Alkaline electrolyzed water synergistic liquid and preparation method and application thereof

文档序号：539618 发布日期：2021-06-04 浏览：6次中文

阅读说明：本技术 一种碱性电解水增效液及其制备方法和应用 (Alkaline electrolyzed water synergistic liquid and preparation method and application thereof ) 是由闫学海沈桂芝刘彦辰邢蕊蕊袁成前于 2021-02-07 设计创作，主要内容包括：本发明涉及一种碱性电解水增效液及其制备方法和应用。所述碱性电解水增效液包括碱性电解水和增效物,所述增效物包括壳聚糖和/或壳聚糖衍生物。所述碱性电解水增效液的制备方法包括：按比例将碱性电解水与增效物混合,并进行降温处理,得到所述碱性电解水增效液。所述碱性电解水增效液中,碱性电解水与增效物相互配合,使得所述碱性电解水增效液具备高效、长效的抑菌效果。(The invention relates to an alkaline electrolyzed water synergistic solution and a preparation method and application thereof. The alkaline electrolyzed water synergistic fluid comprises alkaline electrolyzed water and a synergistic product, wherein the synergistic product comprises chitosan and/or a chitosan derivative. The preparation method of the alkaline electrolyzed water synergistic solution comprises the following steps: and mixing the alkaline electrolyzed water and the synergist in proportion, and cooling to obtain the alkaline electrolyzed water synergistic solution. In the alkaline electrolyzed water synergistic solution, alkaline electrolyzed water and a synergist are matched with each other, so that the alkaline electrolyzed water synergistic solution has a high-efficiency and long-acting bacteriostatic effect.)

1. An alkaline electrolyzed water synergistic fluid is characterized by comprising alkaline electrolyzed water and a synergist;

the potentiator comprises chitosan and/or a chitosan derivative.

2. The alkaline electrolyzed water synergistic solution according to claim 1, characterized in that the alkaline electrolyzed water comprises alkaline electrolyzed water obtained by electrolysis from any electrolyte;

preferably, the pH value of the alkaline electrolyzed water is 10.2-12.8, and preferably 11.0-12.8.

3. The alkaline electrolyzed water enhancing solution according to claim 1 or 2, wherein the chitosan derivative is a water-soluble chitosan derivative;

preferably, the chitosan derivative comprises any one of chitosan quaternary ammonium salt, 2-hydroxypropyl trimethyl ammonium chloride chitosan or carboxymethyl chitosan or the combination of at least two of the chitosan quaternary ammonium salt, the 2-hydroxypropyl trimethyl ammonium chloride chitosan and the carboxymethyl chitosan.

4. The alkaline electrolyzed water enhancing solution according to any one of claims 1 to 3, wherein the molecular weights of the chitosan and the chitosan derivative are each independently 3 to 100 kDa.

5. The alkaline electrolyzed water synergistic solution according to any one of claims 1 to 4, wherein the concentration of the synergistic substance in the alkaline electrolyzed water synergistic solution is 0.1 to 100mg/mL, preferably 1 to 50mg/mL, and more preferably 1 to 20 mg/mL.

6. A method for preparing an alkaline electrolyzed water enhancing solution as defined in any one of claims 1 to 5, comprising:

and mixing the alkaline electrolyzed water and the synergist in proportion, and cooling to obtain the alkaline electrolyzed water synergistic solution.

7. The method of claim 6, wherein the mixing temperature is 20-60 ℃, preferably 20-40 ℃;

preferably, the mixing time is 0.5-12 h, preferably 1-6 h, and more preferably 2-4 h.

8. The preparation method according to claim 6 or 7, wherein the temperature reduction treatment is to reduce the temperature to 0-10 ℃, preferably to 3-6 ℃;

preferably, the time of the temperature reduction treatment is 1-72 hours, preferably 6-48 hours, and further preferably 12-48 hours.

9. The production method according to any one of claims 6 to 8, characterized by comprising:

mixing alkaline electrolyzed water and a synergist in proportion at the temperature of 20-60 ℃, and treating for 1-72 hours at the temperature of 0-10 ℃ to obtain the alkaline electrolyzed water synergistic solution.

10. Use of an alkaline electrolyzed water enhancing solution according to any one of claims 1-5 in the preparation of a disinfectant.

Technical Field

The invention belongs to the technical field of daily chemical industry, and relates to an alkaline electrolyzed water synergistic solution and a preparation method and application thereof.

Background

The alkaline electrolyzed water is novel functional water, has the characteristics of good bactericidal performance, strong permeability, no residue, no stimulation, no cleaning, safety and no toxicity, is widely applied to the aspects of medicine, agriculture, daily chemicals, food, families and the like, and is particularly suitable for application scenes of wiping and washing.

The sterilization performance of the alkaline electrolyzed water completely depends on the pH value, and the optimal sterilization effect can be achieved by generally maintaining the pH value to be more than 12. However, in practice, alkaline electrolyzed water is susceptible to open conditions (e.g., carbon dioxide in air) and quickly becomes completely ineffective. Research on Wangxiping and the like shows that the pH value of alkaline electrolyzed water changes rapidly due to the surrounding environment and the nature of the alkaline electrolyzed water, and the alkaline electrolyzed water basically fails after being placed for 3 days (Wangxiping, Zhanying, Populan, and the like. preparation research on alkaline electrolyzed water [ J ]. Shandong chemical industry, 2015, 44 (1): 38-39.). Although the alkaline electrolyzed water stored under the sealed condition can be stored for more than one month, the sealed storage condition is difficult to ensure after people start to use in daily household scenes. In other cases with acidic buffers, alkaline electrolyzed water is also almost completely ineffective due to its poor buffering capacity.

In summary, how to provide alkaline electrolyzed water capable of effectively enduring environmental influences and maintain long-acting antibacterial effect has become one of the problems to be solved in the field of alkaline electrolyzed water.

Disclosure of Invention

Aiming at the defects and actual requirements of the prior art, the invention provides the alkaline electrolyzed water synergistic liquid and the preparation method and the application thereof.

In order to achieve the purpose, the invention adopts the following technical scheme:

in a first aspect, the present invention provides an alkaline electrolyzed water synergistic solution comprising alkaline electrolyzed water and a synergist, wherein the synergist comprises chitosan and/or a chitosan derivative.

In the alkaline electrolyzed water synergistic solution, alkaline electrolyzed water can effectively inhibit bacteria; the amino group in the chitosan carries positive charges and is easy to combine with proteins with negative charges on the surface of a bacterial cell membrane, so that the normal physiological functions of bacteria are damaged, and the bacteria die; the chitosan derivative has good water solubility, biocompatibility, degradability and broad-spectrum antibacterial activity, can form a water locking network in aqueous solution, and has moisture retention, film forming property and biological buffering capacity; the alkaline electrolyzed water and the synergist (chitosan and/or chitosan derivative) are matched with each other, so that the buffering capacity of the alkaline electrolyzed water synergist can be improved, and the alkaline electrolyzed water synergist has a high-efficiency and long-acting antibacterial effect.

Preferably, the alkaline electrolyzed water includes alkaline electrolyzed water obtained by electrolysis from any electrolytic solution.

Preferably, the pH of the alkaline electrolyzed water is 10.2 to 12.8, and may be, for example, 10.2, 10.3, 10.4, 10.5, 10.6, 10.7, 10.8, 10.9, 11, 11.1, 11.2, 11.3, 11.4, 11.5, 11.6, 11.7, 11.8, 11.9, 12, 12.1, 12.2, 12.3, 12.4, 12.5, 12.6, 12.7, or 12.8, and preferably 11.0 to 12.8.

Preferably, the chitosan derivative is a water-soluble chitosan derivative.

Preferably, the chitosan derivative comprises any one of chitosan quaternary ammonium salt, 2-hydroxypropyl trimethyl ammonium chloride chitosan or carboxymethyl chitosan or a combination of at least two of them, wherein typical but non-limiting combinations include: the chitosan quaternary ammonium salt and 2-hydroxypropyl trimethyl ammonium chloride chitosan, the chitosan quaternary ammonium salt and carboxymethyl chitosan or the 2-hydroxypropyl trimethyl ammonium chloride chitosan and carboxymethyl chitosan are preferably chitosan quaternary ammonium salt.

Preferably, the molecular weight of the chitosan and chitosan derivative is each independently 3-100 kDa, for example 3kDa, 5kDa, 7kDa, 10kDa, 15kDa, 20kDa, 25kDa, 30kDa, 35kDa, 40kDa, 45kDa, 50kDa, 55kDa, 60kDa, 65kDa, 70kDa, 75kDa, 80kDa, 85kDa, 90kDa, 95kDa or 100 kDa.

Preferably, the concentration of the potentiator in the alkaline electrolyzed water potentiator solution is 0.1 to 100mg/mL, and may be, for example, 0.1mg/mL, 0.2mg/mL, 0.3mg/mL, 0.4mg/mL, 0.5mg/mL, 0.6mg/mL, 0.8mg/mL, 1mg/mL, 2mg/mL, 3mg/mL, 4mg/mL, 5mg/mL, 6mg/mL, 8mg/mL, 10mg/mL, 15mg/mL, 20mg/mL, 25mg/mL, 30mg/mL, 35mg/mL, 40mg/mL, 50mg/mL, 60mg/mL, 70mg/mL, 80mg/mL, 90mg/mL, or 100mg/mL, preferably 1 to 50mg/mL, and more preferably 1 to 20 mg/mL.

In a second aspect, the present invention provides a method for preparing the alkaline electrolyzed water synergistic fluid as described in the first aspect, the method comprising:

and mixing the alkaline electrolyzed water and the synergist in proportion, and cooling to obtain the alkaline electrolyzed water synergistic solution.

Preferably, the mixing temperature is 20 ~ 60 ℃, for example can be 20 ℃, 22 ℃, 23 ℃, 25 ℃, 30 ℃, 35 ℃, 40 ℃, 45 ℃, 50 ℃, 55 ℃ or 60 ℃, preferably 20 ~ 40 ℃.

Preferably, the mixing time is 0.5 to 12 hours, for example, 0.5 hour, 1 hour, 2 hours, 3 hours, 4 hours, 5 hours, 6 hours, 7 hours, 8 hours, 9 hours, 10 hours and 12 hours, preferably 1 to 6 hours, and more preferably 2 to 4 hours;

preferably, the temperature reduction treatment is carried out to reduce the temperature to 0 to 10 ℃, for example, 0 ℃, 1 ℃, 2 ℃, 3 ℃, 4 ℃, 5 ℃, 6 ℃, 7 ℃, 8 ℃, 9 ℃ or 10 ℃, preferably 3 to 6 ℃.

Preferably, the time of the temperature reduction treatment is 1 to 72 hours, for example, 1 hour, 2 hours, 3 hours, 4 hours, 5 hours, 6 hours, 7 hours, 8 hours, 9 hours, 10 hours, 12 hours, 18 hours, 20 hours, 24 hours, 48 hours and 72 hours, preferably 6 to 48 hours, and more preferably 12 to 48 hours.

As a preferable technical scheme, the preparation method of the alkaline electrolyzed water synergistic solution comprises the following steps:

In a third aspect, the invention provides the use of an alkaline electrolyzed water enhancing solution as defined in the first aspect in the preparation of a disinfectant.

Compared with the prior art, the invention has the following beneficial effects:

(1) in the alkaline electrolyzed water synergistic solution, alkaline electrolyzed water and a synergist (chitosan and/or a chitosan derivative) are matched with each other, so that the alkaline electrolyzed water synergistic solution has a high-efficiency and long-acting bacteriostatic effect;

(2) the alkaline electrolyzed water synergistic solution has the advantages of simple production process, environmental protection, rich raw material source, low cost and suitability for wide popularization;

(3) the alkaline electrolyzed water synergistic solution has long-acting antibacterial effect, and the antibacterial efficiency reduction rate is lower than 8.4 percent after the alkaline electrolyzed water synergistic solution is placed for 3 months.

Drawings

FIG. 1 shows the inhibition zones of the alkaline electrolyzed water synergistic solution prepared in example 7 and the alkaline electrolyzed water prepared in comparative example 3 against Escherichia coli and Staphylococcus aureus;

FIG. 2 is a graph showing the bacterial killing rate of the alkaline electrolyzed water enhancing solution prepared in example 4 and the alkaline electrolyzed water of comparative example 4.

Detailed Description

To further illustrate the technical means adopted by the present invention and the effects thereof, the present invention is further described below with reference to the embodiments and the accompanying drawings. It is to be understood that the specific embodiments described herein are merely illustrative of the invention and are not limiting of the invention.

The examples do not show the specific techniques or conditions, according to the technical or conditions described in the literature in the field, or according to the product specifications. The reagents or apparatus used are conventional products commercially available from normal sources, not indicated by the manufacturer.

In the examples of the present invention, alkaline electrolyzed water was prepared according to the literature [ Wangxueping, et al. Research on preparation of strong alkaline electrolyzed water, Shandong chemical engineering, volume 44 of 2015, 38-41] and chitosan, chitosan quaternary ammonium salt, 2-hydroxypropyl trimethyl ammonium chloride chitosan and carboxymethyl chitosan are all purchased from Bailingwei science and technology Limited.

Example 1