber obtained in the step C, and then spraying and drying by using a spray drying tower to obtain a nisin finished product.

2. The method for extracting nisin with environmental protection and high efficiency according to claim 1, wherein the method comprises the following steps: and C, performing primary ceramic membrane filtration in the step A, wherein the adopted ceramic membrane is a 2000 Dalton membrane, and the concentration multiple is 5-8 times.

3. The method for extracting nisin with environmental protection and high efficiency according to claim 1, wherein the method comprises the following steps: the pH value of acidification in the step B is 2.0-3.5, and the steam heating temperature is 70-85 ℃.

4. The method for extracting nisin with environmental protection and high efficiency according to claim 1, wherein the method comprises the following steps: the concentration titer control range in the step C is 15-20 ten thousand IU/mL.

5. The method for extracting nisin with environmental protection and high efficiency according to claim 1, wherein the method comprises the following steps: the inlet air temperature of the spray drying tower is controlled to be 120-150 ℃, and the outlet air temperature is controlled to be 70-90 ℃.

Technical Field

The invention belongs to the field of food preservatives, and particularly relates to an environment-friendly and efficient extraction method of nisin.

Background

Nisin is a safe, nontoxic and efficient natural food preservative. Can be used for preserving meat products, dairy products, vegetable protein food, canned food, etc. Nisin solves the problem of short-time low-temperature sterilization and fundamentally improves the taste of food.

The food additive joint expert committee of the united nations food and agriculture organization and the world health organization (FAO/WHO) in 1969 confirmed that nisin could be a food preservative, and the us FDA (us food and drug administration) confirmed nisin as a GRAS (generally recognized as safe) food additive in 1988.

At present, the industrial production of nisin mainly adopts a fermentation method, and the method for extracting nisin from fermentation liquor mainly comprises the following steps: organic solvent extraction, salting out, resin separation, etc.

However, the above methods all have certain disadvantages, such as: the organic solvent extraction method needs a large amount of organic reagents, such as acetone, ethanol, methanol, propanol and the like, and the use of a large amount of solvents has certain dangerousness and great harm to the environment. The salting-out method cannot effectively remove proteins in the feed liquid, and the quality and the state of the product are affected. The resin used in the resin method is expensive, a large amount of organic solvent is used, the process is complex, and the potential safety hazard problem exists. The above processes are not optimal for industrial production.

Disclosure of Invention

In order to solve the technical problems, the invention provides a simple and practical extraction process which is characterized by simple process, strong operability, little environmental pollution, low cost, high yield and stable product quality.

The technical scheme adopted by the invention is as follows: an environment-friendly and efficient extraction method of nisin comprises the following steps:

A. primary ceramic membrane filtration: performing primary ceramic membrane filtration and concentration on nisin fermentation liquor to obtain a first concentrated solution;

B. secondary ceramic membrane filtration: b, adding water into the first concentrated solution obtained in the step A to dilute the first concentrated solution to 0.4-0.6 time of the volume of the original fermentation broth, then acidifying, heating by adopting steam, then carrying out secondary ceramic membrane filtration, wherein the adopted ceramic membrane is a 2000 Dalton membrane, and stopping concentration when the concentration rate is lower than 20-25L/h to obtain a second concentrated solution;

C. ceramic membrane concentration: concentrating the second concentrated solution obtained in the step B by adopting a ceramic membrane of 200 daltons to obtain a third concentrated solution;

D. and (3) spray drying: and D, adding edible salt according to the external titer and the dry solid content of the third concentrated solution chamber obtained in the step C, and then spraying and drying by using a spray drying tower to obtain a nisin finished product.

Further, in the step A, a ceramic membrane is used for filtering for the first time, the adopted ceramic membrane is a 2000 Dalton membrane, and the concentration multiple is 5-8 times.

Further, the pH value of acidification in the step B is 2.0-3.5, and the steam heating temperature is 70-85 ℃.

Further, the concentration titer in the step C is controlled within the range of 15-20 ten thousand IU/mL.

Further, the inlet air temperature of the spray drying tower is controlled to be 120-150 ℃, and the outlet air temperature is controlled to be 70-90 ℃.

Compared with the prior art, the invention has the advantages that: the working efficiency is improved, the process flow is simplified, the workload is reduced, the overall yield and the efficiency are improved, and the yield of the finished product prepared by the process is over 97 percent and is higher than the technical level of the prior industry; the process has no salting-out process, no high-salt sewage is generated, and the sewage treatment cost is very low; the invention has the advantages of less equipment investment, easy operation, little environmental pollution and simple operation, and is suitable for industrial production of products.

The content of the finished product of the nisin prepared by the invention is more than 1000IU/mg, the nisin meets the requirement of the quality standard of the finished product, and the nisin has the yield of more than 97 percent, which is higher than the average yield level of 80 percent of other prior processes.

Detailed Description

The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments.

An environment-friendly and efficient extraction method of nisin comprises the following steps: A. primary ceramic membrane filtration: performing primary ceramic membrane filtration and concentration on nisin fermentation liquor to obtain a first concentrated solution; B. secondary ceramic membrane filtration: b, adding water into the first concentrated solution obtained in the step A to dilute the first concentrated solution to 0.4-0.6 time of the volume of the original fermentation broth, then acidifying, heating by adopting steam, then carrying out secondary ceramic membrane filtration, wherein the adopted ceramic membrane is a 2000 Dalton membrane, and stopping concentration when the concentration rate is lower than 20-25L/h to obtain a second concentrated solution; C. ceramic membrane concentration: concentrating the second concentrated solution obtained in the step B by adopting a ceramic membrane of 200 daltons to obtain a third concentrated solution; D. and (3) spray drying: and D, adding edible salt according to the external titer and the dry solid content of the third concentrated solution chamber obtained in the step C, and then spraying and drying by using a spray drying tower to obtain a nisin finished product.

And C, performing primary ceramic membrane filtration in the step A, wherein the adopted ceramic membrane is a 2000 Dalton membrane, and the concentration multiple is 5-8 times. The pH value of acidification in the step B is 2.0-3.5, and the steam heating temperature is 70-85 ℃. The concentration titer control range in the step C is 15-20 ten thousand IU/mL. The inlet air temperature of the spray drying tower is controlled to be 120-150 ℃, and the outlet air temperature is controlled to be 70-90 ℃.

Example 1

100L of nisin fermentation liquor (fermentation titer 9000IU/mL) is taken, a 2000 Dalton membrane is adopted for primary ceramic membrane filtration, and the yield is about 99.2%. Diluting the concentrated solution to 18010IU/mL, acidifying with hydrochloric acid to pH 2.83, heating to 78 deg.C, filtering with 2000 Dalton membrane to obtain a secondary ceramic membrane filtrate with yield of 98.9%, concentrating the secondary ceramic membrane filtrate with 200 Dalton membrane to volume of 5.8L, concentrating the concentrated solution to obtain concentrated solution with titer of 15.12 ten thousand IU/mL, dry solid content of 31mg/mL and concentration yield of 99.3%, calculating salt adding amount according to the concentrated solution titer and dry solid content, adding 697.16g of edible salt into the concentrated solution, and spray drying.

The content of the finished product prepared by the batch is 1031IU/mg, and the yield is 97.42%.

Example 2

100L of nisin fermentation liquor (fermentation titer 9000IU/mL) is taken, a 2000 Dalton membrane is adopted for primary ceramic membrane filtration, and the yield is 99.3%. After the concentrated solution is diluted, the titer is 19032 Iu/mL, the pH value is acidified to 2.71 by hydrochloric acid, the heating temperature is 78 ℃, secondary ceramic membrane filtration is carried out by adopting a 2000 Dalton membrane, the yield is 98.8%, secondary ceramic membrane filtrate is concentrated to the volume of 5.4L by adopting a 200 Dalton ceramic membrane, the titer of the concentrated solution is 16.27IU/mL, the dry solid content is 38.42mg/mL, the concentration yield is 99.5%, the salt adding amount is calculated according to the titer and the dry solid content of the concentrated solution, 671.11g of edible salt is added into the concentrated solution, and the concentrated solution is sprayed and dried.

The content of finished products prepared by the batch is 1021IU/mg, and the yield is 97.62%.

Example 3

100L of nisin fermentation liquor (fermentation titer 9000IU/mL) is taken, a 2000 Dalton membrane is adopted for primary ceramic membrane filtration, and the yield is 98.7%. Diluting the concentrated solution to obtain a titer of 16830Iu/mL, acidifying with hydrochloric acid to obtain a pH value of 2.96, heating to 82 ℃, performing secondary ceramic membrane filtration by using a 2000 Dalton membrane to obtain a yield of 98.9%, concentrating the secondary ceramic membrane filtrate to a volume of 4.97L by using a 200 Dalton ceramic membrane to obtain a concentrated solution titer of 17.55IU/mL, a dry solid content of 40.16mg/mL and a concentration yield of 99.3%, calculating a salt adding amount according to the concentrated solution titer and the dry solid content, adding 672.64g of edible salt into the concentrated solution, and performing spray drying.

The finished product content of this batch was 1036IU/mg, with a yield of 96.93%.

Example 4

100L of nisin fermentation liquor (fermentation titer 9000IU/mL) is taken, a 2000 Dalton membrane is adopted for primary ceramic membrane filtration, and the yield is 98.93%. After the concentrated solution is diluted, the titer is 16391IU/mL, the pH value is acidified to 3.24 by hydrochloric acid, the heating temperature is 81 ℃, secondary ceramic membrane filtration is carried out by adopting a 2000 Dalton membrane, the yield is 99.4%, secondary ceramic membrane filtrate is concentrated to 4.68L in volume by adopting a 200 Dalton ceramic membrane, the titer of the concentrated solution is 18.68 ten thousand IU/mL, the dry solid content is 41.64mg/mL, the concentration yield is 98.8%, the salt adding amount is calculated according to the titer and the dry solid content of the concentrated solution, 679.35g of edible salt is added into the concentrated solution, and spray drying is carried out.

The content of the finished product prepared by the batch is 1024IU/mg, and the yield is 97.15%.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.